VPS54 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS54
Entrez Gene ID	827690
Full Name	VPS54
Synonyms	ARABIDOPSIS THALIANA VPS54 HOMOLOG,ATVPS54,F24J7.50,F24J7_50
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_179078.3	NP_849409.2	VPS54
NM_118069.5	NP_193684.4	VPS54

Pathways from BioSystems

Homologies

Homo sapiens (human)	VPS54	NP_057600.2
Canis lupus familiaris (dog)	VPS54	XP_865972.2
	VPS54	XP_001087667.1
Bos taurus (cattle)	VPS54	NP_001098930.1
Mus musculus (house mouse)	Vps54	NP_620692.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008353	XP_317102.3
Pan troglodytes (chimpanzee)	VPS54	XP_515511.2
Rattus norvegicus (Norway rat)	Vps54	NP_775170.1
Gallus gallus (chicken)	VPS54	XP_419349.3
Oryza sativa (rice)	Os04g0212100	NP_001173796.1
Caenorhabditis elegans (roundworm)	vps-54	NP_001256226.1
Xenopus tropicalis (tropical clawed frog)	vps54	XP_004914765.1
Danio rerio (zebrafish)	vps54	NP_001124248.1
Drosophila melanogaster (fruit fly)	scat	NP_524905.1
Arabidopsis thaliana (thale cress)	VPS54	NP_849409.2

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following VPS54 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS54 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb15393	NM_118069.5 Latest version!	Arabidopsis thaliana VPS54 (VPS54), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OAb15393	NM_179078.3 Latest version!	Arabidopsis thaliana VPS54 (VPS54), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS54 ( NM_179078.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_849409.2 Arabidopsis thaliana VPS54 (VPS54), mRNA.

Price & Availability↑

CloneID	OAb15393
Clone ID Related Accession (Same CDS sequence)	NM_179078.3 , NM_118069.5
Accession Version	NM_179078.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	VPS54
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_179078.2.

ATGGATTCAC ACCCTTCCCT AATGGGAAGG TCCATTACCA ATTCGAACCG ATCGAGCTTA 
GATCTTGGTC GTCCTTCTTC GTCTTCTTCA TCTTCTCCTT CACCGTTAAC CAAATCGATC 
TCCGATGCAA GTAGTCAAAG TCTCTCTTCG ATCCTTAACA ATCCACACGG AGGTAAGTCT 
GGTGTTTATG GTTCCGATGC ATCTTGGGTT GGTTGGTGGT CTTCATCGAC ATTTGTAGCT 
CCAGCTGAGT TTGCGCCTGT TGCTTCAACG AAGCTCCCTG GTTCGGAGCT AACCAGATCC 
GACTTCCATG GCTACGTTTC TTCGATCTCT GAGTCTCATG GTAGATTTGA GGATATTAGG 
AAGCATACTA GGGAAGAGAG CTGTGGGTTT GATCAGGAGA GCCATGTATC GGGTTTAGCT 
GCGTGTTTGC GTGAGGTTCC TTCGCTTTAC TTTAAGGAGG ATTTTGCTCT TGAGGACGGA 
GCTACATTTC GTTCTGCTTG CCCTTTCTCG AGCCTTAATG AGAATCTAGC GCTTCAAGAG 
AAGTTGTCAC AGTATCTTGA TGTGGTGGAG CTGCATCTTG TGAAGGAGAT CTCTGTGAGG 
TCTGATTCGT TTTTTGAGGC TCAAGGACAG TTACAGGATT TGAATGTGAA GATTGTTGAG 
GGTTGTAGTA GGATTCGTGA GTTGAAAGAG ACTATTCGGC TCCTGGATAG GAATTTGGTA 
GATTCAGCTC GACAGATTCA AGAGCTGAGC TCAACTAGGA TTAATATGCT GGAGCTGCAA 
CGTAAATTAA GACTTATTTT GTATGTTAAC CAAGCTCTCT CTGCACTCAA ATTGCTTGTT 
GCGTCCGCAG ATTGTGCTGG AGCTTTGGAT ATAACTGACG ATCTCCAGAA CTTGCTGGCT 
GGGGATGAGC TTACTGGTCT ATACTGCTTC CGCCATCTTC GGGATCATGT TACTAGTTCC 
ATAGATTCCA TAAACAGTAT TCTCACTTCA GAATTCATGC GTATCTCCAT ACATGATACT 
GGGGAGATAG ATGTACTGAT TTTATCTGCA GCAAATATAC GGGGATCCAT TTCATCTAAC 
GGGAATACCG GCGAAGAAGT TAAGTTAGAA GAAGAGGATA CCTCTACCTT ATGTGATCGG 
CTTCTTCCTT TAGTTATTGG ATTGCTAAGA ACGGCAAAGT TCCCGTCAAT TTTGAGGATG 
TATCGTGATA CACTCACATC CGAAATGAAA AATGCAATCA AGAAAGCAGT TGCTGATCTG 
CTTCCCATTC TTGTTGCAAG ATCGCTCGAA TCTGATTTTT CACATGGGGA GCGCTCTGTA 
GATGATGGTG GTGGCTTATC TCTCGCGAGC AAATTGAGGA CTCTGTCATC TGAGGCTTTT 
GTTAATCTCT TAACTGCTAT ATTTAAGATT GTTCAGGCAC ATCTAGTCCG GGCTTCGGAA 
GTGAAAAAAG CAATTGAATG GATTCTCTGC AATATCGATG GACATTATGC CGCTGATTCA 
GTTGCAGCTG CAATTGCTGT TGGTGCTGTA GCAGCCGAAA CAGCTCAAGA AATAGGTTTC 
CAAGGCGGGT CATTAGTGTC TTCTCCATTG GGTAAGGCTA CCTCAAAGGC CCCTCCATTG 
CAGGGAAAAT CGAGTGATGC ATCAAGCTTG ATGAACATGT CAAGAAACTT CAGGGCCGAT 
GTCTTGAGAG AAAATACAGA AGCTGTCTTT GCGGCTTGTG AGGTTACTCA TGGTAGGTGG 
GCAAAGCTAC TAGGAGTTCG CGCTCTTCTT CATCCAAAAT TGAAATTGCA AGAGTTCATG 
AGCATTTATG ATCTGACCCA AGAATTTATA ACATCTACGG AGAAGATTGG TGGAAGGTTG 
GGTTCTAGTA TTCGTGGGAC GCTGCAATCA CAAGCCAAAG CCTTTGTGGA CTCCCAGCAC 
GAATCTCGGA TGACAAAACT AAAAGCTGTG CTTGATCAAG AAACATGGGA TGAGATAGAT 
GTTCCTGAAG AATTTCAATC CATAATCAGT TCTCTTTTTG CATCCCAGCG GTTGATCTCT 
GGGAAAGTTG ATGATGCTGA TCTTAATTCA TACCACAGTA ATCGGCTCCC TCTCAATGGG 
TCTCTCACTT CTGGATCTGG GGATCAAAAT AGTGAATTAC GAAATGAAAA ATCTGAATCT 
AGTGAAGGTT CTGTAGTTAG TGATGCACAA GTGAAACCCA CAGTCTCACC TGAGTCTCTT 
GAAAGAAGCA AGGCTGGTGT ATCTTCAGCT ACGAATAATC AAAGTAATCA GAAGGCGCAC 
GGAAAATCTA ACCTTTTCTA TCAAGGAGTT GGTTACCACA TGGTAAACTG TGGGTTAATA 
TTGCTCAAGA TGCTGTCGGA ATACATTGAT ATGAACAATT CTTTGCCAGC TTTGTCCTCG 
GAAATTGTTC TTCGTGTTGT TGAAGTGTTG AGATTTTTCA ACACTCGGAC ATGCCAACTT 
GTTCTGGGTG CTGGTGCTAT GCAGGTATCT GGTTTGAAGT CCATAAAGGC GAAACACTTG 
GCGCTTGCAA GTCAAGTAAT CGACTTCACT TACACTATTA TTCCTGAAAC CAGAAGGATT 
CTGTTTTCAA AAGTACCTGA GACGAGAAAG CCTCTGTTGT CAGTAGAGAT TGATAAAGTT 
GCTCAGGATT TTAGAATTCA TCGAGATGAA ATATACACAA AACTGGTTCA GATTATGAGA 
GAAAGACTAT TGGCGCATCT TCATGGTTTG CCCAAAGTTG TTGAGGGCTG GAACAGACCT 
CCAGATACCA ATAAACAAAC TAAAGAGTTT GCATGGCCGC TCACAAGGGA AGTTGGCTAC 
CTTCACCGTG TGTTGTCTGA GACATTGCAC GAAGCCGATG TTCAAGCAAT CTTCAGGCAG 
GTGATTTCAA TTATCCACAC ACAAACCTCT CAAACGCTTA CGAACTTGGA GATAAGCTCA 
ACAGAGGCAA AGAAAAGGCT GAAGCTTCAT GTCGAGCTAA TCCTCAAATG CATCCGGTCG 
TTACCATCCG ACAATGCCAA TCAATCAGAC ATCCCAAACT GGGGACAACT AGATGAATTC 
TTTGCAGAGC ACTTTAGAGA AGAAGAAGCA GGCGAAGCTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_849409.2
CDS	322..3426
Translation	MDSHPSLMGRSITNSNRSSLDLGRPSSSSSSSPSPLTKSISDASSQSLSSILNNPHGGKSGVYGSDASWVGWWSSSTFVAPAEFAPVASTKLPGSELTRSDFHGYVSSISESHGRFEDIRKHTREESCGFDQESHVSGLAACLREVPSLYFKEDFALEDGATFRSACPFSSLNENLALQEKLSQYLDVVELHLVKEISVRSDSFFEAQGQLQDLNVKIVEGCSRIRELKETIRLLDRNLVDSARQIQELSSTRINMLELQRKLRLILYVNQALSALKLLVASADCAGALDITDDLQNLLAGDELTGLYCFRHLRDHVTSSIDSINSILTSEFMRISIHDTGEIDVLILSAANIRGSISSNGNTGEEVKLEEEDTSTLCDRLLPLVIGLLRTAKFPSILRMYRDTLTSEMKNAIKKAVADLLPILVARSLESDFSHGERSVDDGGGLSLASKLRTLSSEAFVNLLTAIFKIVQAHLVRASEVKKAIEWILCNIDGHYAADSVAAAIAVGAVAAETAQEIGFQGGSLVSSPLGKATSKAPPLQGKSSDASSLMNMSRNFRADVLRENTEAVFAACEVTHGRWAKLLGVRALLHPKLKLQEFMSIYDLTQEFITSTEKIGGRLGSSIRGTLQSQAKAFVDSQHESRMTKLKAVLDQETWDEIDVPEEFQSIISSLFASQRLISGKVDDADLNSYHSNRLPLNGSLTSGSGDQNSELRNEKSESSEGSVVSDAQVKPTVSPESLERSKAGVSSATNNQSNQKAHGKSNLFYQGVGYHMVNCGLILLKMLSEYIDMNNSLPALSSEIVLRVVEVLRFFNTRTCQLVLGAGAMQVSGLKSIKAKHLALASQVIDFTYTIIPETRRILFSKVPETRKPLLSVEIDKVAQDFRIHRDEIYTKLVQIMRERLLAHLHGLPKVVEGWNRPPDTNKQTKEFAWPLTREVGYLHRVLSETLHEADVQAIFRQVISIIHTQTSQTLTNLEISSTEAKKRLKLHVELILKCIRSLPSDNANQSDIPNWGQLDEFFAEHFREEEAGEAE

Target ORF information:

RefSeq Version	NM_179078.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana VPS54 (VPS54), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_179078.3

ORF Insert Sequence:

ATGGATTCAC ACCCTTCCCT AATGGGAAGG TCCATTACCA ATTCGAACCG ATCGAGCTTA 
GATCTTGGTC GTCCTTCTTC GTCTTCTTCA TCTTCTCCTT CACCGTTAAC CAAATCGATC 
TCCGATGCAA GTAGTCAAAG TCTCTCTTCG ATCCTTAACA ATCCACACGG AGGTAAGTCT 
GGTGTTTATG GTTCCGATGC ATCTTGGGTT GGTTGGTGGT CTTCATCGAC ATTTGTAGCT 
CCAGCTGAGT TTGCGCCTGT TGCTTCAACG AAGCTCCCTG GTTCGGAGCT AACCAGATCC 
GACTTCCATG GCTACGTTTC TTCGATCTCT GAGTCTCATG GTAGATTTGA GGATATTAGG 
AAGCATACTA GGGAAGAGAG CTGTGGGTTT GATCAGGAGA GCCATGTATC GGGTTTAGCT 
GCGTGTTTGC GTGAGGTTCC TTCGCTTTAC TTTAAGGAGG ATTTTGCTCT TGAGGACGGA 
GCTACATTTC GTTCTGCTTG CCCTTTCTCG AGCCTTAATG AGAATCTAGC GCTTCAAGAG 
AAGTTGTCAC AGTATCTTGA TGTGGTGGAG CTGCATCTTG TGAAGGAGAT CTCTGTGAGG 
TCTGATTCGT TTTTTGAGGC TCAAGGACAG TTACAGGATT TGAATGTGAA GATTGTTGAG 
GGTTGTAGTA GGATTCGTGA GTTGAAAGAG ACTATTCGGC TCCTGGATAG GAATTTGGTA 
GATTCAGCTC GACAGATTCA AGAGCTGAGC TCAACTAGGA TTAATATGCT GGAGCTGCAA 
CGTAAATTAA GACTTATTTT GTATGTTAAC CAAGCTCTCT CTGCACTCAA ATTGCTTGTT 
GCGTCCGCAG ATTGTGCTGG AGCTTTGGAT ATAACTGACG ATCTCCAGAA CTTGCTGGCT 
GGGGATGAGC TTACTGGTCT ATACTGCTTC CGCCATCTTC GGGATCATGT TACTAGTTCC 
ATAGATTCCA TAAACAGTAT TCTCACTTCA GAATTCATGC GTATCTCCAT ACATGATACT 
GGGGAGATAG ATGTACTGAT TTTATCTGCA GCAAATATAC GGGGATCCAT TTCATCTAAC 
GGGAATACCG GCGAAGAAGT TAAGTTAGAA GAAGAGGATA CCTCTACCTT ATGTGATCGG 
CTTCTTCCTT TAGTTATTGG ATTGCTAAGA ACGGCAAAGT TCCCGTCAAT TTTGAGGATG 
TATCGTGATA CACTCACATC CGAAATGAAA AATGCAATCA AGAAAGCAGT TGCTGATCTG 
CTTCCCATTC TTGTTGCAAG ATCGCTCGAA TCTGATTTTT CACATGGGGA GCGCTCTGTA 
GATGATGGTG GTGGCTTATC TCTCGCGAGC AAATTGAGGA CTCTGTCATC TGAGGCTTTT 
GTTAATCTCT TAACTGCTAT ATTTAAGATT GTTCAGGCAC ATCTAGTCCG GGCTTCGGAA 
GTGAAAAAAG CAATTGAATG GATTCTCTGC AATATCGATG GACATTATGC CGCTGATTCA 
GTTGCAGCTG CAATTGCTGT TGGTGCTGTA GCAGCCGAAA CAGCTCAAGA AATAGGTTTC 
CAAGGCGGGT CATTAGTGTC TTCTCCATTG GGTAAGGCTA CCTCAAAGGC CCCTCCATTG 
CAGGGAAAAT CGAGTGATGC ATCAAGCTTG ATGAACATGT CAAGAAACTT CAGGGCCGAT 
GTCTTGAGAG AAAATACAGA AGCTGTCTTT GCGGCTTGTG AGGTTACTCA TGGTAGGTGG 
GCAAAGCTAC TAGGAGTTCG CGCTCTTCTT CATCCAAAAT TGAAATTGCA AGAGTTCATG 
AGCATTTATG ATCTGACCCA AGAATTTATA ACATCTACGG AGAAGATTGG TGGAAGGTTG 
GGTTCTAGTA TTCGTGGGAC GCTGCAATCA CAAGCCAAAG CCTTTGTGGA CTCCCAGCAC 
GAATCTCGGA TGACAAAACT AAAAGCTGTG CTTGATCAAG AAACATGGGA TGAGATAGAT 
GTTCCTGAAG AATTTCAATC CATAATCAGT TCTCTTTTTG CATCCCAGCG GTTGATCTCT 
GGGAAAGTTG ATGATGCTGA TCTTAATTCA TACCACAGTA ATCGGCTCCC TCTCAATGGG 
TCTCTCACTT CTGGATCTGG GGATCAAAAT AGTGAATTAC GAAATGAAAA ATCTGAATCT 
AGTGAAGGTT CTGTAGTTAG TGATGCACAA GTGAAACCCA CAGTCTCACC TGAGTCTCTT 
GAAAGAAGCA AGGCTGGTGT ATCTTCAGCT ACGAATAATC AAAGTAATCA GAAGGCGCAC 
GGAAAATCTA ACCTTTTCTA TCAAGGAGTT GGTTACCACA TGGTAAACTG TGGGTTAATA 
TTGCTCAAGA TGCTGTCGGA ATACATTGAT ATGAACAATT CTTTGCCAGC TTTGTCCTCG 
GAAATTGTTC TTCGTGTTGT TGAAGTGTTG AGATTTTTCA ACACTCGGAC ATGCCAACTT 
GTTCTGGGTG CTGGTGCTAT GCAGGTATCT GGTTTGAAGT CCATAAAGGC GAAACACTTG 
GCGCTTGCAA GTCAAGTAAT CGACTTCACT TACACTATTA TTCCTGAAAC CAGAAGGATT 
CTGTTTTCAA AAGTACCTGA GACGAGAAAG CCTCTGTTGT CAGTAGAGAT TGATAAAGTT 
GCTCAGGATT TTAGAATTCA TCGAGATGAA ATATACACAA AACTGGTTCA GATTATGAGA 
GAAAGACTAT TGGCGCATCT TCATGGTTTG CCCAAAGTTG TTGAGGGCTG GAACAGACCT 
CCAGATACCA ATAAACAAAC TAAAGAGTTT GCATGGCCGC TCACAAGGGA AGTTGGCTAC 
CTTCACCGTG TGTTGTCTGA GACATTGCAC GAAGCCGATG TTCAAGCAAT CTTCAGGCAG 
GTGATTTCAA TTATCCACAC ACAAACCTCT CAAACGCTTA CGAACTTGGA GATAAGCTCA 
ACAGAGGCAA AGAAAAGGCT GAAGCTTCAT GTCGAGCTAA TCCTCAAATG CATCCGGTCG 
TTACCATCCG ACAATGCCAA TCAATCAGAC ATCCCAAACT GGGGACAACT AGATGAATTC 
TTTGCAGAGC ACTTTAGAGA AGAAGAAGCA GGCGAAGCTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS54 ( NM_118069.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_193684.4 Arabidopsis thaliana VPS54 (VPS54), mRNA.

Price & Availability↑

CloneID	OAb15393
Clone ID Related Accession (Same CDS sequence)	NM_179078.3 , NM_118069.5
Accession Version	NM_118069.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	VPS54
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_118069.4.

ATGGATTCAC ACCCTTCCCT AATGGGAAGG TCCATTACCA ATTCGAACCG ATCGAGCTTA 
GATCTTGGTC GTCCTTCTTC GTCTTCTTCA TCTTCTCCTT CACCGTTAAC CAAATCGATC 
TCCGATGCAA GTAGTCAAAG TCTCTCTTCG ATCCTTAACA ATCCACACGG AGGTAAGTCT 
GGTGTTTATG GTTCCGATGC ATCTTGGGTT GGTTGGTGGT CTTCATCGAC ATTTGTAGCT 
CCAGCTGAGT TTGCGCCTGT TGCTTCAACG AAGCTCCCTG GTTCGGAGCT AACCAGATCC 
GACTTCCATG GCTACGTTTC TTCGATCTCT GAGTCTCATG GTAGATTTGA GGATATTAGG 
AAGCATACTA GGGAAGAGAG CTGTGGGTTT GATCAGGAGA GCCATGTATC GGGTTTAGCT 
GCGTGTTTGC GTGAGGTTCC TTCGCTTTAC TTTAAGGAGG ATTTTGCTCT TGAGGACGGA 
GCTACATTTC GTTCTGCTTG CCCTTTCTCG AGCCTTAATG AGAATCTAGC GCTTCAAGAG 
AAGTTGTCAC AGTATCTTGA TGTGGTGGAG CTGCATCTTG TGAAGGAGAT CTCTGTGAGG 
TCTGATTCGT TTTTTGAGGC TCAAGGACAG TTACAGGATT TGAATGTGAA GATTGTTGAG 
GGTTGTAGTA GGATTCGTGA GTTGAAAGAG ACTATTCGGC TCCTGGATAG GAATTTGGTA 
GATTCAGCTC GACAGATTCA AGAGCTGAGC TCAACTAGGA TTAATATGCT GGAGCTGCAA 
CGTAAATTAA GACTTATTTT GTATGTTAAC CAAGCTCTCT CTGCACTCAA ATTGCTTGTT 
GCGTCCGCAG ATTGTGCTGG AGCTTTGGAT ATAACTGACG ATCTCCAGAA CTTGCTGGCT 
GGGGATGAGC TTACTGGTCT ATACTGCTTC CGCCATCTTC GGGATCATGT TACTAGTTCC 
ATAGATTCCA TAAACAGTAT TCTCACTTCA GAATTCATGC GTATCTCCAT ACATGATACT 
GGGGAGATAG ATGTACTGAT TTTATCTGCA GCAAATATAC GGGGATCCAT TTCATCTAAC 
GGGAATACCG GCGAAGAAGT TAAGTTAGAA GAAGAGGATA CCTCTACCTT ATGTGATCGG 
CTTCTTCCTT TAGTTATTGG ATTGCTAAGA ACGGCAAAGT TCCCGTCAAT TTTGAGGATG 
TATCGTGATA CACTCACATC CGAAATGAAA AATGCAATCA AGAAAGCAGT TGCTGATCTG 
CTTCCCATTC TTGTTGCAAG ATCGCTCGAA TCTGATTTTT CACATGGGGA GCGCTCTGTA 
GATGATGGTG GTGGCTTATC TCTCGCGAGC AAATTGAGGA CTCTGTCATC TGAGGCTTTT 
GTTAATCTCT TAACTGCTAT ATTTAAGATT GTTCAGGCAC ATCTAGTCCG GGCTTCGGAA 
GTGAAAAAAG CAATTGAATG GATTCTCTGC AATATCGATG GACATTATGC CGCTGATTCA 
GTTGCAGCTG CAATTGCTGT TGGTGCTGTA GCAGCCGAAA CAGCTCAAGA AATAGGTTTC 
CAAGGCGGGT CATTAGTGTC TTCTCCATTG GGTAAGGCTA CCTCAAAGGC CCCTCCATTG 
CAGGGAAAAT CGAGTGATGC ATCAAGCTTG ATGAACATGT CAAGAAACTT CAGGGCCGAT 
GTCTTGAGAG AAAATACAGA AGCTGTCTTT GCGGCTTGTG AGGTTACTCA TGGTAGGTGG 
GCAAAGCTAC TAGGAGTTCG CGCTCTTCTT CATCCAAAAT TGAAATTGCA AGAGTTCATG 
AGCATTTATG ATCTGACCCA AGAATTTATA ACATCTACGG AGAAGATTGG TGGAAGGTTG 
GGTTCTAGTA TTCGTGGGAC GCTGCAATCA CAAGCCAAAG CCTTTGTGGA CTCCCAGCAC 
GAATCTCGGA TGACAAAACT AAAAGCTGTG CTTGATCAAG AAACATGGGA TGAGATAGAT 
GTTCCTGAAG AATTTCAATC CATAATCAGT TCTCTTTTTG CATCCCAGCG GTTGATCTCT 
GGGAAAGTTG ATGATGCTGA TCTTAATTCA TACCACAGTA ATCGGCTCCC TCTCAATGGG 
TCTCTCACTT CTGGATCTGG GGATCAAAAT AGTGAATTAC GAAATGAAAA ATCTGAATCT 
AGTGAAGGTT CTGTAGTTAG TGATGCACAA GTGAAACCCA CAGTCTCACC TGAGTCTCTT 
GAAAGAAGCA AGGCTGGTGT ATCTTCAGCT ACGAATAATC AAAGTAATCA GAAGGCGCAC 
GGAAAATCTA ACCTTTTCTA TCAAGGAGTT GGTTACCACA TGGTAAACTG TGGGTTAATA 
TTGCTCAAGA TGCTGTCGGA ATACATTGAT ATGAACAATT CTTTGCCAGC TTTGTCCTCG 
GAAATTGTTC TTCGTGTTGT TGAAGTGTTG AGATTTTTCA ACACTCGGAC ATGCCAACTT 
GTTCTGGGTG CTGGTGCTAT GCAGGTATCT GGTTTGAAGT CCATAAAGGC GAAACACTTG 
GCGCTTGCAA GTCAAGTAAT CGACTTCACT TACACTATTA TTCCTGAAAC CAGAAGGATT 
CTGTTTTCAA AAGTACCTGA GACGAGAAAG CCTCTGTTGT CAGTAGAGAT TGATAAAGTT 
GCTCAGGATT TTAGAATTCA TCGAGATGAA ATATACACAA AACTGGTTCA GATTATGAGA 
GAAAGACTAT TGGCGCATCT TCATGGTTTG CCCAAAGTTG TTGAGGGCTG GAACAGACCT 
CCAGATACCA ATAAACAAAC TAAAGAGTTT GCATGGCCGC TCACAAGGGA AGTTGGCTAC 
CTTCACCGTG TGTTGTCTGA GACATTGCAC GAAGCCGATG TTCAAGCAAT CTTCAGGCAG 
GTGATTTCAA TTATCCACAC ACAAACCTCT CAAACGCTTA CGAACTTGGA GATAAGCTCA 
ACAGAGGCAA AGAAAAGGCT GAAGCTTCAT GTCGAGCTAA TCCTCAAATG CATCCGGTCG 
TTACCATCCG ACAATGCCAA TCAATCAGAC ATCCCAAACT GGGGACAACT AGATGAATTC 
TTTGCAGAGC ACTTTAGAGA AGAAGAAGCA GGCGAAGCTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_193684.4
CDS	322..3426
Translation	MDSHPSLMGRSITNSNRSSLDLGRPSSSSSSSPSPLTKSISDASSQSLSSILNNPHGGKSGVYGSDASWVGWWSSSTFVAPAEFAPVASTKLPGSELTRSDFHGYVSSISESHGRFEDIRKHTREESCGFDQESHVSGLAACLREVPSLYFKEDFALEDGATFRSACPFSSLNENLALQEKLSQYLDVVELHLVKEISVRSDSFFEAQGQLQDLNVKIVEGCSRIRELKETIRLLDRNLVDSARQIQELSSTRINMLELQRKLRLILYVNQALSALKLLVASADCAGALDITDDLQNLLAGDELTGLYCFRHLRDHVTSSIDSINSILTSEFMRISIHDTGEIDVLILSAANIRGSISSNGNTGEEVKLEEEDTSTLCDRLLPLVIGLLRTAKFPSILRMYRDTLTSEMKNAIKKAVADLLPILVARSLESDFSHGERSVDDGGGLSLASKLRTLSSEAFVNLLTAIFKIVQAHLVRASEVKKAIEWILCNIDGHYAADSVAAAIAVGAVAAETAQEIGFQGGSLVSSPLGKATSKAPPLQGKSSDASSLMNMSRNFRADVLRENTEAVFAACEVTHGRWAKLLGVRALLHPKLKLQEFMSIYDLTQEFITSTEKIGGRLGSSIRGTLQSQAKAFVDSQHESRMTKLKAVLDQETWDEIDVPEEFQSIISSLFASQRLISGKVDDADLNSYHSNRLPLNGSLTSGSGDQNSELRNEKSESSEGSVVSDAQVKPTVSPESLERSKAGVSSATNNQSNQKAHGKSNLFYQGVGYHMVNCGLILLKMLSEYIDMNNSLPALSSEIVLRVVEVLRFFNTRTCQLVLGAGAMQVSGLKSIKAKHLALASQVIDFTYTIIPETRRILFSKVPETRKPLLSVEIDKVAQDFRIHRDEIYTKLVQIMRERLLAHLHGLPKVVEGWNRPPDTNKQTKEFAWPLTREVGYLHRVLSETLHEADVQAIFRQVISIIHTQTSQTLTNLEISSTEAKKRLKLHVELILKCIRSLPSDNANQSDIPNWGQLDEFFAEHFREEEAGEAE

Target ORF information:

RefSeq Version	NM_118069.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana VPS54 (VPS54), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_118069.5

ORF Insert Sequence:

ATGGATTCAC ACCCTTCCCT AATGGGAAGG TCCATTACCA ATTCGAACCG ATCGAGCTTA 
GATCTTGGTC GTCCTTCTTC GTCTTCTTCA TCTTCTCCTT CACCGTTAAC CAAATCGATC 
TCCGATGCAA GTAGTCAAAG TCTCTCTTCG ATCCTTAACA ATCCACACGG AGGTAAGTCT 
GGTGTTTATG GTTCCGATGC ATCTTGGGTT GGTTGGTGGT CTTCATCGAC ATTTGTAGCT 
CCAGCTGAGT TTGCGCCTGT TGCTTCAACG AAGCTCCCTG GTTCGGAGCT AACCAGATCC 
GACTTCCATG GCTACGTTTC TTCGATCTCT GAGTCTCATG GTAGATTTGA GGATATTAGG 
AAGCATACTA GGGAAGAGAG CTGTGGGTTT GATCAGGAGA GCCATGTATC GGGTTTAGCT 
GCGTGTTTGC GTGAGGTTCC TTCGCTTTAC TTTAAGGAGG ATTTTGCTCT TGAGGACGGA 
GCTACATTTC GTTCTGCTTG CCCTTTCTCG AGCCTTAATG AGAATCTAGC GCTTCAAGAG 
AAGTTGTCAC AGTATCTTGA TGTGGTGGAG CTGCATCTTG TGAAGGAGAT CTCTGTGAGG 
TCTGATTCGT TTTTTGAGGC TCAAGGACAG TTACAGGATT TGAATGTGAA GATTGTTGAG 
GGTTGTAGTA GGATTCGTGA GTTGAAAGAG ACTATTCGGC TCCTGGATAG GAATTTGGTA 
GATTCAGCTC GACAGATTCA AGAGCTGAGC TCAACTAGGA TTAATATGCT GGAGCTGCAA 
CGTAAATTAA GACTTATTTT GTATGTTAAC CAAGCTCTCT CTGCACTCAA ATTGCTTGTT 
GCGTCCGCAG ATTGTGCTGG AGCTTTGGAT ATAACTGACG ATCTCCAGAA CTTGCTGGCT 
GGGGATGAGC TTACTGGTCT ATACTGCTTC CGCCATCTTC GGGATCATGT TACTAGTTCC 
ATAGATTCCA TAAACAGTAT TCTCACTTCA GAATTCATGC GTATCTCCAT ACATGATACT 
GGGGAGATAG ATGTACTGAT TTTATCTGCA GCAAATATAC GGGGATCCAT TTCATCTAAC 
GGGAATACCG GCGAAGAAGT TAAGTTAGAA GAAGAGGATA CCTCTACCTT ATGTGATCGG 
CTTCTTCCTT TAGTTATTGG ATTGCTAAGA ACGGCAAAGT TCCCGTCAAT TTTGAGGATG 
TATCGTGATA CACTCACATC CGAAATGAAA AATGCAATCA AGAAAGCAGT TGCTGATCTG 
CTTCCCATTC TTGTTGCAAG ATCGCTCGAA TCTGATTTTT CACATGGGGA GCGCTCTGTA 
GATGATGGTG GTGGCTTATC TCTCGCGAGC AAATTGAGGA CTCTGTCATC TGAGGCTTTT 
GTTAATCTCT TAACTGCTAT ATTTAAGATT GTTCAGGCAC ATCTAGTCCG GGCTTCGGAA 
GTGAAAAAAG CAATTGAATG GATTCTCTGC AATATCGATG GACATTATGC CGCTGATTCA 
GTTGCAGCTG CAATTGCTGT TGGTGCTGTA GCAGCCGAAA CAGCTCAAGA AATAGGTTTC 
CAAGGCGGGT CATTAGTGTC TTCTCCATTG GGTAAGGCTA CCTCAAAGGC CCCTCCATTG 
CAGGGAAAAT CGAGTGATGC ATCAAGCTTG ATGAACATGT CAAGAAACTT CAGGGCCGAT 
GTCTTGAGAG AAAATACAGA AGCTGTCTTT GCGGCTTGTG AGGTTACTCA TGGTAGGTGG 
GCAAAGCTAC TAGGAGTTCG CGCTCTTCTT CATCCAAAAT TGAAATTGCA AGAGTTCATG 
AGCATTTATG ATCTGACCCA AGAATTTATA ACATCTACGG AGAAGATTGG TGGAAGGTTG 
GGTTCTAGTA TTCGTGGGAC GCTGCAATCA CAAGCCAAAG CCTTTGTGGA CTCCCAGCAC 
GAATCTCGGA TGACAAAACT AAAAGCTGTG CTTGATCAAG AAACATGGGA TGAGATAGAT 
GTTCCTGAAG AATTTCAATC CATAATCAGT TCTCTTTTTG CATCCCAGCG GTTGATCTCT 
GGGAAAGTTG ATGATGCTGA TCTTAATTCA TACCACAGTA ATCGGCTCCC TCTCAATGGG 
TCTCTCACTT CTGGATCTGG GGATCAAAAT AGTGAATTAC GAAATGAAAA ATCTGAATCT 
AGTGAAGGTT CTGTAGTTAG TGATGCACAA GTGAAACCCA CAGTCTCACC TGAGTCTCTT 
GAAAGAAGCA AGGCTGGTGT ATCTTCAGCT ACGAATAATC AAAGTAATCA GAAGGCGCAC 
GGAAAATCTA ACCTTTTCTA TCAAGGAGTT GGTTACCACA TGGTAAACTG TGGGTTAATA 
TTGCTCAAGA TGCTGTCGGA ATACATTGAT ATGAACAATT CTTTGCCAGC TTTGTCCTCG 
GAAATTGTTC TTCGTGTTGT TGAAGTGTTG AGATTTTTCA ACACTCGGAC ATGCCAACTT 
GTTCTGGGTG CTGGTGCTAT GCAGGTATCT GGTTTGAAGT CCATAAAGGC GAAACACTTG 
GCGCTTGCAA GTCAAGTAAT CGACTTCACT TACACTATTA TTCCTGAAAC CAGAAGGATT 
CTGTTTTCAA AAGTACCTGA GACGAGAAAG CCTCTGTTGT CAGTAGAGAT TGATAAAGTT 
GCTCAGGATT TTAGAATTCA TCGAGATGAA ATATACACAA AACTGGTTCA GATTATGAGA 
GAAAGACTAT TGGCGCATCT TCATGGTTTG CCCAAAGTTG TTGAGGGCTG GAACAGACCT 
CCAGATACCA ATAAACAAAC TAAAGAGTTT GCATGGCCGC TCACAAGGGA AGTTGGCTAC 
CTTCACCGTG TGTTGTCTGA GACATTGCAC GAAGCCGATG TTCAAGCAAT CTTCAGGCAG 
GTGATTTCAA TTATCCACAC ACAAACCTCT CAAACGCTTA CGAACTTGGA GATAAGCTCA 
ACAGAGGCAA AGAAAAGGCT GAAGCTTCAT GTCGAGCTAA TCCTCAAATG CATCCGGTCG 
TTACCATCCG ACAATGCCAA TCAATCAGAC ATCCCAAACT GGGGACAACT AGATGAATTC 
TTTGCAGAGC ACTTTAGAGA AGAAGAAGCA GGCGAAGCTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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VPS54 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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