IBM1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	IBM1
Entrez Gene ID	819952
Full Name	Transcription factor jumonji (jmjC) domain-containing protein
Synonyms	increase in bonsai methylation 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001084652.2	NP_001078121.1	Transcription factor jumonji (jmjC) domain-containing protein
NM_111640.2	NP_187418.1	Transcription factor jumonji (jmjC) domain-containing protein
NM_001202909.1	NP_001189838.1	Transcription factor jumonji (jmjC) domain-containing protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Salanoubat M, Lemcke K, Rieger M, Ansorge W, Unseld M, Fartmann B, Valle G, Bl?cker H, Perez-Alonso M, Obermaier B, Delseny M, Boutry M, Grivell LA, Mache R, Puigdom?nech P, De Simone V, Choisne N, Artiguenave F, Robert C, Brottier P, Wincker P, Cattolico L, Weissenbach J, Saurin W, Qu?tier F, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Benes V, Wurmbach E, Drzonek H, Erfle H, Jordan N, Bangert S, Wiedelmann R, Kranz H, Voss H, Holland R, Brandt P, Nyakatura G, Vezzi A, D'Angelo M, Pallavicini A, Toppo S, Simionati B, Conrad A, Hornischer K, Kauer G, L?hnert TH, Nordsiek G, Reichelt J, Scharfe M, Sch?n O, Bargues M, Terol J, Climent J, Navarro P, Collado C, Perez-Perez A, Ottenw?lder B, Duchemin D, Cooke R, Laudie M, Berger-Llauro C, Purnelle B, Masuy D, de Haan M, Maarse AC, Alcaraz JP, Cottet A, Casacuberta E, Monfort A, Argiriou A, flores M, Liguori R, Vitale D, Mannhaupt G, Haase D, Schoof H, Rudd S, Zaccaria P, Mewes HW, Mayer KF, Kaul S, Town CD, Koo HL, Tallon LJ, Jenkins J, Rooney T, Rizzo M, Walts A, Utterback T, Fujii CY, Shea TP, Creasy TH, Haas B, Maiti R, Wu D, Peterson J, Van Aken S, Pai G, Militscher J, Sellers P, Gill JE, Feldblyum TV, Preuss D, Lin X, Nierman WC, Salzberg SL, White O, Venter JC, Fraser CM, Kaneko T, Nakamura Y, Sato S, Kato T, Asamizu E, Sasamoto S, Kimura T, Idesawa K, Kawashima K, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S, , ,
Nature408(6814)820-2(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The authors show that both ibm1 and edm2 mutants display defects in production of stomatal lineage cells, which is linked to DNA hypermethylation of the ERECTA family genes, including ER, ERL1 and ERL2 Stomatal phenotypes and DNA methylation levels of ER genes in ibm1 and edm2 mutants are restored by mutations in the genes encoding the histone methyltransferase KYP and DNA methyltransferase CMT3.
Title: Demethylation of ERECTA receptor genes by IBM1 histone demethylase affects stomatal development.

our results suggest a function for SG1 in the maintenance of genome integrity by regulating IBM1.
Title: SHOOT GROWTH1 maintains Arabidopsis epigenomes by regulating IBM1.

EDM2 promotes IBM1 distal polyadenylation and regulates genome DNA methylation
Title: Arabidopsis EDM2 promotes IBM1 distal polyadenylation and regulates genome DNA methylation patterns.

The results suggest that IBM1 regulates gene expression through two distinct pathways: direct association to protect genes from silencing by preventing the coupling of histone and DNA methylation, and indirect silencing of gene expression through RNA-directed DNA methylation-directed repression.
Title: IBM1, a JmjC domain-containing histone demethylase, is involved in the regulation of RNA-directed DNA methylation through the epigenetic control of RDR2 and DCL3 expression in Arabidopsis.

It was shown that the expression of the H3K9 demethylase IBM1 requires DNA methylation. Surprisingly, the regulatory methylated region is contained in an unusually large intron that is conserved in IBM1 orthologues.
Title: DNA methylation in an intron of the IBM1 histone demethylase gene stabilizes chromatin modification patterns.

The following IBM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IBM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb10518	NM_001084652.2 Latest version!	Arabidopsis thaliana Transcription factor jumonji (jmjC) domain-containing protein (IBM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OAb10519	NM_111640.2 Latest version!	Arabidopsis thaliana Transcription factor jumonji (jmjC) domain-containing protein (IBM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OAb32434	NM_001202909.1 Latest version!	Arabidopsis thaliana Transcription factor jumonji (jmjC) domain-containing protein (IBM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IBM1 ( NM_001084652.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001078121.1 Arabidopsis thaliana Transcription factor jumonji (jmjC) domain-containing protein (IBM1), mRNA.

Price & Availability↑

CloneID	OAb10518
Clone ID Related Accession (Same CDS sequence)	NM_001084652.2
Accession Version	NM_001084652.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Transcription factor jumonji (jmjC) domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Sep 12, 2016 this sequence version replaced NM_001084652.1.

ATGGATTCTG TGGAGGAAGA AGGTGTTGTT AGGGTTGAAG AAGAGAACGG ACGCGGTGGT 
CTCCGTAGGC ATCGGAGGGT TTCTACGAAG CTGGCGAATT ACGTAGATCC TCCGACTGAC 
GACGAAGAAG ATGGAGGACC CAAGCGAAAG GGCAAAAGGG GTGGAAATAG GGCTCCGAAA 
AAGACACCGA AGAAAGACGA GGAGATGCAA AAGAACGAAA TTGATGAAGC AAACCGAGTC 
ACTGGTCTGG TGAAGGAAAA AAGAGCCGCG ACTAAGATTC TGAATCGCAA AGACTCCATT 
ATTGAAGTAG GAGAAGCTTC TGGAAGCATG CCCAAGGAAG TAAAGGGAAT TAGGATTGGG 
AAAAGGAAGG GAGAAATCGA TGGTGAGATT CCAACTAAGC CGGGCAAGAA GCCAAAGACT 
ACAGTAGACC CGAGAATCAT TGGTTACAGA CCGGACAATA TGTGCCATCA ATGTCAAAAG 
AGCGATAGGA TTGTTGAACG GTGTCAGACC TGCAATAGTA AGCGTTATTG TCATCCATGC 
TTGGACACTT GGTACCCCCT TATAGCAAAA GAAGATGTTG CCAAGAAATG CATGTTCTGC 
TCTAGTACTT GCAATTGCAG AGCATGCTTG CGTCTAGATA CTAAATTGAA AGGGATAAAT 
TCGAACCTCA TAGTCAGCGA GGAAGAAAAG GTCCAAGCCT CTAAGTTTAT TCTGCAGAGC 
CTTCTCCCAC ATCTGAAAGG AATAAATGAT GAACAAGTTG CTGAGAAGGA AGTTGAGGCC 
AAAATATACG GACTGAAGTT TGAAGAGGTG AGGCCCCAAG ACGCTAAAGC TTTCCCTGAT 
GAAAGACTAT ACTGTGATAT CTGCAAGACT TCTATCTATG ATCTCCATAG GAATTGCAAG 
TCCTGTAGTT TTGATATCTG CCTTAGCTGT TGCCTTGAGA TCCGCAATGG AAAGGCTCTG 
GCATGTAAGG AGGATGTGTC TTGGAATTAT ATTAACCGAG GTTTAGAGTA TGAACATGGA 
CAAGAAGGAA AAGTGATTGA AAAGCCGGCG AATAAGCTAG ATGATAAGTT GAAAGATAAG 
CTGGATGGTA AGCCGGATGA TAAGCCGAAG GGTAAGCCAA AGGGTAGGCC AAAGGGTAAG 
CCGGATGATA AGCCGAAGGG TAAACTGAAG GGTAAGCAGG ATGATAAACC GGATGATAAG 
CCAGATGAGA AGCCGGTTAA TACAGACCAT ATGAAGTACC CTTCTCTGTG GAAAGCAAAT 
GAAGCTGGGA TCATTACTTG TTGTTGTGGT GCTGGGGAGT TAGTGCTGAA ACGACTACTT 
CCGGATGGTT GGATATCTGA GTTGGTCAAC AGAGTTGAAA AAACTGCTGA AGCTGGCGAG 
CTTTTGAATT TACCTGAAAC GGTTTTGGAG CGATGCCCTT GTTCTAACTC TGACAGACAT 
ATTGACATAG ACAGCTGTAA TTTGTTAAAA GCTGCTTGTC GAGAAGGTTC AGAAGACAAT 
TATCTTTACT CTCCAAGTGT ATGGGATGTT CAACAAGATG ATTTGAAGCA TTTTCAGCAC 
CATTGGGTAA AAGGCGAGCC TGTGATTGTG AGAAATGTGC TTGAAGCTAC ATCTGGTTTA 
AGCTGGGAAC CAATGGTAAT GCATCGTGCC TGCCGCCAGA TAAGCCATGT CCAGCATGGA 
TCACTTAAGG ATGTTGTTGC TGTTGATTGT TTGGACTTCT GTGAGGTAAA AGTTAATCTT 
CATGAATTTT TTACTGGATA CACGGATGGC CGATATGATC GAATGGGTTG GCCACTAGTT 
CTGAAACTGA AAGATTGGCC TCCAGCTAAA GTCTTCAAAG ATAACTTGCC ACGTCATGCT 
GAGGAGTTCT TATGTAGTTT GCCTTTGAAG CATTACACTC ATCCGGTTAA TGGACCTTTG 
AATCTCGCTG TCAAGCTTCC CCAAAATTGC TTGAAGCCAG ACATGGGACC AAAGACGTAC 
GTTGCTTCTG GATTTGCTCA AGAATTAGGC CGTGGAGATT CTGTTACTAA GCTCCACTGC 
GACATGTCTG ATGCGGTTAG TACCCATATG ATTTGTCTTT TCTCGTTTTC ATATCCATTT 
CTTTCTTCTA AGTCTGTCCA TTCTTATTGT TTTTCTTTGC ACATCTATAA TGATTTCAGA 
ACCTATGAAA ACATTAAAAT CAGATACCAT TTCCTAATCG GTCACTTAGA TTTTTTATTA 
CATTTAGGAT TCGTCTCCTC TTCTTTTCCA GATCAGTTCC TTAAGTTATT CTGTTTTTCT 
TTTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001078121.1
CDS	265..2553
Translation	MDSVEEEGVVRVEEENGRGGLRRHRRVSTKLANYVDPPTDDEEDGGPKRKGKRGGNRAPKKTPKKDEEMQKNEIDEANRVTGLVKEKRAATKILNRKDSIIEVGEASGSMPKEVKGIRIGKRKGEIDGEIPTKPGKKPKTTVDPRIIGYRPDNMCHQCQKSDRIVERCQTCNSKRYCHPCLDTWYPLIAKEDVAKKCMFCSSTCNCRACLRLDTKLKGINSNLIVSEEEKVQASKFILQSLLPHLKGINDEQVAEKEVEAKIYGLKFEEVRPQDAKAFPDERLYCDICKTSIYDLHRNCKSCSFDICLSCCLEIRNGKALACKEDVSWNYINRGLEYEHGQEGKVIEKPANKLDDKLKDKLDGKPDDKPKGKPKGRPKGKPDDKPKGKLKGKQDDKPDDKPDEKPVNTDHMKYPSLWKANEAGIITCCCGAGELVLKRLLPDGWISELVNRVEKTAEAGELLNLPETVLERCPCSNSDRHIDIDSCNLLKAACREGSEDNYLYSPSVWDVQQDDLKHFQHHWVKGEPVIVRNVLEATSGLSWEPMVMHRACRQISHVQHGSLKDVVAVDCLDFCEVKVNLHEFFTGYTDGRYDRMGWPLVLKLKDWPPAKVFKDNLPRHAEEFLCSLPLKHYTHPVNGPLNLAVKLPQNCLKPDMGPKTYVASGFAQELGRGDSVTKLHCDMSDAVSTHMICLFSFSYPFLSSKSVHSYCFSLHIYNDFRTYENIKIRYHFLIGHLDFLLHLGFVSSSFPDQFLKLFCFSFG

Target ORF information:

RefSeq Version	NM_001084652.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Transcription factor jumonji (jmjC) domain-containing protein (IBM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001084652.2

ORF Insert Sequence:

ATGGATTCTG TGGAGGAAGA AGGTGTTGTT AGGGTTGAAG AAGAGAACGG ACGCGGTGGT 
CTCCGTAGGC ATCGGAGGGT TTCTACGAAG CTGGCGAATT ACGTAGATCC TCCGACTGAC 
GACGAAGAAG ATGGAGGACC CAAGCGAAAG GGCAAAAGGG GTGGAAATAG GGCTCCGAAA 
AAGACACCGA AGAAAGACGA GGAGATGCAA AAGAACGAAA TTGATGAAGC AAACCGAGTC 
ACTGGTCTGG TGAAGGAAAA AAGAGCCGCG ACTAAGATTC TGAATCGCAA AGACTCCATT 
ATTGAAGTAG GAGAAGCTTC TGGAAGCATG CCCAAGGAAG TAAAGGGAAT TAGGATTGGG 
AAAAGGAAGG GAGAAATCGA TGGTGAGATT CCAACTAAGC CGGGCAAGAA GCCAAAGACT 
ACAGTAGACC CGAGAATCAT TGGTTACAGA CCGGACAATA TGTGCCATCA ATGTCAAAAG 
AGCGATAGGA TTGTTGAACG GTGTCAGACC TGCAATAGTA AGCGTTATTG TCATCCATGC 
TTGGACACTT GGTACCCCCT TATAGCAAAA GAAGATGTTG CCAAGAAATG CATGTTCTGC 
TCTAGTACTT GCAATTGCAG AGCATGCTTG CGTCTAGATA CTAAATTGAA AGGGATAAAT 
TCGAACCTCA TAGTCAGCGA GGAAGAAAAG GTCCAAGCCT CTAAGTTTAT TCTGCAGAGC 
CTTCTCCCAC ATCTGAAAGG AATAAATGAT GAACAAGTTG CTGAGAAGGA AGTTGAGGCC 
AAAATATACG GACTGAAGTT TGAAGAGGTG AGGCCCCAAG ACGCTAAAGC TTTCCCTGAT 
GAAAGACTAT ACTGTGATAT CTGCAAGACT TCTATCTATG ATCTCCATAG GAATTGCAAG 
TCCTGTAGTT TTGATATCTG CCTTAGCTGT TGCCTTGAGA TCCGCAATGG AAAGGCTCTG 
GCATGTAAGG AGGATGTGTC TTGGAATTAT ATTAACCGAG GTTTAGAGTA TGAACATGGA 
CAAGAAGGAA AAGTGATTGA AAAGCCGGCG AATAAGCTAG ATGATAAGTT GAAAGATAAG 
CTGGATGGTA AGCCGGATGA TAAGCCGAAG GGTAAGCCAA AGGGTAGGCC AAAGGGTAAG 
CCGGATGATA AGCCGAAGGG TAAACTGAAG GGTAAGCAGG ATGATAAACC GGATGATAAG 
CCAGATGAGA AGCCGGTTAA TACAGACCAT ATGAAGTACC CTTCTCTGTG GAAAGCAAAT 
GAAGCTGGGA TCATTACTTG TTGTTGTGGT GCTGGGGAGT TAGTGCTGAA ACGACTACTT 
CCGGATGGTT GGATATCTGA GTTGGTCAAC AGAGTTGAAA AAACTGCTGA AGCTGGCGAG 
CTTTTGAATT TACCTGAAAC GGTTTTGGAG CGATGCCCTT GTTCTAACTC TGACAGACAT 
ATTGACATAG ACAGCTGTAA TTTGTTAAAA GCTGCTTGTC GAGAAGGTTC AGAAGACAAT 
TATCTTTACT CTCCAAGTGT ATGGGATGTT CAACAAGATG ATTTGAAGCA TTTTCAGCAC 
CATTGGGTAA AAGGCGAGCC TGTGATTGTG AGAAATGTGC TTGAAGCTAC ATCTGGTTTA 
AGCTGGGAAC CAATGGTAAT GCATCGTGCC TGCCGCCAGA TAAGCCATGT CCAGCATGGA 
TCACTTAAGG ATGTTGTTGC TGTTGATTGT TTGGACTTCT GTGAGGTAAA AGTTAATCTT 
CATGAATTTT TTACTGGATA CACGGATGGC CGATATGATC GAATGGGTTG GCCACTAGTT 
CTGAAACTGA AAGATTGGCC TCCAGCTAAA GTCTTCAAAG ATAACTTGCC ACGTCATGCT 
GAGGAGTTCT TATGTAGTTT GCCTTTGAAG CATTACACTC ATCCGGTTAA TGGACCTTTG 
AATCTCGCTG TCAAGCTTCC CCAAAATTGC TTGAAGCCAG ACATGGGACC AAAGACGTAC 
GTTGCTTCTG GATTTGCTCA AGAATTAGGC CGTGGAGATT CTGTTACTAA GCTCCACTGC 
GACATGTCTG ATGCGGTTAG TACCCATATG ATTTGTCTTT TCTCGTTTTC ATATCCATTT 
CTTTCTTCTA AGTCTGTCCA TTCTTATTGT TTTTCTTTGC ACATCTATAA TGATTTCAGA 
ACCTATGAAA ACATTAAAAT CAGATACCAT TTCCTAATCG GTCACTTAGA TTTTTTATTA 
CATTTAGGAT TCGTCTCCTC TTCTTTTCCA GATCAGTTCC TTAAGTTATT CTGTTTTTCT 
TTTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IBM1 ( NM_111640.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_187418.1 Arabidopsis thaliana Transcription factor jumonji (jmjC) domain-containing protein (IBM1), mRNA.

Price & Availability↑

CloneID	OAb10519
Clone ID Related Accession (Same CDS sequence)	NM_111640.2
Accession Version	NM_111640.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Transcription factor jumonji (jmjC) domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Sep 12, 2016 this sequence version replaced NM_111640.1.

ATGGATTCTG TGGAGGAAGA AGGTGTTGTT AGGGTTGAAG AAGAGAACGG ACGCGGTGGT 
CTCCGTAGGC ATCGGAGGGT TTCTACGAAG CTGGCGAATT ACGTAGATCC TCCGACTGAC 
GACGAAGAAG ATGGAGGACC CAAGCGAAAG GGCAAAAGGG GTGGAAATAG GGCTCCGAAA 
AAGACACCGA AGAAAGACGA GGAGATGCAA AAGAACGAAA TTGATGAAGC AAACCGAGTC 
ACTGGTCTGG TGAAGGAAAA AAGAGCCGCG ACTAAGATTC TGAATCGCAA AGACTCCATT 
ATTGAAGTAG GAGAAGCTTC TGGAAGCATG CCCAAGGAAG TAAAGGGAAT TAGGATTGGG 
AAAAGGAAGG GAGAAATCGA TGGTGAGATT CCAACTAAGC CGGGCAAGAA GCCAAAGACT 
ACAGTAGACC CGAGAATCAT TGGTTACAGA CCGGACAATA TGTGCCATCA ATGTCAAAAG 
AGCGATAGGA TTGTTGAACG GTGTCAGACC TGCAATAGTA AGCGTTATTG TCATCCATGC 
TTGGACACTT GGTACCCCCT TATAGCAAAA GAAGATGTTG CCAAGAAATG CATGTTCTGC 
TCTAGTACTT GCAATTGCAG AGCATGCTTG CGTCTAGATA CTAAATTGAA AGGGATAAAT 
TCGAACCTCA TAGTCAGCGA GGAAGAAAAG GTCCAAGCCT CTAAGTTTAT TCTGCAGAGC 
CTTCTCCCAC ATCTGAAAGG AATAAATGAT GAACAAGTTG CTGAGAAGGA AGTTGAGGCC 
AAAATATACG GACTGAAGTT TGAAGAGGTG AGGCCCCAAG ACGCTAAAGC TTTCCCTGAT 
GAAAGACTAT ACTGTGATAT CTGCAAGACT TCTATCTATG ATCTCCATAG GAATTGCAAG 
TCCTGTAGTT TTGATATCTG CCTTAGCTGT TGCCTTGAGA TCCGCAATGG AAAGGCTCTG 
GCATGTAAGG AGGATGTGTC TTGGAATTAT ATTAACCGAG GTTTAGAGTA TGAACATGGA 
CAAGAAGGAA AAGTGATTGA AAAGCCGGCG AATAAGCTAG ATGATAAGTT GAAAGATAAG 
CTGGATGGTA AGCCGGATGA TAAGCCGAAG GGTAAGCCAA AGGGTAGGCC AAAGGGTAAG 
CCGGATGATA AGCCGAAGGG TAAACTGAAG GGTAAGCAGG ATGATAAACC GGATGATAAG 
CCAGATGAGA AGCCGGTTAA TACAGACCAT ATGAAGTACC CTTCTCTGTG GAAAGCAAAT 
GAAGCTGGGA TCATTACTTG TTGTTGTGGT GCTGGGGAGT TAGTGCTGAA ACGACTACTT 
CCGGATGGTT GGATATCTGA GTTGGTCAAC AGAGTTGAAA AAACTGCTGA AGCTGGCGAG 
CTTTTGAATT TACCTGAAAC GGTTTTGGAG CGATGCCCTT GTTCTAACTC TGACAGACAT 
ATTGACATAG ACAGCTGTAA TTTGTTAAAA GCTGCTTGTC GAGAAGGTTC AGAAGACAAT 
TATCTTTACT CTCCAAGTGT ATGGGATGTT CAACAAGATG ATTTGAAGCA TTTTCAGCAC 
CATTGGGTAA AAGGCGAGCC TGTGATTGTG AGAAATGTGC TTGAAGCTAC ATCTGGTTTA 
AGCTGGGAAC CAATGGTAAT GCATCGTGCC TGCCGCCAGA TAAGCCATGT CCAGCATGGA 
TCACTTAAGG ATGTTGTTGC TGTTGATTGT TTGGACTTCT GTGAGGTAAA AGTTAATCTT 
CATGAATTTT TTACTGGATA CACGGATGGC CGATATGATC GAATGGGTTG GCCACTAGTT 
CTGAAACTGA AAGATTGGCC TCCAGCTAAA GTCTTCAAAG ATAACTTGCC ACGTCATGCT 
GAGGAGTTCT TATGTAGTTT GCCTTTGAAG CATTACACTC ATCCGGTTAA TGGACCTTTG 
AATCTCGCTG TCAAGCTTCC CCAAAATTGC TTGAAGCCAG ACATGGGACC AAAGACGTAC 
GTTGCTTCTG GATTTGCTCA AGAATTAGGC CGTGGAGATT CTGTTACTAA GCTCCACTGC 
GACATGTCTG ATGCGGTGAA TATTTTGACG CACATATCTG AAGTGCCCAA TATGCAACCT 
GGAATTGGAA ATCTGAAAAA GAAGCATGCC GAACAAGATC TCAAGGAGCT GTATAGCTCA 
GTAGCTAACA AGGAGGAAAT GATGGAGATT CTTGAAAATT CCAGACAACA AGTCCAAAAT 
GTTGAAACTG ATGATGGAGC TCTTTGGGAC ATTTTCCGTA GAGAAGATAT TCCTAAATTA 
GAAAGTTACA TTGAGAAACA TCACAAGGAG TTCAGACATC TCTACTGCTG TCCTGTGTCT 
CAGGTTGTTC ATCCTATACA TGACCAGAAC TTCTATCTGA CGCGGTATCA TATAATGAAG 
CTGAAAGAAG AATATGGCAT TGAACCTTGG ACCTTCAATC AGAAGCTTGG TGATGCTGTT 
TTGATACCTG TAGGTTGCCC TCATCAAGTT AGAAATTTGA AGTCCTGCAA TAAGGTAGCG 
CTTGACTTCG TCTCACCTGA AAATGTCAGC GAGTGTTTAC GCTTGACAAA ACAGTATCGT 
CTACTTCCAC CAAATCATTT TGCAAAAGAA GACAAGTTAG GGGTTAAGAA GATGATAGTC 
CATGCAGTTG ATAAAGCTCT CAGAGACTTA AGTGGAGAGA AGTCTCCAGA ACCTGAAGAG 
AAGAAACAAA ATATGAGAGG GCCGAAGAAG GGGGCAGCCA AGGCAGTTGC CAAGGCTCTC 
AAAGACTTAT CTCCAAGTGA AAAGAAGTCT TCGGAAGCAG CTGAAGAGGA GATATCAAAT 
GGGATAGTCA ATGCAATCGA TAAGGGTCTC AAAGACTTAC CTCCAAGTGA AGAGAAGTCT 
TCCGAAGCCA AAGTGGAGAT ATCAAATGGG ATAGTCAGTG CAATGGATAA GGATCTCGAA 
CACATATCTT CAAGCGAAAA GAAGTCTACG GAAGAGGAAG GGGTGAAAAG ACCAAATATT 
GTGAGGACAT ATGAGCGGAG AAAAAAGCTG GGAAGTGAAG TAACCAACGC ATACATTGAC 
AGATTAGAAA TGGAGAAGAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_187418.1
CDS	265..3348
Translation	MDSVEEEGVVRVEEENGRGGLRRHRRVSTKLANYVDPPTDDEEDGGPKRKGKRGGNRAPKKTPKKDEEMQKNEIDEANRVTGLVKEKRAATKILNRKDSIIEVGEASGSMPKEVKGIRIGKRKGEIDGEIPTKPGKKPKTTVDPRIIGYRPDNMCHQCQKSDRIVERCQTCNSKRYCHPCLDTWYPLIAKEDVAKKCMFCSSTCNCRACLRLDTKLKGINSNLIVSEEEKVQASKFILQSLLPHLKGINDEQVAEKEVEAKIYGLKFEEVRPQDAKAFPDERLYCDICKTSIYDLHRNCKSCSFDICLSCCLEIRNGKALACKEDVSWNYINRGLEYEHGQEGKVIEKPANKLDDKLKDKLDGKPDDKPKGKPKGRPKGKPDDKPKGKLKGKQDDKPDDKPDEKPVNTDHMKYPSLWKANEAGIITCCCGAGELVLKRLLPDGWISELVNRVEKTAEAGELLNLPETVLERCPCSNSDRHIDIDSCNLLKAACREGSEDNYLYSPSVWDVQQDDLKHFQHHWVKGEPVIVRNVLEATSGLSWEPMVMHRACRQISHVQHGSLKDVVAVDCLDFCEVKVNLHEFFTGYTDGRYDRMGWPLVLKLKDWPPAKVFKDNLPRHAEEFLCSLPLKHYTHPVNGPLNLAVKLPQNCLKPDMGPKTYVASGFAQELGRGDSVTKLHCDMSDAVNILTHISEVPNMQPGIGNLKKKHAEQDLKELYSSVANKEEMMEILENSRQQVQNVETDDGALWDIFRREDIPKLESYIEKHHKEFRHLYCCPVSQVVHPIHDQNFYLTRYHIMKLKEEYGIEPWTFNQKLGDAVLIPVGCPHQVRNLKSCNKVALDFVSPENVSECLRLTKQYRLLPPNHFAKEDKLGVKKMIVHAVDKALRDLSGEKSPEPEEKKQNMRGPKKGAAKAVAKALKDLSPSEKKSSEAAEEEISNGIVNAIDKGLKDLPPSEEKSSEAKVEISNGIVSAMDKDLEHISSSEKKSTEEEGVKRPNIVRTYERRKKLGSEVTNAYIDRLEMEKM

Target ORF information:

RefSeq Version	NM_111640.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Transcription factor jumonji (jmjC) domain-containing protein (IBM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_111640.2

ORF Insert Sequence:

ATGGATTCTG TGGAGGAAGA AGGTGTTGTT AGGGTTGAAG AAGAGAACGG ACGCGGTGGT 
CTCCGTAGGC ATCGGAGGGT TTCTACGAAG CTGGCGAATT ACGTAGATCC TCCGACTGAC 
GACGAAGAAG ATGGAGGACC CAAGCGAAAG GGCAAAAGGG GTGGAAATAG GGCTCCGAAA 
AAGACACCGA AGAAAGACGA GGAGATGCAA AAGAACGAAA TTGATGAAGC AAACCGAGTC 
ACTGGTCTGG TGAAGGAAAA AAGAGCCGCG ACTAAGATTC TGAATCGCAA AGACTCCATT 
ATTGAAGTAG GAGAAGCTTC TGGAAGCATG CCCAAGGAAG TAAAGGGAAT TAGGATTGGG 
AAAAGGAAGG GAGAAATCGA TGGTGAGATT CCAACTAAGC CGGGCAAGAA GCCAAAGACT 
ACAGTAGACC CGAGAATCAT TGGTTACAGA CCGGACAATA TGTGCCATCA ATGTCAAAAG 
AGCGATAGGA TTGTTGAACG GTGTCAGACC TGCAATAGTA AGCGTTATTG TCATCCATGC 
TTGGACACTT GGTACCCCCT TATAGCAAAA GAAGATGTTG CCAAGAAATG CATGTTCTGC 
TCTAGTACTT GCAATTGCAG AGCATGCTTG CGTCTAGATA CTAAATTGAA AGGGATAAAT 
TCGAACCTCA TAGTCAGCGA GGAAGAAAAG GTCCAAGCCT CTAAGTTTAT TCTGCAGAGC 
CTTCTCCCAC ATCTGAAAGG AATAAATGAT GAACAAGTTG CTGAGAAGGA AGTTGAGGCC 
AAAATATACG GACTGAAGTT TGAAGAGGTG AGGCCCCAAG ACGCTAAAGC TTTCCCTGAT 
GAAAGACTAT ACTGTGATAT CTGCAAGACT TCTATCTATG ATCTCCATAG GAATTGCAAG 
TCCTGTAGTT TTGATATCTG CCTTAGCTGT TGCCTTGAGA TCCGCAATGG AAAGGCTCTG 
GCATGTAAGG AGGATGTGTC TTGGAATTAT ATTAACCGAG GTTTAGAGTA TGAACATGGA 
CAAGAAGGAA AAGTGATTGA AAAGCCGGCG AATAAGCTAG ATGATAAGTT GAAAGATAAG 
CTGGATGGTA AGCCGGATGA TAAGCCGAAG GGTAAGCCAA AGGGTAGGCC AAAGGGTAAG 
CCGGATGATA AGCCGAAGGG TAAACTGAAG GGTAAGCAGG ATGATAAACC GGATGATAAG 
CCAGATGAGA AGCCGGTTAA TACAGACCAT ATGAAGTACC CTTCTCTGTG GAAAGCAAAT 
GAAGCTGGGA TCATTACTTG TTGTTGTGGT GCTGGGGAGT TAGTGCTGAA ACGACTACTT 
CCGGATGGTT GGATATCTGA GTTGGTCAAC AGAGTTGAAA AAACTGCTGA AGCTGGCGAG 
CTTTTGAATT TACCTGAAAC GGTTTTGGAG CGATGCCCTT GTTCTAACTC TGACAGACAT 
ATTGACATAG ACAGCTGTAA TTTGTTAAAA GCTGCTTGTC GAGAAGGTTC AGAAGACAAT 
TATCTTTACT CTCCAAGTGT ATGGGATGTT CAACAAGATG ATTTGAAGCA TTTTCAGCAC 
CATTGGGTAA AAGGCGAGCC TGTGATTGTG AGAAATGTGC TTGAAGCTAC ATCTGGTTTA 
AGCTGGGAAC CAATGGTAAT GCATCGTGCC TGCCGCCAGA TAAGCCATGT CCAGCATGGA 
TCACTTAAGG ATGTTGTTGC TGTTGATTGT TTGGACTTCT GTGAGGTAAA AGTTAATCTT 
CATGAATTTT TTACTGGATA CACGGATGGC CGATATGATC GAATGGGTTG GCCACTAGTT 
CTGAAACTGA AAGATTGGCC TCCAGCTAAA GTCTTCAAAG ATAACTTGCC ACGTCATGCT 
GAGGAGTTCT TATGTAGTTT GCCTTTGAAG CATTACACTC ATCCGGTTAA TGGACCTTTG 
AATCTCGCTG TCAAGCTTCC CCAAAATTGC TTGAAGCCAG ACATGGGACC AAAGACGTAC 
GTTGCTTCTG GATTTGCTCA AGAATTAGGC CGTGGAGATT CTGTTACTAA GCTCCACTGC 
GACATGTCTG ATGCGGTGAA TATTTTGACG CACATATCTG AAGTGCCCAA TATGCAACCT 
GGAATTGGAA ATCTGAAAAA GAAGCATGCC GAACAAGATC TCAAGGAGCT GTATAGCTCA 
GTAGCTAACA AGGAGGAAAT GATGGAGATT CTTGAAAATT CCAGACAACA AGTCCAAAAT 
GTTGAAACTG ATGATGGAGC TCTTTGGGAC ATTTTCCGTA GAGAAGATAT TCCTAAATTA 
GAAAGTTACA TTGAGAAACA TCACAAGGAG TTCAGACATC TCTACTGCTG TCCTGTGTCT 
CAGGTTGTTC ATCCTATACA TGACCAGAAC TTCTATCTGA CGCGGTATCA TATAATGAAG 
CTGAAAGAAG AATATGGCAT TGAACCTTGG ACCTTCAATC AGAAGCTTGG TGATGCTGTT 
TTGATACCTG TAGGTTGCCC TCATCAAGTT AGAAATTTGA AGTCCTGCAA TAAGGTAGCG 
CTTGACTTCG TCTCACCTGA AAATGTCAGC GAGTGTTTAC GCTTGACAAA ACAGTATCGT 
CTACTTCCAC CAAATCATTT TGCAAAAGAA GACAAGTTAG GGGTTAAGAA GATGATAGTC 
CATGCAGTTG ATAAAGCTCT CAGAGACTTA AGTGGAGAGA AGTCTCCAGA ACCTGAAGAG 
AAGAAACAAA ATATGAGAGG GCCGAAGAAG GGGGCAGCCA AGGCAGTTGC CAAGGCTCTC 
AAAGACTTAT CTCCAAGTGA AAAGAAGTCT TCGGAAGCAG CTGAAGAGGA GATATCAAAT 
GGGATAGTCA ATGCAATCGA TAAGGGTCTC AAAGACTTAC CTCCAAGTGA AGAGAAGTCT 
TCCGAAGCCA AAGTGGAGAT ATCAAATGGG ATAGTCAGTG CAATGGATAA GGATCTCGAA 
CACATATCTT CAAGCGAAAA GAAGTCTACG GAAGAGGAAG GGGTGAAAAG ACCAAATATT 
GTGAGGACAT ATGAGCGGAG AAAAAAGCTG GGAAGTGAAG TAACCAACGC ATACATTGAC 
AGATTAGAAA TGGAGAAGAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IBM1 ( NM_001202909.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001189838.1 Arabidopsis thaliana Transcription factor jumonji (jmjC) domain-containing protein (IBM1), mRNA.

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CloneID	OAb32434
Clone ID Related Accession (Same CDS sequence)	NM_001202909.1
Accession Version	NM_001202909.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Transcription factor jumonji (jmjC) domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074).

ATGGATTCTG TGGAGGAAGA AGGTGTTGTT AGGGTTGAAG AAGAGAACGG ACGCGGTGGT 
CTCCGTAGGC ATCGGAGGGT TTCTACGAAG CTGGCGAATT ACGTAGATCC TCCGACTGAC 
GACGAAGAAG ATGGAGGACC CAAGCGAAAG GGCAAAAGGG GTGGAAATAG GGCTCCGAAA 
AAGACACCGA AGAAAGACGA GGAGATGCAA AAGAACGAAA TTGATGAAGC AAACCGAGTC 
ACTGGTCTGG TGAAGGAAAA AAGAGCCGCG ACTAAGATTC TGAATCGCAA AGACTCCATT 
ATTGAAGTAG GAGAAGCTTC TGGAAGCATG CCCAAGGAAG TAAAGGGAAT TAGGATTGGG 
AAAAGGAAGG GAGAAATCGA TGGTGAGATT CCAACTAAGC CGGGCAAGAA GCCAAAGACT 
ACAGTAGACC CGAGAATCAT TGGTTACAGA CCGGACAATA TGTGCCATCA ATGTCAAAAG 
AGCGATAGGA TTGTTGAACG GTGTCAGACC TGCAATAGTA AGCGTTATTG TCATCCATGC 
TTGGACACTT GGTACCCCCT TATAGCAAAA GAAGATGTTG CCAAGAAATG CATGTTCTGC 
TCTAGTACTT GCAATTGCAG AGCATGCTTG CGTCTAGATA CTAAATTGAA AGGGATAAAT 
TCGAACCTCA TAGTCAGCGA GGAAGAAAAG GTCCAAGCCT CTAAGTTTAT TCTGCAGAGC 
CTTCTCCCAC ATCTGAAAGG AATAAATGAT GAACAAGTTG CTGAGAAGGA AGTTGAGGCC 
AAAATATACG GACTGAAGTT TGAAGAGGTG AGGCCCCAAG ACGCTAAAGC TTTCCCTGAT 
GAAAGACTAT ACTGTGATAT CTGCAAGACT TCTATCTATG ATCTCCATAG GAATTGCAAG 
TCCTGTAGTT TTGATATCTG CCTTAGCTGT TGCCTTGAGA TCCGCAATGG AAAGGCTCTG 
GCATGTAAGG AGGATGTGTC TTGGAATTAT ATTAACCGAG GTTTAGAGTA TGAACATGGA 
CAAGAAGGAA AAGTGATTGA AAAGCCGGCG AATAAGCTAG ATGATAAGTT GAAAGATAAG 
CTGGATGGTA AGCCGGATGA TAAGCCGAAG GGTAAGCCAA AGGGTAGGCC AAAGGGTAAG 
CCGGATGATA AGCCGAAGGG TAAACTGAAG GGTAAGCAGG ATGATAAACC GGATGATAAG 
CCAGATGAGA AGCCGGTTAA TACAGACCAT ATGAAGTACC CTTCTCTGTG GAAAGCAAAT 
GAAGCTGGGA TCATTACTTG TTGTTGTGGT GCTGGGGAGT TAGTGCTGAA ACGACTACTT 
CCGGATGGTT GGATATCTGA GTTGGTCAAC AGAGTTGAAA AAACTGCTGA AGCTGGCGAG 
CTTTTGAATT TACCTGAAAC GGTTTTGGAG CGATGCCCTT GTTCTAACTC TGACAGACAT 
ATTGACATAG ACAGCTGTAA TTTGTTAAAA GCTGCTTGTC GAGAAGGTTC AGAAGACAAT 
TATCTTTACT CTCCAAGTGT ATGGGATGTT CAACAAGATG ATTTGAAGCA TTTTCAGCAC 
CATTGGGTAA AAGGCGAGCC TGTGATTGTG AGAAATGTGC TTGAAGCTAC ATCTGGTTTA 
AGCTGGGAAC CAATGGTAAT GCATCGTGCC TGCCGCCAGA TAAGCCATGT CCAGCATGGA 
TCACTTAAGG ATGTTGTTGC TGTTGATTGT TTGGACTTCT GTGAGGTAAA AGTTAATCTT 
CATGAATTTT TTACTGGATA CACGGATGGC CGATATGATC GAATGGGTTG GCCACTAGTT 
CTGAAACTGA AAGATTGGCC TCCAGCTAAA GTCTTCAAAG ATAACTTGCC ACGTCATGCT 
GAGGAGTTCT TATGTAGTTT GCCTTTGAAG CATTACACTC ATCCGGTTAA TGGACCTTTG 
AATCTCGCTG TCAAGCTTCC CCAAAATTGC TTGAAGCCAG ACATGGGACC AAAGACGTAC 
GTTGCTTCTG GATTTGCTCA AGAATTAGGC CGTGGAGATT CTGTTACTAA GCTCCACTGC 
GACATGTCTG ATGCGGACCT ATGGACCAAG CTTTATCCAT TCAATGTGAG TCCATCAATA 
GCTTTCTTAG TCCTTCATAG GCCTGGCATT CGGTTTTTGG TTCTGTTAAT GCCCAATATG 
CAACCTGGAA TTGGAAATCT GAAAAAGAAG CATGCCGAAC AAGATCTCAA GGAGCTGTAT 
AGCTCAGTAG CTAACAAGGA GGAAATGATG GAGATTCTTG AAAATTCCAG ACAACAAGTC 
CAAAATGTTG AAACTGATGA TGGAGCTCTT TGGGACATTT TCCGTAGAGA AGATATTCCT 
AAATTAGAAA GTTACATTGA GAAACATCAC AAGGAGTTCA GACATCTCTA CTGCTGTCCT 
GTGTCTCAGG TTGTTCATCC TATACATGAC CAGAACTTCT ATCTGACGCG GTATCATATA 
ATGAAGCTGA AAGAAGAATA TGGCATTGAA CCTTGGACCT TCAATCAGAA GCTTGGTGAT 
GCTGTTTTGA TACCTGTAGG TTGCCCTCAT CAAGTTAGAA ATTTGAAGTC CTGCAATAAG 
GTAGCGCTTG ACTTCGTCTC ACCTGAAAAT GTCAGCGAGT GTTTACGCTT GACAAAACAG 
TATCGTCTAC TTCCACCAAA TCATTTTGCA AAAGAAGACA AGTTAGGGGT TAAGAAGATG 
ATAGTCCATG CAGTTGATAA AGCTCTCAGA GACTTAAGTG GAGAGAAGTC TCCAGAACCT 
GAAGAGAAGA AACAAAATAT GAGAGGGCCG AAGAAGGGGG CAGCCAAGGC AGTTGCCAAG 
GCTCTCAAAG ACTTATCTCC AAGTGAAAAG AAGTCTTCGG AAGCAGCTGA AGAGGAGATA 
TCAAATGGGA TAGTCAATGC AATCGATAAG GGTCTCAAAG ACTTACCTCC AAGTGAAGAG 
AAGTCTTCCG AAGCCAAAGT GGAGATATCA AATGGGATAG TCAGTGCAAT GGATAAGGAT 
CTCGAACACA TATCTTCAAG CGAAAAGAAG TCTACGGAAG AGGAAGGGGT GAAAAGACCA 
AATATTGTGA GGACATATGA GCGGAGAAAA AAGCTGGGAA GTGAAGTAAC CAACGCATAC 
ATTGACAGAT TAGAAATGGA GAAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189838.1
CDS	80..3229
Translation	MDSVEEEGVVRVEEENGRGGLRRHRRVSTKLANYVDPPTDDEEDGGPKRKGKRGGNRAPKKTPKKDEEMQKNEIDEANRVTGLVKEKRAATKILNRKDSIIEVGEASGSMPKEVKGIRIGKRKGEIDGEIPTKPGKKPKTTVDPRIIGYRPDNMCHQCQKSDRIVERCQTCNSKRYCHPCLDTWYPLIAKEDVAKKCMFCSSTCNCRACLRLDTKLKGINSNLIVSEEEKVQASKFILQSLLPHLKGINDEQVAEKEVEAKIYGLKFEEVRPQDAKAFPDERLYCDICKTSIYDLHRNCKSCSFDICLSCCLEIRNGKALACKEDVSWNYINRGLEYEHGQEGKVIEKPANKLDDKLKDKLDGKPDDKPKGKPKGRPKGKPDDKPKGKLKGKQDDKPDDKPDEKPVNTDHMKYPSLWKANEAGIITCCCGAGELVLKRLLPDGWISELVNRVEKTAEAGELLNLPETVLERCPCSNSDRHIDIDSCNLLKAACREGSEDNYLYSPSVWDVQQDDLKHFQHHWVKGEPVIVRNVLEATSGLSWEPMVMHRACRQISHVQHGSLKDVVAVDCLDFCEVKVNLHEFFTGYTDGRYDRMGWPLVLKLKDWPPAKVFKDNLPRHAEEFLCSLPLKHYTHPVNGPLNLAVKLPQNCLKPDMGPKTYVASGFAQELGRGDSVTKLHCDMSDADLWTKLYPFNVSPSIAFLVLHRPGIRFLVLLMPNMQPGIGNLKKKHAEQDLKELYSSVANKEEMMEILENSRQQVQNVETDDGALWDIFRREDIPKLESYIEKHHKEFRHLYCCPVSQVVHPIHDQNFYLTRYHIMKLKEEYGIEPWTFNQKLGDAVLIPVGCPHQVRNLKSCNKVALDFVSPENVSECLRLTKQYRLLPPNHFAKEDKLGVKKMIVHAVDKALRDLSGEKSPEPEEKKQNMRGPKKGAAKAVAKALKDLSPSEKKSSEAAEEEISNGIVNAIDKGLKDLPPSEEKSSEAKVEISNGIVSAMDKDLEHISSSEKKSTEEEGVKRPNIVRTYERRKKLGSEVTNAYIDRLEMEKM

Target ORF information:

RefSeq Version	NM_001202909.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Transcription factor jumonji (jmjC) domain-containing protein (IBM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202909.1

ORF Insert Sequence:

ATGGATTCTG TGGAGGAAGA AGGTGTTGTT AGGGTTGAAG AAGAGAACGG ACGCGGTGGT 
CTCCGTAGGC ATCGGAGGGT TTCTACGAAG CTGGCGAATT ACGTAGATCC TCCGACTGAC 
GACGAAGAAG ATGGAGGACC CAAGCGAAAG GGCAAAAGGG GTGGAAATAG GGCTCCGAAA 
AAGACACCGA AGAAAGACGA GGAGATGCAA AAGAACGAAA TTGATGAAGC AAACCGAGTC 
ACTGGTCTGG TGAAGGAAAA AAGAGCCGCG ACTAAGATTC TGAATCGCAA AGACTCCATT 
ATTGAAGTAG GAGAAGCTTC TGGAAGCATG CCCAAGGAAG TAAAGGGAAT TAGGATTGGG 
AAAAGGAAGG GAGAAATCGA TGGTGAGATT CCAACTAAGC CGGGCAAGAA GCCAAAGACT 
ACAGTAGACC CGAGAATCAT TGGTTACAGA CCGGACAATA TGTGCCATCA ATGTCAAAAG 
AGCGATAGGA TTGTTGAACG GTGTCAGACC TGCAATAGTA AGCGTTATTG TCATCCATGC 
TTGGACACTT GGTACCCCCT TATAGCAAAA GAAGATGTTG CCAAGAAATG CATGTTCTGC 
TCTAGTACTT GCAATTGCAG AGCATGCTTG CGTCTAGATA CTAAATTGAA AGGGATAAAT 
TCGAACCTCA TAGTCAGCGA GGAAGAAAAG GTCCAAGCCT CTAAGTTTAT TCTGCAGAGC 
CTTCTCCCAC ATCTGAAAGG AATAAATGAT GAACAAGTTG CTGAGAAGGA AGTTGAGGCC 
AAAATATACG GACTGAAGTT TGAAGAGGTG AGGCCCCAAG ACGCTAAAGC TTTCCCTGAT 
GAAAGACTAT ACTGTGATAT CTGCAAGACT TCTATCTATG ATCTCCATAG GAATTGCAAG 
TCCTGTAGTT TTGATATCTG CCTTAGCTGT TGCCTTGAGA TCCGCAATGG AAAGGCTCTG 
GCATGTAAGG AGGATGTGTC TTGGAATTAT ATTAACCGAG GTTTAGAGTA TGAACATGGA 
CAAGAAGGAA AAGTGATTGA AAAGCCGGCG AATAAGCTAG ATGATAAGTT GAAAGATAAG 
CTGGATGGTA AGCCGGATGA TAAGCCGAAG GGTAAGCCAA AGGGTAGGCC AAAGGGTAAG 
CCGGATGATA AGCCGAAGGG TAAACTGAAG GGTAAGCAGG ATGATAAACC GGATGATAAG 
CCAGATGAGA AGCCGGTTAA TACAGACCAT ATGAAGTACC CTTCTCTGTG GAAAGCAAAT 
GAAGCTGGGA TCATTACTTG TTGTTGTGGT GCTGGGGAGT TAGTGCTGAA ACGACTACTT 
CCGGATGGTT GGATATCTGA GTTGGTCAAC AGAGTTGAAA AAACTGCTGA AGCTGGCGAG 
CTTTTGAATT TACCTGAAAC GGTTTTGGAG CGATGCCCTT GTTCTAACTC TGACAGACAT 
ATTGACATAG ACAGCTGTAA TTTGTTAAAA GCTGCTTGTC GAGAAGGTTC AGAAGACAAT 
TATCTTTACT CTCCAAGTGT ATGGGATGTT CAACAAGATG ATTTGAAGCA TTTTCAGCAC 
CATTGGGTAA AAGGCGAGCC TGTGATTGTG AGAAATGTGC TTGAAGCTAC ATCTGGTTTA 
AGCTGGGAAC CAATGGTAAT GCATCGTGCC TGCCGCCAGA TAAGCCATGT CCAGCATGGA 
TCACTTAAGG ATGTTGTTGC TGTTGATTGT TTGGACTTCT GTGAGGTAAA AGTTAATCTT 
CATGAATTTT TTACTGGATA CACGGATGGC CGATATGATC GAATGGGTTG GCCACTAGTT 
CTGAAACTGA AAGATTGGCC TCCAGCTAAA GTCTTCAAAG ATAACTTGCC ACGTCATGCT 
GAGGAGTTCT TATGTAGTTT GCCTTTGAAG CATTACACTC ATCCGGTTAA TGGACCTTTG 
AATCTCGCTG TCAAGCTTCC CCAAAATTGC TTGAAGCCAG ACATGGGACC AAAGACGTAC 
GTTGCTTCTG GATTTGCTCA AGAATTAGGC CGTGGAGATT CTGTTACTAA GCTCCACTGC 
GACATGTCTG ATGCGGACCT ATGGACCAAG CTTTATCCAT TCAATGTGAG TCCATCAATA 
GCTTTCTTAG TCCTTCATAG GCCTGGCATT CGGTTTTTGG TTCTGTTAAT GCCCAATATG 
CAACCTGGAA TTGGAAATCT GAAAAAGAAG CATGCCGAAC AAGATCTCAA GGAGCTGTAT 
AGCTCAGTAG CTAACAAGGA GGAAATGATG GAGATTCTTG AAAATTCCAG ACAACAAGTC 
CAAAATGTTG AAACTGATGA TGGAGCTCTT TGGGACATTT TCCGTAGAGA AGATATTCCT 
AAATTAGAAA GTTACATTGA GAAACATCAC AAGGAGTTCA GACATCTCTA CTGCTGTCCT 
GTGTCTCAGG TTGTTCATCC TATACATGAC CAGAACTTCT ATCTGACGCG GTATCATATA 
ATGAAGCTGA AAGAAGAATA TGGCATTGAA CCTTGGACCT TCAATCAGAA GCTTGGTGAT 
GCTGTTTTGA TACCTGTAGG TTGCCCTCAT CAAGTTAGAA ATTTGAAGTC CTGCAATAAG 
GTAGCGCTTG ACTTCGTCTC ACCTGAAAAT GTCAGCGAGT GTTTACGCTT GACAAAACAG 
TATCGTCTAC TTCCACCAAA TCATTTTGCA AAAGAAGACA AGTTAGGGGT TAAGAAGATG 
ATAGTCCATG CAGTTGATAA AGCTCTCAGA GACTTAAGTG GAGAGAAGTC TCCAGAACCT 
GAAGAGAAGA AACAAAATAT GAGAGGGCCG AAGAAGGGGG CAGCCAAGGC AGTTGCCAAG 
GCTCTCAAAG ACTTATCTCC AAGTGAAAAG AAGTCTTCGG AAGCAGCTGA AGAGGAGATA 
TCAAATGGGA TAGTCAATGC AATCGATAAG GGTCTCAAAG ACTTACCTCC AAGTGAAGAG 
AAGTCTTCCG AAGCCAAAGT GGAGATATCA AATGGGATAG TCAGTGCAAT GGATAAGGAT 
CTCGAACACA TATCTTCAAG CGAAAAGAAG TCTACGGAAG AGGAAGGGGT GAAAAGACCA 
AATATTGTGA GGACATATGA GCGGAGAAAA AAGCTGGGAA GTGAAGTAAC CAACGCATAC 
ATTGACAGAT TAGAAATGGA GAAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Nature408(6814)820-2(2000 Dec)
Salanoubat M,Lemcke K,Rieger M,Ansorge W,Unseld M,Fartmann B,Valle G,Bl?cker H,Perez-Alonso M,Obermaier B,Delseny M,Boutry M,Grivell LA,Mache R,Puigdom?nech P,De Simone V,Choisne N,Artiguenave F,Robert C,Brottier P,Wincker P,Cattolico L,Weissenbach J,Saurin W,Qu?tier F,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Benes V,Wurmbach E,Drzonek H,Erfle H,Jordan N,Bangert S,Wiedelmann R,Kranz H,Voss H,Holland R,Brandt P,Nyakatura G,Vezzi A,D'Angelo M,Pallavicini A,Toppo S,Simionati B,Conrad A,Hornischer K,Kauer G,L?hnert TH,Nordsiek G,Reichelt J,Scharfe M,Sch?n O,Bargues M,Terol J,Climent J,Navarro P,Collado C,Perez-Perez A,Ottenw?lder B,Duchemin D,Cooke R,Laudie M,Berger-Llauro C,Purnelle B,Masuy D,de Haan M,Maarse AC,Alcaraz JP,Cottet A,Casacuberta E,Monfort A,Argiriou A,flores M,Liguori R,Vitale D,Mannhaupt G,Haase D,Schoof H,Rudd S,Zaccaria P,Mewes HW,Mayer KF,Kaul S,Town CD,Koo HL,Tallon LJ,Jenkins J,Rooney T,Rizzo M,Walts A,Utterback T,Fujii CY,Shea TP,Creasy TH,Haas B,Maiti R,Wu D,Peterson J,Van Aken S,Pai G,Militscher J,Sellers P,Gill JE,Feldblyum TV,Preuss D,Lin X,Nierman WC,Salzberg SL,White O,Venter JC,Fraser CM,Kaneko T,Nakamura Y,Sato S,Kato T,Asamizu E,Sasamoto S,Kimura T,Idesawa K,Kawashima K,Kishida Y,Kiyokawa C,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Tabata S,,,

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IBM1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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