NEMF cDNA ORF clone, Aquila chrysaetos canadensis

Gene
Clones
gRNAs

Summary

Gene Symbol	NEMF
Entrez Gene ID	105407891
Full Name	nuclear export mediator factor
Gene Type	protein-coding
Organism	Aquila chrysaetos canadensis

mRNA and Protein(s)

mRNA	Protein	Name
XM_011587595.1	XP_011585897.1	nuclear export mediator factor NEMF isoform X2
XM_011587594.1	XP_011585896.1	nuclear export mediator factor NEMF isoform X1

Pathways from BioSystems
Gene Ontology

The following NEMF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEMF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAq24006	XM_011587594.1 Latest version!	Aquila chrysaetos canadensis nuclear export mediator factor (NEMF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAq24007	XM_011587595.1 Latest version!	Aquila chrysaetos canadensis nuclear export mediator factor (NEMF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEMF ( XM_011587594.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011585896.1 Aquila chrysaetos canadensis nuclear export mediator factor (NEMF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAq24006
Clone ID Related Accession (Same CDS sequence)	XM_011587594.1
Accession Version	XM_011587594.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	nuclear export mediator factor NEMF isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950931.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCCC GCTTCAGCAC CGTCGACATC CGCGCGGTGC TCGCCGAGCT CCGGCAGAGC 
TTATTGGGAA TGAGAGTAAA CAATGTTTAT GATGTGGATA ATAAGACGTA TCTTATTCGC 
CTTCAAAAAC CAGACTGCAA GGCCACGCTG CTGCTTGAAT CTGGTATACG CATTCACACG 
ACAGAGTTCG AGTGGCCAAA AAACATGATG CCATCTGGCT TTGCAATGAA GTGCCGTAAG 
CATTTAAAGA CCAGAAGACT TGTCAGTGTA AAACAGCTGG GTATAGACAG AATTGTGGAC 
TTCCAGTTTG GAAGCGATGA AGCTGCTTAT CACCTGATTA TTGAGCTGTA CGACAGGGGT 
AACATTGTCC TGACAGACCA TGAATACGTC ATTTTAAACA TTCTGAGGTT TCGCACGAAT 
GAAGCGGATG ATGTCAGATT TGCGGTTCGG GAACGATACC CAGTCGACAG TGCAAAAGTG 
CCTGCACCTC TGCCAACCTT GGAAAGGTTA ACTGAAATGA TATCAAATGC ACCCAAAGGG 
GAACAACTGA AGAGGGCTCT TAACCCGCAT CTTCCTTATG GAGCCACTCT CATCGAGCAT 
TGCCTTATAG AAGCTGGATT TTCTGGGTAC GTCAAAATAG ATCAGCATAT GGAAAGTAAA 
GAAAATATTG AAAAAGTGCT TGCTGCTTTA GAGAAAGCAG AAGAATATAT GACACTAACT 
GACAACTTCA GTGGAAAGGG TTACATTATC CAGAAAAAGG AGAAAAAGCC AAGTCTGGAA 
CCAGATAAAC CAGCAGAAGA TATCTACACA TATGAGGAAT TTCATCCTTT CTTGTTTTCT 
CAACATTCAA AATGTCCGTA CTTGGAATTT GACTCATTCA ATAAGGCAGC AGATGAGTTC 
TACTCCAAGC TAGAGGGACA GAAAATTGAT CTGAAAGCAT TGCAGCAGGA AAAACAAGCA 
TTGAAGAAAC TTGAAAATGT CCGTAGGGAT CATGAGCACA GACTAGAAGC TCTTCAGCAA 
GCTCAGGAAG CTGATAAGAT GAAAGGAGAA CTTATAGAAA TGAACTTGGA GATTGTTGAC 
CGAGCCATCC AGGTGGTTCG TAGCGCGTTA GCCAACCAGA TAGACTGGAC AGAGATTGGA 
GCGATTGTTA AAGAAGCTCA AGCCCAGGGA GACCCCGTTG CAAGTGCAAT CAAAGAATTA 
AAGCTTCAGA CAAATCATAT CACAATGCTG CTGAGGAACC CATATGTGTT ATCTGAAGAG 
GAAGAGGAGG AGGAGGATGC TAATTTAGAG AAAGAAGAAA CGGAAGAACC AAAGGGAAAA 
AAGAAAAAGA ATAAAAACAA ACAGTTGAAG AAACCTCAGA AAAACAAACC GTCATTAGTT 
GATGTTGATC TCAGTCTGTC TGCATATGCC AATGCCAAAA AGTACTATGA TCACAAAAGA 
CATGCAGCTA AGAAAACTCA GAAGACAGTA GAAGCTGCAG AAAAGGCATT CAAATCAGCC 
GAGAAGAAGA CGAAGCAGAC ACTGAGGGAA GTTCAGACAG TTACCACCAT TCAGAAAGCG 
AGAAAGGTCT ATTGGTTTGA GAAGTTCCTG TGGTTCATTA GTTCTGAGAA TTACCTTATT 
ATTGCTGGAA GAGATCAACA GCAGAATGAG CTGATTGTAA AGCGGTATCT TAAACCGGGG 
GACATATACG TGCATGCCGA TCTCCACGGA GCCACAAGCT GTGTGATCAA GAACCCGTCA 
GGTGAACCAA TCCCTCCACG AACTCTGACA GAGGCAGGCA CGATGGCCTT GTGTTACAGC 
GCTGCCTGGG AAGCCCGTGT TGTCACCAGT GCTTGGTGGG TCTCCCACAA TCAGGTTTCT 
AAAACTGCAC CTACAGGAGA ATACCTCACT ACTGGAAGCT TCATGATCCG AGGGAAGAAG 
AATTTTCTTC CACCTTCCTA TCTGATGATG GGCTTTAGCT TCTTGTTTAA GGTGGATGAA 
AGTTGTGTTT GGAGACACCG AGAAGAAAGG AAGATCAAAG TACAGGATGA GGATCTAGAA 
ATGGTTTCCA GCAGTGCCTG CGAACTGGTG TCTGAAGAAG TGGAGCTATT AGAAGGAGGA 
GATAGCAGCA ACGAAGAGGA AAAAGCAGAG TGTCAGGAAG CACCAGAGGA TGTGGAAGCC 
GTGTCTGAGA GCAATCGTGA TGAGGGCGTT GCTGACCTTG ACCAGGGAAG AGTAAACACA 
CCTCCTGCAC CAGAGGGTGA CTCCGAAGAG GATGATGGAG AGTCTGAAGA TGAAGTGGAA 
CATCTGGAGT CAAAGAGTGA AGTGAAGGAG GAAGAGGTAA ATTACCCAGA TACAACAATC 
GACCTGTCGC ATCTTCAGTC TCAAAGATCC CTGCAGAAAA TCGTCCCCAA AGAGGAAGAA 
CCCAACTTGA GTGACAGCAA GCCCCAGGGG CGAAGGCACC TGTCTGCCAA GGAGAGGAGA 
GAAATGAAGA AAAAGAAGCA GCAAAACGAC TCTGAGAACT TGGAGCCGCC TGAAGAGAAG 
GAGAAAGAGA CGGAAACACA GCCTCCCCCT GCTCCCAACA CCAATAAGGG TGTGCCGGCC 
CCTCAGCCCA TCAAGCGGGG CCAGAAGAGT AAAATGAAGA AGATGAAGGA GAAGTACAAG 
GACCAGGATG AGGAGGACCG GGAGCTCATC ATGAAGCTGC TGGGGTCTGC AGGGTCAAAC 
AAGGAGGAGA AAGGGAAAAA AGGGAAAAAG GGGAAGACAA AAGAAGAGCC AGCAAAGAAG 
CAACAGCAGA AACCCAAGGC TGCTCATTGT GGTGCTGGAA GGGGCAAGGA GATGCTCCCA 
GCAGGAGTCC TGCTGCACGA GTTGCAGGAC CCAGTCCTGC AGGAGCAGCA GGATGAGAAG 
GAGGAGCAAG ACCAGGATCA GCCGGGGGTT GAGGAAGGCG AGGCGTTGCT GGACTCCCTG 
ACGGGCCAGC CACACCCTGA GGATATTCTG CTCTTTGCTG TTCCCATCTG TGCTCCCTAC 
ACGGCGATGA TGAACTATAA GTATAAAGTC AAGCTCACTC CAGGCACCCA AAAGAAGGGA 
AAAGCTGCAA AGATTGCCTT GCATAATTTT ATGCAATCCA AAGAAGCTAG TGCAAGAGAA 
AAAGACCTGT TCCGCAGTGT AAAGGATACC GATTTGTCAA GAAATATTCC TGGTAAAGTG 
AAAGTGTCTG CACCTCATCT CCTGAACAGG AAAAAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011585896.1
CDS	39..3260
Translation	MKSRFSTVDIRAVLAELRQSLLGMRVNNVYDVDNKTYLIRLQKPDCKATLLLESGIRIHTTEFEWPKNMMPSGFAMKCRKHLKTRRLVSVKQLGIDRIVDFQFGSDEAAYHLIIELYDRGNIVLTDHEYVILNILRFRTNEADDVRFAVRERYPVDSAKVPAPLPTLERLTEMISNAPKGEQLKRALNPHLPYGATLIEHCLIEAGFSGYVKIDQHMESKENIEKVLAALEKAEEYMTLTDNFSGKGYIIQKKEKKPSLEPDKPAEDIYTYEEFHPFLFSQHSKCPYLEFDSFNKAADEFYSKLEGQKIDLKALQQEKQALKKLENVRRDHEHRLEALQQAQEADKMKGELIEMNLEIVDRAIQVVRSALANQIDWTEIGAIVKEAQAQGDPVASAIKELKLQTNHITMLLRNPYVLSEEEEEEEDANLEKEETEEPKGKKKKNKNKQLKKPQKNKPSLVDVDLSLSAYANAKKYYDHKRHAAKKTQKTVEAAEKAFKSAEKKTKQTLREVQTVTTIQKARKVYWFEKFLWFISSENYLIIAGRDQQQNELIVKRYLKPGDIYVHADLHGATSCVIKNPSGEPIPPRTLTEAGTMALCYSAAWEARVVTSAWWVSHNQVSKTAPTGEYLTTGSFMIRGKKNFLPPSYLMMGFSFLFKVDESCVWRHREERKIKVQDEDLEMVSSSACELVSEEVELLEGGDSSNEEEKAECQEAPEDVEAVSESNRDEGVADLDQGRVNTPPAPEGDSEEDDGESEDEVEHLESKSEVKEEEVNYPDTTIDLSHLQSQRSLQKIVPKEEEPNLSDSKPQGRRHLSAKERREMKKKKQQNDSENLEPPEEKEKETETQPPPAPNTNKGVPAPQPIKRGQKSKMKKMKEKYKDQDEEDRELIMKLLGSAGSNKEEKGKKGKKGKTKEEPAKKQQQKPKAAHCGAGRGKEMLPAGVLLHELQDPVLQEQQDEKEEQDQDQPGVEEGEALLDSLTGQPHPEDILLFAVPICAPYTAMMNYKYKVKLTPGTQKKGKAAKIALHNFMQSKEASAREKDLFRSVKDTDLSRNIPGKVKVSAPHLLNRKKK

Target ORF information:

RefSeq Version	XM_011587594.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis nuclear export mediator factor (NEMF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011587594.1

ORF Insert Sequence:

ATGAAGTCCC GCTTCAGCAC CGTCGACATC CGCGCGGTGC TCGCCGAGCT CCGGCAGAGC 
TTATTGGGAA TGAGAGTAAA CAATGTTTAT GATGTGGATA ATAAGACGTA TCTTATTCGC 
CTTCAAAAAC CAGACTGCAA GGCCACGCTG CTGCTTGAAT CTGGTATACG CATTCACACG 
ACAGAGTTCG AGTGGCCAAA AAACATGATG CCATCTGGCT TTGCAATGAA GTGCCGTAAG 
CATTTAAAGA CCAGAAGACT TGTCAGTGTA AAACAGCTGG GTATAGACAG AATTGTGGAC 
TTCCAGTTTG GAAGCGATGA AGCTGCTTAT CACCTGATTA TTGAGCTGTA CGACAGGGGT 
AACATTGTCC TGACAGACCA TGAATACGTC ATTTTAAACA TTCTGAGGTT TCGCACGAAT 
GAAGCGGATG ATGTCAGATT TGCGGTTCGG GAACGATACC CAGTCGACAG TGCAAAAGTG 
CCTGCACCTC TGCCAACCTT GGAAAGGTTA ACTGAAATGA TATCAAATGC ACCCAAAGGG 
GAACAACTGA AGAGGGCTCT TAACCCGCAT CTTCCTTATG GAGCCACTCT CATCGAGCAT 
TGCCTTATAG AAGCTGGATT TTCTGGGTAC GTCAAAATAG ATCAGCATAT GGAAAGTAAA 
GAAAATATTG AAAAAGTGCT TGCTGCTTTA GAGAAAGCAG AAGAATATAT GACACTAACT 
GACAACTTCA GTGGAAAGGG TTACATTATC CAGAAAAAGG AGAAAAAGCC AAGTCTGGAA 
CCAGATAAAC CAGCAGAAGA TATCTACACA TATGAGGAAT TTCATCCTTT CTTGTTTTCT 
CAACATTCAA AATGTCCGTA CTTGGAATTT GACTCATTCA ATAAGGCAGC AGATGAGTTC 
TACTCCAAGC TAGAGGGACA GAAAATTGAT CTGAAAGCAT TGCAGCAGGA AAAACAAGCA 
TTGAAGAAAC TTGAAAATGT CCGTAGGGAT CATGAGCACA GACTAGAAGC TCTTCAGCAA 
GCTCAGGAAG CTGATAAGAT GAAAGGAGAA CTTATAGAAA TGAACTTGGA GATTGTTGAC 
CGAGCCATCC AGGTGGTTCG TAGCGCGTTA GCCAACCAGA TAGACTGGAC AGAGATTGGA 
GCGATTGTTA AAGAAGCTCA AGCCCAGGGA GACCCCGTTG CAAGTGCAAT CAAAGAATTA 
AAGCTTCAGA CAAATCATAT CACAATGCTG CTGAGGAACC CATATGTGTT ATCTGAAGAG 
GAAGAGGAGG AGGAGGATGC TAATTTAGAG AAAGAAGAAA CGGAAGAACC AAAGGGAAAA 
AAGAAAAAGA ATAAAAACAA ACAGTTGAAG AAACCTCAGA AAAACAAACC GTCATTAGTT 
GATGTTGATC TCAGTCTGTC TGCATATGCC AATGCCAAAA AGTACTATGA TCACAAAAGA 
CATGCAGCTA AGAAAACTCA GAAGACAGTA GAAGCTGCAG AAAAGGCATT CAAATCAGCC 
GAGAAGAAGA CGAAGCAGAC ACTGAGGGAA GTTCAGACAG TTACCACCAT TCAGAAAGCG 
AGAAAGGTCT ATTGGTTTGA GAAGTTCCTG TGGTTCATTA GTTCTGAGAA TTACCTTATT 
ATTGCTGGAA GAGATCAACA GCAGAATGAG CTGATTGTAA AGCGGTATCT TAAACCGGGG 
GACATATACG TGCATGCCGA TCTCCACGGA GCCACAAGCT GTGTGATCAA GAACCCGTCA 
GGTGAACCAA TCCCTCCACG AACTCTGACA GAGGCAGGCA CGATGGCCTT GTGTTACAGC 
GCTGCCTGGG AAGCCCGTGT TGTCACCAGT GCTTGGTGGG TCTCCCACAA TCAGGTTTCT 
AAAACTGCAC CTACAGGAGA ATACCTCACT ACTGGAAGCT TCATGATCCG AGGGAAGAAG 
AATTTTCTTC CACCTTCCTA TCTGATGATG GGCTTTAGCT TCTTGTTTAA GGTGGATGAA 
AGTTGTGTTT GGAGACACCG AGAAGAAAGG AAGATCAAAG TACAGGATGA GGATCTAGAA 
ATGGTTTCCA GCAGTGCCTG CGAACTGGTG TCTGAAGAAG TGGAGCTATT AGAAGGAGGA 
GATAGCAGCA ACGAAGAGGA AAAAGCAGAG TGTCAGGAAG CACCAGAGGA TGTGGAAGCC 
GTGTCTGAGA GCAATCGTGA TGAGGGCGTT GCTGACCTTG ACCAGGGAAG AGTAAACACA 
CCTCCTGCAC CAGAGGGTGA CTCCGAAGAG GATGATGGAG AGTCTGAAGA TGAAGTGGAA 
CATCTGGAGT CAAAGAGTGA AGTGAAGGAG GAAGAGGTAA ATTACCCAGA TACAACAATC 
GACCTGTCGC ATCTTCAGTC TCAAAGATCC CTGCAGAAAA TCGTCCCCAA AGAGGAAGAA 
CCCAACTTGA GTGACAGCAA GCCCCAGGGG CGAAGGCACC TGTCTGCCAA GGAGAGGAGA 
GAAATGAAGA AAAAGAAGCA GCAAAACGAC TCTGAGAACT TGGAGCCGCC TGAAGAGAAG 
GAGAAAGAGA CGGAAACACA GCCTCCCCCT GCTCCCAACA CCAATAAGGG TGTGCCGGCC 
CCTCAGCCCA TCAAGCGGGG CCAGAAGAGT AAAATGAAGA AGATGAAGGA GAAGTACAAG 
GACCAGGATG AGGAGGACCG GGAGCTCATC ATGAAGCTGC TGGGGTCTGC AGGGTCAAAC 
AAGGAGGAGA AAGGGAAAAA AGGGAAAAAG GGGAAGACAA AAGAAGAGCC AGCAAAGAAG 
CAACAGCAGA AACCCAAGGC TGCTCATTGT GGTGCTGGAA GGGGCAAGGA GATGCTCCCA 
GCAGGAGTCC TGCTGCACGA GTTGCAGGAC CCAGTCCTGC AGGAGCAGCA GGATGAGAAG 
GAGGAGCAAG ACCAGGATCA GCCGGGGGTT GAGGAAGGCG AGGCGTTGCT GGACTCCCTG 
ACGGGCCAGC CACACCCTGA GGATATTCTG CTCTTTGCTG TTCCCATCTG TGCTCCCTAC 
ACGGCGATGA TGAACTATAA GTATAAAGTC AAGCTCACTC CAGGCACCCA AAAGAAGGGA 
AAAGCTGCAA AGATTGCCTT GCATAATTTT ATGCAATCCA AAGAAGCTAG TGCAAGAGAA 
AAAGACCTGT TCCGCAGTGT AAAGGATACC GATTTGTCAA GAAATATTCC TGGTAAAGTG 
AAAGTGTCTG CACCTCATCT CCTGAACAGG AAAAAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEMF ( XM_011587595.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011585897.1 Aquila chrysaetos canadensis nuclear export mediator factor (NEMF), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAq24007
Clone ID Related Accession (Same CDS sequence)	XM_011587595.1
Accession Version	XM_011587595.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	nuclear export mediator factor NEMF isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950931.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCCC GCTTCAGCAC CGTCGACATC CGCGCGGTGC TCGCCGAGCT CCGGCAGAGC 
TTATTGGGAA TGAGAGTAAA CAATGTTTAT GATGTGGATA ATAAGACGTA TCTTATTCGC 
CTTCAAAAAC CAGACTGCAA GGCCACGCTG CTGCTTGAAT CTGGTATACG CATTCACACG 
ACAGAGTTCG AGTGGCCAAA AAACATGATG CCATCTGGCT TTGCAATGAA GTGCCGTAAG 
CATTTAAAGA CCAGAAGACT TGTCAGTGTA AAACAGCTGG GTATAGACAG AATTGTGGAC 
TTCCAGTTTG GAAGCGATGA AGCTGCTTAT CACCTGATTA TTGAGCTGTA CGACAGGGGT 
AACATTGTCC TGACAGACCA TGAATACGTC ATTTTAAACA TTCTGAGGTT TCGCACGAAT 
GAAGCGGATG ATGTCAGATT TGCGGTTCGG GAACGATACC CAGTCGACAG TGCAAAAGTG 
CCTGCACCTC TGCCAACCTT GGAAAGGTTA ACTGAAATGA TATCAAATGC ACCCAAAGGG 
GAACAACTGA AGAGGGCTCT TAACCCGCAT CTTCCTTATG GAGCCACTCT CATCGAGCAT 
TGCCTTATAG AAGCTGGATT TTCTGGGTAC GTCAAAATAG ATCAGCATAT GGAAAGTAAA 
GAAAATATTG AAAAAGTGCT TGCTGCTTTA GAGAAAGCAG AAGAATATAT GACACTAACT 
GACAACTTCA GTGGAAAGGG TTACATTATC CAGAAAAAGG AGAAAAAGCC AAGTCTGGAA 
CCAGATAAAC CAGCAGAAGA TATCTACACA TATGAGGAAT TTCATCCTTT CTTGTTTTCT 
CAACATTCAA AATGTCCGTA CTTGGAATTT GACTCATTCA ATAAGGCAGC AGATGAGTTC 
TACTCCAAGC TAGAGGGACA GAAAATTGAT CTGAAAGCAT TGCAGCAGGA AAAACAAGCA 
TTGAAGAAAC TTGAAAATGT CCGTAGGGAT CATGAGCACA GACTAGAAGC TCTTCAGCAA 
GCTCAGGAAG CTGATAAGAT GAAAGGAGAA CTTATAGAAA TGAACTTGGA GATTGTTGAC 
CGAGCCATCC AGGTGGTTCG TAGCGCGTTA GCCAACCAGA TAGACTGGAC AGAGATTGGA 
GCGATTGTTA AAGAAGCTCA AGCCCAGGGA GACCCCGTTG CAAGTGCAAT CAAAGAATTA 
AAGCTTCAGA CAAATCATAT CACAATGCTG CTGAGGAACC CATATGTGTT ATCTGAAGAG 
GAAGAGGAGG AGGAGGATGC TAATTTAGAG AAAGAAGAAA CGGAAGAACC AAAGGGAAAA 
AAGAAAAAGA ATAAAAACAA ACAGTTGAAG AAACCTCAGA AAAACAAACC GTCATTAGTT 
GATGTTGATC TCAGTCTGTC TGCATATGCC AATGCCAAAA AGTACTATGA TCACAAAAGA 
CATGCAGCTA AGAAAACTCA GAAGACAGTA GAAGCTGCAG AAAAGGCATT CAAATCAGCC 
GAGAAGAAGA CGAAGCAGAC ACTGAGGGAA GTTCAGACAG TTACCACCAT TCAGAAAGCG 
AGAAAGGTCT ATTGGTTTGA GAAGTTCCTG TGGTTCATTA GTTCTGAGAA TTACCTTATT 
ATTGCTGGAA GAGATCAACA GCAGAATGAG CTGATTGTAA AGCGGTATCT TAAACCGGGG 
GACATATACG TGCATGCCGA TCTCCACGGA GCCACAAGCT GTGTGATCAA GAACCCGTCA 
GGTGAACCAA TCCCTCCACG AACTCTGACA GAGGCAGGCA CGATGGCCTT GTGTTACAGC 
GCTGCCTGGG AAGCCCGTGT TGTCACCAGT GCTTGGTGGG TCTCCCACAA TCAGGTTTCT 
AAAACTGCAC CTACAGGAGA ATACCTCACT ACTGGAAGCT TCATGATCCG AGGGAAGAAG 
AATTTTCTTC CACCTTCCTA TCTGATGATG GGCTTTAGCT TCTTGTTTAA GGTGGATGAA 
AGTTGTGTTT GGAGACACCG AGAAGAAAGG AAGATCAAAG TACAGGATGA GGATCTAGAA 
ATGGTTTCCA GCAGTGCCTG CGAACTGGTG TCTGAAGAAG TGGAGCTATT AGGAGGAGAT 
AGCAGCAACG AAGAGGAAAA AGCAGAGTGT CAGGAAGCAC CAGAGGATGT GGAAGCCGTG 
TCTGAGAGCA ATCGTGATGA GGGCGTTGCT GACCTTGACC AGGGAAGAGT AAACACACCT 
CCTGCACCAG AGGGTGACTC CGAAGAGGAT GATGGAGAGT CTGAAGATGA AGTGGAACAT 
CTGGAGTCAA AGAGTGAAGT GAAGGAGGAA GAGGTAAATT ACCCAGATAC AACAATCGAC 
CTGTCGCATC TTCAGTCTCA AAGATCCCTG CAGAAAATCG TCCCCAAAGA GGAAGAACCC 
AACTTGAGTG ACAGCAAGCC CCAGGGGCGA AGGCACCTGT CTGCCAAGGA GAGGAGAGAA 
ATGAAGAAAA AGAAGCAGCA AAACGACTCT GAGAACTTGG AGCCGCCTGA AGAGAAGGAG 
AAAGAGACGG AAACACAGCC TCCCCCTGCT CCCAACACCA ATAAGGGTGT GCCGGCCCCT 
CAGCCCATCA AGCGGGGCCA GAAGAGTAAA ATGAAGAAGA TGAAGGAGAA GTACAAGGAC 
CAGGATGAGG AGGACCGGGA GCTCATCATG AAGCTGCTGG GGTCTGCAGG GTCAAACAAG 
GAGGAGAAAG GGAAAAAAGG GAAAAAGGGG AAGACAAAAG AAGAGCCAGC AAAGAAGCAA 
CAGCAGAAAC CCAAGGCTGC TCATTGTGGT GCTGGAAGGG GCAAGGAGAT GCTCCCAGCA 
GGAGTCCTGC TGCACGAGTT GCAGGACCCA GTCCTGCAGG AGCAGCAGGA TGAGAAGGAG 
GAGCAAGACC AGGATCAGCC GGGGGTTGAG GAAGGCGAGG CGTTGCTGGA CTCCCTGACG 
GGCCAGCCAC ACCCTGAGGA TATTCTGCTC TTTGCTGTTC CCATCTGTGC TCCCTACACG 
GCGATGATGA ACTATAAGTA TAAAGTCAAG CTCACTCCAG GCACCCAAAA GAAGGGAAAA 
GCTGCAAAGA TTGCCTTGCA TAATTTTATG CAATCCAAAG AAGCTAGTGC AAGAGAAAAA 
GACCTGTTCC GCAGTGTAAA GGATACCGAT TTGTCAAGAA ATATTCCTGG TAAAGTGAAA 
GTGTCTGCAC CTCATCTCCT GAACAGGAAA AAGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011585897.1
CDS	39..3257
Translation	MKSRFSTVDIRAVLAELRQSLLGMRVNNVYDVDNKTYLIRLQKPDCKATLLLESGIRIHTTEFEWPKNMMPSGFAMKCRKHLKTRRLVSVKQLGIDRIVDFQFGSDEAAYHLIIELYDRGNIVLTDHEYVILNILRFRTNEADDVRFAVRERYPVDSAKVPAPLPTLERLTEMISNAPKGEQLKRALNPHLPYGATLIEHCLIEAGFSGYVKIDQHMESKENIEKVLAALEKAEEYMTLTDNFSGKGYIIQKKEKKPSLEPDKPAEDIYTYEEFHPFLFSQHSKCPYLEFDSFNKAADEFYSKLEGQKIDLKALQQEKQALKKLENVRRDHEHRLEALQQAQEADKMKGELIEMNLEIVDRAIQVVRSALANQIDWTEIGAIVKEAQAQGDPVASAIKELKLQTNHITMLLRNPYVLSEEEEEEEDANLEKEETEEPKGKKKKNKNKQLKKPQKNKPSLVDVDLSLSAYANAKKYYDHKRHAAKKTQKTVEAAEKAFKSAEKKTKQTLREVQTVTTIQKARKVYWFEKFLWFISSENYLIIAGRDQQQNELIVKRYLKPGDIYVHADLHGATSCVIKNPSGEPIPPRTLTEAGTMALCYSAAWEARVVTSAWWVSHNQVSKTAPTGEYLTTGSFMIRGKKNFLPPSYLMMGFSFLFKVDESCVWRHREERKIKVQDEDLEMVSSSACELVSEEVELLGGDSSNEEEKAECQEAPEDVEAVSESNRDEGVADLDQGRVNTPPAPEGDSEEDDGESEDEVEHLESKSEVKEEEVNYPDTTIDLSHLQSQRSLQKIVPKEEEPNLSDSKPQGRRHLSAKERREMKKKKQQNDSENLEPPEEKEKETETQPPPAPNTNKGVPAPQPIKRGQKSKMKKMKEKYKDQDEEDRELIMKLLGSAGSNKEEKGKKGKKGKTKEEPAKKQQQKPKAAHCGAGRGKEMLPAGVLLHELQDPVLQEQQDEKEEQDQDQPGVEEGEALLDSLTGQPHPEDILLFAVPICAPYTAMMNYKYKVKLTPGTQKKGKAAKIALHNFMQSKEASAREKDLFRSVKDTDLSRNIPGKVKVSAPHLLNRKKK

Target ORF information:

RefSeq Version	XM_011587595.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis nuclear export mediator factor (NEMF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011587595.1

ORF Insert Sequence:

ATGAAGTCCC GCTTCAGCAC CGTCGACATC CGCGCGGTGC TCGCCGAGCT CCGGCAGAGC 
TTATTGGGAA TGAGAGTAAA CAATGTTTAT GATGTGGATA ATAAGACGTA TCTTATTCGC 
CTTCAAAAAC CAGACTGCAA GGCCACGCTG CTGCTTGAAT CTGGTATACG CATTCACACG 
ACAGAGTTCG AGTGGCCAAA AAACATGATG CCATCTGGCT TTGCAATGAA GTGCCGTAAG 
CATTTAAAGA CCAGAAGACT TGTCAGTGTA AAACAGCTGG GTATAGACAG AATTGTGGAC 
TTCCAGTTTG GAAGCGATGA AGCTGCTTAT CACCTGATTA TTGAGCTGTA CGACAGGGGT 
AACATTGTCC TGACAGACCA TGAATACGTC ATTTTAAACA TTCTGAGGTT TCGCACGAAT 
GAAGCGGATG ATGTCAGATT TGCGGTTCGG GAACGATACC CAGTCGACAG TGCAAAAGTG 
CCTGCACCTC TGCCAACCTT GGAAAGGTTA ACTGAAATGA TATCAAATGC ACCCAAAGGG 
GAACAACTGA AGAGGGCTCT TAACCCGCAT CTTCCTTATG GAGCCACTCT CATCGAGCAT 
TGCCTTATAG AAGCTGGATT TTCTGGGTAC GTCAAAATAG ATCAGCATAT GGAAAGTAAA 
GAAAATATTG AAAAAGTGCT TGCTGCTTTA GAGAAAGCAG AAGAATATAT GACACTAACT 
GACAACTTCA GTGGAAAGGG TTACATTATC CAGAAAAAGG AGAAAAAGCC AAGTCTGGAA 
CCAGATAAAC CAGCAGAAGA TATCTACACA TATGAGGAAT TTCATCCTTT CTTGTTTTCT 
CAACATTCAA AATGTCCGTA CTTGGAATTT GACTCATTCA ATAAGGCAGC AGATGAGTTC 
TACTCCAAGC TAGAGGGACA GAAAATTGAT CTGAAAGCAT TGCAGCAGGA AAAACAAGCA 
TTGAAGAAAC TTGAAAATGT CCGTAGGGAT CATGAGCACA GACTAGAAGC TCTTCAGCAA 
GCTCAGGAAG CTGATAAGAT GAAAGGAGAA CTTATAGAAA TGAACTTGGA GATTGTTGAC 
CGAGCCATCC AGGTGGTTCG TAGCGCGTTA GCCAACCAGA TAGACTGGAC AGAGATTGGA 
GCGATTGTTA AAGAAGCTCA AGCCCAGGGA GACCCCGTTG CAAGTGCAAT CAAAGAATTA 
AAGCTTCAGA CAAATCATAT CACAATGCTG CTGAGGAACC CATATGTGTT ATCTGAAGAG 
GAAGAGGAGG AGGAGGATGC TAATTTAGAG AAAGAAGAAA CGGAAGAACC AAAGGGAAAA 
AAGAAAAAGA ATAAAAACAA ACAGTTGAAG AAACCTCAGA AAAACAAACC GTCATTAGTT 
GATGTTGATC TCAGTCTGTC TGCATATGCC AATGCCAAAA AGTACTATGA TCACAAAAGA 
CATGCAGCTA AGAAAACTCA GAAGACAGTA GAAGCTGCAG AAAAGGCATT CAAATCAGCC 
GAGAAGAAGA CGAAGCAGAC ACTGAGGGAA GTTCAGACAG TTACCACCAT TCAGAAAGCG 
AGAAAGGTCT ATTGGTTTGA GAAGTTCCTG TGGTTCATTA GTTCTGAGAA TTACCTTATT 
ATTGCTGGAA GAGATCAACA GCAGAATGAG CTGATTGTAA AGCGGTATCT TAAACCGGGG 
GACATATACG TGCATGCCGA TCTCCACGGA GCCACAAGCT GTGTGATCAA GAACCCGTCA 
GGTGAACCAA TCCCTCCACG AACTCTGACA GAGGCAGGCA CGATGGCCTT GTGTTACAGC 
GCTGCCTGGG AAGCCCGTGT TGTCACCAGT GCTTGGTGGG TCTCCCACAA TCAGGTTTCT 
AAAACTGCAC CTACAGGAGA ATACCTCACT ACTGGAAGCT TCATGATCCG AGGGAAGAAG 
AATTTTCTTC CACCTTCCTA TCTGATGATG GGCTTTAGCT TCTTGTTTAA GGTGGATGAA 
AGTTGTGTTT GGAGACACCG AGAAGAAAGG AAGATCAAAG TACAGGATGA GGATCTAGAA 
ATGGTTTCCA GCAGTGCCTG CGAACTGGTG TCTGAAGAAG TGGAGCTATT AGGAGGAGAT 
AGCAGCAACG AAGAGGAAAA AGCAGAGTGT CAGGAAGCAC CAGAGGATGT GGAAGCCGTG 
TCTGAGAGCA ATCGTGATGA GGGCGTTGCT GACCTTGACC AGGGAAGAGT AAACACACCT 
CCTGCACCAG AGGGTGACTC CGAAGAGGAT GATGGAGAGT CTGAAGATGA AGTGGAACAT 
CTGGAGTCAA AGAGTGAAGT GAAGGAGGAA GAGGTAAATT ACCCAGATAC AACAATCGAC 
CTGTCGCATC TTCAGTCTCA AAGATCCCTG CAGAAAATCG TCCCCAAAGA GGAAGAACCC 
AACTTGAGTG ACAGCAAGCC CCAGGGGCGA AGGCACCTGT CTGCCAAGGA GAGGAGAGAA 
ATGAAGAAAA AGAAGCAGCA AAACGACTCT GAGAACTTGG AGCCGCCTGA AGAGAAGGAG 
AAAGAGACGG AAACACAGCC TCCCCCTGCT CCCAACACCA ATAAGGGTGT GCCGGCCCCT 
CAGCCCATCA AGCGGGGCCA GAAGAGTAAA ATGAAGAAGA TGAAGGAGAA GTACAAGGAC 
CAGGATGAGG AGGACCGGGA GCTCATCATG AAGCTGCTGG GGTCTGCAGG GTCAAACAAG 
GAGGAGAAAG GGAAAAAAGG GAAAAAGGGG AAGACAAAAG AAGAGCCAGC AAAGAAGCAA 
CAGCAGAAAC CCAAGGCTGC TCATTGTGGT GCTGGAAGGG GCAAGGAGAT GCTCCCAGCA 
GGAGTCCTGC TGCACGAGTT GCAGGACCCA GTCCTGCAGG AGCAGCAGGA TGAGAAGGAG 
GAGCAAGACC AGGATCAGCC GGGGGTTGAG GAAGGCGAGG CGTTGCTGGA CTCCCTGACG 
GGCCAGCCAC ACCCTGAGGA TATTCTGCTC TTTGCTGTTC CCATCTGTGC TCCCTACACG 
GCGATGATGA ACTATAAGTA TAAAGTCAAG CTCACTCCAG GCACCCAAAA GAAGGGAAAA 
GCTGCAAAGA TTGCCTTGCA TAATTTTATG CAATCCAAAG AAGCTAGTGC AAGAGAAAAA 
GACCTGTTCC GCAGTGTAAA GGATACCGAT TTGTCAAGAA ATATTCCTGG TAAAGTGAAA 
GTGTCTGCAC CTCATCTCCT GAACAGGAAA AAGAAGTGA