AT1G04650 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AT1G04650
Entrez Gene ID	839457
Full Name	holliday junction resolvase
Synonyms	T1G11.9,T1G11_9
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001331492.1	NP_001323014.1	holliday junction resolvase
NM_100345.3	NP_171959.2	holliday junction resolvase

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	AT1G04650	NP_171959.2
Oryza sativa (rice)	Os03g0144000	NP_001048942.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AT1G04650 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT1G04650 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb03676	NM_100345.3 Latest version!	Arabidopsis thaliana holliday junction resolvase (AT1G04650), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb33156	NM_001331492.1 Latest version!	Arabidopsis thaliana holliday junction resolvase (AT1G04650), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AT1G04650 ( NM_100345.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_171959.2 Arabidopsis thaliana holliday junction resolvase (AT1G04650), mRNA.

Price & Availability↑

CloneID	OAb03676
Clone ID Related Accession (Same CDS sequence)	NM_100345.3
Accession Version	NM_100345.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	holliday junction resolvase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_100345.2.

ATGGAGAAGA ATGCGATGAA GTTACTCGAA GAGATCAAAT CTTCTGATCT TATCGAGAAT 
CGGGCTCAAT TACTCACGCG GCTCAGTCAG TTAGATGTTG AAGAGAATTC TGATTTGCCT 
TCTTTTGTGG AGAGCCTAAC TACACTCTGG GAAGATTTCA CATGTCTTGA CGTTTCCCTG 
TGTTTGTTGA ACAAGGCGAT TCTGCCTGTG GCTTCAAAAT ACCTCGCTTT GGATCGGCCT 
GATTGCTCAC ACTACTTTCT TGCTTTTGCT ATTAAGGTAA GCCAATGGTG TGCCAAACAT 
TTAAACATGT CTGTTATGTC CATGGAGGAG TCTCAAGAGG AAGAGCATTC CAACATTTTC 
TTTCAGCTTC TTTTGGATTA TCTTCGTTTT TCTGCCTCAA GCTTTACTGC TATTGGAAAA 
ACTTGTTTCA TGACTGATGA TGCCTCAGCT GTTACAGTTC ATAAGTTTGT TTCAGAGCAG 
CTCAATTTGA CAAAGGAGTT GATAATGAAT TCCAAGAAAG TCGAATCCTT TTCCTCGGAG 
ATTTTTAAGG CTGTGCAAGT GGTAATTGAT TCTACTGTTC GTCTGTGCAA AGAATACTCT 
CAAACAGTGA ATCGTGAGGT AAGTGAAATG AAGACGAGTG GACATGTAGG AAAGGCAAGG 
ATGGAGGAAG GCAACGCTGT GGGTAATTTG GTGAGCATGA TTACGCTGGG AGTTAAATCT 
CTGAGTGAAT TGGGTATGCT TGCTGCAAGA GATGGTGGAA ATCTTGTGGC CATTCTTAAT 
ACATCTTGGA AGGGTGTGAT TACATTGCTT CAACTAGACA AGCAGACATT GGTATCTAAG 
GTAGATGTAG GAGAAATTAT CTTAAAGCTA ATATCACTGA TCAAGGACTC CCTGAGATTT 
GCTGCTGAGG CTTGGTCTTG CTCGGTGAAG GAAAACATAT CTGCAACGGA AGCCAGAAGG 
GTCTTTCTTC CTGTGAAATT TTATCTTATC AATGCTGTTA AAGTTGTGGC GCTGTTTCCA 
AGCCAGGCGT CTATGGTGTC CAAGGATATA GCACTATGCA TCTTGATGAT CTCTGCTTTC 
AAAGTTTCAC TGAGCCAACA AACTCATGGA AAATCCGCAA GTGAAGTAAT GACTGATCTT 
CTGGAGAAAA CAACTGTAGA TCTTCTAGGT GCACTGTTAA ATGCAGCTGA ACTAACACAG 
GAATTCAGGC TCACACTTCT TGATTCATTG TTCGTTGATG AATTCTCAAA CCAAATCTGT 
AAGAAGCAGA GCCATGATTC TCACACAAAA ACATCATTGG TGGATATCTT GTCTTTATCT 
GTTGAATCTG CGACAAGTGC TAGAGATTTG CTCCTGGCCC GAGTTGTATT GTTCCAGTCT 
GTCATGAGGT ATTCTTTCGA GCTTGACAAA GATGCAAAGC TTGCAATCAC CACAAAGCTG 
CAATGGCTTC TCGACATATT AGCAGACAAA GAGGTTTACT CTTCAGTTCT TTCCTCTCAG 
CTACCAATGG CAGATGGTTC TGGGAAGATA GTAATCTGGG AATCAATGTA TTCAGCTCTG 
CTTCTGTCTC TCAAAACCCT TATGATCATA CTCTCTTCAA CTCCTGCCTG GGAAGAGCTT 
GAAACATTCC TACTTCAGAA TCTCTTGCAT CCTCACTTCT TGTGTTGGCA GATAGTCATG 
GAACTCTGGT GCTTCTGGGT TCGTCATGCC ACTGACGATT TGGTGGTCGA TATGATTAAT 
CAACTTTGTA CATTCATTAT GTCAATGCCA TCATCAGAAA CACCTCTTTG TCCAGATTCC 
GTTCTCAGAA GAACTACAAA GTCCATCTGC TTTCTTCTTA CCCATAGCCC CAAATCTTTA 
ACAGTTCAAG TTTACAAACA TATTTCTACT GAGAGCAGAT CTGACCATGC ACCTGATGTA 
TATCTAGCAT TGCTGTTAGA CGGGTTTCCA CTGAATTTTC TTCCAGACAG AATAAAAAAC 
GACGCAAAGA GACAGATATT TGCAGATTTC TTCAACTTCA TCGAGAAATT CGATGAGAAA 
CCGTCAAATT CCTCCAGATA CACTCTGCTT GGAGCTCCAG TTTTCACAGT ATCTGCTTGC 
CTTCGGATAC TAAAGATGAG CATATCAGAG ATTGATGCCA AAACTCTGAA CTTTGTCGTC 
GCTCTCATTC AAAAGTACAG AAACTCCAAA GATGAAACGA CGAAGGAACG TTACAGTGAG 
ATTCTCAGCG AAACATTATC TATCATCTCA AGAAGCGAAC AGCTATACAC TTGCCAAGAG 
ATGGATAATG TCATAACAGA GCTTCAAAAG CTATTCAATT CAGAAACTAA TCATCATCAT 
AATCATCTTC GTAAGTCCAA GCCAAACCTT GCTCTCTTCT TGTCAGGACT CAGCAAATAC 
GAGATGTCTG AAACCAAAAA GTGTCCAAAG AGCATTGCGG TTTGGGAACT CTACCATATG 
CTTCTCAGGA AACGCCACTG GGCTTTGGTA CACCACGCGG TTACTGCGTT TGGATACTTC 
TGTGCACGTA CAAGCTGTAA TCAGCTATGG AGATTCGTAC CTGAAGACGC GGCTCTAGCC 
TTCGATATAG CTTCTGGAAA AGAAGCGAAA ACAGAGCGGT TCATGTCGGA ATTAAAGATG 
TTTTTGGAGA AAGAACAAGC GCTTCTTTCA ATCACACCTT CAGAAGAAGA ACTGGAGTTA 
CTTTCGAAAG AAGGCACCGA GGTTAAGGCA ACAGTGCAGA AGCTTCTAGA AGGAAGAAGC 
CAGAGGTCAA TGGAAGTAGA GAAACGACCG AACAAGAAAA GGAAACTTCC AGAAGGAATT 
TGCAGAGGAA TGGAGTTATT GCAGAATGGA GTGAAGAGGA TTAACGAGGG TTTAAACGAA 
TTGAGGTCAG ATGAAAATGA GAGTGAAGAG TTTCAAAAGA GTTTATCGAA TCAGTTTTCG 
TGTCTTGAAG ATTTGGTCTC TCATTTGTTA AGCCTCACCG CAGCTTCGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_171959.2
CDS	121..3114
Translation	MEKNAMKLLEEIKSSDLIENRAQLLTRLSQLDVEENSDLPSFVESLTTLWEDFTCLDVSLCLLNKAILPVASKYLALDRPDCSHYFLAFAIKVSQWCAKHLNMSVMSMEESQEEEHSNIFFQLLLDYLRFSASSFTAIGKTCFMTDDASAVTVHKFVSEQLNLTKELIMNSKKVESFSSEIFKAVQVVIDSTVRLCKEYSQTVNREVSEMKTSGHVGKARMEEGNAVGNLVSMITLGVKSLSELGMLAARDGGNLVAILNTSWKGVITLLQLDKQTLVSKVDVGEIILKLISLIKDSLRFAAEAWSCSVKENISATEARRVFLPVKFYLINAVKVVALFPSQASMVSKDIALCILMISAFKVSLSQQTHGKSASEVMTDLLEKTTVDLLGALLNAAELTQEFRLTLLDSLFVDEFSNQICKKQSHDSHTKTSLVDILSLSVESATSARDLLLARVVLFQSVMRYSFELDKDAKLAITTKLQWLLDILADKEVYSSVLSSQLPMADGSGKIVIWESMYSALLLSLKTLMIILSSTPAWEELETFLLQNLLHPHFLCWQIVMELWCFWVRHATDDLVVDMINQLCTFIMSMPSSETPLCPDSVLRRTTKSICFLLTHSPKSLTVQVYKHISTESRSDHAPDVYLALLLDGFPLNFLPDRIKNDAKRQIFADFFNFIEKFDEKPSNSSRYTLLGAPVFTVSACLRILKMSISEIDAKTLNFVVALIQKYRNSKDETTKERYSEILSETLSIISRSEQLYTCQEMDNVITELQKLFNSETNHHHNHLRKSKPNLALFLSGLSKYEMSETKKCPKSIAVWELYHMLLRKRHWALVHHAVTAFGYFCARTSCNQLWRFVPEDAALAFDIASGKEAKTERFMSELKMFLEKEQALLSITPSEEELELLSKEGTEVKATVQKLLEGRSQRSMEVEKRPNKKRKLPEGICRGMELLQNGVKRINEGLNELRSDENESEEFQKSLSNQFSCLEDLVSHLLSLTAASD

Target ORF information:

RefSeq Version	NM_100345.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana holliday junction resolvase (AT1G04650), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_100345.3

ORF Insert Sequence:

ATGGAGAAGA ATGCGATGAA GTTACTCGAA GAGATCAAAT CTTCTGATCT TATCGAGAAT 
CGGGCTCAAT TACTCACGCG GCTCAGTCAG TTAGATGTTG AAGAGAATTC TGATTTGCCT 
TCTTTTGTGG AGAGCCTAAC TACACTCTGG GAAGATTTCA CATGTCTTGA CGTTTCCCTG 
TGTTTGTTGA ACAAGGCGAT TCTGCCTGTG GCTTCAAAAT ACCTCGCTTT GGATCGGCCT 
GATTGCTCAC ACTACTTTCT TGCTTTTGCT ATTAAGGTAA GCCAATGGTG TGCCAAACAT 
TTAAACATGT CTGTTATGTC CATGGAGGAG TCTCAAGAGG AAGAGCATTC CAACATTTTC 
TTTCAGCTTC TTTTGGATTA TCTTCGTTTT TCTGCCTCAA GCTTTACTGC TATTGGAAAA 
ACTTGTTTCA TGACTGATGA TGCCTCAGCT GTTACAGTTC ATAAGTTTGT TTCAGAGCAG 
CTCAATTTGA CAAAGGAGTT GATAATGAAT TCCAAGAAAG TCGAATCCTT TTCCTCGGAG 
ATTTTTAAGG CTGTGCAAGT GGTAATTGAT TCTACTGTTC GTCTGTGCAA AGAATACTCT 
CAAACAGTGA ATCGTGAGGT AAGTGAAATG AAGACGAGTG GACATGTAGG AAAGGCAAGG 
ATGGAGGAAG GCAACGCTGT GGGTAATTTG GTGAGCATGA TTACGCTGGG AGTTAAATCT 
CTGAGTGAAT TGGGTATGCT TGCTGCAAGA GATGGTGGAA ATCTTGTGGC CATTCTTAAT 
ACATCTTGGA AGGGTGTGAT TACATTGCTT CAACTAGACA AGCAGACATT GGTATCTAAG 
GTAGATGTAG GAGAAATTAT CTTAAAGCTA ATATCACTGA TCAAGGACTC CCTGAGATTT 
GCTGCTGAGG CTTGGTCTTG CTCGGTGAAG GAAAACATAT CTGCAACGGA AGCCAGAAGG 
GTCTTTCTTC CTGTGAAATT TTATCTTATC AATGCTGTTA AAGTTGTGGC GCTGTTTCCA 
AGCCAGGCGT CTATGGTGTC CAAGGATATA GCACTATGCA TCTTGATGAT CTCTGCTTTC 
AAAGTTTCAC TGAGCCAACA AACTCATGGA AAATCCGCAA GTGAAGTAAT GACTGATCTT 
CTGGAGAAAA CAACTGTAGA TCTTCTAGGT GCACTGTTAA ATGCAGCTGA ACTAACACAG 
GAATTCAGGC TCACACTTCT TGATTCATTG TTCGTTGATG AATTCTCAAA CCAAATCTGT 
AAGAAGCAGA GCCATGATTC TCACACAAAA ACATCATTGG TGGATATCTT GTCTTTATCT 
GTTGAATCTG CGACAAGTGC TAGAGATTTG CTCCTGGCCC GAGTTGTATT GTTCCAGTCT 
GTCATGAGGT ATTCTTTCGA GCTTGACAAA GATGCAAAGC TTGCAATCAC CACAAAGCTG 
CAATGGCTTC TCGACATATT AGCAGACAAA GAGGTTTACT CTTCAGTTCT TTCCTCTCAG 
CTACCAATGG CAGATGGTTC TGGGAAGATA GTAATCTGGG AATCAATGTA TTCAGCTCTG 
CTTCTGTCTC TCAAAACCCT TATGATCATA CTCTCTTCAA CTCCTGCCTG GGAAGAGCTT 
GAAACATTCC TACTTCAGAA TCTCTTGCAT CCTCACTTCT TGTGTTGGCA GATAGTCATG 
GAACTCTGGT GCTTCTGGGT TCGTCATGCC ACTGACGATT TGGTGGTCGA TATGATTAAT 
CAACTTTGTA CATTCATTAT GTCAATGCCA TCATCAGAAA CACCTCTTTG TCCAGATTCC 
GTTCTCAGAA GAACTACAAA GTCCATCTGC TTTCTTCTTA CCCATAGCCC CAAATCTTTA 
ACAGTTCAAG TTTACAAACA TATTTCTACT GAGAGCAGAT CTGACCATGC ACCTGATGTA 
TATCTAGCAT TGCTGTTAGA CGGGTTTCCA CTGAATTTTC TTCCAGACAG AATAAAAAAC 
GACGCAAAGA GACAGATATT TGCAGATTTC TTCAACTTCA TCGAGAAATT CGATGAGAAA 
CCGTCAAATT CCTCCAGATA CACTCTGCTT GGAGCTCCAG TTTTCACAGT ATCTGCTTGC 
CTTCGGATAC TAAAGATGAG CATATCAGAG ATTGATGCCA AAACTCTGAA CTTTGTCGTC 
GCTCTCATTC AAAAGTACAG AAACTCCAAA GATGAAACGA CGAAGGAACG TTACAGTGAG 
ATTCTCAGCG AAACATTATC TATCATCTCA AGAAGCGAAC AGCTATACAC TTGCCAAGAG 
ATGGATAATG TCATAACAGA GCTTCAAAAG CTATTCAATT CAGAAACTAA TCATCATCAT 
AATCATCTTC GTAAGTCCAA GCCAAACCTT GCTCTCTTCT TGTCAGGACT CAGCAAATAC 
GAGATGTCTG AAACCAAAAA GTGTCCAAAG AGCATTGCGG TTTGGGAACT CTACCATATG 
CTTCTCAGGA AACGCCACTG GGCTTTGGTA CACCACGCGG TTACTGCGTT TGGATACTTC 
TGTGCACGTA CAAGCTGTAA TCAGCTATGG AGATTCGTAC CTGAAGACGC GGCTCTAGCC 
TTCGATATAG CTTCTGGAAA AGAAGCGAAA ACAGAGCGGT TCATGTCGGA ATTAAAGATG 
TTTTTGGAGA AAGAACAAGC GCTTCTTTCA ATCACACCTT CAGAAGAAGA ACTGGAGTTA 
CTTTCGAAAG AAGGCACCGA GGTTAAGGCA ACAGTGCAGA AGCTTCTAGA AGGAAGAAGC 
CAGAGGTCAA TGGAAGTAGA GAAACGACCG AACAAGAAAA GGAAACTTCC AGAAGGAATT 
TGCAGAGGAA TGGAGTTATT GCAGAATGGA GTGAAGAGGA TTAACGAGGG TTTAAACGAA 
TTGAGGTCAG ATGAAAATGA GAGTGAAGAG TTTCAAAAGA GTTTATCGAA TCAGTTTTCG 
TGTCTTGAAG ATTTGGTCTC TCATTTGTTA AGCCTCACCG CAGCTTCGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AT1G04650 ( NM_001331492.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001323014.1 Arabidopsis thaliana holliday junction resolvase (AT1G04650), partial mRNA.

Price & Availability↑

CloneID	OAb33156
Clone ID Related Accession (Same CDS sequence)	NM_001331492.1
Accession Version	NM_001331492.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	holliday junction resolvase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). COMPLETENESS: incomplete on the 3' end.

ATGTCTGTTA TGTCCATGGA GGAGTCTCAA GAGGAAGAGC ATTCCAACAT TTTCTTTCAG 
CTTCTTTTGG ATTATCTTCG TTTTTCTGCC TCAAGCTTTA CTGCTATTGG AAAAACTTGT 
TTCATGACTG ATGATGCCTC AGCTGTTACA GTTCATAAGT TTGTTTCAGA GCAGCTCAAT 
TTGACAAAGG AGTTGATAAT GAATTCCAAG AAAGTCGAAT CCTTTTCCTC GGAGATTTTT 
AAGGCTGTGC AAGTGGTAAT TGATTCTACT GTTCGTCTGT GCAAAGAATA CTCTCAAACA 
GTGAATCGTG AGGTAAGTGA AATGAAGACG AGTGGACATG TAGGAAAGGC AAGGATGGAG 
GAAGGCAACG CTGTGGGTAA TTTGGTGAGC ATGATTACGC TGGGAGTTAA ATCTCTGAGT 
GAATTGGGTA TGCTTGCTGC AAGAGATGGT GGAAATCTTG TGGCCATTCT TAATACATCT 
TGGAAGGGTG TGATTACATT GCTTCAACTA GACAAGCAGA CATTGGTATC TAAGGTAGAT 
GTAGGAGAAA TTATCTTAAA GCTAATATCA CTGATCAAGG ACTCCCTGAG ATTTGCTGCT 
GAGGCTTGGT CTTGCTCGGT GAAGGAAAAC ATATCTGCAA CGGAAGCCAG AAGGGTCTTT 
CTTCCTGTGA AATTTTATCT TATCAATGCT GTTAAAGTTG TGGCGCTGTT TCCAAGCCAG 
GCGTCTATGG TGTCCAAGGA TATAGCACTA TGCATCTTGA TGATCTCTGC TTTCAAAGTT 
TCACTGAGCC AACAAACTCA TGGAAAATCC GCAAGTGAAG TAATGACTGA TCTTCTGGAG 
AAAACAACTG TAGATCTTCT AGGTGCACTG TTAAATGCAG CTGAACTAAC ACAGGAATTC 
AGGCTCACAC TTCTTGATTC ATTGTTCGTT GATGAATTCT CAAACCAAAT CTGTAAGAAG 
CAGAGCCATG ATTCTCACAC AAAAACATCA TTGGTGGATA TCTTGTCTTT ATCTGTTGAA 
TCTGCGACAA GTGCTAGAGA TTTGCTCCTG GCCCGAGTTG TATTGTTCCA GTCTGTCATG 
AGGTATTCTT TCGAGCTTGA CAAAGATGCA AAGCTTGCAA TCACCACAAA GCTGCAATGG 
CTTCTCGACA TATTAGCAGA CAAAGAGGTT TACTCTTCAG TTCTTTCCTC TCAGCTACCA 
ATGGCAGATG GTTCTGGGAA GATAGTAATC TGGGAATCAA TGTATTCAGC TCTGCTTCTG 
TCTCTCAAAA CCCTTATGAT CATACTCTCT TCAACTCCTG CCTGGGAAGA GCTTGAAACA 
TTCCTACTTC AGAATCTCTT GCATCCTCAC TTCTTGTGTT GGCAGATAGT CATGGAACTC 
TGGTGCTTCT GGGTTCGTCA TGCCACTGAC GATTTGGTGG TCGATATGAT TAATCAACTT 
TGTACATTCA TTATGTCAAT GCCATCATCA GAAACACCTC TTTGTCCAGA TTCCGTTCTC 
AGAAGAACTA CAAAGTCCAT CTGCTTTCTT CTTACCCATA GCCCCAAATC TTTAACAGTT 
CAAGTTTACA AACATATTTC TACTGAGAGC AGATCTGACC ATGCACCTGA TGTATATCTA 
GCATTGCTGT TAGACGGGTT TCCACTGAAT TTTCTTCCAG ACAGAATAAA AAACGACGCA 
AAGAGACAGA TATTTGCAGA TTTCTTCAAC TTCATCGAGA AATTCGATGA GAAACCGTCA 
AATTCCTCCA GATACACTCT GCTTGGAGCT CCAGTTTTCA CAGTATCTGC TTGCCTTCGG 
ATACTAAAGA TGAGCATATC AGAGATTGAT GCCAAAACTC TGAACTTTGT CGTCGCTCTC 
ATTCAAAAGT ACAGAAACTC CAAAGATGAA ACGACGAAGG AACGTTACAG TGAGATTCTC 
AGCGAAACAT TATCTATCAT CTCAAGAAGC GAACAGCTAT ACACTTGCCA AGAGATGGAT 
AATGTCATAA CAGAGCTTCA AAAGCTATTC AATTCAGAAA CTAATCATCA TCATAATCAT 
CTTCGTAAGT CCAAGCCAAA CCTTGCTCTC TTCTTGTCAG GACTCAGCAA ATACGAGATG 
TCTGAAACCA AAAAGTGTCC AAAGAGCATT GCGGTTTGGG AACTCTACCA TATGCTTCTC 
AGGAAACGCC ACTGGGCTTT GGTACACCAC GCGGTTACTG CGTTTGGATA CTTCTGTGCA 
CGTACAAGCT GTAATCAGCT ATGGAGATTC GTACCTGAAG ACGCGGCTCT AGCCTTCGAT 
ATAGCTTCTG GAAAAGAAGC GAAAACAGAG CGGTTCATGT CGGAATTAAA GATGTTTTTG 
GAGAAAGAAC AAGCGCTTCT TTCAATCACA CCTTCAGAAG AAGAACTGGA GTTACTTTCG 
AAAGAAGGCA CCGAGGTTAA GGCAACAGTG CAGAAGCTTC TAGAAGGAAG AAGCCAGAGG 
TCAATGGAAG TAGAGAAACG ACCGAACAAG AAAAGGAAAC TTCCAGAAGG AATTTGCAGA 
GGAATGGAGT TATTGCAGAA TGGAGTGAAG AGGATTAACG AGGGTTTAAA CGAATTGAGG 
TCAGATGAAA ATGAGAGTGA AGAGTTTCAA AAGAGTTTAT CGAATCAGTT TTCGTGTCTT 
GAAGATTTGG TCTCTCATTT GTTAAGCCTC ACCGCAGCTT CGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001323014.1
CDS	80..2767
Translation	MSVMSMEESQEEEHSNIFFQLLLDYLRFSASSFTAIGKTCFMTDDASAVTVHKFVSEQLNLTKELIMNSKKVESFSSEIFKAVQVVIDSTVRLCKEYSQTVNREVSEMKTSGHVGKARMEEGNAVGNLVSMITLGVKSLSELGMLAARDGGNLVAILNTSWKGVITLLQLDKQTLVSKVDVGEIILKLISLIKDSLRFAAEAWSCSVKENISATEARRVFLPVKFYLINAVKVVALFPSQASMVSKDIALCILMISAFKVSLSQQTHGKSASEVMTDLLEKTTVDLLGALLNAAELTQEFRLTLLDSLFVDEFSNQICKKQSHDSHTKTSLVDILSLSVESATSARDLLLARVVLFQSVMRYSFELDKDAKLAITTKLQWLLDILADKEVYSSVLSSQLPMADGSGKIVIWESMYSALLLSLKTLMIILSSTPAWEELETFLLQNLLHPHFLCWQIVMELWCFWVRHATDDLVVDMINQLCTFIMSMPSSETPLCPDSVLRRTTKSICFLLTHSPKSLTVQVYKHISTESRSDHAPDVYLALLLDGFPLNFLPDRIKNDAKRQIFADFFNFIEKFDEKPSNSSRYTLLGAPVFTVSACLRILKMSISEIDAKTLNFVVALIQKYRNSKDETTKERYSEILSETLSIISRSEQLYTCQEMDNVITELQKLFNSETNHHHNHLRKSKPNLALFLSGLSKYEMSETKKCPKSIAVWELYHMLLRKRHWALVHHAVTAFGYFCARTSCNQLWRFVPEDAALAFDIASGKEAKTERFMSELKMFLEKEQALLSITPSEEELELLSKEGTEVKATVQKLLEGRSQRSMEVEKRPNKKRKLPEGICRGMELLQNGVKRINEGLNELRSDENESEEFQKSLSNQFSCLEDLVSHLLSLTAASD

Target ORF information:

RefSeq Version	NM_001331492.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana holliday junction resolvase (AT1G04650), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001331492.1

ORF Insert Sequence:

ATGTCTGTTA TGTCCATGGA GGAGTCTCAA GAGGAAGAGC ATTCCAACAT TTTCTTTCAG 
CTTCTTTTGG ATTATCTTCG TTTTTCTGCC TCAAGCTTTA CTGCTATTGG AAAAACTTGT 
TTCATGACTG ATGATGCCTC AGCTGTTACA GTTCATAAGT TTGTTTCAGA GCAGCTCAAT 
TTGACAAAGG AGTTGATAAT GAATTCCAAG AAAGTCGAAT CCTTTTCCTC GGAGATTTTT 
AAGGCTGTGC AAGTGGTAAT TGATTCTACT GTTCGTCTGT GCAAAGAATA CTCTCAAACA 
GTGAATCGTG AGGTAAGTGA AATGAAGACG AGTGGACATG TAGGAAAGGC AAGGATGGAG 
GAAGGCAACG CTGTGGGTAA TTTGGTGAGC ATGATTACGC TGGGAGTTAA ATCTCTGAGT 
GAATTGGGTA TGCTTGCTGC AAGAGATGGT GGAAATCTTG TGGCCATTCT TAATACATCT 
TGGAAGGGTG TGATTACATT GCTTCAACTA GACAAGCAGA CATTGGTATC TAAGGTAGAT 
GTAGGAGAAA TTATCTTAAA GCTAATATCA CTGATCAAGG ACTCCCTGAG ATTTGCTGCT 
GAGGCTTGGT CTTGCTCGGT GAAGGAAAAC ATATCTGCAA CGGAAGCCAG AAGGGTCTTT 
CTTCCTGTGA AATTTTATCT TATCAATGCT GTTAAAGTTG TGGCGCTGTT TCCAAGCCAG 
GCGTCTATGG TGTCCAAGGA TATAGCACTA TGCATCTTGA TGATCTCTGC TTTCAAAGTT 
TCACTGAGCC AACAAACTCA TGGAAAATCC GCAAGTGAAG TAATGACTGA TCTTCTGGAG 
AAAACAACTG TAGATCTTCT AGGTGCACTG TTAAATGCAG CTGAACTAAC ACAGGAATTC 
AGGCTCACAC TTCTTGATTC ATTGTTCGTT GATGAATTCT CAAACCAAAT CTGTAAGAAG 
CAGAGCCATG ATTCTCACAC AAAAACATCA TTGGTGGATA TCTTGTCTTT ATCTGTTGAA 
TCTGCGACAA GTGCTAGAGA TTTGCTCCTG GCCCGAGTTG TATTGTTCCA GTCTGTCATG 
AGGTATTCTT TCGAGCTTGA CAAAGATGCA AAGCTTGCAA TCACCACAAA GCTGCAATGG 
CTTCTCGACA TATTAGCAGA CAAAGAGGTT TACTCTTCAG TTCTTTCCTC TCAGCTACCA 
ATGGCAGATG GTTCTGGGAA GATAGTAATC TGGGAATCAA TGTATTCAGC TCTGCTTCTG 
TCTCTCAAAA CCCTTATGAT CATACTCTCT TCAACTCCTG CCTGGGAAGA GCTTGAAACA 
TTCCTACTTC AGAATCTCTT GCATCCTCAC TTCTTGTGTT GGCAGATAGT CATGGAACTC 
TGGTGCTTCT GGGTTCGTCA TGCCACTGAC GATTTGGTGG TCGATATGAT TAATCAACTT 
TGTACATTCA TTATGTCAAT GCCATCATCA GAAACACCTC TTTGTCCAGA TTCCGTTCTC 
AGAAGAACTA CAAAGTCCAT CTGCTTTCTT CTTACCCATA GCCCCAAATC TTTAACAGTT 
CAAGTTTACA AACATATTTC TACTGAGAGC AGATCTGACC ATGCACCTGA TGTATATCTA 
GCATTGCTGT TAGACGGGTT TCCACTGAAT TTTCTTCCAG ACAGAATAAA AAACGACGCA 
AAGAGACAGA TATTTGCAGA TTTCTTCAAC TTCATCGAGA AATTCGATGA GAAACCGTCA 
AATTCCTCCA GATACACTCT GCTTGGAGCT CCAGTTTTCA CAGTATCTGC TTGCCTTCGG 
ATACTAAAGA TGAGCATATC AGAGATTGAT GCCAAAACTC TGAACTTTGT CGTCGCTCTC 
ATTCAAAAGT ACAGAAACTC CAAAGATGAA ACGACGAAGG AACGTTACAG TGAGATTCTC 
AGCGAAACAT TATCTATCAT CTCAAGAAGC GAACAGCTAT ACACTTGCCA AGAGATGGAT 
AATGTCATAA CAGAGCTTCA AAAGCTATTC AATTCAGAAA CTAATCATCA TCATAATCAT 
CTTCGTAAGT CCAAGCCAAA CCTTGCTCTC TTCTTGTCAG GACTCAGCAA ATACGAGATG 
TCTGAAACCA AAAAGTGTCC AAAGAGCATT GCGGTTTGGG AACTCTACCA TATGCTTCTC 
AGGAAACGCC ACTGGGCTTT GGTACACCAC GCGGTTACTG CGTTTGGATA CTTCTGTGCA 
CGTACAAGCT GTAATCAGCT ATGGAGATTC GTACCTGAAG ACGCGGCTCT AGCCTTCGAT 
ATAGCTTCTG GAAAAGAAGC GAAAACAGAG CGGTTCATGT CGGAATTAAA GATGTTTTTG 
GAGAAAGAAC AAGCGCTTCT TTCAATCACA CCTTCAGAAG AAGAACTGGA GTTACTTTCG 
AAAGAAGGCA CCGAGGTTAA GGCAACAGTG CAGAAGCTTC TAGAAGGAAG AAGCCAGAGG 
TCAATGGAAG TAGAGAAACG ACCGAACAAG AAAAGGAAAC TTCCAGAAGG AATTTGCAGA 
GGAATGGAGT TATTGCAGAA TGGAGTGAAG AGGATTAACG AGGGTTTAAA CGAATTGAGG 
TCAGATGAAA ATGAGAGTGA AGAGTTTCAA AAGAGTTTAT CGAATCAGTT TTCGTGTCTT 
GAAGATTTGG TCTCTCATTT GTTAAGCCTC ACCGCAGCTT CGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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AT1G04650 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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