BO87DRAFT_380030 cDNA ORF clone,

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gRNAs

The following BO87DRAFT_380030 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BO87DRAFT_380030 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAs248139	XM_025624203.1 Latest version!	Aspergillus neoniger CBS 115656 histidine-tagged glucoamylase (BO87DRAFT_380030), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BO87DRAFT_380030 ( XM_025624203.1 ) cDNA ORF clone, -> XP_025475673.1 Aspergillus neoniger CBS 115656 histidine-tagged glucoamylase (BO87DRAFT_380030), mRNA.

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CloneID	OAs248139
Clone ID Related Accession (Same CDS sequence)	XM_025624203.1
Accession Version	XM_025624203.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-05
Organism	Aspergillus neoniger CBS 115656
Product	histidine-tagged glucoamylase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_020291167). ##Metadata-START## Organism Display Name :: Aspergillus neoniger CBS 115656 v1.0 GOLD Stamp ID :: Gp0047284 ##Metadata-END##

ATGTCGTTCC GATCTCTTCT CGCCCTGAGT GGCCTTGTCT GCTCGGGGTT GGCAAGTGTT 
ATTTCCAAGC GCGCGACCTT GGATTCGTGG TTGAGCAACG AAGCGACCGT GGCTCGTACT 
GCGATCCTGA ATAACATCGG GGCGGACGGT GCTTGGGTGT CGGGCGCGGA CTCGGGCATT 
GTCGTTGCTA GTCCCAGCAC CGATAACCCG GACTACTTCT ACACCTGGAC TCGCGACTCT 
GGTCTCGTCA TCAAGACCCT CGTCGATCTC TTCCGCAATG GAGATACTGA TCTCCTTTCC 
ACCATTGAGA ACTACATCTC CTCTCAGGCA ATTGTTCAGG GTATCAGTAA CCCCTCTGGT 
GATCTGTCCA GCGGTGGTCT TGGTGAGCCC AAGTTCAATG TCGATGAGAC TGCCTACACC 
GGGTCTTGGG GACGGCCGCA GCGTGATGGT CCTGCCCTGA GAGCAACTGC TATGATCGGC 
TTCGGGCAGT GGCTGCTTGA CAATGGCTAC ACCAGCGCTG CAACGGAGAT TGTTTGGCCC 
CTCGTTAGGA ATGACCTGTC GTATGTGGCT CAGTACTGGA ACCAGACGGG ATATGATCTC 
TGGGAAGAAG TTAATGGCTC GTCCTTCTTC ACTATTGCTG TGCAGCACCG CGCCCTCGTC 
GAAGGTAGTG CCTTCGCGAC GGCCGTCGGC TCGTCCTGCT CCTGGTGTGA TTCGCAGGCA 
CCTCAGATTC TCTGCTACCT GCAGTCCTTC TGGACCGGCA GCTACATCCT GGCCAACTTT 
GATAGCAGCC GTTCCGGCAA GGACACCAAC ACCCTCCTGG GAAGCATCCA CACCTTTGAT 
CCTGAGGCTG GGTGCGACGA CTCCACCTTC CAGCCCTGCT CCCCGCGTGC GCTCGCCAAC 
CATAAGGAGG TTGTAGACTC TTTCCGCTCG ATCTATACTC TCAACGATGG TCTCAGTGAC 
AGTGAGGCGG TTGCCGTCGG TCGGTACCCC GAGGACAGCT ACTACAACGG CAACCCGTGG 
TTCCTGTGCA CCTTGGCTGC CGCGGAGCAG CTGTACGATG CTCTGTACCA GTGGGACAAG 
CAGGGGTCGT TGGAGATCAC AGACGTGTCG CTTGACTTCT TCCAGGCTCT GTACAGTGAT 
GCTACCACTG GCACGTACTC TTCGTCCAGC TCAACCTATA GCAGCATTGT GGATGCCGTA 
AAGACTTTCG CTGATGGTTT CGTTTCTATT GTGGAAACTC ACGCCGCAAG CAACGGCTCT 
CTGTCCGAGC AATACGACAA GTCTGATGGC GACGAGCTTT CTGCTCGCGA CCTGACCTGG 
TCTTACGCTG CTCTGCTGAC CGCCAACAAC CGTCGTAACT CTGTCATGCC TCCTTCTTGG 
GGCGAGACCT CTGCCAGCAG CGTGCCCGGC ACCTGTGCGG CTACCTCTGC CTCTGGTACC 
TACAGTAGTG TGACTGTCAC CTCGTGGCCG AGCATCGTGG CTACTGGTGG CACCACTACG 
ACGGCTACCA CCACCGGATC GGGCGGCGTG ACCTCGACCA GTAAGACCAC CACAACTGCT 
AGTAAGACCA GCACCACTAC GTCCTCGACC TCCTGCACGA CTCCCACCGC CGTAGCTGTG 
ACATTTGATC TGACGGCGAC CACAACCTAC GGCGAGAACA TCTACCTGGT CGGGTCGATC 
TCTCAGCTCG GTGACTGGGA CACTAGCGAC GGCATAGCTC TGAGCGCTGA CAAGTACACT 
TCCAGCAACC CGCTCTGGTA TGTAACCGTG ACTCTGCCGG CTGGTGAGTC GTTTGAGTAC 
AAGTTCATCC GCATTGAGAG CGATGACTCC GTGGAGTGGG AGAGCGACCC GAACCGGGAA 
TACACGGTTC CTCAGGCGTG CGGAGAGTCG ACCGCGACGG TGACCGACAC CTGGCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025475673.1
CDS	239..2158
Translation	MSFRSLLALSGLVCSGLASVISKRATLDSWLSNEATVARTAILNNIGADGAWVSGADSGIVVASPSTDNPDYFYTWTRDSGLVIKTLVDLFRNGDTDLLSTIENYISSQAIVQGISNPSGDLSSGGLGEPKFNVDETAYTGSWGRPQRDGPALRATAMIGFGQWLLDNGYTSAATEIVWPLVRNDLSYVAQYWNQTGYDLWEEVNGSSFFTIAVQHRALVEGSAFATAVGSSCSWCDSQAPQILCYLQSFWTGSYILANFDSSRSGKDTNTLLGSIHTFDPEAGCDDSTFQPCSPRALANHKEVVDSFRSIYTLNDGLSDSEAVAVGRYPEDSYYNGNPWFLCTLAAAEQLYDALYQWDKQGSLEITDVSLDFFQALYSDATTGTYSSSSSTYSSIVDAVKTFADGFVSIVETHAASNGSLSEQYDKSDGDELSARDLTWSYAALLTANNRRNSVMPPSWGETSASSVPGTCAATSASGTYSSVTVTSWPSIVATGGTTTTATTTGSGGVTSTSKTTTTASKTSTTTSSTSCTTPTAVAVTFDLTATTTYGENIYLVGSISQLGDWDTSDGIALSADKYTSSNPLWYVTVTLPAGESFEYKFIRIESDDSVEWESDPNREYTVPQACGESTATVTDTWR

Target ORF information:

RefSeq Version	XM_025624203.1
Organism	Aspergillus neoniger CBS 115656
Definition	Aspergillus neoniger CBS 115656 histidine-tagged glucoamylase (BO87DRAFT_380030), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025624203.1

ORF Insert Sequence:

ATGTCGTTCC GATCTCTTCT CGCCCTGAGT GGCCTTGTCT GCTCGGGGTT GGCAAGTGTT 
ATTTCCAAGC GCGCGACCTT GGATTCGTGG TTGAGCAACG AAGCGACCGT GGCTCGTACT 
GCGATCCTGA ATAACATCGG GGCGGACGGT GCTTGGGTGT CGGGCGCGGA CTCGGGCATT 
GTCGTTGCTA GTCCCAGCAC CGATAACCCG GACTACTTCT ACACCTGGAC TCGCGACTCT 
GGTCTCGTCA TCAAGACCCT CGTCGATCTC TTCCGCAATG GAGATACTGA TCTCCTTTCC 
ACCATTGAGA ACTACATCTC CTCTCAGGCA ATTGTTCAGG GTATCAGTAA CCCCTCTGGT 
GATCTGTCCA GCGGTGGTCT TGGTGAGCCC AAGTTCAATG TCGATGAGAC TGCCTACACC 
GGGTCTTGGG GACGGCCGCA GCGTGATGGT CCTGCCCTGA GAGCAACTGC TATGATCGGC 
TTCGGGCAGT GGCTGCTTGA CAATGGCTAC ACCAGCGCTG CAACGGAGAT TGTTTGGCCC 
CTCGTTAGGA ATGACCTGTC GTATGTGGCT CAGTACTGGA ACCAGACGGG ATATGATCTC 
TGGGAAGAAG TTAATGGCTC GTCCTTCTTC ACTATTGCTG TGCAGCACCG CGCCCTCGTC 
GAAGGTAGTG CCTTCGCGAC GGCCGTCGGC TCGTCCTGCT CCTGGTGTGA TTCGCAGGCA 
CCTCAGATTC TCTGCTACCT GCAGTCCTTC TGGACCGGCA GCTACATCCT GGCCAACTTT 
GATAGCAGCC GTTCCGGCAA GGACACCAAC ACCCTCCTGG GAAGCATCCA CACCTTTGAT 
CCTGAGGCTG GGTGCGACGA CTCCACCTTC CAGCCCTGCT CCCCGCGTGC GCTCGCCAAC 
CATAAGGAGG TTGTAGACTC TTTCCGCTCG ATCTATACTC TCAACGATGG TCTCAGTGAC 
AGTGAGGCGG TTGCCGTCGG TCGGTACCCC GAGGACAGCT ACTACAACGG CAACCCGTGG 
TTCCTGTGCA CCTTGGCTGC CGCGGAGCAG CTGTACGATG CTCTGTACCA GTGGGACAAG 
CAGGGGTCGT TGGAGATCAC AGACGTGTCG CTTGACTTCT TCCAGGCTCT GTACAGTGAT 
GCTACCACTG GCACGTACTC TTCGTCCAGC TCAACCTATA GCAGCATTGT GGATGCCGTA 
AAGACTTTCG CTGATGGTTT CGTTTCTATT GTGGAAACTC ACGCCGCAAG CAACGGCTCT 
CTGTCCGAGC AATACGACAA GTCTGATGGC GACGAGCTTT CTGCTCGCGA CCTGACCTGG 
TCTTACGCTG CTCTGCTGAC CGCCAACAAC CGTCGTAACT CTGTCATGCC TCCTTCTTGG 
GGCGAGACCT CTGCCAGCAG CGTGCCCGGC ACCTGTGCGG CTACCTCTGC CTCTGGTACC 
TACAGTAGTG TGACTGTCAC CTCGTGGCCG AGCATCGTGG CTACTGGTGG CACCACTACG 
ACGGCTACCA CCACCGGATC GGGCGGCGTG ACCTCGACCA GTAAGACCAC CACAACTGCT 
AGTAAGACCA GCACCACTAC GTCCTCGACC TCCTGCACGA CTCCCACCGC CGTAGCTGTG 
ACATTTGATC TGACGGCGAC CACAACCTAC GGCGAGAACA TCTACCTGGT CGGGTCGATC 
TCTCAGCTCG GTGACTGGGA CACTAGCGAC GGCATAGCTC TGAGCGCTGA CAAGTACACT 
TCCAGCAACC CGCTCTGGTA TGTAACCGTG ACTCTGCCGG CTGGTGAGTC GTTTGAGTAC 
AAGTTCATCC GCATTGAGAG CGATGACTCC GTGGAGTGGG AGAGCGACCC GAACCGGGAA 
TACACGGTTC CTCAGGCGTG CGGAGAGTCG ACCGCGACGG TGACCGACAC CTGGCGGTAG