AFO cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AFO
Entrez Gene ID	819127
Full Name	Plant-specific transcription factor YABBY family protein
Synonyms	ABNORMAL FLORAL ORGANS,ABNORMAL FLORAL ORGANS PROTEIN,F4L23.30,FIL,FILAMENTOUS FLOWER,YAB1,YABBY1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001337117.1	NP_001323472.1	Plant-specific transcription factor YABBY family protein
NM_130082.4	NP_566037.1	Plant-specific transcription factor YABBY family protein

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	AFO	NP_566037.1
Arabidopsis thaliana (thale cress)	YAB3	NP_567154.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Lin X, Kaul S, Rounsley S, Shea TP, Benito MI, Town CD, Fujii CY, Mason T, Bowman CL, Barnstead M, Feldblyum TV, Buell CR, Ketchum KA, Lee J, Ronning CM, Koo HL, Moffat KS, Cronin LA, Shen M, Pai G, Van Aken S, Umayam L, Tallon LJ, Gill JE, Adams MD, Carrera AJ, Creasy TH, Goodman HM, Somerville CR, Copenhaver GP, Preuss D, Nierman WC, White O, Eisen JA, Salzberg SL, Fraser CM, Venter JC
Nature402(6763)761-8(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

BREVIPEDICELLUS (BP) encodes a class-I KNOTTED1-like homeobox (KNOX) transcription factor. FIL acts to oppose KNOX1 gene function through a complex regulatory network that involves changes in secondary metabolites and auxin.
Title: A novel Filamentous Flower mutant suppresses brevipedicellus developmental defects and modulates glucosinolate and auxin levels.

JAZ3 interacts with YABs to attenuate their transcriptional function.
Title: FILAMENTOUS FLOWER Is a Direct Target of JAZ3 and Modulates Responses to Jasmonate.

FIL functions as a transcriptional activator in plants and that this activity is involved in leaf patterning.
Title: FILAMENTOUS FLOWER controls lateral organ development by acting as both an activator and a repressor.

Mutation of these YAB and KNOX1 genes together causes nearly complete suppression of bop1 bop2 ectopic organ outgrowth at the morphological and cellular levels.
Title: Control of Arabidopsis leaf morphogenesis through regulation of the YABBY and KNOX families of transcription factors.

fil-9 allele mutant flowers show a wide range of organ morphological and identity defects.
Title: A novel allele of FILAMENTOUS FLOWER reveals new insights on the link between inflorescence and floral meristem organization and flower morphogenesis.

The following AFO gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFO cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb09818	NM_130082.4 Latest version!	Arabidopsis thaliana Plant-specific transcription factor YABBY family protein (AFO), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OAb36634	NM_001337117.1 Latest version!	Arabidopsis thaliana Plant-specific transcription factor YABBY family protein (AFO), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AFO ( NM_130082.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_566037.1 Arabidopsis thaliana Plant-specific transcription factor YABBY family protein (AFO), mRNA.

Price & Availability↑

CloneID	OAb09818
Clone ID Related Accession (Same CDS sequence)	NM_130082.4
Accession Version	NM_130082.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Plant-specific transcription factor YABBY family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071). On Sep 12, 2016 this sequence version replaced NM_130082.3.

ATGTCTATGT CGTCTATGTC CTCCCCTTCC TCAGCTGTTT GTTCACCGGA CCACTTCTCT 
CCTTCCGACC ATCTCTGCTA TGTCCAATGC AACTTTTGCC AAACCATCCT TGCGGTTAAT 
GTTCCTTACA CAAGCTTGTT CAAGACCGTA ACTGTCCGAT GTGGTTGCTG TACCAATCTC 
CTTTCGGTGA ACATGAGATC ATATGTCCTC CCAGCTTCTA ACCAGCTCCA GCTCCAGCTC 
GGTCCTCACT CTTACTTCAA TCCCCAGGAT ATTCTGGAGG AGCTGAGAGA TGCACCGTCT 
AACATGAATA TGATGATGAT GAATCAACAT CCTACTATGA ATGACATACC ATCTTTCATG 
GATCTTCATC AACAACATGA GATTCCTAAA GCACCACCCG TTAACCGCCC TCCAGAGAAA 
AGACAGAGAG TCCCATCCGC ATATAACCGA TTCATCAAGG AGGAGATCCA ACGTATCAAA 
GCTGGTAATC CTGATATAAG CCACAGAGAA GCCTTTAGTG CTGCTGCCAA GAATTGGGCC 
CACTTCCCCC ACATACACTT CGGGCTCGTG CCAGACAATC AACCCGTGAA GAAAACCAAC 
ATGCCCCAAC AGGAGGGAGA GGATAACATG GTGATGAAAG AAGGGTTCTA CGCTCCTGCA 
GCTGCTAACG TTGGTGTGAC TCCTTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_566037.1
CDS	228..917
Translation	MSMSSMSSPSSAVCSPDHFSPSDHLCYVQCNFCQTILAVNVPYTSLFKTVTVRCGCCTNLLSVNMRSYVLPASNQLQLQLGPHSYFNPQDILEELRDAPSNMNMMMMNQHPTMNDIPSFMDLHQQHEIPKAPPVNRPPEKRQRVPSAYNRFIKEEIQRIKAGNPDISHREAFSAAAKNWAHFPHIHFGLVPDNQPVKKTNMPQQEGEDNMVMKEGFYAPAAANVGVTPY

Target ORF information:

RefSeq Version	NM_130082.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Plant-specific transcription factor YABBY family protein (AFO), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130082.4

ORF Insert Sequence:

ATGTCTATGT CGTCTATGTC CTCCCCTTCC TCAGCTGTTT GTTCACCGGA CCACTTCTCT 
CCTTCCGACC ATCTCTGCTA TGTCCAATGC AACTTTTGCC AAACCATCCT TGCGGTTAAT 
GTTCCTTACA CAAGCTTGTT CAAGACCGTA ACTGTCCGAT GTGGTTGCTG TACCAATCTC 
CTTTCGGTGA ACATGAGATC ATATGTCCTC CCAGCTTCTA ACCAGCTCCA GCTCCAGCTC 
GGTCCTCACT CTTACTTCAA TCCCCAGGAT ATTCTGGAGG AGCTGAGAGA TGCACCGTCT 
AACATGAATA TGATGATGAT GAATCAACAT CCTACTATGA ATGACATACC ATCTTTCATG 
GATCTTCATC AACAACATGA GATTCCTAAA GCACCACCCG TTAACCGCCC TCCAGAGAAA 
AGACAGAGAG TCCCATCCGC ATATAACCGA TTCATCAAGG AGGAGATCCA ACGTATCAAA 
GCTGGTAATC CTGATATAAG CCACAGAGAA GCCTTTAGTG CTGCTGCCAA GAATTGGGCC 
CACTTCCCCC ACATACACTT CGGGCTCGTG CCAGACAATC AACCCGTGAA GAAAACCAAC 
ATGCCCCAAC AGGAGGGAGA GGATAACATG GTGATGAAAG AAGGGTTCTA CGCTCCTGCA 
GCTGCTAACG TTGGTGTGAC TCCTTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFO ( NM_001337117.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001323472.1 Arabidopsis thaliana Plant-specific transcription factor YABBY family protein (AFO), mRNA.

Price & Availability↑

CloneID	OAb36634
Clone ID Related Accession (Same CDS sequence)	NM_001337117.1
Accession Version	NM_001337117.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Plant-specific transcription factor YABBY family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071).

ATGAGATCAT ATGTCCTCCC AGCTTCTAAC CAGCTCCAGC TCCAGCTCGG TCCTCACTCT 
TACTTCAATC CCCAGGATAT TCTGGAGGAG CTGAGAGATG CACCGTCTAA CATGAATATG 
ATGATGATGA ATCAACATCC TACTATGAAT GACATACCAT CTTTCATGGA TCTTCATCAA 
CAACATGAGA TTCCTAAAGC ACCACCCGTT AACCGCCCTC CAGAGAAAAG ACAGAGAGTC 
CCATCCGCAT ATAACCGATT CATCAAGGAG GAGATCCAAC GTATCAAAGC TGGTAATCCT 
GATATAAGCC ACAGAGAAGC CTTTAGTGCT GCTGCCAAGA ATTGGGCCCA CTTCCCCCAC 
ATACACTTCG GGCTCGTGCC AGACAATCAA CCCGTGAAGA AAACCAACAT GCCCCAACAG 
GAGGGAGAGG ATAACATGGT GATGAAAGAA GGGTTCTACG CTCCTGCAGC TGCTAACGTT 
GGTGTGACTC CTTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001323472.1
CDS	190..687
Translation	MRSYVLPASNQLQLQLGPHSYFNPQDILEELRDAPSNMNMMMMNQHPTMNDIPSFMDLHQQHEIPKAPPVNRPPEKRQRVPSAYNRFIKEEIQRIKAGNPDISHREAFSAAAKNWAHFPHIHFGLVPDNQPVKKTNMPQQEGEDNMVMKEGFYAPAAANVGVTPY

Target ORF information:

RefSeq Version	NM_001337117.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Plant-specific transcription factor YABBY family protein (AFO), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001337117.1

ORF Insert Sequence:

ATGAGATCAT ATGTCCTCCC AGCTTCTAAC CAGCTCCAGC TCCAGCTCGG TCCTCACTCT 
TACTTCAATC CCCAGGATAT TCTGGAGGAG CTGAGAGATG CACCGTCTAA CATGAATATG 
ATGATGATGA ATCAACATCC TACTATGAAT GACATACCAT CTTTCATGGA TCTTCATCAA 
CAACATGAGA TTCCTAAAGC ACCACCCGTT AACCGCCCTC CAGAGAAAAG ACAGAGAGTC 
CCATCCGCAT ATAACCGATT CATCAAGGAG GAGATCCAAC GTATCAAAGC TGGTAATCCT 
GATATAAGCC ACAGAGAAGC CTTTAGTGCT GCTGCCAAGA ATTGGGCCCA CTTCCCCCAC 
ATACACTTCG GGCTCGTGCC AGACAATCAA CCCGTGAAGA AAACCAACAT GCCCCAACAG 
GAGGGAGAGG ATAACATGGT GATGAAAGAA GGGTTCTACG CTCCTGCAGC TGCTAACGTT 
GGTGTGACTC CTTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Nature402(6763)761-8(1999 Dec)
Lin X,Kaul S,Rounsley S,Shea TP,Benito MI,Town CD,Fujii CY,Mason T,Bowman CL,Barnstead M,Feldblyum TV,Buell CR,Ketchum KA,Lee J,Ronning CM,Koo HL,Moffat KS,Cronin LA,Shen M,Pai G,Van Aken S,Umayam L,Tallon LJ,Gill JE,Adams MD,Carrera AJ,Creasy TH,Goodman HM,Somerville CR,Copenhaver GP,Preuss D,Nierman WC,White O,Eisen JA,Salzberg SL,Fraser CM,Venter JC

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AFO cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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