KEG cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	KEG
Entrez Gene ID	831197
Full Name	protein kinases;ubiquitin-protein ligase
Synonyms	KEEP ON GOING,T6I14.60,T6I14_60
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_121356.3	NP_196857.2	protein kinases;ubiquitin-protein ligase
NM_001203374.1	NP_001190303.1	protein kinases;ubiquitin-protein ligase

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ANKK1	XP_546521.2
Bos taurus (cattle)	ANKK1	XP_003586985.1
Mus musculus (house mouse)	Ankk1	NP_766510.1
Arabidopsis thaliana (thale cress)	KEG	NP_196857.2
Pan troglodytes (chimpanzee)	ANKK1	XP_003952091.1
Rattus norvegicus (Norway rat)	Ankk1	XP_006243085.1
Danio rerio (zebrafish)	ankk1	NP_001124137.1
Xenopus tropicalis (tropical clawed frog)	ankk1	XP_002937872.1
Homo sapiens (human)	ANKK1	NP_848605.1
Gallus gallus (chicken)	ANKK1	XP_003642663.2

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

KEG is an important factor in plant hormone signaling and a positive regulator of JAZ12 stability.
Title: The RING E3 Ligase KEEP ON GOING Modulates JASMONATE ZIM-DOMAIN12 Stability.

ABF1 and ABF3 abundance is affected by ABA and KEG
Title: ABA and the ubiquitin E3 ligase KEEP ON GOING affect proteolysis of the Arabidopsis thaliana transcription factors ABF1 and ABF3.

CIPK26(AT5G21326) interacts with KEG is degraded by the ubiquitin-proteasome system.
Title: Arabidopsis CIPK26 interacts with KEG, components of the ABA signalling network and is degraded by the ubiquitin-proteasome system.

Data indicate that KEEP ON GOING (KEG) E3 interacts with abscisic acid insensitive 5 (ABI5) in the cytoplasm.
Title: Cytoplasmic degradation of the Arabidopsis transcription factor abscisic acid insensitive 5 is mediated by the RING-type E3 ligase KEEP ON GOING.

KEG is a key component of trans-Golgi network and early endosomes (TGN/EE) that regulates post-Golgi trafficking events, including vacuole biogenesis, targeting of membrane-associated proteins to the vacuole, and secretion of apoplastic proteins.
Title: The KEEP ON GOING protein of Arabidopsis regulates intracellular protein trafficking and is degraded during fungal infection.

The following KEG gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KEG cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb18297	NM_001203374.1 Latest version!	Arabidopsis thaliana protein kinases;ubiquitin-protein ligase (KEG), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OAb18298	NM_121356.3 Latest version!	Arabidopsis thaliana protein kinases;ubiquitin-protein ligase (KEG), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KEG ( NM_001203374.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001190303.1 Arabidopsis thaliana protein kinases;ubiquitin-protein ligase (KEG), mRNA.

Price & Availability↑

CloneID	OAb18297
Clone ID Related Accession (Same CDS sequence)	NM_001203374.1
Accession Version	NM_001203374.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	protein kinases;ubiquitin-protein ligase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGGTTGGGA GAGTCAAGGT CCCTTGTTGT TCCGTGTGCC ACACGCGGTA TAATGAAGAT 
GAGAGAGTTC CTTTATTGCT TCAATGCGGC CATGGCTTCT GCAAAGATTG TCTTTCCAAG 
ATGTTCTCTA CTTCTTCCGA CACAACCCTC ACTTGTCCCC GGTGTCGCCA CGTGTCTGTT 
GTTGGGAACT CTGTTCAGGG TCTCAGGAAG AACTACGCTA TGCTTGCTCT CATTCACGCC 
GCATCCGGCG GCGCTAACTT CGATTGCGAT TACACGGATG ATGAGGATGA CGACGATGAA 
GAAGATGGTA GTGATGAAGA TGGAGCTCGT GCCGCTCGTG GATTTCATGC CTCAAGCTCG 
ATTAATAGCT TGTGTGGTCC AGTGATCGAG GTCGGAGCTC ACCCGGAGAT GAAGCTGGTG 
AGGCAGATTG GGGAAGAGAG TAGTAGTGGT GGTTTTGGAG GTGTGGAGAT GTGGGATGCA 
ACTGTTGCAG GCGGTGGTGG ACGGTGTAAG CACCGTGTGG CGGTGAAAAA GATGACTTTG 
ACGGAGGACA TGGATGTTGA GTGGATGCAG GGACAGCTTG AGAGCTTGCG GAGAGCATCC 
ATGTGGTGTA GGAATGTTTG TACTTTCCAT GGGGTTGTGA AGATGGATGG ATCTCTTTGT 
CTTTTGATGG ACAGGTGCTT TGGGTCTGTT CAGTCTGAGA TGCAACGCAA TGAAGGCAGA 
CTTACTTTGG AGCAGATTCT AAGATATGGG GCTGATGTTG CTCGAGGGGT TGCAGAACTC 
CATGCAGCAG GTGTTATCTG TATGAATATA AAACCTTCCA ATCTTCTTTT GGATGCAAGC 
GGCAATGCTG TGGTTTCTGA TTATGGGCTC GCTCCCATCC TAAAGAAGCC AACATGCCAG 
AAAACTCGAC CAGAGTTTGA TTCCTCAAAA GTCACTCTAT ACACGGACTG TGTAACACTC 
AGTCCACACT ACACTGCCCC AGAGGCTTGG GGACCGGTGA AAAAACTTTT TTGGGAGGAT 
GCAAGTGGTG TATCTCCAGA ATCAGATGCA TGGAGTTTTG GCTGTACGTT AGTAGAGATG 
TGCACTGGAT CCACTCCGTG GGATGGTCTT AGTAGAGAAG AAATTTTTCA AGCTGTTGTG 
AAAGCCCGAA AAGTACCACC ACAGTATGAA CGCATAGTGG GTGTGGGAAT TCCTCGGGAA 
TTATGGAAAA TGATTGGTGA ATGTCTTCAG TTTAAACCGT CGAAAAGACC AACCTTTAAT 
GCAATGCTTG CTACATTTCT TCGTCATCTA CAAGAGATAC CACGTAGCCC TTCAGCAAGC 
CCTGATAATG GGATTGCCAA AATATGTGAA GTAAACATTG TACAAGCACC TCGCGCGACT 
AACATAGGGG TCTTTCAGGA TAACCCTAAC AACCTACATC GGGTTGTTTT GGAGGGAGAC 
TTTGAGGGTG TAAGAAATAT ACTTGCAAAG GCTGCAGCTG GAGGCGGTGG AAGCTCTGTG 
CGATCTCTAT TAGAGGCGCA AAACGCTGAT GGTCAATCGG CTCTTCACTT AGCTTGCAGA 
CGTGGCAGTG CTGAACTTGT GGAGGCTATA CTGGAGTATG GGGAGGCTAA TGTAGATATC 
GTGGACAAAG ATGGGGATCC TCCTCTTGTC TTTGCTTTAG CAGCTGGATC TCCACAATGT 
GTTCACGTTC TCATTAAAAA GGGGGCTAAT GTGAGGTCCA GACTGCGGGA AGGATCTGGC 
CCATCTGTAG CTCACGTGTG TTCGTACCAT GGCCAACCTG ATTGTATGCG TGAATTGCTG 
GTAGCTGGAG CCGATCCAAA TGCAGTGGAT GATGAAGGTG AAACTGTGCT TCATCGAGCT 
GTTGCCAAGA AATACACAGA TTGTGCCATT GTTATATTGG AAAATGGGGG ATCTAGATCA 
ATGACTGTTT CAAATGCGAA GTGCCTGACA CCGTTACATA TGTGTGTAGC AACATGGAAT 
GTTGCTGTTA TCAAGAGATG GGTGGAAGTT TCCAGTCCAG AAGAAATTTC TCAGGCAATT 
AATATCCCAA GCCCAGTTGG CACTGCTCTG TGTATGGCTG CTTCTATAAG GAAAGATCAT 
GAAAAGGGTA GAGAGCTGGT TCAGATATTG CTTGCAGCTG GGGCAGATCC GACTGCGCAA 
GATGCTCAGC ACGGGCGAAC AGCGTTGCAT ACTGCTGCTA TGGCTAACAA TGTGGAACTT 
GTGAGAGTCA TTCTAGATGC TGGTGTGAAT GCGAACATCC GCAATGTTCA CAATACAATT 
CCTCTTCATA TGGCTTTGGC CAGAGGCGCA AACTCCTGTG TTAGCCTACT TCTGGAGTCT 
GGTTCAGACT GCAATATACA GGATGATGAA GGAGATAACG CATTTCATAT AGCAGCAGAT 
GCAGCGAAGA TGATACGTGA AAACCTTGAT TGGTTAATTG TCATGCTTAG AAGTCCTGAT 
GCTGCTGTGG ACGTCAGAAA CCACAGTGGC AAGACAGTAC GTGACTTCTT AGAAGCCCTT 
CCAAGAGAAT GGATATCAGA GGATCTGATG GAGGCACTAT TAAAAAGGGG AGTTCATCTA 
TCACCTACAA TCTATGAGGT AGGGGATTGG GTGAAATTCA AGAGAGGCAT AACAACACCC 
TTACATGGAT GGCAAGGTGC TAAGCCTAAG AGTGTTGGGT TTGTGCAAAC CATTCTCGAG 
AAGGAGGACA TGATTATCGC ATTTTGTTCT GGTGAAGCTC GTGTGTTGGC AAATGAAGTT 
GTCAAGTTGA TTCCATTAGA CAGGGGCCAG CATGTAAGAC TCAGAGCAGA TGTGAAAGAG 
CCAAGGTTTG GTTGGCGTGG TCAATCACGT GATAGTGTCG GTACTGTTTT ATGTGTTGAT 
GAGGATGGGA TCTTACGAGT TGGGTTTCCT GGAGCATCTC GAGGCTGGAA AGCTGATCCT 
GCTGAAATGG AACGTGTGGA AGAATTCAAA GTCGGGGATT GGGTCCGTAT CCGTCAGAAT 
CTGACTTCAG CCAAACATGG TTTTGGATCT GTTGTCCCCG GCAGTATGGG GATTGTTTAT 
TGTGTACGGC CAGATAGCAG TCTCCTTGTG GAGTTAAGTT ATCTTCCAAA TCCCTGGCAT 
TGTGAACCCG AGGAAGTTGA ACCTGTTGCT CCCTTTAGGA TTGGTGATCG GGTGTGTGTA 
AAGCGGTCAG TTGCCGAACC TCGATATGCT TGGGGTGGGG AGACCCATCA CAGTGTTGGA 
AAAATTAGTG AAATAGAGAA TGATGGTCTT CTTATTATTG AAATACCAAA CCGACCTATA 
CCGTGGCAGG CAGATCCTTC AGACATGGAA AAGATTGATG ATTTCAAGGT TGGTGACTGG 
GTCAGAGTGA AGGCGTCAGT GTCTTCCCCG AAATATGGAT GGGAAGACAT TACTCGAAAT 
AGTATTGGCG TTATGCACAG CTTAGATGAA GATGGTGATG TGGGTATAGC TTTCTGTTTC 
AGGAGTAAAC CTTTTTCATG TTCTGTGACA GATGTTGAAA AGGTTACGCC CTTTCATGTG 
GGACAAGAGA TTCATATGAC ACCATCAATC ACCCAACCAC GACTTGGATG GTCAAATGAG 
ACTCCAGCAA CTATTGGTAA AGTAATGAGA ATCGACATGG ACGGGACTCT GAGTGCACAG 
GTGACTGGTA GACAGACGTT GTGGAGGGTC TCACCTGGAG ATGCAGAGCT GCTTTCAGGT 
TTTGAAGTTG GTGATTGGGT GCGCTCAAAA CCGAGCCTGG GAAACAGACC AAGCTATGAT 
TGGTCTAATG TAGGAAGGGA GAGTATTGCT GTCGTTCATA GCATACAGGA AACAGGTTAC 
TTAGAATTGG CTTGTTGTTT CCGGAAAGGA AGATGGAGTA CTCATTACAC AGATTTAGAG 
AAGATTCCAG CTCTCAAAGT GGGCCAGTTT GTGCATTTCC AGAAGGGAAT AACAGAACCA 
AGATGGGGCT GGAGAGCAGC CAAGCCCGAT TCACGAGGCA TCATAACCAC AGTTCATGCT 
GATGGAGAGG TAAGGGTTGC TTTCTTTGGA TTGCCTGGGT TGTGGAGAGG AGATCCAGCA 
GACTTGGAAG TGGAGCCTAT GTTTGAGGTT GGAGAATGGG TAAGACTTAG AGAAGGTGTT 
TCTTGTTGGA AATCTGTTGG ACCAGGAAGT GTTGGTGTAG TGCATGGAGT AGGATATGAG 
GGAGACGAGT GGGATGGAAC CACTTCCGTT TCATTTTGTG GCGAGCAAGA AAGATGGGCG 
GGGCCCACTT CACACCTAGA GAAAGCAAAG AAGCTTGTGG TTGGACAAAA AACTCGGGTC 
AAACTGGCAG TGAAACAGCC TAGGTTTGGG TGGTCAGGTC ATAGCCATGG AAGTGTAGGA 
ACAATATCAG CCATTGATGC GGATGGTAAG CTACGGATAT ACACACCAGC CGGCTCTAAA 
ACGTGGATGC TTGACCCATC AGAAGTTGAA ACCATAGAAG AAGAAGAGCT CAAGATTGGG 
GACTGGGTTA GGGTAAAGGC GAGCATCACA ACACCCACTT ACCAGTGGGG AGAGGTCAAT 
CCTTCAAGCA CAGGGGTGGT TCACAGAATG GAGGATGGAG ATTTATGTGT ATCTTTCTGT 
TTCCTAGACA GGCTATGGCT ATGTAAAGCC GGGGAACTGG AACGCATAAG ACCATTTAGA 
ATTGGAGACC GGGTTAAGAT AAAGGATGGA CTGGTTACAC CTCGGTGGGG TTGGGGAATG 
GAGACCCATG CAAGTAAAGG ACATGTTGTG GGAGTGGATG CAAATGGGAA ATTGAGAATA 
AAATTCCTGT GGAGGGAAGG GCGGCCCTGG ATTGGTGATC CTGCGGATAT AGTTCTAGAT 
GAAACTTCTG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001190303.1
CDS	93..4967
Translation	MVGRVKVPCCSVCHTRYNEDERVPLLLQCGHGFCKDCLSKMFSTSSDTTLTCPRCRHVSVVGNSVQGLRKNYAMLALIHAASGGANFDCDYTDDEDDDDEEDGSDEDGARAARGFHASSSINSLCGPVIEVGAHPEMKLVRQIGEESSSGGFGGVEMWDATVAGGGGRCKHRVAVKKMTLTEDMDVEWMQGQLESLRRASMWCRNVCTFHGVVKMDGSLCLLMDRCFGSVQSEMQRNEGRLTLEQILRYGADVARGVAELHAAGVICMNIKPSNLLLDASGNAVVSDYGLAPILKKPTCQKTRPEFDSSKVTLYTDCVTLSPHYTAPEAWGPVKKLFWEDASGVSPESDAWSFGCTLVEMCTGSTPWDGLSREEIFQAVVKARKVPPQYERIVGVGIPRELWKMIGECLQFKPSKRPTFNAMLATFLRHLQEIPRSPSASPDNGIAKICEVNIVQAPRATNIGVFQDNPNNLHRVVLEGDFEGVRNILAKAAAGGGGSSVRSLLEAQNADGQSALHLACRRGSAELVEAILEYGEANVDIVDKDGDPPLVFALAAGSPQCVHVLIKKGANVRSRLREGSGPSVAHVCSYHGQPDCMRELLVAGADPNAVDDEGETVLHRAVAKKYTDCAIVILENGGSRSMTVSNAKCLTPLHMCVATWNVAVIKRWVEVSSPEEISQAINIPSPVGTALCMAASIRKDHEKGRELVQILLAAGADPTAQDAQHGRTALHTAAMANNVELVRVILDAGVNANIRNVHNTIPLHMALARGANSCVSLLLESGSDCNIQDDEGDNAFHIAADAAKMIRENLDWLIVMLRSPDAAVDVRNHSGKTVRDFLEALPREWISEDLMEALLKRGVHLSPTIYEVGDWVKFKRGITTPLHGWQGAKPKSVGFVQTILEKEDMIIAFCSGEARVLANEVVKLIPLDRGQHVRLRADVKEPRFGWRGQSRDSVGTVLCVDEDGILRVGFPGASRGWKADPAEMERVEEFKVGDWVRIRQNLTSAKHGFGSVVPGSMGIVYCVRPDSSLLVELSYLPNPWHCEPEEVEPVAPFRIGDRVCVKRSVAEPRYAWGGETHHSVGKISEIENDGLLIIEIPNRPIPWQADPSDMEKIDDFKVGDWVRVKASVSSPKYGWEDITRNSIGVMHSLDEDGDVGIAFCFRSKPFSCSVTDVEKVTPFHVGQEIHMTPSITQPRLGWSNETPATIGKVMRIDMDGTLSAQVTGRQTLWRVSPGDAELLSGFEVGDWVRSKPSLGNRPSYDWSNVGRESIAVVHSIQETGYLELACCFRKGRWSTHYTDLEKIPALKVGQFVHFQKGITEPRWGWRAAKPDSRGIITTVHADGEVRVAFFGLPGLWRGDPADLEVEPMFEVGEWVRLREGVSCWKSVGPGSVGVVHGVGYEGDEWDGTTSVSFCGEQERWAGPTSHLEKAKKLVVGQKTRVKLAVKQPRFGWSGHSHGSVGTISAIDADGKLRIYTPAGSKTWMLDPSEVETIEEEELKIGDWVRVKASITTPTYQWGEVNPSSTGVVHRMEDGDLCVSFCFLDRLWLCKAGELERIRPFRIGDRVKIKDGLVTPRWGWGMETHASKGHVVGVDANGKLRIKFLWREGRPWIGDPADIVLDETSG

Target ORF information:

RefSeq Version	NM_001203374.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana protein kinases;ubiquitin-protein ligase (KEG), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001203374.1

ORF Insert Sequence:

ATGGTTGGGA GAGTCAAGGT CCCTTGTTGT TCCGTGTGCC ACACGCGGTA TAATGAAGAT 
GAGAGAGTTC CTTTATTGCT TCAATGCGGC CATGGCTTCT GCAAAGATTG TCTTTCCAAG 
ATGTTCTCTA CTTCTTCCGA CACAACCCTC ACTTGTCCCC GGTGTCGCCA CGTGTCTGTT 
GTTGGGAACT CTGTTCAGGG TCTCAGGAAG AACTACGCTA TGCTTGCTCT CATTCACGCC 
GCATCCGGCG GCGCTAACTT CGATTGCGAT TACACGGATG ATGAGGATGA CGACGATGAA 
GAAGATGGTA GTGATGAAGA TGGAGCTCGT GCCGCTCGTG GATTTCATGC CTCAAGCTCG 
ATTAATAGCT TGTGTGGTCC AGTGATCGAG GTCGGAGCTC ACCCGGAGAT GAAGCTGGTG 
AGGCAGATTG GGGAAGAGAG TAGTAGTGGT GGTTTTGGAG GTGTGGAGAT GTGGGATGCA 
ACTGTTGCAG GCGGTGGTGG ACGGTGTAAG CACCGTGTGG CGGTGAAAAA GATGACTTTG 
ACGGAGGACA TGGATGTTGA GTGGATGCAG GGACAGCTTG AGAGCTTGCG GAGAGCATCC 
ATGTGGTGTA GGAATGTTTG TACTTTCCAT GGGGTTGTGA AGATGGATGG ATCTCTTTGT 
CTTTTGATGG ACAGGTGCTT TGGGTCTGTT CAGTCTGAGA TGCAACGCAA TGAAGGCAGA 
CTTACTTTGG AGCAGATTCT AAGATATGGG GCTGATGTTG CTCGAGGGGT TGCAGAACTC 
CATGCAGCAG GTGTTATCTG TATGAATATA AAACCTTCCA ATCTTCTTTT GGATGCAAGC 
GGCAATGCTG TGGTTTCTGA TTATGGGCTC GCTCCCATCC TAAAGAAGCC AACATGCCAG 
AAAACTCGAC CAGAGTTTGA TTCCTCAAAA GTCACTCTAT ACACGGACTG TGTAACACTC 
AGTCCACACT ACACTGCCCC AGAGGCTTGG GGACCGGTGA AAAAACTTTT TTGGGAGGAT 
GCAAGTGGTG TATCTCCAGA ATCAGATGCA TGGAGTTTTG GCTGTACGTT AGTAGAGATG 
TGCACTGGAT CCACTCCGTG GGATGGTCTT AGTAGAGAAG AAATTTTTCA AGCTGTTGTG 
AAAGCCCGAA AAGTACCACC ACAGTATGAA CGCATAGTGG GTGTGGGAAT TCCTCGGGAA 
TTATGGAAAA TGATTGGTGA ATGTCTTCAG TTTAAACCGT CGAAAAGACC AACCTTTAAT 
GCAATGCTTG CTACATTTCT TCGTCATCTA CAAGAGATAC CACGTAGCCC TTCAGCAAGC 
CCTGATAATG GGATTGCCAA AATATGTGAA GTAAACATTG TACAAGCACC TCGCGCGACT 
AACATAGGGG TCTTTCAGGA TAACCCTAAC AACCTACATC GGGTTGTTTT GGAGGGAGAC 
TTTGAGGGTG TAAGAAATAT ACTTGCAAAG GCTGCAGCTG GAGGCGGTGG AAGCTCTGTG 
CGATCTCTAT TAGAGGCGCA AAACGCTGAT GGTCAATCGG CTCTTCACTT AGCTTGCAGA 
CGTGGCAGTG CTGAACTTGT GGAGGCTATA CTGGAGTATG GGGAGGCTAA TGTAGATATC 
GTGGACAAAG ATGGGGATCC TCCTCTTGTC TTTGCTTTAG CAGCTGGATC TCCACAATGT 
GTTCACGTTC TCATTAAAAA GGGGGCTAAT GTGAGGTCCA GACTGCGGGA AGGATCTGGC 
CCATCTGTAG CTCACGTGTG TTCGTACCAT GGCCAACCTG ATTGTATGCG TGAATTGCTG 
GTAGCTGGAG CCGATCCAAA TGCAGTGGAT GATGAAGGTG AAACTGTGCT TCATCGAGCT 
GTTGCCAAGA AATACACAGA TTGTGCCATT GTTATATTGG AAAATGGGGG ATCTAGATCA 
ATGACTGTTT CAAATGCGAA GTGCCTGACA CCGTTACATA TGTGTGTAGC AACATGGAAT 
GTTGCTGTTA TCAAGAGATG GGTGGAAGTT TCCAGTCCAG AAGAAATTTC TCAGGCAATT 
AATATCCCAA GCCCAGTTGG CACTGCTCTG TGTATGGCTG CTTCTATAAG GAAAGATCAT 
GAAAAGGGTA GAGAGCTGGT TCAGATATTG CTTGCAGCTG GGGCAGATCC GACTGCGCAA 
GATGCTCAGC ACGGGCGAAC AGCGTTGCAT ACTGCTGCTA TGGCTAACAA TGTGGAACTT 
GTGAGAGTCA TTCTAGATGC TGGTGTGAAT GCGAACATCC GCAATGTTCA CAATACAATT 
CCTCTTCATA TGGCTTTGGC CAGAGGCGCA AACTCCTGTG TTAGCCTACT TCTGGAGTCT 
GGTTCAGACT GCAATATACA GGATGATGAA GGAGATAACG CATTTCATAT AGCAGCAGAT 
GCAGCGAAGA TGATACGTGA AAACCTTGAT TGGTTAATTG TCATGCTTAG AAGTCCTGAT 
GCTGCTGTGG ACGTCAGAAA CCACAGTGGC AAGACAGTAC GTGACTTCTT AGAAGCCCTT 
CCAAGAGAAT GGATATCAGA GGATCTGATG GAGGCACTAT TAAAAAGGGG AGTTCATCTA 
TCACCTACAA TCTATGAGGT AGGGGATTGG GTGAAATTCA AGAGAGGCAT AACAACACCC 
TTACATGGAT GGCAAGGTGC TAAGCCTAAG AGTGTTGGGT TTGTGCAAAC CATTCTCGAG 
AAGGAGGACA TGATTATCGC ATTTTGTTCT GGTGAAGCTC GTGTGTTGGC AAATGAAGTT 
GTCAAGTTGA TTCCATTAGA CAGGGGCCAG CATGTAAGAC TCAGAGCAGA TGTGAAAGAG 
CCAAGGTTTG GTTGGCGTGG TCAATCACGT GATAGTGTCG GTACTGTTTT ATGTGTTGAT 
GAGGATGGGA TCTTACGAGT TGGGTTTCCT GGAGCATCTC GAGGCTGGAA AGCTGATCCT 
GCTGAAATGG AACGTGTGGA AGAATTCAAA GTCGGGGATT GGGTCCGTAT CCGTCAGAAT 
CTGACTTCAG CCAAACATGG TTTTGGATCT GTTGTCCCCG GCAGTATGGG GATTGTTTAT 
TGTGTACGGC CAGATAGCAG TCTCCTTGTG GAGTTAAGTT ATCTTCCAAA TCCCTGGCAT 
TGTGAACCCG AGGAAGTTGA ACCTGTTGCT CCCTTTAGGA TTGGTGATCG GGTGTGTGTA 
AAGCGGTCAG TTGCCGAACC TCGATATGCT TGGGGTGGGG AGACCCATCA CAGTGTTGGA 
AAAATTAGTG AAATAGAGAA TGATGGTCTT CTTATTATTG AAATACCAAA CCGACCTATA 
CCGTGGCAGG CAGATCCTTC AGACATGGAA AAGATTGATG ATTTCAAGGT TGGTGACTGG 
GTCAGAGTGA AGGCGTCAGT GTCTTCCCCG AAATATGGAT GGGAAGACAT TACTCGAAAT 
AGTATTGGCG TTATGCACAG CTTAGATGAA GATGGTGATG TGGGTATAGC TTTCTGTTTC 
AGGAGTAAAC CTTTTTCATG TTCTGTGACA GATGTTGAAA AGGTTACGCC CTTTCATGTG 
GGACAAGAGA TTCATATGAC ACCATCAATC ACCCAACCAC GACTTGGATG GTCAAATGAG 
ACTCCAGCAA CTATTGGTAA AGTAATGAGA ATCGACATGG ACGGGACTCT GAGTGCACAG 
GTGACTGGTA GACAGACGTT GTGGAGGGTC TCACCTGGAG ATGCAGAGCT GCTTTCAGGT 
TTTGAAGTTG GTGATTGGGT GCGCTCAAAA CCGAGCCTGG GAAACAGACC AAGCTATGAT 
TGGTCTAATG TAGGAAGGGA GAGTATTGCT GTCGTTCATA GCATACAGGA AACAGGTTAC 
TTAGAATTGG CTTGTTGTTT CCGGAAAGGA AGATGGAGTA CTCATTACAC AGATTTAGAG 
AAGATTCCAG CTCTCAAAGT GGGCCAGTTT GTGCATTTCC AGAAGGGAAT AACAGAACCA 
AGATGGGGCT GGAGAGCAGC CAAGCCCGAT TCACGAGGCA TCATAACCAC AGTTCATGCT 
GATGGAGAGG TAAGGGTTGC TTTCTTTGGA TTGCCTGGGT TGTGGAGAGG AGATCCAGCA 
GACTTGGAAG TGGAGCCTAT GTTTGAGGTT GGAGAATGGG TAAGACTTAG AGAAGGTGTT 
TCTTGTTGGA AATCTGTTGG ACCAGGAAGT GTTGGTGTAG TGCATGGAGT AGGATATGAG 
GGAGACGAGT GGGATGGAAC CACTTCCGTT TCATTTTGTG GCGAGCAAGA AAGATGGGCG 
GGGCCCACTT CACACCTAGA GAAAGCAAAG AAGCTTGTGG TTGGACAAAA AACTCGGGTC 
AAACTGGCAG TGAAACAGCC TAGGTTTGGG TGGTCAGGTC ATAGCCATGG AAGTGTAGGA 
ACAATATCAG CCATTGATGC GGATGGTAAG CTACGGATAT ACACACCAGC CGGCTCTAAA 
ACGTGGATGC TTGACCCATC AGAAGTTGAA ACCATAGAAG AAGAAGAGCT CAAGATTGGG 
GACTGGGTTA GGGTAAAGGC GAGCATCACA ACACCCACTT ACCAGTGGGG AGAGGTCAAT 
CCTTCAAGCA CAGGGGTGGT TCACAGAATG GAGGATGGAG ATTTATGTGT ATCTTTCTGT 
TTCCTAGACA GGCTATGGCT ATGTAAAGCC GGGGAACTGG AACGCATAAG ACCATTTAGA 
ATTGGAGACC GGGTTAAGAT AAAGGATGGA CTGGTTACAC CTCGGTGGGG TTGGGGAATG 
GAGACCCATG CAAGTAAAGG ACATGTTGTG GGAGTGGATG CAAATGGGAA ATTGAGAATA 
AAATTCCTGT GGAGGGAAGG GCGGCCCTGG ATTGGTGATC CTGCGGATAT AGTTCTAGAT 
GAAACTTCTG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KEG ( NM_121356.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_196857.2 Arabidopsis thaliana protein kinases;ubiquitin-protein ligase (KEG), mRNA.

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CloneID	OAb18298
Clone ID Related Accession (Same CDS sequence)	NM_121356.3
Accession Version	NM_121356.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	protein kinases;ubiquitin-protein ligase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_121356.2.

ATGGTTGGGA GAGTCAAGGT CCCTTGTTGT TCCGTGTGCC ACACGCGGTA TAATGAAGAT 
GAGAGAGTTC CTTTATTGCT TCAATGCGGC CATGGCTTCT GCAAAGATTG TCTTTCCAAG 
ATGTTCTCTA CTTCTTCCGA CACAACCCTC ACTTGTCCCC GGTGTCGCCA CGTGTCTGTT 
GTTGGGAACT CTGTTCAGGG TCTCAGGAAG AACTACGCTA TGCTTGCTCT CATTCACGCC 
GCATCCGGCG GCGCTAACTT CGATTGCGAT TACACGGATG ATGAGGATGA CGACGATGAA 
GAAGATGGTA GTGATGAAGA TGGAGCTCGT GCCGCTCGTG GATTTCATGC CTCAAGCTCG 
ATTAATAGCT TGTGTGGTCC AGTGATCGAG GTCGGAGCTC ACCCGGAGAT GAAGCTGGTG 
AGGCAGATTG GGGAAGAGAG TAGTAGTGGT GGTTTTGGAG GTGTGGAGAT GTGGGATGCA 
ACTGTTGCAG GCGGTGGTGG ACGGTGTAAG CACCGTGTGG CGGTGAAAAA GATGACTTTG 
ACGGAGGACA TGGATGTTGA GTGGATGCAG GGACAGCTTG AGAGCTTGCG GAGAGCATCC 
ATGTGGTGTA GGAATGTTTG TACTTTCCAT GGGGTTGTGA AGATGGATGG ATCTCTTTGT 
CTTTTGATGG ACAGGTGCTT TGGGTCTGTT CAGTCTGAGA TGCAACGCAA TGAAGGCAGA 
CTTACTTTGG AGCAGATTCT AAGATATGGG GCTGATGTTG CTCGAGGGGT TGCAGAACTC 
CATGCAGCAG GTGTTATCTG TATGAATATA AAACCTTCCA ATCTTCTTTT GGATGCAAGC 
GGCAATGCTG TGGTTTCTGA TTATGGGCTC GCTCCCATCC TAAAGAAGCC AACATGCCAG 
AAAACTCGAC CAGAGTTTGA TTCCTCAAAA GTCACTCTAT ACACGGACTG TGTAACACTC 
AGTCCACACT ACACTGCCCC AGAGGCTTGG GGACCGGTGA AAAAACTTTT TTGGGAGGAT 
GCAAGTGGTG TATCTCCAGA ATCAGATGCA TGGAGTTTTG GCTGTACGTT AGTAGAGATG 
TGCACTGGAT CCACTCCGTG GGATGGTCTT AGTAGAGAAG AAATTTTTCA AGCTGTTGTG 
AAAGCCCGAA AAGTACCACC ACAGTATGAA CGCATAGTGG GTGTGGGAAT TCCTCGGGAA 
TTATGGAAAA TGATTGGTGA ATGTCTTCAG TTTAAACCGT CGAAAAGACC AACCTTTAAT 
GCAATGCTTG CTACATTTCT TCGTCATCTA CAAGAGATAC CACGTAGCCC TTCAGCAAGC 
CCTGATAATG GGATTGCCAA AATATGTGAA GTAAACATTG TACAAGCACC TCGCGCGACT 
AACATAGGGG TCTTTCAGGA TAACCCTAAC AACCTACATC GGGTTGTTTT GGAGGGAGAC 
TTTGAGGGTG TAAGAAATAT ACTTGCAAAG GCTGCAGCTG GAGGCGGTGG AAGCTCTGTG 
CGATCTCTAT TAGAGGCGCA AAACGCTGAT GGTCAATCGG CTCTTCACTT AGCTTGCAGA 
CGTGGCAGTG CTGAACTTGT GGAGGCTATA CTGGAGTATG GGGAGGCTAA TGTAGATATC 
GTGGACAAAG ATGGGGATCC TCCTCTTGTC TTTGCTTTAG CAGCTGGATC TCCACAATGT 
GTTCACGTTC TCATTAAAAA GGGGGCTAAT GTGAGGTCCA GACTGCGGGA AGGATCTGGC 
CCATCTGTAG CTCACGTGTG TTCGTACCAT GGCCAACCTG ATTGTATGCG TGAATTGCTG 
GTAGCTGGAG CCGATCCAAA TGCAGTGGAT GATGAAGGTG AAACTGTGCT TCATCGAGCT 
GTTGCCAAGA AATACACAGA TTGTGCCATT GTTATATTGG AAAATGGGGG ATCTAGATCA 
ATGACTGTTT CAAATGCGAA GTGCCTGACA CCGTTACATA TGTGTGTAGC AACATGGAAT 
GTTGCTGTTA TCAAGAGATG GGTGGAAGTT TCCAGTCCAG AAGAAATTTC TCAGGCAATT 
AATATCCCAA GCCCAGTTGG CACTGCTCTG TGTATGGCTG CTTCTATAAG GAAAGATCAT 
GAAAAGGAAG GTAGAGAGCT GGTTCAGATA TTGCTTGCAG CTGGGGCAGA TCCGACTGCG 
CAAGATGCTC AGCACGGGCG AACAGCGTTG CATACTGCTG CTATGGCTAA CAATGTGGAA 
CTTGTGAGAG TCATTCTAGA TGCTGGTGTG AATGCGAACA TCCGCAATGT TCACAATACA 
ATTCCTCTTC ATATGGCTTT GGCCAGAGGC GCAAACTCCT GTGTTAGCCT ACTTCTGGAG 
TCTGGTTCAG ACTGCAATAT ACAGGATGAT GAAGGAGATA ACGCATTTCA TATAGCAGCA 
GATGCAGCGA AGATGATACG TGAAAACCTT GATTGGTTAA TTGTCATGCT TAGAAGTCCT 
GATGCTGCTG TGGACGTCAG AAACCACAGT GGCAAGACAG TACGTGACTT CTTAGAAGCC 
CTTCCAAGAG AATGGATATC AGAGGATCTG ATGGAGGCAC TATTAAAAAG GGGAGTTCAT 
CTATCACCTA CAATCTATGA GGTAGGGGAT TGGGTGAAAT TCAAGAGAGG CATAACAACA 
CCCTTACATG GATGGCAAGG TGCTAAGCCT AAGAGTGTTG GGTTTGTGCA AACCATTCTC 
GAGAAGGAGG ACATGATTAT CGCATTTTGT TCTGGTGAAG CTCGTGTGTT GGCAAATGAA 
GTTGTCAAGT TGATTCCATT AGACAGGGGC CAGCATGTAA GACTCAGAGC AGATGTGAAA 
GAGCCAAGGT TTGGTTGGCG TGGTCAATCA CGTGATAGTG TCGGTACTGT TTTATGTGTT 
GATGAGGATG GGATCTTACG AGTTGGGTTT CCTGGAGCAT CTCGAGGCTG GAAAGCTGAT 
CCTGCTGAAA TGGAACGTGT GGAAGAATTC AAAGTCGGGG ATTGGGTCCG TATCCGTCAG 
AATCTGACTT CAGCCAAACA TGGTTTTGGA TCTGTTGTCC CCGGCAGTAT GGGGATTGTT 
TATTGTGTAC GGCCAGATAG CAGTCTCCTT GTGGAGTTAA GTTATCTTCC AAATCCCTGG 
CATTGTGAAC CCGAGGAAGT TGAACCTGTT GCTCCCTTTA GGATTGGTGA TCGGGTGTGT 
GTAAAGCGGT CAGTTGCCGA ACCTCGATAT GCTTGGGGTG GGGAGACCCA TCACAGTGTT 
GGAAAAATTA GTGAAATAGA GAATGATGGT CTTCTTATTA TTGAAATACC AAACCGACCT 
ATACCGTGGC AGGCAGATCC TTCAGACATG GAAAAGATTG ATGATTTCAA GGTTGGTGAC 
TGGGTCAGAG TGAAGGCGTC AGTGTCTTCC CCGAAATATG GATGGGAAGA CATTACTCGA 
AATAGTATTG GCGTTATGCA CAGCTTAGAT GAAGATGGTG ATGTGGGTAT AGCTTTCTGT 
TTCAGGAGTA AACCTTTTTC ATGTTCTGTG ACAGATGTTG AAAAGGTTAC GCCCTTTCAT 
GTGGGACAAG AGATTCATAT GACACCATCA ATCACCCAAC CACGACTTGG ATGGTCAAAT 
GAGACTCCAG CAACTATTGG TAAAGTAATG AGAATCGACA TGGACGGGAC TCTGAGTGCA 
CAGGTGACTG GTAGACAGAC GTTGTGGAGG GTCTCACCTG GAGATGCAGA GCTGCTTTCA 
GGTTTTGAAG TTGGTGATTG GGTGCGCTCA AAACCGAGCC TGGGAAACAG ACCAAGCTAT 
GATTGGTCTA ATGTAGGAAG GGAGAGTATT GCTGTCGTTC ATAGCATACA GGAAACAGGT 
TACTTAGAAT TGGCTTGTTG TTTCCGGAAA GGAAGATGGA GTACTCATTA CACAGATTTA 
GAGAAGATTC CAGCTCTCAA AGTGGGCCAG TTTGTGCATT TCCAGAAGGG AATAACAGAA 
CCAAGATGGG GCTGGAGAGC AGCCAAGCCC GATTCACGAG GCATCATAAC CACAGTTCAT 
GCTGATGGAG AGGTAAGGGT TGCTTTCTTT GGATTGCCTG GGTTGTGGAG AGGAGATCCA 
GCAGACTTGG AAGTGGAGCC TATGTTTGAG GTTGGAGAAT GGGTAAGACT TAGAGAAGGT 
GTTTCTTGTT GGAAATCTGT TGGACCAGGA AGTGTTGGTG TAGTGCATGG AGTAGGATAT 
GAGGGAGACG AGTGGGATGG AACCACTTCC GTTTCATTTT GTGGCGAGCA AGAAAGATGG 
GCGGGGCCCA CTTCACACCT AGAGAAAGCA AAGAAGCTTG TGGTTGGACA AAAAACTCGG 
GTCAAACTGG CAGTGAAACA GCCTAGGTTT GGGTGGTCAG GTCATAGCCA TGGAAGTGTA 
GGAACAATAT CAGCCATTGA TGCGGATGGT AAGCTACGGA TATACACACC AGCCGGCTCT 
AAAACGTGGA TGCTTGACCC ATCAGAAGTT GAAACCATAG AAGAAGAAGA GCTCAAGATT 
GGGGACTGGG TTAGGGTAAA GGCGAGCATC ACAACACCCA CTTACCAGTG GGGAGAGGTC 
AATCCTTCAA GCACAGGGGT GGTTCACAGA ATGGAGGATG GAGATTTATG TGTATCTTTC 
TGTTTCCTAG ACAGGCTATG GCTATGTAAA GCCGGGGAAC TGGAACGCAT AAGACCATTT 
AGAATTGGAG ACCGGGTTAA GATAAAGGAT GGACTGGTTA CACCTCGGTG GGGTTGGGGA 
ATGGAGACCC ATGCAAGTAA AGGACATGTT GTGGGAGTGG ATGCAAATGG GAAATTGAGA 
ATAAAATTCC TGTGGAGGGA AGGGCGGCCC TGGATTGGTG ATCCTGCGGA TATAGTTCTA 
GATGAAACTT CTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_196857.2
CDS	417..5294
Translation	MVGRVKVPCCSVCHTRYNEDERVPLLLQCGHGFCKDCLSKMFSTSSDTTLTCPRCRHVSVVGNSVQGLRKNYAMLALIHAASGGANFDCDYTDDEDDDDEEDGSDEDGARAARGFHASSSINSLCGPVIEVGAHPEMKLVRQIGEESSSGGFGGVEMWDATVAGGGGRCKHRVAVKKMTLTEDMDVEWMQGQLESLRRASMWCRNVCTFHGVVKMDGSLCLLMDRCFGSVQSEMQRNEGRLTLEQILRYGADVARGVAELHAAGVICMNIKPSNLLLDASGNAVVSDYGLAPILKKPTCQKTRPEFDSSKVTLYTDCVTLSPHYTAPEAWGPVKKLFWEDASGVSPESDAWSFGCTLVEMCTGSTPWDGLSREEIFQAVVKARKVPPQYERIVGVGIPRELWKMIGECLQFKPSKRPTFNAMLATFLRHLQEIPRSPSASPDNGIAKICEVNIVQAPRATNIGVFQDNPNNLHRVVLEGDFEGVRNILAKAAAGGGGSSVRSLLEAQNADGQSALHLACRRGSAELVEAILEYGEANVDIVDKDGDPPLVFALAAGSPQCVHVLIKKGANVRSRLREGSGPSVAHVCSYHGQPDCMRELLVAGADPNAVDDEGETVLHRAVAKKYTDCAIVILENGGSRSMTVSNAKCLTPLHMCVATWNVAVIKRWVEVSSPEEISQAINIPSPVGTALCMAASIRKDHEKEGRELVQILLAAGADPTAQDAQHGRTALHTAAMANNVELVRVILDAGVNANIRNVHNTIPLHMALARGANSCVSLLLESGSDCNIQDDEGDNAFHIAADAAKMIRENLDWLIVMLRSPDAAVDVRNHSGKTVRDFLEALPREWISEDLMEALLKRGVHLSPTIYEVGDWVKFKRGITTPLHGWQGAKPKSVGFVQTILEKEDMIIAFCSGEARVLANEVVKLIPLDRGQHVRLRADVKEPRFGWRGQSRDSVGTVLCVDEDGILRVGFPGASRGWKADPAEMERVEEFKVGDWVRIRQNLTSAKHGFGSVVPGSMGIVYCVRPDSSLLVELSYLPNPWHCEPEEVEPVAPFRIGDRVCVKRSVAEPRYAWGGETHHSVGKISEIENDGLLIIEIPNRPIPWQADPSDMEKIDDFKVGDWVRVKASVSSPKYGWEDITRNSIGVMHSLDEDGDVGIAFCFRSKPFSCSVTDVEKVTPFHVGQEIHMTPSITQPRLGWSNETPATIGKVMRIDMDGTLSAQVTGRQTLWRVSPGDAELLSGFEVGDWVRSKPSLGNRPSYDWSNVGRESIAVVHSIQETGYLELACCFRKGRWSTHYTDLEKIPALKVGQFVHFQKGITEPRWGWRAAKPDSRGIITTVHADGEVRVAFFGLPGLWRGDPADLEVEPMFEVGEWVRLREGVSCWKSVGPGSVGVVHGVGYEGDEWDGTTSVSFCGEQERWAGPTSHLEKAKKLVVGQKTRVKLAVKQPRFGWSGHSHGSVGTISAIDADGKLRIYTPAGSKTWMLDPSEVETIEEEELKIGDWVRVKASITTPTYQWGEVNPSSTGVVHRMEDGDLCVSFCFLDRLWLCKAGELERIRPFRIGDRVKIKDGLVTPRWGWGMETHASKGHVVGVDANGKLRIKFLWREGRPWIGDPADIVLDETSG

Target ORF information:

RefSeq Version	NM_121356.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana protein kinases;ubiquitin-protein ligase (KEG), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_121356.3

ORF Insert Sequence:

ATGGTTGGGA GAGTCAAGGT CCCTTGTTGT TCCGTGTGCC ACACGCGGTA TAATGAAGAT 
GAGAGAGTTC CTTTATTGCT TCAATGCGGC CATGGCTTCT GCAAAGATTG TCTTTCCAAG 
ATGTTCTCTA CTTCTTCCGA CACAACCCTC ACTTGTCCCC GGTGTCGCCA CGTGTCTGTT 
GTTGGGAACT CTGTTCAGGG TCTCAGGAAG AACTACGCTA TGCTTGCTCT CATTCACGCC 
GCATCCGGCG GCGCTAACTT CGATTGCGAT TACACGGATG ATGAGGATGA CGACGATGAA 
GAAGATGGTA GTGATGAAGA TGGAGCTCGT GCCGCTCGTG GATTTCATGC CTCAAGCTCG 
ATTAATAGCT TGTGTGGTCC AGTGATCGAG GTCGGAGCTC ACCCGGAGAT GAAGCTGGTG 
AGGCAGATTG GGGAAGAGAG TAGTAGTGGT GGTTTTGGAG GTGTGGAGAT GTGGGATGCA 
ACTGTTGCAG GCGGTGGTGG ACGGTGTAAG CACCGTGTGG CGGTGAAAAA GATGACTTTG 
ACGGAGGACA TGGATGTTGA GTGGATGCAG GGACAGCTTG AGAGCTTGCG GAGAGCATCC 
ATGTGGTGTA GGAATGTTTG TACTTTCCAT GGGGTTGTGA AGATGGATGG ATCTCTTTGT 
CTTTTGATGG ACAGGTGCTT TGGGTCTGTT CAGTCTGAGA TGCAACGCAA TGAAGGCAGA 
CTTACTTTGG AGCAGATTCT AAGATATGGG GCTGATGTTG CTCGAGGGGT TGCAGAACTC 
CATGCAGCAG GTGTTATCTG TATGAATATA AAACCTTCCA ATCTTCTTTT GGATGCAAGC 
GGCAATGCTG TGGTTTCTGA TTATGGGCTC GCTCCCATCC TAAAGAAGCC AACATGCCAG 
AAAACTCGAC CAGAGTTTGA TTCCTCAAAA GTCACTCTAT ACACGGACTG TGTAACACTC 
AGTCCACACT ACACTGCCCC AGAGGCTTGG GGACCGGTGA AAAAACTTTT TTGGGAGGAT 
GCAAGTGGTG TATCTCCAGA ATCAGATGCA TGGAGTTTTG GCTGTACGTT AGTAGAGATG 
TGCACTGGAT CCACTCCGTG GGATGGTCTT AGTAGAGAAG AAATTTTTCA AGCTGTTGTG 
AAAGCCCGAA AAGTACCACC ACAGTATGAA CGCATAGTGG GTGTGGGAAT TCCTCGGGAA 
TTATGGAAAA TGATTGGTGA ATGTCTTCAG TTTAAACCGT CGAAAAGACC AACCTTTAAT 
GCAATGCTTG CTACATTTCT TCGTCATCTA CAAGAGATAC CACGTAGCCC TTCAGCAAGC 
CCTGATAATG GGATTGCCAA AATATGTGAA GTAAACATTG TACAAGCACC TCGCGCGACT 
AACATAGGGG TCTTTCAGGA TAACCCTAAC AACCTACATC GGGTTGTTTT GGAGGGAGAC 
TTTGAGGGTG TAAGAAATAT ACTTGCAAAG GCTGCAGCTG GAGGCGGTGG AAGCTCTGTG 
CGATCTCTAT TAGAGGCGCA AAACGCTGAT GGTCAATCGG CTCTTCACTT AGCTTGCAGA 
CGTGGCAGTG CTGAACTTGT GGAGGCTATA CTGGAGTATG GGGAGGCTAA TGTAGATATC 
GTGGACAAAG ATGGGGATCC TCCTCTTGTC TTTGCTTTAG CAGCTGGATC TCCACAATGT 
GTTCACGTTC TCATTAAAAA GGGGGCTAAT GTGAGGTCCA GACTGCGGGA AGGATCTGGC 
CCATCTGTAG CTCACGTGTG TTCGTACCAT GGCCAACCTG ATTGTATGCG TGAATTGCTG 
GTAGCTGGAG CCGATCCAAA TGCAGTGGAT GATGAAGGTG AAACTGTGCT TCATCGAGCT 
GTTGCCAAGA AATACACAGA TTGTGCCATT GTTATATTGG AAAATGGGGG ATCTAGATCA 
ATGACTGTTT CAAATGCGAA GTGCCTGACA CCGTTACATA TGTGTGTAGC AACATGGAAT 
GTTGCTGTTA TCAAGAGATG GGTGGAAGTT TCCAGTCCAG AAGAAATTTC TCAGGCAATT 
AATATCCCAA GCCCAGTTGG CACTGCTCTG TGTATGGCTG CTTCTATAAG GAAAGATCAT 
GAAAAGGAAG GTAGAGAGCT GGTTCAGATA TTGCTTGCAG CTGGGGCAGA TCCGACTGCG 
CAAGATGCTC AGCACGGGCG AACAGCGTTG CATACTGCTG CTATGGCTAA CAATGTGGAA 
CTTGTGAGAG TCATTCTAGA TGCTGGTGTG AATGCGAACA TCCGCAATGT TCACAATACA 
ATTCCTCTTC ATATGGCTTT GGCCAGAGGC GCAAACTCCT GTGTTAGCCT ACTTCTGGAG 
TCTGGTTCAG ACTGCAATAT ACAGGATGAT GAAGGAGATA ACGCATTTCA TATAGCAGCA 
GATGCAGCGA AGATGATACG TGAAAACCTT GATTGGTTAA TTGTCATGCT TAGAAGTCCT 
GATGCTGCTG TGGACGTCAG AAACCACAGT GGCAAGACAG TACGTGACTT CTTAGAAGCC 
CTTCCAAGAG AATGGATATC AGAGGATCTG ATGGAGGCAC TATTAAAAAG GGGAGTTCAT 
CTATCACCTA CAATCTATGA GGTAGGGGAT TGGGTGAAAT TCAAGAGAGG CATAACAACA 
CCCTTACATG GATGGCAAGG TGCTAAGCCT AAGAGTGTTG GGTTTGTGCA AACCATTCTC 
GAGAAGGAGG ACATGATTAT CGCATTTTGT TCTGGTGAAG CTCGTGTGTT GGCAAATGAA 
GTTGTCAAGT TGATTCCATT AGACAGGGGC CAGCATGTAA GACTCAGAGC AGATGTGAAA 
GAGCCAAGGT TTGGTTGGCG TGGTCAATCA CGTGATAGTG TCGGTACTGT TTTATGTGTT 
GATGAGGATG GGATCTTACG AGTTGGGTTT CCTGGAGCAT CTCGAGGCTG GAAAGCTGAT 
CCTGCTGAAA TGGAACGTGT GGAAGAATTC AAAGTCGGGG ATTGGGTCCG TATCCGTCAG 
AATCTGACTT CAGCCAAACA TGGTTTTGGA TCTGTTGTCC CCGGCAGTAT GGGGATTGTT 
TATTGTGTAC GGCCAGATAG CAGTCTCCTT GTGGAGTTAA GTTATCTTCC AAATCCCTGG 
CATTGTGAAC CCGAGGAAGT TGAACCTGTT GCTCCCTTTA GGATTGGTGA TCGGGTGTGT 
GTAAAGCGGT CAGTTGCCGA ACCTCGATAT GCTTGGGGTG GGGAGACCCA TCACAGTGTT 
GGAAAAATTA GTGAAATAGA GAATGATGGT CTTCTTATTA TTGAAATACC AAACCGACCT 
ATACCGTGGC AGGCAGATCC TTCAGACATG GAAAAGATTG ATGATTTCAA GGTTGGTGAC 
TGGGTCAGAG TGAAGGCGTC AGTGTCTTCC CCGAAATATG GATGGGAAGA CATTACTCGA 
AATAGTATTG GCGTTATGCA CAGCTTAGAT GAAGATGGTG ATGTGGGTAT AGCTTTCTGT 
TTCAGGAGTA AACCTTTTTC ATGTTCTGTG ACAGATGTTG AAAAGGTTAC GCCCTTTCAT 
GTGGGACAAG AGATTCATAT GACACCATCA ATCACCCAAC CACGACTTGG ATGGTCAAAT 
GAGACTCCAG CAACTATTGG TAAAGTAATG AGAATCGACA TGGACGGGAC TCTGAGTGCA 
CAGGTGACTG GTAGACAGAC GTTGTGGAGG GTCTCACCTG GAGATGCAGA GCTGCTTTCA 
GGTTTTGAAG TTGGTGATTG GGTGCGCTCA AAACCGAGCC TGGGAAACAG ACCAAGCTAT 
GATTGGTCTA ATGTAGGAAG GGAGAGTATT GCTGTCGTTC ATAGCATACA GGAAACAGGT 
TACTTAGAAT TGGCTTGTTG TTTCCGGAAA GGAAGATGGA GTACTCATTA CACAGATTTA 
GAGAAGATTC CAGCTCTCAA AGTGGGCCAG TTTGTGCATT TCCAGAAGGG AATAACAGAA 
CCAAGATGGG GCTGGAGAGC AGCCAAGCCC GATTCACGAG GCATCATAAC CACAGTTCAT 
GCTGATGGAG AGGTAAGGGT TGCTTTCTTT GGATTGCCTG GGTTGTGGAG AGGAGATCCA 
GCAGACTTGG AAGTGGAGCC TATGTTTGAG GTTGGAGAAT GGGTAAGACT TAGAGAAGGT 
GTTTCTTGTT GGAAATCTGT TGGACCAGGA AGTGTTGGTG TAGTGCATGG AGTAGGATAT 
GAGGGAGACG AGTGGGATGG AACCACTTCC GTTTCATTTT GTGGCGAGCA AGAAAGATGG 
GCGGGGCCCA CTTCACACCT AGAGAAAGCA AAGAAGCTTG TGGTTGGACA AAAAACTCGG 
GTCAAACTGG CAGTGAAACA GCCTAGGTTT GGGTGGTCAG GTCATAGCCA TGGAAGTGTA 
GGAACAATAT CAGCCATTGA TGCGGATGGT AAGCTACGGA TATACACACC AGCCGGCTCT 
AAAACGTGGA TGCTTGACCC ATCAGAAGTT GAAACCATAG AAGAAGAAGA GCTCAAGATT 
GGGGACTGGG TTAGGGTAAA GGCGAGCATC ACAACACCCA CTTACCAGTG GGGAGAGGTC 
AATCCTTCAA GCACAGGGGT GGTTCACAGA ATGGAGGATG GAGATTTATG TGTATCTTTC 
TGTTTCCTAG ACAGGCTATG GCTATGTAAA GCCGGGGAAC TGGAACGCAT AAGACCATTT 
AGAATTGGAG ACCGGGTTAA GATAAAGGAT GGACTGGTTA CACCTCGGTG GGGTTGGGGA 
ATGGAGACCC ATGCAAGTAA AGGACATGTT GTGGGAGTGG ATGCAAATGG GAAATTGAGA 
ATAAAATTCC TGTGGAGGGA AGGGCGGCCC TGGATTGGTG ATCCTGCGGA TATAGTTCTA 
GATGAAACTT CTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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KEG cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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