EMF2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	EMF2
Entrez Gene ID	835198
Full Name	VEFS-Box of polycomb protein
Synonyms	AT5G51240,AtEMF2,CYR1,CYTOKININ RESISTANT 1,EMBRYONIC FLOWER 2,MWD22.18,MWD22_18,VEF2
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001125941.1	NP_001119413.1	VEFS-Box of polycomb protein
NM_124502.3	NP_199936.2	VEFS-Box of polycomb protein
NM_180837.3	NP_851168.1	VEFS-Box of polycomb protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Knock-out mutants in identified candidate genes (CYR1, At1g32360 and RLP48) were isolated and differences in root hair traits in the mutants were confirmed.
Title: Uncovering genes and ploidy involved in the high diversity in root hair density, length and response to local scarce phosphate in Arabidopsis thaliana.

The observations demonstrate a functional cooperative interplay between SDG8 and an EMF2-containing PcG complex in maintaining vegetative cell identity by repressing seed genes to promote seedling development.
Title: Synergistic repression of the embryonic programme by SET DOMAIN GROUP 8 and EMBRYONIC FLOWER 2 in Arabidopsis seedlings.

to promote vegetative development and to regulate cell differentiation during shoot and root organogenesis, EMF1 binds genes required for other developmental phases. These are primarily genes trimethylated by EMF2-PRC2 on their H3K27
Title: EMF1 and PRC2 cooperate to repress key regulators of Arabidopsis development.

EMF1 and EMF2 target transcription factor genes to mediate vegetative development and stress responses, and regulate flowering time genes.
Title: Epigenetic regulation of gene programs by EMF1 and EMF2 in Arabidopsis.

The following EMF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EMF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb21171	NM_180837.3 Latest version!	Arabidopsis thaliana VEFS-Box of polycomb protein (EMF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OAb21172	NM_001125941.1 Latest version!	Arabidopsis thaliana VEFS-Box of polycomb protein (EMF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OAb21172	NM_124502.3 Latest version!	Arabidopsis thaliana VEFS-Box of polycomb protein (EMF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EMF2 ( NM_180837.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_851168.1 Arabidopsis thaliana VEFS-Box of polycomb protein (EMF2), mRNA.

Price & Availability↑

CloneID	OAb21171
Clone ID Related Accession (Same CDS sequence)	NM_180837.3
Accession Version	NM_180837.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	VEFS-Box of polycomb protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_180837.2.

ATGCCAGGCA TTCCTCTTGT TAGTCGTGAA ACCTCTTCTT GTTCAAGAAG CACAGAGCAG 
ATGTGCCATG AAGACTCCCG TCTGCGTATT TCGGAAGAGG AGGAGATTGC TGCTGAAGAG 
AGCTTGGCTG CCTATTGCAA GCCTGTTGAA CTCTACAATA TCATTCAACG CCGTGCTATT 
AGGAATCCCT TGTTTCTTCA GCGATGTTTG CATTATAAGA TTGAGGCAAA ACATAAAAGG 
AGAATACAAA TGACTGTATT CCTCTCGGGC GCTATAGATG CTGGGGTACA AACTCAAAAA 
TTATTCCCTC TGTATATTTT GTTGGCAAGA CTCGTTTCTC CTAAGCCTGT CGCTGAGTAT 
TCTGCAGTAT ATAGGTTCAG TCGAGCATGT ATCCTAACTG GTGGATTGGG GGTTGATGGA 
GTTAGTCAAG CCCAAGCCAA CTTTCTTCTC CCTGATATGA ATAGACTCGC ATTGGAGGCA 
AAATCAGGAT CACTCGCTAT CTTGTTTATC AGCTTTGCTG GTGCGCAAAA TTCTCAATTT 
GGCATTGATT CAGGCAAGAT TCATTCAGGA AATATAGGAG GACATTGTTT ATGGAGCAAA 
ATACCTCTGC AATCACTGTA TGCGTCGTGG CAGAAATCAC CAAACATGGA CTTGGGACAG 
AGAGTAGACA CAGTCTCTCT TGTTGAAATG CAGCCTTGCT TCATAAAGCT AAAGTCCATG 
AGTGAGGAAA AGTGTGTCTC GATTCAGGTG CCCAGCAATC CACTCACCTC GAGCTCTCCG 
CAGCAAGTGC AAGTCACCAT ATCTGCAGAA GAAGTTGGGT CAACGGAAAA ATCTCCTTAT 
AGTTCATTTT CATATAATGA CATCTCTTCC TCTTCCTTGT TGCAAATTAT CAGGTTGAGA 
ACAGGAAATG TAGTTTTCAA CTACAGATAC TATAACAACA AATTGCAGAA GACTGAAGTA 
ACTGAAGACT TTTCTTGTCC ATTCTGCTTA GTAAAATGTG CCAGTTTCAA GGGCCTGAGA 
TATCACTTGC CATCAACCCA CGATCTCCTC AATTTCGAGT TTTGGGTAAC TGAAGAATTT 
CAGGCGGTAA ATGTCTCCCT CAAGACTGAG ACAATGATAT CCAAGGTTAA TGAGGATGAC 
GTTGACCCAA AGCAGCAAAC TTTCTTTTTT TCTTCCAAAA AATTCAGACG GAGGAGGCAA 
AAGAGTCAGG TACGGAGCTC AAGGCAAGGG CCTCATCTTG GATTAGGTTG CGAGGTGCTA 
GATAAGACTG ATGATGCTCA TTCTGTTAGA AGTGAGAAGA GCCGAATACC ACCTGGAAAG 
CATTACGAAA GAATTGGGGG TGCTGAGTCT GGTCAAAGAG TTCCTCCTGG CACGAGTCCT 
GCAGACGTGC AATCATGTGG GGATCCAGAT TATGTGCAGT CGATAGCTGG AAGTACAATG 
TTGCAGTTTG CAAAAACGAG GAAAATATCT ATAGAACGGT CGGACTTGAG GAACCGAAGC 
CTCCTTCAGA AGAGACAGTT CTTCCACTCT CATCGAGCTC AGCCCATGGC TCTAGAACAA 
GTACTTTCGG ACCGGGATAG TGAAGATGAA GTTGATGATG ATGTGGCAGA TTTTGAAGAT 
AGAAGGATGC TCGATGATTT CGTTGATGTG ACTAAAGATG AGAAACAGAT GATGCACATG 
TGGAACTCGT TTGTGAGGAA GCAGCGAGTA TTAGCAGATG GTCACATTCC ATGGGCATGC 
GAGGCATTCT CAAGATTGCA CGGACCCATC ATGGTTCGAA CACCGCACTT GATTTGGTGC 
TGGAGAGTGT TTATGGTGAA ACTGTGGAAC CACGGTCTTC TTGATGCCCG AACCATGAAC 
AACTGTAATA CCTTTCTCGA ACAGCTCCAA ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_851168.1
CDS	404..2299
Translation	MPGIPLVSRETSSCSRSTEQMCHEDSRLRISEEEEIAAEESLAAYCKPVELYNIIQRRAIRNPLFLQRCLHYKIEAKHKRRIQMTVFLSGAIDAGVQTQKLFPLYILLARLVSPKPVAEYSAVYRFSRACILTGGLGVDGVSQAQANFLLPDMNRLALEAKSGSLAILFISFAGAQNSQFGIDSGKIHSGNIGGHCLWSKIPLQSLYASWQKSPNMDLGQRVDTVSLVEMQPCFIKLKSMSEEKCVSIQVPSNPLTSSSPQQVQVTISAEEVGSTEKSPYSSFSYNDISSSSLLQIIRLRTGNVVFNYRYYNNKLQKTEVTEDFSCPFCLVKCASFKGLRYHLPSTHDLLNFEFWVTEEFQAVNVSLKTETMISKVNEDDVDPKQQTFFFSSKKFRRRRQKSQVRSSRQGPHLGLGCEVLDKTDDAHSVRSEKSRIPPGKHYERIGGAESGQRVPPGTSPADVQSCGDPDYVQSIAGSTMLQFAKTRKISIERSDLRNRSLLQKRQFFHSHRAQPMALEQVLSDRDSEDEVDDDVADFEDRRMLDDFVDVTKDEKQMMHMWNSFVRKQRVLADGHIPWACEAFSRLHGPIMVRTPHLIWCWRVFMVKLWNHGLLDARTMNNCNTFLEQLQI

Target ORF information:

RefSeq Version	NM_180837.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana VEFS-Box of polycomb protein (EMF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_180837.3

ORF Insert Sequence:

ATGCCAGGCA TTCCTCTTGT TAGTCGTGAA ACCTCTTCTT GTTCAAGAAG CACAGAGCAG 
ATGTGCCATG AAGACTCCCG TCTGCGTATT TCGGAAGAGG AGGAGATTGC TGCTGAAGAG 
AGCTTGGCTG CCTATTGCAA GCCTGTTGAA CTCTACAATA TCATTCAACG CCGTGCTATT 
AGGAATCCCT TGTTTCTTCA GCGATGTTTG CATTATAAGA TTGAGGCAAA ACATAAAAGG 
AGAATACAAA TGACTGTATT CCTCTCGGGC GCTATAGATG CTGGGGTACA AACTCAAAAA 
TTATTCCCTC TGTATATTTT GTTGGCAAGA CTCGTTTCTC CTAAGCCTGT CGCTGAGTAT 
TCTGCAGTAT ATAGGTTCAG TCGAGCATGT ATCCTAACTG GTGGATTGGG GGTTGATGGA 
GTTAGTCAAG CCCAAGCCAA CTTTCTTCTC CCTGATATGA ATAGACTCGC ATTGGAGGCA 
AAATCAGGAT CACTCGCTAT CTTGTTTATC AGCTTTGCTG GTGCGCAAAA TTCTCAATTT 
GGCATTGATT CAGGCAAGAT TCATTCAGGA AATATAGGAG GACATTGTTT ATGGAGCAAA 
ATACCTCTGC AATCACTGTA TGCGTCGTGG CAGAAATCAC CAAACATGGA CTTGGGACAG 
AGAGTAGACA CAGTCTCTCT TGTTGAAATG CAGCCTTGCT TCATAAAGCT AAAGTCCATG 
AGTGAGGAAA AGTGTGTCTC GATTCAGGTG CCCAGCAATC CACTCACCTC GAGCTCTCCG 
CAGCAAGTGC AAGTCACCAT ATCTGCAGAA GAAGTTGGGT CAACGGAAAA ATCTCCTTAT 
AGTTCATTTT CATATAATGA CATCTCTTCC TCTTCCTTGT TGCAAATTAT CAGGTTGAGA 
ACAGGAAATG TAGTTTTCAA CTACAGATAC TATAACAACA AATTGCAGAA GACTGAAGTA 
ACTGAAGACT TTTCTTGTCC ATTCTGCTTA GTAAAATGTG CCAGTTTCAA GGGCCTGAGA 
TATCACTTGC CATCAACCCA CGATCTCCTC AATTTCGAGT TTTGGGTAAC TGAAGAATTT 
CAGGCGGTAA ATGTCTCCCT CAAGACTGAG ACAATGATAT CCAAGGTTAA TGAGGATGAC 
GTTGACCCAA AGCAGCAAAC TTTCTTTTTT TCTTCCAAAA AATTCAGACG GAGGAGGCAA 
AAGAGTCAGG TACGGAGCTC AAGGCAAGGG CCTCATCTTG GATTAGGTTG CGAGGTGCTA 
GATAAGACTG ATGATGCTCA TTCTGTTAGA AGTGAGAAGA GCCGAATACC ACCTGGAAAG 
CATTACGAAA GAATTGGGGG TGCTGAGTCT GGTCAAAGAG TTCCTCCTGG CACGAGTCCT 
GCAGACGTGC AATCATGTGG GGATCCAGAT TATGTGCAGT CGATAGCTGG AAGTACAATG 
TTGCAGTTTG CAAAAACGAG GAAAATATCT ATAGAACGGT CGGACTTGAG GAACCGAAGC 
CTCCTTCAGA AGAGACAGTT CTTCCACTCT CATCGAGCTC AGCCCATGGC TCTAGAACAA 
GTACTTTCGG ACCGGGATAG TGAAGATGAA GTTGATGATG ATGTGGCAGA TTTTGAAGAT 
AGAAGGATGC TCGATGATTT CGTTGATGTG ACTAAAGATG AGAAACAGAT GATGCACATG 
TGGAACTCGT TTGTGAGGAA GCAGCGAGTA TTAGCAGATG GTCACATTCC ATGGGCATGC 
GAGGCATTCT CAAGATTGCA CGGACCCATC ATGGTTCGAA CACCGCACTT GATTTGGTGC 
TGGAGAGTGT TTATGGTGAA ACTGTGGAAC CACGGTCTTC TTGATGCCCG AACCATGAAC 
AACTGTAATA CCTTTCTCGA ACAGCTCCAA ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMF2 ( NM_001125941.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001119413.1 Arabidopsis thaliana VEFS-Box of polycomb protein (EMF2), mRNA.

Price & Availability↑

CloneID	OAb21172
Clone ID Related Accession (Same CDS sequence)	NM_001125941.1 , NM_124502.3
Accession Version	NM_001125941.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	VEFS-Box of polycomb protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGCCAGGCA TTCCTCTTGT TAGTCGTGAA ACCTCTTCTT GTTCAAGAAG CACAGAGCAG 
ATGTGCCATG AAGACTCCCG TCTGCGTATT TCGGAAGAGG AGGAGATTGC TGCTGAAGAG 
AGCTTGGCTG CCTATTGCAA GCCTGTTGAA CTCTACAATA TCATTCAACG CCGTGCTATT 
AGGAATCCCT TGTTTCTTCA GCGATGTTTG CATTATAAGA TTGAGGCAAA ACATAAAAGG 
AGAATACAAA TGACTGTATT CCTCTCGGGC GCTATAGATG CTGGGGTACA AACTCAAAAA 
TTATTCCCTC TGTATATTTT GTTGGCAAGA CTCGTTTCTC CTAAGCCTGT CGCTGAGTAT 
TCTGCAGTAT ATAGGTTCAG TCGAGCATGT ATCCTAACTG GTGGATTGGG GGTTGATGGA 
GTTAGTCAAG CCCAAGCCAA CTTTCTTCTC CCTGATATGA ATAGACTCGC ATTGGAGGCA 
AAATCAGGAT CACTCGCTAT CTTGTTTATC AGCTTTGCTG GTGCGCAAAA TTCTCAATTT 
GGCATTGATT CAGGCAAGAT TCATTCAGGA AATATAGGAG GACATTGTTT ATGGAGCAAA 
ATACCTCTGC AATCACTGTA TGCGTCGTGG CAGAAATCAC CAAACATGGA CTTGGGACAG 
AGAGTAGACA CAGTCTCTCT TGTTGAAATG CAGCCTTGCT TCATAAAGCT AAAGTCCATG 
AGTGAGGAAA AGTGTGTCTC GATTCAGGTG CCCAGCAATC CACTCACCTC GAGCTCTCCG 
CAGCAAGTGC AAGTCACCAT ATCTGCAGAA GAAGTTGGGT CAACGGAAAA ATCTCCTTAT 
AGTTCATTTT CATATAATGA CATCTCTTCC TCTTCCTTGT TGCAAATTAT CAGGTTGAGA 
ACAGGAAATG TAGTTTTCAA CTACAGATAC TATAACAACA AATTGCAGAA GACTGAAGTA 
ACTGAAGACT TTTCTTGTCC ATTCTGCTTA GTAAAATGTG CCAGTTTCAA GGGCCTGAGA 
TATCACTTGC CATCAACCCA CGATCTCCTC AATTTCGAGT TTTGGGTAAC TGAAGAATTT 
CAGGCGGTAA ATGTCTCCCT CAAGACTGAG ACAATGATAT CCAAGGTTAA TGAGGATGAC 
GTTGACCCAA AGCAGCAAAC TTTCTTTTTT TCACGGAGGA GGCAAAAGAG TCAGGTACGG 
AGCTCAAGGC AAGGGCCTCA TCTTGGATTA GGTTGCGAGG TGCTAGATAA GACTGATGAT 
GCTCATTCTG TTAGAAGTGA GAAGAGCCGA ATACCACCTG GAAAGCATTA CGAAAGAATT 
GGGGGTGCTG AGTCTGGTCA AAGAGTTCCT CCTGGCACGA GTCCTGCAGA CGTGCAATCA 
TGTGGGGATC CAGATTATGT GCAGTCGATA GCTGGAAGTA CAATGTTGCA GTTTGCAAAA 
ACGAGGAAAA TATCTATAGA ACGGTCGGAC TTGAGGAACC GAAGCCTCCT TCAGAAGAGA 
CAGTTCTTCC ACTCTCATCG AGCTCAGCCC ATGGCTCTAG AACAAGTACT TTCGGACCGG 
GATAGTGAAG ATGAAGTTGA TGATGATGTG GCAGATTTTG AAGATAGAAG GATGCTCGAT 
GATTTCGTTG ATGTGACTAA AGATGAGAAA CAGATGATGC ACATGTGGAA CTCGTTTGTG 
AGGAAGCAGC GAGTATTAGC AGATGGTCAC ATTCCATGGG CATGCGAGGC ATTCTCAAGA 
TTGCACGGAC CCATCATGGT TCGAACACCG CACTTGATTT GGTGCTGGAG AGTGTTTATG 
GTGAAACTGT GGAACCACGG TCTTCTTGAT GCCCGAACCA TGAACAACTG TAATACCTTT 
CTCGAACAGC TCCAAATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001119413.1
CDS	320..2200
Translation	MPGIPLVSRETSSCSRSTEQMCHEDSRLRISEEEEIAAEESLAAYCKPVELYNIIQRRAIRNPLFLQRCLHYKIEAKHKRRIQMTVFLSGAIDAGVQTQKLFPLYILLARLVSPKPVAEYSAVYRFSRACILTGGLGVDGVSQAQANFLLPDMNRLALEAKSGSLAILFISFAGAQNSQFGIDSGKIHSGNIGGHCLWSKIPLQSLYASWQKSPNMDLGQRVDTVSLVEMQPCFIKLKSMSEEKCVSIQVPSNPLTSSSPQQVQVTISAEEVGSTEKSPYSSFSYNDISSSSLLQIIRLRTGNVVFNYRYYNNKLQKTEVTEDFSCPFCLVKCASFKGLRYHLPSTHDLLNFEFWVTEEFQAVNVSLKTETMISKVNEDDVDPKQQTFFFSRRRQKSQVRSSRQGPHLGLGCEVLDKTDDAHSVRSEKSRIPPGKHYERIGGAESGQRVPPGTSPADVQSCGDPDYVQSIAGSTMLQFAKTRKISIERSDLRNRSLLQKRQFFHSHRAQPMALEQVLSDRDSEDEVDDDVADFEDRRMLDDFVDVTKDEKQMMHMWNSFVRKQRVLADGHIPWACEAFSRLHGPIMVRTPHLIWCWRVFMVKLWNHGLLDARTMNNCNTFLEQLQI

Target ORF information:

RefSeq Version	NM_001125941.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana VEFS-Box of polycomb protein (EMF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001125941.1

ORF Insert Sequence:

ATGCCAGGCA TTCCTCTTGT TAGTCGTGAA ACCTCTTCTT GTTCAAGAAG CACAGAGCAG 
ATGTGCCATG AAGACTCCCG TCTGCGTATT TCGGAAGAGG AGGAGATTGC TGCTGAAGAG 
AGCTTGGCTG CCTATTGCAA GCCTGTTGAA CTCTACAATA TCATTCAACG CCGTGCTATT 
AGGAATCCCT TGTTTCTTCA GCGATGTTTG CATTATAAGA TTGAGGCAAA ACATAAAAGG 
AGAATACAAA TGACTGTATT CCTCTCGGGC GCTATAGATG CTGGGGTACA AACTCAAAAA 
TTATTCCCTC TGTATATTTT GTTGGCAAGA CTCGTTTCTC CTAAGCCTGT CGCTGAGTAT 
TCTGCAGTAT ATAGGTTCAG TCGAGCATGT ATCCTAACTG GTGGATTGGG GGTTGATGGA 
GTTAGTCAAG CCCAAGCCAA CTTTCTTCTC CCTGATATGA ATAGACTCGC ATTGGAGGCA 
AAATCAGGAT CACTCGCTAT CTTGTTTATC AGCTTTGCTG GTGCGCAAAA TTCTCAATTT 
GGCATTGATT CAGGCAAGAT TCATTCAGGA AATATAGGAG GACATTGTTT ATGGAGCAAA 
ATACCTCTGC AATCACTGTA TGCGTCGTGG CAGAAATCAC CAAACATGGA CTTGGGACAG 
AGAGTAGACA CAGTCTCTCT TGTTGAAATG CAGCCTTGCT TCATAAAGCT AAAGTCCATG 
AGTGAGGAAA AGTGTGTCTC GATTCAGGTG CCCAGCAATC CACTCACCTC GAGCTCTCCG 
CAGCAAGTGC AAGTCACCAT ATCTGCAGAA GAAGTTGGGT CAACGGAAAA ATCTCCTTAT 
AGTTCATTTT CATATAATGA CATCTCTTCC TCTTCCTTGT TGCAAATTAT CAGGTTGAGA 
ACAGGAAATG TAGTTTTCAA CTACAGATAC TATAACAACA AATTGCAGAA GACTGAAGTA 
ACTGAAGACT TTTCTTGTCC ATTCTGCTTA GTAAAATGTG CCAGTTTCAA GGGCCTGAGA 
TATCACTTGC CATCAACCCA CGATCTCCTC AATTTCGAGT TTTGGGTAAC TGAAGAATTT 
CAGGCGGTAA ATGTCTCCCT CAAGACTGAG ACAATGATAT CCAAGGTTAA TGAGGATGAC 
GTTGACCCAA AGCAGCAAAC TTTCTTTTTT TCACGGAGGA GGCAAAAGAG TCAGGTACGG 
AGCTCAAGGC AAGGGCCTCA TCTTGGATTA GGTTGCGAGG TGCTAGATAA GACTGATGAT 
GCTCATTCTG TTAGAAGTGA GAAGAGCCGA ATACCACCTG GAAAGCATTA CGAAAGAATT 
GGGGGTGCTG AGTCTGGTCA AAGAGTTCCT CCTGGCACGA GTCCTGCAGA CGTGCAATCA 
TGTGGGGATC CAGATTATGT GCAGTCGATA GCTGGAAGTA CAATGTTGCA GTTTGCAAAA 
ACGAGGAAAA TATCTATAGA ACGGTCGGAC TTGAGGAACC GAAGCCTCCT TCAGAAGAGA 
CAGTTCTTCC ACTCTCATCG AGCTCAGCCC ATGGCTCTAG AACAAGTACT TTCGGACCGG 
GATAGTGAAG ATGAAGTTGA TGATGATGTG GCAGATTTTG AAGATAGAAG GATGCTCGAT 
GATTTCGTTG ATGTGACTAA AGATGAGAAA CAGATGATGC ACATGTGGAA CTCGTTTGTG 
AGGAAGCAGC GAGTATTAGC AGATGGTCAC ATTCCATGGG CATGCGAGGC ATTCTCAAGA 
TTGCACGGAC CCATCATGGT TCGAACACCG CACTTGATTT GGTGCTGGAG AGTGTTTATG 
GTGAAACTGT GGAACCACGG TCTTCTTGAT GCCCGAACCA TGAACAACTG TAATACCTTT 
CTCGAACAGC TCCAAATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMF2 ( NM_124502.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_199936.2 Arabidopsis thaliana VEFS-Box of polycomb protein (EMF2), mRNA.

Price & Availability↑

CloneID	OAb21172
Clone ID Related Accession (Same CDS sequence)	NM_001125941.1 , NM_124502.3
Accession Version	NM_124502.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	VEFS-Box of polycomb protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Apr 18, 2007 this sequence version replaced NM_124502.2.

ATGCCAGGCA TTCCTCTTGT TAGTCGTGAA ACCTCTTCTT GTTCAAGAAG CACAGAGCAG 
ATGTGCCATG AAGACTCCCG TCTGCGTATT TCGGAAGAGG AGGAGATTGC TGCTGAAGAG 
AGCTTGGCTG CCTATTGCAA GCCTGTTGAA CTCTACAATA TCATTCAACG CCGTGCTATT 
AGGAATCCCT TGTTTCTTCA GCGATGTTTG CATTATAAGA TTGAGGCAAA ACATAAAAGG 
AGAATACAAA TGACTGTATT CCTCTCGGGC GCTATAGATG CTGGGGTACA AACTCAAAAA 
TTATTCCCTC TGTATATTTT GTTGGCAAGA CTCGTTTCTC CTAAGCCTGT CGCTGAGTAT 
TCTGCAGTAT ATAGGTTCAG TCGAGCATGT ATCCTAACTG GTGGATTGGG GGTTGATGGA 
GTTAGTCAAG CCCAAGCCAA CTTTCTTCTC CCTGATATGA ATAGACTCGC ATTGGAGGCA 
AAATCAGGAT CACTCGCTAT CTTGTTTATC AGCTTTGCTG GTGCGCAAAA TTCTCAATTT 
GGCATTGATT CAGGCAAGAT TCATTCAGGA AATATAGGAG GACATTGTTT ATGGAGCAAA 
ATACCTCTGC AATCACTGTA TGCGTCGTGG CAGAAATCAC CAAACATGGA CTTGGGACAG 
AGAGTAGACA CAGTCTCTCT TGTTGAAATG CAGCCTTGCT TCATAAAGCT AAAGTCCATG 
AGTGAGGAAA AGTGTGTCTC GATTCAGGTG CCCAGCAATC CACTCACCTC GAGCTCTCCG 
CAGCAAGTGC AAGTCACCAT ATCTGCAGAA GAAGTTGGGT CAACGGAAAA ATCTCCTTAT 
AGTTCATTTT CATATAATGA CATCTCTTCC TCTTCCTTGT TGCAAATTAT CAGGTTGAGA 
ACAGGAAATG TAGTTTTCAA CTACAGATAC TATAACAACA AATTGCAGAA GACTGAAGTA 
ACTGAAGACT TTTCTTGTCC ATTCTGCTTA GTAAAATGTG CCAGTTTCAA GGGCCTGAGA 
TATCACTTGC CATCAACCCA CGATCTCCTC AATTTCGAGT TTTGGGTAAC TGAAGAATTT 
CAGGCGGTAA ATGTCTCCCT CAAGACTGAG ACAATGATAT CCAAGGTTAA TGAGGATGAC 
GTTGACCCAA AGCAGCAAAC TTTCTTTTTT TCACGGAGGA GGCAAAAGAG TCAGGTACGG 
AGCTCAAGGC AAGGGCCTCA TCTTGGATTA GGTTGCGAGG TGCTAGATAA GACTGATGAT 
GCTCATTCTG TTAGAAGTGA GAAGAGCCGA ATACCACCTG GAAAGCATTA CGAAAGAATT 
GGGGGTGCTG AGTCTGGTCA AAGAGTTCCT CCTGGCACGA GTCCTGCAGA CGTGCAATCA 
TGTGGGGATC CAGATTATGT GCAGTCGATA GCTGGAAGTA CAATGTTGCA GTTTGCAAAA 
ACGAGGAAAA TATCTATAGA ACGGTCGGAC TTGAGGAACC GAAGCCTCCT TCAGAAGAGA 
CAGTTCTTCC ACTCTCATCG AGCTCAGCCC ATGGCTCTAG AACAAGTACT TTCGGACCGG 
GATAGTGAAG ATGAAGTTGA TGATGATGTG GCAGATTTTG AAGATAGAAG GATGCTCGAT 
GATTTCGTTG ATGTGACTAA AGATGAGAAA CAGATGATGC ACATGTGGAA CTCGTTTGTG 
AGGAAGCAGC GAGTATTAGC AGATGGTCAC ATTCCATGGG CATGCGAGGC ATTCTCAAGA 
TTGCACGGAC CCATCATGGT TCGAACACCG CACTTGATTT GGTGCTGGAG AGTGTTTATG 
GTGAAACTGT GGAACCACGG TCTTCTTGAT GCCCGAACCA TGAACAACTG TAATACCTTT 
CTCGAACAGC TCCAAATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_199936.2
CDS	312..2192
Translation	MPGIPLVSRETSSCSRSTEQMCHEDSRLRISEEEEIAAEESLAAYCKPVELYNIIQRRAIRNPLFLQRCLHYKIEAKHKRRIQMTVFLSGAIDAGVQTQKLFPLYILLARLVSPKPVAEYSAVYRFSRACILTGGLGVDGVSQAQANFLLPDMNRLALEAKSGSLAILFISFAGAQNSQFGIDSGKIHSGNIGGHCLWSKIPLQSLYASWQKSPNMDLGQRVDTVSLVEMQPCFIKLKSMSEEKCVSIQVPSNPLTSSSPQQVQVTISAEEVGSTEKSPYSSFSYNDISSSSLLQIIRLRTGNVVFNYRYYNNKLQKTEVTEDFSCPFCLVKCASFKGLRYHLPSTHDLLNFEFWVTEEFQAVNVSLKTETMISKVNEDDVDPKQQTFFFSRRRQKSQVRSSRQGPHLGLGCEVLDKTDDAHSVRSEKSRIPPGKHYERIGGAESGQRVPPGTSPADVQSCGDPDYVQSIAGSTMLQFAKTRKISIERSDLRNRSLLQKRQFFHSHRAQPMALEQVLSDRDSEDEVDDDVADFEDRRMLDDFVDVTKDEKQMMHMWNSFVRKQRVLADGHIPWACEAFSRLHGPIMVRTPHLIWCWRVFMVKLWNHGLLDARTMNNCNTFLEQLQI

Target ORF information:

RefSeq Version	NM_124502.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana VEFS-Box of polycomb protein (EMF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_124502.3

ORF Insert Sequence:

ATGCCAGGCA TTCCTCTTGT TAGTCGTGAA ACCTCTTCTT GTTCAAGAAG CACAGAGCAG 
ATGTGCCATG AAGACTCCCG TCTGCGTATT TCGGAAGAGG AGGAGATTGC TGCTGAAGAG 
AGCTTGGCTG CCTATTGCAA GCCTGTTGAA CTCTACAATA TCATTCAACG CCGTGCTATT 
AGGAATCCCT TGTTTCTTCA GCGATGTTTG CATTATAAGA TTGAGGCAAA ACATAAAAGG 
AGAATACAAA TGACTGTATT CCTCTCGGGC GCTATAGATG CTGGGGTACA AACTCAAAAA 
TTATTCCCTC TGTATATTTT GTTGGCAAGA CTCGTTTCTC CTAAGCCTGT CGCTGAGTAT 
TCTGCAGTAT ATAGGTTCAG TCGAGCATGT ATCCTAACTG GTGGATTGGG GGTTGATGGA 
GTTAGTCAAG CCCAAGCCAA CTTTCTTCTC CCTGATATGA ATAGACTCGC ATTGGAGGCA 
AAATCAGGAT CACTCGCTAT CTTGTTTATC AGCTTTGCTG GTGCGCAAAA TTCTCAATTT 
GGCATTGATT CAGGCAAGAT TCATTCAGGA AATATAGGAG GACATTGTTT ATGGAGCAAA 
ATACCTCTGC AATCACTGTA TGCGTCGTGG CAGAAATCAC CAAACATGGA CTTGGGACAG 
AGAGTAGACA CAGTCTCTCT TGTTGAAATG CAGCCTTGCT TCATAAAGCT AAAGTCCATG 
AGTGAGGAAA AGTGTGTCTC GATTCAGGTG CCCAGCAATC CACTCACCTC GAGCTCTCCG 
CAGCAAGTGC AAGTCACCAT ATCTGCAGAA GAAGTTGGGT CAACGGAAAA ATCTCCTTAT 
AGTTCATTTT CATATAATGA CATCTCTTCC TCTTCCTTGT TGCAAATTAT CAGGTTGAGA 
ACAGGAAATG TAGTTTTCAA CTACAGATAC TATAACAACA AATTGCAGAA GACTGAAGTA 
ACTGAAGACT TTTCTTGTCC ATTCTGCTTA GTAAAATGTG CCAGTTTCAA GGGCCTGAGA 
TATCACTTGC CATCAACCCA CGATCTCCTC AATTTCGAGT TTTGGGTAAC TGAAGAATTT 
CAGGCGGTAA ATGTCTCCCT CAAGACTGAG ACAATGATAT CCAAGGTTAA TGAGGATGAC 
GTTGACCCAA AGCAGCAAAC TTTCTTTTTT TCACGGAGGA GGCAAAAGAG TCAGGTACGG 
AGCTCAAGGC AAGGGCCTCA TCTTGGATTA GGTTGCGAGG TGCTAGATAA GACTGATGAT 
GCTCATTCTG TTAGAAGTGA GAAGAGCCGA ATACCACCTG GAAAGCATTA CGAAAGAATT 
GGGGGTGCTG AGTCTGGTCA AAGAGTTCCT CCTGGCACGA GTCCTGCAGA CGTGCAATCA 
TGTGGGGATC CAGATTATGT GCAGTCGATA GCTGGAAGTA CAATGTTGCA GTTTGCAAAA 
ACGAGGAAAA TATCTATAGA ACGGTCGGAC TTGAGGAACC GAAGCCTCCT TCAGAAGAGA 
CAGTTCTTCC ACTCTCATCG AGCTCAGCCC ATGGCTCTAG AACAAGTACT TTCGGACCGG 
GATAGTGAAG ATGAAGTTGA TGATGATGTG GCAGATTTTG AAGATAGAAG GATGCTCGAT 
GATTTCGTTG ATGTGACTAA AGATGAGAAA CAGATGATGC ACATGTGGAA CTCGTTTGTG 
AGGAAGCAGC GAGTATTAGC AGATGGTCAC ATTCCATGGG CATGCGAGGC ATTCTCAAGA 
TTGCACGGAC CCATCATGGT TCGAACACCG CACTTGATTT GGTGCTGGAG AGTGTTTATG 
GTGAAACTGT GGAACCACGG TCTTCTTGAT GCCCGAACCA TGAACAACTG TAATACCTTT 
CTCGAACAGC TCCAAATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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EMF2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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