GASA4 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	GASA4
Entrez Gene ID	831375
Full Name	GAST1 protein homolog 4
Synonyms	F8M21.120,F8M21_120,GAST1 protein homolog 4
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_121527.4	NP_197027.1	GAST1 protein homolog 4
NM_001085118.2	NP_001078587.1	GAST1 protein homolog 4

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	GASA4	NP_197027.1
Arabidopsis thaliana (thale cress)	AT1G74670	NP_177605.2

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the N-terminal signal peptide is cleaved as predicted, and the cleavage is important for proper sub-cellular localization of GASA4 and GASA6.
Title: Molecular and cellular characterization of GA-Stimulated Transcripts GASA4 and GASA6 in Arabidopsis thaliana.

GASA4 over-expression promotes germination and flowering, and inhibits H2O2 accumulation in leaves following wounding.
Title: The Arabidopsis cysteine-rich protein GASA4 promotes GA responses and exhibits redox activity in bacteria and in planta.

functionally characterized the GASA4 gene and found that heat induced GASA4 expression.
Title: Enhanced tolerance to heat stress in transgenic plants expressing the GASA4 gene.

Phenotypic analyses of GASA4 loss-of-function and gain-of-function lines indicate that GASA4 regulates floral meristem identity and also positively affects both seed size and total seed yield.
Title: GASA4, one of the 14-member Arabidopsis GASA family of small polypeptides, regulates flowering and seed development.

The following GASA4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GASA4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb18463	NM_121527.4 Latest version!	Arabidopsis thaliana GAST1 protein homolog 4 (GASA4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OAb18464	NM_001085118.2 Latest version!	Arabidopsis thaliana GAST1 protein homolog 4 (GASA4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GASA4 ( NM_121527.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_197027.1 Arabidopsis thaliana GAST1 protein homolog 4 (GASA4), mRNA.

Price & Availability↑

CloneID	OAb18463
Clone ID Related Accession (Same CDS sequence)	NM_121527.4
Accession Version	NM_121527.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	GAST1 protein homolog 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_121527.3.

ATGGCTAAGT CATATGGAGC TATCTTCCTC TTGACCCTCA TTGTCCTCTT CATGCTTCAA 
ACCATGGTTA TGGCCTCAAG TGGATCTAAT GTGAAGTGGA GCCAGAAACG TTATGGACCA 
GGAAGCCTGA AACGTACCCA ATGCCCATCG GAATGTGATA GGAGGTGTAA AAAGACACAG 
TACCACAAGG CTTGCATTAC GTTCTGCAAC AAATGCTGCA GGAAGTGTCT CTGTGTGCCT 
CCGGGTTACT ATGGGAACAA ACAAGTTTGC TCCTGCTACA ACAACTGGAA AACTCAAGAG 
GGTGGACCAA AATGCCCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_197027.1
CDS	202..522
Translation	MAKSYGAIFLLTLIVLFMLQTMVMASSGSNVKWSQKRYGPGSLKRTQCPSECDRRCKKTQYHKACITFCNKCCRKCLCVPPGYYGNKQVCSCYNNWKTQEGGPKCP

Target ORF information:

RefSeq Version	NM_121527.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana GAST1 protein homolog 4 (GASA4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_121527.4

ORF Insert Sequence:

ATGGCTAAGT CATATGGAGC TATCTTCCTC TTGACCCTCA TTGTCCTCTT CATGCTTCAA 
ACCATGGTTA TGGCCTCAAG TGGATCTAAT GTGAAGTGGA GCCAGAAACG TTATGGACCA 
GGAAGCCTGA AACGTACCCA ATGCCCATCG GAATGTGATA GGAGGTGTAA AAAGACACAG 
TACCACAAGG CTTGCATTAC GTTCTGCAAC AAATGCTGCA GGAAGTGTCT CTGTGTGCCT 
CCGGGTTACT ATGGGAACAA ACAAGTTTGC TCCTGCTACA ACAACTGGAA AACTCAAGAG 
GGTGGACCAA AATGCCCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GASA4 ( NM_001085118.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001078587.1 Arabidopsis thaliana GAST1 protein homolog 4 (GASA4), mRNA.

Price & Availability↑

CloneID	OAb18464
Clone ID Related Accession (Same CDS sequence)	NM_001085118.2
Accession Version	NM_001085118.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	GAST1 protein homolog 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_001085118.1.

ATGGCCTCAA GTGGATCTAA TGTGAAGTGG AGCCAGAAAC GTTATGGACC AGGAAGCCTG 
AAACGTACCC AATGCCCATC GGAATGTGAT AGGAGGTGTA AAAAGACACA GTACCACAAG 
GCTTGCATTA CGTTCTGCAA CAAATGCTGC AGGAAGTGTC TCTGTGTGCC TCCGGGTTAC 
TATGGGAACA AACAAGTTTG CTCCTGCTAC AACAACTGGA AAACTCAAGA GGGTGGACCA 
AAATGCCCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001078587.1
CDS	225..476
Translation	MASSGSNVKWSQKRYGPGSLKRTQCPSECDRRCKKTQYHKACITFCNKCCRKCLCVPPGYYGNKQVCSCYNNWKTQEGGPKCP

Target ORF information:

RefSeq Version	NM_001085118.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana GAST1 protein homolog 4 (GASA4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001085118.2

ORF Insert Sequence:

ATGGCCTCAA GTGGATCTAA TGTGAAGTGG AGCCAGAAAC GTTATGGACC AGGAAGCCTG 
AAACGTACCC AATGCCCATC GGAATGTGAT AGGAGGTGTA AAAAGACACA GTACCACAAG 
GCTTGCATTA CGTTCTGCAA CAAATGCTGC AGGAAGTGTC TCTGTGTGCC TCCGGGTTAC 
TATGGGAACA AACAAGTTTG CTCCTGCTAC AACAACTGGA AAACTCAAGA GGGTGGACCA 
AAATGCCCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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GASA4 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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