PLDGAMMA3 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLDGAMMA3
Entrez Gene ID	826790
Full Name	phospholipase D gamma 3
Synonyms	T26M18.50,T26M18_50,phospholipase D gamma 3
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001340738.1	NP_001329644.1	phospholipase D gamma 3
NM_117254.3	NP_192921.1	phospholipase D gamma 3

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	PLDGAMMA2	NP_192920.3
Arabidopsis thaliana (thale cress)	PLDGAMMA3	NP_192921.1
Arabidopsis thaliana (thale cress)	PLDBETA2	NP_567160.1
Oryza sativa (rice)	Os10g0524400	NP_001065108.1
Oryza sativa (rice)	Os03g0119100	NP_001048777.1
Arabidopsis thaliana (thale cress)	PLDBETA1	NP_565963.2
Arabidopsis thaliana (thale cress)	PLDGAMMA1	NP_192922.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PLDGAMMA3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLDGAMMA3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb14669	NM_117254.3 Latest version!	Arabidopsis thaliana phospholipase D gamma 3 (PLDGAMMA3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb39155	NM_001340738.1 Latest version!	Arabidopsis thaliana phospholipase D gamma 3 (PLDGAMMA3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLDGAMMA3 ( NM_117254.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_192921.1 Arabidopsis thaliana phospholipase D gamma 3 (PLDGAMMA3), mRNA.

Price & Availability↑

CloneID	OAb14669
Clone ID Related Accession (Same CDS sequence)	NM_117254.3
Accession Version	NM_117254.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	phospholipase D gamma 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_117254.2.

ATGGCGTATC ATCCAGTTTA TAACGAGACT ATGTCTATGG GAGGAGGGTC AAGCAACGAG 
TTTGGCCAGT GGCTTGACAA GCAACTCGTG CCGTTTGATA CAAGTAGTGG CTCTTTGAGG 
GTTGAATTAT TACATGGTAA CTTAGACATT TGGGTTAAGG AAGCTAAACA TCTTCCTAAC 
ATGGATGGTT TCCATAACAC CCTTGTTGGT GGGATGTTTT TTGGGTTAGG TCGGAGGAAT 
CATAAAGTGG ATGGTGAGAA TTCTTCTAAG ATCACAAGTG ATCCTTATGT TACTGTCTCT 
ATCTCTGGTG CTGTGATTGG TAGAACTTTT GTTATTAGCA ATAGTGAGAA TCCTGTGTGG 
ATGCAGCATT TTGATGTACC AGTTGCTCAT AGTGCTGCGA AAGTTCACTT TGTTGTGAAA 
GACAGTGATA TTATTGGATC ACAGATCATT GGAGCTGTTG AAATCCCAAC CGAGCAGTTG 
TGTTCCGGGA ATAGAATCGA AGGGCTGTTT CCGATACTTA ACAGTAGAGG AAAGCCTTGT 
AAACAAGGTG CTGTGTTGAG TCTGTCTATT CAGTATATTC CAATGGAAAG AATGAGACTT 
TACCAAAAGG GTGTTGGTTT TGGTGTTGAA TGTGTAGGAG TTCCCGGTAC ATACTTCCCT 
TTAAGGAAAG GCGGTAGGGT TACTCTTTAT CAGGATGCTC ATGTTGATGA CGGTACTCTT 
CCGAGTGTAC ATCTTGATGG TGGGATTCAG TATAGACATG GAAAATGCTG GGAAGATATG 
GCTGATGCGA TACGACGGGC AAGGAGGTTG ATTTATATCA CAGGTTGGTC AGTTTTCCAT 
CCGGTTAGGC TGGTTCGTCG TAACAATGAT CCGACCCAAG GTACATTAGG AGAGTTGCTT 
AAAGTCAAAT CTCAAGAAGG TGTTAGAGTG TTGGTTTTGG TGTGGGATGA TCCAACTTCA 
AGGAGTCTTT TGGGATTCAG TACAAAAGGA CTTATGAACA CAAGCGATGA GGAGACTCGC 
CGTTTTTTCA AGCACTCATC GGTGCAAGTT CTCCTTTGTC CTAGATATGG AGGGAAAGGT 
CACAGCTTCA TAAAAAAATC TGAAGTTGAA ACAATCTACA CGCATCATCA GAAAACTATG 
ATTGTAGATG CTGAAGCAGC TCAGAACCGA AGAAAGATTG TAGCATTTGT TGGAGGGCTA 
GACTTGTGCA ACGGACGATT TGATACTCCC AAGCATCCTC TCTTCAGGAC ATTAAAGACA 
ATCCATAAAG ATGACTTCCA TAACCCAAAT TTTGTGACTA CTGCGGATGA TGGACCAAGA 
GAACCATGGC ATGATCTGCA CAGCAAGATT GATGGTCCAG CTGCATATGA TGTGCTTGCT 
AATTTTGAGG AACGCTGGAT GAAGGCTTCA AAACCTCGCG GAATTGGGAG ACTAAGAACT 
TCTTCTGATG ATTCTTTGCT TAGGTTAGAC AGAATTCCTG ACATAATGGG ACTTTCCGAA 
GCTTCTTCTG CTAATGATAA TGATCCAGAG TCTTGGCATG TTCAGGTTTT CCGTTCAATT 
GATTCGAGTT CAGTTAAAGG GTTTCCAAAG GACCCAAAGG AAGCTACTGG AAGAAACCTT 
CTATGTGGGA AGAACATTCT CATAGATATG AGCATACATG CAGCTTATGT TAAAGCCATA 
CGATCTGCTC AGCACTTCAT TTACATTGAA AACCAATATT TTCTTGGATC ATCATTCAAT 
TGGGATTCAA ACAAGAACTT GGGTGCGAAT AATCTAATCC CGATGGAAAT CGCGCTGAAG 
ATTGCTAACA AAATTAGAGC AAGGGAAAAG TTTGCTGCTT ATATTGTCAT TCCAATGTGG 
CCAGAAGGTG CTCCAACAAG TAATCCTATT CAAAGAATTC TCTACTGGCA GCACAAAACC 
ATGCAAATGA TGTATCAAAC CATCTACAAG GCACTTGTGG AAGTTGGTCT TGATGGCCAG 
CTTGAGCCAC AAGACTTTTT GAACTTTTTC TGTCTTGGAA CCAGAGAGGT TGGAACCAGA 
GAGGTTCCTG ATGGAACTGT TAGCGTATAT AATAGTCCTC GGAAGCCACC TCAGCTAAAT 
GCTGCACAGG TACAAGCTTT GAAGAGTCGG AGATTCATGA TATATGTTCA TTCCAAAGGT 
ATGGTAGTGG ATGATGAGTT TGTCTTAATT GGTTCCGCGA ATATCAACCA GAGATCCTTG 
GAAGGAACTA GAGACACTGA AATCGCTATG GGAGGGTATC AGCCACACCA TTCATGGGCT 
AAGAAAGGTT CTCGTCCTCG TGGTCAGATC TTTGGATATA GAATGTCGTT ATGGGCAGAA 
CATCTAGGGT TTCTAGAGCA AGAATTCGAG GAACCAGAGA ACATGGAATG CGTGAGACGA 
GTTAGGCAAT TGAGTGAGCT GAATTGGAGA CAATATGCAG CGGAAGAAGT CACAGAGATG 
CCAGGTCATC TTCTTAAGTA TCCAGTTCAA GTTGATAGAA CAGGCAAAGT GAGTTCTCTT 
CCTGGATACG AGACATTCCC AGATCTCGGT GGCAAGATCA TAGGATCGTT CCTTGTAGTA 
GAAGAAAACC TCACCATATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_192921.1
CDS	436..3036
Translation	MAYHPVYNETMSMGGGSSNEFGQWLDKQLVPFDTSSGSLRVELLHGNLDIWVKEAKHLPNMDGFHNTLVGGMFFGLGRRNHKVDGENSSKITSDPYVTVSISGAVIGRTFVISNSENPVWMQHFDVPVAHSAAKVHFVVKDSDIIGSQIIGAVEIPTEQLCSGNRIEGLFPILNSRGKPCKQGAVLSLSIQYIPMERMRLYQKGVGFGVECVGVPGTYFPLRKGGRVTLYQDAHVDDGTLPSVHLDGGIQYRHGKCWEDMADAIRRARRLIYITGWSVFHPVRLVRRNNDPTQGTLGELLKVKSQEGVRVLVLVWDDPTSRSLLGFSTKGLMNTSDEETRRFFKHSSVQVLLCPRYGGKGHSFIKKSEVETIYTHHQKTMIVDAEAAQNRRKIVAFVGGLDLCNGRFDTPKHPLFRTLKTIHKDDFHNPNFVTTADDGPREPWHDLHSKIDGPAAYDVLANFEERWMKASKPRGIGRLRTSSDDSLLRLDRIPDIMGLSEASSANDNDPESWHVQVFRSIDSSSVKGFPKDPKEATGRNLLCGKNILIDMSIHAAYVKAIRSAQHFIYIENQYFLGSSFNWDSNKNLGANNLIPMEIALKIANKIRAREKFAAYIVIPMWPEGAPTSNPIQRILYWQHKTMQMMYQTIYKALVEVGLDGQLEPQDFLNFFCLGTREVGTREVPDGTVSVYNSPRKPPQLNAAQVQALKSRRFMIYVHSKGMVVDDEFVLIGSANINQRSLEGTRDTEIAMGGYQPHHSWAKKGSRPRGQIFGYRMSLWAEHLGFLEQEFEEPENMECVRRVRQLSELNWRQYAAEEVTEMPGHLLKYPVQVDRTGKVSSLPGYETFPDLGGKIIGSFLVVEENLTI

Target ORF information:

RefSeq Version	NM_117254.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana phospholipase D gamma 3 (PLDGAMMA3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_117254.3

ORF Insert Sequence:

ATGGCGTATC ATCCAGTTTA TAACGAGACT ATGTCTATGG GAGGAGGGTC AAGCAACGAG 
TTTGGCCAGT GGCTTGACAA GCAACTCGTG CCGTTTGATA CAAGTAGTGG CTCTTTGAGG 
GTTGAATTAT TACATGGTAA CTTAGACATT TGGGTTAAGG AAGCTAAACA TCTTCCTAAC 
ATGGATGGTT TCCATAACAC CCTTGTTGGT GGGATGTTTT TTGGGTTAGG TCGGAGGAAT 
CATAAAGTGG ATGGTGAGAA TTCTTCTAAG ATCACAAGTG ATCCTTATGT TACTGTCTCT 
ATCTCTGGTG CTGTGATTGG TAGAACTTTT GTTATTAGCA ATAGTGAGAA TCCTGTGTGG 
ATGCAGCATT TTGATGTACC AGTTGCTCAT AGTGCTGCGA AAGTTCACTT TGTTGTGAAA 
GACAGTGATA TTATTGGATC ACAGATCATT GGAGCTGTTG AAATCCCAAC CGAGCAGTTG 
TGTTCCGGGA ATAGAATCGA AGGGCTGTTT CCGATACTTA ACAGTAGAGG AAAGCCTTGT 
AAACAAGGTG CTGTGTTGAG TCTGTCTATT CAGTATATTC CAATGGAAAG AATGAGACTT 
TACCAAAAGG GTGTTGGTTT TGGTGTTGAA TGTGTAGGAG TTCCCGGTAC ATACTTCCCT 
TTAAGGAAAG GCGGTAGGGT TACTCTTTAT CAGGATGCTC ATGTTGATGA CGGTACTCTT 
CCGAGTGTAC ATCTTGATGG TGGGATTCAG TATAGACATG GAAAATGCTG GGAAGATATG 
GCTGATGCGA TACGACGGGC AAGGAGGTTG ATTTATATCA CAGGTTGGTC AGTTTTCCAT 
CCGGTTAGGC TGGTTCGTCG TAACAATGAT CCGACCCAAG GTACATTAGG AGAGTTGCTT 
AAAGTCAAAT CTCAAGAAGG TGTTAGAGTG TTGGTTTTGG TGTGGGATGA TCCAACTTCA 
AGGAGTCTTT TGGGATTCAG TACAAAAGGA CTTATGAACA CAAGCGATGA GGAGACTCGC 
CGTTTTTTCA AGCACTCATC GGTGCAAGTT CTCCTTTGTC CTAGATATGG AGGGAAAGGT 
CACAGCTTCA TAAAAAAATC TGAAGTTGAA ACAATCTACA CGCATCATCA GAAAACTATG 
ATTGTAGATG CTGAAGCAGC TCAGAACCGA AGAAAGATTG TAGCATTTGT TGGAGGGCTA 
GACTTGTGCA ACGGACGATT TGATACTCCC AAGCATCCTC TCTTCAGGAC ATTAAAGACA 
ATCCATAAAG ATGACTTCCA TAACCCAAAT TTTGTGACTA CTGCGGATGA TGGACCAAGA 
GAACCATGGC ATGATCTGCA CAGCAAGATT GATGGTCCAG CTGCATATGA TGTGCTTGCT 
AATTTTGAGG AACGCTGGAT GAAGGCTTCA AAACCTCGCG GAATTGGGAG ACTAAGAACT 
TCTTCTGATG ATTCTTTGCT TAGGTTAGAC AGAATTCCTG ACATAATGGG ACTTTCCGAA 
GCTTCTTCTG CTAATGATAA TGATCCAGAG TCTTGGCATG TTCAGGTTTT CCGTTCAATT 
GATTCGAGTT CAGTTAAAGG GTTTCCAAAG GACCCAAAGG AAGCTACTGG AAGAAACCTT 
CTATGTGGGA AGAACATTCT CATAGATATG AGCATACATG CAGCTTATGT TAAAGCCATA 
CGATCTGCTC AGCACTTCAT TTACATTGAA AACCAATATT TTCTTGGATC ATCATTCAAT 
TGGGATTCAA ACAAGAACTT GGGTGCGAAT AATCTAATCC CGATGGAAAT CGCGCTGAAG 
ATTGCTAACA AAATTAGAGC AAGGGAAAAG TTTGCTGCTT ATATTGTCAT TCCAATGTGG 
CCAGAAGGTG CTCCAACAAG TAATCCTATT CAAAGAATTC TCTACTGGCA GCACAAAACC 
ATGCAAATGA TGTATCAAAC CATCTACAAG GCACTTGTGG AAGTTGGTCT TGATGGCCAG 
CTTGAGCCAC AAGACTTTTT GAACTTTTTC TGTCTTGGAA CCAGAGAGGT TGGAACCAGA 
GAGGTTCCTG ATGGAACTGT TAGCGTATAT AATAGTCCTC GGAAGCCACC TCAGCTAAAT 
GCTGCACAGG TACAAGCTTT GAAGAGTCGG AGATTCATGA TATATGTTCA TTCCAAAGGT 
ATGGTAGTGG ATGATGAGTT TGTCTTAATT GGTTCCGCGA ATATCAACCA GAGATCCTTG 
GAAGGAACTA GAGACACTGA AATCGCTATG GGAGGGTATC AGCCACACCA TTCATGGGCT 
AAGAAAGGTT CTCGTCCTCG TGGTCAGATC TTTGGATATA GAATGTCGTT ATGGGCAGAA 
CATCTAGGGT TTCTAGAGCA AGAATTCGAG GAACCAGAGA ACATGGAATG CGTGAGACGA 
GTTAGGCAAT TGAGTGAGCT GAATTGGAGA CAATATGCAG CGGAAGAAGT CACAGAGATG 
CCAGGTCATC TTCTTAAGTA TCCAGTTCAA GTTGATAGAA CAGGCAAAGT GAGTTCTCTT 
CCTGGATACG AGACATTCCC AGATCTCGGT GGCAAGATCA TAGGATCGTT CCTTGTAGTA 
GAAGAAAACC TCACCATATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLDGAMMA3 ( NM_001340738.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001329644.1 Arabidopsis thaliana phospholipase D gamma 3 (PLDGAMMA3), mRNA.

Price & Availability↑

CloneID	OAb39155
Clone ID Related Accession (Same CDS sequence)	NM_001340738.1
Accession Version	NM_001340738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	phospholipase D gamma 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGGCGTATC ATCCAGTTTA TAACGAGACT ATGTCTATGG GAGGAGGGTC AAGCAACGAG 
TTTGGCCAGT GGCTTGACAA GCAACTCGTG CCGTTTGATA CAAGTAGTGG CTCTTTGAGG 
GTTGAATTAT TACATGGTAA CTTAGACATT TGGGTTAAGG AAGCTAAACA TCTTCCTAAC 
ATGGATGGTT TCCATAACAC CCTTGTTGGT GGGATGTTTT TTGGGTTAGG TCGGAGGAAT 
CATAAAGTGG ATGGTGAGAA TTCTTCTAAG ATCACAAGTG ATCCTTATGT TACTGTCTCT 
ATCTCTGGTG CTGTGATTGG TAGAACTTTT GTTATTAGCA ATAGTGAGAA TCCTGTGTGG 
ATGCAGCATT TTGATGTACC AGTTGCTCAT AGTGCTGCGA AAGTTCACTT TGTTGTGAAA 
GACAGTGATA TTATTGGATC ACAGATCATT GGAGCTGTTG AAATCCCAAC CGAGCAGTTG 
TGTTCCGGGA ATAGAATCGA AGGGCTGTTT CCGATACTTA ACAGTAGAGG AAAGCCTTGT 
AAACAAGGAG TTCCCGGTAC ATACTTCCCT TTAAGGAAAG GCGGTAGGGT TACTCTTTAT 
CAGGATGCTC ATGTTGATGA CGGTACTCTT CCGAGTGTAC ATCTTGATGG TGGGATTCAG 
TATAGACATG GAAAATGCTG GGAAGATATG GCTGATGCGA TACGACGGGC AAGGAGGTTG 
ATTTATATCA CAGGTTGGTC AGTTTTCCAT CCGGTTAGGC TGGTTCGTCG TAACAATGAT 
CCGACCCAAG GTACATTAGG AGAGTTGCTT AAAGTCAAAT CTCAAGAAGG TGTTAGAGTG 
TTGGTTTTGG TGTGGGATGA TCCAACTTCA AGGAGTCTTT TGGGATTCAG TACAAAAGGA 
CTTATGAACA CAAGCGATGA GGAGACTCGC CGTTTTTTCA AGCACTCATC GGTGCAAGTT 
CTCCTTTGTC CTAGATATGG AGGGAAAGGT CACAGCTTCA TAAAAAAATC TGAAGTTGAA 
ACAATCTACA CGCATCATCA GAAAACTATG ATTGTAGATG CTGAAGCAGC TCAGAACCGA 
AGAAAGATTG TAGCATTTGT TGGAGGGCTA GACTTGTGCA ACGGACGATT TGATACTCCC 
AAGCATCCTC TCTTCAGGAC ATTAAAGACA ATCCATAAAG ATGACTTCCA TAACCCAAAT 
TTTGTGACTA CTGCGGATGA TGGACCAAGA GAACCATGGC ATGATCTGCA CAGCAAGATT 
GATGGTCCAG CTGCATATGA TGTGCTTGCT AATTTTGAGG AACGCTGGAT GAAGGCTTCA 
AAACCTCGCG GAATTGGGAG ACTAAGAACT TCTTCTGATG ATTCTTTGCT TAGGTTAGAC 
AGAATTCCTG ACATAATGGG ACTTTCCGAA GCTTCTTCTG CTAATGATAA TGATCCAGAG 
TCTTGGCATG TTCAGGTTTT CCGTTCAATT GATTCGAGTT CAGTTAAAGG GTTTCCAAAG 
GACCCAAAGG AAGCTACTGG AAGAAACCTT CTATGTGGGA AGAACATTCT CATAGATATG 
AGCATACATG CAGCTTATGT TAAAGCCATA CGATCTGCTC AGCACTTCAT TTACATTGAA 
AACCAATATT TTCTTGGATC ATCATTCAAT TGGGATTCAA ACAAGAACTT GGGTGCGAAT 
AATCTAATCC CGATGGAAAT CGCGCTGAAG ATTGCTAACA AAATTAGAGC AAGGGAAAAG 
TTTGCTGCTT ATATTGTCAT TCCAATGTGG CCAGAAGGTG CTCCAACAAG TAATCCTATT 
CAAAGAATTC TCTACTGGCA GCACAAAACC ATGCAAATGA TGTATCAAAC CATCTACAAG 
GCACTTGTGG AAGTTGGTCT TGATGGCCAG CTTGAGCCAC AAGACTTTTT GAACTTTTTC 
TGTCTTGGAA CCAGAGAGGT TGGAACCAGA GAGGTTCCTG ATGGAACTGT TAGCGTATAT 
AATAGTCCTC GGAAGCCACC TCAGCTAAAT GCTGCACAGG TACAAGCTTT GAAGAGTCGG 
AGATTCATGA TATATGTTCA TTCCAAAGGT ATGGTAGTGG ATGATGAGTT TGTCTTAATT 
GGTTCCGCGA ATATCAACCA GAGATCCTTG GAAGGAACTA GAGACACTGA AATCGCTATG 
GGAGGGTATC AGCCACACCA TTCATGGGCT AAGAAAGGTT CTCGTCCTCG TGGTCAGATC 
TTTGGATATA GAATGTCGTT ATGGGCAGAA CATCTAGGGT TTCTAGAGCA AGAATTCGAG 
GAACCAGAGA ACATGGAATG CGTGAGACGA GTTAGGCAAT TGAGTGAGCT GAATTGGAGA 
CAATATGCAG CGGAAGAAGT CACAGAGATG CCAGGTCATC TTCTTAAGTA TCCAGTTCAA 
GTTGATAGAA CAGGCAAAGT GAGTTCTCTT CCTGGATACG AGACATTCCC AGATCTCGGT 
GGCAAGATCA TAGGATCGTT CCTTGTAGTA GAAGAAAACC TCACCATATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001329644.1
CDS	436..2946
Translation	MAYHPVYNETMSMGGGSSNEFGQWLDKQLVPFDTSSGSLRVELLHGNLDIWVKEAKHLPNMDGFHNTLVGGMFFGLGRRNHKVDGENSSKITSDPYVTVSISGAVIGRTFVISNSENPVWMQHFDVPVAHSAAKVHFVVKDSDIIGSQIIGAVEIPTEQLCSGNRIEGLFPILNSRGKPCKQGVPGTYFPLRKGGRVTLYQDAHVDDGTLPSVHLDGGIQYRHGKCWEDMADAIRRARRLIYITGWSVFHPVRLVRRNNDPTQGTLGELLKVKSQEGVRVLVLVWDDPTSRSLLGFSTKGLMNTSDEETRRFFKHSSVQVLLCPRYGGKGHSFIKKSEVETIYTHHQKTMIVDAEAAQNRRKIVAFVGGLDLCNGRFDTPKHPLFRTLKTIHKDDFHNPNFVTTADDGPREPWHDLHSKIDGPAAYDVLANFEERWMKASKPRGIGRLRTSSDDSLLRLDRIPDIMGLSEASSANDNDPESWHVQVFRSIDSSSVKGFPKDPKEATGRNLLCGKNILIDMSIHAAYVKAIRSAQHFIYIENQYFLGSSFNWDSNKNLGANNLIPMEIALKIANKIRAREKFAAYIVIPMWPEGAPTSNPIQRILYWQHKTMQMMYQTIYKALVEVGLDGQLEPQDFLNFFCLGTREVGTREVPDGTVSVYNSPRKPPQLNAAQVQALKSRRFMIYVHSKGMVVDDEFVLIGSANINQRSLEGTRDTEIAMGGYQPHHSWAKKGSRPRGQIFGYRMSLWAEHLGFLEQEFEEPENMECVRRVRQLSELNWRQYAAEEVTEMPGHLLKYPVQVDRTGKVSSLPGYETFPDLGGKIIGSFLVVEENLTI

Target ORF information:

RefSeq Version	NM_001340738.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana phospholipase D gamma 3 (PLDGAMMA3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001340738.1

ORF Insert Sequence:

ATGGCGTATC ATCCAGTTTA TAACGAGACT ATGTCTATGG GAGGAGGGTC AAGCAACGAG 
TTTGGCCAGT GGCTTGACAA GCAACTCGTG CCGTTTGATA CAAGTAGTGG CTCTTTGAGG 
GTTGAATTAT TACATGGTAA CTTAGACATT TGGGTTAAGG AAGCTAAACA TCTTCCTAAC 
ATGGATGGTT TCCATAACAC CCTTGTTGGT GGGATGTTTT TTGGGTTAGG TCGGAGGAAT 
CATAAAGTGG ATGGTGAGAA TTCTTCTAAG ATCACAAGTG ATCCTTATGT TACTGTCTCT 
ATCTCTGGTG CTGTGATTGG TAGAACTTTT GTTATTAGCA ATAGTGAGAA TCCTGTGTGG 
ATGCAGCATT TTGATGTACC AGTTGCTCAT AGTGCTGCGA AAGTTCACTT TGTTGTGAAA 
GACAGTGATA TTATTGGATC ACAGATCATT GGAGCTGTTG AAATCCCAAC CGAGCAGTTG 
TGTTCCGGGA ATAGAATCGA AGGGCTGTTT CCGATACTTA ACAGTAGAGG AAAGCCTTGT 
AAACAAGGAG TTCCCGGTAC ATACTTCCCT TTAAGGAAAG GCGGTAGGGT TACTCTTTAT 
CAGGATGCTC ATGTTGATGA CGGTACTCTT CCGAGTGTAC ATCTTGATGG TGGGATTCAG 
TATAGACATG GAAAATGCTG GGAAGATATG GCTGATGCGA TACGACGGGC AAGGAGGTTG 
ATTTATATCA CAGGTTGGTC AGTTTTCCAT CCGGTTAGGC TGGTTCGTCG TAACAATGAT 
CCGACCCAAG GTACATTAGG AGAGTTGCTT AAAGTCAAAT CTCAAGAAGG TGTTAGAGTG 
TTGGTTTTGG TGTGGGATGA TCCAACTTCA AGGAGTCTTT TGGGATTCAG TACAAAAGGA 
CTTATGAACA CAAGCGATGA GGAGACTCGC CGTTTTTTCA AGCACTCATC GGTGCAAGTT 
CTCCTTTGTC CTAGATATGG AGGGAAAGGT CACAGCTTCA TAAAAAAATC TGAAGTTGAA 
ACAATCTACA CGCATCATCA GAAAACTATG ATTGTAGATG CTGAAGCAGC TCAGAACCGA 
AGAAAGATTG TAGCATTTGT TGGAGGGCTA GACTTGTGCA ACGGACGATT TGATACTCCC 
AAGCATCCTC TCTTCAGGAC ATTAAAGACA ATCCATAAAG ATGACTTCCA TAACCCAAAT 
TTTGTGACTA CTGCGGATGA TGGACCAAGA GAACCATGGC ATGATCTGCA CAGCAAGATT 
GATGGTCCAG CTGCATATGA TGTGCTTGCT AATTTTGAGG AACGCTGGAT GAAGGCTTCA 
AAACCTCGCG GAATTGGGAG ACTAAGAACT TCTTCTGATG ATTCTTTGCT TAGGTTAGAC 
AGAATTCCTG ACATAATGGG ACTTTCCGAA GCTTCTTCTG CTAATGATAA TGATCCAGAG 
TCTTGGCATG TTCAGGTTTT CCGTTCAATT GATTCGAGTT CAGTTAAAGG GTTTCCAAAG 
GACCCAAAGG AAGCTACTGG AAGAAACCTT CTATGTGGGA AGAACATTCT CATAGATATG 
AGCATACATG CAGCTTATGT TAAAGCCATA CGATCTGCTC AGCACTTCAT TTACATTGAA 
AACCAATATT TTCTTGGATC ATCATTCAAT TGGGATTCAA ACAAGAACTT GGGTGCGAAT 
AATCTAATCC CGATGGAAAT CGCGCTGAAG ATTGCTAACA AAATTAGAGC AAGGGAAAAG 
TTTGCTGCTT ATATTGTCAT TCCAATGTGG CCAGAAGGTG CTCCAACAAG TAATCCTATT 
CAAAGAATTC TCTACTGGCA GCACAAAACC ATGCAAATGA TGTATCAAAC CATCTACAAG 
GCACTTGTGG AAGTTGGTCT TGATGGCCAG CTTGAGCCAC AAGACTTTTT GAACTTTTTC 
TGTCTTGGAA CCAGAGAGGT TGGAACCAGA GAGGTTCCTG ATGGAACTGT TAGCGTATAT 
AATAGTCCTC GGAAGCCACC TCAGCTAAAT GCTGCACAGG TACAAGCTTT GAAGAGTCGG 
AGATTCATGA TATATGTTCA TTCCAAAGGT ATGGTAGTGG ATGATGAGTT TGTCTTAATT 
GGTTCCGCGA ATATCAACCA GAGATCCTTG GAAGGAACTA GAGACACTGA AATCGCTATG 
GGAGGGTATC AGCCACACCA TTCATGGGCT AAGAAAGGTT CTCGTCCTCG TGGTCAGATC 
TTTGGATATA GAATGTCGTT ATGGGCAGAA CATCTAGGGT TTCTAGAGCA AGAATTCGAG 
GAACCAGAGA ACATGGAATG CGTGAGACGA GTTAGGCAAT TGAGTGAGCT GAATTGGAGA 
CAATATGCAG CGGAAGAAGT CACAGAGATG CCAGGTCATC TTCTTAAGTA TCCAGTTCAA 
GTTGATAGAA CAGGCAAAGT GAGTTCTCTT CCTGGATACG AGACATTCCC AGATCTCGGT 
GGCAAGATCA TAGGATCGTT CCTTGTAGTA GAAGAAAACC TCACCATATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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PLDGAMMA3 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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