HGL1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	HGL1
Entrez Gene ID	821944
Full Name	heteroglycan glucosidase 1
Synonyms	heteroglycan glucosidase 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_113268.3	NP_566736.1	heteroglycan glucosidase 1
NM_001125213.2	NP_001118685.1	heteroglycan glucosidase 1
NM_001338653.1	NP_001327915.1	heteroglycan glucosidase 1

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os07g0421300	NP_001059483.1
Arabidopsis thaliana (thale cress)	HGL1	NP_566736.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Salanoubat M, Lemcke K, Rieger M, Ansorge W, Unseld M, Fartmann B, Valle G, Bl?cker H, Perez-Alonso M, Obermaier B, Delseny M, Boutry M, Grivell LA, Mache R, Puigdom?nech P, De Simone V, Choisne N, Artiguenave F, Robert C, Brottier P, Wincker P, Cattolico L, Weissenbach J, Saurin W, Qu?tier F, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Benes V, Wurmbach E, Drzonek H, Erfle H, Jordan N, Bangert S, Wiedelmann R, Kranz H, Voss H, Holland R, Brandt P, Nyakatura G, Vezzi A, D'Angelo M, Pallavicini A, Toppo S, Simionati B, Conrad A, Hornischer K, Kauer G, L?hnert TH, Nordsiek G, Reichelt J, Scharfe M, Sch?n O, Bargues M, Terol J, Climent J, Navarro P, Collado C, Perez-Perez A, Ottenw?lder B, Duchemin D, Cooke R, Laudie M, Berger-Llauro C, Purnelle B, Masuy D, de Haan M, Maarse AC, Alcaraz JP, Cottet A, Casacuberta E, Monfort A, Argiriou A, flores M, Liguori R, Vitale D, Mannhaupt G, Haase D, Schoof H, Rudd S, Zaccaria P, Mewes HW, Mayer KF, Kaul S, Town CD, Koo HL, Tallon LJ, Jenkins J, Rooney T, Rizzo M, Walts A, Utterback T, Fujii CY, Shea TP, Creasy TH, Haas B, Maiti R, Wu D, Peterson J, Van Aken S, Pai G, Militscher J, Sellers P, Gill JE, Feldblyum TV, Preuss D, Lin X, Nierman WC, Salzberg SL, White O, Venter JC, Fraser CM, Kaneko T, Nakamura Y, Sato S, Kato T, Asamizu E, Sasamoto S, Kimura T, Idesawa K, Kawashima K, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S, , ,
Nature408(6814)820-2(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HGL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HGL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb12247	NM_113268.3 Latest version!	Arabidopsis thaliana heteroglycan glucosidase 1 (HGL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb12247	NM_001125213.2 Latest version!	Arabidopsis thaliana heteroglycan glucosidase 1 (HGL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb37726	NM_001338653.1 Latest version!	Arabidopsis thaliana heteroglycan glucosidase 1 (HGL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HGL1 ( NM_113268.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_566736.1 Arabidopsis thaliana heteroglycan glucosidase 1 (HGL1), mRNA.

Price & Availability↑

CloneID	OAb12247
Clone ID Related Accession (Same CDS sequence)	NM_113268.3 , NM_001125213.2
Accession Version	NM_113268.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	heteroglycan glucosidase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Apr 24, 2008 this sequence version replaced NM_113268.2.

ATGACTTTAA GTGGGGATAG CTCGGAAACT GTAGAGATGA CTTCAACTGA TATGATTTTC 
GAGCCGATTT TGGAGCATGG AGTTTTCCGG TTTGATTGTT CTGTTGACCA TAGGAAGGCT 
GCTTTTCCTA GTGTTTCATT TAAGAACAGC AAAGACCGGG AAGTTCCTAT TGTGAGTCAC 
ATTGTTCCAG CATATATTCC TACTTGTGGT TGTCTTCAGG ACCAACAAGT TGTCACCTTT 
GAGTTTTCTC CTGGTACATC GTTTTATGGG ACGGGAGAAG TTAGTGGTCA GTTGGAGAGA 
ACAGGCAAAC GGGTTTTTAC TTGGAACACT GATGCATGGG GATATGGTTC TGGAACCACT 
TCGTTATACC AGTCACATCC TTGGGTACTA GTTGTCCTTC CAACTGGAGA AACACTAGGG 
GTTCTTGCTG ATACAACACG AAAGTGTGAG ATTGATCTAC GGAAAGAGGG AATCATAAGA 
ATTATCTCCC CAGCATCATA TCCAATAATC ACATTTGGTC CATTCTCCTC GCCTACTGCT 
GTCTTGGAGT CCCTCTCGCA CGCCATTGGA ACTGTGTTTA TGCCTCCGAA GTGGGCCTTA 
GGCTACCACC AATGCCGTTG GAGTTATATG TCTGACAAGC GAGTAGCCGA GATCGCTCAA 
ACATTTCGCG ACAAGAAAAT TCCTTCTGAC GTGATATGGA TGGATATTGA CTACATGGAT 
GGTTTCCGTT GTTTCACATT CGACAAGGAG CGTTTTCCGG ATCCAAGTGC TTTGGCCAAA 
GATCTCCATA GTAATGGTTT CAAAGCTATC TGGATGCTTG ACCCTGGAAT AAAGCAGGAG 
GAAGGTTATT ATGTCTACGA TAGTGGGAGT AAAAATGATG TTTGGATTAG CCGAGCAGAT 
GGGAAGCCAT TTACTGGTGA AGTATGGCCT GGGCCTTGCG TTTTCCCTGA CTATACAAAT 
TCCAAAGCAC GCTCTTGGTG GGCTAATTTG GTTAAGGAAT TTGTTTCAAA TGGTGTTGAT 
GGTATATGGA ATGATATGAA TGAGCCAGCT GTTTTCAAAG TCGTGACAAA GACGATGCCT 
GAGAACAATA TTCACCATGG AGATGATGAG CTTGGAGGTG TTCAAAACCA CTCACACTAC 
CACAATGTCT ATGGGATGCT GATGGCAAGA TCAACTTACG AAGGGATGGA GCTAGCTGAT 
AAGAATAAGC GACCTTTTGT ATTGACAAGG GCTGGATTCA TAGGTAGCCA GAGATATGCT 
GCGACTTGGA CAGGAGATAA CCTTTCAAAC TGGGAGCATC TACATATGTC TATATCCATG 
GTCCTTCAGC TGGGGCTTAG TGGTCAGCCC TTATCAGGGC CAGATATCGG TGGCTTTGCT 
GGCAATGCAA CTCCGCGGCT ATTTGGACGT TGGATGGGTG TTGGAGCTAT GTTTCCATTC 
TGTCGTGGCC ATTCCGAGGC TGGCACTGAT GACCATGAAC CATGGTCCTT TGGAGAAGAG 
TGTGAGGAAG TCTGTCGTGC CGCATTGAAG AGGCGTTACC AACTCCTACC ACATTTTTAC 
ACCTTGTTTT ACATAGCCCA TACAACTGGT GCTCCAGTTG CAGCTCCAAT CTTTTTCGCT 
GATCCAATAG ATTCCCGGCT AAGAGCTGTT GAAAATGGCT TTCTTTTGGG CCCACTTCTT 
ATATATGCAA GCACTTTGTC TAGTCAGGGA TCACATGAGC TGCAGCACAT ATTACCGAGA 
GGAATTTGGC ATAGGTTTGA TTTTGCAGAT TCACATCCGG ATTTACCAAC TTTATATTTA 
CAAGGGGGAT CCATCATATC ATTGGCTCCT CCACATCTGC ATGTTGGGGA GTTTAGTCTG 
TCAGATGACT TGACCCTACT TGTGTCATTA GATGAAAATG GGAAAGCTAA AGGACTCTTG 
TTCGAAGATG ATGGAGATGG ATATGGCTAC ACTAAAGGAA GATTTCTAGT TACACACTAC 
ATTGCTGAGC GGGATTCTTC CACTGTCACC GTCAAGGTTT CAAAAACTGA AGGAGACTGG 
CAGAGGCCAA ACCGCCGTGT TCATGTCCAA TTATTACTAG GAGGCGGTGC AATGCTTGAT 
GCTTGGGGAA TGGATGGAGA GTTTATCCAC ATTAAGGTGC CTTCAGAAAG TGGAATTTCA 
GAGTTAATAT CCACAAGCAA TGAGCGCTTT AAACTTCATA TGGAAAATAC AAAACTGATA 
CCTGAGAAGG AAGTGGTACC TGGACAAAAG GGAATGGAAC TTTCAAAGGA GCCAGTTGAG 
TTAAGCAGTG GTGATTGGAA ACTTAATATA GTTCCCTGGG TTGGTGGAAG GATATTGTCT 
ATGACACATG TTCCCTCAGG AATTCAATGG CTTCATAGCA GGATAGATAT CAATGGTTAT 
GAGGAGTATA GTGGTACTGA GTACCGGTCA GCTGGATGTA CTGAGGAATA CAATGTCATT 
GAGAGGGATT TGGAGCATGC AGGAGAAGAA GAATCTCTGA TCCTAGAAGG TGATGTAGGT 
GGTGGACTGG TTCTTCGGCG TAAAATAAGT ATAGCTAAAG ACAATCAACG AGTTTTCCGG 
ATTGCCTCTA GCATTGAAGC CCGTAGTGTT GGTGCTGGTT CTGGTGGATT TTCAAGGCTG 
GTATGCTTAA GAGTCCATCC AACTTTCACT CTTTTGCATC CGACTGAATC GTTTGTCTCT 
TTCACGTCGA TTGATGGTTC AAAGCATGAA GTTTGGCCAG ATTCTGGAGA CCAAATATAT 
GAAGGGAACA ACCTTCCACA TGGGAAATGG ATGTTGGTGG ACAAGAGCCT CAATCTTCGG 
ATGGTAAACA GGTTCGATGT TAGTCAGGTC TTCAAATGTA TTATCCACTG GGACTGTGGA 
ACTGTTAACT TAGAGCTATG GTCTAAAGAG CGGCCTGTTT CCAAGGAATC TCCTCTGAAA 
ATAGAGCATG AATATGAAGT CACAAGTTTC CCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_566736.1
CDS	206..3181
Translation	MTLSGDSSETVEMTSTDMIFEPILEHGVFRFDCSVDHRKAAFPSVSFKNSKDREVPIVSHIVPAYIPTCGCLQDQQVVTFEFSPGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGSGTTSLYQSHPWVLVVLPTGETLGVLADTTRKCEIDLRKEGIIRIISPASYPIITFGPFSSPTAVLESLSHAIGTVFMPPKWALGYHQCRWSYMSDKRVAEIAQTFRDKKIPSDVIWMDIDYMDGFRCFTFDKERFPDPSALAKDLHSNGFKAIWMLDPGIKQEEGYYVYDSGSKNDVWISRADGKPFTGEVWPGPCVFPDYTNSKARSWWANLVKEFVSNGVDGIWNDMNEPAVFKVVTKTMPENNIHHGDDELGGVQNHSHYHNVYGMLMARSTYEGMELADKNKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPRLFGRWMGVGAMFPFCRGHSEAGTDDHEPWSFGEECEEVCRAALKRRYQLLPHFYTLFYIAHTTGAPVAAPIFFADPIDSRLRAVENGFLLGPLLIYASTLSSQGSHELQHILPRGIWHRFDFADSHPDLPTLYLQGGSIISLAPPHLHVGEFSLSDDLTLLVSLDENGKAKGLLFEDDGDGYGYTKGRFLVTHYIAERDSSTVTVKVSKTEGDWQRPNRRVHVQLLLGGGAMLDAWGMDGEFIHIKVPSESGISELISTSNERFKLHMENTKLIPEKEVVPGQKGMELSKEPVELSSGDWKLNIVPWVGGRILSMTHVPSGIQWLHSRIDINGYEEYSGTEYRSAGCTEEYNVIERDLEHAGEEESLILEGDVGGGLVLRRKISIAKDNQRVFRIASSIEARSVGAGSGGFSRLVCLRVHPTFTLLHPTESFVSFTSIDGSKHEVWPDSGDQIYEGNNLPHGKWMLVDKSLNLRMVNRFDVSQVFKCIIHWDCGTVNLELWSKERPVSKESPLKIEHEYEVTSFP

Target ORF information:

RefSeq Version	NM_113268.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana heteroglycan glucosidase 1 (HGL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_113268.3

ORF Insert Sequence:

ATGACTTTAA GTGGGGATAG CTCGGAAACT GTAGAGATGA CTTCAACTGA TATGATTTTC 
GAGCCGATTT TGGAGCATGG AGTTTTCCGG TTTGATTGTT CTGTTGACCA TAGGAAGGCT 
GCTTTTCCTA GTGTTTCATT TAAGAACAGC AAAGACCGGG AAGTTCCTAT TGTGAGTCAC 
ATTGTTCCAG CATATATTCC TACTTGTGGT TGTCTTCAGG ACCAACAAGT TGTCACCTTT 
GAGTTTTCTC CTGGTACATC GTTTTATGGG ACGGGAGAAG TTAGTGGTCA GTTGGAGAGA 
ACAGGCAAAC GGGTTTTTAC TTGGAACACT GATGCATGGG GATATGGTTC TGGAACCACT 
TCGTTATACC AGTCACATCC TTGGGTACTA GTTGTCCTTC CAACTGGAGA AACACTAGGG 
GTTCTTGCTG ATACAACACG AAAGTGTGAG ATTGATCTAC GGAAAGAGGG AATCATAAGA 
ATTATCTCCC CAGCATCATA TCCAATAATC ACATTTGGTC CATTCTCCTC GCCTACTGCT 
GTCTTGGAGT CCCTCTCGCA CGCCATTGGA ACTGTGTTTA TGCCTCCGAA GTGGGCCTTA 
GGCTACCACC AATGCCGTTG GAGTTATATG TCTGACAAGC GAGTAGCCGA GATCGCTCAA 
ACATTTCGCG ACAAGAAAAT TCCTTCTGAC GTGATATGGA TGGATATTGA CTACATGGAT 
GGTTTCCGTT GTTTCACATT CGACAAGGAG CGTTTTCCGG ATCCAAGTGC TTTGGCCAAA 
GATCTCCATA GTAATGGTTT CAAAGCTATC TGGATGCTTG ACCCTGGAAT AAAGCAGGAG 
GAAGGTTATT ATGTCTACGA TAGTGGGAGT AAAAATGATG TTTGGATTAG CCGAGCAGAT 
GGGAAGCCAT TTACTGGTGA AGTATGGCCT GGGCCTTGCG TTTTCCCTGA CTATACAAAT 
TCCAAAGCAC GCTCTTGGTG GGCTAATTTG GTTAAGGAAT TTGTTTCAAA TGGTGTTGAT 
GGTATATGGA ATGATATGAA TGAGCCAGCT GTTTTCAAAG TCGTGACAAA GACGATGCCT 
GAGAACAATA TTCACCATGG AGATGATGAG CTTGGAGGTG TTCAAAACCA CTCACACTAC 
CACAATGTCT ATGGGATGCT GATGGCAAGA TCAACTTACG AAGGGATGGA GCTAGCTGAT 
AAGAATAAGC GACCTTTTGT ATTGACAAGG GCTGGATTCA TAGGTAGCCA GAGATATGCT 
GCGACTTGGA CAGGAGATAA CCTTTCAAAC TGGGAGCATC TACATATGTC TATATCCATG 
GTCCTTCAGC TGGGGCTTAG TGGTCAGCCC TTATCAGGGC CAGATATCGG TGGCTTTGCT 
GGCAATGCAA CTCCGCGGCT ATTTGGACGT TGGATGGGTG TTGGAGCTAT GTTTCCATTC 
TGTCGTGGCC ATTCCGAGGC TGGCACTGAT GACCATGAAC CATGGTCCTT TGGAGAAGAG 
TGTGAGGAAG TCTGTCGTGC CGCATTGAAG AGGCGTTACC AACTCCTACC ACATTTTTAC 
ACCTTGTTTT ACATAGCCCA TACAACTGGT GCTCCAGTTG CAGCTCCAAT CTTTTTCGCT 
GATCCAATAG ATTCCCGGCT AAGAGCTGTT GAAAATGGCT TTCTTTTGGG CCCACTTCTT 
ATATATGCAA GCACTTTGTC TAGTCAGGGA TCACATGAGC TGCAGCACAT ATTACCGAGA 
GGAATTTGGC ATAGGTTTGA TTTTGCAGAT TCACATCCGG ATTTACCAAC TTTATATTTA 
CAAGGGGGAT CCATCATATC ATTGGCTCCT CCACATCTGC ATGTTGGGGA GTTTAGTCTG 
TCAGATGACT TGACCCTACT TGTGTCATTA GATGAAAATG GGAAAGCTAA AGGACTCTTG 
TTCGAAGATG ATGGAGATGG ATATGGCTAC ACTAAAGGAA GATTTCTAGT TACACACTAC 
ATTGCTGAGC GGGATTCTTC CACTGTCACC GTCAAGGTTT CAAAAACTGA AGGAGACTGG 
CAGAGGCCAA ACCGCCGTGT TCATGTCCAA TTATTACTAG GAGGCGGTGC AATGCTTGAT 
GCTTGGGGAA TGGATGGAGA GTTTATCCAC ATTAAGGTGC CTTCAGAAAG TGGAATTTCA 
GAGTTAATAT CCACAAGCAA TGAGCGCTTT AAACTTCATA TGGAAAATAC AAAACTGATA 
CCTGAGAAGG AAGTGGTACC TGGACAAAAG GGAATGGAAC TTTCAAAGGA GCCAGTTGAG 
TTAAGCAGTG GTGATTGGAA ACTTAATATA GTTCCCTGGG TTGGTGGAAG GATATTGTCT 
ATGACACATG TTCCCTCAGG AATTCAATGG CTTCATAGCA GGATAGATAT CAATGGTTAT 
GAGGAGTATA GTGGTACTGA GTACCGGTCA GCTGGATGTA CTGAGGAATA CAATGTCATT 
GAGAGGGATT TGGAGCATGC AGGAGAAGAA GAATCTCTGA TCCTAGAAGG TGATGTAGGT 
GGTGGACTGG TTCTTCGGCG TAAAATAAGT ATAGCTAAAG ACAATCAACG AGTTTTCCGG 
ATTGCCTCTA GCATTGAAGC CCGTAGTGTT GGTGCTGGTT CTGGTGGATT TTCAAGGCTG 
GTATGCTTAA GAGTCCATCC AACTTTCACT CTTTTGCATC CGACTGAATC GTTTGTCTCT 
TTCACGTCGA TTGATGGTTC AAAGCATGAA GTTTGGCCAG ATTCTGGAGA CCAAATATAT 
GAAGGGAACA ACCTTCCACA TGGGAAATGG ATGTTGGTGG ACAAGAGCCT CAATCTTCGG 
ATGGTAAACA GGTTCGATGT TAGTCAGGTC TTCAAATGTA TTATCCACTG GGACTGTGGA 
ACTGTTAACT TAGAGCTATG GTCTAAAGAG CGGCCTGTTT CCAAGGAATC TCCTCTGAAA 
ATAGAGCATG AATATGAAGT CACAAGTTTC CCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HGL1 ( NM_001125213.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001118685.1 Arabidopsis thaliana heteroglycan glucosidase 1 (HGL1), mRNA.

Price & Availability↑

CloneID	OAb12247
Clone ID Related Accession (Same CDS sequence)	NM_113268.3 , NM_001125213.2
Accession Version	NM_001125213.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	heteroglycan glucosidase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Sep 12, 2016 this sequence version replaced NM_001125213.1.

ATGACTTTAA GTGGGGATAG CTCGGAAACT GTAGAGATGA CTTCAACTGA TATGATTTTC 
GAGCCGATTT TGGAGCATGG AGTTTTCCGG TTTGATTGTT CTGTTGACCA TAGGAAGGCT 
GCTTTTCCTA GTGTTTCATT TAAGAACAGC AAAGACCGGG AAGTTCCTAT TGTGAGTCAC 
ATTGTTCCAG CATATATTCC TACTTGTGGT TGTCTTCAGG ACCAACAAGT TGTCACCTTT 
GAGTTTTCTC CTGGTACATC GTTTTATGGG ACGGGAGAAG TTAGTGGTCA GTTGGAGAGA 
ACAGGCAAAC GGGTTTTTAC TTGGAACACT GATGCATGGG GATATGGTTC TGGAACCACT 
TCGTTATACC AGTCACATCC TTGGGTACTA GTTGTCCTTC CAACTGGAGA AACACTAGGG 
GTTCTTGCTG ATACAACACG AAAGTGTGAG ATTGATCTAC GGAAAGAGGG AATCATAAGA 
ATTATCTCCC CAGCATCATA TCCAATAATC ACATTTGGTC CATTCTCCTC GCCTACTGCT 
GTCTTGGAGT CCCTCTCGCA CGCCATTGGA ACTGTGTTTA TGCCTCCGAA GTGGGCCTTA 
GGCTACCACC AATGCCGTTG GAGTTATATG TCTGACAAGC GAGTAGCCGA GATCGCTCAA 
ACATTTCGCG ACAAGAAAAT TCCTTCTGAC GTGATATGGA TGGATATTGA CTACATGGAT 
GGTTTCCGTT GTTTCACATT CGACAAGGAG CGTTTTCCGG ATCCAAGTGC TTTGGCCAAA 
GATCTCCATA GTAATGGTTT CAAAGCTATC TGGATGCTTG ACCCTGGAAT AAAGCAGGAG 
GAAGGTTATT ATGTCTACGA TAGTGGGAGT AAAAATGATG TTTGGATTAG CCGAGCAGAT 
GGGAAGCCAT TTACTGGTGA AGTATGGCCT GGGCCTTGCG TTTTCCCTGA CTATACAAAT 
TCCAAAGCAC GCTCTTGGTG GGCTAATTTG GTTAAGGAAT TTGTTTCAAA TGGTGTTGAT 
GGTATATGGA ATGATATGAA TGAGCCAGCT GTTTTCAAAG TCGTGACAAA GACGATGCCT 
GAGAACAATA TTCACCATGG AGATGATGAG CTTGGAGGTG TTCAAAACCA CTCACACTAC 
CACAATGTCT ATGGGATGCT GATGGCAAGA TCAACTTACG AAGGGATGGA GCTAGCTGAT 
AAGAATAAGC GACCTTTTGT ATTGACAAGG GCTGGATTCA TAGGTAGCCA GAGATATGCT 
GCGACTTGGA CAGGAGATAA CCTTTCAAAC TGGGAGCATC TACATATGTC TATATCCATG 
GTCCTTCAGC TGGGGCTTAG TGGTCAGCCC TTATCAGGGC CAGATATCGG TGGCTTTGCT 
GGCAATGCAA CTCCGCGGCT ATTTGGACGT TGGATGGGTG TTGGAGCTAT GTTTCCATTC 
TGTCGTGGCC ATTCCGAGGC TGGCACTGAT GACCATGAAC CATGGTCCTT TGGAGAAGAG 
TGTGAGGAAG TCTGTCGTGC CGCATTGAAG AGGCGTTACC AACTCCTACC ACATTTTTAC 
ACCTTGTTTT ACATAGCCCA TACAACTGGT GCTCCAGTTG CAGCTCCAAT CTTTTTCGCT 
GATCCAATAG ATTCCCGGCT AAGAGCTGTT GAAAATGGCT TTCTTTTGGG CCCACTTCTT 
ATATATGCAA GCACTTTGTC TAGTCAGGGA TCACATGAGC TGCAGCACAT ATTACCGAGA 
GGAATTTGGC ATAGGTTTGA TTTTGCAGAT TCACATCCGG ATTTACCAAC TTTATATTTA 
CAAGGGGGAT CCATCATATC ATTGGCTCCT CCACATCTGC ATGTTGGGGA GTTTAGTCTG 
TCAGATGACT TGACCCTACT TGTGTCATTA GATGAAAATG GGAAAGCTAA AGGACTCTTG 
TTCGAAGATG ATGGAGATGG ATATGGCTAC ACTAAAGGAA GATTTCTAGT TACACACTAC 
ATTGCTGAGC GGGATTCTTC CACTGTCACC GTCAAGGTTT CAAAAACTGA AGGAGACTGG 
CAGAGGCCAA ACCGCCGTGT TCATGTCCAA TTATTACTAG GAGGCGGTGC AATGCTTGAT 
GCTTGGGGAA TGGATGGAGA GTTTATCCAC ATTAAGGTGC CTTCAGAAAG TGGAATTTCA 
GAGTTAATAT CCACAAGCAA TGAGCGCTTT AAACTTCATA TGGAAAATAC AAAACTGATA 
CCTGAGAAGG AAGTGGTACC TGGACAAAAG GGAATGGAAC TTTCAAAGGA GCCAGTTGAG 
TTAAGCAGTG GTGATTGGAA ACTTAATATA GTTCCCTGGG TTGGTGGAAG GATATTGTCT 
ATGACACATG TTCCCTCAGG AATTCAATGG CTTCATAGCA GGATAGATAT CAATGGTTAT 
GAGGAGTATA GTGGTACTGA GTACCGGTCA GCTGGATGTA CTGAGGAATA CAATGTCATT 
GAGAGGGATT TGGAGCATGC AGGAGAAGAA GAATCTCTGA TCCTAGAAGG TGATGTAGGT 
GGTGGACTGG TTCTTCGGCG TAAAATAAGT ATAGCTAAAG ACAATCAACG AGTTTTCCGG 
ATTGCCTCTA GCATTGAAGC CCGTAGTGTT GGTGCTGGTT CTGGTGGATT TTCAAGGCTG 
GTATGCTTAA GAGTCCATCC AACTTTCACT CTTTTGCATC CGACTGAATC GTTTGTCTCT 
TTCACGTCGA TTGATGGTTC AAAGCATGAA GTTTGGCCAG ATTCTGGAGA CCAAATATAT 
GAAGGGAACA ACCTTCCACA TGGGAAATGG ATGTTGGTGG ACAAGAGCCT CAATCTTCGG 
ATGGTAAACA GGTTCGATGT TAGTCAGGTC TTCAAATGTA TTATCCACTG GGACTGTGGA 
ACTGTTAACT TAGAGCTATG GTCTAAAGAG CGGCCTGTTT CCAAGGAATC TCCTCTGAAA 
ATAGAGCATG AATATGAAGT CACAAGTTTC CCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001118685.1
CDS	483..3458
Translation	MTLSGDSSETVEMTSTDMIFEPILEHGVFRFDCSVDHRKAAFPSVSFKNSKDREVPIVSHIVPAYIPTCGCLQDQQVVTFEFSPGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGSGTTSLYQSHPWVLVVLPTGETLGVLADTTRKCEIDLRKEGIIRIISPASYPIITFGPFSSPTAVLESLSHAIGTVFMPPKWALGYHQCRWSYMSDKRVAEIAQTFRDKKIPSDVIWMDIDYMDGFRCFTFDKERFPDPSALAKDLHSNGFKAIWMLDPGIKQEEGYYVYDSGSKNDVWISRADGKPFTGEVWPGPCVFPDYTNSKARSWWANLVKEFVSNGVDGIWNDMNEPAVFKVVTKTMPENNIHHGDDELGGVQNHSHYHNVYGMLMARSTYEGMELADKNKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPRLFGRWMGVGAMFPFCRGHSEAGTDDHEPWSFGEECEEVCRAALKRRYQLLPHFYTLFYIAHTTGAPVAAPIFFADPIDSRLRAVENGFLLGPLLIYASTLSSQGSHELQHILPRGIWHRFDFADSHPDLPTLYLQGGSIISLAPPHLHVGEFSLSDDLTLLVSLDENGKAKGLLFEDDGDGYGYTKGRFLVTHYIAERDSSTVTVKVSKTEGDWQRPNRRVHVQLLLGGGAMLDAWGMDGEFIHIKVPSESGISELISTSNERFKLHMENTKLIPEKEVVPGQKGMELSKEPVELSSGDWKLNIVPWVGGRILSMTHVPSGIQWLHSRIDINGYEEYSGTEYRSAGCTEEYNVIERDLEHAGEEESLILEGDVGGGLVLRRKISIAKDNQRVFRIASSIEARSVGAGSGGFSRLVCLRVHPTFTLLHPTESFVSFTSIDGSKHEVWPDSGDQIYEGNNLPHGKWMLVDKSLNLRMVNRFDVSQVFKCIIHWDCGTVNLELWSKERPVSKESPLKIEHEYEVTSFP

Target ORF information:

RefSeq Version	NM_001125213.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana heteroglycan glucosidase 1 (HGL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001125213.2

ORF Insert Sequence:

ATGACTTTAA GTGGGGATAG CTCGGAAACT GTAGAGATGA CTTCAACTGA TATGATTTTC 
GAGCCGATTT TGGAGCATGG AGTTTTCCGG TTTGATTGTT CTGTTGACCA TAGGAAGGCT 
GCTTTTCCTA GTGTTTCATT TAAGAACAGC AAAGACCGGG AAGTTCCTAT TGTGAGTCAC 
ATTGTTCCAG CATATATTCC TACTTGTGGT TGTCTTCAGG ACCAACAAGT TGTCACCTTT 
GAGTTTTCTC CTGGTACATC GTTTTATGGG ACGGGAGAAG TTAGTGGTCA GTTGGAGAGA 
ACAGGCAAAC GGGTTTTTAC TTGGAACACT GATGCATGGG GATATGGTTC TGGAACCACT 
TCGTTATACC AGTCACATCC TTGGGTACTA GTTGTCCTTC CAACTGGAGA AACACTAGGG 
GTTCTTGCTG ATACAACACG AAAGTGTGAG ATTGATCTAC GGAAAGAGGG AATCATAAGA 
ATTATCTCCC CAGCATCATA TCCAATAATC ACATTTGGTC CATTCTCCTC GCCTACTGCT 
GTCTTGGAGT CCCTCTCGCA CGCCATTGGA ACTGTGTTTA TGCCTCCGAA GTGGGCCTTA 
GGCTACCACC AATGCCGTTG GAGTTATATG TCTGACAAGC GAGTAGCCGA GATCGCTCAA 
ACATTTCGCG ACAAGAAAAT TCCTTCTGAC GTGATATGGA TGGATATTGA CTACATGGAT 
GGTTTCCGTT GTTTCACATT CGACAAGGAG CGTTTTCCGG ATCCAAGTGC TTTGGCCAAA 
GATCTCCATA GTAATGGTTT CAAAGCTATC TGGATGCTTG ACCCTGGAAT AAAGCAGGAG 
GAAGGTTATT ATGTCTACGA TAGTGGGAGT AAAAATGATG TTTGGATTAG CCGAGCAGAT 
GGGAAGCCAT TTACTGGTGA AGTATGGCCT GGGCCTTGCG TTTTCCCTGA CTATACAAAT 
TCCAAAGCAC GCTCTTGGTG GGCTAATTTG GTTAAGGAAT TTGTTTCAAA TGGTGTTGAT 
GGTATATGGA ATGATATGAA TGAGCCAGCT GTTTTCAAAG TCGTGACAAA GACGATGCCT 
GAGAACAATA TTCACCATGG AGATGATGAG CTTGGAGGTG TTCAAAACCA CTCACACTAC 
CACAATGTCT ATGGGATGCT GATGGCAAGA TCAACTTACG AAGGGATGGA GCTAGCTGAT 
AAGAATAAGC GACCTTTTGT ATTGACAAGG GCTGGATTCA TAGGTAGCCA GAGATATGCT 
GCGACTTGGA CAGGAGATAA CCTTTCAAAC TGGGAGCATC TACATATGTC TATATCCATG 
GTCCTTCAGC TGGGGCTTAG TGGTCAGCCC TTATCAGGGC CAGATATCGG TGGCTTTGCT 
GGCAATGCAA CTCCGCGGCT ATTTGGACGT TGGATGGGTG TTGGAGCTAT GTTTCCATTC 
TGTCGTGGCC ATTCCGAGGC TGGCACTGAT GACCATGAAC CATGGTCCTT TGGAGAAGAG 
TGTGAGGAAG TCTGTCGTGC CGCATTGAAG AGGCGTTACC AACTCCTACC ACATTTTTAC 
ACCTTGTTTT ACATAGCCCA TACAACTGGT GCTCCAGTTG CAGCTCCAAT CTTTTTCGCT 
GATCCAATAG ATTCCCGGCT AAGAGCTGTT GAAAATGGCT TTCTTTTGGG CCCACTTCTT 
ATATATGCAA GCACTTTGTC TAGTCAGGGA TCACATGAGC TGCAGCACAT ATTACCGAGA 
GGAATTTGGC ATAGGTTTGA TTTTGCAGAT TCACATCCGG ATTTACCAAC TTTATATTTA 
CAAGGGGGAT CCATCATATC ATTGGCTCCT CCACATCTGC ATGTTGGGGA GTTTAGTCTG 
TCAGATGACT TGACCCTACT TGTGTCATTA GATGAAAATG GGAAAGCTAA AGGACTCTTG 
TTCGAAGATG ATGGAGATGG ATATGGCTAC ACTAAAGGAA GATTTCTAGT TACACACTAC 
ATTGCTGAGC GGGATTCTTC CACTGTCACC GTCAAGGTTT CAAAAACTGA AGGAGACTGG 
CAGAGGCCAA ACCGCCGTGT TCATGTCCAA TTATTACTAG GAGGCGGTGC AATGCTTGAT 
GCTTGGGGAA TGGATGGAGA GTTTATCCAC ATTAAGGTGC CTTCAGAAAG TGGAATTTCA 
GAGTTAATAT CCACAAGCAA TGAGCGCTTT AAACTTCATA TGGAAAATAC AAAACTGATA 
CCTGAGAAGG AAGTGGTACC TGGACAAAAG GGAATGGAAC TTTCAAAGGA GCCAGTTGAG 
TTAAGCAGTG GTGATTGGAA ACTTAATATA GTTCCCTGGG TTGGTGGAAG GATATTGTCT 
ATGACACATG TTCCCTCAGG AATTCAATGG CTTCATAGCA GGATAGATAT CAATGGTTAT 
GAGGAGTATA GTGGTACTGA GTACCGGTCA GCTGGATGTA CTGAGGAATA CAATGTCATT 
GAGAGGGATT TGGAGCATGC AGGAGAAGAA GAATCTCTGA TCCTAGAAGG TGATGTAGGT 
GGTGGACTGG TTCTTCGGCG TAAAATAAGT ATAGCTAAAG ACAATCAACG AGTTTTCCGG 
ATTGCCTCTA GCATTGAAGC CCGTAGTGTT GGTGCTGGTT CTGGTGGATT TTCAAGGCTG 
GTATGCTTAA GAGTCCATCC AACTTTCACT CTTTTGCATC CGACTGAATC GTTTGTCTCT 
TTCACGTCGA TTGATGGTTC AAAGCATGAA GTTTGGCCAG ATTCTGGAGA CCAAATATAT 
GAAGGGAACA ACCTTCCACA TGGGAAATGG ATGTTGGTGG ACAAGAGCCT CAATCTTCGG 
ATGGTAAACA GGTTCGATGT TAGTCAGGTC TTCAAATGTA TTATCCACTG GGACTGTGGA 
ACTGTTAACT TAGAGCTATG GTCTAAAGAG CGGCCTGTTT CCAAGGAATC TCCTCTGAAA 
ATAGAGCATG AATATGAAGT CACAAGTTTC CCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HGL1 ( NM_001338653.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001327915.1 Arabidopsis thaliana heteroglycan glucosidase 1 (HGL1), mRNA.

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CloneID	OAb37726
Clone ID Related Accession (Same CDS sequence)	NM_001338653.1
Accession Version	NM_001338653.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	heteroglycan glucosidase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074).

ATGGAGAGAT CATTTGTGAC ACAAGCAGCA GTATCATCGT CGTCATCACT CACTAGCAAA 
GCTCGAGCTT CTTCCTTCTT CTTCAATGGT CGCTCTCTTC CTTCTCTGAA CCCGATCTCT 
CCGTTTCTGT TCCTCCCCAC AAGTAGATCC TTCGCTCTCG TCGTCAAGAG CACTCTCGGA 
AGGAATGGAG ACAAATCTTT ACTTAGGATG ACTTTAAGTG GGGATAGCTC GGAAACTGTA 
GAGATGACTT CAACTGATAT GATTTTCGAG CCGATTTTGG AGCATGGAGT TTTCCGGTTT 
GATTGTTCTG TTGACCATAG GAAGGCTGCT TTTCCTAGTG TTTCATTTAA GAACAGCAAA 
GACCGGGAAG TTCCTATTGT GAGTCACATT GTTCCAGCAT ATATTCCTAC TTGTGGTTGT 
CTTCAGGACC AACAAGTTGT CACCTTTGAG TTTTCTCCTG GTACATCGTT TTATGGGACG 
GGAGAAGTTA GTGGTCAGTT GGAGAGAACA GGCAAACGGG TTTTTACTTG GAACACTGAT 
GCATGGGGAT ATGGTTCTGG AACCACTTCG TTATACCAGT CACATCCTTG GGTACTAGTT 
GTCCTTCCAA CTGGAGAAAC ACTAGGGGTT CTTGCTGATA CAACACGAAA GTGTGAGATT 
GATCTACGGA AAGAGGGAAT CATAAGAATT ATCTCCCCAG CATCATATCC AATAATCACA 
TTTGGTCCAT TCTCCTCGCC TACTGCTGTC TTGGAGTCCC TCTCGCACGC CATTGGAACT 
GTGTTTATGC CTCCGAAGTG GGCCTTAGGC TACCACCAAT GCCGTTGGAG TTATATGTCT 
GACAAGCGAG TAGCCGAGAT CGCTCAAACA TTTCGCGACA AGAAAATTCC TTCTGACGTG 
ATATGGATGG ATATTGACTA CATGGATGGT TTCCGTTGTT TCACATTCGA CAAGGAGCGT 
TTTCCGGATC CAAGTGCTTT GGCCAAAGAT CTCCATAGTA ATGGTTTCAA AGCTATCTGG 
ATGCTTGACC CTGGAATAAA GCAGGAGGAA GGTTATTATG TCTACGATAG TGGGAGTAAA 
AATGATGTTT GGATTAGCCG AGCAGATGGG AAGCCATTTA CTGGTGAAGT ATGGCCTGGG 
CCTTGCGTTT TCCCTGACTA TACAAATTCC AAAGCACGCT CTTGGTGGGC TAATTTGGTT 
AAGGAATTTG TTTCAAATGG TGTTGATGGT ATATGGAATG ATATGAATGA GCCAGCTGTT 
TTCAAAGTCG TGACAAAGAC GATGCCTGAG AACAATATTC ACCATGGAGA TGATGAGCTT 
GGAGGTGTTC AAAACCACTC ACACTACCAC AATGTCTATG GGATGCTGAT GGCAAGATCA 
ACTTACGAAG GGATGGAGCT AGCTGATAAG AATAAGCGAC CTTTTGTATT GACAAGGGCT 
GGATTCATAG GTAGCCAGAG ATATGCTGCG ACTTGGACAG GAGATAACCT TTCAAACTGG 
GAGCATCTAC ATATGTCTAT ATCCATGGTC CTTCAGCTGG GGCTTAGTGG TCAGCCCTTA 
TCAGGGCCAG ATATCGGTGG CTTTGCTGGC AATGCAACTC CGCGGCTATT TGGACGTTGG 
ATGGGTGTTG GAGCTATGTT TCCATTCTGT CGTGGCCATT CCGAGGCTGG CACTGATGAC 
CATGAACCAT GGTCCTTTGG AGAAGAGTGT GAGGAAGTCT GTCGTGCCGC ATTGAAGAGG 
CGTTACCAAC TCCTACCACA TTTTTACACC TTGTTTTACA TAGCCCATAC AACTGGTGCT 
CCAGTTGCAG CTCCAATCTT TTTCGCTGAT CCAATAGATT CCCGGCTAAG AGCTGTTGAA 
AATGGCTTTC TTTTGGGCCC ACTTCTTATA TATGCAAGCA CTTTGTCTAG TCAGGGATCA 
CATGAGCTGC AGCACATATT ACCGAGAGGA ATTTGGCATA GGTTTGATTT TGCAGATTCA 
CATCCGGATT TACCAACTTT ATATTTACAA GGGGGATCCA TCATATCATT GGCTCCTCCA 
CATCTGCATG TTGGGGAGTT TAGTCTGTCA GATGACTTGA CCCTACTTGT GTCATTAGAT 
GAAAATGGGA AAGCTAAAGG ACTCTTGTTC GAAGATGATG GAGATGGATA TGGCTACACT 
AAAGGAAGAT TTCTAGTTAC ACACTACATT GCTGAGCGGG ATTCTTCCAC TGTCACCGTC 
AAGGTTTCAA AAACTGAAGG AGACTGGCAG AGGCCAAACC GCCGTGTTCA TGTCCAATTA 
TTACTAGGAG GCGGTGCAAT GCTTGATGCT TGGGGAATGG ATGGAGAGTT TATCCACATT 
AAGGTGCCTT CAGAAAGTGG AATTTCAGAG TTAATATCCA CAAGCAATGA GCGCTTTAAA 
CTTCATATGG AAAATACAAA ACTGATACCT GAGAAGGAAG TGGTACCTGG ACAAAAGGGA 
ATGGAACTTT CAAAGGAGCC AGTTGAGTTA AGCAGTGGTG ATTGGAAACT TAATATAGTT 
CCCTGGGTTG GTGGAAGGAT ATTGTCTATG ACACATGTTC CCTCAGGAAT TCAATGGCTT 
CATAGCAGGA TAGATATCAA TGGTTATGAG GAGTATAGTG GTACTGAGTA CCGGTCAGCT 
GGATGTACTG AGGAATACAA TGTCATTGAG AGGGATTTGG AGCATGCAGG AGAAGAAGAA 
TCTCTGATCC TAGAAGGTGA TGTAGGTGGT GGACTGGTTC TTCGGCGTAA AATAAGTATA 
GCTAAAGACA ATCAACGAGT TTTCCGGATT GCCTCTAGCA TTGAAGCCCG TAGTGTTGGT 
GCTGGTTCTG GTGGATTTTC AAGGCTGGTA TGCTTAAGAG TCCATCCAAC TTTCACTCTT 
TTGCATCCGA CTGAATCGTT TGTCTCTTTC ACGTCGATTG ATGGTTCAAA GCATGAAGTT 
TGGCCAGATT CTGGAGACCA AATATATGAA GGGAACAACC TTCCACATGG GAAATGGATG 
TTGGTGGACA AGAGCCTCAA TCTTCGGATG GTAAACAGGT TCGATGTTAG TCAGGTCTTC 
AAATGTATTA TCCACTGGGA CTGTGGAACT GTTAACTTAG AGCTATGGTC TAAAGAGCGG 
CCTGTTTCCA AGGAATCTCC TCTGAAAATA GAGCATGAAT ATGAAGTCAC AAGTTTCCCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001327915.1
CDS	256..3438
Translation	MERSFVTQAAVSSSSSLTSKARASSFFFNGRSLPSLNPISPFLFLPTSRSFALVVKSTLGRNGDKSLLRMTLSGDSSETVEMTSTDMIFEPILEHGVFRFDCSVDHRKAAFPSVSFKNSKDREVPIVSHIVPAYIPTCGCLQDQQVVTFEFSPGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGSGTTSLYQSHPWVLVVLPTGETLGVLADTTRKCEIDLRKEGIIRIISPASYPIITFGPFSSPTAVLESLSHAIGTVFMPPKWALGYHQCRWSYMSDKRVAEIAQTFRDKKIPSDVIWMDIDYMDGFRCFTFDKERFPDPSALAKDLHSNGFKAIWMLDPGIKQEEGYYVYDSGSKNDVWISRADGKPFTGEVWPGPCVFPDYTNSKARSWWANLVKEFVSNGVDGIWNDMNEPAVFKVVTKTMPENNIHHGDDELGGVQNHSHYHNVYGMLMARSTYEGMELADKNKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPRLFGRWMGVGAMFPFCRGHSEAGTDDHEPWSFGEECEEVCRAALKRRYQLLPHFYTLFYIAHTTGAPVAAPIFFADPIDSRLRAVENGFLLGPLLIYASTLSSQGSHELQHILPRGIWHRFDFADSHPDLPTLYLQGGSIISLAPPHLHVGEFSLSDDLTLLVSLDENGKAKGLLFEDDGDGYGYTKGRFLVTHYIAERDSSTVTVKVSKTEGDWQRPNRRVHVQLLLGGGAMLDAWGMDGEFIHIKVPSESGISELISTSNERFKLHMENTKLIPEKEVVPGQKGMELSKEPVELSSGDWKLNIVPWVGGRILSMTHVPSGIQWLHSRIDINGYEEYSGTEYRSAGCTEEYNVIERDLEHAGEEESLILEGDVGGGLVLRRKISIAKDNQRVFRIASSIEARSVGAGSGGFSRLVCLRVHPTFTLLHPTESFVSFTSIDGSKHEVWPDSGDQIYEGNNLPHGKWMLVDKSLNLRMVNRFDVSQVFKCIIHWDCGTVNLELWSKERPVSKESPLKIEHEYEVTSFP

Target ORF information:

RefSeq Version	NM_001338653.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana heteroglycan glucosidase 1 (HGL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001338653.1

ORF Insert Sequence:

ATGGAGAGAT CATTTGTGAC ACAAGCAGCA GTATCATCGT CGTCATCACT CACTAGCAAA 
GCTCGAGCTT CTTCCTTCTT CTTCAATGGT CGCTCTCTTC CTTCTCTGAA CCCGATCTCT 
CCGTTTCTGT TCCTCCCCAC AAGTAGATCC TTCGCTCTCG TCGTCAAGAG CACTCTCGGA 
AGGAATGGAG ACAAATCTTT ACTTAGGATG ACTTTAAGTG GGGATAGCTC GGAAACTGTA 
GAGATGACTT CAACTGATAT GATTTTCGAG CCGATTTTGG AGCATGGAGT TTTCCGGTTT 
GATTGTTCTG TTGACCATAG GAAGGCTGCT TTTCCTAGTG TTTCATTTAA GAACAGCAAA 
GACCGGGAAG TTCCTATTGT GAGTCACATT GTTCCAGCAT ATATTCCTAC TTGTGGTTGT 
CTTCAGGACC AACAAGTTGT CACCTTTGAG TTTTCTCCTG GTACATCGTT TTATGGGACG 
GGAGAAGTTA GTGGTCAGTT GGAGAGAACA GGCAAACGGG TTTTTACTTG GAACACTGAT 
GCATGGGGAT ATGGTTCTGG AACCACTTCG TTATACCAGT CACATCCTTG GGTACTAGTT 
GTCCTTCCAA CTGGAGAAAC ACTAGGGGTT CTTGCTGATA CAACACGAAA GTGTGAGATT 
GATCTACGGA AAGAGGGAAT CATAAGAATT ATCTCCCCAG CATCATATCC AATAATCACA 
TTTGGTCCAT TCTCCTCGCC TACTGCTGTC TTGGAGTCCC TCTCGCACGC CATTGGAACT 
GTGTTTATGC CTCCGAAGTG GGCCTTAGGC TACCACCAAT GCCGTTGGAG TTATATGTCT 
GACAAGCGAG TAGCCGAGAT CGCTCAAACA TTTCGCGACA AGAAAATTCC TTCTGACGTG 
ATATGGATGG ATATTGACTA CATGGATGGT TTCCGTTGTT TCACATTCGA CAAGGAGCGT 
TTTCCGGATC CAAGTGCTTT GGCCAAAGAT CTCCATAGTA ATGGTTTCAA AGCTATCTGG 
ATGCTTGACC CTGGAATAAA GCAGGAGGAA GGTTATTATG TCTACGATAG TGGGAGTAAA 
AATGATGTTT GGATTAGCCG AGCAGATGGG AAGCCATTTA CTGGTGAAGT ATGGCCTGGG 
CCTTGCGTTT TCCCTGACTA TACAAATTCC AAAGCACGCT CTTGGTGGGC TAATTTGGTT 
AAGGAATTTG TTTCAAATGG TGTTGATGGT ATATGGAATG ATATGAATGA GCCAGCTGTT 
TTCAAAGTCG TGACAAAGAC GATGCCTGAG AACAATATTC ACCATGGAGA TGATGAGCTT 
GGAGGTGTTC AAAACCACTC ACACTACCAC AATGTCTATG GGATGCTGAT GGCAAGATCA 
ACTTACGAAG GGATGGAGCT AGCTGATAAG AATAAGCGAC CTTTTGTATT GACAAGGGCT 
GGATTCATAG GTAGCCAGAG ATATGCTGCG ACTTGGACAG GAGATAACCT TTCAAACTGG 
GAGCATCTAC ATATGTCTAT ATCCATGGTC CTTCAGCTGG GGCTTAGTGG TCAGCCCTTA 
TCAGGGCCAG ATATCGGTGG CTTTGCTGGC AATGCAACTC CGCGGCTATT TGGACGTTGG 
ATGGGTGTTG GAGCTATGTT TCCATTCTGT CGTGGCCATT CCGAGGCTGG CACTGATGAC 
CATGAACCAT GGTCCTTTGG AGAAGAGTGT GAGGAAGTCT GTCGTGCCGC ATTGAAGAGG 
CGTTACCAAC TCCTACCACA TTTTTACACC TTGTTTTACA TAGCCCATAC AACTGGTGCT 
CCAGTTGCAG CTCCAATCTT TTTCGCTGAT CCAATAGATT CCCGGCTAAG AGCTGTTGAA 
AATGGCTTTC TTTTGGGCCC ACTTCTTATA TATGCAAGCA CTTTGTCTAG TCAGGGATCA 
CATGAGCTGC AGCACATATT ACCGAGAGGA ATTTGGCATA GGTTTGATTT TGCAGATTCA 
CATCCGGATT TACCAACTTT ATATTTACAA GGGGGATCCA TCATATCATT GGCTCCTCCA 
CATCTGCATG TTGGGGAGTT TAGTCTGTCA GATGACTTGA CCCTACTTGT GTCATTAGAT 
GAAAATGGGA AAGCTAAAGG ACTCTTGTTC GAAGATGATG GAGATGGATA TGGCTACACT 
AAAGGAAGAT TTCTAGTTAC ACACTACATT GCTGAGCGGG ATTCTTCCAC TGTCACCGTC 
AAGGTTTCAA AAACTGAAGG AGACTGGCAG AGGCCAAACC GCCGTGTTCA TGTCCAATTA 
TTACTAGGAG GCGGTGCAAT GCTTGATGCT TGGGGAATGG ATGGAGAGTT TATCCACATT 
AAGGTGCCTT CAGAAAGTGG AATTTCAGAG TTAATATCCA CAAGCAATGA GCGCTTTAAA 
CTTCATATGG AAAATACAAA ACTGATACCT GAGAAGGAAG TGGTACCTGG ACAAAAGGGA 
ATGGAACTTT CAAAGGAGCC AGTTGAGTTA AGCAGTGGTG ATTGGAAACT TAATATAGTT 
CCCTGGGTTG GTGGAAGGAT ATTGTCTATG ACACATGTTC CCTCAGGAAT TCAATGGCTT 
CATAGCAGGA TAGATATCAA TGGTTATGAG GAGTATAGTG GTACTGAGTA CCGGTCAGCT 
GGATGTACTG AGGAATACAA TGTCATTGAG AGGGATTTGG AGCATGCAGG AGAAGAAGAA 
TCTCTGATCC TAGAAGGTGA TGTAGGTGGT GGACTGGTTC TTCGGCGTAA AATAAGTATA 
GCTAAAGACA ATCAACGAGT TTTCCGGATT GCCTCTAGCA TTGAAGCCCG TAGTGTTGGT 
GCTGGTTCTG GTGGATTTTC AAGGCTGGTA TGCTTAAGAG TCCATCCAAC TTTCACTCTT 
TTGCATCCGA CTGAATCGTT TGTCTCTTTC ACGTCGATTG ATGGTTCAAA GCATGAAGTT 
TGGCCAGATT CTGGAGACCA AATATATGAA GGGAACAACC TTCCACATGG GAAATGGATG 
TTGGTGGACA AGAGCCTCAA TCTTCGGATG GTAAACAGGT TCGATGTTAG TCAGGTCTTC 
AAATGTATTA TCCACTGGGA CTGTGGAACT GTTAACTTAG AGCTATGGTC TAAAGAGCGG 
CCTGTTTCCA AGGAATCTCC TCTGAAAATA GAGCATGAAT ATGAAGTCAC AAGTTTCCCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Nature408(6814)820-2(2000 Dec)
Salanoubat M,Lemcke K,Rieger M,Ansorge W,Unseld M,Fartmann B,Valle G,Bl?cker H,Perez-Alonso M,Obermaier B,Delseny M,Boutry M,Grivell LA,Mache R,Puigdom?nech P,De Simone V,Choisne N,Artiguenave F,Robert C,Brottier P,Wincker P,Cattolico L,Weissenbach J,Saurin W,Qu?tier F,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Benes V,Wurmbach E,Drzonek H,Erfle H,Jordan N,Bangert S,Wiedelmann R,Kranz H,Voss H,Holland R,Brandt P,Nyakatura G,Vezzi A,D'Angelo M,Pallavicini A,Toppo S,Simionati B,Conrad A,Hornischer K,Kauer G,L?hnert TH,Nordsiek G,Reichelt J,Scharfe M,Sch?n O,Bargues M,Terol J,Climent J,Navarro P,Collado C,Perez-Perez A,Ottenw?lder B,Duchemin D,Cooke R,Laudie M,Berger-Llauro C,Purnelle B,Masuy D,de Haan M,Maarse AC,Alcaraz JP,Cottet A,Casacuberta E,Monfort A,Argiriou A,flores M,Liguori R,Vitale D,Mannhaupt G,Haase D,Schoof H,Rudd S,Zaccaria P,Mewes HW,Mayer KF,Kaul S,Town CD,Koo HL,Tallon LJ,Jenkins J,Rooney T,Rizzo M,Walts A,Utterback T,Fujii CY,Shea TP,Creasy TH,Haas B,Maiti R,Wu D,Peterson J,Van Aken S,Pai G,Militscher J,Sellers P,Gill JE,Feldblyum TV,Preuss D,Lin X,Nierman WC,Salzberg SL,White O,Venter JC,Fraser CM,Kaneko T,Nakamura Y,Sato S,Kato T,Asamizu E,Sasamoto S,Kimura T,Idesawa K,Kawashima K,Kishida Y,Kiyokawa C,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Tabata S,,,

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HGL1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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