BON1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	BON1
Entrez Gene ID	836311
Full Name	Calcium-dependent phospholipid-binding Copine family protein
Synonyms	BON,BONZAI 1,COPINE 1,CPN1,K22G18.2,K22G18_2
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_203248.2	NP_974977.1	Calcium-dependent phospholipid-binding Copine family protein
NM_125583.4	NP_568944.1	Calcium-dependent phospholipid-binding Copine family protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	CPNE3	NP_003900.1
Rattus norvegicus (Norway rat)	Cpne3	NP_001101387.2
Arabidopsis thaliana (thale cress)	AT5G61910	NP_001190590.1
Arabidopsis thaliana (thale cress)	BON1	NP_568944.1
Pan troglodytes (chimpanzee)	CPNE3	XP_519845.3
Bos taurus (cattle)	CPNE3	NP_001092612.1
Arabidopsis thaliana (thale cress)	BON2	NP_568180.1
Oryza sativa (rice)	Os02g0521300	NP_001046976.1
Xenopus tropicalis (tropical clawed frog)	cpne3	NP_001008001.1
	CPNE3	XP_001082722.2
Mus musculus (house mouse)	Cpne3	NP_082045.1
Canis lupus familiaris (dog)	CPNE3	XP_544163.3
Gallus gallus (chicken)	CPNE3	XP_418324.2
Danio rerio (zebrafish)	cpne3	NP_956461.1
Arabidopsis thaliana (thale cress)	BON3	NP_172362.3

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

BON1 and BIR1 might modulate both PAMP- and R protein-triggered immune responses.
Title: BON1 interacts with the protein kinases BIR1 and BAK1 in modulation of temperature-dependent plant growth and cell death in Arabidopsis.

Requirement of calcium binding, myristoylation, and protein-protein interaction for the Copine BON1 function in Arabidopsis.
Title: Requirement of calcium binding, myristoylation, and protein-protein interaction for the Copine BON1 function in Arabidopsis.

BON1 and BON3 represses the transcript accumulation and/or protein activities of multiple resistance (R)-like genes.
Title: Multiple R-like genes are negatively regulated by BON1 and BON3 in arabidopsis.

Promoter deletion analysis identified a 280 bp segment of the BON1/CPN1 promoter as being required for pathogen-induced gene expression; the same promoter region was also required for calcium ionophore-induced gene expression
Title: Evidence that the BONZAI1/COPINE1 protein is a calcium- and pathogen-responsive defense suppressor.

Overexpression of BAP1 or BAP2 with their partner BON1 inhibits programmed cell death induced by pathogens, the proapoptotic gene BAX, and superoxide-generating paraquat.
Title: The Arabidopsis BAP1 and BAP2 genes are general inhibitors of programmed cell death.

The following BON1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BON1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb22149	NM_203248.2 Latest version!	Arabidopsis thaliana Calcium-dependent phospholipid-binding Copine family protein (BON1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OAb22149	NM_125583.4 Latest version!	Arabidopsis thaliana Calcium-dependent phospholipid-binding Copine family protein (BON1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BON1 ( NM_203248.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_974977.1 Arabidopsis thaliana Calcium-dependent phospholipid-binding Copine family protein (BON1), mRNA.

Price & Availability↑

CloneID	OAb22149
Clone ID Related Accession (Same CDS sequence)	NM_203248.2 , NM_125583.4
Accession Version	NM_203248.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Calcium-dependent phospholipid-binding Copine family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Apr 18, 2007 this sequence version replaced NM_203248.1.

ATGGGGAATT GTTGCTCCGA TGTCGCCTCC GGTGCTGGTG CTACAGCCGG CGTTGGTGGA 
AGTGGTTCCT CCGCCGCTCT CGGTGCGACT AACGACGCCC TTGATTACTA TCTCAAGTCC 
AAAGGTTTCA ACGGACTCTT CTCGCAGATA GAGCTATCGT TTTCAGCTTC GAATTTGCGA 
GACCGCGACG TGCTCTCCAA GAGTGATCCT ATGGTTGTTG TATATCAAAA AGAAAAAGAT 
GCAACACTTT CAGAAGTTTT CCGCAGTGAA GTTGTTTTGA ACTCTTTAGC TCCGAAATGG 
ATTAAGAAAT TTATAGTCGC TTATCATTTC GAGACTGTGC AGACATTGGT GTTTCGTGTG 
TATGATGTTG ACACCAAATT TCAAAATTCA AGAGAGGAGA TGCTTAAGCT TGACGAACAG 
CAGTTTCTTG GTGAGGCAAC ATGTGCATTG TCTGAGATAA TCACAAAATC AACTAGGACG 
AGCACATTAG AACTCAAGCG CAAAGATGGC TTTGCTCCAC AGGCTCAGCC TCACCACGGA 
AAGCTTATCA TCCATGCTGA GGAGTCCCTT GCTTCTAAGA TTTCAACAGA GATAGTATTT 
AGATGTTCGA ATTTGGAATC TAAGGATCTT TTTTCAAAAA GTGACCCCTT TTTGGTGGTA 
TCAAAGATTG TAGAGCATGG AACTCCAATT CCCGTGTCTA AAACTGAAGT CCGGAAGAAT 
GATCTTAACC CTATTTGGAA GCCAGTCTTT CTTAGTGTTC AACAAGTGGG AAGTAAGGAC 
AGTCCAGTGA TAATAGAATG CTCAGACTTT AACTCCAATG GCAAACACAG TCTGATTGGG 
AAAGTTCAGA AATCGCTTTC AGACTTAGAA AAACTTCATT TGGCGGGCCA AGGAATCAAT 
TTTTCTTTGC CTACTGGTGC TGGACAAAAC AAGGTGTTAA AGAGCCAACT TTTTGTGGAC 
AAGTTTACCG AGACTGTCCA CCACACATTC CTAGAGTACT TGGCATCTGG ATTTGAGTTA 
AACTTTATGG TAGCAATCGA TTTCACAGCT TCAAATGGAA ATCCCCGCCT CCCTGATTCT 
TTGCACTACA TTGATCCTTC TGGACGATTA AATGCCTACC AGAGAGCAAT AATGGATGTT 
GGAGAAGTGT TGCAGTTTTA TGACTCAGAC AAACGTTTCC CTGCCTGGGG ATTCGGAGCT 
CGTCCAATCG ACGCTCCAGT TTCACATTGC TTTAATCTAA ATGGAAGCAG TTCATACTCT 
GAGGTGGATG GGATTCAAGG AATCATGACT TCATACACCA GTGCCCTTTT CAACGTCTCT 
CTTGCAGGGC CTACCCTTTT TGGTCCAGTG ATCAACGCTG CTGCAATGAT CGCCAGCGCG 
TCGCTAGCTC AAGGTTCACG GAAATATTAC GTCTTACTGA TCATAACAGA TGGTGTTATA 
ACTGATCTTC AAGAAACAAA AGACGCATTA GTCAGTGCAT CGGATTTGCC CTTATCAATC 
CTCATTGTAG GAGTTGGAGG AGCTGATTTT AAAGAGATGG AGATACTAGA CGCAGATAAA 
GGAGAAAGAT TGGAGAGTTC GAGCGGGCGA TTGGCTTCAC GAGACATTGT TCAGTTTGTG 
GCACTACGAG ATGTCCAATA TGGAGAGATC TCAGTGGTAC AAGCGCTTCT AGCAGAATTG 
CCTTCGCAGT TTTTGACGTA TATGAGAATC CGTAATATGA AACCGATTCC TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_974977.1
CDS	123..1859
Translation	MGNCCSDVASGAGATAGVGGSGSSAALGATNDALDYYLKSKGFNGLFSQIELSFSASNLRDRDVLSKSDPMVVVYQKEKDATLSEVFRSEVVLNSLAPKWIKKFIVAYHFETVQTLVFRVYDVDTKFQNSREEMLKLDEQQFLGEATCALSEIITKSTRTSTLELKRKDGFAPQAQPHHGKLIIHAEESLASKISTEIVFRCSNLESKDLFSKSDPFLVVSKIVEHGTPIPVSKTEVRKNDLNPIWKPVFLSVQQVGSKDSPVIIECSDFNSNGKHSLIGKVQKSLSDLEKLHLAGQGINFSLPTGAGQNKVLKSQLFVDKFTETVHHTFLEYLASGFELNFMVAIDFTASNGNPRLPDSLHYIDPSGRLNAYQRAIMDVGEVLQFYDSDKRFPAWGFGARPIDAPVSHCFNLNGSSSYSEVDGIQGIMTSYTSALFNVSLAGPTLFGPVINAAAMIASASLAQGSRKYYVLLIITDGVITDLQETKDALVSASDLPLSILIVGVGGADFKEMEILDADKGERLESSSGRLASRDIVQFVALRDVQYGEISVVQALLAELPSQFLTYMRIRNMKPIPP

Target ORF information:

RefSeq Version	NM_203248.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Calcium-dependent phospholipid-binding Copine family protein (BON1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_203248.2

ORF Insert Sequence:

ATGGGGAATT GTTGCTCCGA TGTCGCCTCC GGTGCTGGTG CTACAGCCGG CGTTGGTGGA 
AGTGGTTCCT CCGCCGCTCT CGGTGCGACT AACGACGCCC TTGATTACTA TCTCAAGTCC 
AAAGGTTTCA ACGGACTCTT CTCGCAGATA GAGCTATCGT TTTCAGCTTC GAATTTGCGA 
GACCGCGACG TGCTCTCCAA GAGTGATCCT ATGGTTGTTG TATATCAAAA AGAAAAAGAT 
GCAACACTTT CAGAAGTTTT CCGCAGTGAA GTTGTTTTGA ACTCTTTAGC TCCGAAATGG 
ATTAAGAAAT TTATAGTCGC TTATCATTTC GAGACTGTGC AGACATTGGT GTTTCGTGTG 
TATGATGTTG ACACCAAATT TCAAAATTCA AGAGAGGAGA TGCTTAAGCT TGACGAACAG 
CAGTTTCTTG GTGAGGCAAC ATGTGCATTG TCTGAGATAA TCACAAAATC AACTAGGACG 
AGCACATTAG AACTCAAGCG CAAAGATGGC TTTGCTCCAC AGGCTCAGCC TCACCACGGA 
AAGCTTATCA TCCATGCTGA GGAGTCCCTT GCTTCTAAGA TTTCAACAGA GATAGTATTT 
AGATGTTCGA ATTTGGAATC TAAGGATCTT TTTTCAAAAA GTGACCCCTT TTTGGTGGTA 
TCAAAGATTG TAGAGCATGG AACTCCAATT CCCGTGTCTA AAACTGAAGT CCGGAAGAAT 
GATCTTAACC CTATTTGGAA GCCAGTCTTT CTTAGTGTTC AACAAGTGGG AAGTAAGGAC 
AGTCCAGTGA TAATAGAATG CTCAGACTTT AACTCCAATG GCAAACACAG TCTGATTGGG 
AAAGTTCAGA AATCGCTTTC AGACTTAGAA AAACTTCATT TGGCGGGCCA AGGAATCAAT 
TTTTCTTTGC CTACTGGTGC TGGACAAAAC AAGGTGTTAA AGAGCCAACT TTTTGTGGAC 
AAGTTTACCG AGACTGTCCA CCACACATTC CTAGAGTACT TGGCATCTGG ATTTGAGTTA 
AACTTTATGG TAGCAATCGA TTTCACAGCT TCAAATGGAA ATCCCCGCCT CCCTGATTCT 
TTGCACTACA TTGATCCTTC TGGACGATTA AATGCCTACC AGAGAGCAAT AATGGATGTT 
GGAGAAGTGT TGCAGTTTTA TGACTCAGAC AAACGTTTCC CTGCCTGGGG ATTCGGAGCT 
CGTCCAATCG ACGCTCCAGT TTCACATTGC TTTAATCTAA ATGGAAGCAG TTCATACTCT 
GAGGTGGATG GGATTCAAGG AATCATGACT TCATACACCA GTGCCCTTTT CAACGTCTCT 
CTTGCAGGGC CTACCCTTTT TGGTCCAGTG ATCAACGCTG CTGCAATGAT CGCCAGCGCG 
TCGCTAGCTC AAGGTTCACG GAAATATTAC GTCTTACTGA TCATAACAGA TGGTGTTATA 
ACTGATCTTC AAGAAACAAA AGACGCATTA GTCAGTGCAT CGGATTTGCC CTTATCAATC 
CTCATTGTAG GAGTTGGAGG AGCTGATTTT AAAGAGATGG AGATACTAGA CGCAGATAAA 
GGAGAAAGAT TGGAGAGTTC GAGCGGGCGA TTGGCTTCAC GAGACATTGT TCAGTTTGTG 
GCACTACGAG ATGTCCAATA TGGAGAGATC TCAGTGGTAC AAGCGCTTCT AGCAGAATTG 
CCTTCGCAGT TTTTGACGTA TATGAGAATC CGTAATATGA AACCGATTCC TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BON1 ( NM_125583.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_568944.1 Arabidopsis thaliana Calcium-dependent phospholipid-binding Copine family protein (BON1), mRNA.

Price & Availability↑

CloneID	OAb22149
Clone ID Related Accession (Same CDS sequence)	NM_203248.2 , NM_125583.4
Accession Version	NM_125583.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Calcium-dependent phospholipid-binding Copine family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Apr 24, 2008 this sequence version replaced NM_125583.3.

ATGGGGAATT GTTGCTCCGA TGTCGCCTCC GGTGCTGGTG CTACAGCCGG CGTTGGTGGA 
AGTGGTTCCT CCGCCGCTCT CGGTGCGACT AACGACGCCC TTGATTACTA TCTCAAGTCC 
AAAGGTTTCA ACGGACTCTT CTCGCAGATA GAGCTATCGT TTTCAGCTTC GAATTTGCGA 
GACCGCGACG TGCTCTCCAA GAGTGATCCT ATGGTTGTTG TATATCAAAA AGAAAAAGAT 
GCAACACTTT CAGAAGTTTT CCGCAGTGAA GTTGTTTTGA ACTCTTTAGC TCCGAAATGG 
ATTAAGAAAT TTATAGTCGC TTATCATTTC GAGACTGTGC AGACATTGGT GTTTCGTGTG 
TATGATGTTG ACACCAAATT TCAAAATTCA AGAGAGGAGA TGCTTAAGCT TGACGAACAG 
CAGTTTCTTG GTGAGGCAAC ATGTGCATTG TCTGAGATAA TCACAAAATC AACTAGGACG 
AGCACATTAG AACTCAAGCG CAAAGATGGC TTTGCTCCAC AGGCTCAGCC TCACCACGGA 
AAGCTTATCA TCCATGCTGA GGAGTCCCTT GCTTCTAAGA TTTCAACAGA GATAGTATTT 
AGATGTTCGA ATTTGGAATC TAAGGATCTT TTTTCAAAAA GTGACCCCTT TTTGGTGGTA 
TCAAAGATTG TAGAGCATGG AACTCCAATT CCCGTGTCTA AAACTGAAGT CCGGAAGAAT 
GATCTTAACC CTATTTGGAA GCCAGTCTTT CTTAGTGTTC AACAAGTGGG AAGTAAGGAC 
AGTCCAGTGA TAATAGAATG CTCAGACTTT AACTCCAATG GCAAACACAG TCTGATTGGG 
AAAGTTCAGA AATCGCTTTC AGACTTAGAA AAACTTCATT TGGCGGGCCA AGGAATCAAT 
TTTTCTTTGC CTACTGGTGC TGGACAAAAC AAGGTGTTAA AGAGCCAACT TTTTGTGGAC 
AAGTTTACCG AGACTGTCCA CCACACATTC CTAGAGTACT TGGCATCTGG ATTTGAGTTA 
AACTTTATGG TAGCAATCGA TTTCACAGCT TCAAATGGAA ATCCCCGCCT CCCTGATTCT 
TTGCACTACA TTGATCCTTC TGGACGATTA AATGCCTACC AGAGAGCAAT AATGGATGTT 
GGAGAAGTGT TGCAGTTTTA TGACTCAGAC AAACGTTTCC CTGCCTGGGG ATTCGGAGCT 
CGTCCAATCG ACGCTCCAGT TTCACATTGC TTTAATCTAA ATGGAAGCAG TTCATACTCT 
GAGGTGGATG GGATTCAAGG AATCATGACT TCATACACCA GTGCCCTTTT CAACGTCTCT 
CTTGCAGGGC CTACCCTTTT TGGTCCAGTG ATCAACGCTG CTGCAATGAT CGCCAGCGCG 
TCGCTAGCTC AAGGTTCACG GAAATATTAC GTCTTACTGA TCATAACAGA TGGTGTTATA 
ACTGATCTTC AAGAAACAAA AGACGCATTA GTCAGTGCAT CGGATTTGCC CTTATCAATC 
CTCATTGTAG GAGTTGGAGG AGCTGATTTT AAAGAGATGG AGATACTAGA CGCAGATAAA 
GGAGAAAGAT TGGAGAGTTC GAGCGGGCGA TTGGCTTCAC GAGACATTGT TCAGTTTGTG 
GCACTACGAG ATGTCCAATA TGGAGAGATC TCAGTGGTAC AAGCGCTTCT AGCAGAATTG 
CCTTCGCAGT TTTTGACGTA TATGAGAATC CGTAATATGA AACCGATTCC TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_568944.1
CDS	88..1824
Translation	MGNCCSDVASGAGATAGVGGSGSSAALGATNDALDYYLKSKGFNGLFSQIELSFSASNLRDRDVLSKSDPMVVVYQKEKDATLSEVFRSEVVLNSLAPKWIKKFIVAYHFETVQTLVFRVYDVDTKFQNSREEMLKLDEQQFLGEATCALSEIITKSTRTSTLELKRKDGFAPQAQPHHGKLIIHAEESLASKISTEIVFRCSNLESKDLFSKSDPFLVVSKIVEHGTPIPVSKTEVRKNDLNPIWKPVFLSVQQVGSKDSPVIIECSDFNSNGKHSLIGKVQKSLSDLEKLHLAGQGINFSLPTGAGQNKVLKSQLFVDKFTETVHHTFLEYLASGFELNFMVAIDFTASNGNPRLPDSLHYIDPSGRLNAYQRAIMDVGEVLQFYDSDKRFPAWGFGARPIDAPVSHCFNLNGSSSYSEVDGIQGIMTSYTSALFNVSLAGPTLFGPVINAAAMIASASLAQGSRKYYVLLIITDGVITDLQETKDALVSASDLPLSILIVGVGGADFKEMEILDADKGERLESSSGRLASRDIVQFVALRDVQYGEISVVQALLAELPSQFLTYMRIRNMKPIPP

Target ORF information:

RefSeq Version	NM_125583.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Calcium-dependent phospholipid-binding Copine family protein (BON1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_125583.4

ORF Insert Sequence:

ATGGGGAATT GTTGCTCCGA TGTCGCCTCC GGTGCTGGTG CTACAGCCGG CGTTGGTGGA 
AGTGGTTCCT CCGCCGCTCT CGGTGCGACT AACGACGCCC TTGATTACTA TCTCAAGTCC 
AAAGGTTTCA ACGGACTCTT CTCGCAGATA GAGCTATCGT TTTCAGCTTC GAATTTGCGA 
GACCGCGACG TGCTCTCCAA GAGTGATCCT ATGGTTGTTG TATATCAAAA AGAAAAAGAT 
GCAACACTTT CAGAAGTTTT CCGCAGTGAA GTTGTTTTGA ACTCTTTAGC TCCGAAATGG 
ATTAAGAAAT TTATAGTCGC TTATCATTTC GAGACTGTGC AGACATTGGT GTTTCGTGTG 
TATGATGTTG ACACCAAATT TCAAAATTCA AGAGAGGAGA TGCTTAAGCT TGACGAACAG 
CAGTTTCTTG GTGAGGCAAC ATGTGCATTG TCTGAGATAA TCACAAAATC AACTAGGACG 
AGCACATTAG AACTCAAGCG CAAAGATGGC TTTGCTCCAC AGGCTCAGCC TCACCACGGA 
AAGCTTATCA TCCATGCTGA GGAGTCCCTT GCTTCTAAGA TTTCAACAGA GATAGTATTT 
AGATGTTCGA ATTTGGAATC TAAGGATCTT TTTTCAAAAA GTGACCCCTT TTTGGTGGTA 
TCAAAGATTG TAGAGCATGG AACTCCAATT CCCGTGTCTA AAACTGAAGT CCGGAAGAAT 
GATCTTAACC CTATTTGGAA GCCAGTCTTT CTTAGTGTTC AACAAGTGGG AAGTAAGGAC 
AGTCCAGTGA TAATAGAATG CTCAGACTTT AACTCCAATG GCAAACACAG TCTGATTGGG 
AAAGTTCAGA AATCGCTTTC AGACTTAGAA AAACTTCATT TGGCGGGCCA AGGAATCAAT 
TTTTCTTTGC CTACTGGTGC TGGACAAAAC AAGGTGTTAA AGAGCCAACT TTTTGTGGAC 
AAGTTTACCG AGACTGTCCA CCACACATTC CTAGAGTACT TGGCATCTGG ATTTGAGTTA 
AACTTTATGG TAGCAATCGA TTTCACAGCT TCAAATGGAA ATCCCCGCCT CCCTGATTCT 
TTGCACTACA TTGATCCTTC TGGACGATTA AATGCCTACC AGAGAGCAAT AATGGATGTT 
GGAGAAGTGT TGCAGTTTTA TGACTCAGAC AAACGTTTCC CTGCCTGGGG ATTCGGAGCT 
CGTCCAATCG ACGCTCCAGT TTCACATTGC TTTAATCTAA ATGGAAGCAG TTCATACTCT 
GAGGTGGATG GGATTCAAGG AATCATGACT TCATACACCA GTGCCCTTTT CAACGTCTCT 
CTTGCAGGGC CTACCCTTTT TGGTCCAGTG ATCAACGCTG CTGCAATGAT CGCCAGCGCG 
TCGCTAGCTC AAGGTTCACG GAAATATTAC GTCTTACTGA TCATAACAGA TGGTGTTATA 
ACTGATCTTC AAGAAACAAA AGACGCATTA GTCAGTGCAT CGGATTTGCC CTTATCAATC 
CTCATTGTAG GAGTTGGAGG AGCTGATTTT AAAGAGATGG AGATACTAGA CGCAGATAAA 
GGAGAAAGAT TGGAGAGTTC GAGCGGGCGA TTGGCTTCAC GAGACATTGT TCAGTTTGTG 
GCACTACGAG ATGTCCAATA TGGAGAGATC TCAGTGGTAC AAGCGCTTCT AGCAGAATTG 
CCTTCGCAGT TTTTGACGTA TATGAGAATC CGTAATATGA AACCGATTCC TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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BON1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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