AT3G45810 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AT3G45810
Entrez Gene ID	823724
Full Name	ferric reductase-like transmembrane component family protein
Synonyms	RbohJ,Respiratory burst oxidase homolog J
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_114450.3	NP_190167.2	ferric reductase-like transmembrane component family protein

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os05g0465800	NP_001055783.1
Arabidopsis thaliana (thale cress)	AT3G45810	NP_190167.2
Arabidopsis thaliana (thale cress)	AT5G60010	NP_200809.4
Oryza sativa (rice)	Os01g0835500	NP_001044725.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Salanoubat M, Lemcke K, Rieger M, Ansorge W, Unseld M, Fartmann B, Valle G, Bl?cker H, Perez-Alonso M, Obermaier B, Delseny M, Boutry M, Grivell LA, Mache R, Puigdom?nech P, De Simone V, Choisne N, Artiguenave F, Robert C, Brottier P, Wincker P, Cattolico L, Weissenbach J, Saurin W, Qu?tier F, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Benes V, Wurmbach E, Drzonek H, Erfle H, Jordan N, Bangert S, Wiedelmann R, Kranz H, Voss H, Holland R, Brandt P, Nyakatura G, Vezzi A, D'Angelo M, Pallavicini A, Toppo S, Simionati B, Conrad A, Hornischer K, Kauer G, L?hnert TH, Nordsiek G, Reichelt J, Scharfe M, Sch?n O, Bargues M, Terol J, Climent J, Navarro P, Collado C, Perez-Perez A, Ottenw?lder B, Duchemin D, Cooke R, Laudie M, Berger-Llauro C, Purnelle B, Masuy D, de Haan M, Maarse AC, Alcaraz JP, Cottet A, Casacuberta E, Monfort A, Argiriou A, flores M, Liguori R, Vitale D, Mannhaupt G, Haase D, Schoof H, Rudd S, Zaccaria P, Mewes HW, Mayer KF, Kaul S, Town CD, Koo HL, Tallon LJ, Jenkins J, Rooney T, Rizzo M, Walts A, Utterback T, Fujii CY, Shea TP, Creasy TH, Haas B, Maiti R, Wu D, Peterson J, Van Aken S, Pai G, Militscher J, Sellers P, Gill JE, Feldblyum TV, Preuss D, Lin X, Nierman WC, Salzberg SL, White O, Venter JC, Fraser CM, Kaneko T, Nakamura Y, Sato S, Kato T, Asamizu E, Sasamoto S, Kimura T, Idesawa K, Kawashima K, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S, , ,
Nature408(6814)820-2(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

apoplastic reactive oxygen species derived from RbohH and RbohJ are involved in pollen tube elongation into the stigmatic papillae by affecting the cell wall metabolism.[RbohJ]
Title: Apoplastic ROS production upon pollination by RbohH and RbohJ in Arabidopsis.

RBOHH and RBOHJ were specifically expressed in pollen and dynamically targeted to distinct and overlapping plasma membrane domains at the pollen tube tip. Functional loss of RBOHH and RBOHJ in homozygous double mutants resulted in reduced fertility.
Title: Pollen tube NAD(P)H oxidases act as a speed control to dampen growth rate oscillations during polarized cell growth.

Disruption of the pollen expressed NADPH oxidases RbohH and RbohJ triggers altered reproductive phenotypes.
Title: ANXUR receptor-like kinases coordinate cell wall integrity with growth at the pollen tube tip via NADPH oxidases.

The following AT3G45810 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT3G45810 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb12918	NM_114450.3 Latest version!	Arabidopsis thaliana ferric reductase-like transmembrane component family protein (AT3G45810), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AT3G45810 ( NM_114450.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_190167.2 Arabidopsis thaliana ferric reductase-like transmembrane component family protein (AT3G45810), mRNA.

Price & Availability↑

CloneID	OAb12918
Clone ID Related Accession (Same CDS sequence)	NM_114450.3
Accession Version	NM_114450.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	ferric reductase-like transmembrane component family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Sep 12, 2016 this sequence version replaced NM_114450.2.

ATGAAAAACA ACAAAAAGGT TGGGACCGAA GATTCAACGA AATGGATGCT AGAGAGCGTC 
GAGATAGATC CAAAAGGCGA TAGTTCTGTG AAACAACCCG AAAGCACAAT CAATAGCAAT 
AATCCTGAGA GTAGTGGTGC TGGTGGTGGG ATCTTGAAGA ATGTGTCTAA GAATCTCGCC 
GTGGGATCAA TAATCAGGTC CATGAGCGTA AACAAATGGA GAAAGAGCGG TAATCTCGGG 
TCCCCGAGCA CGAGGAAGAG TGGAAACCTG GGGCCTCCAC TCCCTGTGTC CCAAGTAAAA 
AGACCGGGGC CACAAAGAGT CGAGAGGACT ACATCTAGCG CGGCTAGAGG ACTACAAAGT 
CTCCGGTTCC TTGATCGGAC CGTCACGGGA AGAGAACGAG ATTCGTGGAG ATCAATCGAA 
AACCGGTTCA ATCAATTTGC TGTTGACGGG AGGCTTCCCA AGGATAAATT CGGCGTTTGC 
ATTGGAATGG GAGATACATT GGAGTTTGCG GCAAAAGTAT ACGAAGCATT GGGTAGGAGA 
AGACAAATAA AAACAGAAAA TGGGATTGAC AAGGAACAAC TCAAGCTTTT TTGGGAAGAC 
ATGATCAAGA AGGATCTCGA CTGTCGCCTC CAAATCTTCT TCGACATGTG TGATAAGGAC 
GGAGACGGGA AGTTAACAGA AGAGGAGGTT AAAGAAGTCA TAGTCTTAAG TGCATCCGCG 
AACAGGCTCG TGAATCTGAA GAAGAATGCT GCGTCTTATG CTTCTTTGAT CATGGAAGAG 
CTCGACCCCA ATGAGCAGGG TTACATTGAG ATGTGGCAAC TCGAGGTGCT ACTAACAGGA 
ATTGTATCAA ACGCCGACTC ACATAAGGTC GTGAGGAAAT CGCAACAGCT AACGCGAGCC 
ATGATCCCAA AACGTTACAG AACACCGACT AGTAAATACG TTGTAGTGAC CGCAGAGCTA 
ATGTACGAGC ACTGGAAGAA GATTTGGGTC GTCACATTGT GGCTCGCAGT AAACGTCGTC 
CTCTTCATGT GGAAATACGA AGAGTTCACC ACAAGCCCTC TTTACAACAT CACTGGACGC 
TGCCTTTGCG CCGCTAAAGG AACGGCAGAA ATACTAAAAC TCAACATGGC TTTGATTTTG 
GTCCCTGTTT TAAGAAGAAC CCTAACGTTC CTCAGATCGA CTTTCTTGAA CCACTTGATC 
CCATTCGACG ATAACATCAA CTTCCATAAG TTGATAGCCG TGGCGATAGC AGTTATTTCC 
TTGCTTCACA CGGCATTACA CATGCTATGC AACTACCCTA GGCTAAGTTC TTGCCCCTAC 
AATTTCTACT CCGACTATGC GGGGAATCTC TTGGGAGCCA AGCAACCGAC CTATTTAGGC 
CTAATGCTGA CCCCGGTTTC GGTCACAGGA GTTCTAATGA TCATCTTCAT GGGTATCTCA 
TTCACCCTTG CTATGCATTA CTTCAGAAGA AACATAGTGA AATTGCCTAT ACCATTTAAC 
CGTCTTGCTG GATTTAATTC GTTTTGGTAT GCTCATCATT TGCTGGTTAT TGCCTACGCT 
CTTCTTATCA TCCATGGTTA CATTCTTATC ATCGAGAAAC CTTGGTACCA AAAGACGACG 
TGGATGTACG TGGCCATACC AATGGTGTTG TACGCGAGCG AGAGGCTTTT CTCACGAGTT 
CAAGAACACA ACCATCGTGT CCACATTATC AAAGCTATTG TATATTCAGG CAATGTTCTT 
GCACTCTATA TGACCAAACC TCAAGGGTTC AAGTACAAAA GTGGCATGTA CATGTTTGTC 
AAGTGCCCTG ATATCTCCAA ATTCGAATGG CATCCATTCT CCATCACTTC TGCACCCGGA 
GATGAATATT TGAGCGTTCA CATAAGAGCA TTAGGAGATT GGACTTCTGA ACTTAGGAAC 
AGATTTGCAG AGACATGTGA GCCTCATCAA AAATCGAAAC CTAGTCCAAA TGATCTTATT 
AGGATGGAAA CAAGAGCGAG AGGTGCAAAT CCTCATGTTG AAGAATCCCA AGCCTTATTT 
CCAAGAATAT TTATCAAAGG ACCATATGGG GCTCCGGCAC AAAGCTATCA GAAGTTTGAT 
ATCCTTTTGT TGATTGGGCT AGGGATCGGG GCAACCCCAT TTATAAGTAT CCTAAAAGAT 
ATGTTGAATA ATCTCAAACC TGGTATTCCA AAAACCGGGC AAAAGTATGA AGGAAGTGTA 
GGAGGTGAGA GCTTAGGAGG ATCAAGTGTA TATGGAGGAT CGAGTGTAAA TGGAGGAGGT 
AGTGTAAATG GAGGAGGTAG TGTAAGTGGA GGAGGTAGAA AGTTTCCACA AAGAGCATAT 
TTTTATTGGG TGACAAGAGA ACAAGCTTCT TTCGAATGGT TCAAAGGAGT TATGGATGAT 
ATTGCAGTGT ATGACAAAAC CAATGTGATA GAGATGCATA ACTACTTAAC AAGTATGTAC 
GAAGCAGGAG ATGCGAGATC TGCTCTAATT GCTATGGTCC AGAAACTTCA ACATGCCAAA 
AATGGTGTTG ACATTGTTTC CGAGAGCCGA ATACGAACCC ATTTCGCAAG ACCTAATTGG 
AGGAAGGTTT TTTCTGAATT GTCAAACAAG CACGAGACTT CTCGCATAGG TGTGTTCTAC 
TGTGGAAGTC CAACGCTTGT TCGACCGCTA AAATCACTTT GTCAGGAGTT CAGTCTCGAG 
TCGTCCACCC GCTTTACTTT TCACAAAGAG AATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_190167.2
CDS	224..2962
Translation	MKNNKKVGTEDSTKWMLESVEIDPKGDSSVKQPESTINSNNPESSGAGGGILKNVSKNLAVGSIIRSMSVNKWRKSGNLGSPSTRKSGNLGPPLPVSQVKRPGPQRVERTTSSAARGLQSLRFLDRTVTGRERDSWRSIENRFNQFAVDGRLPKDKFGVCIGMGDTLEFAAKVYEALGRRRQIKTENGIDKEQLKLFWEDMIKKDLDCRLQIFFDMCDKDGDGKLTEEEVKEVIVLSASANRLVNLKKNAASYASLIMEELDPNEQGYIEMWQLEVLLTGIVSNADSHKVVRKSQQLTRAMIPKRYRTPTSKYVVVTAELMYEHWKKIWVVTLWLAVNVVLFMWKYEEFTTSPLYNITGRCLCAAKGTAEILKLNMALILVPVLRRTLTFLRSTFLNHLIPFDDNINFHKLIAVAIAVISLLHTALHMLCNYPRLSSCPYNFYSDYAGNLLGAKQPTYLGLMLTPVSVTGVLMIIFMGISFTLAMHYFRRNIVKLPIPFNRLAGFNSFWYAHHLLVIAYALLIIHGYILIIEKPWYQKTTWMYVAIPMVLYASERLFSRVQEHNHRVHIIKAIVYSGNVLALYMTKPQGFKYKSGMYMFVKCPDISKFEWHPFSITSAPGDEYLSVHIRALGDWTSELRNRFAETCEPHQKSKPSPNDLIRMETRARGANPHVEESQALFPRIFIKGPYGAPAQSYQKFDILLLIGLGIGATPFISILKDMLNNLKPGIPKTGQKYEGSVGGESLGGSSVYGGSSVNGGGSVNGGGSVSGGGRKFPQRAYFYWVTREQASFEWFKGVMDDIAVYDKTNVIEMHNYLTSMYEAGDARSALIAMVQKLQHAKNGVDIVSESRIRTHFARPNWRKVFSELSNKHETSRIGVFYCGSPTLVRPLKSLCQEFSLESSTRFTFHKENF

Target ORF information:

RefSeq Version	NM_114450.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana ferric reductase-like transmembrane component family protein (AT3G45810), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_114450.3

ORF Insert Sequence:

ATGAAAAACA ACAAAAAGGT TGGGACCGAA GATTCAACGA AATGGATGCT AGAGAGCGTC 
GAGATAGATC CAAAAGGCGA TAGTTCTGTG AAACAACCCG AAAGCACAAT CAATAGCAAT 
AATCCTGAGA GTAGTGGTGC TGGTGGTGGG ATCTTGAAGA ATGTGTCTAA GAATCTCGCC 
GTGGGATCAA TAATCAGGTC CATGAGCGTA AACAAATGGA GAAAGAGCGG TAATCTCGGG 
TCCCCGAGCA CGAGGAAGAG TGGAAACCTG GGGCCTCCAC TCCCTGTGTC CCAAGTAAAA 
AGACCGGGGC CACAAAGAGT CGAGAGGACT ACATCTAGCG CGGCTAGAGG ACTACAAAGT 
CTCCGGTTCC TTGATCGGAC CGTCACGGGA AGAGAACGAG ATTCGTGGAG ATCAATCGAA 
AACCGGTTCA ATCAATTTGC TGTTGACGGG AGGCTTCCCA AGGATAAATT CGGCGTTTGC 
ATTGGAATGG GAGATACATT GGAGTTTGCG GCAAAAGTAT ACGAAGCATT GGGTAGGAGA 
AGACAAATAA AAACAGAAAA TGGGATTGAC AAGGAACAAC TCAAGCTTTT TTGGGAAGAC 
ATGATCAAGA AGGATCTCGA CTGTCGCCTC CAAATCTTCT TCGACATGTG TGATAAGGAC 
GGAGACGGGA AGTTAACAGA AGAGGAGGTT AAAGAAGTCA TAGTCTTAAG TGCATCCGCG 
AACAGGCTCG TGAATCTGAA GAAGAATGCT GCGTCTTATG CTTCTTTGAT CATGGAAGAG 
CTCGACCCCA ATGAGCAGGG TTACATTGAG ATGTGGCAAC TCGAGGTGCT ACTAACAGGA 
ATTGTATCAA ACGCCGACTC ACATAAGGTC GTGAGGAAAT CGCAACAGCT AACGCGAGCC 
ATGATCCCAA AACGTTACAG AACACCGACT AGTAAATACG TTGTAGTGAC CGCAGAGCTA 
ATGTACGAGC ACTGGAAGAA GATTTGGGTC GTCACATTGT GGCTCGCAGT AAACGTCGTC 
CTCTTCATGT GGAAATACGA AGAGTTCACC ACAAGCCCTC TTTACAACAT CACTGGACGC 
TGCCTTTGCG CCGCTAAAGG AACGGCAGAA ATACTAAAAC TCAACATGGC TTTGATTTTG 
GTCCCTGTTT TAAGAAGAAC CCTAACGTTC CTCAGATCGA CTTTCTTGAA CCACTTGATC 
CCATTCGACG ATAACATCAA CTTCCATAAG TTGATAGCCG TGGCGATAGC AGTTATTTCC 
TTGCTTCACA CGGCATTACA CATGCTATGC AACTACCCTA GGCTAAGTTC TTGCCCCTAC 
AATTTCTACT CCGACTATGC GGGGAATCTC TTGGGAGCCA AGCAACCGAC CTATTTAGGC 
CTAATGCTGA CCCCGGTTTC GGTCACAGGA GTTCTAATGA TCATCTTCAT GGGTATCTCA 
TTCACCCTTG CTATGCATTA CTTCAGAAGA AACATAGTGA AATTGCCTAT ACCATTTAAC 
CGTCTTGCTG GATTTAATTC GTTTTGGTAT GCTCATCATT TGCTGGTTAT TGCCTACGCT 
CTTCTTATCA TCCATGGTTA CATTCTTATC ATCGAGAAAC CTTGGTACCA AAAGACGACG 
TGGATGTACG TGGCCATACC AATGGTGTTG TACGCGAGCG AGAGGCTTTT CTCACGAGTT 
CAAGAACACA ACCATCGTGT CCACATTATC AAAGCTATTG TATATTCAGG CAATGTTCTT 
GCACTCTATA TGACCAAACC TCAAGGGTTC AAGTACAAAA GTGGCATGTA CATGTTTGTC 
AAGTGCCCTG ATATCTCCAA ATTCGAATGG CATCCATTCT CCATCACTTC TGCACCCGGA 
GATGAATATT TGAGCGTTCA CATAAGAGCA TTAGGAGATT GGACTTCTGA ACTTAGGAAC 
AGATTTGCAG AGACATGTGA GCCTCATCAA AAATCGAAAC CTAGTCCAAA TGATCTTATT 
AGGATGGAAA CAAGAGCGAG AGGTGCAAAT CCTCATGTTG AAGAATCCCA AGCCTTATTT 
CCAAGAATAT TTATCAAAGG ACCATATGGG GCTCCGGCAC AAAGCTATCA GAAGTTTGAT 
ATCCTTTTGT TGATTGGGCT AGGGATCGGG GCAACCCCAT TTATAAGTAT CCTAAAAGAT 
ATGTTGAATA ATCTCAAACC TGGTATTCCA AAAACCGGGC AAAAGTATGA AGGAAGTGTA 
GGAGGTGAGA GCTTAGGAGG ATCAAGTGTA TATGGAGGAT CGAGTGTAAA TGGAGGAGGT 
AGTGTAAATG GAGGAGGTAG TGTAAGTGGA GGAGGTAGAA AGTTTCCACA AAGAGCATAT 
TTTTATTGGG TGACAAGAGA ACAAGCTTCT TTCGAATGGT TCAAAGGAGT TATGGATGAT 
ATTGCAGTGT ATGACAAAAC CAATGTGATA GAGATGCATA ACTACTTAAC AAGTATGTAC 
GAAGCAGGAG ATGCGAGATC TGCTCTAATT GCTATGGTCC AGAAACTTCA ACATGCCAAA 
AATGGTGTTG ACATTGTTTC CGAGAGCCGA ATACGAACCC ATTTCGCAAG ACCTAATTGG 
AGGAAGGTTT TTTCTGAATT GTCAAACAAG CACGAGACTT CTCGCATAGG TGTGTTCTAC 
TGTGGAAGTC CAACGCTTGT TCGACCGCTA AAATCACTTT GTCAGGAGTT CAGTCTCGAG 
TCGTCCACCC GCTTTACTTT TCACAAAGAG AATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Nature408(6814)820-2(2000 Dec)
Salanoubat M,Lemcke K,Rieger M,Ansorge W,Unseld M,Fartmann B,Valle G,Bl?cker H,Perez-Alonso M,Obermaier B,Delseny M,Boutry M,Grivell LA,Mache R,Puigdom?nech P,De Simone V,Choisne N,Artiguenave F,Robert C,Brottier P,Wincker P,Cattolico L,Weissenbach J,Saurin W,Qu?tier F,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Benes V,Wurmbach E,Drzonek H,Erfle H,Jordan N,Bangert S,Wiedelmann R,Kranz H,Voss H,Holland R,Brandt P,Nyakatura G,Vezzi A,D'Angelo M,Pallavicini A,Toppo S,Simionati B,Conrad A,Hornischer K,Kauer G,L?hnert TH,Nordsiek G,Reichelt J,Scharfe M,Sch?n O,Bargues M,Terol J,Climent J,Navarro P,Collado C,Perez-Perez A,Ottenw?lder B,Duchemin D,Cooke R,Laudie M,Berger-Llauro C,Purnelle B,Masuy D,de Haan M,Maarse AC,Alcaraz JP,Cottet A,Casacuberta E,Monfort A,Argiriou A,flores M,Liguori R,Vitale D,Mannhaupt G,Haase D,Schoof H,Rudd S,Zaccaria P,Mewes HW,Mayer KF,Kaul S,Town CD,Koo HL,Tallon LJ,Jenkins J,Rooney T,Rizzo M,Walts A,Utterback T,Fujii CY,Shea TP,Creasy TH,Haas B,Maiti R,Wu D,Peterson J,Van Aken S,Pai G,Militscher J,Sellers P,Gill JE,Feldblyum TV,Preuss D,Lin X,Nierman WC,Salzberg SL,White O,Venter JC,Fraser CM,Kaneko T,Nakamura Y,Sato S,Kato T,Asamizu E,Sasamoto S,Kimura T,Idesawa K,Kawashima K,Kishida Y,Kiyokawa C,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Tabata S,,,

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AT3G45810 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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