CSI1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	CSI1
Entrez Gene ID	816747
Full Name	binding protein
Synonyms	POM-POM 2,POM2,T16B14.2,cellulose synthase-interactive protein 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_127781.5	NP_179803.4	binding protein

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	AT2G22125	NP_179803.4
Arabidopsis thaliana (thale cress)	AT1G44120	NP_175078.1
Oryza sativa (rice)	Os06g0223800	NP_001057185.2
Arabidopsis thaliana (thale cress)	AT1G77460	NP_177870.2

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Lin X, Kaul S, Rounsley S, Shea TP, Benito MI, Town CD, Fujii CY, Mason T, Bowman CL, Barnstead M, Feldblyum TV, Buell CR, Ketchum KA, Lee J, Ronning CM, Koo HL, Moffat KS, Cronin LA, Shen M, Pai G, Van Aken S, Umayam L, Tallon LJ, Gill JE, Adams MD, Carrera AJ, Creasy TH, Goodman HM, Somerville CR, Copenhaver GP, Preuss D, Nierman WC, White O, Eisen JA, Salzberg SL, Fraser CM, Venter JC
Nature402(6763)761-8(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the sustained cellulose synthesis is important for salt stress tolerance in Arabidopsis.
Title: Cellulose synthesis genes CESA6 and CSI1 are important for salt stress tolerance in Arabidopsis.

CSI1 and the COMPANIONS OF CELLULOSE SYNTHASE proteins fulfil distinct functions during cellulose synthesis.
Title: The cellulose synthase companion proteins act non-redundantly with CELLULOSE SYNTHASE INTERACTING1/POM2 and CELLULOSE SYNTHASE 6.

Small CESA compartments (SmaCCs) or microtubule-associated cellulose synthase compartments (MASCs) are critical for fast recovery of CSCs to the plasma membrane after stress is relieved in Arabidopsis thaliana. This SmaCC/MASC-mediated fast recovery of CSCs is dependent on CELLULOSE SYNTHASE INTERACTIVE1 (CSI1), a protein previously known to represent the link between CSCs and cortical microtubules.
Title: CELLULOSE SYNTHASE INTERACTIVE1 Is Required for Fast Recycling of Cellulose Synthase Complexes to the Plasma Membrane in Arabidopsis.

The Cellulose synthase complex (CSC)-associated proteins KORRIGAN1 (KOR1) and POM2/CELLULOSE SYNTHASE INTERACTIVE PROTEIN1 (CSI1) were differentially affected by CESTRIN treatment, indicating different forms of association with the plasma membrane CSCs.
Title: CESA TRAFFICKING INHIBITOR inhibits cellulose deposition and interferes with the trafficking of cellulose synthase complexes and their associated proteins KORRIGAN1 and POM2/CELLULOSE SYNTHASE INTERACTIVE PROTEIN1.

CSI1 directly mediates the interaction between cellulose synthase complexes and microtubules.
Title: Cellulose synthase interactive protein 1 (CSI1) mediates the intimate relationship between cellulose microfibrils and cortical microtubules.

The following CSI1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CSI1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb07653	NM_127781.5 Latest version!	Arabidopsis thaliana binding protein (CSI1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CSI1 ( NM_127781.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_179803.4 Arabidopsis thaliana binding protein (CSI1), mRNA.

Price & Availability↑

CloneID	OAb07653
Clone ID Related Accession (Same CDS sequence)	NM_127781.5
Accession Version	NM_127781.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	binding protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071). On Sep 12, 2016 this sequence version replaced NM_127781.4.

ATGACAAGTG CTCTTGGATG GAGATTTCCT TCCACTAATG GAAATGGCCT TGCCCCAAGT 
GACACGGAGA GAAATGGGGA TATGAAGATG CATGATTCAG AGCCCCCAAC TCCACATTCA 
ACCACAAAGA TGAGTTTGAG AGACCGGACA ACCAGCATGG AAGATCCAGA TGGGACATTA 
GCAAGTGTTG CCCAATGCAT TGAGCAGCTG CGCCAAGGTT CATCATCTGC ACAAGAAAGA 
GAATATTGTC TGAAGCAGTT ACTGGACCTT ATAGAAATGC GTGAGAATGC ATTCAGTGCT 
GTCGGATCAC ACTCCCAAGC TGTGCCAGTA CTTGTCTCTC TTCTCCGATC GGGATCAGTT 
GGAGTGAAGA TACAAGCTGC AACCGTCTTG GGTTCACTCT GCAAGGAGAA CGAACTTAGA 
GTTAAGGTGT TACTTGGGGG ATGCATTCCT CCATTGCTTG GTCTTCTCAA GTCCAGTTCA 
GTCGAAGGGC AGATCGCTGC AGCAAAGACA ATCTATGCTG TTTCTGAAGG TGGGGTTAAA 
GACCATGTTG GATCAAAAAT ATTTTCCACT GAGGGAGTTG TACCGGTTCT GTGGGACCAA 
TTGCGCAGTG GAAATAAAAA AGGAGAGGTT GATGGTTTGT TAACTGGTGC ACTTAAGAAC 
CTTTCTAGTA CCACAGAGGG ATTTTGGTCT GAGACAATTC GAGCTGGGGG AGTGGATGTA 
CTTGTGAAGC TGCTCACAAG CGGGCAGTCA AGTACACTAT CTAATGTCTG CTTCCTTCTT 
GCGTGTATGA TGATGGAAGA CGCCTCTGTT TGTTCCAGTG TATTGACTGC AGACATTACA 
AAACAGCTTC TCAAATTATT AGGATCGGGT AATGAAGCCC CAGTGAGGGC AGAGGCTGCT 
GCTGCTCTCA AGTCGCTTTC TGCTCAATCT AAGGAAGCAA AGCGGGAAAT CGCTAATTCT 
AATGGTATTC CTGTTTTAAT AAATGCAACA ATAGCTCCAT CTAAAGAATT CATGCAAGGT 
GAATATGCGC AAGCATTACA AGAAAATGCA ATGTGTGCCC TTGCTAATAT TTCCGGTGGT 
TTGTCATATG TCATATCAAG CCTTGGTCAG AGCCTTGAAT CTTGCTCTTC GCCTGCTCAG 
ACTGCCGACA CCCTTGGGGC TTTAGCTTCA GCATTGATGA TATATGATGG CAAGGCAGAA 
ACTACAAGAG CATCAGATCC ATTGGTCGTT GAACAGACTC TGCTGAAGCA GTTTAAGCCT 
CGTCTACCAT TCTTGGTGCA AGAACGTACA ATTGAAGCAC TTGCAAGTTT GTATGGGAAT 
TCCATACTCT CTGTCAAACT TTCCAACTCG GATGCAAAAC GTTTGCTTGT TGGTCTAATA 
ACGATGGCGG TGAATGAGGT TCAAGATGAG CTTGTAAAGG CTCTTCTGAT GCTGTGTAAC 
CATGAAGGTA GCCTGTGGCA AGCACTACAG GGCCGTGAAG GGATCCAATT GCTAATATCT 
CTTTTGGGTC TTTCATCCGA ACAGCAGCAA GAATGTGCTG TTGCACTTCT TTGCCTTTTG 
TCTAACGAAA ATGATGAAAG CAAGTGGGCC ATCACTGCTG CTGGAGGTAT ACCTCCACTT 
GTTCAAATTC TAGAGACTGG GTCGGCCAAA GCCAGGGAAG ACTCTGCAAC TATCCTTAGG 
AACTTGTGCA ATCATAGCGA AGATATACGT GCTTGTGTTG AAAGTGCGGA TGCTGTCCCT 
GCTCTCTTAT GGCTACTAAA GAATGGTAGT CCAAATGGTA AAGAAATTGC CGCCAAGACA 
TTGAATCATT TGATTCATAA ATCAGATACT GCAACTATCA GTCAGCTTAC TGCTTTGCTG 
ACCAGTGATC TACCTGAGTC TAAAATTTAC GTTTTGGATG CATTGAAGAG TATGCTTTCT 
GTGGTCCCAT TCAATGACAT GCTACGTGAA GGCAGTGCTT CCAATGATGC TATTGAGACA 
ATGATCAAGT TGATGAGCTC TGGTAAAGAA GAAACTCAAG CAAATTCAGC ATCAGCTCTT 
GCAGCTATCT TCCAGAGCAG AAAGGATTTG CGTGAGAGTG CTCTAGCCCT CAAAACTCTC 
TTGTCGGCCA TAAAACTGTT AAATGTGGAT TCGGAGAGGA TCCTTGTTGA GTCTTGCCGC 
TGCCTGGCTG CAATTTTACT TTCAATTAAG GAGAATCGCG ATGTGGCCAT TTCTGCCAGA 
GAAGCATTGC CTACCATAGT ATCTCTAGCC AACTCTTCTG TTCTAGAAGT TGCTGAGCAA 
GGAATGTGCG CTTTGGCTAA TCTTATATTG GACAGTGAAG TGTCAGAGAA GGTCATTGTC 
GAAGATATCA TCTTGTCCGC CACTAGAATC TTACGTGAAG GTACAGTGTC TGGGAAGACA 
CTTGCTGCTG CGGCAATTGC CCGCCTCCTT AGTCGTCGCC GAATTGATTC TGCATTGACA 
GATTCTGTGA ACCGAGCTGG AACAGTGCTT ACCTTAGTGT CTCTTCTAGA ATCTGCCGAT 
GGAAGATCTG ATGCAATATC AGAGGCCCTA GATGCGCTTG CTATATTCTC AAGGTCAGGA 
GCTAATGGAA ATGTAAAACC AGCTTGGGCT GTTTTGGCTG AATCTCCCAA CAGTATGGCC 
CCAATAGTCT CATCTATAGT GAGTGTTGCT AATCCTTCAC TACAGGACAA AGCTATTGAA 
GTTTTGTCAA GACTTTGCCG AGATCAGCCT ATTGTTTTGG GAAACATGGT CAACAATGCC 
AGAGATTGCG TGTCATCTAT AGCTAAAAGG GTGATAAATA CCAGGGACCC GAAAATAAAA 
ATTGGTGGAG CTGCTATTAT TATCTGTGCT GCCAAAGTTG ACGACCAGAA GATGATAGAA 
AATCTGAATG AGACACAATT GTGTGCCAAA TTCGTTCAAG CACTTGTGGG GATCCTAGAT 
TCTGTGCAAG ATCAGGAAAA GGATGAGAAA GATAAAATCT GCATATGCAT TCATCCCAAA 
GAAAAGGAAG AAGATGAAGA AGAAGAGGCA ACAGAAAATA GGGAGGGTTC CACGGGGGCA 
ACAGTTATTT CTGGAGACAA TTTAGCCATC TGGTTGCTCT CGGTTCTTTC TTGTCATGAT 
GAAAAGAGCA GAGCAGTAAT ATTGGAGTCA GAAGGCATTG AGTTGATTAC TGACAGAATA 
GGCAACCGTT TTCTCCAGGC TGATAATGGA GAAGATGCCA ACATTTGGGT TTGTGCTTTA 
TTGCTGGCCA TACTTTTCCA AGACAGAGAA ATCACTCGCG CACATGCAAC AATGAAGGCT 
GTTCCAGTGC TCTCAAATCT TGTGAAGTCA GAGGAATACG CAGACAGATA TTTTGCAGCA 
CAAGCTCTAG CTAGTCTTGT CTGTAACGGT AGTCGCGGAA CTCTTCTATC TGTTGCAAAC 
TCCGGTGCTG CGGCTGGGTT TATATCATTG CTTGGTTGTT CTGATGATGA CATAAAAGAA 
CTTCTGCAGC TGTCGCAGGA GTTCACTTTA GTGCGGTACC CAGACCAGGT TGCCCTTGAA 
AGGCTTTTCC GAGTTGAAGA TATTAGAGTT GGGGCTACTT CTCGAAAGGC GATTCCCTTA 
CTAGTTGAAC TTCTTAAACC AATTCCAGAC CGCCCCGGTG CTCCTCTACT TTCACTTAAT 
CTTCTAACTC AGCTTGCTGG GGACTGTCCA CAAAATATGA TTGTCATGGT AGAGTCAGGT 
GCTCTTGAGG GTCTCTCGAA ATATCTTTCC CTTGGACCTC AGGACGAGCA AGAGGAAGCT 
GCAACAGGTC TTTTAGGCAT CTTATTTAGC AGTGCTGAAA TTCGAAGGCA TGAGTCAGCA 
TTTGGCGCGG TCAGCCAACT TGTAGCTGTT TTGCGTCTAG GTGGAAGGGG GGCAAGGTAC 
AGTGCCGCCA AGGCATTGGA TAGTCTCTTT ACTGCTGACC ATATAAGAAA TGCAGAGTCT 
TCTAGACAAG CTGTTCAGCC ATTGGTAGAG ATTCTCAACA CTGGTTCAGA GAGGGAGCAG 
CATGCTGCTA TTGCTGCACT TGTTAGGTTG TTGAGTGATA ATCCATCACG GGCTCTGGCA 
GTTGCAGATG TGGAGATGAA TGCGGTGGAT GTTCTCTGCA GGATCCTTTC TTCAAACTAT 
ACGATGGAAC TGAAGGGAGA TGCTGCAGAG CTGTGTTATG TTCTGTTTGC AAATACAAGG 
ATTAGATCTA CAGTTGCTGC AGCGCGTTGT GTTGAGCCAC TTGTCTCTCT TCTTGTGACT 
GAGTTCAGCC CTGCTCAGCA TTCAGTTGTG CGTGCACTGG ACAAGCTTGT CGACGATGAA 
CAACTGGCTG AACTAGTTGC AGCTCATGGC GCTGTGGTAC CTCTTGTTGG CCTTCTCTAT 
GGTAAAAACT ATGTGCTTCA TGAGGCTATA TCCAGGGCTC TAGTAAAGTT GGGTAAGGAC 
AGGCCTGCTT GTAAACTGGA AATGGTGAAA GCTGGAGTTA TCGACTGCGT ACTTGATATT 
CTCCACGAAG CTCCAGATTT TCTATGTGCT GCGTTTTCAG AACTGCTTCG AATCCTCACA 
AATAATGCTA CTATTGCCAA GGGACAATCT GCTGCGAAAG TGGTGGAGCC ACTTTTCCAT 
TTGCTGACGA GGTTGGAGTT TGGAGCTGAT GGACAGCACA GTGCTCTGCA AGTTCTGGTA 
AATATCTTAG AACATCCACA ATGCCGCGCT GATTATACAC TTACACCGCA CCAAGTAATT 
GAGCCTTTAA TTCCGCTACT GGAATCTCCA TCCCCAGCAG TCCAGCAGTT GGCAGCTGAG 
CTTCTATCTC ATCTTCTCTA CGAGGAGCAT CTGCAGAAAG ATCCATTAAC GCAACTTGCG 
ATTGGTCCTC TAATCCATGT GCTGGGATCC GGCATACACC TATTACAGCA GAGAGCTGTG 
AAAGCTCTCG TCAGCATTGC TCTAACATGG CCAAATGAAA TAGCTAAAGA AGGTGGTGTG 
AGTGAGCTGT CAAAGGTGAT ACTGCAAGCT GATCCATCGC TCTCCAATGT GTTATGGGAG 
TCTGCAGCAT CAATTTTGGT TATCATCTTG CAATTTAGCT CCGAATTTTA CCTGGAAGTT 
CCTGTTGCTG TTCTTGTAAG ACTGCTTCGT TCTGCATCTG AAAACACCGT GGTTGGTGCA 
CTTAACGCTC TTCTAGTGTT GGAAAGCGAT GATGGAACCA GTGCAGAGTC CATGGCTGAA 
AGTGGTGCCA TAGAAGCTCT GCTTGATCTT TTAAGGTCTC ATCAGTGTGA GGACACAGCA 
GCGAGACTGC TCGAGGTACT GCTCAACAAC GTGAAGATTA GAGATTCAAA AGCCACCAAG 
ACTGCAATCT TGCCATTGTC TCAGTACCTC TTGGATCCAC AAACTCAAGC TCAGCAGGCG 
CGGTTACTAG CAACTTTGGC CCTTGGTGAT CTTTTCCAGA ATGAAGCTCT TGCTAGAAGC 
ACCGATGCAG CTTCTGCTTG CCGTGCTTTA GTCAATGTCC TTGAAGAACA ACCCACAGAA 
GAAATGAAAG TAGTTGCTAT ATGTGCTCTG CAAAATCTCG TCATGTATAG CCGATCTAAC 
AAAAGAGCAG TTGCCGAGGC AGGTGGTGTG CAGGTCGTCT TGGATCTGAT CAGTTCCAGT 
GATCCTGAAA CGTCTGTTCA GGCTGCAATG TTCGTAAAAC TACTCTTCTC TAATCACACC 
GTTCAAGAGT ACGCATCTAG CGAAACCGTC AGAGCAATAA CTGCTGCCAT TGAGAAAGAC 
TTATGGGCCA CTGGAACCGT GAATGATGAA TACCTAAAAG CCTTAAACTC TTTGTTTAAC 
AACTTCCCGC GTCTAAGAGC TACAGAGCCT GCAACACTAA GTATACCACA TCTAGTTACA 
TCCCTCAAGA CAGGATCAGA AGCAACACAA GAAGCTGCCT TGGATGCATT GTTTCTTCTC 
AGACAAGCTT GGTCAGCCTG TCCTGCCGAA GTTTCCAGGG CCCAATCAGT TGCTGCTGCA 
GACGCCATCC CCCTACTTCA GTACCTAATC CAATCAGGTC CACCACGGTT CCAGGAGAAA 
GCTGAATTCC TCTTGCAGTG TTTGCCAGGT ACTCTGGTAG TCACTATCAA ACGTGGTAAC 
AACATGAAGC AATCCGTTGG TAACCCGAGT GTGTTCTGCA AGATAACACT CGGCAACAAT 
CCTCCTAGAC AGACCAAGGT GATATCGACA GGTCCTAATC CAGAGTGGGA CGAGAGCTTC 
TCATGGTCAT TTGAGAGCCC TCCCAAAGGT CAAAAACTCC ACATTTCATG CAAGAACAAA 
AGCAAAATGG GAAAGAGTTC CTTTGGGAAA GTAACGATCC AAATCGATAG GGTGGTGATG 
CTGGGAGCAG TCGCAGGTGA ATATAGTTTG TTACCAGAAA GCAAAAGTGG TCCCAGAAAC 
CTTGAGATAG AATTCCAGTG GTCTAACAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_179803.4
CDS	398..6850
Translation	MTSALGWRFPSTNGNGLAPSDTERNGDMKMHDSEPPTPHSTTKMSLRDRTTSMEDPDGTLASVAQCIEQLRQGSSSAQEREYCLKQLLDLIEMRENAFSAVGSHSQAVPVLVSLLRSGSVGVKIQAATVLGSLCKENELRVKVLLGGCIPPLLGLLKSSSVEGQIAAAKTIYAVSEGGVKDHVGSKIFSTEGVVPVLWDQLRSGNKKGEVDGLLTGALKNLSSTTEGFWSETIRAGGVDVLVKLLTSGQSSTLSNVCFLLACMMMEDASVCSSVLTADITKQLLKLLGSGNEAPVRAEAAAALKSLSAQSKEAKREIANSNGIPVLINATIAPSKEFMQGEYAQALQENAMCALANISGGLSYVISSLGQSLESCSSPAQTADTLGALASALMIYDGKAETTRASDPLVVEQTLLKQFKPRLPFLVQERTIEALASLYGNSILSVKLSNSDAKRLLVGLITMAVNEVQDELVKALLMLCNHEGSLWQALQGREGIQLLISLLGLSSEQQQECAVALLCLLSNENDESKWAITAAGGIPPLVQILETGSAKAREDSATILRNLCNHSEDIRACVESADAVPALLWLLKNGSPNGKEIAAKTLNHLIHKSDTATISQLTALLTSDLPESKIYVLDALKSMLSVVPFNDMLREGSASNDAIETMIKLMSSGKEETQANSASALAAIFQSRKDLRESALALKTLLSAIKLLNVDSERILVESCRCLAAILLSIKENRDVAISAREALPTIVSLANSSVLEVAEQGMCALANLILDSEVSEKVIVEDIILSATRILREGTVSGKTLAAAAIARLLSRRRIDSALTDSVNRAGTVLTLVSLLESADGRSDAISEALDALAIFSRSGANGNVKPAWAVLAESPNSMAPIVSSIVSVANPSLQDKAIEVLSRLCRDQPIVLGNMVNNARDCVSSIAKRVINTRDPKIKIGGAAIIICAAKVDDQKMIENLNETQLCAKFVQALVGILDSVQDQEKDEKDKICICIHPKEKEEDEEEEATENREGSTGATVISGDNLAIWLLSVLSCHDEKSRAVILESEGIELITDRIGNRFLQADNGEDANIWVCALLLAILFQDREITRAHATMKAVPVLSNLVKSEEYADRYFAAQALASLVCNGSRGTLLSVANSGAAAGFISLLGCSDDDIKELLQLSQEFTLVRYPDQVALERLFRVEDIRVGATSRKAIPLLVELLKPIPDRPGAPLLSLNLLTQLAGDCPQNMIVMVESGALEGLSKYLSLGPQDEQEEAATGLLGILFSSAEIRRHESAFGAVSQLVAVLRLGGRGARYSAAKALDSLFTADHIRNAESSRQAVQPLVEILNTGSEREQHAAIAALVRLLSDNPSRALAVADVEMNAVDVLCRILSSNYTMELKGDAAELCYVLFANTRIRSTVAAARCVEPLVSLLVTEFSPAQHSVVRALDKLVDDEQLAELVAAHGAVVPLVGLLYGKNYVLHEAISRALVKLGKDRPACKLEMVKAGVIDCVLDILHEAPDFLCAAFSELLRILTNNATIAKGQSAAKVVEPLFHLLTRLEFGADGQHSALQVLVNILEHPQCRADYTLTPHQVIEPLIPLLESPSPAVQQLAAELLSHLLYEEHLQKDPLTQLAIGPLIHVLGSGIHLLQQRAVKALVSIALTWPNEIAKEGGVSELSKVILQADPSLSNVLWESAASILVIILQFSSEFYLEVPVAVLVRLLRSASENTVVGALNALLVLESDDGTSAESMAESGAIEALLDLLRSHQCEDTAARLLEVLLNNVKIRDSKATKTAILPLSQYLLDPQTQAQQARLLATLALGDLFQNEALARSTDAASACRALVNVLEEQPTEEMKVVAICALQNLVMYSRSNKRAVAEAGGVQVVLDLISSSDPETSVQAAMFVKLLFSNHTVQEYASSETVRAITAAIEKDLWATGTVNDEYLKALNSLFNNFPRLRATEPATLSIPHLVTSLKTGSEATQEAALDALFLLRQAWSACPAEVSRAQSVAAADAIPLLQYLIQSGPPRFQEKAEFLLQCLPGTLVVTIKRGNNMKQSVGNPSVFCKITLGNNPPRQTKVISTGPNPEWDESFSWSFESPPKGQKLHISCKNKSKMGKSSFGKVTIQIDRVVMLGAVAGEYSLLPESKSGPRNLEIEFQWSNK

Target ORF information:

RefSeq Version	NM_127781.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana binding protein (CSI1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_127781.5

ORF Insert Sequence:

ATGACAAGTG CTCTTGGATG GAGATTTCCT TCCACTAATG GAAATGGCCT TGCCCCAAGT 
GACACGGAGA GAAATGGGGA TATGAAGATG CATGATTCAG AGCCCCCAAC TCCACATTCA 
ACCACAAAGA TGAGTTTGAG AGACCGGACA ACCAGCATGG AAGATCCAGA TGGGACATTA 
GCAAGTGTTG CCCAATGCAT TGAGCAGCTG CGCCAAGGTT CATCATCTGC ACAAGAAAGA 
GAATATTGTC TGAAGCAGTT ACTGGACCTT ATAGAAATGC GTGAGAATGC ATTCAGTGCT 
GTCGGATCAC ACTCCCAAGC TGTGCCAGTA CTTGTCTCTC TTCTCCGATC GGGATCAGTT 
GGAGTGAAGA TACAAGCTGC AACCGTCTTG GGTTCACTCT GCAAGGAGAA CGAACTTAGA 
GTTAAGGTGT TACTTGGGGG ATGCATTCCT CCATTGCTTG GTCTTCTCAA GTCCAGTTCA 
GTCGAAGGGC AGATCGCTGC AGCAAAGACA ATCTATGCTG TTTCTGAAGG TGGGGTTAAA 
GACCATGTTG GATCAAAAAT ATTTTCCACT GAGGGAGTTG TACCGGTTCT GTGGGACCAA 
TTGCGCAGTG GAAATAAAAA AGGAGAGGTT GATGGTTTGT TAACTGGTGC ACTTAAGAAC 
CTTTCTAGTA CCACAGAGGG ATTTTGGTCT GAGACAATTC GAGCTGGGGG AGTGGATGTA 
CTTGTGAAGC TGCTCACAAG CGGGCAGTCA AGTACACTAT CTAATGTCTG CTTCCTTCTT 
GCGTGTATGA TGATGGAAGA CGCCTCTGTT TGTTCCAGTG TATTGACTGC AGACATTACA 
AAACAGCTTC TCAAATTATT AGGATCGGGT AATGAAGCCC CAGTGAGGGC AGAGGCTGCT 
GCTGCTCTCA AGTCGCTTTC TGCTCAATCT AAGGAAGCAA AGCGGGAAAT CGCTAATTCT 
AATGGTATTC CTGTTTTAAT AAATGCAACA ATAGCTCCAT CTAAAGAATT CATGCAAGGT 
GAATATGCGC AAGCATTACA AGAAAATGCA ATGTGTGCCC TTGCTAATAT TTCCGGTGGT 
TTGTCATATG TCATATCAAG CCTTGGTCAG AGCCTTGAAT CTTGCTCTTC GCCTGCTCAG 
ACTGCCGACA CCCTTGGGGC TTTAGCTTCA GCATTGATGA TATATGATGG CAAGGCAGAA 
ACTACAAGAG CATCAGATCC ATTGGTCGTT GAACAGACTC TGCTGAAGCA GTTTAAGCCT 
CGTCTACCAT TCTTGGTGCA AGAACGTACA ATTGAAGCAC TTGCAAGTTT GTATGGGAAT 
TCCATACTCT CTGTCAAACT TTCCAACTCG GATGCAAAAC GTTTGCTTGT TGGTCTAATA 
ACGATGGCGG TGAATGAGGT TCAAGATGAG CTTGTAAAGG CTCTTCTGAT GCTGTGTAAC 
CATGAAGGTA GCCTGTGGCA AGCACTACAG GGCCGTGAAG GGATCCAATT GCTAATATCT 
CTTTTGGGTC TTTCATCCGA ACAGCAGCAA GAATGTGCTG TTGCACTTCT TTGCCTTTTG 
TCTAACGAAA ATGATGAAAG CAAGTGGGCC ATCACTGCTG CTGGAGGTAT ACCTCCACTT 
GTTCAAATTC TAGAGACTGG GTCGGCCAAA GCCAGGGAAG ACTCTGCAAC TATCCTTAGG 
AACTTGTGCA ATCATAGCGA AGATATACGT GCTTGTGTTG AAAGTGCGGA TGCTGTCCCT 
GCTCTCTTAT GGCTACTAAA GAATGGTAGT CCAAATGGTA AAGAAATTGC CGCCAAGACA 
TTGAATCATT TGATTCATAA ATCAGATACT GCAACTATCA GTCAGCTTAC TGCTTTGCTG 
ACCAGTGATC TACCTGAGTC TAAAATTTAC GTTTTGGATG CATTGAAGAG TATGCTTTCT 
GTGGTCCCAT TCAATGACAT GCTACGTGAA GGCAGTGCTT CCAATGATGC TATTGAGACA 
ATGATCAAGT TGATGAGCTC TGGTAAAGAA GAAACTCAAG CAAATTCAGC ATCAGCTCTT 
GCAGCTATCT TCCAGAGCAG AAAGGATTTG CGTGAGAGTG CTCTAGCCCT CAAAACTCTC 
TTGTCGGCCA TAAAACTGTT AAATGTGGAT TCGGAGAGGA TCCTTGTTGA GTCTTGCCGC 
TGCCTGGCTG CAATTTTACT TTCAATTAAG GAGAATCGCG ATGTGGCCAT TTCTGCCAGA 
GAAGCATTGC CTACCATAGT ATCTCTAGCC AACTCTTCTG TTCTAGAAGT TGCTGAGCAA 
GGAATGTGCG CTTTGGCTAA TCTTATATTG GACAGTGAAG TGTCAGAGAA GGTCATTGTC 
GAAGATATCA TCTTGTCCGC CACTAGAATC TTACGTGAAG GTACAGTGTC TGGGAAGACA 
CTTGCTGCTG CGGCAATTGC CCGCCTCCTT AGTCGTCGCC GAATTGATTC TGCATTGACA 
GATTCTGTGA ACCGAGCTGG AACAGTGCTT ACCTTAGTGT CTCTTCTAGA ATCTGCCGAT 
GGAAGATCTG ATGCAATATC AGAGGCCCTA GATGCGCTTG CTATATTCTC AAGGTCAGGA 
GCTAATGGAA ATGTAAAACC AGCTTGGGCT GTTTTGGCTG AATCTCCCAA CAGTATGGCC 
CCAATAGTCT CATCTATAGT GAGTGTTGCT AATCCTTCAC TACAGGACAA AGCTATTGAA 
GTTTTGTCAA GACTTTGCCG AGATCAGCCT ATTGTTTTGG GAAACATGGT CAACAATGCC 
AGAGATTGCG TGTCATCTAT AGCTAAAAGG GTGATAAATA CCAGGGACCC GAAAATAAAA 
ATTGGTGGAG CTGCTATTAT TATCTGTGCT GCCAAAGTTG ACGACCAGAA GATGATAGAA 
AATCTGAATG AGACACAATT GTGTGCCAAA TTCGTTCAAG CACTTGTGGG GATCCTAGAT 
TCTGTGCAAG ATCAGGAAAA GGATGAGAAA GATAAAATCT GCATATGCAT TCATCCCAAA 
GAAAAGGAAG AAGATGAAGA AGAAGAGGCA ACAGAAAATA GGGAGGGTTC CACGGGGGCA 
ACAGTTATTT CTGGAGACAA TTTAGCCATC TGGTTGCTCT CGGTTCTTTC TTGTCATGAT 
GAAAAGAGCA GAGCAGTAAT ATTGGAGTCA GAAGGCATTG AGTTGATTAC TGACAGAATA 
GGCAACCGTT TTCTCCAGGC TGATAATGGA GAAGATGCCA ACATTTGGGT TTGTGCTTTA 
TTGCTGGCCA TACTTTTCCA AGACAGAGAA ATCACTCGCG CACATGCAAC AATGAAGGCT 
GTTCCAGTGC TCTCAAATCT TGTGAAGTCA GAGGAATACG CAGACAGATA TTTTGCAGCA 
CAAGCTCTAG CTAGTCTTGT CTGTAACGGT AGTCGCGGAA CTCTTCTATC TGTTGCAAAC 
TCCGGTGCTG CGGCTGGGTT TATATCATTG CTTGGTTGTT CTGATGATGA CATAAAAGAA 
CTTCTGCAGC TGTCGCAGGA GTTCACTTTA GTGCGGTACC CAGACCAGGT TGCCCTTGAA 
AGGCTTTTCC GAGTTGAAGA TATTAGAGTT GGGGCTACTT CTCGAAAGGC GATTCCCTTA 
CTAGTTGAAC TTCTTAAACC AATTCCAGAC CGCCCCGGTG CTCCTCTACT TTCACTTAAT 
CTTCTAACTC AGCTTGCTGG GGACTGTCCA CAAAATATGA TTGTCATGGT AGAGTCAGGT 
GCTCTTGAGG GTCTCTCGAA ATATCTTTCC CTTGGACCTC AGGACGAGCA AGAGGAAGCT 
GCAACAGGTC TTTTAGGCAT CTTATTTAGC AGTGCTGAAA TTCGAAGGCA TGAGTCAGCA 
TTTGGCGCGG TCAGCCAACT TGTAGCTGTT TTGCGTCTAG GTGGAAGGGG GGCAAGGTAC 
AGTGCCGCCA AGGCATTGGA TAGTCTCTTT ACTGCTGACC ATATAAGAAA TGCAGAGTCT 
TCTAGACAAG CTGTTCAGCC ATTGGTAGAG ATTCTCAACA CTGGTTCAGA GAGGGAGCAG 
CATGCTGCTA TTGCTGCACT TGTTAGGTTG TTGAGTGATA ATCCATCACG GGCTCTGGCA 
GTTGCAGATG TGGAGATGAA TGCGGTGGAT GTTCTCTGCA GGATCCTTTC TTCAAACTAT 
ACGATGGAAC TGAAGGGAGA TGCTGCAGAG CTGTGTTATG TTCTGTTTGC AAATACAAGG 
ATTAGATCTA CAGTTGCTGC AGCGCGTTGT GTTGAGCCAC TTGTCTCTCT TCTTGTGACT 
GAGTTCAGCC CTGCTCAGCA TTCAGTTGTG CGTGCACTGG ACAAGCTTGT CGACGATGAA 
CAACTGGCTG AACTAGTTGC AGCTCATGGC GCTGTGGTAC CTCTTGTTGG CCTTCTCTAT 
GGTAAAAACT ATGTGCTTCA TGAGGCTATA TCCAGGGCTC TAGTAAAGTT GGGTAAGGAC 
AGGCCTGCTT GTAAACTGGA AATGGTGAAA GCTGGAGTTA TCGACTGCGT ACTTGATATT 
CTCCACGAAG CTCCAGATTT TCTATGTGCT GCGTTTTCAG AACTGCTTCG AATCCTCACA 
AATAATGCTA CTATTGCCAA GGGACAATCT GCTGCGAAAG TGGTGGAGCC ACTTTTCCAT 
TTGCTGACGA GGTTGGAGTT TGGAGCTGAT GGACAGCACA GTGCTCTGCA AGTTCTGGTA 
AATATCTTAG AACATCCACA ATGCCGCGCT GATTATACAC TTACACCGCA CCAAGTAATT 
GAGCCTTTAA TTCCGCTACT GGAATCTCCA TCCCCAGCAG TCCAGCAGTT GGCAGCTGAG 
CTTCTATCTC ATCTTCTCTA CGAGGAGCAT CTGCAGAAAG ATCCATTAAC GCAACTTGCG 
ATTGGTCCTC TAATCCATGT GCTGGGATCC GGCATACACC TATTACAGCA GAGAGCTGTG 
AAAGCTCTCG TCAGCATTGC TCTAACATGG CCAAATGAAA TAGCTAAAGA AGGTGGTGTG 
AGTGAGCTGT CAAAGGTGAT ACTGCAAGCT GATCCATCGC TCTCCAATGT GTTATGGGAG 
TCTGCAGCAT CAATTTTGGT TATCATCTTG CAATTTAGCT CCGAATTTTA CCTGGAAGTT 
CCTGTTGCTG TTCTTGTAAG ACTGCTTCGT TCTGCATCTG AAAACACCGT GGTTGGTGCA 
CTTAACGCTC TTCTAGTGTT GGAAAGCGAT GATGGAACCA GTGCAGAGTC CATGGCTGAA 
AGTGGTGCCA TAGAAGCTCT GCTTGATCTT TTAAGGTCTC ATCAGTGTGA GGACACAGCA 
GCGAGACTGC TCGAGGTACT GCTCAACAAC GTGAAGATTA GAGATTCAAA AGCCACCAAG 
ACTGCAATCT TGCCATTGTC TCAGTACCTC TTGGATCCAC AAACTCAAGC TCAGCAGGCG 
CGGTTACTAG CAACTTTGGC CCTTGGTGAT CTTTTCCAGA ATGAAGCTCT TGCTAGAAGC 
ACCGATGCAG CTTCTGCTTG CCGTGCTTTA GTCAATGTCC TTGAAGAACA ACCCACAGAA 
GAAATGAAAG TAGTTGCTAT ATGTGCTCTG CAAAATCTCG TCATGTATAG CCGATCTAAC 
AAAAGAGCAG TTGCCGAGGC AGGTGGTGTG CAGGTCGTCT TGGATCTGAT CAGTTCCAGT 
GATCCTGAAA CGTCTGTTCA GGCTGCAATG TTCGTAAAAC TACTCTTCTC TAATCACACC 
GTTCAAGAGT ACGCATCTAG CGAAACCGTC AGAGCAATAA CTGCTGCCAT TGAGAAAGAC 
TTATGGGCCA CTGGAACCGT GAATGATGAA TACCTAAAAG CCTTAAACTC TTTGTTTAAC 
AACTTCCCGC GTCTAAGAGC TACAGAGCCT GCAACACTAA GTATACCACA TCTAGTTACA 
TCCCTCAAGA CAGGATCAGA AGCAACACAA GAAGCTGCCT TGGATGCATT GTTTCTTCTC 
AGACAAGCTT GGTCAGCCTG TCCTGCCGAA GTTTCCAGGG CCCAATCAGT TGCTGCTGCA 
GACGCCATCC CCCTACTTCA GTACCTAATC CAATCAGGTC CACCACGGTT CCAGGAGAAA 
GCTGAATTCC TCTTGCAGTG TTTGCCAGGT ACTCTGGTAG TCACTATCAA ACGTGGTAAC 
AACATGAAGC AATCCGTTGG TAACCCGAGT GTGTTCTGCA AGATAACACT CGGCAACAAT 
CCTCCTAGAC AGACCAAGGT GATATCGACA GGTCCTAATC CAGAGTGGGA CGAGAGCTTC 
TCATGGTCAT TTGAGAGCCC TCCCAAAGGT CAAAAACTCC ACATTTCATG CAAGAACAAA 
AGCAAAATGG GAAAGAGTTC CTTTGGGAAA GTAACGATCC AAATCGATAG GGTGGTGATG 
CTGGGAGCAG TCGCAGGTGA ATATAGTTTG TTACCAGAAA GCAAAAGTGG TCCCAGAAAC 
CTTGAGATAG AATTCCAGTG GTCTAACAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Nature402(6763)761-8(1999 Dec)
Lin X,Kaul S,Rounsley S,Shea TP,Benito MI,Town CD,Fujii CY,Mason T,Bowman CL,Barnstead M,Feldblyum TV,Buell CR,Ketchum KA,Lee J,Ronning CM,Koo HL,Moffat KS,Cronin LA,Shen M,Pai G,Van Aken S,Umayam L,Tallon LJ,Gill JE,Adams MD,Carrera AJ,Creasy TH,Goodman HM,Somerville CR,Copenhaver GP,Preuss D,Nierman WC,White O,Eisen JA,Salzberg SL,Fraser CM,Venter JC

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CSI1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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