DRM2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	DRM2
Entrez Gene ID	831315
Full Name	domains rearranged methyltransferase 2
Synonyms	AT5G14630,DMT7,NA METHYLTRANSFERASE,T15N1.110,T15N1_110,domains rearranged methyltransferase 2
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_121466.2	NP_196966.2	DNA (cytosine-5)-methyltransferase DRM2
NM_121466.3	NP_196966.2	domains rearranged methyltransferase 2

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	DRM2	NP_196966.2
Arabidopsis thaliana (thale cress)	DRM1	NP_197042.2
Oryza sativa (rice)	Os11g0109200	NP_001176307.1
Oryza sativa (rice)	Os12g0108900	NP_001065954.1
Oryza sativa (rice)	Os03g0110800	NP_001048718.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CHH methylation in the egg cell depends on DOMAINS REARRANGED METHYLASE 2 (DRM2) and RNA polymerase V (Pol V), two main actors of RNA-directed DNA methylation, but does not depend on Pol IV
Title: Live-cell analysis of DNA methylation during sexual reproduction in Arabidopsis reveals context and sex-specific dynamics controlled by noncanonical RdDM.

DRM2 gene expression throughout normal development in Arabidopsis
Title: [Developmental expression of Arabidopsis methyltransferase genes MET1, DRM2 and CMT3].

Study shows that Arabidopsis DRM2 exists in complex with the small interfering RNA (siRNA) effector ARGONAUTE4 (AGO4) and preferentially methylates one DNA strand, likely the strand acting as the template for RNA polymerase V-mediated noncoding RNA transcripts.
Title: Molecular mechanism of action of plant DRM de novo DNA methyltransferases.

These findings substantiate the view that DRM2 is the major de novo DNA methyltransferase in the RdDM pathway and support a direct catalytic role for this enzyme in RdDM.
Title: Genetic evidence that DNA methyltransferase DRM2 has a direct catalytic role in RNA-directed DNA methylation in Arabidopsis thaliana.

Data show that by site-directed mutagenesis that both the DRM2 N-terminal UBA domains and C-terminal methyltransferase domain are required for normal RNA-directed DNA methylation, supporting an essential targeting function for the UBA domains.
Title: The de novo cytosine methyltransferase DRM2 requires intact UBA domains and a catalytically mutated paralog DRM3 during RNA-directed DNA methylation in Arabidopsis thaliana.

The following DRM2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DRM2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb18404	NM_121466.3 Latest version!	Arabidopsis thaliana domains rearranged methyltransferase 2 (DRM2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
OAb18404	NM_121466.2	Arabidopsis thaliana DNA (cytosine-5)-methyltransferase DRM2 mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DRM2 ( NM_121466.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_196966.2 Arabidopsis thaliana DNA (cytosine-5)-methyltransferase DRM2 mRNA, complete cds.

Price & Availability↑

CloneID	OAb18404
Clone ID Related Accession (Same CDS sequence)	NM_121466.2 , NM_121466.3
Accession Version	NM_121466.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-01-21
Organism	Arabidopsis thaliana(Thale cress)
Product	DNA (cytosine-5)-methyltransferase DRM2
Comment

ATGGTGATTT GGAATAACGA TGATGATGAT TTTTTGGAGA TTGATAACTT TCAATCTTCT 
CCACGGTCAT CTCCAATACA TGCAATGCAG TGTAGGGTCG AAAATCTAGC TGGTGTAGCC 
GTGACAACTA GTTCTTTGAG CTCTCCTACT GAGACAACTG ATTTAGTTCA GATGGGCTTC 
TCAGACGAGG TTTTTGCTAC ATTGTTTGAC ATGGGATTTC CTGTTGAGAT GATTTCTAGA 
GCGATCAAGG AAACTGGACC AAATGTAGAA ACTTCGGTTA TAATTGATAC TATCTCCAAA 
TACTCAAGCG ACTGTGAAGC TGGTTCTTCC AAGTCCAAGG CTATTGATCA TTTCCTTGCT 
ATGGGATTTG ATGAAGAAAA AGTTGTCAAA GCCATTCAAG AACATGGAGA AGACAATATG 
GAAGCAATTG CAAATGCATT GCTCTCTTGT CCAGAGGCTA AGAAACTGCC AGCAGCAGTA 
GAGGAAGAAG ATGGCATTGA CTGGTCATCA AGTGATGATG ATACCAATTA CACCGATATG 
TTAAACTCAG ATGATGAGAA AGATCCAAAC TCAAATGAAA ATGGCAGCAA AATACGGTCT 
TTGGTGAAGA TGGGTTTCTC AGAGCTTGAA GCTTCTTTAG CTGTCGAGAG ATGTGGAGAA 
AATGTGGATA TTGCAGAGCT CACAGACTTC CTTTGTGCTG CTCAAATGGC TAGGGAATTT 
AGTGAGTTTT ACACTGAACA TGAAGAACAA AAGCCTAGAC ATAATATTAA GAAAAGGCGG 
TTTGAGTCAA AAGGAGAGCC AAGATCATCT GTTGATGACG AGCCGATTCG TCTACCAAAT 
CCAATGATAG GATTTGGGGT TCCAAACGAG CCCGGACTCA TTACACATAG ATCGCTTCCA 
GAGTTAGCCC GAGGGCCACC TTTTTTCTAC TATGAGAATG TCGCCCTCAC ACCTAAAGGC 
GTTTGGGAGA CTATTTCCAG GCACTTGTTC GAGATCCCAC CTGAGTTTGT GGACTCAAAA 
TATTTCTGTG TTGCAGCGAG GAAGAGAGGC TACATCCACA ATCTCCCCAT CAACAACAGA 
TTTCAGATTC AGCCTCCACC AAAATACACC ATCCATGATG CATTTCCTTT GAGTAAGAGA 
TGGTGGCCAG AATGGGATAA AAGGACCAAG CTTAATTGCA TTTTGACTTG TACAGGTAGT 
GCTCAGTTGA CTAACAGGAT TCGTGTAGCC CTTGAGCCTT ACAATGAAGA ACCAGAACCG 
CCTAAGCATG TACAAAGATA TGTGATTGAC CAGTGCAAAA AATGGAATTT GGTTTGGGTG 
GGTAAAAACA AAGCTGCCCC ACTCGAGCCA GATGAGATGG AGAGTATTCT GGGATTTCCA 
AAAAATCATA CTCGTGGTGG AGGCATGAGT AGAACTGAGC GCTTCAAGTC CTTAGGAAAT 
TCGTTTCAGG TTGATACTGT GGCGTATCAT CTGTCTGTCC TGAAGCCCAT TTTCCCACAT 
GGAATCAATG TTCTCTCTCT TTTCACGGGT ATTGGTGGTG GGGAAGTGGC ACTTCATCGT 
CTCCAAATCA AAATGAAGCT TGTTGTGTCT GTTGAGATTT CAAAAGTCAA CAGAAATATT 
TTGAAGGACT TTTGGGAGCA AACTAACCAG ACTGGAGAAT TGATCGAGTT TTCAGACATC 
CAACACTTGA CTAATGACAC AATCGAAGGG TTGATGGAGA AATATGGTGG ATTTGATCTT 
GTAATTGGAG GAAGTCCTTG TAACAATCTG GCAGGCGGTA ATAGGGTAAG CCGAGTTGGT 
CTTGAAGGTG ATCAATCTTC GTTGTTCTTT GAGTATTGCC GTATTCTAGA GGTGGTACGT 
GCGAGGATGA GAGGATCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_196966.2

Translation

MVIWNNDDDDFLEIDNFQSSPRSSPIHAMQCRVENLAGVAVTTSSLSSPTETTDLVQMGFSDEVFATLFDMGFPVEMISRAIKETGPNVETSVIIDTISKYSSDCEAGSSKSKAIDHFLAMGFDEEKVVKAIQEHGEDNMEAIANALLSCPEAKKLPAAVEEEDGIDWSSSDDDTNYTDMLNSDDEKDPNSNENGSKIRSLVKMGFSELEASLAVERCGENVDIAELTDFLCAAQMAREFSEFYTEHEEQKPRHNIKKRRFESKGEPRSSVDDEPIRLPNPMIGFGVPNEPGLITHRSLPELARGPPFFYYENVALTPKGVWETISRHLFEIPPEFVDSKYFCVAARKRGYIHNLPINNRFQIQPPPKYTIHDAFPLSKRWWPEWDKRTKLNCILTCTGSAQLTNRIRVALEPYNEEPEPPKHVQRYVIDQCKKWNLVWVGKNKAAPLEPDEMESILGFPKNHTRGGGMSRTERFKSLGNSFQVDTVAYHLSVLKPIFPHGINVLSLFTGIGGGEVALHRLQIKMKLVVSVEISKVNRNILKDFWEQTNQTGELIEFSDIQHLTNDTIEGLMEKYGGFDLVIGGSPCNNLAGGNRVSRVGLEGDQSSLFFEYCRILEVVRARMRGS

Target ORF information:

RefSeq Version	NM_121466.2
Organism	Arabidopsis thaliana(Thale cress)
Definition	Arabidopsis thaliana DNA (cytosine-5)-methyltransferase DRM2 mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_121466.2

ORF Insert Sequence:

ATGGTGATTT GGAATAACGA TGATGATGAT TTTTTGGAGA TTGATAACTT TCAATCTTCT 
CCACGGTCAT CTCCAATACA TGCAATGCAG TGTAGGGTCG AAAATCTAGC TGGTGTAGCC 
GTGACAACTA GTTCTTTGAG CTCTCCTACT GAGACAACTG ATTTAGTTCA GATGGGCTTC 
TCAGACGAGG TTTTTGCTAC ATTGTTTGAC ATGGGATTTC CTGTTGAGAT GATTTCTAGA 
GCGATCAAGG AAACTGGACC AAATGTAGAA ACTTCGGTTA TAATTGATAC TATCTCCAAA 
TACTCAAGCG ACTGTGAAGC TGGTTCTTCC AAGTCCAAGG CTATTGATCA TTTCCTTGCT 
ATGGGATTTG ATGAAGAAAA AGTTGTCAAA GCCATTCAAG AACATGGAGA AGACAATATG 
GAAGCAATTG CAAATGCATT GCTCTCTTGT CCAGAGGCTA AGAAACTGCC AGCAGCAGTA 
GAGGAAGAAG ATGGCATTGA CTGGTCATCA AGTGATGATG ATACCAATTA CACCGATATG 
TTAAACTCAG ATGATGAGAA AGATCCAAAC TCAAATGAAA ATGGCAGCAA AATACGGTCT 
TTGGTGAAGA TGGGTTTCTC AGAGCTTGAA GCTTCTTTAG CTGTCGAGAG ATGTGGAGAA 
AATGTGGATA TTGCAGAGCT CACAGACTTC CTTTGTGCTG CTCAAATGGC TAGGGAATTT 
AGTGAGTTTT ACACTGAACA TGAAGAACAA AAGCCTAGAC ATAATATTAA GAAAAGGCGG 
TTTGAGTCAA AAGGAGAGCC AAGATCATCT GTTGATGACG AGCCGATTCG TCTACCAAAT 
CCAATGATAG GATTTGGGGT TCCAAACGAG CCCGGACTCA TTACACATAG ATCGCTTCCA 
GAGTTAGCCC GAGGGCCACC TTTTTTCTAC TATGAGAATG TCGCCCTCAC ACCTAAAGGC 
GTTTGGGAGA CTATTTCCAG GCACTTGTTC GAGATCCCAC CTGAGTTTGT GGACTCAAAA 
TATTTCTGTG TTGCAGCGAG GAAGAGAGGC TACATCCACA ATCTCCCCAT CAACAACAGA 
TTTCAGATTC AGCCTCCACC AAAATACACC ATCCATGATG CATTTCCTTT GAGTAAGAGA 
TGGTGGCCAG AATGGGATAA AAGGACCAAG CTTAATTGCA TTTTGACTTG TACAGGTAGT 
GCTCAGTTGA CTAACAGGAT TCGTGTAGCC CTTGAGCCTT ACAATGAAGA ACCAGAACCG 
CCTAAGCATG TACAAAGATA TGTGATTGAC CAGTGCAAAA AATGGAATTT GGTTTGGGTG 
GGTAAAAACA AAGCTGCCCC ACTCGAGCCA GATGAGATGG AGAGTATTCT GGGATTTCCA 
AAAAATCATA CTCGTGGTGG AGGCATGAGT AGAACTGAGC GCTTCAAGTC CTTAGGAAAT 
TCGTTTCAGG TTGATACTGT GGCGTATCAT CTGTCTGTCC TGAAGCCCAT TTTCCCACAT 
GGAATCAATG TTCTCTCTCT TTTCACGGGT ATTGGTGGTG GGGAAGTGGC ACTTCATCGT 
CTCCAAATCA AAATGAAGCT TGTTGTGTCT GTTGAGATTT CAAAAGTCAA CAGAAATATT 
TTGAAGGACT TTTGGGAGCA AACTAACCAG ACTGGAGAAT TGATCGAGTT TTCAGACATC 
CAACACTTGA CTAATGACAC AATCGAAGGG TTGATGGAGA AATATGGTGG ATTTGATCTT 
GTAATTGGAG GAAGTCCTTG TAACAATCTG GCAGGCGGTA ATAGGGTAAG CCGAGTTGGT 
CTTGAAGGTG ATCAATCTTC GTTGTTCTTT GAGTATTGCC GTATTCTAGA GGTGGTACGT 
GCGAGGATGA GAGGATCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DRM2 ( NM_121466.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_196966.2 Arabidopsis thaliana domains rearranged methyltransferase 2 (DRM2), mRNA.

Price & Availability↑

CloneID	OAb18404
Clone ID Related Accession (Same CDS sequence)	NM_121466.2 , NM_121466.3
Accession Version	NM_121466.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	domains rearranged methyltransferase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_121466.2.

ATGGTGATTT GGAATAACGA TGATGATGAT TTTTTGGAGA TTGATAACTT TCAATCTTCT 
CCACGGTCAT CTCCAATACA TGCAATGCAG TGTAGGGTCG AAAATCTAGC TGGTGTAGCC 
GTGACAACTA GTTCTTTGAG CTCTCCTACT GAGACAACTG ATTTAGTTCA GATGGGCTTC 
TCAGACGAGG TTTTTGCTAC ATTGTTTGAC ATGGGATTTC CTGTTGAGAT GATTTCTAGA 
GCGATCAAGG AAACTGGACC AAATGTAGAA ACTTCGGTTA TAATTGATAC TATCTCCAAA 
TACTCAAGCG ACTGTGAAGC TGGTTCTTCC AAGTCCAAGG CTATTGATCA TTTCCTTGCT 
ATGGGATTTG ATGAAGAAAA AGTTGTCAAA GCCATTCAAG AACATGGAGA AGACAATATG 
GAAGCAATTG CAAATGCATT GCTCTCTTGT CCAGAGGCTA AGAAACTGCC AGCAGCAGTA 
GAGGAAGAAG ATGGCATTGA CTGGTCATCA AGTGATGATG ATACCAATTA CACCGATATG 
TTAAACTCAG ATGATGAGAA AGATCCAAAC TCAAATGAAA ATGGCAGCAA AATACGGTCT 
TTGGTGAAGA TGGGTTTCTC AGAGCTTGAA GCTTCTTTAG CTGTCGAGAG ATGTGGAGAA 
AATGTGGATA TTGCAGAGCT CACAGACTTC CTTTGTGCTG CTCAAATGGC TAGGGAATTT 
AGTGAGTTTT ACACTGAACA TGAAGAACAA AAGCCTAGAC ATAATATTAA GAAAAGGCGG 
TTTGAGTCAA AAGGAGAGCC AAGATCATCT GTTGATGACG AGCCGATTCG TCTACCAAAT 
CCAATGATAG GATTTGGGGT TCCAAACGAG CCCGGACTCA TTACACATAG ATCGCTTCCA 
GAGTTAGCCC GAGGGCCACC TTTTTTCTAC TATGAGAATG TCGCCCTCAC ACCTAAAGGC 
GTTTGGGAGA CTATTTCCAG GCACTTGTTC GAGATCCCAC CTGAGTTTGT GGACTCAAAA 
TATTTCTGTG TTGCAGCGAG GAAGAGAGGC TACATCCACA ATCTCCCCAT CAACAACAGA 
TTTCAGATTC AGCCTCCACC AAAATACACC ATCCATGATG CATTTCCTTT GAGTAAGAGA 
TGGTGGCCAG AATGGGATAA AAGGACCAAG CTTAATTGCA TTTTGACTTG TACAGGTAGT 
GCTCAGTTGA CTAACAGGAT TCGTGTAGCC CTTGAGCCTT ACAATGAAGA ACCAGAACCG 
CCTAAGCATG TACAAAGATA TGTGATTGAC CAGTGCAAAA AATGGAATTT GGTTTGGGTG 
GGTAAAAACA AAGCTGCCCC ACTCGAGCCA GATGAGATGG AGAGTATTCT GGGATTTCCA 
AAAAATCATA CTCGTGGTGG AGGCATGAGT AGAACTGAGC GCTTCAAGTC CTTAGGAAAT 
TCGTTTCAGG TTGATACTGT GGCGTATCAT CTGTCTGTCC TGAAGCCCAT TTTCCCACAT 
GGAATCAATG TTCTCTCTCT TTTCACGGGT ATTGGTGGTG GGGAAGTGGC ACTTCATCGT 
CTCCAAATCA AAATGAAGCT TGTTGTGTCT GTTGAGATTT CAAAAGTCAA CAGAAATATT 
TTGAAGGACT TTTGGGAGCA AACTAACCAG ACTGGAGAAT TGATCGAGTT TTCAGACATC 
CAACACTTGA CTAATGACAC AATCGAAGGG TTGATGGAGA AATATGGTGG ATTTGATCTT 
GTAATTGGAG GAAGTCCTTG TAACAATCTG GCAGGCGGTA ATAGGGTAAG CCGAGTTGGT 
CTTGAAGGTG ATCAATCTTC GTTGTTCTTT GAGTATTGCC GTATTCTAGA GGTGGTACGT 
GCGAGGATGA GAGGATCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_196966.2
CDS	127..2007
Translation	MVIWNNDDDDFLEIDNFQSSPRSSPIHAMQCRVENLAGVAVTTSSLSSPTETTDLVQMGFSDEVFATLFDMGFPVEMISRAIKETGPNVETSVIIDTISKYSSDCEAGSSKSKAIDHFLAMGFDEEKVVKAIQEHGEDNMEAIANALLSCPEAKKLPAAVEEEDGIDWSSSDDDTNYTDMLNSDDEKDPNSNENGSKIRSLVKMGFSELEASLAVERCGENVDIAELTDFLCAAQMAREFSEFYTEHEEQKPRHNIKKRRFESKGEPRSSVDDEPIRLPNPMIGFGVPNEPGLITHRSLPELARGPPFFYYENVALTPKGVWETISRHLFEIPPEFVDSKYFCVAARKRGYIHNLPINNRFQIQPPPKYTIHDAFPLSKRWWPEWDKRTKLNCILTCTGSAQLTNRIRVALEPYNEEPEPPKHVQRYVIDQCKKWNLVWVGKNKAAPLEPDEMESILGFPKNHTRGGGMSRTERFKSLGNSFQVDTVAYHLSVLKPIFPHGINVLSLFTGIGGGEVALHRLQIKMKLVVSVEISKVNRNILKDFWEQTNQTGELIEFSDIQHLTNDTIEGLMEKYGGFDLVIGGSPCNNLAGGNRVSRVGLEGDQSSLFFEYCRILEVVRARMRGS

Target ORF information:

RefSeq Version	NM_121466.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana domains rearranged methyltransferase 2 (DRM2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_121466.3

ORF Insert Sequence:

ATGGTGATTT GGAATAACGA TGATGATGAT TTTTTGGAGA TTGATAACTT TCAATCTTCT 
CCACGGTCAT CTCCAATACA TGCAATGCAG TGTAGGGTCG AAAATCTAGC TGGTGTAGCC 
GTGACAACTA GTTCTTTGAG CTCTCCTACT GAGACAACTG ATTTAGTTCA GATGGGCTTC 
TCAGACGAGG TTTTTGCTAC ATTGTTTGAC ATGGGATTTC CTGTTGAGAT GATTTCTAGA 
GCGATCAAGG AAACTGGACC AAATGTAGAA ACTTCGGTTA TAATTGATAC TATCTCCAAA 
TACTCAAGCG ACTGTGAAGC TGGTTCTTCC AAGTCCAAGG CTATTGATCA TTTCCTTGCT 
ATGGGATTTG ATGAAGAAAA AGTTGTCAAA GCCATTCAAG AACATGGAGA AGACAATATG 
GAAGCAATTG CAAATGCATT GCTCTCTTGT CCAGAGGCTA AGAAACTGCC AGCAGCAGTA 
GAGGAAGAAG ATGGCATTGA CTGGTCATCA AGTGATGATG ATACCAATTA CACCGATATG 
TTAAACTCAG ATGATGAGAA AGATCCAAAC TCAAATGAAA ATGGCAGCAA AATACGGTCT 
TTGGTGAAGA TGGGTTTCTC AGAGCTTGAA GCTTCTTTAG CTGTCGAGAG ATGTGGAGAA 
AATGTGGATA TTGCAGAGCT CACAGACTTC CTTTGTGCTG CTCAAATGGC TAGGGAATTT 
AGTGAGTTTT ACACTGAACA TGAAGAACAA AAGCCTAGAC ATAATATTAA GAAAAGGCGG 
TTTGAGTCAA AAGGAGAGCC AAGATCATCT GTTGATGACG AGCCGATTCG TCTACCAAAT 
CCAATGATAG GATTTGGGGT TCCAAACGAG CCCGGACTCA TTACACATAG ATCGCTTCCA 
GAGTTAGCCC GAGGGCCACC TTTTTTCTAC TATGAGAATG TCGCCCTCAC ACCTAAAGGC 
GTTTGGGAGA CTATTTCCAG GCACTTGTTC GAGATCCCAC CTGAGTTTGT GGACTCAAAA 
TATTTCTGTG TTGCAGCGAG GAAGAGAGGC TACATCCACA ATCTCCCCAT CAACAACAGA 
TTTCAGATTC AGCCTCCACC AAAATACACC ATCCATGATG CATTTCCTTT GAGTAAGAGA 
TGGTGGCCAG AATGGGATAA AAGGACCAAG CTTAATTGCA TTTTGACTTG TACAGGTAGT 
GCTCAGTTGA CTAACAGGAT TCGTGTAGCC CTTGAGCCTT ACAATGAAGA ACCAGAACCG 
CCTAAGCATG TACAAAGATA TGTGATTGAC CAGTGCAAAA AATGGAATTT GGTTTGGGTG 
GGTAAAAACA AAGCTGCCCC ACTCGAGCCA GATGAGATGG AGAGTATTCT GGGATTTCCA 
AAAAATCATA CTCGTGGTGG AGGCATGAGT AGAACTGAGC GCTTCAAGTC CTTAGGAAAT 
TCGTTTCAGG TTGATACTGT GGCGTATCAT CTGTCTGTCC TGAAGCCCAT TTTCCCACAT 
GGAATCAATG TTCTCTCTCT TTTCACGGGT ATTGGTGGTG GGGAAGTGGC ACTTCATCGT 
CTCCAAATCA AAATGAAGCT TGTTGTGTCT GTTGAGATTT CAAAAGTCAA CAGAAATATT 
TTGAAGGACT TTTGGGAGCA AACTAACCAG ACTGGAGAAT TGATCGAGTT TTCAGACATC 
CAACACTTGA CTAATGACAC AATCGAAGGG TTGATGGAGA AATATGGTGG ATTTGATCTT 
GTAATTGGAG GAAGTCCTTG TAACAATCTG GCAGGCGGTA ATAGGGTAAG CCGAGTTGGT 
CTTGAAGGTG ATCAATCTTC GTTGTTCTTT GAGTATTGCC GTATTCTAGA GGTGGTACGT 
GCGAGGATGA GAGGATCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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DRM2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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