PWD cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	PWD
Entrez Gene ID	832706
Full Name	chloroplastidic phosphoglucan, water dikinase (ATGWD3)
Synonyms	ATGWD3,F9D12.1,F9D12_1,OK1,PHOSPHOGLUCAN WATER DIKINASE
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_122538.5	NP_198009.3	chloroplastidic phosphoglucan, water dikinase (ATGWD3)
NM_001125808.1	NP_001119280.1	chloroplastidic phosphoglucan, water dikinase (ATGWD3)

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os12g0297500	NP_001066613.1
Arabidopsis thaliana (thale cress)	ATGWD3	NP_198009.3

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our systematic study of the three GWDs of A. thaliana demonstrates that cytosolic GWD2, and in part also plastidic PWD, play unexpected roles in proper seed development.
Title: The analysis of the different functions of starch-phosphorylating enzymes during the development of Arabidopsis thaliana plants discloses an unexpected role for the cytosolic isoform GWD2.

The role of alpha-glucan/water dikinase in the development of cold tolerance in conjunction with SEX1 protein is reported.
Title: Starch-related alpha-glucan/water dikinase is involved in the cold-induced development of freezing tolerance in Arabidopsis.

An Arabidopsis thaliana gene encoding a homologue of the potato alpha-glucan, water dikinase GWD, previously known as R1, was identified by screening the Arabidopsis genome and named AtGWD3. [GWD3]
Title: A novel isoform of glucan, water dikinase phosphorylates pre-phosphorylated alpha-glucans and is involved in starch degradation in Arabidopsis.

The expression and characterization of this phosphoglucan,water dikinase in A. thaliana.
Title: Identification of a novel enzyme required for starch metabolism in Arabidopsis leaves. The phosphoglucan, water dikinase.

The following PWD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PWD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb19594	NM_001125808.1 Latest version!	Arabidopsis thaliana chloroplastidic phosphoglucan, water dikinase (ATGWD3) (PWD), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb19593	NM_122538.5 Latest version!	Arabidopsis thaliana chloroplastidic phosphoglucan, water dikinase (ATGWD3) (PWD), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PWD ( NM_001125808.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001119280.1 Arabidopsis thaliana chloroplastidic phosphoglucan, water dikinase (ATGWD3) (PWD), partial mRNA.

Price & Availability↑

CloneID	OAb19594
Clone ID Related Accession (Same CDS sequence)	NM_001125808.1
Accession Version	NM_001125808.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	chloroplastidic phosphoglucan, water dikinase (ATGWD3)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). COMPLETENESS: incomplete on the 5' end.

ATGGAGAGCA TTGGCAGCCA TTGTTGCAGC TCTCCTTTCA CCTTCATCAC TAGAAACTCA 
TCATCATCAC TTCCTAGACT CGTTAACATC ACTCACAGAG TTAATCTCAG CCACCAATCT 
CACCGACTCA GAAACTCCAA TTCTCGTCTC ACTTGCACTG CTACTTCTTC TTCCACCATT 
GAGGAACAAC GGAAGAAGAA AGATGGGTCA GGAACGAAAG TGAGGTTGAA TGTGAGGTTA 
GATCATCAAG TTAATTTTGG TGACCATGTG GCTATGTTTG GATCAGCTAA AGAGATTGGT 
TCATGGAAAA AGAAATCGCC TTTGAATTGG AGTGAGAATG GATGGGTTTG TGAGTTGGAA 
CTTGACGGTG GTCAGGTTTT GGAGTATAAG TTTGTCATTG TTAAGAATGA TGGTTCACTT 
TCATGGGAAT CTGGTGATAA TCGTGTCCTT AAGGTTCCAA ATTCTGGGAA TTTTTCTGTT 
GTTTGTCATT GGGATGCTAC TAGAGAAACC CTTGATTTGC CTCAGGAGGT TGGTAATGAT 
GATGATGTTG GTGATGGTGG GCATGAGAGG GATAATCATG ATGTTGGTGA TGATAGAGTA 
GTGGGAAGTG AAAATGGTGC GCAGCTTCAG AAGAGTACAT TGGGTGGGCA ATGGCAAGGT 
AAAGATGCGT CCTTTATGCG TTCTAATGAT CATGGTAACA GAGAAGTTGG TAGAAATTGG 
GATACTAGTG GTCTTGAAGG CACAGCTCTT AAGATGGTTG AGGGTGATCG CAACTCTAAG 
AACTGGTGGA GAAAGCTTGA AATGGTACGC GAGGTTATAG TTGGGAGTGT TGAGAGGGAG 
GAACGATTGA AGGCGCTCAT ATACTCTGCA ATTTATTTGA AGTGGATAAA CACAGGTCAG 
ATTCCTTGTT TTGAAGATGG AGGGCATCAC CGTCCAAACA GGCATGCCGA GATTTCCAGA 
CTTATATTCC GTGAGTTGGA GCACATTTGC AGTAAGAAAG ATGCTACTCC AGAGGAAGTG 
CTTGTTGCTC GGAAAATCCA TCCGTGTTTA CCTTCTTTCA AAGCAGAGTT TACTGCAGCT 
GTCCCTCTAA CTCGGATTAG GGACATAGCC CATCGCAATG ATATTCCTCA TGATCTCAAG 
CAAGAAATCA AGCATACGAT ACAAAATAAG CTTCACCGGA ATGCTGGTCC AGAAGATCTA 
ATTGCAACAG AAGCAATGCT TCAACGAATT ACCGAGACCC CAGGAAAATA TAGTGGAGAC 
TTTGTGGAGC AGTTTAAAAT ATTCCATAAT GAGCTTAAAG ATTTCTTTAA TGCTGGAAGT 
CTCACTGAAC AGCTTGATTC TATGAAAATT TCTATGGATG ATAGAGGTCT TTCTGCGCTC 
AATTTGTTTT TTGAATGTAA AAAGCGCCTT GACACATCAG GAGAATCAAG CAATGTTTTG 
GAGTTGATTA AAACCATGCA TTCTCTAGCT TCTTTAAGAG AAACAATTAT AAAGGAACTT 
AATAGCGGCT TGCGAAATGA TGCTCCTGAT ACTGCCATTG CAATGCGCCA GAAGTGGCGC 
CTTTGTGAGA TCGGCCTCGA GGACTACTTT TTTGTTCTAC TAAGCAGATT CCTCAATGCT 
CTTGAAACTA TGGGAGGAGC TGATCAACTG GCAAAAGATG TGGGATCAAG AAACGTTGCC 
TCATGGAATG ATCCACTAGA TGCTTTGGTG TTGGGTGTTC ACCAAGTAGG TCTATCTGGT 
TGGAAGCAAG AAGAATGTTT AGCCATTGGA AATGAACTCC TTGCTTGGCG AGAAAGGGAC 
CTACTTGAAA AAGAAGGGGA AGAGGATGGA AAAACAATTT GGGCCATGAG GCTGAAAGCA 
ACTCTTGATC GAGCACGCAG ATTAACAGCA GAATATTCTG ATTTGCTTCT TCAAATATTT 
CCTCCTAATG TGGAGATTTT AGGAAAAGCT CTAGGAATTC CAGAGAATAG TGTCAAGACC 
TATACAGAAG CAGAGATTCG TGCTGGAATT ATTTTCCAGA TCTCAAAGCT CTGCACTGTT 
CTTCTAAAAG CTGTAAGAAA TTCACTTGGT TCTGAGGGCT GGGATGTCGT TGTACCTGGA 
TCGACGTCTG GGACATTAGT TCAGGTTGAG AGCATTGTTC CGGGATCATT GCCAGCAACT 
TCTGGTGGTC CTATTATTCT CTTGGTCAAT AAAGCTGATG GCGATGAAGA GGTAAGTGCT 
GCTAATGGGA ACATAGCTGG AGTCATGCTT CTGCAGGAGC TGCCTCACTT GTCTCACCTT 
GGCGTTAGAG CGCGGCAGGA GAAAATTGTC TTTGTGACAT GTGATGATGA TGACAAGGTT 
GCTGATATAC GACGACTTGT GGGAAAATTT GTGAGGTTGG AAGCATCTCC AAGTCATGTG 
AATCTGATAC TTTCAACTGA GGGTAGGAGT CGCACTTCCA AATCCAGTGC GACCAAAAAA 
ACGGATAAGA ACAGCTTATC TAAGAAAAAA ACAGATAAGA AGAGCTTATC TATCGATGAT 
GAAGAATCAA AGCCTGGTTC CTCATCTTCC AATAGCCTCC TTTACTCTTC CAAGTGGAGG 
AATCATAGCA CTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001119280.1
CDS	1..2598
Translation	MESIGSHCCSSPFTFITRNSSSSLPRLVNITHRVNLSHQSHRLRNSNSRLTCTATSSSTIEEQRKKKDGSGTKVRLNVRLDHQVNFGDHVAMFGSAKEIGSWKKKSPLNWSENGWVCELELDGGQVLEYKFVIVKNDGSLSWESGDNRVLKVPNSGNFSVVCHWDATRETLDLPQEVGNDDDVGDGGHERDNHDVGDDRVVGSENGAQLQKSTLGGQWQGKDASFMRSNDHGNREVGRNWDTSGLEGTALKMVEGDRNSKNWWRKLEMVREVIVGSVEREERLKALIYSAIYLKWINTGQIPCFEDGGHHRPNRHAEISRLIFRELEHICSKKDATPEEVLVARKIHPCLPSFKAEFTAAVPLTRIRDIAHRNDIPHDLKQEIKHTIQNKLHRNAGPEDLIATEAMLQRITETPGKYSGDFVEQFKIFHNELKDFFNAGSLTEQLDSMKISMDDRGLSALNLFFECKKRLDTSGESSNVLELIKTMHSLASLRETIIKELNSGLRNDAPDTAIAMRQKWRLCEIGLEDYFFVLLSRFLNALETMGGADQLAKDVGSRNVASWNDPLDALVLGVHQVGLSGWKQEECLAIGNELLAWRERDLLEKEGEEDGKTIWAMRLKATLDRARRLTAEYSDLLLQIFPPNVEILGKALGIPENSVKTYTEAEIRAGIIFQISKLCTVLLKAVRNSLGSEGWDVVVPGSTSGTLVQVESIVPGSLPATSGGPIILLVNKADGDEEVSAANGNIAGVMLLQELPHLSHLGVRARQEKIVFVTCDDDDKVADIRRLVGKFVRLEASPSHVNLILSTEGRSRTSKSSATKKTDKNSLSKKKTDKKSLSIDDEESKPGSSSSNSLLYSSKWRNHSTC

Target ORF information:

RefSeq Version	NM_001125808.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana chloroplastidic phosphoglucan, water dikinase (ATGWD3) (PWD), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001125808.1

ORF Insert Sequence:

ATGGAGAGCA TTGGCAGCCA TTGTTGCAGC TCTCCTTTCA CCTTCATCAC TAGAAACTCA 
TCATCATCAC TTCCTAGACT CGTTAACATC ACTCACAGAG TTAATCTCAG CCACCAATCT 
CACCGACTCA GAAACTCCAA TTCTCGTCTC ACTTGCACTG CTACTTCTTC TTCCACCATT 
GAGGAACAAC GGAAGAAGAA AGATGGGTCA GGAACGAAAG TGAGGTTGAA TGTGAGGTTA 
GATCATCAAG TTAATTTTGG TGACCATGTG GCTATGTTTG GATCAGCTAA AGAGATTGGT 
TCATGGAAAA AGAAATCGCC TTTGAATTGG AGTGAGAATG GATGGGTTTG TGAGTTGGAA 
CTTGACGGTG GTCAGGTTTT GGAGTATAAG TTTGTCATTG TTAAGAATGA TGGTTCACTT 
TCATGGGAAT CTGGTGATAA TCGTGTCCTT AAGGTTCCAA ATTCTGGGAA TTTTTCTGTT 
GTTTGTCATT GGGATGCTAC TAGAGAAACC CTTGATTTGC CTCAGGAGGT TGGTAATGAT 
GATGATGTTG GTGATGGTGG GCATGAGAGG GATAATCATG ATGTTGGTGA TGATAGAGTA 
GTGGGAAGTG AAAATGGTGC GCAGCTTCAG AAGAGTACAT TGGGTGGGCA ATGGCAAGGT 
AAAGATGCGT CCTTTATGCG TTCTAATGAT CATGGTAACA GAGAAGTTGG TAGAAATTGG 
GATACTAGTG GTCTTGAAGG CACAGCTCTT AAGATGGTTG AGGGTGATCG CAACTCTAAG 
AACTGGTGGA GAAAGCTTGA AATGGTACGC GAGGTTATAG TTGGGAGTGT TGAGAGGGAG 
GAACGATTGA AGGCGCTCAT ATACTCTGCA ATTTATTTGA AGTGGATAAA CACAGGTCAG 
ATTCCTTGTT TTGAAGATGG AGGGCATCAC CGTCCAAACA GGCATGCCGA GATTTCCAGA 
CTTATATTCC GTGAGTTGGA GCACATTTGC AGTAAGAAAG ATGCTACTCC AGAGGAAGTG 
CTTGTTGCTC GGAAAATCCA TCCGTGTTTA CCTTCTTTCA AAGCAGAGTT TACTGCAGCT 
GTCCCTCTAA CTCGGATTAG GGACATAGCC CATCGCAATG ATATTCCTCA TGATCTCAAG 
CAAGAAATCA AGCATACGAT ACAAAATAAG CTTCACCGGA ATGCTGGTCC AGAAGATCTA 
ATTGCAACAG AAGCAATGCT TCAACGAATT ACCGAGACCC CAGGAAAATA TAGTGGAGAC 
TTTGTGGAGC AGTTTAAAAT ATTCCATAAT GAGCTTAAAG ATTTCTTTAA TGCTGGAAGT 
CTCACTGAAC AGCTTGATTC TATGAAAATT TCTATGGATG ATAGAGGTCT TTCTGCGCTC 
AATTTGTTTT TTGAATGTAA AAAGCGCCTT GACACATCAG GAGAATCAAG CAATGTTTTG 
GAGTTGATTA AAACCATGCA TTCTCTAGCT TCTTTAAGAG AAACAATTAT AAAGGAACTT 
AATAGCGGCT TGCGAAATGA TGCTCCTGAT ACTGCCATTG CAATGCGCCA GAAGTGGCGC 
CTTTGTGAGA TCGGCCTCGA GGACTACTTT TTTGTTCTAC TAAGCAGATT CCTCAATGCT 
CTTGAAACTA TGGGAGGAGC TGATCAACTG GCAAAAGATG TGGGATCAAG AAACGTTGCC 
TCATGGAATG ATCCACTAGA TGCTTTGGTG TTGGGTGTTC ACCAAGTAGG TCTATCTGGT 
TGGAAGCAAG AAGAATGTTT AGCCATTGGA AATGAACTCC TTGCTTGGCG AGAAAGGGAC 
CTACTTGAAA AAGAAGGGGA AGAGGATGGA AAAACAATTT GGGCCATGAG GCTGAAAGCA 
ACTCTTGATC GAGCACGCAG ATTAACAGCA GAATATTCTG ATTTGCTTCT TCAAATATTT 
CCTCCTAATG TGGAGATTTT AGGAAAAGCT CTAGGAATTC CAGAGAATAG TGTCAAGACC 
TATACAGAAG CAGAGATTCG TGCTGGAATT ATTTTCCAGA TCTCAAAGCT CTGCACTGTT 
CTTCTAAAAG CTGTAAGAAA TTCACTTGGT TCTGAGGGCT GGGATGTCGT TGTACCTGGA 
TCGACGTCTG GGACATTAGT TCAGGTTGAG AGCATTGTTC CGGGATCATT GCCAGCAACT 
TCTGGTGGTC CTATTATTCT CTTGGTCAAT AAAGCTGATG GCGATGAAGA GGTAAGTGCT 
GCTAATGGGA ACATAGCTGG AGTCATGCTT CTGCAGGAGC TGCCTCACTT GTCTCACCTT 
GGCGTTAGAG CGCGGCAGGA GAAAATTGTC TTTGTGACAT GTGATGATGA TGACAAGGTT 
GCTGATATAC GACGACTTGT GGGAAAATTT GTGAGGTTGG AAGCATCTCC AAGTCATGTG 
AATCTGATAC TTTCAACTGA GGGTAGGAGT CGCACTTCCA AATCCAGTGC GACCAAAAAA 
ACGGATAAGA ACAGCTTATC TAAGAAAAAA ACAGATAAGA AGAGCTTATC TATCGATGAT 
GAAGAATCAA AGCCTGGTTC CTCATCTTCC AATAGCCTCC TTTACTCTTC CAAGTGGAGG 
AATCATAGCA CTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PWD ( NM_122538.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_198009.3 Arabidopsis thaliana chloroplastidic phosphoglucan, water dikinase (ATGWD3) (PWD), mRNA.

Price & Availability↑

CloneID	OAb19593
Clone ID Related Accession (Same CDS sequence)	NM_122538.5
Accession Version	NM_122538.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	chloroplastidic phosphoglucan, water dikinase (ATGWD3)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_122538.4.

ATGGAGAGCA TTGGCAGCCA TTGTTGCAGC TCTCCTTTCA CCTTCATCAC TAGAAACTCA 
TCATCATCAC TTCCTAGACT CGTTAACATC ACTCACAGAG TTAATCTCAG CCACCAATCT 
CACCGACTCA GAAACTCCAA TTCTCGTCTC ACTTGCACTG CTACTTCTTC TTCCACCATT 
GAGGAACAAC GGAAGAAGAA AGATGGGTCA GGAACGAAAG TGAGGTTGAA TGTGAGGTTA 
GATCATCAAG TTAATTTTGG TGACCATGTG GCTATGTTTG GATCAGCTAA AGAGATTGGT 
TCATGGAAAA AGAAATCGCC TTTGAATTGG AGTGAGAATG GATGGGTTTG TGAGTTGGAA 
CTTGACGGTG GTCAGGTTTT GGAGTATAAG TTTGTCATTG TTAAGAATGA TGGTTCACTT 
TCATGGGAAT CTGGTGATAA TCGTGTCCTT AAGGTTCCAA ATTCTGGGAA TTTTTCTGTT 
GTTTGTCATT GGGATGCTAC TAGAGAAACC CTTGATTTGC CTCAGGAGGT TGGTAATGAT 
GATGATGTTG GTGATGGTGG GCATGAGAGG GATAATCATG ATGTTGGTGA TGATAGAGTA 
GTGGGAAGTG AAAATGGTGC GCAGCTTCAG AAGAGTACAT TGGGTGGGCA ATGGCAAGGT 
AAAGATGCGT CCTTTATGCG TTCTAATGAT CATGGTAACA GAGAAGTTGG TAGAAATTGG 
GATACTAGTG GTCTTGAAGG CACAGCTCTT AAGATGGTTG AGGGTGATCG CAACTCTAAG 
AACTGGTGGA GAAAGCTTGA AATGGTACGC GAGGTTATAG TTGGGAGTGT TGAGAGGGAG 
GAACGATTGA AGGCGCTCAT ATACTCTGCA ATTTATTTGA AGTGGATAAA CACAGGTCAG 
ATTCCTTGTT TTGAAGATGG AGGGCATCAC CGTCCAAACA GGCATGCCGA GATTTCCAGA 
CTTATATTCC GTGAGTTGGA GCACATTTGC AGTAAGAAAG ATGCTACTCC AGAGGAAGTG 
CTTGTTGCTC GGAAAATCCA TCCGTGTTTA CCTTCTTTCA AAGCAGAGTT TACTGCAGCT 
GTCCCTCTAA CTCGGATTAG GGACATAGCC CATCGCAATG ATATTCCTCA TGATCTCAAG 
CAAGAAATCA AGCATACGAT ACAAAATAAG CTTCACCGGA ATGCTGGTCC AGAAGATCTA 
ATTGCAACAG AAGCAATGCT TCAACGAATT ACCGAGACCC CAGGAAAATA TAGTGGAGAC 
TTTGTGGAGC AGTTTAAAAT ATTCCATAAT GAGCTTAAAG ATTTCTTTAA TGCTGGAAGT 
CTCACTGAAC AGCTTGATTC TATGAAAATT TCTATGGATG ATAGAGGTCT TTCTGCGCTC 
AATTTGTTTT TTGAATGTAA AAAGCGCCTT GACACATCAG GAGAATCAAG CAATGTTTTG 
GAGTTGATTA AAACCATGCA TTCTCTAGCT TCTTTAAGAG AAACAATTAT AAAGGAACTT 
AATAGCGGCT TGCGAAATGA TGCTCCTGAT ACTGCCATTG CAATGCGCCA GAAGTGGCGC 
CTTTGTGAGA TCGGCCTCGA GGACTACTTT TTTGTTCTAC TAAGCAGATT CCTCAATGCT 
CTTGAAACTA TGGGAGGAGC TGATCAACTG GCAAAAGATG TGGGATCAAG AAACGTTGCC 
TCATGGAATG ATCCACTAGA TGCTTTGGTG TTGGGTGTTC ACCAAGTAGG TCTATCTGGT 
TGGAAGCAAG AAGAATGTTT AGCCATTGGA AATGAACTCC TTGCTTGGCG AGAAAGGGAC 
CTACTTGAAA AAGAAGGGGA AGAGGATGGA AAAACAATTT GGGCCATGAG GCTGAAAGCA 
ACTCTTGATC GAGCACGCAG ATTAACAGCA GAATATTCTG ATTTGCTTCT TCAAATATTT 
CCTCCTAATG TGGAGATTTT AGGAAAAGCT CTAGGAATTC CAGAGAATAG TGTCAAGACC 
TATACAGAAG CAGAGATTCG TGCTGGAATT ATTTTCCAGA TCTCAAAGCT CTGCACTGTT 
CTTCTAAAAG CTGTAAGAAA TTCACTTGGT TCTGAGGGCT GGGATGTCGT TGTACCTGGA 
TCGACGTCTG GGACATTAGT TCAGGTTGAG AGCATTGTTC CGGGATCATT GCCAGCAACT 
TCTGGTGGTC CTATTATTCT CTTGGTCAAT AAAGCTGATG GCGATGAAGA GGTAAGTGCT 
GCTAATGGGA ACATAGCTGG AGTCATGCTT CTGCAGGAGC TGCCTCACTT GTCTCACCTT 
GGCGTTAGAG CGCGGCAGGA GAAAATTGTC TTTGTGACAT GTGATGATGA TGACAAGGTT 
GCTGATATAC GACGACTTGT GGGAAAATTT GTGAGGTTGG AAGCATCTCC AAGTCATGTG 
AATCTGATAC TTTCAACTGA GGGTAGGAGT CGCACTTCCA AATCCAGTGC GACCAAAAAA 
ACGGATAAGA ACAGCTTATC TAAGAAAAAA ACAGATAAGA AGAGCTTATC TATCGATGAT 
GAAGAATCAA AGCCTGGTTC CTCATCTTCC AATAGCCTCC TTTACTCTTC CAAGGATATC 
CCTAGTGGAG GAATCATAGC ACTTGCTGAT GCAGATGTAC CAACTTCTGG TTCAAAATCT 
GCTGCATGTG GTCTTCTTGC ATCTTTAGCA GAAGCCTCTA GTAAAGTGCA CAGCGAACAC 
GGAGTTCCGG CATCATTTAA GGTTCCAACT GGAGTTGTCA TACCTTTTGG ATCGATGGAA 
TTAGCTTTAA AGCAAAATAA TTCGGAAGAA AAGTTTGCGT CTTTGCTAGA AAAACTAGAA 
ACCGCCAGAC CTGAGGGTGG TGAGCTAGAC GACATATGTG ACCAGATCCA TGAAGTGATG 
AAAACGTTGC AAGTGCCTAA AGAAACAATC AACAGCATAA GCAAAGCGTT TCTCAAAGAT 
GCTCGTCTCA TTGTTCGTTC AAGTGCTAAC GTCGAGGACT TAGCCGGAAT GTCAGCTGCA 
GGACTCTATG AATCAATCCC TAACGTGAGT CCCTCGGATC CTTTGGTGTT TTCAGATTCG 
GTTTGCCAAG TTTGGGCTTC TCTCTACACA AGAAGAGCTG TTCTAAGCCG TAGAGCTGCT 
GGTGTCTCTC AAAGAGAAGC TTCAATGGCT GTTCTCGTTC AAGAAATGCT TTCGCCGGAC 
TTATCATTCG TTCTGCACAC AGTGAGTCCA GCTGATCCGG ACAGTAACCT TGTGGAAGCC 
GAGATCGCTC CTGGTTTAGG TGAGACTTTA GCTTCAGGAA CAAGAGGAAC ACCATGGAGA 
CTCGCTTCGG GTAAGCTCGA CGGGATTGTA CAAACCTTAG CTTTCGCAAA CTTCAGCGAA 
GAGCTTCTTG TGTCAGGAAC AGGTCCTGCT GATGGAAAAT ACGTTCGGTT GACCGTGGAC 
TATAGCAAAA AACGTTTAAC TGTTGACTCG GTGTTTAGAC AGCAGCTCGG TCAGAGACTC 
GGTTCGGTTG GTTTCTTCTT GGAAAGAAAC TTTGGCTGTG CTCAAGACGT TGAAGGTTGT 
TTGGTTGGTG AAGATGTTTA CATTGTTCAG TCAAGGCCAC AACCTCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_198009.3
CDS	102..3692
Translation	MESIGSHCCSSPFTFITRNSSSSLPRLVNITHRVNLSHQSHRLRNSNSRLTCTATSSSTIEEQRKKKDGSGTKVRLNVRLDHQVNFGDHVAMFGSAKEIGSWKKKSPLNWSENGWVCELELDGGQVLEYKFVIVKNDGSLSWESGDNRVLKVPNSGNFSVVCHWDATRETLDLPQEVGNDDDVGDGGHERDNHDVGDDRVVGSENGAQLQKSTLGGQWQGKDASFMRSNDHGNREVGRNWDTSGLEGTALKMVEGDRNSKNWWRKLEMVREVIVGSVEREERLKALIYSAIYLKWINTGQIPCFEDGGHHRPNRHAEISRLIFRELEHICSKKDATPEEVLVARKIHPCLPSFKAEFTAAVPLTRIRDIAHRNDIPHDLKQEIKHTIQNKLHRNAGPEDLIATEAMLQRITETPGKYSGDFVEQFKIFHNELKDFFNAGSLTEQLDSMKISMDDRGLSALNLFFECKKRLDTSGESSNVLELIKTMHSLASLRETIIKELNSGLRNDAPDTAIAMRQKWRLCEIGLEDYFFVLLSRFLNALETMGGADQLAKDVGSRNVASWNDPLDALVLGVHQVGLSGWKQEECLAIGNELLAWRERDLLEKEGEEDGKTIWAMRLKATLDRARRLTAEYSDLLLQIFPPNVEILGKALGIPENSVKTYTEAEIRAGIIFQISKLCTVLLKAVRNSLGSEGWDVVVPGSTSGTLVQVESIVPGSLPATSGGPIILLVNKADGDEEVSAANGNIAGVMLLQELPHLSHLGVRARQEKIVFVTCDDDDKVADIRRLVGKFVRLEASPSHVNLILSTEGRSRTSKSSATKKTDKNSLSKKKTDKKSLSIDDEESKPGSSSSNSLLYSSKDIPSGGIIALADADVPTSGSKSAACGLLASLAEASSKVHSEHGVPASFKVPTGVVIPFGSMELALKQNNSEEKFASLLEKLETARPEGGELDDICDQIHEVMKTLQVPKETINSISKAFLKDARLIVRSSANVEDLAGMSAAGLYESIPNVSPSDPLVFSDSVCQVWASLYTRRAVLSRRAAGVSQREASMAVLVQEMLSPDLSFVLHTVSPADPDSNLVEAEIAPGLGETLASGTRGTPWRLASGKLDGIVQTLAFANFSEELLVSGTGPADGKYVRLTVDYSKKRLTVDSVFRQQLGQRLGSVGFFLERNFGCAQDVEGCLVGEDVYIVQSRPQPL

Target ORF information:

RefSeq Version	NM_122538.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana chloroplastidic phosphoglucan, water dikinase (ATGWD3) (PWD), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_122538.5

ORF Insert Sequence:

ATGGAGAGCA TTGGCAGCCA TTGTTGCAGC TCTCCTTTCA CCTTCATCAC TAGAAACTCA 
TCATCATCAC TTCCTAGACT CGTTAACATC ACTCACAGAG TTAATCTCAG CCACCAATCT 
CACCGACTCA GAAACTCCAA TTCTCGTCTC ACTTGCACTG CTACTTCTTC TTCCACCATT 
GAGGAACAAC GGAAGAAGAA AGATGGGTCA GGAACGAAAG TGAGGTTGAA TGTGAGGTTA 
GATCATCAAG TTAATTTTGG TGACCATGTG GCTATGTTTG GATCAGCTAA AGAGATTGGT 
TCATGGAAAA AGAAATCGCC TTTGAATTGG AGTGAGAATG GATGGGTTTG TGAGTTGGAA 
CTTGACGGTG GTCAGGTTTT GGAGTATAAG TTTGTCATTG TTAAGAATGA TGGTTCACTT 
TCATGGGAAT CTGGTGATAA TCGTGTCCTT AAGGTTCCAA ATTCTGGGAA TTTTTCTGTT 
GTTTGTCATT GGGATGCTAC TAGAGAAACC CTTGATTTGC CTCAGGAGGT TGGTAATGAT 
GATGATGTTG GTGATGGTGG GCATGAGAGG GATAATCATG ATGTTGGTGA TGATAGAGTA 
GTGGGAAGTG AAAATGGTGC GCAGCTTCAG AAGAGTACAT TGGGTGGGCA ATGGCAAGGT 
AAAGATGCGT CCTTTATGCG TTCTAATGAT CATGGTAACA GAGAAGTTGG TAGAAATTGG 
GATACTAGTG GTCTTGAAGG CACAGCTCTT AAGATGGTTG AGGGTGATCG CAACTCTAAG 
AACTGGTGGA GAAAGCTTGA AATGGTACGC GAGGTTATAG TTGGGAGTGT TGAGAGGGAG 
GAACGATTGA AGGCGCTCAT ATACTCTGCA ATTTATTTGA AGTGGATAAA CACAGGTCAG 
ATTCCTTGTT TTGAAGATGG AGGGCATCAC CGTCCAAACA GGCATGCCGA GATTTCCAGA 
CTTATATTCC GTGAGTTGGA GCACATTTGC AGTAAGAAAG ATGCTACTCC AGAGGAAGTG 
CTTGTTGCTC GGAAAATCCA TCCGTGTTTA CCTTCTTTCA AAGCAGAGTT TACTGCAGCT 
GTCCCTCTAA CTCGGATTAG GGACATAGCC CATCGCAATG ATATTCCTCA TGATCTCAAG 
CAAGAAATCA AGCATACGAT ACAAAATAAG CTTCACCGGA ATGCTGGTCC AGAAGATCTA 
ATTGCAACAG AAGCAATGCT TCAACGAATT ACCGAGACCC CAGGAAAATA TAGTGGAGAC 
TTTGTGGAGC AGTTTAAAAT ATTCCATAAT GAGCTTAAAG ATTTCTTTAA TGCTGGAAGT 
CTCACTGAAC AGCTTGATTC TATGAAAATT TCTATGGATG ATAGAGGTCT TTCTGCGCTC 
AATTTGTTTT TTGAATGTAA AAAGCGCCTT GACACATCAG GAGAATCAAG CAATGTTTTG 
GAGTTGATTA AAACCATGCA TTCTCTAGCT TCTTTAAGAG AAACAATTAT AAAGGAACTT 
AATAGCGGCT TGCGAAATGA TGCTCCTGAT ACTGCCATTG CAATGCGCCA GAAGTGGCGC 
CTTTGTGAGA TCGGCCTCGA GGACTACTTT TTTGTTCTAC TAAGCAGATT CCTCAATGCT 
CTTGAAACTA TGGGAGGAGC TGATCAACTG GCAAAAGATG TGGGATCAAG AAACGTTGCC 
TCATGGAATG ATCCACTAGA TGCTTTGGTG TTGGGTGTTC ACCAAGTAGG TCTATCTGGT 
TGGAAGCAAG AAGAATGTTT AGCCATTGGA AATGAACTCC TTGCTTGGCG AGAAAGGGAC 
CTACTTGAAA AAGAAGGGGA AGAGGATGGA AAAACAATTT GGGCCATGAG GCTGAAAGCA 
ACTCTTGATC GAGCACGCAG ATTAACAGCA GAATATTCTG ATTTGCTTCT TCAAATATTT 
CCTCCTAATG TGGAGATTTT AGGAAAAGCT CTAGGAATTC CAGAGAATAG TGTCAAGACC 
TATACAGAAG CAGAGATTCG TGCTGGAATT ATTTTCCAGA TCTCAAAGCT CTGCACTGTT 
CTTCTAAAAG CTGTAAGAAA TTCACTTGGT TCTGAGGGCT GGGATGTCGT TGTACCTGGA 
TCGACGTCTG GGACATTAGT TCAGGTTGAG AGCATTGTTC CGGGATCATT GCCAGCAACT 
TCTGGTGGTC CTATTATTCT CTTGGTCAAT AAAGCTGATG GCGATGAAGA GGTAAGTGCT 
GCTAATGGGA ACATAGCTGG AGTCATGCTT CTGCAGGAGC TGCCTCACTT GTCTCACCTT 
GGCGTTAGAG CGCGGCAGGA GAAAATTGTC TTTGTGACAT GTGATGATGA TGACAAGGTT 
GCTGATATAC GACGACTTGT GGGAAAATTT GTGAGGTTGG AAGCATCTCC AAGTCATGTG 
AATCTGATAC TTTCAACTGA GGGTAGGAGT CGCACTTCCA AATCCAGTGC GACCAAAAAA 
ACGGATAAGA ACAGCTTATC TAAGAAAAAA ACAGATAAGA AGAGCTTATC TATCGATGAT 
GAAGAATCAA AGCCTGGTTC CTCATCTTCC AATAGCCTCC TTTACTCTTC CAAGGATATC 
CCTAGTGGAG GAATCATAGC ACTTGCTGAT GCAGATGTAC CAACTTCTGG TTCAAAATCT 
GCTGCATGTG GTCTTCTTGC ATCTTTAGCA GAAGCCTCTA GTAAAGTGCA CAGCGAACAC 
GGAGTTCCGG CATCATTTAA GGTTCCAACT GGAGTTGTCA TACCTTTTGG ATCGATGGAA 
TTAGCTTTAA AGCAAAATAA TTCGGAAGAA AAGTTTGCGT CTTTGCTAGA AAAACTAGAA 
ACCGCCAGAC CTGAGGGTGG TGAGCTAGAC GACATATGTG ACCAGATCCA TGAAGTGATG 
AAAACGTTGC AAGTGCCTAA AGAAACAATC AACAGCATAA GCAAAGCGTT TCTCAAAGAT 
GCTCGTCTCA TTGTTCGTTC AAGTGCTAAC GTCGAGGACT TAGCCGGAAT GTCAGCTGCA 
GGACTCTATG AATCAATCCC TAACGTGAGT CCCTCGGATC CTTTGGTGTT TTCAGATTCG 
GTTTGCCAAG TTTGGGCTTC TCTCTACACA AGAAGAGCTG TTCTAAGCCG TAGAGCTGCT 
GGTGTCTCTC AAAGAGAAGC TTCAATGGCT GTTCTCGTTC AAGAAATGCT TTCGCCGGAC 
TTATCATTCG TTCTGCACAC AGTGAGTCCA GCTGATCCGG ACAGTAACCT TGTGGAAGCC 
GAGATCGCTC CTGGTTTAGG TGAGACTTTA GCTTCAGGAA CAAGAGGAAC ACCATGGAGA 
CTCGCTTCGG GTAAGCTCGA CGGGATTGTA CAAACCTTAG CTTTCGCAAA CTTCAGCGAA 
GAGCTTCTTG TGTCAGGAAC AGGTCCTGCT GATGGAAAAT ACGTTCGGTT GACCGTGGAC 
TATAGCAAAA AACGTTTAAC TGTTGACTCG GTGTTTAGAC AGCAGCTCGG TCAGAGACTC 
GGTTCGGTTG GTTTCTTCTT GGAAAGAAAC TTTGGCTGTG CTCAAGACGT TGAAGGTTGT 
TTGGTTGGTG AAGATGTTTA CATTGTTCAG TCAAGGCCAC AACCTCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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PWD cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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