IMD1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

The following IMD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IMD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OAb18357 NM_121424.6
Latest version!
Arabidopsis thaliana isopropylmalate dehydrogenase 1 (IMD1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OAb18358 NM_001036802.1
Latest version!
Arabidopsis thaliana isopropylmalate dehydrogenase 1 (IMD1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OAb18359 NM_001036803.1
Latest version!
Arabidopsis thaliana isopropylmalate dehydrogenase 1 (IMD1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OAb18357
    Clone ID Related Accession (Same CDS sequence) NM_121424.6
    Accession Version NM_121424.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1230bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-13
    Organism Arabidopsis thaliana(thale cress)
    Product isopropylmalate dehydrogenase 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_121424.5.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGCGGCGT TTTTGCAAAC GAACATCAGT CTGAATGCGA TCAAGATCGT CCCGGGGAAA 
    TACAGTTCTC TCACCGATCA TCAGTTTCGT GCGCCGTATC GAATTAGGTG CGCCGCCGCT
    TCACCTGGGA AAAAACGGTA TAACATCGCT CTGCTTCCCG GAGACGGTAT CGGTCCAGAA
    GTTATCTCTG TTGCTAAGAA TGTGCTTCAG AAAGCTGGAT CTCTCGAAGG ACTGGAGTTT
    GATTTCAAGG AGATGCCTGT CGGAGGAGCA GCCTTGGATT TGGTTGGAGT TCCATTGCCG
    GAGGAAACTT TCACGGCTGC AAAACTATCT GATGCCATTC TTCTTGGAGC TATTGGAGGG
    TACAAATGGG ACAAGAATGA GAAACATCTG AGACCTGAGA TGGCTCTGTT TTACCTTAGA
    AGAGATCTCA AAGTCTTTGC TAATTTGAGA CCTGCTACAG TTTTGCCACA GCTAGTTGAT
    GCTTCCACAC TGAAGAAAGA AGTAGCAGAA GGTGTTGATA TGATGATTGT TAGGGAGCTC
    ACAGGAGGTA TTTACTTTGG AGAGCCAAGA GGCATTACGA TCAATGAAAA TGGCGAAGAA
    GTCGGCGTTA GTACAGAGAT CTACGCTGCT CACGAGATTG ACAGAATTGC TCGTGTAGCA
    TTCGAGACTG CTAGGAAAAG GCGTGGCAAA CTTTGTTCTG TTGACAAAGC CAATGTGTTG
    GATGCATCAA TATTGTGGAG GAAAAGAGTA ACAGCTTTAG CCTCTGAATA TCCAGATGTT
    GAACTATCAC ATATGTATGT CGATAATGCT GCAATGCAGC TTATTCGTGA CCCGAAACAG
    TTTGACACAA TCGTTACCAA TAACATTTTT GGTGATATAT TGTCTGATGA AGCTTCAATG
    ATCACTGGAA GCATTGGAAT GCTTCCATCT GCTAGTCTCG GTGAATCGGG ACCTGGACTC
    TTTGAACCTA TACATGGTTC AGCACCAGAT ATAGCTGGAC AAGACAAGGC AAACCCATTG
    GCCACCATTC TCAGCGCGGC GATGCTTCTG AAGTATGGAC TTGGAGAAGA AAAGGCTGCA
    AAGAGGATTG AAGACGCTGT CGTGGATGCT CTGAACAAAG GGTTCAGAAC CGGAGACATC
    TACTCCCCTG GAAATAAACT GGTGGGATGC AAGGAAATGG GTGAGGAGGT TCTCAAATCA
    GTGGAATCCA AAGTTCCAGC TACTGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_196924.1
    CDS98..1327
    Translation

    Target ORF information:

    RefSeq Version NM_121424.6
    Organism Arabidopsis thaliana(thale cress)
    Definition Arabidopsis thaliana isopropylmalate dehydrogenase 1 (IMD1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_121424.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGCGGCGT TTTTGCAAAC GAACATCAGT CTGAATGCGA TCAAGATCGT CCCGGGGAAA 
    TACAGTTCTC TCACCGATCA TCAGTTTCGT GCGCCGTATC GAATTAGGTG CGCCGCCGCT
    TCACCTGGGA AAAAACGGTA TAACATCGCT CTGCTTCCCG GAGACGGTAT CGGTCCAGAA
    GTTATCTCTG TTGCTAAGAA TGTGCTTCAG AAAGCTGGAT CTCTCGAAGG ACTGGAGTTT
    GATTTCAAGG AGATGCCTGT CGGAGGAGCA GCCTTGGATT TGGTTGGAGT TCCATTGCCG
    GAGGAAACTT TCACGGCTGC AAAACTATCT GATGCCATTC TTCTTGGAGC TATTGGAGGG
    TACAAATGGG ACAAGAATGA GAAACATCTG AGACCTGAGA TGGCTCTGTT TTACCTTAGA
    AGAGATCTCA AAGTCTTTGC TAATTTGAGA CCTGCTACAG TTTTGCCACA GCTAGTTGAT
    GCTTCCACAC TGAAGAAAGA AGTAGCAGAA GGTGTTGATA TGATGATTGT TAGGGAGCTC
    ACAGGAGGTA TTTACTTTGG AGAGCCAAGA GGCATTACGA TCAATGAAAA TGGCGAAGAA
    GTCGGCGTTA GTACAGAGAT CTACGCTGCT CACGAGATTG ACAGAATTGC TCGTGTAGCA
    TTCGAGACTG CTAGGAAAAG GCGTGGCAAA CTTTGTTCTG TTGACAAAGC CAATGTGTTG
    GATGCATCAA TATTGTGGAG GAAAAGAGTA ACAGCTTTAG CCTCTGAATA TCCAGATGTT
    GAACTATCAC ATATGTATGT CGATAATGCT GCAATGCAGC TTATTCGTGA CCCGAAACAG
    TTTGACACAA TCGTTACCAA TAACATTTTT GGTGATATAT TGTCTGATGA AGCTTCAATG
    ATCACTGGAA GCATTGGAAT GCTTCCATCT GCTAGTCTCG GTGAATCGGG ACCTGGACTC
    TTTGAACCTA TACATGGTTC AGCACCAGAT ATAGCTGGAC AAGACAAGGC AAACCCATTG
    GCCACCATTC TCAGCGCGGC GATGCTTCTG AAGTATGGAC TTGGAGAAGA AAAGGCTGCA
    AAGAGGATTG AAGACGCTGT CGTGGATGCT CTGAACAAAG GGTTCAGAAC CGGAGACATC
    TACTCCCCTG GAAATAAACT GGTGGGATGC AAGGAAATGG GTGAGGAGGT TCTCAAATCA
    GTGGAATCCA AAGTTCCAGC TACTGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OAb18358
    Clone ID Related Accession (Same CDS sequence) NM_001036802.1
    Accession Version NM_001036802.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 903bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-13
    Organism Arabidopsis thaliana(thale cress)
    Product isopropylmalate dehydrogenase 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGCGGCGT TTTTGCAAAC GAACATCAGT CTGAATGCGA TCAAGATCGT CCCGGGGAAA 
    TACAGTTCTC TCACCGATCA TCAGTTTCGT GCGCCGTATC GAATTAGGTG CGCCGCCGCT
    TCACCTGGGA AAAAACGGTA TAACATCGCT CTGCTTCCCG GAGACGGTAT CGGTCCAGAA
    GTTATCTCTG TTGCTAAGAA TGTGCTTCAG AAAGCTGGAT CTCTCGAAGG ACTGGAGTTT
    GATTTCAAGG AGATGCCTGT CGGAGGAGCA GCCTTGGATT TGGTTGGAGT TCCATTGCCG
    GAGGAAACTT TCACGGCTGC AAAACTATCT GATGCCATTC TTCTTGGAGC TATTGGAGGG
    TACAAATGGG ACAAGAATGA GAAACATCTG AGACCTGAGA TGGCTCTGTT TTACCTTAGA
    AGAGATCTCA AAGTCTTTGC TAATTTGAGA CCTGCTACAG TTTTGCCACA GCTAGTTGAT
    GCTTCCACAC TGAAGAAAGA AGTAGCAGAA GGTGTTGATA TGATGATTGT TAGGGAGCTC
    ACAGGAGGTA TTTACTTTGG AGAGCCAAGA GGCATTACGA TCAATGAAAA TGGCGAAGAA
    GTCGGCGTTA GTACAGAGAT CTACGCTGCT CACGAGATTG ACAGAATTGC TCGTGTAGCA
    TTCGAGACTG CTAGGAAAAG GCGTGGCAAA CTTTGTTCTG TTGACAAAGC CAATGTGTTG
    GATGCATCAA TATTGTGGAG GAAAAGAGTA ACAGCTTTAG CCTCTGAATA TCCAGATGTT
    GAACTATCAC ATATGTATGT CGATAATGCT GCAATGCAGC TTATTCGTGA CCCGAAACAG
    GTTTTGACAC AATCGTTACC AATAACATTT TTGGTGATAT ATTGTCTGAT GAAGCTTCAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001031879.1
    CDS19..921
    Translation

    Target ORF information:

    RefSeq Version NM_001036802.1
    Organism Arabidopsis thaliana(thale cress)
    Definition Arabidopsis thaliana isopropylmalate dehydrogenase 1 (IMD1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001036802.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGCGGCGT TTTTGCAAAC GAACATCAGT CTGAATGCGA TCAAGATCGT CCCGGGGAAA 
    TACAGTTCTC TCACCGATCA TCAGTTTCGT GCGCCGTATC GAATTAGGTG CGCCGCCGCT
    TCACCTGGGA AAAAACGGTA TAACATCGCT CTGCTTCCCG GAGACGGTAT CGGTCCAGAA
    GTTATCTCTG TTGCTAAGAA TGTGCTTCAG AAAGCTGGAT CTCTCGAAGG ACTGGAGTTT
    GATTTCAAGG AGATGCCTGT CGGAGGAGCA GCCTTGGATT TGGTTGGAGT TCCATTGCCG
    GAGGAAACTT TCACGGCTGC AAAACTATCT GATGCCATTC TTCTTGGAGC TATTGGAGGG
    TACAAATGGG ACAAGAATGA GAAACATCTG AGACCTGAGA TGGCTCTGTT TTACCTTAGA
    AGAGATCTCA AAGTCTTTGC TAATTTGAGA CCTGCTACAG TTTTGCCACA GCTAGTTGAT
    GCTTCCACAC TGAAGAAAGA AGTAGCAGAA GGTGTTGATA TGATGATTGT TAGGGAGCTC
    ACAGGAGGTA TTTACTTTGG AGAGCCAAGA GGCATTACGA TCAATGAAAA TGGCGAAGAA
    GTCGGCGTTA GTACAGAGAT CTACGCTGCT CACGAGATTG ACAGAATTGC TCGTGTAGCA
    TTCGAGACTG CTAGGAAAAG GCGTGGCAAA CTTTGTTCTG TTGACAAAGC CAATGTGTTG
    GATGCATCAA TATTGTGGAG GAAAAGAGTA ACAGCTTTAG CCTCTGAATA TCCAGATGTT
    GAACTATCAC ATATGTATGT CGATAATGCT GCAATGCAGC TTATTCGTGA CCCGAAACAG
    GTTTTGACAC AATCGTTACC AATAACATTT TTGGTGATAT ATTGTCTGAT GAAGCTTCAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OAb18359
    Clone ID Related Accession (Same CDS sequence) NM_001036803.1
    Accession Version NM_001036803.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1167bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-13
    Organism Arabidopsis thaliana(thale cress)
    Product isopropylmalate dehydrogenase 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGCGGCGT TTTTGCAAAC GAACATCAGT CTGAATGCGA TCAAGATCGT CCCGGGGAAA 
    TACAGTTCTC TCACCGATCA TCAGTTTCGT GCGCCGTATC GAATTAGGTG CGCCGCCGCT
    TCACCTGGGA AAAAACGGTA TAACATCGCT CTGCTTCCCG GAGACGGTAT CGGTCCAGAA
    GTTATCTCTG TTGCTAAGAA TGTGCTTCAG AAAGCTGGAT CTCTCGAAGG ACTGGAGTTT
    GATTTCAAGG AGATGCCTGT CGGAGGAGCA GCCTTGGATT TGGTTGGAGT TCCATTGCCG
    GAGGAAACTT TCACGGCTGC AAAACTATCT GATGCCATTC TTCTTGGAGC TATTGGAGGG
    TACAAATGGG ACAAGAATGA GAAACATCTG AGACCTGAGA TGGCTCTGTT TTACCTTAGA
    AGAGATCTCA AAGTCTTTGC TAATTTGAGA CCTGCTACAG TTTTGCCACA GCTAGTTGAT
    GCTTCCACAC TGAAGAAAGA AGTAGCAGAA GGTGTTGATA TGATGATTGT TAGGGAGCTC
    ACAGGAGGTA TTTACTTTGG AGAGCCAAGA GGCATTACGA TCAATGAAAA TGGCGAAGAA
    GTCGGCGTTA GTACAGAGAT CTACGCTGCT CACGAGATTG ACAGAATTGC TCGTGTAGCA
    TTCGAGACTG CTAGGAAAAG GCGTGGCAAA CTTTGTTCTG TTGACAAAGC CAATGTGTTG
    GATGCATCAA TATTGTGGAG GAAAAGAGTA ACAGCTTTAG CCTCTGAATA TCCAGATGTT
    GAACTATCAC ATATGTATGT CGATAATGCT GCAATGCAGC TTATTCGTGA CCCGAAACAG
    TTTGACACAA TCGTTACCAA TAACATTTTT GGTGATATAT TGTCTGATGA AGCTTCAATG
    ATCACTGGAA GCATTGGAAT GCTTCCATCT GCTAGTCTCG GTGAATCGGG ACCTGGACTC
    TTTGAACCTA TACATGGTTC AGCACCAGAT ATAGCTGGAC AAGACAAGGC AAACCCATTG
    GCCACCATTC TCAGCGCGGC GATGCTTCTG AAGTATGGAC TTGGAGAAGA AAAGGCTGCA
    AAGAGGATTG AAGACGCTGT CGTGGATGCT CTGAACAAAG GGTTCAGAAC CGGAGACATC
    TACTCCCCTG GAAATGTATG TGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001031880.1
    CDS19..1185
    Translation

    Target ORF information:

    RefSeq Version NM_001036803.1
    Organism Arabidopsis thaliana(thale cress)
    Definition Arabidopsis thaliana isopropylmalate dehydrogenase 1 (IMD1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001036803.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGCGGCGT TTTTGCAAAC GAACATCAGT CTGAATGCGA TCAAGATCGT CCCGGGGAAA 
    TACAGTTCTC TCACCGATCA TCAGTTTCGT GCGCCGTATC GAATTAGGTG CGCCGCCGCT
    TCACCTGGGA AAAAACGGTA TAACATCGCT CTGCTTCCCG GAGACGGTAT CGGTCCAGAA
    GTTATCTCTG TTGCTAAGAA TGTGCTTCAG AAAGCTGGAT CTCTCGAAGG ACTGGAGTTT
    GATTTCAAGG AGATGCCTGT CGGAGGAGCA GCCTTGGATT TGGTTGGAGT TCCATTGCCG
    GAGGAAACTT TCACGGCTGC AAAACTATCT GATGCCATTC TTCTTGGAGC TATTGGAGGG
    TACAAATGGG ACAAGAATGA GAAACATCTG AGACCTGAGA TGGCTCTGTT TTACCTTAGA
    AGAGATCTCA AAGTCTTTGC TAATTTGAGA CCTGCTACAG TTTTGCCACA GCTAGTTGAT
    GCTTCCACAC TGAAGAAAGA AGTAGCAGAA GGTGTTGATA TGATGATTGT TAGGGAGCTC
    ACAGGAGGTA TTTACTTTGG AGAGCCAAGA GGCATTACGA TCAATGAAAA TGGCGAAGAA
    GTCGGCGTTA GTACAGAGAT CTACGCTGCT CACGAGATTG ACAGAATTGC TCGTGTAGCA
    TTCGAGACTG CTAGGAAAAG GCGTGGCAAA CTTTGTTCTG TTGACAAAGC CAATGTGTTG
    GATGCATCAA TATTGTGGAG GAAAAGAGTA ACAGCTTTAG CCTCTGAATA TCCAGATGTT
    GAACTATCAC ATATGTATGT CGATAATGCT GCAATGCAGC TTATTCGTGA CCCGAAACAG
    TTTGACACAA TCGTTACCAA TAACATTTTT GGTGATATAT TGTCTGATGA AGCTTCAATG
    ATCACTGGAA GCATTGGAAT GCTTCCATCT GCTAGTCTCG GTGAATCGGG ACCTGGACTC
    TTTGAACCTA TACATGGTTC AGCACCAGAT ATAGCTGGAC AAGACAAGGC AAACCCATTG
    GCCACCATTC TCAGCGCGGC GATGCTTCTG AAGTATGGAC TTGGAGAAGA AAAGGCTGCA
    AAGAGGATTG AAGACGCTGT CGTGGATGCT CTGAACAAAG GGTTCAGAAC CGGAGACATC
    TACTCCCCTG GAAATGTATG TGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
    Nature408(6814)823-6(2000 Dec)
    Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,