AT4G31230 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AT4G31230
Entrez Gene ID	829250
Full Name	kinase with adenine nucleotide alpha hydrolases-like domain-containing protein
Synonyms	F8F16.50,F8F16_50
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_119272.3	NP_194851.2	kinase with adenine nucleotide alpha hydrolases-like domain-containing protein
NM_001342067.1	NP_001328658.1	kinase with adenine nucleotide alpha hydrolases-like domain-containing protein

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os02g0787200	NP_001048340.1
Arabidopsis thaliana (thale cress)	AT2G24370	NP_180014.2
Arabidopsis thaliana (thale cress)	AT4G31230	NP_194851.2

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AT4G31230 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT4G31230 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb16831	NM_119272.3 Latest version!	Arabidopsis thaliana kinase with adenine nucleotide alpha hydrolases-like domain-containing protein (AT4G31230), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb39896	NM_001342067.1 Latest version!	Arabidopsis thaliana kinase with adenine nucleotide alpha hydrolases-like domain-containing protein (AT4G31230), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AT4G31230 ( NM_119272.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_194851.2 Arabidopsis thaliana kinase with adenine nucleotide alpha hydrolases-like domain-containing protein (AT4G31230), mRNA.

Price & Availability↑

CloneID	OAb16831
Clone ID Related Accession (Same CDS sequence)	NM_119272.3
Accession Version	NM_119272.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	kinase with adenine nucleotide alpha hydrolases-like domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_119272.2.

ATGGCGAAGA ACAGGAGGGA CAGAGACGGA GGATTTGGCG TGGATGGATT GGTTGCTGTG 
GCGATAGACA GAGACAAAAA CAGCCAAGGT GCTCTGAAAT GGGCTGTTGA TAATTTGTTG 
CAGAAAGGCC AAACCGTAGT CCTCGTACAT GTCAAGCCTC GGGCTTCATC TTTGTCCACA 
AACCCTTCAA TCAACTCAAA CTCTTCTAAG ACGAGCCAAA TAAATGGTGA TTCTTCATTG 
GTGTGTGGAG AACCAGAAGG CTCTTATAAA CAGTTGTTTC TTCCTTTTCG TTGCCTCTGT 
TCACGCAAAG ATATACAATG CAAAGATGTG TTATTGGAGG AGTCAGATGT GGCGAGAGCT 
TTAGTCGAAT ACGCAAATCA GGTCATGATT GAGGTATTGG TGGTTGGTTC CTCCTCGAAA 
GGTGGCTTTC TCAGGTTCAA CAAACCTATT GATATTCCAG GAGCCATCAC CAAGACTGCA 
CCTGATTTCT GCACGGTGTA TGCCATAACC AAAGGAAAGC TTTCAACAAA GAAAACAGCT 
TCTCGTGCTG CGCCGTCTGT TTCTCCATTA CGCATCCAGC TTCAACAGAA TGGCTTAAAG 
CCACATCCAC CTTTGCCTTC TGCAACTACT AACACAAGAG CTGAGAGACA GTCATTTGAG 
TCTCAGCATC GCAGGTCCCT TGACGATCAG TCAGATTCCT TCAGGCCACC CTACAATAAA 
AGAGGCTTGA CTGGGAGATC ATATGGAGAA CTATCAATCC CGGATTCTGA AATATCCTTC 
AACAGCTCTG GAAGACCAAG CATAGAGCGC AACTCTCCTT CCTTATATGA CAATTCGGAT 
CCAAACCGGA CTCCTCCAAG GCTATCAAAT TTCTCAGACG TTGACTATTG TAGCTTCGAG 
TCAATGACCT TCGGAAGGAG GTCAATGGAT TTGAGCTCAC CAACTGCTTT CTCAACTGGC 
TCATTTGAGA ATGAAAGGTT TTCATCGGCT TCTCAAGGAG GGGATGATGT AGAGGCTGAG 
ATGAGGAGGC TTAAACTGGA GCTGAAACAA ACGATGGAAA TGTACAGTAC AGCCTGTAAG 
GAAGCTCTCA CAGCAAAACA TAAGGCAACA GAGCTTCAAC GTTGGAAGTT AGCAGAAGAA 
CGGAAATTCG AAGAAGCAAA GCTTGCAGAG GAAGCTGCAT TAGCCATTGC GGAGAAGGAG 
AAAGCAAAGT CCAAAGCAGC AATGGAAGCA GCTGAGGCAG CTCAAAGGAT TGCTGATATC 
GAGTCCCGAA AGAGAGTCGA TGCAGAAACG AAAGCTCTGA AGGAATCAGA GGCGAGAACA 
AAAGCCGTGA ATGCTTTAGC AAAGGATGTC AGATACAGGA AGTACTCCAT TGAGGAGATT 
GAGGATGCAA CAGAGTTCTT TGATGATAAA TACAAGATCG GAGAAGGCAG TTATGGACCT 
GTCTACAAGT GTTATCTGGA TCACACACCT GTGGCTGTCA AAGCTTTGCG CCCAGATGCA 
GCTCAAGGCA GGTCACAGTT TCAGAAAGAG GTTGAAGTGT TATGCAGTAT AAGGCACCCT 
AACATGGTTC TTCTGCTTGG AGCATGCCCA GAATGTGGAT GCTTAGTGTA TGAGTTCATG 
GCTAATGGGA GCTTAGAAGA CCGTCTTTTC AGACAAGGAG ACAGCCCGGC TCTCTCTTGG 
CAAACAAGAT TCAGAATTGC TGCAGAGATC GGCACTGTAC TGTTGTTCCT CCACCAGACT 
AAACCAGAAC CACTGGTTCA CCGTGATCTC AAACCAGCAA ACATCTTACT AGACCGCAAC 
TTTGTCAGCA AGCTTGCTGA TGTTGGCTTG GCTAGGCTGG TCCCTCCATC CGTTGCAAAC 
ACCGTGACGC AATACCACAT GACATCAACT GCTGGCACGT TTTGCTACAT CGACCCAGAG 
TATCAGCAGA CGGGTATGCT GGGTGTGAAA TCTGACATTT ATTCGCTTGG TATTATGTTT 
CTGCAGTTAA TAACAGGAAA ACCACCTATG GGTCTAACCC ATTATGTCGA AAGAGCTCTC 
GAGAAGGGTA ATTTGAAAGA TTTGCTCGAT CCAGCTGTGT CTGATTGGCC TGTTGAAGAC 
ACCACAGAGT TTGCCAAGTT GGCTCTCAAG TGCGCAGAGA TAAGGCGAAA AGACAGACCT 
GATCTTTCCA AAGTTATATT GCCAGAGCTC AACAGATTAA GAGTACTTGC TGAAGAATCA 
ACACAGGCTG CAGTAGTCAT TAACAGCTCC GGACCAACCC CCACGGAGAG TCAAACTTCC 
TCCCCCAAGT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_194851.2
CDS	184..2478
Translation	MAKNRRDRDGGFGVDGLVAVAIDRDKNSQGALKWAVDNLLQKGQTVVLVHVKPRASSLSTNPSINSNSSKTSQINGDSSLVCGEPEGSYKQLFLPFRCLCSRKDIQCKDVLLEESDVARALVEYANQVMIEVLVVGSSSKGGFLRFNKPIDIPGAITKTAPDFCTVYAITKGKLSTKKTASRAAPSVSPLRIQLQQNGLKPHPPLPSATTNTRAERQSFESQHRRSLDDQSDSFRPPYNKRGLTGRSYGELSIPDSEISFNSSGRPSIERNSPSLYDNSDPNRTPPRLSNFSDVDYCSFESMTFGRRSMDLSSPTAFSTGSFENERFSSASQGGDDVEAEMRRLKLELKQTMEMYSTACKEALTAKHKATELQRWKLAEERKFEEAKLAEEAALAIAEKEKAKSKAAMEAAEAAQRIADIESRKRVDAETKALKESEARTKAVNALAKDVRYRKYSIEEIEDATEFFDDKYKIGEGSYGPVYKCYLDHTPVAVKALRPDAAQGRSQFQKEVEVLCSIRHPNMVLLLGACPECGCLVYEFMANGSLEDRLFRQGDSPALSWQTRFRIAAEIGTVLLFLHQTKPEPLVHRDLKPANILLDRNFVSKLADVGLARLVPPSVANTVTQYHMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITGKPPMGLTHYVERALEKGNLKDLLDPAVSDWPVEDTTEFAKLALKCAEIRRKDRPDLSKVILPELNRLRVLAEESTQAAVVINSSGPTPTESQTSSPKL

Target ORF information:

RefSeq Version	NM_119272.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana kinase with adenine nucleotide alpha hydrolases-like domain-containing protein (AT4G31230), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_119272.3

ORF Insert Sequence:

ATGGCGAAGA ACAGGAGGGA CAGAGACGGA GGATTTGGCG TGGATGGATT GGTTGCTGTG 
GCGATAGACA GAGACAAAAA CAGCCAAGGT GCTCTGAAAT GGGCTGTTGA TAATTTGTTG 
CAGAAAGGCC AAACCGTAGT CCTCGTACAT GTCAAGCCTC GGGCTTCATC TTTGTCCACA 
AACCCTTCAA TCAACTCAAA CTCTTCTAAG ACGAGCCAAA TAAATGGTGA TTCTTCATTG 
GTGTGTGGAG AACCAGAAGG CTCTTATAAA CAGTTGTTTC TTCCTTTTCG TTGCCTCTGT 
TCACGCAAAG ATATACAATG CAAAGATGTG TTATTGGAGG AGTCAGATGT GGCGAGAGCT 
TTAGTCGAAT ACGCAAATCA GGTCATGATT GAGGTATTGG TGGTTGGTTC CTCCTCGAAA 
GGTGGCTTTC TCAGGTTCAA CAAACCTATT GATATTCCAG GAGCCATCAC CAAGACTGCA 
CCTGATTTCT GCACGGTGTA TGCCATAACC AAAGGAAAGC TTTCAACAAA GAAAACAGCT 
TCTCGTGCTG CGCCGTCTGT TTCTCCATTA CGCATCCAGC TTCAACAGAA TGGCTTAAAG 
CCACATCCAC CTTTGCCTTC TGCAACTACT AACACAAGAG CTGAGAGACA GTCATTTGAG 
TCTCAGCATC GCAGGTCCCT TGACGATCAG TCAGATTCCT TCAGGCCACC CTACAATAAA 
AGAGGCTTGA CTGGGAGATC ATATGGAGAA CTATCAATCC CGGATTCTGA AATATCCTTC 
AACAGCTCTG GAAGACCAAG CATAGAGCGC AACTCTCCTT CCTTATATGA CAATTCGGAT 
CCAAACCGGA CTCCTCCAAG GCTATCAAAT TTCTCAGACG TTGACTATTG TAGCTTCGAG 
TCAATGACCT TCGGAAGGAG GTCAATGGAT TTGAGCTCAC CAACTGCTTT CTCAACTGGC 
TCATTTGAGA ATGAAAGGTT TTCATCGGCT TCTCAAGGAG GGGATGATGT AGAGGCTGAG 
ATGAGGAGGC TTAAACTGGA GCTGAAACAA ACGATGGAAA TGTACAGTAC AGCCTGTAAG 
GAAGCTCTCA CAGCAAAACA TAAGGCAACA GAGCTTCAAC GTTGGAAGTT AGCAGAAGAA 
CGGAAATTCG AAGAAGCAAA GCTTGCAGAG GAAGCTGCAT TAGCCATTGC GGAGAAGGAG 
AAAGCAAAGT CCAAAGCAGC AATGGAAGCA GCTGAGGCAG CTCAAAGGAT TGCTGATATC 
GAGTCCCGAA AGAGAGTCGA TGCAGAAACG AAAGCTCTGA AGGAATCAGA GGCGAGAACA 
AAAGCCGTGA ATGCTTTAGC AAAGGATGTC AGATACAGGA AGTACTCCAT TGAGGAGATT 
GAGGATGCAA CAGAGTTCTT TGATGATAAA TACAAGATCG GAGAAGGCAG TTATGGACCT 
GTCTACAAGT GTTATCTGGA TCACACACCT GTGGCTGTCA AAGCTTTGCG CCCAGATGCA 
GCTCAAGGCA GGTCACAGTT TCAGAAAGAG GTTGAAGTGT TATGCAGTAT AAGGCACCCT 
AACATGGTTC TTCTGCTTGG AGCATGCCCA GAATGTGGAT GCTTAGTGTA TGAGTTCATG 
GCTAATGGGA GCTTAGAAGA CCGTCTTTTC AGACAAGGAG ACAGCCCGGC TCTCTCTTGG 
CAAACAAGAT TCAGAATTGC TGCAGAGATC GGCACTGTAC TGTTGTTCCT CCACCAGACT 
AAACCAGAAC CACTGGTTCA CCGTGATCTC AAACCAGCAA ACATCTTACT AGACCGCAAC 
TTTGTCAGCA AGCTTGCTGA TGTTGGCTTG GCTAGGCTGG TCCCTCCATC CGTTGCAAAC 
ACCGTGACGC AATACCACAT GACATCAACT GCTGGCACGT TTTGCTACAT CGACCCAGAG 
TATCAGCAGA CGGGTATGCT GGGTGTGAAA TCTGACATTT ATTCGCTTGG TATTATGTTT 
CTGCAGTTAA TAACAGGAAA ACCACCTATG GGTCTAACCC ATTATGTCGA AAGAGCTCTC 
GAGAAGGGTA ATTTGAAAGA TTTGCTCGAT CCAGCTGTGT CTGATTGGCC TGTTGAAGAC 
ACCACAGAGT TTGCCAAGTT GGCTCTCAAG TGCGCAGAGA TAAGGCGAAA AGACAGACCT 
GATCTTTCCA AAGTTATATT GCCAGAGCTC AACAGATTAA GAGTACTTGC TGAAGAATCA 
ACACAGGCTG CAGTAGTCAT TAACAGCTCC GGACCAACCC CCACGGAGAG TCAAACTTCC 
TCCCCCAAGT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AT4G31230 ( NM_001342067.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001328658.1 Arabidopsis thaliana kinase with adenine nucleotide alpha hydrolases-like domain-containing protein (AT4G31230), mRNA.

Price & Availability↑

CloneID	OAb39896
Clone ID Related Accession (Same CDS sequence)	NM_001342067.1
Accession Version	NM_001342067.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	kinase with adenine nucleotide alpha hydrolases-like domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGGAACATG TTCAGATACA ATGCAAAGAT GTGTTATTGG AGGAGTCAGA TGTGGCGAGA 
GCTTTAGTCG AATACGCAAA TCAGGTCATG ATTGAGGTAT TGGTGGTTGG TTCCTCCTCG 
AAAGGTGGCT TTCTCAGGTT CAACAAACCT ATTGATATTC CAGGAGCCAT CACCAAGACT 
GCACCTGATT TCTGCACGGT GTATGCCATA ACCAAAGGAA AGCTTTCAAC AAAGAAAACA 
GCTTCTCGTG CTGCGCCGTC TGTTTCTCCA TTACGCATCC AGCTTCAACA GAATGGCTTA 
AAGCCACATC CACCTTTGCC TTCTGCAACT ACTAACACAA GAGCTGAGAG ACAGTCATTT 
GAGTCTCAGC ATCGCAGGTC CCTTGACGAT CAGTCAGATT CCTTCAGGCC ACCCTACAAT 
AAAAGAGGCT TGACTGGGAG ATCATATGGA GAACTATCAA TCCCGGATTC TGAAATATCC 
TTCAACAGCT CTGGAAGACC AAGCATAGAG CGCAACTCTC CTTCCTTATA TGACAATTCG 
GATCCAAACC GGACTCCTCC AAGGCTATCA AATTTCTCAG ACGTTGACTA TTGTAGCTTC 
GAGTCAATGA CCTTCGGAAG GAGGTCAATG GATTTGAGCT CACCAACTGC TTTCTCAACT 
GGCTCATTTG AGAATGAAAG GTTTTCATCG GCTTCTCAAG GAGGGGATGA TGTAGAGGCT 
GAGATGAGGA GGCTTAAACT GGAGCTGAAA CAAACGATGG AAATGTACAG TACAGCCTGT 
AAGGAAGCTC TCACAGCAAA ACATAAGGCA ACAGAGCTTC AACGTTGGAA GTTAGCAGAA 
GAACGGAAAT TCGAAGAAGC AAAGCTTGCA GAGGAAGCTG CATTAGCCAT TGCGGAGAAG 
GAGAAAGCAA AGTCCAAAGC AGCAATGGAA GCAGCTGAGG CAGCTCAAAG GATTGCTGAT 
ATCGAGTCCC GAAAGAGAGT CGATGCAGAA ACGAAAGCTC TGAAGGAATC AGAGGCGAGA 
ACAAAAGCCG TGAATGCTTT AGCAAAGGAT GTCAGATACA GGAAGTACTC CATTGAGGAG 
ATTGAGGATG CAACAGAGTT CTTTGATGAT AAATACAAGA TCGGAGAAGG CAGTTATGGA 
CCTGTCTACA AGTGTTATCT GGATCACACA CCTGTGGCTG TCAAAGCTTT GCGCCCAGAT 
GCAGCTCAAG GCAGGTCACA GTTTCAGAAA GAGGTTGAAG TGTTATGCAG TATAAGGCAC 
CCTAACATGG TTCTTCTGCT TGGAGCATGC CCAGAATGTG GATGCTTAGT GTATGAGTTC 
ATGGCTAATG GGAGCTTAGA AGACCGTCTT TTCAGACAAG GAGACAGCCC GGCTCTCTCT 
TGGCAAACAA GATTCAGAAT TGCTGCAGAG ATCGGCACTG TACTGTTGTT CCTCCACCAG 
ACTAAACCAG AACCACTGGT TCACCGTGAT CTCAAACCAG CAAACATCTT ACTAGACCGC 
AACTTTGTCA GCAAGCTTGC TGATGTTGGC TTGGCTAGGC TGGTCCCTCC ATCCGTTGCA 
AACACCGTGA CGCAATACCA CATGACATCA ACTGCTGGCA CGTTTTGCTA CATCGACCCA 
GAGTATCAGC AGACGGGTAT GCTGGGTGTG AAATCTGACA TTTATTCGCT TGGTATTATG 
TTTCTGCAGT TAATAACAGG AAAACCACCT ATGGGTCTAA CCCATTATGT CGAAAGAGCT 
CTCGAGAAGG GTAATTTGAA AGATTTGCTC GATCCAGCTG TGTCTGATTG GCCTGTTGAA 
GACACCACAG AGTTTGCCAA GTTGGCTCTC AAGTGCGCAG AGATAAGGCG AAAAGACAGA 
CCTGATCTTT CCAAAGTTAT ATTGCCAGAG CTCAACAGAT TAAGAGTACT TGCTGAAGAA 
TCAACACAGG CTGCAGTAGT CATTAACAGC TCCGGACCAA CCCCCACGGA GAGTCAAACT 
TCCTCCCCCA AGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001328658.1
CDS	522..2519
Translation	MEHVQIQCKDVLLEESDVARALVEYANQVMIEVLVVGSSSKGGFLRFNKPIDIPGAITKTAPDFCTVYAITKGKLSTKKTASRAAPSVSPLRIQLQQNGLKPHPPLPSATTNTRAERQSFESQHRRSLDDQSDSFRPPYNKRGLTGRSYGELSIPDSEISFNSSGRPSIERNSPSLYDNSDPNRTPPRLSNFSDVDYCSFESMTFGRRSMDLSSPTAFSTGSFENERFSSASQGGDDVEAEMRRLKLELKQTMEMYSTACKEALTAKHKATELQRWKLAEERKFEEAKLAEEAALAIAEKEKAKSKAAMEAAEAAQRIADIESRKRVDAETKALKESEARTKAVNALAKDVRYRKYSIEEIEDATEFFDDKYKIGEGSYGPVYKCYLDHTPVAVKALRPDAAQGRSQFQKEVEVLCSIRHPNMVLLLGACPECGCLVYEFMANGSLEDRLFRQGDSPALSWQTRFRIAAEIGTVLLFLHQTKPEPLVHRDLKPANILLDRNFVSKLADVGLARLVPPSVANTVTQYHMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITGKPPMGLTHYVERALEKGNLKDLLDPAVSDWPVEDTTEFAKLALKCAEIRRKDRPDLSKVILPELNRLRVLAEESTQAAVVINSSGPTPTESQTSSPKL

Target ORF information:

RefSeq Version	NM_001342067.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana kinase with adenine nucleotide alpha hydrolases-like domain-containing protein (AT4G31230), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001342067.1

ORF Insert Sequence:

ATGGAACATG TTCAGATACA ATGCAAAGAT GTGTTATTGG AGGAGTCAGA TGTGGCGAGA 
GCTTTAGTCG AATACGCAAA TCAGGTCATG ATTGAGGTAT TGGTGGTTGG TTCCTCCTCG 
AAAGGTGGCT TTCTCAGGTT CAACAAACCT ATTGATATTC CAGGAGCCAT CACCAAGACT 
GCACCTGATT TCTGCACGGT GTATGCCATA ACCAAAGGAA AGCTTTCAAC AAAGAAAACA 
GCTTCTCGTG CTGCGCCGTC TGTTTCTCCA TTACGCATCC AGCTTCAACA GAATGGCTTA 
AAGCCACATC CACCTTTGCC TTCTGCAACT ACTAACACAA GAGCTGAGAG ACAGTCATTT 
GAGTCTCAGC ATCGCAGGTC CCTTGACGAT CAGTCAGATT CCTTCAGGCC ACCCTACAAT 
AAAAGAGGCT TGACTGGGAG ATCATATGGA GAACTATCAA TCCCGGATTC TGAAATATCC 
TTCAACAGCT CTGGAAGACC AAGCATAGAG CGCAACTCTC CTTCCTTATA TGACAATTCG 
GATCCAAACC GGACTCCTCC AAGGCTATCA AATTTCTCAG ACGTTGACTA TTGTAGCTTC 
GAGTCAATGA CCTTCGGAAG GAGGTCAATG GATTTGAGCT CACCAACTGC TTTCTCAACT 
GGCTCATTTG AGAATGAAAG GTTTTCATCG GCTTCTCAAG GAGGGGATGA TGTAGAGGCT 
GAGATGAGGA GGCTTAAACT GGAGCTGAAA CAAACGATGG AAATGTACAG TACAGCCTGT 
AAGGAAGCTC TCACAGCAAA ACATAAGGCA ACAGAGCTTC AACGTTGGAA GTTAGCAGAA 
GAACGGAAAT TCGAAGAAGC AAAGCTTGCA GAGGAAGCTG CATTAGCCAT TGCGGAGAAG 
GAGAAAGCAA AGTCCAAAGC AGCAATGGAA GCAGCTGAGG CAGCTCAAAG GATTGCTGAT 
ATCGAGTCCC GAAAGAGAGT CGATGCAGAA ACGAAAGCTC TGAAGGAATC AGAGGCGAGA 
ACAAAAGCCG TGAATGCTTT AGCAAAGGAT GTCAGATACA GGAAGTACTC CATTGAGGAG 
ATTGAGGATG CAACAGAGTT CTTTGATGAT AAATACAAGA TCGGAGAAGG CAGTTATGGA 
CCTGTCTACA AGTGTTATCT GGATCACACA CCTGTGGCTG TCAAAGCTTT GCGCCCAGAT 
GCAGCTCAAG GCAGGTCACA GTTTCAGAAA GAGGTTGAAG TGTTATGCAG TATAAGGCAC 
CCTAACATGG TTCTTCTGCT TGGAGCATGC CCAGAATGTG GATGCTTAGT GTATGAGTTC 
ATGGCTAATG GGAGCTTAGA AGACCGTCTT TTCAGACAAG GAGACAGCCC GGCTCTCTCT 
TGGCAAACAA GATTCAGAAT TGCTGCAGAG ATCGGCACTG TACTGTTGTT CCTCCACCAG 
ACTAAACCAG AACCACTGGT TCACCGTGAT CTCAAACCAG CAAACATCTT ACTAGACCGC 
AACTTTGTCA GCAAGCTTGC TGATGTTGGC TTGGCTAGGC TGGTCCCTCC ATCCGTTGCA 
AACACCGTGA CGCAATACCA CATGACATCA ACTGCTGGCA CGTTTTGCTA CATCGACCCA 
GAGTATCAGC AGACGGGTAT GCTGGGTGTG AAATCTGACA TTTATTCGCT TGGTATTATG 
TTTCTGCAGT TAATAACAGG AAAACCACCT ATGGGTCTAA CCCATTATGT CGAAAGAGCT 
CTCGAGAAGG GTAATTTGAA AGATTTGCTC GATCCAGCTG TGTCTGATTG GCCTGTTGAA 
GACACCACAG AGTTTGCCAA GTTGGCTCTC AAGTGCGCAG AGATAAGGCG AAAAGACAGA 
CCTGATCTTT CCAAAGTTAT ATTGCCAGAG CTCAACAGAT TAAGAGTACT TGCTGAAGAA 
TCAACACAGG CTGCAGTAGT CATTAACAGC TCCGGACCAA CCCCCACGGA GAGTCAAACT 
TCCTCCCCCA AGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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AT4G31230 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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