MAP3K5 cDNA ORF clone, Apteryx australis mantelli

Gene
Clones
gRNAs

Summary

Gene Symbol	MAP3K5
Entrez Gene ID	106497979
Full Name	mitogen-activated protein kinase kinase kinase 5
Gene Type	protein-coding
Organism	Apteryx australis mantelli

mRNA and Protein(s)

mRNA	Protein	Name
XM_013959042.1	XP_013814496.1	mitogen-activated protein kinase kinase kinase 5 isoform X1
XM_013959043.1	XP_013814497.1	mitogen-activated protein kinase kinase kinase 5 isoform X2

Pathways from BioSystems
Gene Ontology

The following MAP3K5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP3K5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAp26346	XM_013959042.1 Latest version!	Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAp26347	XM_013959043.1 Latest version!	Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAP3K5 ( XM_013959042.1 ) cDNA ORF clone, Apteryx australis mantelli -> XP_013814496.1 Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAp26346
Clone ID Related Accession (Same CDS sequence)	XM_013959042.1
Accession Version	XM_013959042.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-08
Organism	Apteryx australis mantelli
Product	mitogen-activated protein kinase kinase kinase 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014006019.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apteryx australis mantelli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTACCT TTGGTAGATC GATTTATTCA ACTCTTAAAG GTGGCGCAGG CCAGCTCAAG 
GATTATGATT CCATTGTGAA ACTGGTGAAA ACCTTAGAAA AACTGCCTAC TTTTGACTTG 
GCCTCCCACC ACCATGTGAG ATTTCATTAT GCATTTGCAC TGAACAGGCG AAATCTGCCT 
GGAGACAGAC AGAAAGCTCT AGAAATTATG GCTCCTCTGG TACAACAGGA AGACCAAGTA 
GCTTCAGATA TGTACTGCCT GGTTGGGCGA ATTTACAAAG ACCTATTTTT GGAATCTGGG 
TTCACAGATA TAGAAAGCAG AGACAAAGGG ACATTCTGGT TCAAGAAGGC GTTTGAGTCA 
GAGCCAACAT TACAGTCAGG AATTAATTAT GCAGTACTCC TCCTGGCAGC TGGACATCAC 
TTTGATACTT CTTTTGAGCT GCGGAAAGTT GGGGTAAAGA TCAGCAGCCT GCTTGGTAAA 
AAGGGGAGCT TGGAGAAACT GCAGAGCTAT TGGGAAGTGG GATCTTTCTT GGGGGCCAGT 
ATGTTGGCTA ACGACCACAT AAGAGTGATC CAGGCTTCAG AAAAGCTGTT TAAACTAAAG 
GCACCAGCAT GGTATCTGAA GTCTATTGTA GAGACCATTT TGATATATCA GCATTTTAAG 
AAACTGAATC CAGAACAGCA TACAGCCAAA CAGGAACTTG TTGACTTCTG GATGGACTTC 
CTTGTTGAAG CCACAAAGAC AGATGTGTCT GTAGTTAGAT TTCCAGTGTT AATATTAGAA 
CCGACAAAAA TCTATCAGCC TTCATATCTC TCCATCAACA GTGAAGCTGA GGAGAAGACT 
GTATCTATCT GGCATGTGCT GCCTGATGAC AAGAAAGGTA TTCATGAGTG GAACTTCAGT 
GCTGCTTCTA TCAGGGGAGT AAGCATTTCG AAGTTTGAAG AACGGTGCTG TTTCCTCTAT 
GTGCTGCACA ACTCTGATGA CTTTCAAATC TATTTCTGCA CAGAACTTCA CTGCAGGAGG 
TTTTTTGACA TGGTGAACAG TATAACTGAA GAAATAGGGA AGAATACAGA GGATGGGGAA 
TGTGATGGAG ACTCTCTGGA GTATGATTAT GAATATGATG AAAATGGCGA GCGAGTCATT 
CTGGGGAAAG GAACTTACGG CATTGTATAT GCAGGACGAG ATTTGAGCAA TCAAGTCAGA 
ATTGCTATTA AAGAAATCCC AGAGAGAGAT AGCAGGTACT CCCAACCTCT ACATGAAGAA 
ATAGCTTTGC ATAAGCATCT CAAACATAAA AATATTGTCC AGTATTTAGG TTCTCTTAGT 
GAGAATGGCT TCATTAAAAT ATTCATGGAA CAGGTGCCAG GTGGCAGCCT TTCTGCTCTT 
CTACGATCAA AATGGGGACC ATTAAAAAAT AATGAGCAGA CAATTGGCTT TTACACCAAG 
CAGATTCTTG AAGGATTAAA ATACCTCCAT GATAATCAGA TAGTGCATCG AGACATAAAG 
GGTGATAACG TGCTTATCAA CACTTACAGT GGAGTGTTAA AGATCTCAGA CTTTGGAACT 
TCGAAGAGAC TTGCAGGAAT CAACCCATGT ACTGAAACTT TCACTGGTAC CCTTCAGTAT 
ATGGCACCGG AAATCATAGA CAAAGGCCCA CGTGGCTATG GGAAAGCTGC AGACATCTGG 
TCTTTGGGAT GCACAATTAT TGAAATGGCT ACAGGGAAAC CCCCATTTTA TGAACTGGGA 
GAACCACAAG CAGCTATGTT TAAGGTGGGA ATGTTTAAAA TACACCCAGA GATTCCTGAA 
TCCATGTCTG CAGAAGCCAA GGCTTTCATA CTGCGTTGCT TTGAACCAGA TCCTGATAAA 
CGAGCTTTTG CTCATGAGCT ACTTATAGAT GAATTCTTGA AGGTTTCGAG CAGAAAAAGA 
AAGTCCCCAT CAAAGCTCTC AGTTCTTTCA GCTAGCTCAA ATGAGTATCT TCGGAGTATA 
TCCTTACCTG TTCCTGTTCT GGTTGAAGAT ACAAGTAGCA GCAGTGAATA TGGCTCTGTT 
TCACCTGATA CAGAATTAAA AATTGATCCA TTCTCTTTCA AAACAAGAGC TAAATCCTGT 
GGAGAAAAAG ATGGGAAGGG AATCCGGTCT CTTTTTCTGA GCATCCCAGA TGAGAATTTT 
GAAGATCACA GTGCACCTCC TTCACCTGAA GAAAAAGATT CTGGGTTTTT CTTGCTGAAG 
AAAGATAGTG AAAGGAGGGC CACCCTTCAT AGGATATTAA CTGAGGACCA AGAAAAGGTG 
GTGAGAAACT TGATGGAAGC TTTGGCTCAG GGAGCTGAAG AGCCCAAGCT AAAATGCGAA 
CATATCACAA CGCTTGTTTC CAGCCTCAGA GAATTTGTAC GGTCCACTGA TCGCAAAATC 
ATCGCAACCA CTCTTTCAAA ACTGAAACTG GAGTTAGACT TTGACAGCCA TGGTATCAGT 
CAAGTGCAGC TGGTGCTGTT TGGTTTTCAA GATGCTGTCA ACAAAGTTCT CCGAAATCAC 
AACATCAAGC CACATTGGAT GTTTGCATTG GACAGCATAA TTAGAAAAGC TGTGCAGACT 
GCTATTACTA TTCTGGTCCC AGAATTGAGG CCACATTTTA GTCTTGCATC TGAAAGTGAT 
ACAGCAGATC AGGATGATAT AGAAGTTGAA GAAGATCAAG ATGAACAGCC TCAGAATCAA 
ACTATCCAGC CTTGCATTGT CATGGAAGAT GCAGTAGCTA CCTCAGGTGT GAGCACGCTC 
AGCTCTACGG TATCCCATGA CTCTCAGGCT GTTCATAGAT CACTGAGTGT CCAGCTGGGG 
AGGCTAAAAT TAGAGACAAA CAGGCTATTG GAGGAGTTGG TTCAAAAGGA GAGAGAATTT 
CAAGTCCTCT TACATCAAGC TGTAGAAGAA AAAGATCAAG AGATCAAAAA CCTGCGTCTT 
AGGTCACAGC CAATACATAT TCCTGGATTG TCTGTCTGTC ATCGTCATTC CACTGGCGCA 
GGGACCGAAG ACCCTGATCT CATAAACTGG CTGAGTGTTC AAGGAGCTGA TGAGGATACT 
ATAAACAGGT TCTTGGCTGA AGACTATACA TTAATGGATG TTCTCTGCTG TGTTACGCGA 
GATGATCTGA AATGTTTGAG ACTGAGAGGA GGGATGCTAT GCACGCTCTG GAAGGCCATC 
ACTGACTTTC GAGAGAAACA CGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013814496.1
CDS	87..3293
Translation	MFTFGRSIYSTLKGGAGQLKDYDSIVKLVKTLEKLPTFDLASHHHVRFHYAFALNRRNLPGDRQKALEIMAPLVQQEDQVASDMYCLVGRIYKDLFLESGFTDIESRDKGTFWFKKAFESEPTLQSGINYAVLLLAAGHHFDTSFELRKVGVKISSLLGKKGSLEKLQSYWEVGSFLGASMLANDHIRVIQASEKLFKLKAPAWYLKSIVETILIYQHFKKLNPEQHTAKQELVDFWMDFLVEATKTDVSVVRFPVLILEPTKIYQPSYLSINSEAEEKTVSIWHVLPDDKKGIHEWNFSAASIRGVSISKFEERCCFLYVLHNSDDFQIYFCTELHCRRFFDMVNSITEEIGKNTEDGECDGDSLEYDYEYDENGERVILGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSLSENGFIKIFMEQVPGGSLSALLRSKWGPLKNNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKIHPEIPESMSAEAKAFILRCFEPDPDKRAFAHELLIDEFLKVSSRKRKSPSKLSVLSASSNEYLRSISLPVPVLVEDTSSSSEYGSVSPDTELKIDPFSFKTRAKSCGEKDGKGIRSLFLSIPDENFEDHSAPPSPEEKDSGFFLLKKDSERRATLHRILTEDQEKVVRNLMEALAQGAEEPKLKCEHITTLVSSLREFVRSTDRKIIATTLSKLKLELDFDSHGISQVQLVLFGFQDAVNKVLRNHNIKPHWMFALDSIIRKAVQTAITILVPELRPHFSLASESDTADQDDIEVEEDQDEQPQNQTIQPCIVMEDAVATSGVSTLSSTVSHDSQAVHRSLSVQLGRLKLETNRLLEELVQKEREFQVLLHQAVEEKDQEIKNLRLRSQPIHIPGLSVCHRHSTGAGTEDPDLINWLSVQGADEDTINRFLAEDYTLMDVLCCVTRDDLKCLRLRGGMLCTLWKAITDFREKHA

Target ORF information:

RefSeq Version	XM_013959042.1
Organism	Apteryx australis mantelli
Definition	Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013959042.1

ORF Insert Sequence:

ATGTTTACCT TTGGTAGATC GATTTATTCA ACTCTTAAAG GTGGCGCAGG CCAGCTCAAG 
GATTATGATT CCATTGTGAA ACTGGTGAAA ACCTTAGAAA AACTGCCTAC TTTTGACTTG 
GCCTCCCACC ACCATGTGAG ATTTCATTAT GCATTTGCAC TGAACAGGCG AAATCTGCCT 
GGAGACAGAC AGAAAGCTCT AGAAATTATG GCTCCTCTGG TACAACAGGA AGACCAAGTA 
GCTTCAGATA TGTACTGCCT GGTTGGGCGA ATTTACAAAG ACCTATTTTT GGAATCTGGG 
TTCACAGATA TAGAAAGCAG AGACAAAGGG ACATTCTGGT TCAAGAAGGC GTTTGAGTCA 
GAGCCAACAT TACAGTCAGG AATTAATTAT GCAGTACTCC TCCTGGCAGC TGGACATCAC 
TTTGATACTT CTTTTGAGCT GCGGAAAGTT GGGGTAAAGA TCAGCAGCCT GCTTGGTAAA 
AAGGGGAGCT TGGAGAAACT GCAGAGCTAT TGGGAAGTGG GATCTTTCTT GGGGGCCAGT 
ATGTTGGCTA ACGACCACAT AAGAGTGATC CAGGCTTCAG AAAAGCTGTT TAAACTAAAG 
GCACCAGCAT GGTATCTGAA GTCTATTGTA GAGACCATTT TGATATATCA GCATTTTAAG 
AAACTGAATC CAGAACAGCA TACAGCCAAA CAGGAACTTG TTGACTTCTG GATGGACTTC 
CTTGTTGAAG CCACAAAGAC AGATGTGTCT GTAGTTAGAT TTCCAGTGTT AATATTAGAA 
CCGACAAAAA TCTATCAGCC TTCATATCTC TCCATCAACA GTGAAGCTGA GGAGAAGACT 
GTATCTATCT GGCATGTGCT GCCTGATGAC AAGAAAGGTA TTCATGAGTG GAACTTCAGT 
GCTGCTTCTA TCAGGGGAGT AAGCATTTCG AAGTTTGAAG AACGGTGCTG TTTCCTCTAT 
GTGCTGCACA ACTCTGATGA CTTTCAAATC TATTTCTGCA CAGAACTTCA CTGCAGGAGG 
TTTTTTGACA TGGTGAACAG TATAACTGAA GAAATAGGGA AGAATACAGA GGATGGGGAA 
TGTGATGGAG ACTCTCTGGA GTATGATTAT GAATATGATG AAAATGGCGA GCGAGTCATT 
CTGGGGAAAG GAACTTACGG CATTGTATAT GCAGGACGAG ATTTGAGCAA TCAAGTCAGA 
ATTGCTATTA AAGAAATCCC AGAGAGAGAT AGCAGGTACT CCCAACCTCT ACATGAAGAA 
ATAGCTTTGC ATAAGCATCT CAAACATAAA AATATTGTCC AGTATTTAGG TTCTCTTAGT 
GAGAATGGCT TCATTAAAAT ATTCATGGAA CAGGTGCCAG GTGGCAGCCT TTCTGCTCTT 
CTACGATCAA AATGGGGACC ATTAAAAAAT AATGAGCAGA CAATTGGCTT TTACACCAAG 
CAGATTCTTG AAGGATTAAA ATACCTCCAT GATAATCAGA TAGTGCATCG AGACATAAAG 
GGTGATAACG TGCTTATCAA CACTTACAGT GGAGTGTTAA AGATCTCAGA CTTTGGAACT 
TCGAAGAGAC TTGCAGGAAT CAACCCATGT ACTGAAACTT TCACTGGTAC CCTTCAGTAT 
ATGGCACCGG AAATCATAGA CAAAGGCCCA CGTGGCTATG GGAAAGCTGC AGACATCTGG 
TCTTTGGGAT GCACAATTAT TGAAATGGCT ACAGGGAAAC CCCCATTTTA TGAACTGGGA 
GAACCACAAG CAGCTATGTT TAAGGTGGGA ATGTTTAAAA TACACCCAGA GATTCCTGAA 
TCCATGTCTG CAGAAGCCAA GGCTTTCATA CTGCGTTGCT TTGAACCAGA TCCTGATAAA 
CGAGCTTTTG CTCATGAGCT ACTTATAGAT GAATTCTTGA AGGTTTCGAG CAGAAAAAGA 
AAGTCCCCAT CAAAGCTCTC AGTTCTTTCA GCTAGCTCAA ATGAGTATCT TCGGAGTATA 
TCCTTACCTG TTCCTGTTCT GGTTGAAGAT ACAAGTAGCA GCAGTGAATA TGGCTCTGTT 
TCACCTGATA CAGAATTAAA AATTGATCCA TTCTCTTTCA AAACAAGAGC TAAATCCTGT 
GGAGAAAAAG ATGGGAAGGG AATCCGGTCT CTTTTTCTGA GCATCCCAGA TGAGAATTTT 
GAAGATCACA GTGCACCTCC TTCACCTGAA GAAAAAGATT CTGGGTTTTT CTTGCTGAAG 
AAAGATAGTG AAAGGAGGGC CACCCTTCAT AGGATATTAA CTGAGGACCA AGAAAAGGTG 
GTGAGAAACT TGATGGAAGC TTTGGCTCAG GGAGCTGAAG AGCCCAAGCT AAAATGCGAA 
CATATCACAA CGCTTGTTTC CAGCCTCAGA GAATTTGTAC GGTCCACTGA TCGCAAAATC 
ATCGCAACCA CTCTTTCAAA ACTGAAACTG GAGTTAGACT TTGACAGCCA TGGTATCAGT 
CAAGTGCAGC TGGTGCTGTT TGGTTTTCAA GATGCTGTCA ACAAAGTTCT CCGAAATCAC 
AACATCAAGC CACATTGGAT GTTTGCATTG GACAGCATAA TTAGAAAAGC TGTGCAGACT 
GCTATTACTA TTCTGGTCCC AGAATTGAGG CCACATTTTA GTCTTGCATC TGAAAGTGAT 
ACAGCAGATC AGGATGATAT AGAAGTTGAA GAAGATCAAG ATGAACAGCC TCAGAATCAA 
ACTATCCAGC CTTGCATTGT CATGGAAGAT GCAGTAGCTA CCTCAGGTGT GAGCACGCTC 
AGCTCTACGG TATCCCATGA CTCTCAGGCT GTTCATAGAT CACTGAGTGT CCAGCTGGGG 
AGGCTAAAAT TAGAGACAAA CAGGCTATTG GAGGAGTTGG TTCAAAAGGA GAGAGAATTT 
CAAGTCCTCT TACATCAAGC TGTAGAAGAA AAAGATCAAG AGATCAAAAA CCTGCGTCTT 
AGGTCACAGC CAATACATAT TCCTGGATTG TCTGTCTGTC ATCGTCATTC CACTGGCGCA 
GGGACCGAAG ACCCTGATCT CATAAACTGG CTGAGTGTTC AAGGAGCTGA TGAGGATACT 
ATAAACAGGT TCTTGGCTGA AGACTATACA TTAATGGATG TTCTCTGCTG TGTTACGCGA 
GATGATCTGA AATGTTTGAG ACTGAGAGGA GGGATGCTAT GCACGCTCTG GAAGGCCATC 
ACTGACTTTC GAGAGAAACA CGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K5 ( XM_013959043.1 ) cDNA ORF clone, Apteryx australis mantelli -> XP_013814497.1 Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAp26347
Clone ID Related Accession (Same CDS sequence)	XM_013959043.1
Accession Version	XM_013959043.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-08
Organism	Apteryx australis mantelli
Product	mitogen-activated protein kinase kinase kinase 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014006019.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apteryx australis mantelli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGATG CATTCCGCCA GCCTTCCCTG TTCTACCACC TGGGAGTCAG AGAGAGCTTC 
AGTATGGCTA ACAACATTAT CCTCTACTGT GATACAAATG CTGAGTCTCT GCAGTCACTG 
AAGGAAATTA TTTGCCAGAA GAATAATATG TGTACTGGTA ACTACATATT TGTTCCTTAT 
ATGATAACTC CACACAACAA GGTGTACTGC TGCGATAGTA GTTTTATGAA GGGACTAACA 
GAACTGATGC AGCCCAGTTT TGAGTCGCTG CTTGGGCCTA TATGTTTACC TTTGGTAGAT 
CGATTTATTC AACTCTTAAA GGTGGCGCAG GCCAGCTCAA GCCAGTATTT CCGGCAATCC 
ATTCTTAATG ATATCAGAAA GGCCCGTACC CTCTACACAG GCAAAGAACT TGCAGCAGAG 
CTGGCAAGGA TTCGACAGCG AGTGGATAAT ATTGAAGTGT TGTCTGCTGA CATTGTAATA 
AACTTGCTGC TGTCCTACAG AGACATTCAG GATTATGATT CCATTGTGAA ACTGGTGAAA 
ACCTTAGAAA AACTGCCTAC TTTTGACTTG GCCTCCCACC ACCATGTGAG ATTTCATTAT 
GCATTTGCAC TGAACAGGCG AAATCTGCCT GGAGACAGAC AGAAAGCTCT AGAAATTATG 
GCTCCTCTGG TACAACAGGA AGACCAAGTA GCTTCAGATA TGTACTGCCT GGTTGGGCGA 
ATTTACAAAG ACCTATTTTT GGAATCTGGG TTCACAGATA TAGAAAGCAG AGACAAAGGG 
ACATTCTGGT TCAAGAAGGC GTTTGAGTCA GAGCCAACAT TACAGTCAGG AATTAATTAT 
GCAGTACTCC TCCTGGCAGC TGGACATCAC TTTGATACTT CTTTTGAGCT GCGGAAAGTT 
GGGGTAAAGA TCAGCAGCCT GCTTGGTAAA AAGGGGAGCT TGGAGAAACT GCAGAGCTAT 
TGGGAAGTGG GATCTTTCTT GGGGGCCAGT ATGTTGGCTA ACGACCACAT AAGAGTGATC 
CAGGCTTCAG AAAAGCTGTT TAAACTAAAG GCACCAGCAT GGTATCTGAA GTCTATTGTA 
GAGACCATTT TGATATATCA GCATTTTAAG AAACTGAATC CAGAACAGCA TACAGCCAAA 
CAGGAACTTG TTGACTTCTG GATGGACTTC CTTGTTGAAG CCACAAAGAC AGATGTGTCT 
GTAGTTAGAT TTCCAGTGTT AATATTAGAA CCGACAAAAA TCTATCAGCC TTCATATCTC 
TCCATCAACA GTGAAGCTGA GGAGAAGACT GTATCTATCT GGCATGTGCT GCCTGATGAC 
AAGAAAGGTA TTCATGAGTG GAACTTCAGT GCTGCTTCTA TCAGGGGAGT AAGCATTTCG 
AAGTTTGAAG AACGGTGCTG TTTCCTCTAT GTGCTGCACA ACTCTGATGA CTTTCAAATC 
TATTTCTGCA CAGAACTTCA CTGCAGGAGG TTTTTTGACA TGGTGAACAG TATAACTGAA 
GAAATAGGGA AGAATACAGA GGATGGGGAA TGTGATGGAG ACTCTCTGGA GTATGATTAT 
GAATATGATG AAAATGGCGA GCGAGTCATT CTGGGGAAAG GAACTTACGG CATTGTATAT 
GCAGGACGAG ATTTGAGCAA TCAAGTCAGA ATTGCTATTA AAGAAATCCC AGAGAGAGAT 
AGCAGGTACT CCCAACCTCT ACATGAAGAA ATAGCTTTGC ATAAGCATCT CAAACATAAA 
AATATTGTCC AGTATTTAGG TTCTCTTAGT GAGAATGGCT TCATTAAAAT ATTCATGGAA 
CAGGTGCCAG GTGGCAGCCT TTCTGCTCTT CTACGATCAA AATGGGGACC ATTAAAAAAT 
AATGAGCAGA CAATTGGCTT TTACACCAAG CAGATTCTTG AAGGATTAAA ATACCTCCAT 
GATAATCAGA TAGTGCATCG AGACATAAAG GGTGATAACG TGCTTATCAA CACTTACAGT 
GGAGTGTTAA AGATCTCAGA CTTTGGAACT TCGAAGAGAC TTGCAGGAAT CAACCCATGT 
ACTGAAACTT TCACTGGTAC CCTTCAGTAT ATGGCACCGG AAATCATAGA CAAAGGCCCA 
CGTGGCTATG GGAAAGCTGC AGACATCTGG TCTTTGGGAT GCACAATTAT TGAAATGGCT 
ACAGGGAAAC CCCCATTTTA TGAACTGGGA GAACCACAAG CAGCTATGTT TAAGGTGGGA 
ATGTTTAAAA TACACCCAGA GATTCCTGAA TCCATGTCTG CAGAAGCCAA GGCTTTCATA 
CTGCGTTGCT TTGAACCAGA TCCTGATAAA CGAGCTTTTG CTCATGAGCT ACTTATAGAT 
GAATTCTTGA AGGTTTCGAG CAGAAAAAGA AAGTCCCCAT CAAAGCTCTC AGTTCTTTCA 
GCTAGCTCAA ATGAGTATCT TCGGAGTATA TCCTTACCTG TTCCTGTTCT GGTTGAAGAT 
ACAAGTAGCA GCAGTGAATA TGGCTCTGTT TCACCTGATA CAGAATTAAA AATTGATCCA 
TTCTCTTTCA AAACAAGAGC TAAATCCTGT GGAGAAAAAG ATGGGAAGGG AATCCGGTCT 
CTTTTTCTGA GCATCCCAGA TGAGAATTTT GAAGATCACA GTGCACCTCC TTCACCTGAA 
GAAAAAGATT CTGGGTTTTT CTTGCTGAAG AAAGATAGTG AAAGGAGGGC CACCCTTCAT 
AGGATATTAA CTGAGGACCA AGAAAAGGTG GTGAGAAACT TGATGGAAGC TTTGGCTCAG 
GGAGCTGAAG AGCCCAAGCT AAAATGCGAA CATATCACAA CGCTTGTTTC CAGCCTCAGA 
GAATTTGTAC GGTCCACTGA TCGCAAAATC ATCGCAACCA CTCTTTCAAA ACTGAAACTG 
GAGTTAGACT TTGACAGCCA TGGTATCAGT CAAGTGCAGC TGGTGCTGTT TGGTTTTCAA 
GATGCTGTCA ACAAAGTTCT CCGAAATCAC AACATCAAGC CACATTGGAT GTTTGCATTG 
GACAGCATAA TTAGAAAAGC TGTGCAGACT GCTATTACTA TTCTGGTCCC AGAATTGAGG 
CCACATTTTA GTCTTGCATC TGAAAGTGAT ACAGCAGATC AGGATGATAT AGAAGTTGAA 
GAAGATCAAG ATGAACAGCC TCAGAATCAA ACTATCCAGC CTTGCATTGT CATGGAAGAT 
GCAGTAGCTA CCTCAGGTGT GAGCACGCTC AGCTCTACGG TATCCCATGA CTCTCAGGCT 
GTTCATAGAT CACTGAGTGT CCAGCTGGGG AGGCTAAAAT TAGAGACAAA CAGGCTATTG 
GAGGAGTTGG TTCAAAAGGA GAGAGAATTT CAAGTCCTCT TACATCAAGC TGTAGAAGAA 
AAAGATCAAG AGATCAAAAA CCTGCGTCTT AGGTCACAGC CAATACATAT TCCTGGATTG 
TCTGTCTGTC ATCGTCATTC CACTGGCGCA GGGACCGAAG ACCCTGATCT CATAAACTGG 
CTGAGTGTTC AAGGAGCTGA TGAGGATACT ATAAACAGGT TCTTGGCTGA AGACTATACA 
TTAATGGATG TTCTCTGCTG TGTTACGCGA GATGATCTGA AATGTTTGAG ACTGAGAGGA 
GGGATGCTAT GCACGCTCTG GAAGGCCATC ACTGACTTTC GAGAGAAACA CGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013814497.1
CDS	19..3675
Translation	MSDAFRQPSLFYHLGVRESFSMANNIILYCDTNAESLQSLKEIICQKNNMCTGNYIFVPYMITPHNKVYCCDSSFMKGLTELMQPSFESLLGPICLPLVDRFIQLLKVAQASSSQYFRQSILNDIRKARTLYTGKELAAELARIRQRVDNIEVLSADIVINLLLSYRDIQDYDSIVKLVKTLEKLPTFDLASHHHVRFHYAFALNRRNLPGDRQKALEIMAPLVQQEDQVASDMYCLVGRIYKDLFLESGFTDIESRDKGTFWFKKAFESEPTLQSGINYAVLLLAAGHHFDTSFELRKVGVKISSLLGKKGSLEKLQSYWEVGSFLGASMLANDHIRVIQASEKLFKLKAPAWYLKSIVETILIYQHFKKLNPEQHTAKQELVDFWMDFLVEATKTDVSVVRFPVLILEPTKIYQPSYLSINSEAEEKTVSIWHVLPDDKKGIHEWNFSAASIRGVSISKFEERCCFLYVLHNSDDFQIYFCTELHCRRFFDMVNSITEEIGKNTEDGECDGDSLEYDYEYDENGERVILGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSLSENGFIKIFMEQVPGGSLSALLRSKWGPLKNNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKIHPEIPESMSAEAKAFILRCFEPDPDKRAFAHELLIDEFLKVSSRKRKSPSKLSVLSASSNEYLRSISLPVPVLVEDTSSSSEYGSVSPDTELKIDPFSFKTRAKSCGEKDGKGIRSLFLSIPDENFEDHSAPPSPEEKDSGFFLLKKDSERRATLHRILTEDQEKVVRNLMEALAQGAEEPKLKCEHITTLVSSLREFVRSTDRKIIATTLSKLKLELDFDSHGISQVQLVLFGFQDAVNKVLRNHNIKPHWMFALDSIIRKAVQTAITILVPELRPHFSLASESDTADQDDIEVEEDQDEQPQNQTIQPCIVMEDAVATSGVSTLSSTVSHDSQAVHRSLSVQLGRLKLETNRLLEELVQKEREFQVLLHQAVEEKDQEIKNLRLRSQPIHIPGLSVCHRHSTGAGTEDPDLINWLSVQGADEDTINRFLAEDYTLMDVLCCVTRDDLKCLRLRGGMLCTLWKAITDFREKHA

Target ORF information:

RefSeq Version	XM_013959043.1
Organism	Apteryx australis mantelli
Definition	Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013959043.1

ORF Insert Sequence:

ATGAGTGATG CATTCCGCCA GCCTTCCCTG TTCTACCACC TGGGAGTCAG AGAGAGCTTC 
AGTATGGCTA ACAACATTAT CCTCTACTGT GATACAAATG CTGAGTCTCT GCAGTCACTG 
AAGGAAATTA TTTGCCAGAA GAATAATATG TGTACTGGTA ACTACATATT TGTTCCTTAT 
ATGATAACTC CACACAACAA GGTGTACTGC TGCGATAGTA GTTTTATGAA GGGACTAACA 
GAACTGATGC AGCCCAGTTT TGAGTCGCTG CTTGGGCCTA TATGTTTACC TTTGGTAGAT 
CGATTTATTC AACTCTTAAA GGTGGCGCAG GCCAGCTCAA GCCAGTATTT CCGGCAATCC 
ATTCTTAATG ATATCAGAAA GGCCCGTACC CTCTACACAG GCAAAGAACT TGCAGCAGAG 
CTGGCAAGGA TTCGACAGCG AGTGGATAAT ATTGAAGTGT TGTCTGCTGA CATTGTAATA 
AACTTGCTGC TGTCCTACAG AGACATTCAG GATTATGATT CCATTGTGAA ACTGGTGAAA 
ACCTTAGAAA AACTGCCTAC TTTTGACTTG GCCTCCCACC ACCATGTGAG ATTTCATTAT 
GCATTTGCAC TGAACAGGCG AAATCTGCCT GGAGACAGAC AGAAAGCTCT AGAAATTATG 
GCTCCTCTGG TACAACAGGA AGACCAAGTA GCTTCAGATA TGTACTGCCT GGTTGGGCGA 
ATTTACAAAG ACCTATTTTT GGAATCTGGG TTCACAGATA TAGAAAGCAG AGACAAAGGG 
ACATTCTGGT TCAAGAAGGC GTTTGAGTCA GAGCCAACAT TACAGTCAGG AATTAATTAT 
GCAGTACTCC TCCTGGCAGC TGGACATCAC TTTGATACTT CTTTTGAGCT GCGGAAAGTT 
GGGGTAAAGA TCAGCAGCCT GCTTGGTAAA AAGGGGAGCT TGGAGAAACT GCAGAGCTAT 
TGGGAAGTGG GATCTTTCTT GGGGGCCAGT ATGTTGGCTA ACGACCACAT AAGAGTGATC 
CAGGCTTCAG AAAAGCTGTT TAAACTAAAG GCACCAGCAT GGTATCTGAA GTCTATTGTA 
GAGACCATTT TGATATATCA GCATTTTAAG AAACTGAATC CAGAACAGCA TACAGCCAAA 
CAGGAACTTG TTGACTTCTG GATGGACTTC CTTGTTGAAG CCACAAAGAC AGATGTGTCT 
GTAGTTAGAT TTCCAGTGTT AATATTAGAA CCGACAAAAA TCTATCAGCC TTCATATCTC 
TCCATCAACA GTGAAGCTGA GGAGAAGACT GTATCTATCT GGCATGTGCT GCCTGATGAC 
AAGAAAGGTA TTCATGAGTG GAACTTCAGT GCTGCTTCTA TCAGGGGAGT AAGCATTTCG 
AAGTTTGAAG AACGGTGCTG TTTCCTCTAT GTGCTGCACA ACTCTGATGA CTTTCAAATC 
TATTTCTGCA CAGAACTTCA CTGCAGGAGG TTTTTTGACA TGGTGAACAG TATAACTGAA 
GAAATAGGGA AGAATACAGA GGATGGGGAA TGTGATGGAG ACTCTCTGGA GTATGATTAT 
GAATATGATG AAAATGGCGA GCGAGTCATT CTGGGGAAAG GAACTTACGG CATTGTATAT 
GCAGGACGAG ATTTGAGCAA TCAAGTCAGA ATTGCTATTA AAGAAATCCC AGAGAGAGAT 
AGCAGGTACT CCCAACCTCT ACATGAAGAA ATAGCTTTGC ATAAGCATCT CAAACATAAA 
AATATTGTCC AGTATTTAGG TTCTCTTAGT GAGAATGGCT TCATTAAAAT ATTCATGGAA 
CAGGTGCCAG GTGGCAGCCT TTCTGCTCTT CTACGATCAA AATGGGGACC ATTAAAAAAT 
AATGAGCAGA CAATTGGCTT TTACACCAAG CAGATTCTTG AAGGATTAAA ATACCTCCAT 
GATAATCAGA TAGTGCATCG AGACATAAAG GGTGATAACG TGCTTATCAA CACTTACAGT 
GGAGTGTTAA AGATCTCAGA CTTTGGAACT TCGAAGAGAC TTGCAGGAAT CAACCCATGT 
ACTGAAACTT TCACTGGTAC CCTTCAGTAT ATGGCACCGG AAATCATAGA CAAAGGCCCA 
CGTGGCTATG GGAAAGCTGC AGACATCTGG TCTTTGGGAT GCACAATTAT TGAAATGGCT 
ACAGGGAAAC CCCCATTTTA TGAACTGGGA GAACCACAAG CAGCTATGTT TAAGGTGGGA 
ATGTTTAAAA TACACCCAGA GATTCCTGAA TCCATGTCTG CAGAAGCCAA GGCTTTCATA 
CTGCGTTGCT TTGAACCAGA TCCTGATAAA CGAGCTTTTG CTCATGAGCT ACTTATAGAT 
GAATTCTTGA AGGTTTCGAG CAGAAAAAGA AAGTCCCCAT CAAAGCTCTC AGTTCTTTCA 
GCTAGCTCAA ATGAGTATCT TCGGAGTATA TCCTTACCTG TTCCTGTTCT GGTTGAAGAT 
ACAAGTAGCA GCAGTGAATA TGGCTCTGTT TCACCTGATA CAGAATTAAA AATTGATCCA 
TTCTCTTTCA AAACAAGAGC TAAATCCTGT GGAGAAAAAG ATGGGAAGGG AATCCGGTCT 
CTTTTTCTGA GCATCCCAGA TGAGAATTTT GAAGATCACA GTGCACCTCC TTCACCTGAA 
GAAAAAGATT CTGGGTTTTT CTTGCTGAAG AAAGATAGTG AAAGGAGGGC CACCCTTCAT 
AGGATATTAA CTGAGGACCA AGAAAAGGTG GTGAGAAACT TGATGGAAGC TTTGGCTCAG 
GGAGCTGAAG AGCCCAAGCT AAAATGCGAA CATATCACAA CGCTTGTTTC CAGCCTCAGA 
GAATTTGTAC GGTCCACTGA TCGCAAAATC ATCGCAACCA CTCTTTCAAA ACTGAAACTG 
GAGTTAGACT TTGACAGCCA TGGTATCAGT CAAGTGCAGC TGGTGCTGTT TGGTTTTCAA 
GATGCTGTCA ACAAAGTTCT CCGAAATCAC AACATCAAGC CACATTGGAT GTTTGCATTG 
GACAGCATAA TTAGAAAAGC TGTGCAGACT GCTATTACTA TTCTGGTCCC AGAATTGAGG 
CCACATTTTA GTCTTGCATC TGAAAGTGAT ACAGCAGATC AGGATGATAT AGAAGTTGAA 
GAAGATCAAG ATGAACAGCC TCAGAATCAA ACTATCCAGC CTTGCATTGT CATGGAAGAT 
GCAGTAGCTA CCTCAGGTGT GAGCACGCTC AGCTCTACGG TATCCCATGA CTCTCAGGCT 
GTTCATAGAT CACTGAGTGT CCAGCTGGGG AGGCTAAAAT TAGAGACAAA CAGGCTATTG 
GAGGAGTTGG TTCAAAAGGA GAGAGAATTT CAAGTCCTCT TACATCAAGC TGTAGAAGAA 
AAAGATCAAG AGATCAAAAA CCTGCGTCTT AGGTCACAGC CAATACATAT TCCTGGATTG 
TCTGTCTGTC ATCGTCATTC CACTGGCGCA GGGACCGAAG ACCCTGATCT CATAAACTGG 
CTGAGTGTTC AAGGAGCTGA TGAGGATACT ATAAACAGGT TCTTGGCTGA AGACTATACA 
TTAATGGATG TTCTCTGCTG TGTTACGCGA GATGATCTGA AATGTTTGAG ACTGAGAGGA 
GGGATGCTAT GCACGCTCTG GAAGGCCATC ACTGACTTTC GAGAGAAACA CGCCTGA