AVP1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AVP1
Entrez Gene ID	838138
Full Name	Inorganic H pyrophosphatase family protein
Synonyms	ARABIDOPSIS THALIANA V-PPASE 3,ATAVP3,AVP-3,AtAVP1,AtVHP1;1,F7H2.3,F7H2_3,FUGU 5,FUGU5,VACUOLAR H+ - PYROPHOSPHATASE AVP-3,VHP1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001084073.1	NP_001077542.1	Inorganic H pyrophosphatase family protein
NM_101437.5	NP_173021.1	Inorganic H pyrophosphatase family protein

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os02g0802500	NP_001048422.1
Oryza sativa (rice)	Os06g0178900	NP_001056966.1
Arabidopsis thaliana (thale cress)	AVP1	NP_173021.1
Oryza sativa (rice)	Os06g0644200	NP_001058184.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

AVP1 role in phloem loading and transport
Title: Constitutive and Companion Cell-Specific Overexpression of AVP1, Encoding a Proton-Pumping Pyrophosphatase, Enhances Biomass Accumulation, Phloem Loading, and Long-Distance Transport.

AVP1 localized at the plasma membrane of the sieve element-companion cell complexes functions as a synthase, and that this activity is critical for the maintenance of pyrophosphate homeostasis required for phloem function.
Title: Arabidopsis type I proton-pumping pyrophosphatase expresses strongly in phloem, where it is required for pyrophosphate metabolism and photosynthate partitioning.

AVP1DOX plants produced 25% more marketable ripened fruit per plant under P-deficient conditions compared with the controls. Further, under low phosphate conditions, they displayed increased phosphate transport from leaf (source) to fruit (sink).
Title: Over-expression of the Arabidopsis proton-pyrophosphatase AVP1 enhances transplant survival, root mass, and fruit development under limiting phosphorus conditions.

The expression of AVP1 increases the shoot biomass of barley in saline and nonsaline conditions. It is shown that the expression of AVP1 in transgenic barley improves the grain yield per plant of this cereal crop when grown in a high-salinity field.
Title: Expression of the Arabidopsis vacuolar H?-pyrophosphatase gene (AVP1) improves the shoot biomass of transgenic barley and increases grain yield in a saline field.

Results show that transgenic plants expressing higher levels of AVP1 transcripts in sugarcane are able to withstand salt and drought stress regimes.
Title: Genetic improvement of sugarcane for drought and salinity stress tolerance using Arabidopsis vacuolar pyrophosphatase (AVP1) gene.

The following AVP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AVP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb23660	NM_001084073.1 Latest version!	Arabidopsis thaliana Inorganic H pyrophosphatase family protein (AVP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OAb23661	NM_101437.5 Latest version!	Arabidopsis thaliana Inorganic H pyrophosphatase family protein (AVP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AVP1 ( NM_001084073.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001077542.1 Arabidopsis thaliana Inorganic H pyrophosphatase family protein (AVP1), mRNA.

Price & Availability↑

CloneID	OAb23660
Clone ID Related Accession (Same CDS sequence)	NM_001084073.1
Accession Version	NM_001084073.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Inorganic H pyrophosphatase family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGGTGGCGC CTGCTTTGTT ACCGGAGCTC TGGACGGAGA TCCTTGTACC GATTTGTGCG 
GTGATTGGTA TCGCCTTTTC GCTTTTCCAA TGGTACGTTG TATCTCGCGT GAAACTCACC 
TCTGACCTCG GCGCATCGTC TTCCGGTGGA GCTAACAATG GGAAGAATGG ATACGGTGAT 
TATCTAATCG AGGAAGAGGA AGGTGTTAAT GACCAGAGTG TTGTCGCTAA GTGCGCTGAG 
ATTCAGACTG CTATTTCCGA AGGTGCAACT TCATTCCTAT TCACGGAGTA CAAATATGTT 
GGTGTCTTCA TGATTTTCTT TGCTGCTGTT ATCTTTGTTT TCCTCGGCTC TGTTGAGGGA 
TTCAGCACTG ATAACAAGCC TTGTACTTAC GACACCACCA GAACCTGCAA GCCTGCATTG 
GCTACTGCAG CTTTCAGTAC CATTGCTTTC GTGCTTGGTG CTGTTACCTC TGTTCTATCT 
GGTTTCCTTG GGATGAAGAT TGCTACATAC GCTAATGCTA GGACCACTTT GGAGGCGAGG 
AAAGGTGTTG GAAAGGCGTT CATTGTTGCA TTCAGGTCTG GTGCTGTGAT GGGTTTCCTT 
CTTGCAGCGA GTGGTCTATT GGTGCTTTAC ATTACTATCA ATGTGTTCAA GATCTATTAC 
GGAGATGACT GGGAAGGTCT TTTTGAGGCT ATTACTGGTT ATGGTCTTGG TGGGTCTTCC 
ATGGCTCTCT TTGGCCGTGT TGGTGGTGGG ATCTACACTA AGGCTGCTGA TGTCGGCGCT 
GACCTTGTCG GTAAAATTGA GAGGAATATT CCAGAGGATG ATCCAAGAAA CCCAGCTGTC 
ATTGCTGATA ATGTCGGTGA CAATGTTGGT GACATTGCTG GTATGGGATC TGATCTCTTT 
GGATCATATG CTGAAGCATC ATGCGCTGCT CTTGTTGTTG CCTCGATCTC ATCTTTCGGA 
ATCAACCACG ACTTCACTGC CATGTGCTAC CCATTGCTCA TCAGTTCAAT GGGAATCTTG 
GTTTGTTTGA TCACAACTCT CTTTGCCACT GACTTCTTTG AGATTAAGCT TGTCAAGGAG 
ATTGAACCAG CATTGAAGAA CCAGCTCATT ATCTCAACTG TTATTATGAC TGTTGGTATT 
GCTATTGTGT CATGGGTTGG CTTACCGACC TCCTTTACCA TCTTCAACTT TGGAACACAA 
AAAGTTGTCA AGAACTGGCA GCTATTCCTT TGTGTTTGTG TTGGTCTTTG GGCTGGACTC 
ATTATTGGTT TCGTCACTGA GTACTACACT AGTAACGCCT ACAGCCCTGT GCAAGATGTT 
GCAGATTCAT GCAGAACTGG TGCAGCTACC AATGTTATCT TCGGCCTTGC TCTTGGTTAC 
AAATCCGTCA TTATTCCAAT CTTTGCTATT GCTATCAGTA TATTCGTTAG CTTCAGCTTT 
GCTGCTATGT ATGGTGTTGC TGTTGCTGCT CTTGGTATGC TCAGTACCAT TGCCACTGGT 
TTGGCAATTG ATGCTTATGG TCCCATCAGT GACAATGCTG GTGGTATTGC TGAAATGGCT 
GGAATGAGCC ACCGCATCCG TGAAAGAACT GATGCTCTTG ATGCCGCTGG AAACACCACT 
GCTGCTATTG GAAAGGGATT TGCCATTGGC TCTGCTGCCC TAGTCTCCTT GGCTCTCTTT 
GGTGCCTTTG TGAGCCGTGC AGGGATCCAC ACCGTAGATG TTTTGACCCC TAAAGTTATC 
ATTGGGCTCC TTGTTGGTGC CATGCTTCCT TACTGGTTCT CTGCCATGAC AATGAAGAGT 
GTGGGAAGTG CAGCTCTTAA GATGGTTGAA GAAGTTCGCA GGCAGTTCAA CACCATCCCT 
GGACTTATGG AAGGAACCGC AAAACCAGAC TACGCCACTC ACGGTGGTAT CCTTTTCAAG 
TACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077542.1
CDS	126..2054
Translation	MVAPALLPELWTEILVPICAVIGIAFSLFQWYVVSRVKLTSDLGASSSGGANNGKNGYGDYLIEEEEGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEGFSTDNKPCTYDTTRTCKPALATAAFSTIAFVLGAVTSVLSGFLGMKIATYANARTTLEARKGVGKAFIVAFRSGAVMGFLLAASGLLVLYITINVFKIYYGDDWEGLFEAITGYGLGGSSMALFGRVGGGIYTKAADVGADLVGKIERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAEASCAALVVASISSFGINHDFTAMCYPLLISSMGILVCLITTLFATDFFEIKLVKEIEPALKNQLIISTVIMTVGIAIVSWVGLPTSFTIFNFGTQKVVKNWQLFLCVCVGLWAGLIIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAISIFVSFSFAAMYGVAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKGFAIGSAALVSLALFGAFVSRAGIHTVDVLTPKVIIGLLVGAMLPYWFSAMTMKSVGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATHGGILFKYF

Target ORF information:

RefSeq Version	NM_001084073.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Inorganic H pyrophosphatase family protein (AVP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001084073.1

ORF Insert Sequence:

ATGGTGGCGC CTGCTTTGTT ACCGGAGCTC TGGACGGAGA TCCTTGTACC GATTTGTGCG 
GTGATTGGTA TCGCCTTTTC GCTTTTCCAA TGGTACGTTG TATCTCGCGT GAAACTCACC 
TCTGACCTCG GCGCATCGTC TTCCGGTGGA GCTAACAATG GGAAGAATGG ATACGGTGAT 
TATCTAATCG AGGAAGAGGA AGGTGTTAAT GACCAGAGTG TTGTCGCTAA GTGCGCTGAG 
ATTCAGACTG CTATTTCCGA AGGTGCAACT TCATTCCTAT TCACGGAGTA CAAATATGTT 
GGTGTCTTCA TGATTTTCTT TGCTGCTGTT ATCTTTGTTT TCCTCGGCTC TGTTGAGGGA 
TTCAGCACTG ATAACAAGCC TTGTACTTAC GACACCACCA GAACCTGCAA GCCTGCATTG 
GCTACTGCAG CTTTCAGTAC CATTGCTTTC GTGCTTGGTG CTGTTACCTC TGTTCTATCT 
GGTTTCCTTG GGATGAAGAT TGCTACATAC GCTAATGCTA GGACCACTTT GGAGGCGAGG 
AAAGGTGTTG GAAAGGCGTT CATTGTTGCA TTCAGGTCTG GTGCTGTGAT GGGTTTCCTT 
CTTGCAGCGA GTGGTCTATT GGTGCTTTAC ATTACTATCA ATGTGTTCAA GATCTATTAC 
GGAGATGACT GGGAAGGTCT TTTTGAGGCT ATTACTGGTT ATGGTCTTGG TGGGTCTTCC 
ATGGCTCTCT TTGGCCGTGT TGGTGGTGGG ATCTACACTA AGGCTGCTGA TGTCGGCGCT 
GACCTTGTCG GTAAAATTGA GAGGAATATT CCAGAGGATG ATCCAAGAAA CCCAGCTGTC 
ATTGCTGATA ATGTCGGTGA CAATGTTGGT GACATTGCTG GTATGGGATC TGATCTCTTT 
GGATCATATG CTGAAGCATC ATGCGCTGCT CTTGTTGTTG CCTCGATCTC ATCTTTCGGA 
ATCAACCACG ACTTCACTGC CATGTGCTAC CCATTGCTCA TCAGTTCAAT GGGAATCTTG 
GTTTGTTTGA TCACAACTCT CTTTGCCACT GACTTCTTTG AGATTAAGCT TGTCAAGGAG 
ATTGAACCAG CATTGAAGAA CCAGCTCATT ATCTCAACTG TTATTATGAC TGTTGGTATT 
GCTATTGTGT CATGGGTTGG CTTACCGACC TCCTTTACCA TCTTCAACTT TGGAACACAA 
AAAGTTGTCA AGAACTGGCA GCTATTCCTT TGTGTTTGTG TTGGTCTTTG GGCTGGACTC 
ATTATTGGTT TCGTCACTGA GTACTACACT AGTAACGCCT ACAGCCCTGT GCAAGATGTT 
GCAGATTCAT GCAGAACTGG TGCAGCTACC AATGTTATCT TCGGCCTTGC TCTTGGTTAC 
AAATCCGTCA TTATTCCAAT CTTTGCTATT GCTATCAGTA TATTCGTTAG CTTCAGCTTT 
GCTGCTATGT ATGGTGTTGC TGTTGCTGCT CTTGGTATGC TCAGTACCAT TGCCACTGGT 
TTGGCAATTG ATGCTTATGG TCCCATCAGT GACAATGCTG GTGGTATTGC TGAAATGGCT 
GGAATGAGCC ACCGCATCCG TGAAAGAACT GATGCTCTTG ATGCCGCTGG AAACACCACT 
GCTGCTATTG GAAAGGGATT TGCCATTGGC TCTGCTGCCC TAGTCTCCTT GGCTCTCTTT 
GGTGCCTTTG TGAGCCGTGC AGGGATCCAC ACCGTAGATG TTTTGACCCC TAAAGTTATC 
ATTGGGCTCC TTGTTGGTGC CATGCTTCCT TACTGGTTCT CTGCCATGAC AATGAAGAGT 
GTGGGAAGTG CAGCTCTTAA GATGGTTGAA GAAGTTCGCA GGCAGTTCAA CACCATCCCT 
GGACTTATGG AAGGAACCGC AAAACCAGAC TACGCCACTC ACGGTGGTAT CCTTTTCAAG 
TACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AVP1 ( NM_101437.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_173021.1 Arabidopsis thaliana Inorganic H pyrophosphatase family protein (AVP1), mRNA.

Price & Availability↑

CloneID	OAb23661
Clone ID Related Accession (Same CDS sequence)	NM_101437.5
Accession Version	NM_101437.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Inorganic H pyrophosphatase family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_101437.4.

ATGGTGGCGC CTGCTTTGTT ACCGGAGCTC TGGACGGAGA TCCTTGTACC GATTTGTGCG 
GTGATTGGTA TCGCCTTTTC GCTTTTCCAA TGGTACGTTG TATCTCGCGT GAAACTCACC 
TCTGACCTCG GCGCATCGTC TTCCGGTGGA GCTAACAATG GGAAGAATGG ATACGGTGAT 
TATCTAATCG AGGAAGAGGA AGGTGTTAAT GACCAGAGTG TTGTCGCTAA GTGCGCTGAG 
ATTCAGACTG CTATTTCCGA AGGTGCAACT TCATTCCTAT TCACGGAGTA CAAATATGTT 
GGTGTCTTCA TGATTTTCTT TGCTGCTGTT ATCTTTGTTT TCCTCGGCTC TGTTGAGGGA 
TTCAGCACTG ATAACAAGCC TTGTACTTAC GACACCACCA GAACCTGCAA GCCTGCATTG 
GCTACTGCAG CTTTCAGTAC CATTGCTTTC GTGCTTGGTG CTGTTACCTC TGTTCTATCT 
GGTTTCCTTG GGATGAAGAT TGCTACATAC GCTAATGCTA GGACCACTTT GGAGGCGAGG 
AAAGGTGTTG GAAAGGCGTT CATTGTTGCA TTCAGGTCTG GTGCTGTGAT GGGTTTCCTT 
CTTGCAGCGA GTGGTCTATT GGTGCTTTAC ATTACTATCA ATGTGTTCAA GATCTATTAC 
GGAGATGACT GGGAAGGTCT TTTTGAGGCT ATTACTGGTT ATGGTCTTGG TGGGTCTTCC 
ATGGCTCTCT TTGGCCGTGT TGGTGGTGGG ATCTACACTA AGGCTGCTGA TGTCGGCGCT 
GACCTTGTCG GTAAAATTGA GAGGAATATT CCAGAGGATG ATCCAAGAAA CCCAGCTGTC 
ATTGCTGATA ATGTCGGTGA CAATGTTGGT GACATTGCTG GTATGGGATC TGATCTCTTT 
GGATCATATG CTGAAGCATC ATGCGCTGCT CTTGTTGTTG CCTCGATCTC ATCTTTCGGA 
ATCAACCACG ACTTCACTGC CATGTGCTAC CCATTGCTCA TCAGTTCAAT GGGAATCTTG 
GTTTGTTTGA TCACAACTCT CTTTGCCACT GACTTCTTTG AGATTAAGCT TGTCAAGGAG 
ATTGAACCAG CATTGAAGAA CCAGCTCATT ATCTCAACTG TTATTATGAC TGTTGGTATT 
GCTATTGTGT CATGGGTTGG CTTACCGACC TCCTTTACCA TCTTCAACTT TGGAACACAA 
AAAGTTGTCA AGAACTGGCA GCTATTCCTT TGTGTTTGTG TTGGTCTTTG GGCTGGACTC 
ATTATTGGTT TCGTCACTGA GTACTACACT AGTAACGCCT ACAGCCCTGT GCAAGATGTT 
GCAGATTCAT GCAGAACTGG TGCAGCTACC AATGTTATCT TCGGCCTTGC TCTTGGTTAC 
AAATCCGTCA TTATTCCAAT CTTTGCTATT GCTATCAGTA TATTCGTTAG CTTCAGCTTT 
GCTGCTATGT ATGGTGTTGC TGTTGCTGCT CTTGGTATGC TCAGTACCAT TGCCACTGGT 
TTGGCAATTG ATGCTTATGG TCCCATCAGT GACAATGCTG GTGGTATTGC TGAAATGGCT 
GGAATGAGCC ACCGCATCCG TGAAAGAACT GATGCTCTTG ATGCCGCTGG AAACACCACT 
GCTGCTATTG GAAAGGGATT TGCCATTGGC TCTGCTGCCC TAGTCTCCTT GGCTCTCTTT 
GGTGCCTTTG TGAGCCGTGC AGGGATCCAC ACCGTAGATG TTTTGACCCC TAAAGTTATC 
ATTGGGCTCC TTGTTGGTGC CATGCTTCCT TACTGGTTCT CTGCCATGAC AATGAAGAGT 
GTGGGAAGTG CAGCTCTTAA GATGGTTGAA GAAGTTCGCA GGCAGTTCAA CACCATCCCT 
GGACTTATGG AAGGAACCGC AAAACCAGAC TACGCCACAT GTGTCAAGAT CTCCACCGAT 
GCTTCCATCA AGGAAATGAT ACCTCCTGGT TGCCTTGTCA TGCTCACACC TCTCATTGTT 
GGTTTCTTCT TTGGAGTTGA GACCCTCTCT GGTGTCCTCG CCGGATCTCT TGTATCCGGT 
GTTCAGATCG CCATATCAGC ATCTAACACT GGTGGTGCCT GGGACAACGC CAAGAAATAC 
ATCGAGGCTG GTGTATCAGA GCACGCAAAG AGCCTTGGAC CAAAGGGTTC AGAGCCACAC 
AAGGCAGCTG TGATTGGAGA CACAATTGGA GACCCATTGA AGGATACTTC AGGACCTTCA 
TTGAACATCC TCATCAAGCT CATGGCTGTT GAGTCTCTTG TCTTTGCTCC CTTCTTCGCC 
ACTCACGGTG GTATCCTTTT CAAGTACTTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_173021.1
CDS	190..2502
Translation	MVAPALLPELWTEILVPICAVIGIAFSLFQWYVVSRVKLTSDLGASSSGGANNGKNGYGDYLIEEEEGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEGFSTDNKPCTYDTTRTCKPALATAAFSTIAFVLGAVTSVLSGFLGMKIATYANARTTLEARKGVGKAFIVAFRSGAVMGFLLAASGLLVLYITINVFKIYYGDDWEGLFEAITGYGLGGSSMALFGRVGGGIYTKAADVGADLVGKIERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAEASCAALVVASISSFGINHDFTAMCYPLLISSMGILVCLITTLFATDFFEIKLVKEIEPALKNQLIISTVIMTVGIAIVSWVGLPTSFTIFNFGTQKVVKNWQLFLCVCVGLWAGLIIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAISIFVSFSFAAMYGVAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKGFAIGSAALVSLALFGAFVSRAGIHTVDVLTPKVIIGLLVGAMLPYWFSAMTMKSVGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGCLVMLTPLIVGFFFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGVSEHAKSLGPKGSEPHKAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGILFKYF

Target ORF information:

RefSeq Version	NM_101437.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Inorganic H pyrophosphatase family protein (AVP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_101437.5

ORF Insert Sequence:

ATGGTGGCGC CTGCTTTGTT ACCGGAGCTC TGGACGGAGA TCCTTGTACC GATTTGTGCG 
GTGATTGGTA TCGCCTTTTC GCTTTTCCAA TGGTACGTTG TATCTCGCGT GAAACTCACC 
TCTGACCTCG GCGCATCGTC TTCCGGTGGA GCTAACAATG GGAAGAATGG ATACGGTGAT 
TATCTAATCG AGGAAGAGGA AGGTGTTAAT GACCAGAGTG TTGTCGCTAA GTGCGCTGAG 
ATTCAGACTG CTATTTCCGA AGGTGCAACT TCATTCCTAT TCACGGAGTA CAAATATGTT 
GGTGTCTTCA TGATTTTCTT TGCTGCTGTT ATCTTTGTTT TCCTCGGCTC TGTTGAGGGA 
TTCAGCACTG ATAACAAGCC TTGTACTTAC GACACCACCA GAACCTGCAA GCCTGCATTG 
GCTACTGCAG CTTTCAGTAC CATTGCTTTC GTGCTTGGTG CTGTTACCTC TGTTCTATCT 
GGTTTCCTTG GGATGAAGAT TGCTACATAC GCTAATGCTA GGACCACTTT GGAGGCGAGG 
AAAGGTGTTG GAAAGGCGTT CATTGTTGCA TTCAGGTCTG GTGCTGTGAT GGGTTTCCTT 
CTTGCAGCGA GTGGTCTATT GGTGCTTTAC ATTACTATCA ATGTGTTCAA GATCTATTAC 
GGAGATGACT GGGAAGGTCT TTTTGAGGCT ATTACTGGTT ATGGTCTTGG TGGGTCTTCC 
ATGGCTCTCT TTGGCCGTGT TGGTGGTGGG ATCTACACTA AGGCTGCTGA TGTCGGCGCT 
GACCTTGTCG GTAAAATTGA GAGGAATATT CCAGAGGATG ATCCAAGAAA CCCAGCTGTC 
ATTGCTGATA ATGTCGGTGA CAATGTTGGT GACATTGCTG GTATGGGATC TGATCTCTTT 
GGATCATATG CTGAAGCATC ATGCGCTGCT CTTGTTGTTG CCTCGATCTC ATCTTTCGGA 
ATCAACCACG ACTTCACTGC CATGTGCTAC CCATTGCTCA TCAGTTCAAT GGGAATCTTG 
GTTTGTTTGA TCACAACTCT CTTTGCCACT GACTTCTTTG AGATTAAGCT TGTCAAGGAG 
ATTGAACCAG CATTGAAGAA CCAGCTCATT ATCTCAACTG TTATTATGAC TGTTGGTATT 
GCTATTGTGT CATGGGTTGG CTTACCGACC TCCTTTACCA TCTTCAACTT TGGAACACAA 
AAAGTTGTCA AGAACTGGCA GCTATTCCTT TGTGTTTGTG TTGGTCTTTG GGCTGGACTC 
ATTATTGGTT TCGTCACTGA GTACTACACT AGTAACGCCT ACAGCCCTGT GCAAGATGTT 
GCAGATTCAT GCAGAACTGG TGCAGCTACC AATGTTATCT TCGGCCTTGC TCTTGGTTAC 
AAATCCGTCA TTATTCCAAT CTTTGCTATT GCTATCAGTA TATTCGTTAG CTTCAGCTTT 
GCTGCTATGT ATGGTGTTGC TGTTGCTGCT CTTGGTATGC TCAGTACCAT TGCCACTGGT 
TTGGCAATTG ATGCTTATGG TCCCATCAGT GACAATGCTG GTGGTATTGC TGAAATGGCT 
GGAATGAGCC ACCGCATCCG TGAAAGAACT GATGCTCTTG ATGCCGCTGG AAACACCACT 
GCTGCTATTG GAAAGGGATT TGCCATTGGC TCTGCTGCCC TAGTCTCCTT GGCTCTCTTT 
GGTGCCTTTG TGAGCCGTGC AGGGATCCAC ACCGTAGATG TTTTGACCCC TAAAGTTATC 
ATTGGGCTCC TTGTTGGTGC CATGCTTCCT TACTGGTTCT CTGCCATGAC AATGAAGAGT 
GTGGGAAGTG CAGCTCTTAA GATGGTTGAA GAAGTTCGCA GGCAGTTCAA CACCATCCCT 
GGACTTATGG AAGGAACCGC AAAACCAGAC TACGCCACAT GTGTCAAGAT CTCCACCGAT 
GCTTCCATCA AGGAAATGAT ACCTCCTGGT TGCCTTGTCA TGCTCACACC TCTCATTGTT 
GGTTTCTTCT TTGGAGTTGA GACCCTCTCT GGTGTCCTCG CCGGATCTCT TGTATCCGGT 
GTTCAGATCG CCATATCAGC ATCTAACACT GGTGGTGCCT GGGACAACGC CAAGAAATAC 
ATCGAGGCTG GTGTATCAGA GCACGCAAAG AGCCTTGGAC CAAAGGGTTC AGAGCCACAC 
AAGGCAGCTG TGATTGGAGA CACAATTGGA GACCCATTGA AGGATACTTC AGGACCTTCA 
TTGAACATCC TCATCAAGCT CATGGCTGTT GAGTCTCTTG TCTTTGCTCC CTTCTTCGCC 
ACTCACGGTG GTATCCTTTT CAAGTACTTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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AVP1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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