NLP7 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	NLP7
Entrez Gene ID	828502
Full Name	NIN like protein 7
Synonyms	NIN like protein 7,T19F6.10,T19F6_10
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_118534.6	NP_194133.1	NIN like protein 7
NM_001341638.1	NP_001328405.1	NIN like protein 7

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	NLP7	NP_194133.1
Oryza sativa (rice)	Os01g0236700	NP_001042530.1
Arabidopsis thaliana (thale cress)	AT1G64530	NP_176634.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

nitrate-coupled CPK signalling phosphorylates conserved NIN-LIKE PROTEIN (NLP) transcription factors to specify the reprogramming of gene sets for downstream transcription factors, transporters, nitrogen assimilation, carbon/nitrogen metabolism, redox, signalling, hormones and proliferation
Title: Discovery of nitrate-CPK-NLP signalling in central nutrient-growth networks.

NLP7 is an important element of the nitrate signal transduction pathway and is proposed as a new regulatory protein specific for nitrogen assimilation in non-nodulating plants. [NLP7]
Title: The nodule inception-like protein 7 modulates nitrate sensing and metabolism in Arabidopsis.

The following NLP7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NLP7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb16119	NM_118534.6 Latest version!	Arabidopsis thaliana NIN like protein 7 (NLP7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb39647	NM_001341638.1 Latest version!	Arabidopsis thaliana NIN like protein 7 (NLP7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NLP7 ( NM_118534.6 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_194133.1 Arabidopsis thaliana NIN like protein 7 (NLP7), mRNA.

Price & Availability↑

CloneID	OAb16119
Clone ID Related Accession (Same CDS sequence)	NM_118534.6
Accession Version	NM_118534.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	NIN like protein 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_118534.5.

ATGTGCGAGC CCGATGATAA TTCCGCTAGA AACGGCGTCA CTACTCAACC TTCGAGGTCA 
AGGGAGCTTC TAATGGATGT TGACGACTTA GATCTTGACG GTTCATGGCC ACTAGATCAA 
ATCCCTTACT TATCCTCATC GAATCGCATG ATTTCTCCGA TTTTTGTCTC CTCTTCCTCT 
GAGCAGCCTT GCTCGCCTCT CTGGGCTTTC TCCGACGGTG GAGGAAATGG TTTTCACCAC 
GCAACCTCCG GTGGCGATGA TGAGAAGATC AGCTCTGTCT CCGGTGTTCC TTCTTTCCGT 
CTCGCCGAGT ATCCTCTCTT CCTCCCTTAC TCTTCTCCAT CAGCAGCTGA GAACACAACA 
GAGAAGCATA ACAGTTTCCA GTTTCCGTCT CCATTGATGA GCCTAGTCCC ACCAGAGAAC 
ACAGACAACT ACTGTGTGAT CAAAGAGAGG ATGACTCAGG CGCTTCGATA CTTCAAAGAA 
TCAACCGAAC AACACGTTTT GGCTCAGGTC TGGGCTCCTG TGAGAAAGAA TGGTCGTGAT 
TTGCTGACGA CTTTGGGTCA ACCTTTTGTT CTTAATCCTA ATGGTAATGG GCTTAATCAA 
TACAGGATGA TCTCTCTCAC ATATATGTTT TCTGTGGATA GTGAAAGTGA CGTAGAGCTC 
GGACTCCCGG GTCGAGTTTT CCGTCAGAAA TTGCCTGAAT GGACTCCAAA TGTTCAGTAC 
TATTCCAGCA AAGAATTCTC GCGGCTTGAT CACGCCTTGC ACTACAACGT GCGTGGTACA 
CTGGCCTTGC CTGTCTTTAA TCCCTCTGGT CAGTCCTGCA TAGGTGTTGT GGAACTTATA 
ATGACCTCAG AGAAGATTCA CTATGCACCC GAAGTGGACA AAGTTTGCAA AGCCCTTGAG 
GCGGTAAATC TGAAAAGCTC GGAAATACTT GATCACCAAA CAACACAGAT ATGCAATGAG 
AGTCGCCAAA ACGCGCTTGC TGAGATTCTC GAAGTGCTGA CAGTTGTATG TGAGACCCAT 
AACTTGCCTC TCGCTCAGAC TTGGGTTCCA TGTCAGCATG GGAGCGTTCT TGCCAATGGT 
GGCGGTCTAA AGAAAAACTG CACCAGCTTT GACGGTAGCT GCATGGGTCA AATCTGCATG 
TCTACAACCG ACATGGCCTG CTATGTCGTG GATGCTCATG TCTGGGGCTT TAGAGATGCC 
TGTCTTGAAC ACCATCTCCA GAAAGGCCAG GGAGTCGCTG GACGAGCTTT TCTCAATGGT 
GGCTCATGTT TCTGCAGAGA CATCACCAAG TTCTGCAAAA CGCAGTACCC ACTAGTCCAT 
TATGCGCTCA TGTTCAAGTT GACCACTTGT TTTGCAATAT CTCTCCAGAG CTCTTACACG 
GGCGACGACA GTTACATTCT TGAATTTTTT CTTCCTTCGA GTATAACAGA CGACCAAGAG 
CAAGATTTGC TGTTGGGTTC TATTTTGGTG ACAATGAAAG AACATTTTCA GAGTCTGAGG 
GTTGCATCTG GGGTTGACTT TGGTGAAGAT GACGACAAAT TGTCTTTCGA GATCATCCAA 
GCATTACCGG ACAAGAAGGT TCATTCAAAA ATAGAATCCA TTCGAGTTCC CTTTTCTGGT 
TTTAAGTCAA ATGCAACAGA GACGATGTTG ATTCCTCAGC CTGTGGTTCA GTCTTCTGAT 
CCAGTAAATG AGAAAATCAA CGTGGCCACT GTTAACGGTG TGGTTAAGGA GAAGAAGAAA 
ACAGAGAAAA AGCGTGGGAA GACTGAGAAA ACAATCAGTC TAGATGTACT TCAGCAGTAT 
TTCACTGGAA GTCTCAAAGA CGCTGCAAAG AGCCTAGGAG TTTGCCCGAC GACAATGAAG 
CGAATTTGCA GGCAACACGG AATCTCGCGG TGGCCATCGA GGAAGATCAA GAAAGTGAAT 
CGTTCAATCA CAAAGCTGAA ACGAGTCATC GAATCTGTTC AAGGTACTGA TGGAGGCCTC 
GACCTGACTT CCATGGCCGT TAGTTCCATC CCTTGGACAC ACGGTCAAAC ATCAGCACAG 
CCACTAAACT CACCCAATGG TTCCAAACCA CCTGAGCTAC CAAACACCAA TAATTCACCT 
AACCATTGGT CAAGTGATCA CAGTCCGAAC GAGCCAAATG GTTCGCCTGA GTTACCACCA 
AGCAATGGTC ACAAGCGATC ACGAACGGTG GATGAGAGCG CTGGGACTCC AACCTCTCAT 
GGCTCATGTG ACGGTAACCA ATTAGATGAA CCGAAAGTCC CAAATCAAGA TCCGCTCTTC 
ACGGTTGGTG GATCACCCGG GCTCCTTTTT CCACCTTATT CTAGAGATCA TGATGTATCT 
GCAGCTTCCT TCGCAATGCC GAACAGGCTT CTTGGTTCTA TAGACCATTT CCGAGGAATG 
CTCATTGAAG ACGCTGGAAG TTCAAAAGAT CTGAGAAATC TCTGCCCCAC TGCAGCATTT 
GACGATAAGT TTCAAGACAC AAACTGGATG AACAATGATA ATAATAGCAA CAACAACTTA 
TACGCTCCCC CAAAGGAAGA GGCCATTGCA AATGTTGCAT GCGAACCATC AGGCTCAGAA 
ATGAGAACGG TAACAATCAA AGCAAGTTAC AAAGACGACA TAATACGGTT CAGAATATCC 
TCGGGTTCAG GTATAATGGA ATTGAAGGAT GAAGTGGCTA AGAGGCTGAA AGTTGATGCA 
GGAACGTTCG ATATCAAGTA TCTTGACGAT GATAACGAAT GGGTTTTAAT AGCTTGTGAT 
GCTGATCTTC AAGAATGTCT CGAGATCCCT AGATCCTCCC GCACGAAAAT CGTAAGGCTC 
TTAGTTCATG ATGTAACGAC AAATCTAGGG AGCTCCTGCG AGAGCACTGG AGAATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_194133.1
CDS	377..3256
Translation	MCEPDDNSARNGVTTQPSRSRELLMDVDDLDLDGSWPLDQIPYLSSSNRMISPIFVSSSSEQPCSPLWAFSDGGGNGFHHATSGGDDEKISSVSGVPSFRLAEYPLFLPYSSPSAAENTTEKHNSFQFPSPLMSLVPPENTDNYCVIKERMTQALRYFKESTEQHVLAQVWAPVRKNGRDLLTTLGQPFVLNPNGNGLNQYRMISLTYMFSVDSESDVELGLPGRVFRQKLPEWTPNVQYYSSKEFSRLDHALHYNVRGTLALPVFNPSGQSCIGVVELIMTSEKIHYAPEVDKVCKALEAVNLKSSEILDHQTTQICNESRQNALAEILEVLTVVCETHNLPLAQTWVPCQHGSVLANGGGLKKNCTSFDGSCMGQICMSTTDMACYVVDAHVWGFRDACLEHHLQKGQGVAGRAFLNGGSCFCRDITKFCKTQYPLVHYALMFKLTTCFAISLQSSYTGDDSYILEFFLPSSITDDQEQDLLLGSILVTMKEHFQSLRVASGVDFGEDDDKLSFEIIQALPDKKVHSKIESIRVPFSGFKSNATETMLIPQPVVQSSDPVNEKINVATVNGVVKEKKKTEKKRGKTEKTISLDVLQQYFTGSLKDAAKSLGVCPTTMKRICRQHGISRWPSRKIKKVNRSITKLKRVIESVQGTDGGLDLTSMAVSSIPWTHGQTSAQPLNSPNGSKPPELPNTNNSPNHWSSDHSPNEPNGSPELPPSNGHKRSRTVDESAGTPTSHGSCDGNQLDEPKVPNQDPLFTVGGSPGLLFPPYSRDHDVSAASFAMPNRLLGSIDHFRGMLIEDAGSSKDLRNLCPTAAFDDKFQDTNWMNNDNNSNNNLYAPPKEEAIANVACEPSGSEMRTVTIKASYKDDIIRFRISSGSGIMELKDEVAKRLKVDAGTFDIKYLDDDNEWVLIACDADLQECLEIPRSSRTKIVRLLVHDVTTNLGSSCESTGEL

Target ORF information:

RefSeq Version	NM_118534.6
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana NIN like protein 7 (NLP7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_118534.6

ORF Insert Sequence:

ATGTGCGAGC CCGATGATAA TTCCGCTAGA AACGGCGTCA CTACTCAACC TTCGAGGTCA 
AGGGAGCTTC TAATGGATGT TGACGACTTA GATCTTGACG GTTCATGGCC ACTAGATCAA 
ATCCCTTACT TATCCTCATC GAATCGCATG ATTTCTCCGA TTTTTGTCTC CTCTTCCTCT 
GAGCAGCCTT GCTCGCCTCT CTGGGCTTTC TCCGACGGTG GAGGAAATGG TTTTCACCAC 
GCAACCTCCG GTGGCGATGA TGAGAAGATC AGCTCTGTCT CCGGTGTTCC TTCTTTCCGT 
CTCGCCGAGT ATCCTCTCTT CCTCCCTTAC TCTTCTCCAT CAGCAGCTGA GAACACAACA 
GAGAAGCATA ACAGTTTCCA GTTTCCGTCT CCATTGATGA GCCTAGTCCC ACCAGAGAAC 
ACAGACAACT ACTGTGTGAT CAAAGAGAGG ATGACTCAGG CGCTTCGATA CTTCAAAGAA 
TCAACCGAAC AACACGTTTT GGCTCAGGTC TGGGCTCCTG TGAGAAAGAA TGGTCGTGAT 
TTGCTGACGA CTTTGGGTCA ACCTTTTGTT CTTAATCCTA ATGGTAATGG GCTTAATCAA 
TACAGGATGA TCTCTCTCAC ATATATGTTT TCTGTGGATA GTGAAAGTGA CGTAGAGCTC 
GGACTCCCGG GTCGAGTTTT CCGTCAGAAA TTGCCTGAAT GGACTCCAAA TGTTCAGTAC 
TATTCCAGCA AAGAATTCTC GCGGCTTGAT CACGCCTTGC ACTACAACGT GCGTGGTACA 
CTGGCCTTGC CTGTCTTTAA TCCCTCTGGT CAGTCCTGCA TAGGTGTTGT GGAACTTATA 
ATGACCTCAG AGAAGATTCA CTATGCACCC GAAGTGGACA AAGTTTGCAA AGCCCTTGAG 
GCGGTAAATC TGAAAAGCTC GGAAATACTT GATCACCAAA CAACACAGAT ATGCAATGAG 
AGTCGCCAAA ACGCGCTTGC TGAGATTCTC GAAGTGCTGA CAGTTGTATG TGAGACCCAT 
AACTTGCCTC TCGCTCAGAC TTGGGTTCCA TGTCAGCATG GGAGCGTTCT TGCCAATGGT 
GGCGGTCTAA AGAAAAACTG CACCAGCTTT GACGGTAGCT GCATGGGTCA AATCTGCATG 
TCTACAACCG ACATGGCCTG CTATGTCGTG GATGCTCATG TCTGGGGCTT TAGAGATGCC 
TGTCTTGAAC ACCATCTCCA GAAAGGCCAG GGAGTCGCTG GACGAGCTTT TCTCAATGGT 
GGCTCATGTT TCTGCAGAGA CATCACCAAG TTCTGCAAAA CGCAGTACCC ACTAGTCCAT 
TATGCGCTCA TGTTCAAGTT GACCACTTGT TTTGCAATAT CTCTCCAGAG CTCTTACACG 
GGCGACGACA GTTACATTCT TGAATTTTTT CTTCCTTCGA GTATAACAGA CGACCAAGAG 
CAAGATTTGC TGTTGGGTTC TATTTTGGTG ACAATGAAAG AACATTTTCA GAGTCTGAGG 
GTTGCATCTG GGGTTGACTT TGGTGAAGAT GACGACAAAT TGTCTTTCGA GATCATCCAA 
GCATTACCGG ACAAGAAGGT TCATTCAAAA ATAGAATCCA TTCGAGTTCC CTTTTCTGGT 
TTTAAGTCAA ATGCAACAGA GACGATGTTG ATTCCTCAGC CTGTGGTTCA GTCTTCTGAT 
CCAGTAAATG AGAAAATCAA CGTGGCCACT GTTAACGGTG TGGTTAAGGA GAAGAAGAAA 
ACAGAGAAAA AGCGTGGGAA GACTGAGAAA ACAATCAGTC TAGATGTACT TCAGCAGTAT 
TTCACTGGAA GTCTCAAAGA CGCTGCAAAG AGCCTAGGAG TTTGCCCGAC GACAATGAAG 
CGAATTTGCA GGCAACACGG AATCTCGCGG TGGCCATCGA GGAAGATCAA GAAAGTGAAT 
CGTTCAATCA CAAAGCTGAA ACGAGTCATC GAATCTGTTC AAGGTACTGA TGGAGGCCTC 
GACCTGACTT CCATGGCCGT TAGTTCCATC CCTTGGACAC ACGGTCAAAC ATCAGCACAG 
CCACTAAACT CACCCAATGG TTCCAAACCA CCTGAGCTAC CAAACACCAA TAATTCACCT 
AACCATTGGT CAAGTGATCA CAGTCCGAAC GAGCCAAATG GTTCGCCTGA GTTACCACCA 
AGCAATGGTC ACAAGCGATC ACGAACGGTG GATGAGAGCG CTGGGACTCC AACCTCTCAT 
GGCTCATGTG ACGGTAACCA ATTAGATGAA CCGAAAGTCC CAAATCAAGA TCCGCTCTTC 
ACGGTTGGTG GATCACCCGG GCTCCTTTTT CCACCTTATT CTAGAGATCA TGATGTATCT 
GCAGCTTCCT TCGCAATGCC GAACAGGCTT CTTGGTTCTA TAGACCATTT CCGAGGAATG 
CTCATTGAAG ACGCTGGAAG TTCAAAAGAT CTGAGAAATC TCTGCCCCAC TGCAGCATTT 
GACGATAAGT TTCAAGACAC AAACTGGATG AACAATGATA ATAATAGCAA CAACAACTTA 
TACGCTCCCC CAAAGGAAGA GGCCATTGCA AATGTTGCAT GCGAACCATC AGGCTCAGAA 
ATGAGAACGG TAACAATCAA AGCAAGTTAC AAAGACGACA TAATACGGTT CAGAATATCC 
TCGGGTTCAG GTATAATGGA ATTGAAGGAT GAAGTGGCTA AGAGGCTGAA AGTTGATGCA 
GGAACGTTCG ATATCAAGTA TCTTGACGAT GATAACGAAT GGGTTTTAAT AGCTTGTGAT 
GCTGATCTTC AAGAATGTCT CGAGATCCCT AGATCCTCCC GCACGAAAAT CGTAAGGCTC 
TTAGTTCATG ATGTAACGAC AAATCTAGGG AGCTCCTGCG AGAGCACTGG AGAATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLP7 ( NM_001341638.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001328405.1 Arabidopsis thaliana NIN like protein 7 (NLP7), mRNA.

Price & Availability↑

CloneID	OAb39647
Clone ID Related Accession (Same CDS sequence)	NM_001341638.1
Accession Version	NM_001341638.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	NIN like protein 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGACCTCAG AGAAGATTCA CTATGCACCC GAAGTGGACA AAGTTTGCAA AGCCCTTGAG 
GCGGTAAATC TGAAAAGCTC GGAAATACTT GATCACCAAA CAACACAGAT ATGCAATGAG 
AGTCGCCAAA ACGCGCTTGC TGAGATTCTC GAAGTGCTGA CAGTTGTATG TGAGACCCAT 
AACTTGCCTC TCGCTCAGAC TTGGGTTCCA TGTCAGCATG GGAGCGTTCT TGCCAATGGT 
GGCGGTCTAA AGAAAAACTG CACCAGCTTT GACGGTAGCT GCATGGGTCA AATCTGCATG 
TCTACAACCG ACATGGCCTG CTATGTCGTG GATGCTCATG TCTGGGGCTT TAGAGATGCC 
TGTCTTGAAC ACCATCTCCA GAAAGGCCAG GGAGTCGCTG GACGAGCTTT TCTCAATGGT 
GGCTCATGTT TCTGCAGAGA CATCACCAAG TTCTGCAAAA CGCAGTACCC ACTAGTCCAT 
TATGCGCTCA TGTTCAAGTT GACCACTTGT TTTGCAATAT CTCTCCAGAG CTCTTACACG 
GGCGACGACA GTTACATTCT TGAATTTTTT CTTCCTTCGA GTATAACAGA CGACCAAGAG 
CAAGATTTGC TGTTGGGTTC TATTTTGGTG ACAATGAAAG AACATTTTCA GAGTCTGAGG 
GTTGCATCTG GGGTTGACTT TGGTGAAGAT GACGACAAAT TGTCTTTCGA GATCATCCAA 
GCATTACCGG ACAAGAAGGT TCATTCAAAA ATAGAATCCA TTCGAGTTCC CTTTTCTGGT 
TTTAAGTCAA ATGCAACAGA GACGATGTTG ATTCCTCAGC CTGTGGTTCA GTCTTCTGAT 
CCAGTAAATG AGAAAATCAA CGTGGCCACT GTTAACGGTG TGGTTAAGGA GAAGAAGAAA 
ACAGAGAAAA AGCGTGGGAA GACTGAGAAA ACAATCAGTC TAGATGTACT TCAGCAGTAT 
TTCACTGGAA GTCTCAAAGA CGCTGCAAAG AGCCTAGGAG TTTGCCCGAC GACAATGAAG 
CGAATTTGCA GGCAACACGG AATCTCGCGG TGGCCATCGA GGAAGATCAA GAAAGTGAAT 
CGTTCAATCA CAAAGCTGAA ACGAGTCATC GAATCTGTTC AAGGTACTGA TGGAGGCCTC 
GACCTGACTT CCATGGCCGT TAGTTCCATC CCTTGGACAC ACGGTCAAAC ATCAGCACAG 
CCACTAAACT CACCCAATGG TTCCAAACCA CCTGAGCTAC CAAACACCAA TAATTCACCT 
AACCATTGGT CAAGTGATCA CAGTCCGAAC GAGCCAAATG GTTCGCCTGA GTTACCACCA 
AGCAATGGTC ACAAGCGATC ACGAACGGTG GATGAGAGCG CTGGGACTCC AACCTCTCAT 
GGCTCATGTG ACGGTAACCA ATTAGATGAA CCGAAAGTCC CAAATCAAGA TCCGCTCTTC 
ACGGTTGGTG GATCACCCGG GCTCCTTTTT CCACCTTATT CTAGAGATCA TGATGTATCT 
GCAGCTTCCT TCGCAATGCC GAACAGGCTT CTTGGTTCTA TAGACCATTT CCGAGGAATG 
CTCATTGAAG ACGCTGGAAG TTCAAAAGAT CTGAGAAATC TCTGCCCCAC TGCAGCATTT 
GACGATAAGT TTCAAGACAC AAACTGGATG AACAATGATA ATAATAGCAA CAACAACTTA 
TACGCTCCCC CAAAGGAAGA GGCCATTGCA AATGTTGCAT GCGAACCATC AGGCTCAGAA 
ATGAGAACGG TAACAATCAA AGCAAGTTAC AAAGACGACA TAATACGGTT CAGAATATCC 
TCGGGTTCAG GTATAATGGA ATTGAAGGAT GAAGTGGCTA AGAGGCTGAA AGTTGATGCA 
GGAACGTTCG ATATCAAGTA TCTTGACGAT GATAACGAAT GGGTTTTAAT AGCTTGTGAT 
GCTGATCTTC AAGAATGTCT CGAGATCCCT AGATCCTCCC GCACGAAAAT CGTAAGGCTC 
TTAGTTCATG ATGTAACGAC AAATCTAGGG AGCTCCTGCG AGAGCACTGG AGAATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001328405.1
CDS	1083..3122
Translation	MTSEKIHYAPEVDKVCKALEAVNLKSSEILDHQTTQICNESRQNALAEILEVLTVVCETHNLPLAQTWVPCQHGSVLANGGGLKKNCTSFDGSCMGQICMSTTDMACYVVDAHVWGFRDACLEHHLQKGQGVAGRAFLNGGSCFCRDITKFCKTQYPLVHYALMFKLTTCFAISLQSSYTGDDSYILEFFLPSSITDDQEQDLLLGSILVTMKEHFQSLRVASGVDFGEDDDKLSFEIIQALPDKKVHSKIESIRVPFSGFKSNATETMLIPQPVVQSSDPVNEKINVATVNGVVKEKKKTEKKRGKTEKTISLDVLQQYFTGSLKDAAKSLGVCPTTMKRICRQHGISRWPSRKIKKVNRSITKLKRVIESVQGTDGGLDLTSMAVSSIPWTHGQTSAQPLNSPNGSKPPELPNTNNSPNHWSSDHSPNEPNGSPELPPSNGHKRSRTVDESAGTPTSHGSCDGNQLDEPKVPNQDPLFTVGGSPGLLFPPYSRDHDVSAASFAMPNRLLGSIDHFRGMLIEDAGSSKDLRNLCPTAAFDDKFQDTNWMNNDNNSNNNLYAPPKEEAIANVACEPSGSEMRTVTIKASYKDDIIRFRISSGSGIMELKDEVAKRLKVDAGTFDIKYLDDDNEWVLIACDADLQECLEIPRSSRTKIVRLLVHDVTTNLGSSCESTGEL

Target ORF information:

RefSeq Version	NM_001341638.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana NIN like protein 7 (NLP7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001341638.1

ORF Insert Sequence:

ATGACCTCAG AGAAGATTCA CTATGCACCC GAAGTGGACA AAGTTTGCAA AGCCCTTGAG 
GCGGTAAATC TGAAAAGCTC GGAAATACTT GATCACCAAA CAACACAGAT ATGCAATGAG 
AGTCGCCAAA ACGCGCTTGC TGAGATTCTC GAAGTGCTGA CAGTTGTATG TGAGACCCAT 
AACTTGCCTC TCGCTCAGAC TTGGGTTCCA TGTCAGCATG GGAGCGTTCT TGCCAATGGT 
GGCGGTCTAA AGAAAAACTG CACCAGCTTT GACGGTAGCT GCATGGGTCA AATCTGCATG 
TCTACAACCG ACATGGCCTG CTATGTCGTG GATGCTCATG TCTGGGGCTT TAGAGATGCC 
TGTCTTGAAC ACCATCTCCA GAAAGGCCAG GGAGTCGCTG GACGAGCTTT TCTCAATGGT 
GGCTCATGTT TCTGCAGAGA CATCACCAAG TTCTGCAAAA CGCAGTACCC ACTAGTCCAT 
TATGCGCTCA TGTTCAAGTT GACCACTTGT TTTGCAATAT CTCTCCAGAG CTCTTACACG 
GGCGACGACA GTTACATTCT TGAATTTTTT CTTCCTTCGA GTATAACAGA CGACCAAGAG 
CAAGATTTGC TGTTGGGTTC TATTTTGGTG ACAATGAAAG AACATTTTCA GAGTCTGAGG 
GTTGCATCTG GGGTTGACTT TGGTGAAGAT GACGACAAAT TGTCTTTCGA GATCATCCAA 
GCATTACCGG ACAAGAAGGT TCATTCAAAA ATAGAATCCA TTCGAGTTCC CTTTTCTGGT 
TTTAAGTCAA ATGCAACAGA GACGATGTTG ATTCCTCAGC CTGTGGTTCA GTCTTCTGAT 
CCAGTAAATG AGAAAATCAA CGTGGCCACT GTTAACGGTG TGGTTAAGGA GAAGAAGAAA 
ACAGAGAAAA AGCGTGGGAA GACTGAGAAA ACAATCAGTC TAGATGTACT TCAGCAGTAT 
TTCACTGGAA GTCTCAAAGA CGCTGCAAAG AGCCTAGGAG TTTGCCCGAC GACAATGAAG 
CGAATTTGCA GGCAACACGG AATCTCGCGG TGGCCATCGA GGAAGATCAA GAAAGTGAAT 
CGTTCAATCA CAAAGCTGAA ACGAGTCATC GAATCTGTTC AAGGTACTGA TGGAGGCCTC 
GACCTGACTT CCATGGCCGT TAGTTCCATC CCTTGGACAC ACGGTCAAAC ATCAGCACAG 
CCACTAAACT CACCCAATGG TTCCAAACCA CCTGAGCTAC CAAACACCAA TAATTCACCT 
AACCATTGGT CAAGTGATCA CAGTCCGAAC GAGCCAAATG GTTCGCCTGA GTTACCACCA 
AGCAATGGTC ACAAGCGATC ACGAACGGTG GATGAGAGCG CTGGGACTCC AACCTCTCAT 
GGCTCATGTG ACGGTAACCA ATTAGATGAA CCGAAAGTCC CAAATCAAGA TCCGCTCTTC 
ACGGTTGGTG GATCACCCGG GCTCCTTTTT CCACCTTATT CTAGAGATCA TGATGTATCT 
GCAGCTTCCT TCGCAATGCC GAACAGGCTT CTTGGTTCTA TAGACCATTT CCGAGGAATG 
CTCATTGAAG ACGCTGGAAG TTCAAAAGAT CTGAGAAATC TCTGCCCCAC TGCAGCATTT 
GACGATAAGT TTCAAGACAC AAACTGGATG AACAATGATA ATAATAGCAA CAACAACTTA 
TACGCTCCCC CAAAGGAAGA GGCCATTGCA AATGTTGCAT GCGAACCATC AGGCTCAGAA 
ATGAGAACGG TAACAATCAA AGCAAGTTAC AAAGACGACA TAATACGGTT CAGAATATCC 
TCGGGTTCAG GTATAATGGA ATTGAAGGAT GAAGTGGCTA AGAGGCTGAA AGTTGATGCA 
GGAACGTTCG ATATCAAGTA TCTTGACGAT GATAACGAAT GGGTTTTAAT AGCTTGTGAT 
GCTGATCTTC AAGAATGTCT CGAGATCCCT AGATCCTCCC GCACGAAAAT CGTAAGGCTC 
TTAGTTCATG ATGTAACGAC AAATCTAGGG AGCTCCTGCG AGAGCACTGG AGAATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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NLP7 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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