DBE1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	DBE1
Entrez Gene ID	839531
Full Name	debranching enzyme 1
Synonyms	ARABIDOPSIS THALIANA ISOAMYLASE 2,ATISA2,AtBE2,BE2,BRANCHING ENZYME 2,F15K9.9,F15K9_9,ISA2,debranching enzyme 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_202022.3	NP_973751.1	debranching enzyme 1
NM_100213.4	NP_171830.1	debranching enzyme 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Endogenous starch debranching enzymes ISA1 and ISA2 of Arabidopsis are conserved among diverse groups of plants.
Title: Replacement of the endogenous starch debranching enzymes ISA1 and ISA2 of Arabidopsis with the rice orthologs reveals a degree of functional conservation during starch synthesis.

Data indicate that analysis of recombinant enzymes showed that Data iindicate that Arabidopsis ISA1 requires ISA2 as a partner for enzymatic function, whereas maize ISA1 was active by itself.
Title: Distinct functional properties of isoamylase-type starch debranching enzymes in monocot and dicot leaves.

The heteromultimeric debranching enzyme involved in starch synthesis in Arabidopsis requires both isoamylase1 and isoamylase2 subunits for complex stability and activity.
Title: The heteromultimeric debranching enzyme involved in starch synthesis in Arabidopsis requires both isoamylase1 and isoamylase2 subunits for complex stability and activity.

Data propose that ISA2 function in normal amylopectin synthesis by promoting amylopectin crystallization but conclude that they are not mandatory for starch granule synthesis.
Title: Starch granule biosynthesis in Arabidopsis is abolished by removal of all debranching enzymes but restored by the subsequent removal of an endoamylase.

The following DBE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DBE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb24853	NM_100213.4 Latest version!	Arabidopsis thaliana debranching enzyme 1 (DBE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb24853	NM_202022.3 Latest version!	Arabidopsis thaliana debranching enzyme 1 (DBE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DBE1 ( NM_202022.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_973751.1 Arabidopsis thaliana debranching enzyme 1 (DBE1), mRNA.

Price & Availability↑

CloneID	OAb24853
Clone ID Related Accession (Same CDS sequence)	NM_202022.3 , NM_100213.4
Accession Version	NM_202022.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2649bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	debranching enzyme 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_202022.2.

ATGGCTGCGT GGTCACCATC AGTTGGGATA GGATCTTGTT GTCTCAATAA TGGCATCACT 
AGAACATGGA AGTTTCCTTC TGCAAGGCTT TTTACTGGTA GGAAGAACAA GATTAAACTT 
GGATCCGAGA CACTAATGTT TACACGAAAA CGTTTCATGG GTGATTTGGT TACAAGCGCG 
TTGCAAAGTT ATCAGTTTAG TAAGATATGT GCTTCAAAGA CTTCCATCGA GCTGAGAGAG 
GCATTGTCTA GTAGAAGAGC TGAGGCTGAT GACCTGAAGA AGGTAACATC ATATTCATTC 
AGGACAAAAG CAGGTGCTCT GGTTAAAGTC AAAGTTGAAA AGAAGAGAGA AAAGTACAGC 
ATTTTGGTTT ATGTCTCATC TTTGGAACTT AGTGGTGATG ACAAGAGTAG GCTGGTGATG 
GTTTGGGGTG TCTACAGGTC TGATTCTTCG TGTTTTTTGC CTCTGGATTT CGAAAACTCT 
TCCCAAGACT CACAAACCCA CACAACAGAA ACTACATTTG TGAAAAGCTC CTTGTCTGAG 
TTAATGCTAG GGCTAGAATT TGATGGGAAA GAATCTCCTT TCTACTTATC ATTTCACCTG 
AAGTTAGTGT CAGGTAGAGA TCCGGACGGT CAAGAAATGC TGACTCACAG GGACACTGAC 
TTCTGTATCC CGGTTGGTTT TACTGCTGGT CATCCATTGC CGCTAGGCCT TTCTTCCGGG 
CCAGATGATG ATTCTTGGAA TTTTTCGTTC TTTTCAAGAA GCTCAACGAA CGTGGTTTTA 
TGCTTGTATG ATGATAGCAC AACAGATAAA CCTGCTTTAG AACTTGATCT TGATCCTTAT 
GTCAACCGAA CAGGTGATGT TTGGCATGCT TCAGTTGACA ATACATGGGA TTTTGTGAGA 
TATGGCTACC GTTGCAAGGA GACAGCTCAC TCTAAAGAAG ATGTCGATGT TGAAGGTGAG 
CCAATTGTCT TGGATCCATA TGCCACAGTC GTTGGTAAAT CTGTCTCTCA GAAATATCTT 
GGAAGTTTAT CTAAGAGTCC TTCTTTTGAT TGGGGAGAAG ATGTGTCTCC GAACATACCT 
CTGGAGAAAC TTCTTGTTTA CCGGTTGAAT GTGAAGGGTT TTACACAACA CAGATCCAGC 
AAGTTGCCTA GTAACGTAGC AGGGACATTT TCTGGTGTAG CTGAGAAAGT TAGCCATTTG 
AAAACCCTTG GAACCAACGC TGTTCTTTTG GAGCCAATAT TCTCATTCTC CGAGCAAAAA 
GGTCCTTATT TTCCATTTCA TTTCTTTTCA CCAATGGACA TATATGGACC TTCCAATAGC 
CTTGAATCCG CAGTTAACTC AATGAAAGTG ATGGTGAAGA AATTGCACAG TGAGGGAATA 
GAGGTACTTT TGGAAGTAGT TTTTACACAT ACTGCTGATT CTGGAGCTCT TCGTGGAATT 
GATGACAGTT CCTATTACTA CAAGGGAAGA GCCAATGATC TAGATTCTAA AAGTTACTTG 
AACTGTAACT ATCCTGTTGT TCAGCAGTTG GTATTGGAGA GCTTGCGTTA TTGGGTAACC 
GAGTTTCATG TAGATGGATT TTGTTTTATA AATGCTTCAT CTCTCTTGAG AGGCGTTCAC 
GGTGAACAGC TCTCTCGTCC TCCTTTGGTT GAAGCAATAG CTTTTGATCC ACTTCTTGCG 
GAGACCAAAC TAATAGCTGA TTGCTGGGAT CCACTTGAAA TGATGCCAAA AGAAGTACGG 
TTCCCACATT GGAAGCGATG GGCAGAACTC AACACAAGAT ATTGTCGAAA TGTAAGAAAT 
TTTTTAAGGG GAAGAGGCGT TCTTAGTGAT CTGGCTACAA GAATTTGTGG AAGTGGTGAC 
GTATTTACCG ATGGAAGAGG TCCGGCCTTC TCCTTCAACT ACATTTCAAG AAACTCAGGA 
CTCTCGCTTG TTGACATAGT CAGTTTCAGT GGCCCTGAGC TAGCTTCAGA GCTAAGTTGG 
AATTGTGGGG AAGAAGGAGC AACGAATAAA TCAGCTGTTC TTCAAAGAAG GCTAAAACAA 
ATCCGCAATT TCTTGTTTAT ACAGTACATA TCACTTGGAG TTCCAGTGCT TAATATGGGA 
GATGAATGTG GAATCTCGAC AAGGGGCTCG CCTTTGTTGG AATCACGGAA ACCTTTTGAT 
TGGAACTTGC TAGCTTCGGC TTTCGGTACA CAGATCACGC AATTTATCTC CTTCATGACT 
TCAGTAAGAG CAAGAAGAAG TGATGTGTTT CAAAGGAGAG ACTTTTTGAA ACCTGAAAAT 
ATTGTTTGGT ATGCAAATGA CCAAACAACT CCAAAGTGGG AAGATCCTGC TTCTAAGTTT 
TTGGCATTGG AGATCAAATC AGAGTCAGAG GAAGAGGAAA CTGCGTCATT GGCTGAGCCA 
AATGAGCCAA AGAGCAATGA TCTGTTCATC GGATTCAATG CGAGTGATCA TCCTGAGAGT 
GTAGTCTTAC CTTCACTTCC CGATGGAAGC AAATGGAGAA GATTGGTCGA CACAGCTCTT 
CCTTTCCCGG GGTTTTTCTC TGTCGAAGGA GAAACCGTTG TAGCAGAAGA ACCGTTGCAG 
CAACTGGTTG TGTATGAGAT GAAGCCGTAC AGTTGTACCC TTTTTGAAAC CATCAATACT 
ACCGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_973751.1
CDS	239..2887
Translation	MAAWSPSVGIGSCCLNNGITRTWKFPSARLFTGRKNKIKLGSETLMFTRKRFMGDLVTSALQSYQFSKICASKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSILVYVSSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELMLGLEFDGKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVVGKSVSQKYLGSLSKSPSFDWGEDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFSFSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSGALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYCRNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGPELASELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLESRKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWEDPASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRLVDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFETINTTA

Target ORF information:

RefSeq Version	NM_202022.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana debranching enzyme 1 (DBE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_202022.3

ORF Insert Sequence:

ATGGCTGCGT GGTCACCATC AGTTGGGATA GGATCTTGTT GTCTCAATAA TGGCATCACT 
AGAACATGGA AGTTTCCTTC TGCAAGGCTT TTTACTGGTA GGAAGAACAA GATTAAACTT 
GGATCCGAGA CACTAATGTT TACACGAAAA CGTTTCATGG GTGATTTGGT TACAAGCGCG 
TTGCAAAGTT ATCAGTTTAG TAAGATATGT GCTTCAAAGA CTTCCATCGA GCTGAGAGAG 
GCATTGTCTA GTAGAAGAGC TGAGGCTGAT GACCTGAAGA AGGTAACATC ATATTCATTC 
AGGACAAAAG CAGGTGCTCT GGTTAAAGTC AAAGTTGAAA AGAAGAGAGA AAAGTACAGC 
ATTTTGGTTT ATGTCTCATC TTTGGAACTT AGTGGTGATG ACAAGAGTAG GCTGGTGATG 
GTTTGGGGTG TCTACAGGTC TGATTCTTCG TGTTTTTTGC CTCTGGATTT CGAAAACTCT 
TCCCAAGACT CACAAACCCA CACAACAGAA ACTACATTTG TGAAAAGCTC CTTGTCTGAG 
TTAATGCTAG GGCTAGAATT TGATGGGAAA GAATCTCCTT TCTACTTATC ATTTCACCTG 
AAGTTAGTGT CAGGTAGAGA TCCGGACGGT CAAGAAATGC TGACTCACAG GGACACTGAC 
TTCTGTATCC CGGTTGGTTT TACTGCTGGT CATCCATTGC CGCTAGGCCT TTCTTCCGGG 
CCAGATGATG ATTCTTGGAA TTTTTCGTTC TTTTCAAGAA GCTCAACGAA CGTGGTTTTA 
TGCTTGTATG ATGATAGCAC AACAGATAAA CCTGCTTTAG AACTTGATCT TGATCCTTAT 
GTCAACCGAA CAGGTGATGT TTGGCATGCT TCAGTTGACA ATACATGGGA TTTTGTGAGA 
TATGGCTACC GTTGCAAGGA GACAGCTCAC TCTAAAGAAG ATGTCGATGT TGAAGGTGAG 
CCAATTGTCT TGGATCCATA TGCCACAGTC GTTGGTAAAT CTGTCTCTCA GAAATATCTT 
GGAAGTTTAT CTAAGAGTCC TTCTTTTGAT TGGGGAGAAG ATGTGTCTCC GAACATACCT 
CTGGAGAAAC TTCTTGTTTA CCGGTTGAAT GTGAAGGGTT TTACACAACA CAGATCCAGC 
AAGTTGCCTA GTAACGTAGC AGGGACATTT TCTGGTGTAG CTGAGAAAGT TAGCCATTTG 
AAAACCCTTG GAACCAACGC TGTTCTTTTG GAGCCAATAT TCTCATTCTC CGAGCAAAAA 
GGTCCTTATT TTCCATTTCA TTTCTTTTCA CCAATGGACA TATATGGACC TTCCAATAGC 
CTTGAATCCG CAGTTAACTC AATGAAAGTG ATGGTGAAGA AATTGCACAG TGAGGGAATA 
GAGGTACTTT TGGAAGTAGT TTTTACACAT ACTGCTGATT CTGGAGCTCT TCGTGGAATT 
GATGACAGTT CCTATTACTA CAAGGGAAGA GCCAATGATC TAGATTCTAA AAGTTACTTG 
AACTGTAACT ATCCTGTTGT TCAGCAGTTG GTATTGGAGA GCTTGCGTTA TTGGGTAACC 
GAGTTTCATG TAGATGGATT TTGTTTTATA AATGCTTCAT CTCTCTTGAG AGGCGTTCAC 
GGTGAACAGC TCTCTCGTCC TCCTTTGGTT GAAGCAATAG CTTTTGATCC ACTTCTTGCG 
GAGACCAAAC TAATAGCTGA TTGCTGGGAT CCACTTGAAA TGATGCCAAA AGAAGTACGG 
TTCCCACATT GGAAGCGATG GGCAGAACTC AACACAAGAT ATTGTCGAAA TGTAAGAAAT 
TTTTTAAGGG GAAGAGGCGT TCTTAGTGAT CTGGCTACAA GAATTTGTGG AAGTGGTGAC 
GTATTTACCG ATGGAAGAGG TCCGGCCTTC TCCTTCAACT ACATTTCAAG AAACTCAGGA 
CTCTCGCTTG TTGACATAGT CAGTTTCAGT GGCCCTGAGC TAGCTTCAGA GCTAAGTTGG 
AATTGTGGGG AAGAAGGAGC AACGAATAAA TCAGCTGTTC TTCAAAGAAG GCTAAAACAA 
ATCCGCAATT TCTTGTTTAT ACAGTACATA TCACTTGGAG TTCCAGTGCT TAATATGGGA 
GATGAATGTG GAATCTCGAC AAGGGGCTCG CCTTTGTTGG AATCACGGAA ACCTTTTGAT 
TGGAACTTGC TAGCTTCGGC TTTCGGTACA CAGATCACGC AATTTATCTC CTTCATGACT 
TCAGTAAGAG CAAGAAGAAG TGATGTGTTT CAAAGGAGAG ACTTTTTGAA ACCTGAAAAT 
ATTGTTTGGT ATGCAAATGA CCAAACAACT CCAAAGTGGG AAGATCCTGC TTCTAAGTTT 
TTGGCATTGG AGATCAAATC AGAGTCAGAG GAAGAGGAAA CTGCGTCATT GGCTGAGCCA 
AATGAGCCAA AGAGCAATGA TCTGTTCATC GGATTCAATG CGAGTGATCA TCCTGAGAGT 
GTAGTCTTAC CTTCACTTCC CGATGGAAGC AAATGGAGAA GATTGGTCGA CACAGCTCTT 
CCTTTCCCGG GGTTTTTCTC TGTCGAAGGA GAAACCGTTG TAGCAGAAGA ACCGTTGCAG 
CAACTGGTTG TGTATGAGAT GAAGCCGTAC AGTTGTACCC TTTTTGAAAC CATCAATACT 
ACCGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DBE1 ( NM_100213.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_171830.1 Arabidopsis thaliana debranching enzyme 1 (DBE1), mRNA.

Price & Availability↑

CloneID	OAb24853
Clone ID Related Accession (Same CDS sequence)	NM_202022.3 , NM_100213.4
Accession Version	NM_100213.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2649bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	debranching enzyme 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_100213.3.

ATGGCTGCGT GGTCACCATC AGTTGGGATA GGATCTTGTT GTCTCAATAA TGGCATCACT 
AGAACATGGA AGTTTCCTTC TGCAAGGCTT TTTACTGGTA GGAAGAACAA GATTAAACTT 
GGATCCGAGA CACTAATGTT TACACGAAAA CGTTTCATGG GTGATTTGGT TACAAGCGCG 
TTGCAAAGTT ATCAGTTTAG TAAGATATGT GCTTCAAAGA CTTCCATCGA GCTGAGAGAG 
GCATTGTCTA GTAGAAGAGC TGAGGCTGAT GACCTGAAGA AGGTAACATC ATATTCATTC 
AGGACAAAAG CAGGTGCTCT GGTTAAAGTC AAAGTTGAAA AGAAGAGAGA AAAGTACAGC 
ATTTTGGTTT ATGTCTCATC TTTGGAACTT AGTGGTGATG ACAAGAGTAG GCTGGTGATG 
GTTTGGGGTG TCTACAGGTC TGATTCTTCG TGTTTTTTGC CTCTGGATTT CGAAAACTCT 
TCCCAAGACT CACAAACCCA CACAACAGAA ACTACATTTG TGAAAAGCTC CTTGTCTGAG 
TTAATGCTAG GGCTAGAATT TGATGGGAAA GAATCTCCTT TCTACTTATC ATTTCACCTG 
AAGTTAGTGT CAGGTAGAGA TCCGGACGGT CAAGAAATGC TGACTCACAG GGACACTGAC 
TTCTGTATCC CGGTTGGTTT TACTGCTGGT CATCCATTGC CGCTAGGCCT TTCTTCCGGG 
CCAGATGATG ATTCTTGGAA TTTTTCGTTC TTTTCAAGAA GCTCAACGAA CGTGGTTTTA 
TGCTTGTATG ATGATAGCAC AACAGATAAA CCTGCTTTAG AACTTGATCT TGATCCTTAT 
GTCAACCGAA CAGGTGATGT TTGGCATGCT TCAGTTGACA ATACATGGGA TTTTGTGAGA 
TATGGCTACC GTTGCAAGGA GACAGCTCAC TCTAAAGAAG ATGTCGATGT TGAAGGTGAG 
CCAATTGTCT TGGATCCATA TGCCACAGTC GTTGGTAAAT CTGTCTCTCA GAAATATCTT 
GGAAGTTTAT CTAAGAGTCC TTCTTTTGAT TGGGGAGAAG ATGTGTCTCC GAACATACCT 
CTGGAGAAAC TTCTTGTTTA CCGGTTGAAT GTGAAGGGTT TTACACAACA CAGATCCAGC 
AAGTTGCCTA GTAACGTAGC AGGGACATTT TCTGGTGTAG CTGAGAAAGT TAGCCATTTG 
AAAACCCTTG GAACCAACGC TGTTCTTTTG GAGCCAATAT TCTCATTCTC CGAGCAAAAA 
GGTCCTTATT TTCCATTTCA TTTCTTTTCA CCAATGGACA TATATGGACC TTCCAATAGC 
CTTGAATCCG CAGTTAACTC AATGAAAGTG ATGGTGAAGA AATTGCACAG TGAGGGAATA 
GAGGTACTTT TGGAAGTAGT TTTTACACAT ACTGCTGATT CTGGAGCTCT TCGTGGAATT 
GATGACAGTT CCTATTACTA CAAGGGAAGA GCCAATGATC TAGATTCTAA AAGTTACTTG 
AACTGTAACT ATCCTGTTGT TCAGCAGTTG GTATTGGAGA GCTTGCGTTA TTGGGTAACC 
GAGTTTCATG TAGATGGATT TTGTTTTATA AATGCTTCAT CTCTCTTGAG AGGCGTTCAC 
GGTGAACAGC TCTCTCGTCC TCCTTTGGTT GAAGCAATAG CTTTTGATCC ACTTCTTGCG 
GAGACCAAAC TAATAGCTGA TTGCTGGGAT CCACTTGAAA TGATGCCAAA AGAAGTACGG 
TTCCCACATT GGAAGCGATG GGCAGAACTC AACACAAGAT ATTGTCGAAA TGTAAGAAAT 
TTTTTAAGGG GAAGAGGCGT TCTTAGTGAT CTGGCTACAA GAATTTGTGG AAGTGGTGAC 
GTATTTACCG ATGGAAGAGG TCCGGCCTTC TCCTTCAACT ACATTTCAAG AAACTCAGGA 
CTCTCGCTTG TTGACATAGT CAGTTTCAGT GGCCCTGAGC TAGCTTCAGA GCTAAGTTGG 
AATTGTGGGG AAGAAGGAGC AACGAATAAA TCAGCTGTTC TTCAAAGAAG GCTAAAACAA 
ATCCGCAATT TCTTGTTTAT ACAGTACATA TCACTTGGAG TTCCAGTGCT TAATATGGGA 
GATGAATGTG GAATCTCGAC AAGGGGCTCG CCTTTGTTGG AATCACGGAA ACCTTTTGAT 
TGGAACTTGC TAGCTTCGGC TTTCGGTACA CAGATCACGC AATTTATCTC CTTCATGACT 
TCAGTAAGAG CAAGAAGAAG TGATGTGTTT CAAAGGAGAG ACTTTTTGAA ACCTGAAAAT 
ATTGTTTGGT ATGCAAATGA CCAAACAACT CCAAAGTGGG AAGATCCTGC TTCTAAGTTT 
TTGGCATTGG AGATCAAATC AGAGTCAGAG GAAGAGGAAA CTGCGTCATT GGCTGAGCCA 
AATGAGCCAA AGAGCAATGA TCTGTTCATC GGATTCAATG CGAGTGATCA TCCTGAGAGT 
GTAGTCTTAC CTTCACTTCC CGATGGAAGC AAATGGAGAA GATTGGTCGA CACAGCTCTT 
CCTTTCCCGG GGTTTTTCTC TGTCGAAGGA GAAACCGTTG TAGCAGAAGA ACCGTTGCAG 
CAACTGGTTG TGTATGAGAT GAAGCCGTAC AGTTGTACCC TTTTTGAAAC CATCAATACT 
ACCGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_171830.1
CDS	216..2864
Translation	MAAWSPSVGIGSCCLNNGITRTWKFPSARLFTGRKNKIKLGSETLMFTRKRFMGDLVTSALQSYQFSKICASKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSILVYVSSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELMLGLEFDGKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVVGKSVSQKYLGSLSKSPSFDWGEDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFSFSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSGALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYCRNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGPELASELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLESRKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWEDPASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRLVDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFETINTTA

Target ORF information:

RefSeq Version	NM_100213.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana debranching enzyme 1 (DBE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_100213.4

ORF Insert Sequence:

ATGGCTGCGT GGTCACCATC AGTTGGGATA GGATCTTGTT GTCTCAATAA TGGCATCACT 
AGAACATGGA AGTTTCCTTC TGCAAGGCTT TTTACTGGTA GGAAGAACAA GATTAAACTT 
GGATCCGAGA CACTAATGTT TACACGAAAA CGTTTCATGG GTGATTTGGT TACAAGCGCG 
TTGCAAAGTT ATCAGTTTAG TAAGATATGT GCTTCAAAGA CTTCCATCGA GCTGAGAGAG 
GCATTGTCTA GTAGAAGAGC TGAGGCTGAT GACCTGAAGA AGGTAACATC ATATTCATTC 
AGGACAAAAG CAGGTGCTCT GGTTAAAGTC AAAGTTGAAA AGAAGAGAGA AAAGTACAGC 
ATTTTGGTTT ATGTCTCATC TTTGGAACTT AGTGGTGATG ACAAGAGTAG GCTGGTGATG 
GTTTGGGGTG TCTACAGGTC TGATTCTTCG TGTTTTTTGC CTCTGGATTT CGAAAACTCT 
TCCCAAGACT CACAAACCCA CACAACAGAA ACTACATTTG TGAAAAGCTC CTTGTCTGAG 
TTAATGCTAG GGCTAGAATT TGATGGGAAA GAATCTCCTT TCTACTTATC ATTTCACCTG 
AAGTTAGTGT CAGGTAGAGA TCCGGACGGT CAAGAAATGC TGACTCACAG GGACACTGAC 
TTCTGTATCC CGGTTGGTTT TACTGCTGGT CATCCATTGC CGCTAGGCCT TTCTTCCGGG 
CCAGATGATG ATTCTTGGAA TTTTTCGTTC TTTTCAAGAA GCTCAACGAA CGTGGTTTTA 
TGCTTGTATG ATGATAGCAC AACAGATAAA CCTGCTTTAG AACTTGATCT TGATCCTTAT 
GTCAACCGAA CAGGTGATGT TTGGCATGCT TCAGTTGACA ATACATGGGA TTTTGTGAGA 
TATGGCTACC GTTGCAAGGA GACAGCTCAC TCTAAAGAAG ATGTCGATGT TGAAGGTGAG 
CCAATTGTCT TGGATCCATA TGCCACAGTC GTTGGTAAAT CTGTCTCTCA GAAATATCTT 
GGAAGTTTAT CTAAGAGTCC TTCTTTTGAT TGGGGAGAAG ATGTGTCTCC GAACATACCT 
CTGGAGAAAC TTCTTGTTTA CCGGTTGAAT GTGAAGGGTT TTACACAACA CAGATCCAGC 
AAGTTGCCTA GTAACGTAGC AGGGACATTT TCTGGTGTAG CTGAGAAAGT TAGCCATTTG 
AAAACCCTTG GAACCAACGC TGTTCTTTTG GAGCCAATAT TCTCATTCTC CGAGCAAAAA 
GGTCCTTATT TTCCATTTCA TTTCTTTTCA CCAATGGACA TATATGGACC TTCCAATAGC 
CTTGAATCCG CAGTTAACTC AATGAAAGTG ATGGTGAAGA AATTGCACAG TGAGGGAATA 
GAGGTACTTT TGGAAGTAGT TTTTACACAT ACTGCTGATT CTGGAGCTCT TCGTGGAATT 
GATGACAGTT CCTATTACTA CAAGGGAAGA GCCAATGATC TAGATTCTAA AAGTTACTTG 
AACTGTAACT ATCCTGTTGT TCAGCAGTTG GTATTGGAGA GCTTGCGTTA TTGGGTAACC 
GAGTTTCATG TAGATGGATT TTGTTTTATA AATGCTTCAT CTCTCTTGAG AGGCGTTCAC 
GGTGAACAGC TCTCTCGTCC TCCTTTGGTT GAAGCAATAG CTTTTGATCC ACTTCTTGCG 
GAGACCAAAC TAATAGCTGA TTGCTGGGAT CCACTTGAAA TGATGCCAAA AGAAGTACGG 
TTCCCACATT GGAAGCGATG GGCAGAACTC AACACAAGAT ATTGTCGAAA TGTAAGAAAT 
TTTTTAAGGG GAAGAGGCGT TCTTAGTGAT CTGGCTACAA GAATTTGTGG AAGTGGTGAC 
GTATTTACCG ATGGAAGAGG TCCGGCCTTC TCCTTCAACT ACATTTCAAG AAACTCAGGA 
CTCTCGCTTG TTGACATAGT CAGTTTCAGT GGCCCTGAGC TAGCTTCAGA GCTAAGTTGG 
AATTGTGGGG AAGAAGGAGC AACGAATAAA TCAGCTGTTC TTCAAAGAAG GCTAAAACAA 
ATCCGCAATT TCTTGTTTAT ACAGTACATA TCACTTGGAG TTCCAGTGCT TAATATGGGA 
GATGAATGTG GAATCTCGAC AAGGGGCTCG CCTTTGTTGG AATCACGGAA ACCTTTTGAT 
TGGAACTTGC TAGCTTCGGC TTTCGGTACA CAGATCACGC AATTTATCTC CTTCATGACT 
TCAGTAAGAG CAAGAAGAAG TGATGTGTTT CAAAGGAGAG ACTTTTTGAA ACCTGAAAAT 
ATTGTTTGGT ATGCAAATGA CCAAACAACT CCAAAGTGGG AAGATCCTGC TTCTAAGTTT 
TTGGCATTGG AGATCAAATC AGAGTCAGAG GAAGAGGAAA CTGCGTCATT GGCTGAGCCA 
AATGAGCCAA AGAGCAATGA TCTGTTCATC GGATTCAATG CGAGTGATCA TCCTGAGAGT 
GTAGTCTTAC CTTCACTTCC CGATGGAAGC AAATGGAGAA GATTGGTCGA CACAGCTCTT 
CCTTTCCCGG GGTTTTTCTC TGTCGAAGGA GAAACCGTTG TAGCAGAAGA ACCGTTGCAG 
CAACTGGTTG TGTATGAGAT GAAGCCGTAC AGTTGTACCC TTTTTGAAAC CATCAATACT 
ACCGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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DBE1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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