TKL cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	TKL
Entrez Gene ID	825493
Full Name	TIC-like protein
Synonyms	ATTKL,TIC-like,TICKLE
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001084865.2	NP_001078334.1	TIC-like protein
NM_116183.4	NP_191877.2	TIC-like protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Salanoubat M, Lemcke K, Rieger M, Ansorge W, Unseld M, Fartmann B, Valle G, Bl?cker H, Perez-Alonso M, Obermaier B, Delseny M, Boutry M, Grivell LA, Mache R, Puigdom?nech P, De Simone V, Choisne N, Artiguenave F, Robert C, Brottier P, Wincker P, Cattolico L, Weissenbach J, Saurin W, Qu?tier F, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Benes V, Wurmbach E, Drzonek H, Erfle H, Jordan N, Bangert S, Wiedelmann R, Kranz H, Voss H, Holland R, Brandt P, Nyakatura G, Vezzi A, D'Angelo M, Pallavicini A, Toppo S, Simionati B, Conrad A, Hornischer K, Kauer G, L?hnert TH, Nordsiek G, Reichelt J, Scharfe M, Sch?n O, Bargues M, Terol J, Climent J, Navarro P, Collado C, Perez-Perez A, Ottenw?lder B, Duchemin D, Cooke R, Laudie M, Berger-Llauro C, Purnelle B, Masuy D, de Haan M, Maarse AC, Alcaraz JP, Cottet A, Casacuberta E, Monfort A, Argiriou A, flores M, Liguori R, Vitale D, Mannhaupt G, Haase D, Schoof H, Rudd S, Zaccaria P, Mewes HW, Mayer KF, Kaul S, Town CD, Koo HL, Tallon LJ, Jenkins J, Rooney T, Rizzo M, Walts A, Utterback T, Fujii CY, Shea TP, Creasy TH, Haas B, Maiti R, Wu D, Peterson J, Van Aken S, Pai G, Militscher J, Sellers P, Gill JE, Feldblyum TV, Preuss D, Lin X, Nierman WC, Salzberg SL, White O, Venter JC, Fraser CM, Kaneko T, Nakamura Y, Sato S, Kato T, Asamizu E, Sasamoto S, Kimura T, Idesawa K, Kawashima K, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S, , ,
Nature408(6814)820-2(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TKL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TKL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb14200	NM_001084865.2 Latest version!	Arabidopsis thaliana TIC-like protein (TKL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb14201	NM_116183.4 Latest version!	Arabidopsis thaliana TIC-like protein (TKL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TKL ( NM_001084865.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001078334.1 Arabidopsis thaliana TIC-like protein (TKL), mRNA.

Price & Availability↑

CloneID	OAb14200
Clone ID Related Accession (Same CDS sequence)	NM_001084865.2
Accession Version	NM_001084865.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	TIC-like protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Apr 24, 2008 this sequence version replaced NM_001084865.1.

ATGGATAAAC CCAGGGAAGC TCGAAGATCA ACGACAAACA TGGCTGCATC AAACGGAGTT 
ACTAAACGGA GGCAGAGAAT CTCAGAAAAC AGTCGTGAGC AGATGCATCA GGAGGTGGCG 
ACGGTGAGAG ATCGACCGGG GAAGAAAGAG CGAGATCCGG AATCGTTCAA TCGGAGTAAG 
AGGAGAAGAA GCGAGAGGTT CGCGCCGAGG AACGAGAGAG ATGTGGAAGA AGATAGCTCG 
GATGAGAGTC AAGGAGACGA CGATTATCAT CAGAGGAAGA GCTTCTCGTC GGGTCGAATC 
CCCAGGCATG CGGCGTCTTT GATGGTCGCC GATGAAATGA TCGGCGTACC TGTCCCGAGA 
AAGGCTCGCT CAGCATGTTT AAAGAGGTCG CATGACTGCC GGACTTCGTC GGGAAGCCAT 
AGCTGCGAAG TCGTTTCACC GTCTTCTTCA ACTCTTTCCG TCAAAGAGAG AAAGATTGTA 
AACGGATCCA AATCCCGGAT ACCCAAACCA CCGAAATCCT CCGGCACGGT TGAGGACGAC 
TTGGAGATCG AAATAGCAGA GGTTCTCTCC GGATTGAAAA AGCAGCCTCA TAGCTCAAAG 
AGAGGAGACG ATTCGGAGAA TTCTCAAAAA TCATCGGAGG TTAAAGGTAT TAAGCAGTTA 
CCTGCAGGTG TAGAGATTTG CGGTTTATTC ACTTGTAAAG CGAGATTCGA CTCAGCCAAC 
ATTGATAAAT CGACCAACGG AGCTAAAACT TCGTTAGTAG TCGCCTCAGA TATTGTGGAT 
GAGAAAGAAC CAAGGGTATC AGAGGGTCCA CAATTGGAAA ACAACAAAGA TGAGAGTCAT 
TGTATTAGTA AGCTCGATAT TCATCTCATG GTTCCTCCAA CATTGCCTTC CTCTTCAGGG 
AGAGTTTCTC TTCTCCCACT CGTTTCTGAT TATTGCAAGC GAGATAGTGC TCTAAAGAGC 
AAAGAGAAGA TTACTGTCAA GAATGAGATG AATCCAGAAG AAGTCAAACA AAAGAAGGTT 
GATAAACGTT ACTGGCTGAA TCTTGATATA GAGGGGCCGA ATCAAGAGAC CGACAGAGAC 
TCTAATTTAA GATTACAGAA TTTGGATTGG AGCCAACCTC AGCAGGCTAA ATCTGCTCCG 
CACAGTTCAG TCTTACCTTT GCCAGTAGCT GTAGCCAGCT GGCCTAGCGG GGTTCCTCCT 
CAGGGACATG TAGCCCTGAT CCAGACCGGT AAACCTGTGG ATGAAAGCAA TGGATCTTCT 
AAATTAGTGC AGCCTAGGCC TAAAAGGTGT GCCACACACT TTTTCATAGC TCGGAACATT 
CAGCTACATC AGCATTTCCT CAAAACAAAC CATCTCCCTA CACCAAACAA AGGTTCTGTT 
TATCTGAAAG GTGGTGACTT GAGGCCAACC GCTGGAAACC CATCCTTGCA TGGAAGCTCT 
CCTATTCTCA GTTTAAACTC CCAACCACAC GTTAGAAACG GAGATAATAT TTCTGCGCCT 
AATGTTAAAG CCTCTGAAAG TGGTCATTTT GCCTCAACCA GACAGAATAA ACCTCAACCA 
CCCCCTGCAT CAATTAGTGT AGTGCCTGCT CCAGCTTTTA TCTACCCCGC AAACCACCAT 
TTGCAACCAG TCATGGTACC TTCTAAATCA TCTCGCCCAA CCAAGAGCCC CCATTTGGCT 
GTTGGTTTAG CATCTGCTAA CTTCAGCCAT CCAAGTTCAT CAGCCAGCGA AGTTTCTTCT 
CCATACTTAA CAGTTATTCC AAACAATGCA TATTCCTTTC AACTGTCATC AACCATCAGA 
GGTGGGACTC CAAGCCAGGC GGTGCCTTTC TACAATGGGT CTTTTTACTC GCCTCAGATG 
TTCCAACAAC CTCCTCAGCC TCTGCAGAGA CAGAGTCAAG CCCAGAGGGA GTCAAAGGCA 
TCAAGCTGTT CCTCATCTTC CCACAGGCAA CCGCAAGTTA GTGTGAATAG TCTTTCAAGC 
CAAGCTAATG TTCAGCAACA CCGTCAAATG TCACAAAAGT TTGAGGTAGC TGGAGTTAAC 
ACTGATTCAA GGGGTAGCCA CACTCAGAAA GGAGGCCCAT TTGGCCAGAT CATGGCAGCC 
CCTGTTCAGC CACAAAACTT TTCCATGTCG TTTGCGTCTA TCGCCAGCAG TGCTCCCCCT 
GCAACTCTGA ATTTTTCCTC CAATGGCTAT CACATCTCTA CACCTCCTGG TGTTGCTCAC 
CAGAAAAACC ATCAATCAAG TGAGGCAAAG ACTGGAGGTG GAAGCTGTTC AAGCAATGCC 
GAAGACCCGA AAAAGAATCT TCAAGGGAAG CCTCAAGGGA TGATGAATGG ACATACGCTG 
GTTTTTGACA ATCCATCGAG AACCCTCAAC TTTGTTTCTG GTACTTGGCC TCCTCCTGCA 
GCAACAGCAA TAAACGGAGA CCCTTCAGTG TTTACGCAGC ATCTCACGCA GAGGCAGCAG 
CAATCTGGTC GGAGTAAAAT GATGATGACT CATTCGCAGG CTGACTCTGT TTCTGCCACG 
TCATCCCAGT GGAAAAACCC TGCAACCTCA TCATCACTCA CGTCGTGTAC TTCCTTAAAC 
CTCAAGCAGT TCCAGTCGCA GCAGCAGATC AGAACACACG GACAGACACA GATTTCTTTT 
GCAGCACCAA CAAATCCGCA GCCTTCTCAG GGGAAACAGG GGAGATCTGG TGGCTCTTCT 
CCTTCAGTCA CTGGGTCTGC TTCACATGGC AAACCAGCGA ACTCTAAGGT CAGCAATAGC 
AAAGCCTTGC TATTATCACC TGTTCCTTTG TCACAGGAAC ACACAGAGAA TCCAGCTTCC 
GGGTCGACCC AAAAAAACTC TCCCGTTTGT GGAAGGACCG TGCCACCGAT TATAACTTCG 
TGCCCTGGTC ATCTGTCCGA GCTTAAGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001078334.1
CDS	157..3069
Translation	MDKPREARRSTTNMAASNGVTKRRQRISENSREQMHQEVATVRDRPGKKERDPESFNRSKRRRSERFAPRNERDVEEDSSDESQGDDDYHQRKSFSSGRIPRHAASLMVADEMIGVPVPRKARSACLKRSHDCRTSSGSHSCEVVSPSSSTLSVKERKIVNGSKSRIPKPPKSSGTVEDDLEIEIAEVLSGLKKQPHSSKRGDDSENSQKSSEVKGIKQLPAGVEICGLFTCKARFDSANIDKSTNGAKTSLVVASDIVDEKEPRVSEGPQLENNKDESHCISKLDIHLMVPPTLPSSSGRVSLLPLVSDYCKRDSALKSKEKITVKNEMNPEEVKQKKVDKRYWLNLDIEGPNQETDRDSNLRLQNLDWSQPQQAKSAPHSSVLPLPVAVASWPSGVPPQGHVALIQTGKPVDESNGSSKLVQPRPKRCATHFFIARNIQLHQHFLKTNHLPTPNKGSVYLKGGDLRPTAGNPSLHGSSPILSLNSQPHVRNGDNISAPNVKASESGHFASTRQNKPQPPPASISVVPAPAFIYPANHHLQPVMVPSKSSRPTKSPHLAVGLASANFSHPSSSASEVSSPYLTVIPNNAYSFQLSSTIRGGTPSQAVPFYNGSFYSPQMFQQPPQPLQRQSQAQRESKASSCSSSSHRQPQVSVNSLSSQANVQQHRQMSQKFEVAGVNTDSRGSHTQKGGPFGQIMAAPVQPQNFSMSFASIASSAPPATLNFSSNGYHISTPPGVAHQKNHQSSEAKTGGGSCSSNAEDPKKNLQGKPQGMMNGHTLVFDNPSRTLNFVSGTWPPPAATAINGDPSVFTQHLTQRQQQSGRSKMMMTHSQADSVSATSSQWKNPATSSSLTSCTSLNLKQFQSQQQIRTHGQTQISFAAPTNPQPSQGKQGRSGGSSPSVTGSASHGKPANSKVSNSKALLLSPVPLSQEHTENPASGSTQKNSPVCGRTVPPIITSCPGHLSELKY

Target ORF information:

RefSeq Version	NM_001084865.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana TIC-like protein (TKL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001084865.2

ORF Insert Sequence:

ATGGATAAAC CCAGGGAAGC TCGAAGATCA ACGACAAACA TGGCTGCATC AAACGGAGTT 
ACTAAACGGA GGCAGAGAAT CTCAGAAAAC AGTCGTGAGC AGATGCATCA GGAGGTGGCG 
ACGGTGAGAG ATCGACCGGG GAAGAAAGAG CGAGATCCGG AATCGTTCAA TCGGAGTAAG 
AGGAGAAGAA GCGAGAGGTT CGCGCCGAGG AACGAGAGAG ATGTGGAAGA AGATAGCTCG 
GATGAGAGTC AAGGAGACGA CGATTATCAT CAGAGGAAGA GCTTCTCGTC GGGTCGAATC 
CCCAGGCATG CGGCGTCTTT GATGGTCGCC GATGAAATGA TCGGCGTACC TGTCCCGAGA 
AAGGCTCGCT CAGCATGTTT AAAGAGGTCG CATGACTGCC GGACTTCGTC GGGAAGCCAT 
AGCTGCGAAG TCGTTTCACC GTCTTCTTCA ACTCTTTCCG TCAAAGAGAG AAAGATTGTA 
AACGGATCCA AATCCCGGAT ACCCAAACCA CCGAAATCCT CCGGCACGGT TGAGGACGAC 
TTGGAGATCG AAATAGCAGA GGTTCTCTCC GGATTGAAAA AGCAGCCTCA TAGCTCAAAG 
AGAGGAGACG ATTCGGAGAA TTCTCAAAAA TCATCGGAGG TTAAAGGTAT TAAGCAGTTA 
CCTGCAGGTG TAGAGATTTG CGGTTTATTC ACTTGTAAAG CGAGATTCGA CTCAGCCAAC 
ATTGATAAAT CGACCAACGG AGCTAAAACT TCGTTAGTAG TCGCCTCAGA TATTGTGGAT 
GAGAAAGAAC CAAGGGTATC AGAGGGTCCA CAATTGGAAA ACAACAAAGA TGAGAGTCAT 
TGTATTAGTA AGCTCGATAT TCATCTCATG GTTCCTCCAA CATTGCCTTC CTCTTCAGGG 
AGAGTTTCTC TTCTCCCACT CGTTTCTGAT TATTGCAAGC GAGATAGTGC TCTAAAGAGC 
AAAGAGAAGA TTACTGTCAA GAATGAGATG AATCCAGAAG AAGTCAAACA AAAGAAGGTT 
GATAAACGTT ACTGGCTGAA TCTTGATATA GAGGGGCCGA ATCAAGAGAC CGACAGAGAC 
TCTAATTTAA GATTACAGAA TTTGGATTGG AGCCAACCTC AGCAGGCTAA ATCTGCTCCG 
CACAGTTCAG TCTTACCTTT GCCAGTAGCT GTAGCCAGCT GGCCTAGCGG GGTTCCTCCT 
CAGGGACATG TAGCCCTGAT CCAGACCGGT AAACCTGTGG ATGAAAGCAA TGGATCTTCT 
AAATTAGTGC AGCCTAGGCC TAAAAGGTGT GCCACACACT TTTTCATAGC TCGGAACATT 
CAGCTACATC AGCATTTCCT CAAAACAAAC CATCTCCCTA CACCAAACAA AGGTTCTGTT 
TATCTGAAAG GTGGTGACTT GAGGCCAACC GCTGGAAACC CATCCTTGCA TGGAAGCTCT 
CCTATTCTCA GTTTAAACTC CCAACCACAC GTTAGAAACG GAGATAATAT TTCTGCGCCT 
AATGTTAAAG CCTCTGAAAG TGGTCATTTT GCCTCAACCA GACAGAATAA ACCTCAACCA 
CCCCCTGCAT CAATTAGTGT AGTGCCTGCT CCAGCTTTTA TCTACCCCGC AAACCACCAT 
TTGCAACCAG TCATGGTACC TTCTAAATCA TCTCGCCCAA CCAAGAGCCC CCATTTGGCT 
GTTGGTTTAG CATCTGCTAA CTTCAGCCAT CCAAGTTCAT CAGCCAGCGA AGTTTCTTCT 
CCATACTTAA CAGTTATTCC AAACAATGCA TATTCCTTTC AACTGTCATC AACCATCAGA 
GGTGGGACTC CAAGCCAGGC GGTGCCTTTC TACAATGGGT CTTTTTACTC GCCTCAGATG 
TTCCAACAAC CTCCTCAGCC TCTGCAGAGA CAGAGTCAAG CCCAGAGGGA GTCAAAGGCA 
TCAAGCTGTT CCTCATCTTC CCACAGGCAA CCGCAAGTTA GTGTGAATAG TCTTTCAAGC 
CAAGCTAATG TTCAGCAACA CCGTCAAATG TCACAAAAGT TTGAGGTAGC TGGAGTTAAC 
ACTGATTCAA GGGGTAGCCA CACTCAGAAA GGAGGCCCAT TTGGCCAGAT CATGGCAGCC 
CCTGTTCAGC CACAAAACTT TTCCATGTCG TTTGCGTCTA TCGCCAGCAG TGCTCCCCCT 
GCAACTCTGA ATTTTTCCTC CAATGGCTAT CACATCTCTA CACCTCCTGG TGTTGCTCAC 
CAGAAAAACC ATCAATCAAG TGAGGCAAAG ACTGGAGGTG GAAGCTGTTC AAGCAATGCC 
GAAGACCCGA AAAAGAATCT TCAAGGGAAG CCTCAAGGGA TGATGAATGG ACATACGCTG 
GTTTTTGACA ATCCATCGAG AACCCTCAAC TTTGTTTCTG GTACTTGGCC TCCTCCTGCA 
GCAACAGCAA TAAACGGAGA CCCTTCAGTG TTTACGCAGC ATCTCACGCA GAGGCAGCAG 
CAATCTGGTC GGAGTAAAAT GATGATGACT CATTCGCAGG CTGACTCTGT TTCTGCCACG 
TCATCCCAGT GGAAAAACCC TGCAACCTCA TCATCACTCA CGTCGTGTAC TTCCTTAAAC 
CTCAAGCAGT TCCAGTCGCA GCAGCAGATC AGAACACACG GACAGACACA GATTTCTTTT 
GCAGCACCAA CAAATCCGCA GCCTTCTCAG GGGAAACAGG GGAGATCTGG TGGCTCTTCT 
CCTTCAGTCA CTGGGTCTGC TTCACATGGC AAACCAGCGA ACTCTAAGGT CAGCAATAGC 
AAAGCCTTGC TATTATCACC TGTTCCTTTG TCACAGGAAC ACACAGAGAA TCCAGCTTCC 
GGGTCGACCC AAAAAAACTC TCCCGTTTGT GGAAGGACCG TGCCACCGAT TATAACTTCG 
TGCCCTGGTC ATCTGTCCGA GCTTAAGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TKL ( NM_116183.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_191877.2 Arabidopsis thaliana TIC-like protein (TKL), mRNA.

Price & Availability↑

CloneID	OAb14201
Clone ID Related Accession (Same CDS sequence)	NM_116183.4
Accession Version	NM_116183.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	TIC-like protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Sep 12, 2016 this sequence version replaced NM_116183.3.

ATGGATAAAC CCAGGGAAGC TCGAAGATCA ACGACAAACA TGGCTGCATC AAACGGAGTT 
ACTAAACGGA GGCAGAGAAT CTCAGAAAAC AGTCGTGAGC AGATGCATCA GGAGGTGGCG 
ACGGTGAGAG ATCGACCGGG GAAGAAAGAG CGAGATCCGG AATCGTTCAA TCGGAGTAAG 
AGGAGAAGAA GCGAGAGGTT CGCGCCGAGG AACGAGAGAG ATGTGGAAGA AGATAGCTCG 
GATGAGAGTC AAGGAGACGA CGATTATCAT CAGAGGAAGA GCTTCTCGTC GGGTCGAATC 
CCCAGGCATG CGGCGTCTTT GATGGTCGCC GATGAAATGA TCGGCGTACC TGTCCCGAGA 
AAGGCTCGCT CAGCATGTTT AAAGAGGTCG CATGACTGCC GGACTTCGTC GGGAAGCCAT 
AGCTGCGAAG TCGTTTCACC GTCTTCTTCA ACTCTTTCCG TCAAAGAGAG AAAGATTGTA 
AACGGATCCA AATCCCGGAT ACCCAAACCA CCGAAATCCT CCGGCACGGT TGAGGACGAC 
TTGGAGATCG AAATAGCAGA GGTTCTCTCC GGATTGAAAA AGCAGCCTCA TAGCTCAAAG 
AGAGGAGACG ATTCGGAGAA TTCTCAAAAA TCATCGGAGG TTAAAGGTAT TAAGCAGTTA 
CCTGCAGGTG TAGAGATTTG CGGTTTATTC ACTTGTAAAG CGAGATTCGA CTCAGCCAAC 
ATTGATAAAT CGACCAACGG AGCTAAAACT TCGTTAGTAG TCGCCTCAGA TATTGTGGAT 
GAGAAAGAAC CAAGGGTATC AGAGGGTCCA CAATTGGAAA ACAACAAAGA TGAGAGTCAT 
TGTATTAGTA AGCTCGATAT TCATCTCATG GTTCCTCCAA CATTGCCTTC CTCTTCAGGG 
AGAGTTTCTC TTCTCCCACT CGTTTCTGAT TATTGCAAGC GAGATAGTGC TCTAAAGAGC 
AAAGAGAAGA TTACTGTCAA GAATGAGATG AATCCAGAAG AAGTCAAACA AAAGAAGGTT 
GATAAACGTT ACTGGCTGAA TCTTGATATA GAGGGGCCGA ATCAAGAGAC CGACAGAGAC 
TCTAATTTAA GATTACAGAA TTTGGATTGG AGCCAACCTC AGCAGGCTAA ATCTGCTCCG 
CACAGTTCAG TCTTACCTTT GCCAGTAGCT GTAGCCAGCT GGCCTAGCGG GGTTCCTCCT 
CAGGGACATG TAGCCCTGAT CCAGACCGGT AAACCTGTGG ATGAAAGCAA TGGATCTTCT 
AAATTAGTGC AGGGTGCTCC AATTTCTGCC TCTCAGCCTA GGCCTAAAAG GTGTGCCACA 
CACTTTTTCA TAGCTCGGAA CATTCAGCTA CATCAGCATT TCCTCAAAAC AAACCATCTC 
CCTACACCAA ACAAAGGTTC TGTTTATCTG AAAGGTGGTG ACTTGAGGCC AACCGCTGGA 
AACCCATCCT TGCATGGAAG CTCTCCTATT CTCAGTTTAA ACTCCCAACC ACACGTTAGA 
AACGGAGATA ATATTTCTGC GCCTAATGTT AAAGCCTCTG AAAGTGGTCA TTTTGCCTCA 
ACCAGACAGA ATAAACCTCA ACCACCCCCT GCATCAATTA GTGTAGTGCC TGCTCCAGCT 
TTTATCTACC CCGCAAACCA CCATTTGCAA CCAGTCATGG TACCTTCTAA ATCATCTCGC 
CCAACCAAGA GCCCCCATTT GGCTGTTGGT TTAGCATCTG CTAACTTCAG CCATCCAAGT 
TCATCAGCCA GCGAAGTTTC TTCTCCATAC TTAACAGTTA TTCCAAACAA TGCATATTCC 
TTTCAACTGT CATCAACCAT CAGAGGTGGG ACTCCAAGCC AGGCGGTGCC TTTCTACAAT 
GGGTCTTTTT ACTCGCCTCA GATGTTCCAA CAACCTCCTC AGCCTCTGCA GAGACAGAGT 
CAAGCCCAGA GGGAGTCAAA GGCATCAAGC TGTTCCTCAT CTTCCCACAG GCAACCGCAA 
GTTAGTGTGA ATAGTCTTTC AAGCCAAGCT AATGTTCAGC AACACCGTCA AATGTCACAA 
AAGTTTGAGG TAGCTGGAGT TAACACTGAT TCAAGGGGTA GCCACACTCA GAAAGGAGGC 
CCATTTGGCC AGATCATGGC AGCCCCTGTT CAGCCACAAA ACTTTTCCAT GTCGTTTGCG 
TCTATCGCCA GCAGTGCTCC CCCTGCAACT CTGAATTTTT CCTCCAATGG CTATCACATC 
TCTACACCTC CTGGTGTTGC TCACCAGAAA AACCATCAAT CAAGTGAGGC AAAGACTGGA 
GGTGGAAGCT GTTCAAGCAA TGCCGAAGAC CCGAAAAAGA ATCTTCAAGG GAAGCCTCAA 
GGGATGATGA ATGGACATAC GCTGGTTTTT GACAATCCAT CGAGAACCCT CAACTTTGTT 
TCTGGTACTT GGCCTCCTCC TGCAGCAACA GCAATAAACG GAGACCCTTC AGTGTTTACG 
CAGCATCTCA CGCAGAGGCA GCAGCAATCT GGTCGGAGTA AAATGATGAT GACTCATTCG 
CAGGCTGACT CTGTTTCTGC CACGTCATCC CAGTGGAAAA ACCCTGCAAC CTCATCATCA 
CTCACGTCGT GTACTTCCTT AAACCTCAAG CAGTTCCAGT CGCAGCAGCA GATCAGAACA 
CACGGACAGA CACAGATTTC TTTTGCAGCA CCAACAAATC CGCAGCCTTC TCAGGGGAAA 
CAGGGGAGAT CTGGTGGCTC TTCTCCTTCA GTCACTGGGT CTGCTTCACA TGGCAAACCA 
GCGAACTCTA AGGTCAGCAA TAGCAAAGCC TTGCTATTAT CACCTGTTCC TTTGTCACAG 
GAACACACAG AGAATCCAGC TTCCGGGTCG ACCCAAAAAA ACTCTCCCGT TTGTGGAAGG 
ACCGTGCCAC CGATTATAAC TTCGTGCCCT GGTCATCTGT CCGAGCTTAA GTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_191877.2
CDS	180..3116
Translation	MDKPREARRSTTNMAASNGVTKRRQRISENSREQMHQEVATVRDRPGKKERDPESFNRSKRRRSERFAPRNERDVEEDSSDESQGDDDYHQRKSFSSGRIPRHAASLMVADEMIGVPVPRKARSACLKRSHDCRTSSGSHSCEVVSPSSSTLSVKERKIVNGSKSRIPKPPKSSGTVEDDLEIEIAEVLSGLKKQPHSSKRGDDSENSQKSSEVKGIKQLPAGVEICGLFTCKARFDSANIDKSTNGAKTSLVVASDIVDEKEPRVSEGPQLENNKDESHCISKLDIHLMVPPTLPSSSGRVSLLPLVSDYCKRDSALKSKEKITVKNEMNPEEVKQKKVDKRYWLNLDIEGPNQETDRDSNLRLQNLDWSQPQQAKSAPHSSVLPLPVAVASWPSGVPPQGHVALIQTGKPVDESNGSSKLVQGAPISASQPRPKRCATHFFIARNIQLHQHFLKTNHLPTPNKGSVYLKGGDLRPTAGNPSLHGSSPILSLNSQPHVRNGDNISAPNVKASESGHFASTRQNKPQPPPASISVVPAPAFIYPANHHLQPVMVPSKSSRPTKSPHLAVGLASANFSHPSSSASEVSSPYLTVIPNNAYSFQLSSTIRGGTPSQAVPFYNGSFYSPQMFQQPPQPLQRQSQAQRESKASSCSSSSHRQPQVSVNSLSSQANVQQHRQMSQKFEVAGVNTDSRGSHTQKGGPFGQIMAAPVQPQNFSMSFASIASSAPPATLNFSSNGYHISTPPGVAHQKNHQSSEAKTGGGSCSSNAEDPKKNLQGKPQGMMNGHTLVFDNPSRTLNFVSGTWPPPAATAINGDPSVFTQHLTQRQQQSGRSKMMMTHSQADSVSATSSQWKNPATSSSLTSCTSLNLKQFQSQQQIRTHGQTQISFAAPTNPQPSQGKQGRSGGSSPSVTGSASHGKPANSKVSNSKALLLSPVPLSQEHTENPASGSTQKNSPVCGRTVPPIITSCPGHLSELKY

Target ORF information:

RefSeq Version	NM_116183.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana TIC-like protein (TKL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_116183.4

ORF Insert Sequence:

ATGGATAAAC CCAGGGAAGC TCGAAGATCA ACGACAAACA TGGCTGCATC AAACGGAGTT 
ACTAAACGGA GGCAGAGAAT CTCAGAAAAC AGTCGTGAGC AGATGCATCA GGAGGTGGCG 
ACGGTGAGAG ATCGACCGGG GAAGAAAGAG CGAGATCCGG AATCGTTCAA TCGGAGTAAG 
AGGAGAAGAA GCGAGAGGTT CGCGCCGAGG AACGAGAGAG ATGTGGAAGA AGATAGCTCG 
GATGAGAGTC AAGGAGACGA CGATTATCAT CAGAGGAAGA GCTTCTCGTC GGGTCGAATC 
CCCAGGCATG CGGCGTCTTT GATGGTCGCC GATGAAATGA TCGGCGTACC TGTCCCGAGA 
AAGGCTCGCT CAGCATGTTT AAAGAGGTCG CATGACTGCC GGACTTCGTC GGGAAGCCAT 
AGCTGCGAAG TCGTTTCACC GTCTTCTTCA ACTCTTTCCG TCAAAGAGAG AAAGATTGTA 
AACGGATCCA AATCCCGGAT ACCCAAACCA CCGAAATCCT CCGGCACGGT TGAGGACGAC 
TTGGAGATCG AAATAGCAGA GGTTCTCTCC GGATTGAAAA AGCAGCCTCA TAGCTCAAAG 
AGAGGAGACG ATTCGGAGAA TTCTCAAAAA TCATCGGAGG TTAAAGGTAT TAAGCAGTTA 
CCTGCAGGTG TAGAGATTTG CGGTTTATTC ACTTGTAAAG CGAGATTCGA CTCAGCCAAC 
ATTGATAAAT CGACCAACGG AGCTAAAACT TCGTTAGTAG TCGCCTCAGA TATTGTGGAT 
GAGAAAGAAC CAAGGGTATC AGAGGGTCCA CAATTGGAAA ACAACAAAGA TGAGAGTCAT 
TGTATTAGTA AGCTCGATAT TCATCTCATG GTTCCTCCAA CATTGCCTTC CTCTTCAGGG 
AGAGTTTCTC TTCTCCCACT CGTTTCTGAT TATTGCAAGC GAGATAGTGC TCTAAAGAGC 
AAAGAGAAGA TTACTGTCAA GAATGAGATG AATCCAGAAG AAGTCAAACA AAAGAAGGTT 
GATAAACGTT ACTGGCTGAA TCTTGATATA GAGGGGCCGA ATCAAGAGAC CGACAGAGAC 
TCTAATTTAA GATTACAGAA TTTGGATTGG AGCCAACCTC AGCAGGCTAA ATCTGCTCCG 
CACAGTTCAG TCTTACCTTT GCCAGTAGCT GTAGCCAGCT GGCCTAGCGG GGTTCCTCCT 
CAGGGACATG TAGCCCTGAT CCAGACCGGT AAACCTGTGG ATGAAAGCAA TGGATCTTCT 
AAATTAGTGC AGGGTGCTCC AATTTCTGCC TCTCAGCCTA GGCCTAAAAG GTGTGCCACA 
CACTTTTTCA TAGCTCGGAA CATTCAGCTA CATCAGCATT TCCTCAAAAC AAACCATCTC 
CCTACACCAA ACAAAGGTTC TGTTTATCTG AAAGGTGGTG ACTTGAGGCC AACCGCTGGA 
AACCCATCCT TGCATGGAAG CTCTCCTATT CTCAGTTTAA ACTCCCAACC ACACGTTAGA 
AACGGAGATA ATATTTCTGC GCCTAATGTT AAAGCCTCTG AAAGTGGTCA TTTTGCCTCA 
ACCAGACAGA ATAAACCTCA ACCACCCCCT GCATCAATTA GTGTAGTGCC TGCTCCAGCT 
TTTATCTACC CCGCAAACCA CCATTTGCAA CCAGTCATGG TACCTTCTAA ATCATCTCGC 
CCAACCAAGA GCCCCCATTT GGCTGTTGGT TTAGCATCTG CTAACTTCAG CCATCCAAGT 
TCATCAGCCA GCGAAGTTTC TTCTCCATAC TTAACAGTTA TTCCAAACAA TGCATATTCC 
TTTCAACTGT CATCAACCAT CAGAGGTGGG ACTCCAAGCC AGGCGGTGCC TTTCTACAAT 
GGGTCTTTTT ACTCGCCTCA GATGTTCCAA CAACCTCCTC AGCCTCTGCA GAGACAGAGT 
CAAGCCCAGA GGGAGTCAAA GGCATCAAGC TGTTCCTCAT CTTCCCACAG GCAACCGCAA 
GTTAGTGTGA ATAGTCTTTC AAGCCAAGCT AATGTTCAGC AACACCGTCA AATGTCACAA 
AAGTTTGAGG TAGCTGGAGT TAACACTGAT TCAAGGGGTA GCCACACTCA GAAAGGAGGC 
CCATTTGGCC AGATCATGGC AGCCCCTGTT CAGCCACAAA ACTTTTCCAT GTCGTTTGCG 
TCTATCGCCA GCAGTGCTCC CCCTGCAACT CTGAATTTTT CCTCCAATGG CTATCACATC 
TCTACACCTC CTGGTGTTGC TCACCAGAAA AACCATCAAT CAAGTGAGGC AAAGACTGGA 
GGTGGAAGCT GTTCAAGCAA TGCCGAAGAC CCGAAAAAGA ATCTTCAAGG GAAGCCTCAA 
GGGATGATGA ATGGACATAC GCTGGTTTTT GACAATCCAT CGAGAACCCT CAACTTTGTT 
TCTGGTACTT GGCCTCCTCC TGCAGCAACA GCAATAAACG GAGACCCTTC AGTGTTTACG 
CAGCATCTCA CGCAGAGGCA GCAGCAATCT GGTCGGAGTA AAATGATGAT GACTCATTCG 
CAGGCTGACT CTGTTTCTGC CACGTCATCC CAGTGGAAAA ACCCTGCAAC CTCATCATCA 
CTCACGTCGT GTACTTCCTT AAACCTCAAG CAGTTCCAGT CGCAGCAGCA GATCAGAACA 
CACGGACAGA CACAGATTTC TTTTGCAGCA CCAACAAATC CGCAGCCTTC TCAGGGGAAA 
CAGGGGAGAT CTGGTGGCTC TTCTCCTTCA GTCACTGGGT CTGCTTCACA TGGCAAACCA 
GCGAACTCTA AGGTCAGCAA TAGCAAAGCC TTGCTATTAT CACCTGTTCC TTTGTCACAG 
GAACACACAG AGAATCCAGC TTCCGGGTCG ACCCAAAAAA ACTCTCCCGT TTGTGGAAGG 
ACCGTGCCAC CGATTATAAC TTCGTGCCCT GGTCATCTGT CCGAGCTTAA GTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Nature408(6814)820-2(2000 Dec)
Salanoubat M,Lemcke K,Rieger M,Ansorge W,Unseld M,Fartmann B,Valle G,Bl?cker H,Perez-Alonso M,Obermaier B,Delseny M,Boutry M,Grivell LA,Mache R,Puigdom?nech P,De Simone V,Choisne N,Artiguenave F,Robert C,Brottier P,Wincker P,Cattolico L,Weissenbach J,Saurin W,Qu?tier F,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Benes V,Wurmbach E,Drzonek H,Erfle H,Jordan N,Bangert S,Wiedelmann R,Kranz H,Voss H,Holland R,Brandt P,Nyakatura G,Vezzi A,D'Angelo M,Pallavicini A,Toppo S,Simionati B,Conrad A,Hornischer K,Kauer G,L?hnert TH,Nordsiek G,Reichelt J,Scharfe M,Sch?n O,Bargues M,Terol J,Climent J,Navarro P,Collado C,Perez-Perez A,Ottenw?lder B,Duchemin D,Cooke R,Laudie M,Berger-Llauro C,Purnelle B,Masuy D,de Haan M,Maarse AC,Alcaraz JP,Cottet A,Casacuberta E,Monfort A,Argiriou A,flores M,Liguori R,Vitale D,Mannhaupt G,Haase D,Schoof H,Rudd S,Zaccaria P,Mewes HW,Mayer KF,Kaul S,Town CD,Koo HL,Tallon LJ,Jenkins J,Rooney T,Rizzo M,Walts A,Utterback T,Fujii CY,Shea TP,Creasy TH,Haas B,Maiti R,Wu D,Peterson J,Van Aken S,Pai G,Militscher J,Sellers P,Gill JE,Feldblyum TV,Preuss D,Lin X,Nierman WC,Salzberg SL,White O,Venter JC,Fraser CM,Kaneko T,Nakamura Y,Sato S,Kato T,Asamizu E,Sasamoto S,Kimura T,Idesawa K,Kawashima K,Kishida Y,Kiyokawa C,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Tabata S,,,

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TKL cDNA ORF clone, Arabidopsis thaliana(thale cress)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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