ADF1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	ADF1
Entrez Gene ID	823744
Full Name	actin depolymerizing factor 1
Synonyms	ATADF1,actin depolymerizing factor 1,atadf
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001084778.1	NP_001078247.1	actin depolymerizing factor 1
NM_114470.3	NP_190187.1	actin depolymerizing factor 1

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	ADF4	NP_851228.1
Arabidopsis thaliana (thale cress)	ADF2	NP_566882.1
Arabidopsis thaliana (thale cress)	ADF3	NP_851227.1
Arabidopsis thaliana (thale cress)	ADF1	NP_190187.1
Oryza sativa (rice)	Os05g0113400	NP_001054456.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Salanoubat M, Lemcke K, Rieger M, Ansorge W, Unseld M, Fartmann B, Valle G, Bl?cker H, Perez-Alonso M, Obermaier B, Delseny M, Boutry M, Grivell LA, Mache R, Puigdom?nech P, De Simone V, Choisne N, Artiguenave F, Robert C, Brottier P, Wincker P, Cattolico L, Weissenbach J, Saurin W, Qu?tier F, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Benes V, Wurmbach E, Drzonek H, Erfle H, Jordan N, Bangert S, Wiedelmann R, Kranz H, Voss H, Holland R, Brandt P, Nyakatura G, Vezzi A, D'Angelo M, Pallavicini A, Toppo S, Simionati B, Conrad A, Hornischer K, Kauer G, L?hnert TH, Nordsiek G, Reichelt J, Scharfe M, Sch?n O, Bargues M, Terol J, Climent J, Navarro P, Collado C, Perez-Perez A, Ottenw?lder B, Duchemin D, Cooke R, Laudie M, Berger-Llauro C, Purnelle B, Masuy D, de Haan M, Maarse AC, Alcaraz JP, Cottet A, Casacuberta E, Monfort A, Argiriou A, flores M, Liguori R, Vitale D, Mannhaupt G, Haase D, Schoof H, Rudd S, Zaccaria P, Mewes HW, Mayer KF, Kaul S, Town CD, Koo HL, Tallon LJ, Jenkins J, Rooney T, Rizzo M, Walts A, Utterback T, Fujii CY, Shea TP, Creasy TH, Haas B, Maiti R, Wu D, Peterson J, Van Aken S, Pai G, Militscher J, Sellers P, Gill JE, Feldblyum TV, Preuss D, Lin X, Nierman WC, Salzberg SL, White O, Venter JC, Fraser CM, Kaneko T, Nakamura Y, Sato S, Kato T, Asamizu E, Sasamoto S, Kimura T, Idesawa K, Kawashima K, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S, , ,
Nature408(6814)820-2(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that 14-3-3 lambda is required for actin depolymerizing factor 1 (ADF1) phosphorylation and subcellular localization.
Title: 14-3-3 ? protein interacts with ADF1 to regulate actin cytoskeleton dynamics in Arabidopsis.

These results demonstrate a regulatory function of Arabidopsis CDPK6 in the N-terminal phosphorylation of AtADF1.
Title: Arabidopsis CDPK6 phosphorylates ADF1 at N-terminal serine 6 predominantly.

Arabidopsis AtADF1 is functionally affected by mutations on actin binding sites.
Title: Arabidopsis AtADF1 is functionally affected by mutations on actin binding sites.

The following ADF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb12937	NM_114470.3 Latest version!	Arabidopsis thaliana actin depolymerizing factor 1 (ADF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OAb12938	NM_001084778.1 Latest version!	Arabidopsis thaliana actin depolymerizing factor 1 (ADF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ADF1 ( NM_114470.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_190187.1 Arabidopsis thaliana actin depolymerizing factor 1 (ADF1), mRNA.

Price & Availability↑

CloneID	OAb12937
Clone ID Related Accession (Same CDS sequence)	NM_114470.3
Accession Version	NM_114470.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	actin depolymerizing factor 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Sep 12, 2016 this sequence version replaced NM_114470.2.

ATGGCGAACG CGGCATCTGG AATGGCTGTG CATGATGATT GCAAGCTGAG ATTTCTGGAA 
CTGAAGGCTA AAAGGACACA CCGTTTCATA GTTTACAAGA TTGAGGAGAA GCAGAAGCAA 
GTTGTTGTTG AGAAAGTTGG TCAACCGATC CAAACTTACG AGGAGTTTGC AGCATGTCTT 
CCAGCTGATG AATGCCGTTA CGCCATTTAC GATTTTGACT TTGTAACTGC TGAGAATTGC 
CAGAAGAGCA AGATCTTCTT CATCGCGTGG TGTCCGGATA TTGCTAAGGT GAGAAGCAAG 
ATGATCTACG CGAGCTCCAA GGACAGGTTC AAGAGGGAAC TAGATGGGAT TCAAGTAGAG 
CTACAGGCAA CTGATCCAAC AGAGATGGAT CTCGATGTTT TCAGGAGCCG TGCCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_190187.1
CDS	168..587
Translation	MANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAACLPADECRYAIYDFDFVTAENCQKSKIFFIAWCPDIAKVRSKMIYASSKDRFKRELDGIQVELQATDPTEMDLDVFRSRAN

Target ORF information:

RefSeq Version	NM_114470.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana actin depolymerizing factor 1 (ADF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_114470.3

ORF Insert Sequence:

ATGGCGAACG CGGCATCTGG AATGGCTGTG CATGATGATT GCAAGCTGAG ATTTCTGGAA 
CTGAAGGCTA AAAGGACACA CCGTTTCATA GTTTACAAGA TTGAGGAGAA GCAGAAGCAA 
GTTGTTGTTG AGAAAGTTGG TCAACCGATC CAAACTTACG AGGAGTTTGC AGCATGTCTT 
CCAGCTGATG AATGCCGTTA CGCCATTTAC GATTTTGACT TTGTAACTGC TGAGAATTGC 
CAGAAGAGCA AGATCTTCTT CATCGCGTGG TGTCCGGATA TTGCTAAGGT GAGAAGCAAG 
ATGATCTACG CGAGCTCCAA GGACAGGTTC AAGAGGGAAC TAGATGGGAT TCAAGTAGAG 
CTACAGGCAA CTGATCCAAC AGAGATGGAT CTCGATGTTT TCAGGAGCCG TGCCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADF1 ( NM_001084778.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001078247.1 Arabidopsis thaliana actin depolymerizing factor 1 (ADF1), mRNA.

Price & Availability↑

CloneID	OAb12938
Clone ID Related Accession (Same CDS sequence)	NM_001084778.1
Accession Version	NM_001084778.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	actin depolymerizing factor 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074).

ATGGTAAGCT CAATTTTCCA CTTGTTATCT CGATCTGCGA ACGCGGCATC TGGAATGGCT 
GTGCATGATG ATTGCAAGCT GAGATTTCTG GAACTGAAGG CTAAAAGGAC ACACCGTTTC 
ATAGTTTACA AGATTGAGGA GAAGCAGAAG CAAGTTGTTG TTGAGAAAGT TGGTCAACCG 
ATCCAAACTT ACGAGGAGTT TGCAGCATGT CTTCCAGCTG ATGAATGCCG TTACGCCATT 
TACGATTTTG ACTTTGTAAC TGCTGAGAAT TGCCAGAAGA GCAAGATCTT CTTCATCGCG 
TGGTGTCCGG ATATTGCTAA GGTGAGAAGC AAGATGATCT ACGCGAGCTC CAAGGACAGG 
TTCAAGAGGG AACTAGATGG GATTCAAGTA GAGCTACAGG CAACTGATCC AACAGAGATG 
GATCTCGATG TTTTCAGGAG CCGTGCCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001078247.1
CDS	137..589
Translation	MVSSIFHLLSRSANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAACLPADECRYAIYDFDFVTAENCQKSKIFFIAWCPDIAKVRSKMIYASSKDRFKRELDGIQVELQATDPTEMDLDVFRSRAN

Target ORF information:

RefSeq Version	NM_001084778.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana actin depolymerizing factor 1 (ADF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001084778.1

ORF Insert Sequence:

ATGGTAAGCT CAATTTTCCA CTTGTTATCT CGATCTGCGA ACGCGGCATC TGGAATGGCT 
GTGCATGATG ATTGCAAGCT GAGATTTCTG GAACTGAAGG CTAAAAGGAC ACACCGTTTC 
ATAGTTTACA AGATTGAGGA GAAGCAGAAG CAAGTTGTTG TTGAGAAAGT TGGTCAACCG 
ATCCAAACTT ACGAGGAGTT TGCAGCATGT CTTCCAGCTG ATGAATGCCG TTACGCCATT 
TACGATTTTG ACTTTGTAAC TGCTGAGAAT TGCCAGAAGA GCAAGATCTT CTTCATCGCG 
TGGTGTCCGG ATATTGCTAA GGTGAGAAGC AAGATGATCT ACGCGAGCTC CAAGGACAGG 
TTCAAGAGGG AACTAGATGG GATTCAAGTA GAGCTACAGG CAACTGATCC AACAGAGATG 
GATCTCGATG TTTTCAGGAG CCGTGCCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Nature408(6814)820-2(2000 Dec)
Salanoubat M,Lemcke K,Rieger M,Ansorge W,Unseld M,Fartmann B,Valle G,Bl?cker H,Perez-Alonso M,Obermaier B,Delseny M,Boutry M,Grivell LA,Mache R,Puigdom?nech P,De Simone V,Choisne N,Artiguenave F,Robert C,Brottier P,Wincker P,Cattolico L,Weissenbach J,Saurin W,Qu?tier F,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Benes V,Wurmbach E,Drzonek H,Erfle H,Jordan N,Bangert S,Wiedelmann R,Kranz H,Voss H,Holland R,Brandt P,Nyakatura G,Vezzi A,D'Angelo M,Pallavicini A,Toppo S,Simionati B,Conrad A,Hornischer K,Kauer G,L?hnert TH,Nordsiek G,Reichelt J,Scharfe M,Sch?n O,Bargues M,Terol J,Climent J,Navarro P,Collado C,Perez-Perez A,Ottenw?lder B,Duchemin D,Cooke R,Laudie M,Berger-Llauro C,Purnelle B,Masuy D,de Haan M,Maarse AC,Alcaraz JP,Cottet A,Casacuberta E,Monfort A,Argiriou A,flores M,Liguori R,Vitale D,Mannhaupt G,Haase D,Schoof H,Rudd S,Zaccaria P,Mewes HW,Mayer KF,Kaul S,Town CD,Koo HL,Tallon LJ,Jenkins J,Rooney T,Rizzo M,Walts A,Utterback T,Fujii CY,Shea TP,Creasy TH,Haas B,Maiti R,Wu D,Peterson J,Van Aken S,Pai G,Militscher J,Sellers P,Gill JE,Feldblyum TV,Preuss D,Lin X,Nierman WC,Salzberg SL,White O,Venter JC,Fraser CM,Kaneko T,Nakamura Y,Sato S,Kato T,Asamizu E,Sasamoto S,Kimura T,Idesawa K,Kawashima K,Kishida Y,Kiyokawa C,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Tabata S,,,

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ADF1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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