BAK1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	BAK1
Entrez Gene ID	829480
Full Name	BRI1-associated receptor kinase
Synonyms	ATBAK1,ATSERK3,BRI1-associated receptor kinase,ELG,ELONGATED,F17M5.190,F17M5_190,RECEPTOR KINASES LIKE SERK 10,RKS10,SERK3,SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 3
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001203975.1	NP_001190904.1	BRI1-associated receptor kinase
NM_119497.5	NP_567920.1	BRI1-associated receptor kinase

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	SERK4	NP_178999.2
Arabidopsis thaliana (thale cress)	BAK1	NP_001190904.1
Arabidopsis thaliana (thale cress)	SERK5	NP_179000.3

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

an allosteric mechanism for inhibition of BAK1 by C408 S-glutathionylation
Title: Allosteric Control of a Plant Receptor Kinase through S-Glutathionylation.

These findings show that BAK1 contains an additional AtRALF1 binding site, indicating that this protein may be part of a AtRALF1-containing complex as a co-receptor, and it is required for the negative regulation of cell expansion
Title: BAK1 is involved in AtRALF1-induced inhibition of root cell expansion.

The found that in an otherwise "active" BAK1 the alphaC helix is highly disordered, a hallmark of deactivation, whereas the BRI1 alphaC helix is moderately disordered and displays swinging behavior similar to numerous animal kinases.
Title: Molecular dynamics simulations reveal the conformational dynamics of

Study identifies a link between plant perception of biotic threats by BAK1, cellular calcium entry mediated by GLRs, and intracellular calcium release by TPC1 during a biologically relevant interaction.
Title: Interplay of Plasma Membrane and Vacuolar Ion Channels, Together with BAK1, Elicits Rapid Cytosolic Calcium Elevations in Arabidopsis during Aphid Feeding.

These data suggest that BIK1 cooperates with BAK1 to regulate constitutive immunity and cell death.
Title: BIK1 cooperates with BAK1 to regulate constitutive immunity and cell death in Arabidopsis.

The following BAK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BAK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb17065	NM_119497.5 Latest version!	Arabidopsis thaliana BRI1-associated receptor kinase (BAK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OAb30198	NM_001203975.1 Latest version!	Arabidopsis thaliana BRI1-associated receptor kinase (BAK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BAK1 ( NM_119497.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_567920.1 Arabidopsis thaliana BRI1-associated receptor kinase (BAK1), mRNA.

Price & Availability↑

CloneID	OAb17065
Clone ID Related Accession (Same CDS sequence)	NM_119497.5
Accession Version	NM_119497.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	BRI1-associated receptor kinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_119497.4.

ATGGAACGAA GATTAATGAT CCCTTGCTTC TTTTGGTTGA TTCTCGTTTT GGATTTGGTT 
CTCAGAGTCT CGGGCAACGC CGAAGGTGAT GCTCTAAGTG CACTGAAAAA CAGTTTAGCC 
GACCCTAATA AGGTGCTTCA AAGTTGGGAT GCTACTCTTG TTACTCCATG TACATGGTTT 
CATGTTACTT GCAATAGCGA CAATAGTGTT ACACGTGTTG ACCTTGGGAA TGCAAATCTA 
TCTGGACAGC TCGTAATGCA ACTTGGTCAG CTTCCAAACT TGCAGTACTT GGAGCTTTAT 
AGCAATAACA TTACTGGGAC AATCCCAGAA CAGCTTGGAA ATCTGACGGA ATTGGTGAGC 
TTGGATCTTT ACTTGAACAA TTTAAGCGGG CCTATTCCAT CAACTCTCGG CCGACTTAAG 
AAACTCCGTT TCTTGCGTCT TAATAACAAT AGCTTATCTG GAGAAATTCC AAGGTCTTTG 
ACTGCTGTCC TGACGCTACA AGTTCTGGAT CTCTCAAACA ATCCTCTCAC CGGAGATATT 
CCTGTTAATG GTTCCTTTTC ACTTTTCACT CCAATCAGTT TTGCCAACAC CAAGTTGACT 
CCCCTTCCTG CATCTCCACC GCCTCCTATC TCTCCTACAC CGCCATCACC TGCAGGGAGT 
AATAGAATTA CTGGAGCGAT TGCGGGAGGA GTTGCTGCAG GTGCTGCACT TCTATTTGCT 
GTTCCGGCCA TTGCACTAGC TTGGTGGCGA AGGAAAAAGC CGCAGGACCA CTTCTTTGAT 
GTACCAGCTG AAGAGGACCC AGAAGTTCAT TTAGGACAAC TGAAGAGGTT TTCATTGCGT 
GAACTACAAG TTGCTTCGGA TAATTTTAGC AACAAGAACA TATTGGGTAG AGGTGGTTTT 
GGTAAAGTTT ATAAAGGACG GTTAGCTGAT GGTACTTTAG TGGCCGTTAA AAGGCTAAAA 
GAGGAGCGCA CCCAAGGTGG CGAACTGCAG TTCCAGACAG AGGTTGAGAT GATTAGTATG 
GCGGTTCACA GAAACTTGCT TCGGCTTCGT GGATTTTGCA TGACTCCAAC CGAAAGATTG 
CTTGTTTATC CCTACATGGC TAATGGAAGT GTTGCCTCCT GTTTAAGAGA ACGTCCCGAG 
TCCCAGCCAC CACTTGATTG GCCAAAGAGA CAGCGTATTG CGTTGGGATC TGCAAGAGGG 
CTTGCGTATT TACATGATCA TTGCGACCCA AAGATTATTC ATCGAGATGT GAAAGCTGCA 
AATATTTTGT TGGATGAAGA GTTTGAAGCC GTGGTTGGGG ATTTTGGACT TGCAAAACTC 
ATGGACTACA AAGACACACA TGTGACAACC GCAGTGCGTG GGACAATTGG TCATATAGCC 
CCTGAGTACC TTTCCACTGG AAAATCATCA GAGAAAACCG ATGTCTTTGG GTATGGAGTC 
ATGCTTCTTG AGCTTATCAC TGGACAAAGG GCTTTTGATC TTGCTCGCCT CGCGAATGAT 
GATGATGTCA TGTTACTAGA CTGGGTGAAA GGGTTGTTAA AAGAGAAGAA ATTGGAAGCA 
CTAGTAGATG TTGATCTTCA GGGTAATTAC AAAGACGAAG AAGTGGAGCA GCTAATCCAA 
GTGGCTTTAC TCTGCACTCA GAGTTCACCA ATGGAAAGAC CCAAAATGTC TGAAGTTGTA 
AGAATGCTTG AAGGAGATGG TTTAGCTGAG AGATGGGAAG AGTGGCAAAA GGAGGAAATG 
TTCAGACAAG ATTTCAACTA CCCAACCCAC CATCCAGCCG TGTCTGGCTG GATCATTGGC 
GATTCCACTT CCCAGATCGA AAACGAATAC CCCTCGGGTC CAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_567920.1
CDS	545..2392
Translation	MERRLMIPCFFWLILVLDLVLRVSGNAEGDALSALKNSLADPNKVLQSWDATLVTPCTWFHVTCNSDNSVTRVDLGNANLSGQLVMQLGQLPNLQYLELYSNNITGTIPEQLGNLTELVSLDLYLNNLSGPIPSTLGRLKKLRFLRLNNNSLSGEIPRSLTAVLTLQVLDLSNNPLTGDIPVNGSFSLFTPISFANTKLTPLPASPPPPISPTPPSPAGSNRITGAIAGGVAAGAALLFAVPAIALAWWRRKKPQDHFFDVPAEEDPEVHLGQLKRFSLRELQVASDNFSNKNILGRGGFGKVYKGRLADGTLVAVKRLKEERTQGGELQFQTEVEMISMAVHRNLLRLRGFCMTPTERLLVYPYMANGSVASCLRERPESQPPLDWPKRQRIALGSARGLAYLHDHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLMDYKDTHVTTAVRGTIGHIAPEYLSTGKSSEKTDVFGYGVMLLELITGQRAFDLARLANDDDVMLLDWVKGLLKEKKLEALVDVDLQGNYKDEEVEQLIQVALLCTQSSPMERPKMSEVVRMLEGDGLAERWEEWQKEEMFRQDFNYPTHHPAVSGWIIGDSTSQIENEYPSGPR

Target ORF information:

RefSeq Version	NM_119497.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana BRI1-associated receptor kinase (BAK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_119497.5

ORF Insert Sequence:

ATGGAACGAA GATTAATGAT CCCTTGCTTC TTTTGGTTGA TTCTCGTTTT GGATTTGGTT 
CTCAGAGTCT CGGGCAACGC CGAAGGTGAT GCTCTAAGTG CACTGAAAAA CAGTTTAGCC 
GACCCTAATA AGGTGCTTCA AAGTTGGGAT GCTACTCTTG TTACTCCATG TACATGGTTT 
CATGTTACTT GCAATAGCGA CAATAGTGTT ACACGTGTTG ACCTTGGGAA TGCAAATCTA 
TCTGGACAGC TCGTAATGCA ACTTGGTCAG CTTCCAAACT TGCAGTACTT GGAGCTTTAT 
AGCAATAACA TTACTGGGAC AATCCCAGAA CAGCTTGGAA ATCTGACGGA ATTGGTGAGC 
TTGGATCTTT ACTTGAACAA TTTAAGCGGG CCTATTCCAT CAACTCTCGG CCGACTTAAG 
AAACTCCGTT TCTTGCGTCT TAATAACAAT AGCTTATCTG GAGAAATTCC AAGGTCTTTG 
ACTGCTGTCC TGACGCTACA AGTTCTGGAT CTCTCAAACA ATCCTCTCAC CGGAGATATT 
CCTGTTAATG GTTCCTTTTC ACTTTTCACT CCAATCAGTT TTGCCAACAC CAAGTTGACT 
CCCCTTCCTG CATCTCCACC GCCTCCTATC TCTCCTACAC CGCCATCACC TGCAGGGAGT 
AATAGAATTA CTGGAGCGAT TGCGGGAGGA GTTGCTGCAG GTGCTGCACT TCTATTTGCT 
GTTCCGGCCA TTGCACTAGC TTGGTGGCGA AGGAAAAAGC CGCAGGACCA CTTCTTTGAT 
GTACCAGCTG AAGAGGACCC AGAAGTTCAT TTAGGACAAC TGAAGAGGTT TTCATTGCGT 
GAACTACAAG TTGCTTCGGA TAATTTTAGC AACAAGAACA TATTGGGTAG AGGTGGTTTT 
GGTAAAGTTT ATAAAGGACG GTTAGCTGAT GGTACTTTAG TGGCCGTTAA AAGGCTAAAA 
GAGGAGCGCA CCCAAGGTGG CGAACTGCAG TTCCAGACAG AGGTTGAGAT GATTAGTATG 
GCGGTTCACA GAAACTTGCT TCGGCTTCGT GGATTTTGCA TGACTCCAAC CGAAAGATTG 
CTTGTTTATC CCTACATGGC TAATGGAAGT GTTGCCTCCT GTTTAAGAGA ACGTCCCGAG 
TCCCAGCCAC CACTTGATTG GCCAAAGAGA CAGCGTATTG CGTTGGGATC TGCAAGAGGG 
CTTGCGTATT TACATGATCA TTGCGACCCA AAGATTATTC ATCGAGATGT GAAAGCTGCA 
AATATTTTGT TGGATGAAGA GTTTGAAGCC GTGGTTGGGG ATTTTGGACT TGCAAAACTC 
ATGGACTACA AAGACACACA TGTGACAACC GCAGTGCGTG GGACAATTGG TCATATAGCC 
CCTGAGTACC TTTCCACTGG AAAATCATCA GAGAAAACCG ATGTCTTTGG GTATGGAGTC 
ATGCTTCTTG AGCTTATCAC TGGACAAAGG GCTTTTGATC TTGCTCGCCT CGCGAATGAT 
GATGATGTCA TGTTACTAGA CTGGGTGAAA GGGTTGTTAA AAGAGAAGAA ATTGGAAGCA 
CTAGTAGATG TTGATCTTCA GGGTAATTAC AAAGACGAAG AAGTGGAGCA GCTAATCCAA 
GTGGCTTTAC TCTGCACTCA GAGTTCACCA ATGGAAAGAC CCAAAATGTC TGAAGTTGTA 
AGAATGCTTG AAGGAGATGG TTTAGCTGAG AGATGGGAAG AGTGGCAAAA GGAGGAAATG 
TTCAGACAAG ATTTCAACTA CCCAACCCAC CATCCAGCCG TGTCTGGCTG GATCATTGGC 
GATTCCACTT CCCAGATCGA AAACGAATAC CCCTCGGGTC CAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BAK1 ( NM_001203975.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001190904.1 Arabidopsis thaliana BRI1-associated receptor kinase (BAK1), mRNA.

Price & Availability↑

CloneID	OAb30198
Clone ID Related Accession (Same CDS sequence)	NM_001203975.1
Accession Version	NM_001203975.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	BRI1-associated receptor kinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGGAACGAA GATTAATGAT CCCTTGCTTC TTTTGGTTGA TTCTCGTTTT GGATTTGGTT 
CTCAGAGTCT CGGGCAACGC CGAAGGTGAT GCTCTAAGTG CACTGAAAAA CAGTTTAGCC 
GACCCTAATA AGGTGCTTCA AAGTTGGGAT GCTACTCTTG TTACTCCATG TACATGGTTT 
CATGTTACTT GCAATAGCGA CAATAGTGTT ACACGTGTTG ACCTTGGGAA TGCAAATCTA 
TCTGGACAGC TCGTAATGCA ACTTGGTCAG CTTCCAAACT TGCAGTACTT GGAGCTTTAT 
AGCAATAACA TTACTGGGAC AATCCCAGAA CAGCTTGGAA ATCTGACGGA ATTGGTGAGC 
TTGGATCTTT ACTTGAACAA TTTAAGCGGG CCTATTCCAT CAACTCTCGG CCGACTTAAG 
AAACTCCGTT TCTTGAGTCA GAAAGTAGTG TCGCCAAATA GATGCTACGT TATTTTACTA 
GATGAAAAGG TGTTCTCTTG GAGACTAGGA TGTTGTATAA TATGGTCTAT TTTAATTATG 
AGCTTTCGTA AAAGGAATCA GAACTCTATC CTTGTGCGTC TTAATAACAA TAGCTTATCT 
GGAGAAATTC CAAGGTCTTT GACTGCTGTC CTGACGCTAC AAGTTCTGGA TCTCTCAAAC 
AATCCTCTCA CCGGAGATAT TCCTGTTAAT GGTTCCTTTT CACTTTTCAC TCCAATCAGT 
TTTGCCAACA CCAAGTTGAC TCCCCTTCCT GCATCTCCAC CGCCTCCTAT CTCTCCTACA 
CCGCCATCAC CTGCAGGGAG TAATAGAATT ACTGGAGCGA TTGCGGGAGG AGTTGCTGCA 
GGTGCTGCAC TTCTATTTGC TGTTCCGGCC ATTGCACTAG CTTGGTGGCG AAGGAAAAAG 
CCGCAGGACC ACTTCTTTGA TGTACCAGCT GAAGAGGACC CAGAAGTTCA TTTAGGACAA 
CTGAAGAGGT TTTCATTGCG TGAACTACAA GTTGCTTCGG ATAATTTTAG CAACAAGAAC 
ATATTGGGTA GAGGTGGTTT TGGTAAAGTT TATAAAGGAC GGTTAGCTGA TGGTACTTTA 
GTGGCCGTTA AAAGGCTAAA AGAGGAGCGC ACCCAAGGTG GCGAACTGCA GTTCCAGACA 
GAGGTTGAGA TGATTAGTAT GGCGGTTCAC AGAAACTTGC TTCGGCTTCG TGGATTTTGC 
ATGACTCCAA CCGAAAGATT GCTTGTTTAT CCCTACATGG CTAATGGAAG TGTTGCCTCC 
TGTTTAAGAG AACGTCCCGA GTCCCAGCCA CCACTTGATT GGCCAAAGAG ACAGCGTATT 
GCGTTGGGAT CTGCAAGAGG GCTTGCGTAT TTACATGATC ATTGCGACCC AAAGATTATT 
CATCGAGATG TGAAAGCTGC AAATATTTTG TTGGATGAAG AGTTTGAAGC CGTGGTTGGG 
GATTTTGGAC TTGCAAAACT CATGGACTAC AAAGACACAC ATGTGACAAC CGCAGTGCGT 
GGGACAATTG GTCATATAGC CCCTGAGTAC CTTTCCACTG GAAAATCATC AGAGAAAACC 
GATGTCTTTG GGTATGGAGT CATGCTTCTT GAGCTTATCA CTGGACAAAG GGCTTTTGAT 
CTTGCTCGCC TCGCGAATGA TGATGATGTC ATGTTACTAG ACTGGGTGAA AGGGTTGTTA 
AAAGAGAAGA AATTGGAAGC ACTAGTAGAT GTTGATCTTC AGGGTAATTA CAAAGACGAA 
GAAGTGGAGC AGCTAATCCA AGTGGCTTTA CTCTGCACTC AGAGTTCACC AATGGAAAGA 
CCCAAAATGT CTGAAGTTGT AAGAATGCTT GAAGGAGATG GTTTAGCTGA GAGATGGGAA 
GAGTGGCAAA AGGAGGAAAT GTTCAGACAA GATTTCAACT ACCCAACCCA CCATCCAGCC 
GTGTCTGGCT GGATCATTGG CGATTCCACT TCCCAGATCG AAAACGAATA CCCCTCGGGT 
CCAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001190904.1
CDS	490..2478
Translation	MERRLMIPCFFWLILVLDLVLRVSGNAEGDALSALKNSLADPNKVLQSWDATLVTPCTWFHVTCNSDNSVTRVDLGNANLSGQLVMQLGQLPNLQYLELYSNNITGTIPEQLGNLTELVSLDLYLNNLSGPIPSTLGRLKKLRFLSQKVVSPNRCYVILLDEKVFSWRLGCCIIWSILIMSFRKRNQNSILVRLNNNSLSGEIPRSLTAVLTLQVLDLSNNPLTGDIPVNGSFSLFTPISFANTKLTPLPASPPPPISPTPPSPAGSNRITGAIAGGVAAGAALLFAVPAIALAWWRRKKPQDHFFDVPAEEDPEVHLGQLKRFSLRELQVASDNFSNKNILGRGGFGKVYKGRLADGTLVAVKRLKEERTQGGELQFQTEVEMISMAVHRNLLRLRGFCMTPTERLLVYPYMANGSVASCLRERPESQPPLDWPKRQRIALGSARGLAYLHDHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLMDYKDTHVTTAVRGTIGHIAPEYLSTGKSSEKTDVFGYGVMLLELITGQRAFDLARLANDDDVMLLDWVKGLLKEKKLEALVDVDLQGNYKDEEVEQLIQVALLCTQSSPMERPKMSEVVRMLEGDGLAERWEEWQKEEMFRQDFNYPTHHPAVSGWIIGDSTSQIENEYPSGPR

Target ORF information:

RefSeq Version	NM_001203975.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana BRI1-associated receptor kinase (BAK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001203975.1

ORF Insert Sequence:

ATGGAACGAA GATTAATGAT CCCTTGCTTC TTTTGGTTGA TTCTCGTTTT GGATTTGGTT 
CTCAGAGTCT CGGGCAACGC CGAAGGTGAT GCTCTAAGTG CACTGAAAAA CAGTTTAGCC 
GACCCTAATA AGGTGCTTCA AAGTTGGGAT GCTACTCTTG TTACTCCATG TACATGGTTT 
CATGTTACTT GCAATAGCGA CAATAGTGTT ACACGTGTTG ACCTTGGGAA TGCAAATCTA 
TCTGGACAGC TCGTAATGCA ACTTGGTCAG CTTCCAAACT TGCAGTACTT GGAGCTTTAT 
AGCAATAACA TTACTGGGAC AATCCCAGAA CAGCTTGGAA ATCTGACGGA ATTGGTGAGC 
TTGGATCTTT ACTTGAACAA TTTAAGCGGG CCTATTCCAT CAACTCTCGG CCGACTTAAG 
AAACTCCGTT TCTTGAGTCA GAAAGTAGTG TCGCCAAATA GATGCTACGT TATTTTACTA 
GATGAAAAGG TGTTCTCTTG GAGACTAGGA TGTTGTATAA TATGGTCTAT TTTAATTATG 
AGCTTTCGTA AAAGGAATCA GAACTCTATC CTTGTGCGTC TTAATAACAA TAGCTTATCT 
GGAGAAATTC CAAGGTCTTT GACTGCTGTC CTGACGCTAC AAGTTCTGGA TCTCTCAAAC 
AATCCTCTCA CCGGAGATAT TCCTGTTAAT GGTTCCTTTT CACTTTTCAC TCCAATCAGT 
TTTGCCAACA CCAAGTTGAC TCCCCTTCCT GCATCTCCAC CGCCTCCTAT CTCTCCTACA 
CCGCCATCAC CTGCAGGGAG TAATAGAATT ACTGGAGCGA TTGCGGGAGG AGTTGCTGCA 
GGTGCTGCAC TTCTATTTGC TGTTCCGGCC ATTGCACTAG CTTGGTGGCG AAGGAAAAAG 
CCGCAGGACC ACTTCTTTGA TGTACCAGCT GAAGAGGACC CAGAAGTTCA TTTAGGACAA 
CTGAAGAGGT TTTCATTGCG TGAACTACAA GTTGCTTCGG ATAATTTTAG CAACAAGAAC 
ATATTGGGTA GAGGTGGTTT TGGTAAAGTT TATAAAGGAC GGTTAGCTGA TGGTACTTTA 
GTGGCCGTTA AAAGGCTAAA AGAGGAGCGC ACCCAAGGTG GCGAACTGCA GTTCCAGACA 
GAGGTTGAGA TGATTAGTAT GGCGGTTCAC AGAAACTTGC TTCGGCTTCG TGGATTTTGC 
ATGACTCCAA CCGAAAGATT GCTTGTTTAT CCCTACATGG CTAATGGAAG TGTTGCCTCC 
TGTTTAAGAG AACGTCCCGA GTCCCAGCCA CCACTTGATT GGCCAAAGAG ACAGCGTATT 
GCGTTGGGAT CTGCAAGAGG GCTTGCGTAT TTACATGATC ATTGCGACCC AAAGATTATT 
CATCGAGATG TGAAAGCTGC AAATATTTTG TTGGATGAAG AGTTTGAAGC CGTGGTTGGG 
GATTTTGGAC TTGCAAAACT CATGGACTAC AAAGACACAC ATGTGACAAC CGCAGTGCGT 
GGGACAATTG GTCATATAGC CCCTGAGTAC CTTTCCACTG GAAAATCATC AGAGAAAACC 
GATGTCTTTG GGTATGGAGT CATGCTTCTT GAGCTTATCA CTGGACAAAG GGCTTTTGAT 
CTTGCTCGCC TCGCGAATGA TGATGATGTC ATGTTACTAG ACTGGGTGAA AGGGTTGTTA 
AAAGAGAAGA AATTGGAAGC ACTAGTAGAT GTTGATCTTC AGGGTAATTA CAAAGACGAA 
GAAGTGGAGC AGCTAATCCA AGTGGCTTTA CTCTGCACTC AGAGTTCACC AATGGAAAGA 
CCCAAAATGT CTGAAGTTGT AAGAATGCTT GAAGGAGATG GTTTAGCTGA GAGATGGGAA 
GAGTGGCAAA AGGAGGAAAT GTTCAGACAA GATTTCAACT ACCCAACCCA CCATCCAGCC 
GTGTCTGGCT GGATCATTGG CGATTCCACT TCCCAGATCG AAAACGAATA CCCCTCGGGT 
CCAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
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BAK1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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