TPS4 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	TPS4
Entrez Gene ID	828864
Full Name	trehalose-6-phosphatase synthase S4
Synonyms	ARABIDOPSIS THALIANA TREHALOSE PHOSPHATASE/SYNTHASE 4,ATTPS4,T29A15.40,T29A15_40,trehalose-6-phosphatase synthase S4
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_118890.2	NP_194485.1	trehalose-6-phosphatase synthase S4
NM_001341861.1	NP_001327998.1	trehalose-6-phosphatase synthase S4

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	TPS1	NP_009684.1
Eremothecium gossypii	AGOS_ADL378W	NP_983718.1
Schizosaccharomyces pombe (fission yeast)	tps1	NP_594205.1
Magnaporthe oryzae (rice blast fungus)	MGG_03860	XP_003720031.1
Arabidopsis thaliana (thale cress)	TPS3	NP_173143.4
Arabidopsis thaliana (thale cress)	TPS4	NP_194485.1
Neurospora crassa	NCU09715	XP_958954.1
Arabidopsis thaliana (thale cress)	TPS2	NP_173142.1
Kluyveromyces lactis	KLLA0B08822g	XP_451921.1
Arabidopsis thaliana (thale cress)	TPS1	NP_177979.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that Arabidopsis thaliana exhibits three active trehalose-6-phosphate synthase isoforms: TPS1, TPS2, and TPS4.
Title: Trehalose-6-phosphate synthase 1 is not the only active TPS in Arabidopsis thaliana.

The following TPS4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TPS4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb16476	NM_118890.2 Latest version!	Arabidopsis thaliana trehalose-6-phosphatase synthase S4 (TPS4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb39791	NM_001341861.1 Latest version!	Arabidopsis thaliana trehalose-6-phosphatase synthase S4 (TPS4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TPS4 ( NM_118890.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_194485.1 Arabidopsis thaliana trehalose-6-phosphatase synthase S4 (TPS4), partial mRNA.

Price & Availability↑

CloneID	OAb16476
Clone ID Related Accession (Same CDS sequence)	NM_118890.2
Accession Version	NM_118890.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	trehalose-6-phosphatase synthase S4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Apr 24, 2008 this sequence version replaced NM_118890.1. COMPLETENESS: incomplete on the 3' end.

ATGGCAAGGC CACGACTGCT TGTAGTGTCG ATGTCGCTTC CGGTCACTGC AAAGAGGACT 
GGAGAAGAGT CTTGGTCTTT CACGATGAGT CCTGGTGGTC TAGTTAGTGC TCTTCTAGGT 
TTGAAAGAAT TTGAGACCAA ATGGATTGGA TGGCCTGGAG TAGATGTTCA TGATGCGATT 
GGCAAAAAGA CACTCTCCAT TACCCTAGCT GAAAAGGGAT GTATCCCAGT GTTTTTGGAA 
GAAGTGTGTG ATCAATACTA TAATGGCTAC TGCAACAATA TTCTGTGGCC GATTTTTCAC 
TACTTGGGAA CGCCACCAGA ATATCGTAAC GATGCAACCA TAACATACCA GTCACAGTAT 
GAAGCATACA AGAAAGCAAA TCAAATTTTC TTTGATGTTG TCAAAGAGCA CTATGAAGAA 
GGAGATGTGG TTTGGTGCCA TGATTATCAT GTTATGCTTC TTCCTCAATA TCTTAAGGAA 
TACAACAGTA AGATGAAAGT TGGATGGTTT CTCCATACAC CATTTCCTTC TTCCGAGATG 
TACAAAACAT TGCCCTCGCG ATCCGATCTG CTTCGTTCTG TTCTTACTGC TGATTTAGTT 
GGTTTCCATA CCTATGACTT CGCAAGACAT TTTTTGAATG CATGCATGTG TATTCTTGGA 
GTCGAAGCAA CATCTGAAGG AATTGTGGAT CAGGGCAAAG TCACTCGAGT AGCTGTTTTT 
CCCATTGGGA TAGAACCTGA GAGGTTTATA AACACAAGTG AACTCTCTGA AGTTGTTCAA 
TACATGAAGA AGTTCAAAAA CGATTTTGGT GGGAGAAAGT TGATATTAGG TGTTGATCGT 
CTCGATACGA TCAAAGGGAT TCCACAAAAA TATCAGGCAT TTGAGAAGTT CTTAGAGGAA 
AATGCAGAGT GGCGTGGTAA AGTCATGTTA CTTCAGATTG CAGTGCCAAC AAGGAACGGT 
ATTGGTGAAT ATCAAAAGAT CAAAGACCAA TGTCATTACC ATGTTGGACG AATTAATGGT 
CGTTTTGGTT CCATCTCTTC TGTTCCAATA ATTCACCTGG ATTGTTCTAT TGACTTTAAT 
CAATTATGCG CACTTTATGC CATCACAGAT GTATTACTTG TAACATCTCT GAGAGATGGA 
ATGAACCTTG TGAGTTCTGA ATTCATTGCC TGCCAAAAAG CAGAAAAAGG GGTTCTCATT 
CTAAGCGAGT TTGCAGGTGC TGGACAGTCA CTTGGTGCCG GAGCTATTTT AGTGAATCCT 
TGGAACATTA AAGAAGTTTC TTCTGCCATT GGAGAAGCTC TAAATATGTC ACATGAAGAA 
AAGGAGAGAA AACATAAAAT CAATTTTCAG TATGTGAAAA CTCACTCCAC TCAGCAGTGG 
GCAGATGATT TCATGAAATT GACTCTTACA AACATTTTAT GCAGTAAACT TATTGAGATT 
ACAACCAGCG CGGAGCTAGG AGCTGGATTA GCCGCCACAC TTGAACTTCC AGAACATGAT 
GTGATTCAAC AATACTCAAA GTCTAACAAT AGACTGCTGA TTCTTGGATT CTATGGAACG 
CTCACTCAGC CGATGAAAAA TCAAGAGAGA AGAGGCGACG GAATGAATCT TGAACTGCAT 
CCACAGCTAA AAGAGAGACT CAAAGAATTA TGCAGTGATC CAAAAACAAC AGTAGTTGTC 
CTGAGTAGAA GTGAAAAATG CATATTGGAT AAAAACTTTG GAGAATATAA CATGTGGTTG 
GCTGCTGAAA ATGGGATGTT TCTAAGGCAT ACAAGTGGAG AATGGGTGAC AAGAATTCCA 
GAACACATGA ATTTGGAATG GATTGATGGT GTTAAGCATG TTTTCAAGTA CTTCACTGAA 
AGAACTCCAG GATCATATCT CGAAACAAGC GAGGCTTCGC TTGTATGGAA CTACGAAAAT 
GCAGATGCTG AATTCGGGAG AGCTCAAGCA AGAGACATGT TACAACACTT GTGGGCAGGA 
CCAATCTCTA ATGCCTCAGT GGATGTTGTT AGAGGAGGCC AATCTGTTGA AGTCCATGCC 
GTTGGTGTGA CAAAGGGAAG TGCAATGGAG CGTATTCTCG GAGAAATAGT GCATAACAAG 
TCAATGGCTA CACCAATTGA CTATGTCTTG TGCATTGGTT GTTTCTTGGG GAAAGACGAA 
GACGTTTACA CATTCTTCGA GCCAGAGTTG ACCAAGAAAG CCAAGTCGCT TTCTTCTTCA 
GGTAGTGACT CCCCCAAGAA GGTATCATCG ACCATTGTTG ACCTCAAGGG AGAGAATTAC 
TTCTCCGTAG CTATTGGACA AACGCACACA AAGGCTCGCT ATTTCCTTGA CTCATCTGAT 
GACGTTGTGA AACTTATTGG CAAGCTTTGC ACACACAACA ATGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_194485.1
CDS	41..2428
Translation	MARPRLLVVSMSLPVTAKRTGEESWSFTMSPGGLVSALLGLKEFETKWIGWPGVDVHDAIGKKTLSITLAEKGCIPVFLEEVCDQYYNGYCNNILWPIFHYLGTPPEYRNDATITYQSQYEAYKKANQIFFDVVKEHYEEGDVVWCHDYHVMLLPQYLKEYNSKMKVGWFLHTPFPSSEMYKTLPSRSDLLRSVLTADLVGFHTYDFARHFLNACMCILGVEATSEGIVDQGKVTRVAVFPIGIEPERFINTSELSEVVQYMKKFKNDFGGRKLILGVDRLDTIKGIPQKYQAFEKFLEENAEWRGKVMLLQIAVPTRNGIGEYQKIKDQCHYHVGRINGRFGSISSVPIIHLDCSIDFNQLCALYAITDVLLVTSLRDGMNLVSSEFIACQKAEKGVLILSEFAGAGQSLGAGAILVNPWNIKEVSSAIGEALNMSHEEKERKHKINFQYVKTHSTQQWADDFMKLTLTNILCSKLIEITTSAELGAGLAATLELPEHDVIQQYSKSNNRLLILGFYGTLTQPMKNQERRGDGMNLELHPQLKERLKELCSDPKTTVVVLSRSEKCILDKNFGEYNMWLAAENGMFLRHTSGEWVTRIPEHMNLEWIDGVKHVFKYFTERTPGSYLETSEASLVWNYENADAEFGRAQARDMLQHLWAGPISNASVDVVRGGQSVEVHAVGVTKGSAMERILGEIVHNKSMATPIDYVLCIGCFLGKDEDVYTFFEPELTKKAKSLSSSGSDSPKKVSSTIVDLKGENYFSVAIGQTHTKARYFLDSSDDVVKLIGKLCTHNNA

Target ORF information:

RefSeq Version	NM_118890.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana trehalose-6-phosphatase synthase S4 (TPS4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_118890.2

ORF Insert Sequence:

ATGGCAAGGC CACGACTGCT TGTAGTGTCG ATGTCGCTTC CGGTCACTGC AAAGAGGACT 
GGAGAAGAGT CTTGGTCTTT CACGATGAGT CCTGGTGGTC TAGTTAGTGC TCTTCTAGGT 
TTGAAAGAAT TTGAGACCAA ATGGATTGGA TGGCCTGGAG TAGATGTTCA TGATGCGATT 
GGCAAAAAGA CACTCTCCAT TACCCTAGCT GAAAAGGGAT GTATCCCAGT GTTTTTGGAA 
GAAGTGTGTG ATCAATACTA TAATGGCTAC TGCAACAATA TTCTGTGGCC GATTTTTCAC 
TACTTGGGAA CGCCACCAGA ATATCGTAAC GATGCAACCA TAACATACCA GTCACAGTAT 
GAAGCATACA AGAAAGCAAA TCAAATTTTC TTTGATGTTG TCAAAGAGCA CTATGAAGAA 
GGAGATGTGG TTTGGTGCCA TGATTATCAT GTTATGCTTC TTCCTCAATA TCTTAAGGAA 
TACAACAGTA AGATGAAAGT TGGATGGTTT CTCCATACAC CATTTCCTTC TTCCGAGATG 
TACAAAACAT TGCCCTCGCG ATCCGATCTG CTTCGTTCTG TTCTTACTGC TGATTTAGTT 
GGTTTCCATA CCTATGACTT CGCAAGACAT TTTTTGAATG CATGCATGTG TATTCTTGGA 
GTCGAAGCAA CATCTGAAGG AATTGTGGAT CAGGGCAAAG TCACTCGAGT AGCTGTTTTT 
CCCATTGGGA TAGAACCTGA GAGGTTTATA AACACAAGTG AACTCTCTGA AGTTGTTCAA 
TACATGAAGA AGTTCAAAAA CGATTTTGGT GGGAGAAAGT TGATATTAGG TGTTGATCGT 
CTCGATACGA TCAAAGGGAT TCCACAAAAA TATCAGGCAT TTGAGAAGTT CTTAGAGGAA 
AATGCAGAGT GGCGTGGTAA AGTCATGTTA CTTCAGATTG CAGTGCCAAC AAGGAACGGT 
ATTGGTGAAT ATCAAAAGAT CAAAGACCAA TGTCATTACC ATGTTGGACG AATTAATGGT 
CGTTTTGGTT CCATCTCTTC TGTTCCAATA ATTCACCTGG ATTGTTCTAT TGACTTTAAT 
CAATTATGCG CACTTTATGC CATCACAGAT GTATTACTTG TAACATCTCT GAGAGATGGA 
ATGAACCTTG TGAGTTCTGA ATTCATTGCC TGCCAAAAAG CAGAAAAAGG GGTTCTCATT 
CTAAGCGAGT TTGCAGGTGC TGGACAGTCA CTTGGTGCCG GAGCTATTTT AGTGAATCCT 
TGGAACATTA AAGAAGTTTC TTCTGCCATT GGAGAAGCTC TAAATATGTC ACATGAAGAA 
AAGGAGAGAA AACATAAAAT CAATTTTCAG TATGTGAAAA CTCACTCCAC TCAGCAGTGG 
GCAGATGATT TCATGAAATT GACTCTTACA AACATTTTAT GCAGTAAACT TATTGAGATT 
ACAACCAGCG CGGAGCTAGG AGCTGGATTA GCCGCCACAC TTGAACTTCC AGAACATGAT 
GTGATTCAAC AATACTCAAA GTCTAACAAT AGACTGCTGA TTCTTGGATT CTATGGAACG 
CTCACTCAGC CGATGAAAAA TCAAGAGAGA AGAGGCGACG GAATGAATCT TGAACTGCAT 
CCACAGCTAA AAGAGAGACT CAAAGAATTA TGCAGTGATC CAAAAACAAC AGTAGTTGTC 
CTGAGTAGAA GTGAAAAATG CATATTGGAT AAAAACTTTG GAGAATATAA CATGTGGTTG 
GCTGCTGAAA ATGGGATGTT TCTAAGGCAT ACAAGTGGAG AATGGGTGAC AAGAATTCCA 
GAACACATGA ATTTGGAATG GATTGATGGT GTTAAGCATG TTTTCAAGTA CTTCACTGAA 
AGAACTCCAG GATCATATCT CGAAACAAGC GAGGCTTCGC TTGTATGGAA CTACGAAAAT 
GCAGATGCTG AATTCGGGAG AGCTCAAGCA AGAGACATGT TACAACACTT GTGGGCAGGA 
CCAATCTCTA ATGCCTCAGT GGATGTTGTT AGAGGAGGCC AATCTGTTGA AGTCCATGCC 
GTTGGTGTGA CAAAGGGAAG TGCAATGGAG CGTATTCTCG GAGAAATAGT GCATAACAAG 
TCAATGGCTA CACCAATTGA CTATGTCTTG TGCATTGGTT GTTTCTTGGG GAAAGACGAA 
GACGTTTACA CATTCTTCGA GCCAGAGTTG ACCAAGAAAG CCAAGTCGCT TTCTTCTTCA 
GGTAGTGACT CCCCCAAGAA GGTATCATCG ACCATTGTTG ACCTCAAGGG AGAGAATTAC 
TTCTCCGTAG CTATTGGACA AACGCACACA AAGGCTCGCT ATTTCCTTGA CTCATCTGAT 
GACGTTGTGA AACTTATTGG CAAGCTTTGC ACACACAACA ATGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPS4 ( NM_001341861.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001327998.1 Arabidopsis thaliana trehalose-6-phosphatase synthase S4 (TPS4), partial mRNA.

Price & Availability↑

CloneID	OAb39791
Clone ID Related Accession (Same CDS sequence)	NM_001341861.1
Accession Version	NM_001341861.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	trehalose-6-phosphatase synthase S4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). COMPLETENESS: incomplete on the 3' end.

ATGGCAAGGC CACGACTGCT TGTAGTGTCG ATGTCGCTTC CGGTCACTGC AAAGAGGACT 
GGAGAAGAGT CTTGGTCTTT CACGATGAGT CCTGGTGGTC TAGTTAGTGC TCTTCTAGGT 
TTGAAAGAAT TTGAGACCAA ATGGATTGGA TGGCCTGGAG TAGATGTTCA TGATGCGATT 
GGCAAAAAGA CACTCTCCAT TACCCTAGCT GAAAAGGGAT GTATCCCAGT GTTTTTGGAA 
GAAGTGTGTG ATCAATACTA TAATGGCTAC TGCAACAATA TTCTGTGGCC GATTTTTCAC 
TACTTGGGAA CGCCACCAGA ATATCGTAAC GATGCAACCA TAACATACCA GTCACAGTAT 
GAAGCATACA AGAAAGCAAA TCAAATTTTC TTTGATGTTG TCAAAGAGCA CTATGAAGAA 
GGAGATGTGG TTTGGTGCCA TGATTATCAT GTTATGCTTC TTCCTCAATA TCTTAAGGAA 
TACAACAGTA AGATGAAAGT TGGATGGTTT CTCCATACAC CATTTCCTTC TTCCGAGATG 
TACAAAACAT TGCCCTCGCG ATCCGATCTG CTTCGTTCTG TTCTTACTGC TGATTTAGTT 
GGTTTCCATA CCTATGACTT CGCAAGACAT TTTTTGAATG CATGCATGTG TATTCTTGGA 
GTCGAAGCAA CATCTGAAGG AATTGTGGAT CAGGGCAAAG TCACTCGAGT AGCTGTTTTT 
CCCATTGGGA TAGAACCTGA GAGGTTTATA AACACAAGTG AACTCTCTGA AGTTGTTCAA 
TACATGAAGA AGTTCAAAAA CGATTTTGGT GGGAGAAAGT TGATATTAGG TGTTGATCGT 
CTCGATACGA TCAAAGGGAT TCCACAAAAA TATCAGGCAT TTGAGAAGTT CTTAGAGGAA 
AATGCAGAGT GGCGTGGTAA AGTCATGTTA CTTCAGATTG CAGTGCCAAC AAGGAACGGT 
ATTGGTGAAT ATCAAAAGAT CAAAGACCAA TGTCATTACC ATGTTGGACG AATTAATGGT 
CGTTTTGGTT CCATCTCTTC TGTTCCAATA ATTCACCTGG ATTGTTCTAT TGACTTTAAT 
CAATTATGCG CACTTTATGC CATCACAGAT GTATTACTTG TAACATCTCT GAGAGATGGA 
ATGAACCTTG TGAGTTCTGA ATTCATTGCC TGCCAAAAAG CAGAAAAAGG GGTTCTCATT 
CTAAGCGAGT TTGCAGGTGC TGGACAGTCA CTTGGTGCCG GAGCTATTTT AGTGAATCCT 
TGGAACATTA AAGAAGTTTC TTCTGCCATT GGAGAAGCTC TAAATATGTC ACATGAAGAA 
AAGGAGAGAA AACATAAAAT CAATTTTCAG TATGTGAAAA CTCACTCCAC TCAGCAGTGG 
GCAGATGATT TCATGAATAA ACTTATTGAG ATTACAACCA GCGCGGAGCT AGGAGCTGGA 
TTAGCCGCCA CACTTGAACT TCCAGAACAT GATGTGATTC AACAATACTC AAAGTCTAAC 
AATAGACTGC TGATTCTTGG ATTCTATGGA ACGCTCACTC AGCCGATGAA AAATCAAGAG 
AGAAGAGGCG ACGGAATGAA TCTTGAACTG CATCCACAGC TAAAAGAGAG ACTCAAAGAA 
TTATGCAGTG ATCCAAAAAC AACAGTAGTT GTCCTGAGTA GAAGTGAAAA ATGCATATTG 
GATAAAAACT TTGGAGAATA TAACATGTGG TTGGCTGCTG AAAATGGGAT GTTTCTAAGG 
CATACAAGTG GAGAATGGGT GACAAGAATT CCAGAACACA TGAATTTGGA ATGGATTGAT 
GGTGTTAAGC ATGTTTTCAA GTACTTCACT GAAAGAACTC CAGGATCATA TCTCGAAACA 
AGCGAGGCTT CGCTTGTATG GAACTACGAA AATGCAGATG CTGAATTCGG GAGAGCTCAA 
GCAAGAGACA TGTTACAACA CTTGTGGGCA GGACCAATCT CTAATGCCTC AGTGGATGTT 
GTTAGAGGAG GCCAATCTGT TGAAGTCCAT GCCGTTGGTG TGACAAAGGG AAGTGCAATG 
GAGCGTATTC TCGGAGAAAT AGTGCATAAC AAGTCAATGG CTACACCAAT TGACTATGTC 
TTGTGCATTG GTTGTTTCTT GGGGAAAGAC GAAGACGTTT ACACATTCTT CGAGCCAGAG 
TTGACCAAGA AAGCCAAGTC GCTTTCTTCT TCAGGTAGTG ACTCCCCCAA GAAGGTATCA 
TCGACCATTG TTGACCTCAA GGGAGAGAAT TACTTCTCCG TAGCTATTGG ACAAACGCAC 
ACAAAGGCTC GCTATTTCCT TGACTCATCT GATGACGTTG TGAAACTTAT TGGCAAGCTT 
TGCACACACA ACAATGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001327998.1
CDS	41..2401
Translation	MARPRLLVVSMSLPVTAKRTGEESWSFTMSPGGLVSALLGLKEFETKWIGWPGVDVHDAIGKKTLSITLAEKGCIPVFLEEVCDQYYNGYCNNILWPIFHYLGTPPEYRNDATITYQSQYEAYKKANQIFFDVVKEHYEEGDVVWCHDYHVMLLPQYLKEYNSKMKVGWFLHTPFPSSEMYKTLPSRSDLLRSVLTADLVGFHTYDFARHFLNACMCILGVEATSEGIVDQGKVTRVAVFPIGIEPERFINTSELSEVVQYMKKFKNDFGGRKLILGVDRLDTIKGIPQKYQAFEKFLEENAEWRGKVMLLQIAVPTRNGIGEYQKIKDQCHYHVGRINGRFGSISSVPIIHLDCSIDFNQLCALYAITDVLLVTSLRDGMNLVSSEFIACQKAEKGVLILSEFAGAGQSLGAGAILVNPWNIKEVSSAIGEALNMSHEEKERKHKINFQYVKTHSTQQWADDFMNKLIEITTSAELGAGLAATLELPEHDVIQQYSKSNNRLLILGFYGTLTQPMKNQERRGDGMNLELHPQLKERLKELCSDPKTTVVVLSRSEKCILDKNFGEYNMWLAAENGMFLRHTSGEWVTRIPEHMNLEWIDGVKHVFKYFTERTPGSYLETSEASLVWNYENADAEFGRAQARDMLQHLWAGPISNASVDVVRGGQSVEVHAVGVTKGSAMERILGEIVHNKSMATPIDYVLCIGCFLGKDEDVYTFFEPELTKKAKSLSSSGSDSPKKVSSTIVDLKGENYFSVAIGQTHTKARYFLDSSDDVVKLIGKLCTHNNA

Target ORF information:

RefSeq Version	NM_001341861.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana trehalose-6-phosphatase synthase S4 (TPS4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001341861.1

ORF Insert Sequence:

ATGGCAAGGC CACGACTGCT TGTAGTGTCG ATGTCGCTTC CGGTCACTGC AAAGAGGACT 
GGAGAAGAGT CTTGGTCTTT CACGATGAGT CCTGGTGGTC TAGTTAGTGC TCTTCTAGGT 
TTGAAAGAAT TTGAGACCAA ATGGATTGGA TGGCCTGGAG TAGATGTTCA TGATGCGATT 
GGCAAAAAGA CACTCTCCAT TACCCTAGCT GAAAAGGGAT GTATCCCAGT GTTTTTGGAA 
GAAGTGTGTG ATCAATACTA TAATGGCTAC TGCAACAATA TTCTGTGGCC GATTTTTCAC 
TACTTGGGAA CGCCACCAGA ATATCGTAAC GATGCAACCA TAACATACCA GTCACAGTAT 
GAAGCATACA AGAAAGCAAA TCAAATTTTC TTTGATGTTG TCAAAGAGCA CTATGAAGAA 
GGAGATGTGG TTTGGTGCCA TGATTATCAT GTTATGCTTC TTCCTCAATA TCTTAAGGAA 
TACAACAGTA AGATGAAAGT TGGATGGTTT CTCCATACAC CATTTCCTTC TTCCGAGATG 
TACAAAACAT TGCCCTCGCG ATCCGATCTG CTTCGTTCTG TTCTTACTGC TGATTTAGTT 
GGTTTCCATA CCTATGACTT CGCAAGACAT TTTTTGAATG CATGCATGTG TATTCTTGGA 
GTCGAAGCAA CATCTGAAGG AATTGTGGAT CAGGGCAAAG TCACTCGAGT AGCTGTTTTT 
CCCATTGGGA TAGAACCTGA GAGGTTTATA AACACAAGTG AACTCTCTGA AGTTGTTCAA 
TACATGAAGA AGTTCAAAAA CGATTTTGGT GGGAGAAAGT TGATATTAGG TGTTGATCGT 
CTCGATACGA TCAAAGGGAT TCCACAAAAA TATCAGGCAT TTGAGAAGTT CTTAGAGGAA 
AATGCAGAGT GGCGTGGTAA AGTCATGTTA CTTCAGATTG CAGTGCCAAC AAGGAACGGT 
ATTGGTGAAT ATCAAAAGAT CAAAGACCAA TGTCATTACC ATGTTGGACG AATTAATGGT 
CGTTTTGGTT CCATCTCTTC TGTTCCAATA ATTCACCTGG ATTGTTCTAT TGACTTTAAT 
CAATTATGCG CACTTTATGC CATCACAGAT GTATTACTTG TAACATCTCT GAGAGATGGA 
ATGAACCTTG TGAGTTCTGA ATTCATTGCC TGCCAAAAAG CAGAAAAAGG GGTTCTCATT 
CTAAGCGAGT TTGCAGGTGC TGGACAGTCA CTTGGTGCCG GAGCTATTTT AGTGAATCCT 
TGGAACATTA AAGAAGTTTC TTCTGCCATT GGAGAAGCTC TAAATATGTC ACATGAAGAA 
AAGGAGAGAA AACATAAAAT CAATTTTCAG TATGTGAAAA CTCACTCCAC TCAGCAGTGG 
GCAGATGATT TCATGAATAA ACTTATTGAG ATTACAACCA GCGCGGAGCT AGGAGCTGGA 
TTAGCCGCCA CACTTGAACT TCCAGAACAT GATGTGATTC AACAATACTC AAAGTCTAAC 
AATAGACTGC TGATTCTTGG ATTCTATGGA ACGCTCACTC AGCCGATGAA AAATCAAGAG 
AGAAGAGGCG ACGGAATGAA TCTTGAACTG CATCCACAGC TAAAAGAGAG ACTCAAAGAA 
TTATGCAGTG ATCCAAAAAC AACAGTAGTT GTCCTGAGTA GAAGTGAAAA ATGCATATTG 
GATAAAAACT TTGGAGAATA TAACATGTGG TTGGCTGCTG AAAATGGGAT GTTTCTAAGG 
CATACAAGTG GAGAATGGGT GACAAGAATT CCAGAACACA TGAATTTGGA ATGGATTGAT 
GGTGTTAAGC ATGTTTTCAA GTACTTCACT GAAAGAACTC CAGGATCATA TCTCGAAACA 
AGCGAGGCTT CGCTTGTATG GAACTACGAA AATGCAGATG CTGAATTCGG GAGAGCTCAA 
GCAAGAGACA TGTTACAACA CTTGTGGGCA GGACCAATCT CTAATGCCTC AGTGGATGTT 
GTTAGAGGAG GCCAATCTGT TGAAGTCCAT GCCGTTGGTG TGACAAAGGG AAGTGCAATG 
GAGCGTATTC TCGGAGAAAT AGTGCATAAC AAGTCAATGG CTACACCAAT TGACTATGTC 
TTGTGCATTG GTTGTTTCTT GGGGAAAGAC GAAGACGTTT ACACATTCTT CGAGCCAGAG 
TTGACCAAGA AAGCCAAGTC GCTTTCTTCT TCAGGTAGTG ACTCCCCCAA GAAGGTATCA 
TCGACCATTG TTGACCTCAA GGGAGAGAAT TACTTCTCCG TAGCTATTGG ACAAACGCAC 
ACAAAGGCTC GCTATTTCCT TGACTCATCT GATGACGTTG TGAAACTTAT TGGCAAGCTT 
TGCACACACA ACAATGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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TPS4 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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