MAP3K4 gene cDNA ORF clone, Apteryx australis mantelli

mRNA	Protein	Name
XM_013945444.1	XP_013800898.1	mitogen-activated protein kinase kinase kinase 4 isoform X3
XM_013945450.1	XP_013800904.1	mitogen-activated protein kinase kinase kinase 4 isoform X9
XM_013945442.1	XP_013800896.1	mitogen-activated protein kinase kinase kinase 4 isoform X1
XM_013945443.1	XP_013800897.1	mitogen-activated protein kinase kinase kinase 4 isoform X2
XM_013945445.1	XP_013800899.1	mitogen-activated protein kinase kinase kinase 4 isoform X4
XM_013945446.1	XP_013800900.1	mitogen-activated protein kinase kinase kinase 4 isoform X5
XM_013945448.1	XP_013800902.1	mitogen-activated protein kinase kinase kinase 4 isoform X7
XM_013945449.1	XP_013800903.1	mitogen-activated protein kinase kinase kinase 4 isoform X8
XM_013945447.1	XP_013800901.1	mitogen-activated protein kinase kinase kinase 4 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAp16197	XM_013945448.1 Latest version!	Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OAp16198	XM_013945449.1 Latest version!	Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OAp16199	XM_013945450.1 Latest version!	Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OAp16191	XM_013945442.1 Latest version!	Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAp16192	XM_013945443.1 Latest version!	Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAp16193	XM_013945444.1 Latest version!	Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAp16194	XM_013945445.1 Latest version!	Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAp16195	XM_013945446.1 Latest version!	Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAp16196	XM_013945447.1 Latest version!	Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

MAP3K4 ( XM_013945448.1 ) cDNA ORF clone, Apteryx australis mantelli -> XP_013800902.1 Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OAp16197
Clone ID Related Accession (Same CDS sequence)	XM_013945448.1
Accession Version	XM_013945448.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-08
Organism	Apteryx australis mantelli
Product	mitogen-activated protein kinase kinase kinase 4 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013993955.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apteryx australis mantelli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTTAC CAACTCAGTC TGTGCATAAA GACAATGGGA AAACCATTGA GAATGTGGAA 
GAGCACAGCT ATAAGCAAGG GAAGAAGAAC CGAGCCACCC TAAGGACAAC AGAACGAGAT 
CACAAGAAAA ATGTGCAGTG CTCATTTATG TTAGACTCAG TTGGTGGGTC TTTGCCAAAA 
AAAACAATTC CAGATGTTGA TCTCAATAAG CCTTACCTCA GCCTTGGCTG TAGCAATGCT 
AAGCTTCCAG TCTCTGTGCC CATGCCAATA GCCAGAACTG CACGCCAGAC TTCTAGGACT 
GATTGCCCAG CAGACCGCTT AAAATTCTTT GAAACCCTGC GGCTTTTGTT AAAACTTACC 
TCAGTCTCGA AGAAAAAGGA CAGGGAGCAA AGAGGACAGG AAAACACCTC TTCTGTCTGG 
CTGAACCGAT CCAATGAACT GATCTGGCTG GAGCTGCAAG CTTGGCATGC TGGCCGGAGC 
ATTAATGACC AAGACCTATA TCTCTATGCG GCCCGTCAAG CCATCCCTGA TATTATCAAT 
GAAATCCTCA CCTTCAAAGT AAACTATGGA AGCTTCTCCT GTGTAAAAAA TGGAGCCAGT 
CTCAATGGTA CTACAGGAGA AGGAGTTTGC AAAGCAACAC ATGGAACTAG TGCTTTGGGT 
TACTCAAGTT ACCATGAACA CCTCCATCGC CAGCGGGTCT CATTTGAGCA AGTAAAGCGG 
ATAATGGAGC TGCTAGAGTA TATAGAAGCA CTTTATCCAT CTCTGCAGGC TCTTCAAAAG 
GACTATGAAA AGTATGCTGC AAAGGACTTC CAAGACAGAG TGCAGGCACT CTGTCTGTGG 
TTAAACATTA CAAAAGACTT AAATCAAAAA CTAAGGATTA TGGGAACTGT TTTGGGCATC 
AAAAATCTTT CTGACATTGG CTGGCCAGTG TTTGAAATTC CTTCTCCTGG ACCGTCTAAG 
GACAATGATC CAGAGGATGA GGAAGTTGAT AGGGAAACAG AAGTAAAGGA ATCCTCAGGA 
ATGTGCACAG AGGAGAGTGA TGGTGAAGAA CAAATCTCTG AGGAAAATGT TGAGGAACTT 
AGACAAGCCA TCAAGAACAA TTTTACTAAT AAGCCAAATT TTGACTTTCA GGACCATTTT 
TCAAGGAAGC TTGATAGGCT TGAATCTGAG GATGACTCTG CCTCTTGGGT TATTCCAGAA 
TGCAGTGCTG AGGCAGGCTG CAGCAAACAC TGTTTGACCT CTATTTACAG GCCATTTGTA 
GATAAAGCAC TGAAGCAAAT GGGGTTGAGG AAGCTAATTT TGAGACTGCA CAAGCTAATG 
CATGGTTCAT TGCAAAGGGC CCGTATGGCG TTGGTAAGGA ACGATCACCA GCTGGAGTTT 
TCAGAATTTC CAGATCCCAT GTTGTGTTCA GATTATGTGC AGTTATTAAA TTCCCCGCCT 
TCCTGTGAGA AAAAATGCAG CACTGTATCA TGGGAAGAGC TGAAATCTAT GGATTTACCA 
TCTTTTGAAC CTGCATTCTT GGTCCTTTGC CGAGTCCTGC TCAATGTTAT CCATGAATGT 
CTCAAGCTAA GGTTGGAACA AAGACCTGCT GGGGAACCAT CTCTTTTGAG TATTAAGCAG 
CTAGTAAGAG AGTGTAAAGA GGTCCTGAAG GGCGGCCTGT TAATGAAGCA ATATTATCAG 
TTCATGTTAC GTGAGGTGTT AGATGACTTG CAAAAGATTG ACTGCAACAT TGATTCTTTT 
GAAGAGGACC TGCATAAAAT GTTAATGGTT TACTTTGATT ATATGAGAAG CTGGATCCAA 
ATGCTACAGC AGTTACCTCA AGCATCTCAT AGTTTGAAAA ATCTCTTAGA AGAAGAATGG 
AATTTCACCA AAGAAATAAC TCCTTATATC AGAGGAGGTG AAGCTCAAGC TGGAAAACTA 
TTCTGTGATA TTGCAGGAAT GCTGCTGAAA TCTACAGGAA TATTTTTAGA CTCTGGACTA 
CAGGACAGTT GTGATGAGTT TTGGGCCAGT GCTGATGATA GTACTGCATC AGATGAAATC 
AGGAGGTCTG TTATAGAAAC CAGCCGAGCA CTAAAAGAAC TCTTTCATGA AGCTAGAGAA 
AGGGCATCCA AAGCTCTTGG TTTTGCTAAA ATGCTGAGGA AGGATCTTGA AATAGCAGCA 
GAGTTTGCAT TGTCAGCCCC TGTGCGAGAT TTTCTGAATG CTCTGAAAAC AAAAGAGTAT 
GTGAAGGTGC AAATCCCTGG ACTTGAAAAC TTACAAGTAT TTGTCCCAAA TAGTATTGCA 
GGGGAAAAAT CTGTGATTTT ACAGCTCCTA AATGCAGCTG CAGGAAAGGA TTGCTCGAAG 
GATTCGGATG AGGTTGCATA TGAGGCCTAT TTACTAATGA CCAAACACAG TGACAAAGAC 
CACGAATTAG ACAACAGTTG GAGCGCGTGG GAGGGTCAGC CAGTCAAAGT AGTGCCACAG 
GTGGAAACTG CTGATACACT GCGAAGCATG CAGGTTGATA ACCTCCTCCT TGTAGTCATT 
CAATCTGCCC ATCTTGTGAG TCAGAGAAAA GCTTTCCAAC AGTCTATTGA AGGGCTCATA 
TCTTTGCACC ACGAACAGAC ATCCAGCCAA CCAGTCATTG CCAAAGCTCT GCAACAGCTA 
AAGAGTGATG CATTACAGCT ATGCAATAAG ATAAGCAGTG CCATTGATAG AGTTGATCGC 
ATGTTCACAT CTGAGTTTGA TGCTGAAGTT GATGAATCTG AATCTGCCAC TTTGCAACAA 
TACTACCGGG AAGCCATGAT TCAGGGTTAT AATTTTGGAT TTGAGTACCA TAAAGAAGTT 
GTTCGCCTAA TGTCTGGAGA ATTCAGGCAG AAAATTGGAG ATAAGTATAT CAGCTTTGCT 
AGAAAATGGA TGAATTATGT GCTAACAAAG TGTGAGAGTG GCCGAGGCAC TCGACCCAGG 
TGGGCAACTC AAGGTTTTGA TTTTCTTCAA GCCATTGAGC CAGCATTTAT TTCAGCTCTC 
CCAGAAGATG ACTTCTTGAG TTTACAAGCT CTGATGAATG AATGCATTGG CCATGTTATT 
GGAAAACCTC ATAGTCCTGT TACAGGTTTG TACCTTGCTA TTCATCGAAA CAGTCCCCGT 
CCTGTAAAGG TACCTCGATG CCATAGTGAT CCACCAAATC CTCATCTTAT CATCCCCACT 
CCTGAAGGTT TCAGCACGCG GAGTGTCCCT TGTGATGCAC GGAACCACTG CAGCGCTGCT 
GTCAGTCGCC CCGCTCCCAT TGGTAGCGAC TCAGCTCAAC CCAAACCTGT GAGCAGTGCA 
AATGAGACCA GGGGTTCCAG TGTCCCTGAA AATGATCGAC TGGCTTCAAT AGCAGCTGAG 
TTGCAGTTCA GATCCCTTAG TCGTCACTCC AGTCCCACTG AAGAGCGAGA AGAACCATCT 
TATCCAAAAG GAGATTCAGG TGGATCATCC CGGCGGAGTT GGGAGCTCAG AACTTTAATC 
AACCAGACCA AAGACACTGC TTCCAAGCAA GCGCCAATCG AAGCTGTTCA GAAGTCTGTT 
CAGTTGTTTG AAGAAAGGAG ATACAGAGAG ATGAGGAGGA AGAACATCAT TGGCCAAGTT 
TGTGACACCC CAAAATCTTA TGATAATGTC ATGCATGTAG GTTTGAGGAA AGTGACTTTC 
AAGTGGCAGA GAGGAAACAA AATTGGTGAA GGCCAGTACG GAAAAGTCTA CACCTGTATC 
AGTGTTGACA CTGGTGAACT GATGGCTATG AAAGAAATAA GATTTCAACC CAATGATCAC 
AAAACCATCA AAGAAACAGC AGATGAACTG AAGATTTTTG AAGGCATCAA ACACCCTAAT 
CTTGTTCGTT ACTTTGGTGT GGAGCTCCAT AGGGAAGAAA TGTACATCTT CATGGAGTAC 
TGTGATGAGG GCACCCTGGA GGAGGTATCA AAACTGGGCC TTCAGGAACA TGTCATTAGG 
CTCTACTCAA AGCAAATCAC AACTGCTATC AATGTACTGC ATGAACATGG TATTGTTCAT 
CGAGACATTA AAGGAGCCAA CATCTTTCTT ACCTCATCTG GACTGATTAA ATTAGGAGAC 
TTTGGCTGCT CAGTGAAATT AAAGAATAAC ACCCAGACAA TGCCTGGAGA AGTGAACAGT 
ACTTTGGGGA CTGCAGCATA CATGGCTCCA GAAGTCATTA CTAGAGCTAA AGGAGAAGGG 
CATGGGCGTG CAGCAGACAT CTGGAGCCTG GGCTGTGTTG TAATAGAGAT GGTCACTGGC 
AAGAGGCCGT GGCATGAATA CGAGCACAAC TTCCAGATCA TGTATAGAGT GGGGATGGGT 
CATAAACCTC CTATTCCTGA CAAGGTGAGC CCAGAAGGGA AAGACTTCCT TTGCCACTGT 
CTGGAAAGTG ACCCAAAGAT GAGGTGGACG GCTAGCCAGC TCCTCGACCA TCCCTTTGTC 
AAGGTTTGCA CAGATGAAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013800902.1
CDS	45..4508
Translation	MGLPTQSVHKDNGKTIENVEEHSYKQGKKNRATLRTTERDHKKNVQCSFMLDSVGGSLPKKTIPDVDLNKPYLSLGCSNAKLPVSVPMPIARTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDREQRGQENTSSVWLNRSNELIWLELQAWHAGRSINDQDLYLYAARQAIPDIINEILTFKVNYGSFSCVKNGASLNGTTGEGVCKATHGTSALGYSSYHEHLHRQRVSFEQVKRIMELLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPGPSKDNDPEDEEVDRETEVKESSGMCTEESDGEEQISEENVEELRQAIKNNFTNKPNFDFQDHFSRKLDRLESEDDSASWVIPECSAEAGCSKHCLTSIYRPFVDKALKQMGLRKLILRLHKLMHGSLQRARMALVRNDHQLEFSEFPDPMLCSDYVQLLNSPPSCEKKCSTVSWEELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLREVLDDLQKIDCNIDSFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITPYIRGGEAQAGKLFCDIAGMLLKSTGIFLDSGLQDSCDEFWASADDSTASDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFALSAPVRDFLNALKTKEYVKVQIPGLENLQVFVPNSIAGEKSVILQLLNAAAGKDCSKDSDEVAYEAYLLMTKHSDKDHELDNSWSAWEGQPVKVVPQVETADTLRSMQVDNLLLVVIQSAHLVSQRKAFQQSIEGLISLHHEQTSSQPVIAKALQQLKSDALQLCNKISSAIDRVDRMFTSEFDAEVDESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYLAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFSTRSVPCDARNHCSAAVSRPAPIGSDSAQPKPVSSANETRGSSVPENDRLASIAAELQFRSLSRHSSPTEEREEPSYPKGDSGGSSRRSWELRTLINQTKDTASKQAPIEAVQKSVQLFEERRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSKLGLQEHVIRLYSKQITTAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNTQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYRVGMGHKPPIPDKVSPEGKDFLCHCLESDPKMRWTASQLLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_013945448.1
Organism	Apteryx australis mantelli
Definition	Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013945448.1

ORF Insert Sequence:

ATGGGTTTAC CAACTCAGTC TGTGCATAAA GACAATGGGA AAACCATTGA GAATGTGGAA 
GAGCACAGCT ATAAGCAAGG GAAGAAGAAC CGAGCCACCC TAAGGACAAC AGAACGAGAT 
CACAAGAAAA ATGTGCAGTG CTCATTTATG TTAGACTCAG TTGGTGGGTC TTTGCCAAAA 
AAAACAATTC CAGATGTTGA TCTCAATAAG CCTTACCTCA GCCTTGGCTG TAGCAATGCT 
AAGCTTCCAG TCTCTGTGCC CATGCCAATA GCCAGAACTG CACGCCAGAC TTCTAGGACT 
GATTGCCCAG CAGACCGCTT AAAATTCTTT GAAACCCTGC GGCTTTTGTT AAAACTTACC 
TCAGTCTCGA AGAAAAAGGA CAGGGAGCAA AGAGGACAGG AAAACACCTC TTCTGTCTGG 
CTGAACCGAT CCAATGAACT GATCTGGCTG GAGCTGCAAG CTTGGCATGC TGGCCGGAGC 
ATTAATGACC AAGACCTATA TCTCTATGCG GCCCGTCAAG CCATCCCTGA TATTATCAAT 
GAAATCCTCA CCTTCAAAGT AAACTATGGA AGCTTCTCCT GTGTAAAAAA TGGAGCCAGT 
CTCAATGGTA CTACAGGAGA AGGAGTTTGC AAAGCAACAC ATGGAACTAG TGCTTTGGGT 
TACTCAAGTT ACCATGAACA CCTCCATCGC CAGCGGGTCT CATTTGAGCA AGTAAAGCGG 
ATAATGGAGC TGCTAGAGTA TATAGAAGCA CTTTATCCAT CTCTGCAGGC TCTTCAAAAG 
GACTATGAAA AGTATGCTGC AAAGGACTTC CAAGACAGAG TGCAGGCACT CTGTCTGTGG 
TTAAACATTA CAAAAGACTT AAATCAAAAA CTAAGGATTA TGGGAACTGT TTTGGGCATC 
AAAAATCTTT CTGACATTGG CTGGCCAGTG TTTGAAATTC CTTCTCCTGG ACCGTCTAAG 
GACAATGATC CAGAGGATGA GGAAGTTGAT AGGGAAACAG AAGTAAAGGA ATCCTCAGGA 
ATGTGCACAG AGGAGAGTGA TGGTGAAGAA CAAATCTCTG AGGAAAATGT TGAGGAACTT 
AGACAAGCCA TCAAGAACAA TTTTACTAAT AAGCCAAATT TTGACTTTCA GGACCATTTT 
TCAAGGAAGC TTGATAGGCT TGAATCTGAG GATGACTCTG CCTCTTGGGT TATTCCAGAA 
TGCAGTGCTG AGGCAGGCTG CAGCAAACAC TGTTTGACCT CTATTTACAG GCCATTTGTA 
GATAAAGCAC TGAAGCAAAT GGGGTTGAGG AAGCTAATTT TGAGACTGCA CAAGCTAATG 
CATGGTTCAT TGCAAAGGGC CCGTATGGCG TTGGTAAGGA ACGATCACCA GCTGGAGTTT 
TCAGAATTTC CAGATCCCAT GTTGTGTTCA GATTATGTGC AGTTATTAAA TTCCCCGCCT 
TCCTGTGAGA AAAAATGCAG CACTGTATCA TGGGAAGAGC TGAAATCTAT GGATTTACCA 
TCTTTTGAAC CTGCATTCTT GGTCCTTTGC CGAGTCCTGC TCAATGTTAT CCATGAATGT 
CTCAAGCTAA GGTTGGAACA AAGACCTGCT GGGGAACCAT CTCTTTTGAG TATTAAGCAG 
CTAGTAAGAG AGTGTAAAGA GGTCCTGAAG GGCGGCCTGT TAATGAAGCA ATATTATCAG 
TTCATGTTAC GTGAGGTGTT AGATGACTTG CAAAAGATTG ACTGCAACAT TGATTCTTTT 
GAAGAGGACC TGCATAAAAT GTTAATGGTT TACTTTGATT ATATGAGAAG CTGGATCCAA 
ATGCTACAGC AGTTACCTCA AGCATCTCAT AGTTTGAAAA ATCTCTTAGA AGAAGAATGG 
AATTTCACCA AAGAAATAAC TCCTTATATC AGAGGAGGTG AAGCTCAAGC TGGAAAACTA 
TTCTGTGATA TTGCAGGAAT GCTGCTGAAA TCTACAGGAA TATTTTTAGA CTCTGGACTA 
CAGGACAGTT GTGATGAGTT TTGGGCCAGT GCTGATGATA GTACTGCATC AGATGAAATC 
AGGAGGTCTG TTATAGAAAC CAGCCGAGCA CTAAAAGAAC TCTTTCATGA AGCTAGAGAA 
AGGGCATCCA AAGCTCTTGG TTTTGCTAAA ATGCTGAGGA AGGATCTTGA AATAGCAGCA 
GAGTTTGCAT TGTCAGCCCC TGTGCGAGAT TTTCTGAATG CTCTGAAAAC AAAAGAGTAT 
GTGAAGGTGC AAATCCCTGG ACTTGAAAAC TTACAAGTAT TTGTCCCAAA TAGTATTGCA 
GGGGAAAAAT CTGTGATTTT ACAGCTCCTA AATGCAGCTG CAGGAAAGGA TTGCTCGAAG 
GATTCGGATG AGGTTGCATA TGAGGCCTAT TTACTAATGA CCAAACACAG TGACAAAGAC 
CACGAATTAG ACAACAGTTG GAGCGCGTGG GAGGGTCAGC CAGTCAAAGT AGTGCCACAG 
GTGGAAACTG CTGATACACT GCGAAGCATG CAGGTTGATA ACCTCCTCCT TGTAGTCATT 
CAATCTGCCC ATCTTGTGAG TCAGAGAAAA GCTTTCCAAC AGTCTATTGA AGGGCTCATA 
TCTTTGCACC ACGAACAGAC ATCCAGCCAA CCAGTCATTG CCAAAGCTCT GCAACAGCTA 
AAGAGTGATG CATTACAGCT ATGCAATAAG ATAAGCAGTG CCATTGATAG AGTTGATCGC 
ATGTTCACAT CTGAGTTTGA TGCTGAAGTT GATGAATCTG AATCTGCCAC TTTGCAACAA 
TACTACCGGG AAGCCATGAT TCAGGGTTAT AATTTTGGAT TTGAGTACCA TAAAGAAGTT 
GTTCGCCTAA TGTCTGGAGA ATTCAGGCAG AAAATTGGAG ATAAGTATAT CAGCTTTGCT 
AGAAAATGGA TGAATTATGT GCTAACAAAG TGTGAGAGTG GCCGAGGCAC TCGACCCAGG 
TGGGCAACTC AAGGTTTTGA TTTTCTTCAA GCCATTGAGC CAGCATTTAT TTCAGCTCTC 
CCAGAAGATG ACTTCTTGAG TTTACAAGCT CTGATGAATG AATGCATTGG CCATGTTATT 
GGAAAACCTC ATAGTCCTGT TACAGGTTTG TACCTTGCTA TTCATCGAAA CAGTCCCCGT 
CCTGTAAAGG TACCTCGATG CCATAGTGAT CCACCAAATC CTCATCTTAT CATCCCCACT 
CCTGAAGGTT TCAGCACGCG GAGTGTCCCT TGTGATGCAC GGAACCACTG CAGCGCTGCT 
GTCAGTCGCC CCGCTCCCAT TGGTAGCGAC TCAGCTCAAC CCAAACCTGT GAGCAGTGCA 
AATGAGACCA GGGGTTCCAG TGTCCCTGAA AATGATCGAC TGGCTTCAAT AGCAGCTGAG 
TTGCAGTTCA GATCCCTTAG TCGTCACTCC AGTCCCACTG AAGAGCGAGA AGAACCATCT 
TATCCAAAAG GAGATTCAGG TGGATCATCC CGGCGGAGTT GGGAGCTCAG AACTTTAATC 
AACCAGACCA AAGACACTGC TTCCAAGCAA GCGCCAATCG AAGCTGTTCA GAAGTCTGTT 
CAGTTGTTTG AAGAAAGGAG ATACAGAGAG ATGAGGAGGA AGAACATCAT TGGCCAAGTT 
TGTGACACCC CAAAATCTTA TGATAATGTC ATGCATGTAG GTTTGAGGAA AGTGACTTTC 
AAGTGGCAGA GAGGAAACAA AATTGGTGAA GGCCAGTACG GAAAAGTCTA CACCTGTATC 
AGTGTTGACA CTGGTGAACT GATGGCTATG AAAGAAATAA GATTTCAACC CAATGATCAC 
AAAACCATCA AAGAAACAGC AGATGAACTG AAGATTTTTG AAGGCATCAA ACACCCTAAT 
CTTGTTCGTT ACTTTGGTGT GGAGCTCCAT AGGGAAGAAA TGTACATCTT CATGGAGTAC 
TGTGATGAGG GCACCCTGGA GGAGGTATCA AAACTGGGCC TTCAGGAACA TGTCATTAGG 
CTCTACTCAA AGCAAATCAC AACTGCTATC AATGTACTGC ATGAACATGG TATTGTTCAT 
CGAGACATTA AAGGAGCCAA CATCTTTCTT ACCTCATCTG GACTGATTAA ATTAGGAGAC 
TTTGGCTGCT CAGTGAAATT AAAGAATAAC ACCCAGACAA TGCCTGGAGA AGTGAACAGT 
ACTTTGGGGA CTGCAGCATA CATGGCTCCA GAAGTCATTA CTAGAGCTAA AGGAGAAGGG 
CATGGGCGTG CAGCAGACAT CTGGAGCCTG GGCTGTGTTG TAATAGAGAT GGTCACTGGC 
AAGAGGCCGT GGCATGAATA CGAGCACAAC TTCCAGATCA TGTATAGAGT GGGGATGGGT 
CATAAACCTC CTATTCCTGA CAAGGTGAGC CCAGAAGGGA AAGACTTCCT TTGCCACTGT 
CTGGAAAGTG ACCCAAAGAT GAGGTGGACG GCTAGCCAGC TCCTCGACCA TCCCTTTGTC 
AAGGTTTGCA CAGATGAAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_013945449.1 ) cDNA ORF clone, Apteryx australis mantelli -> XP_013800903.1 Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OAp16198
Clone ID Related Accession (Same CDS sequence)	XM_013945449.1
Accession Version	XM_013945449.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-08
Organism	Apteryx australis mantelli
Product	mitogen-activated protein kinase kinase kinase 4 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013993955.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apteryx australis mantelli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGACG TCGCCGCCGT CGCGCTGGTC TCTGCTGCCG CCGCCATGGA GGAGCCGCCG 
CCGCCGGGGA GGACAGAGTG CAAATCAGAA GATGCTACTT GCAAGATCTC TGAATCTGAA 
CCTGAGGAAT TATCAGATGA AGCAAGTGCT GACACTTTCT ATGGAACTTC TCCTCCTAGT 
ACGCCCCGAC AGATGAAACG TATGTCAACA AAGCATCAGA AAAATAATGT TGGGAGACCT 
GCTAGCCGTT CCACTTTGAA AGAAAAAATG GGTTTACCAA CTCAGTCTGT GCATAAAGAC 
AATGGGAAAA CCATTGAGAA TGTGGAAGAG CACAGCTATA AGCAAGGGAA GAAGAACCGA 
GCCACCCTAA GGACAACAGA ACGAGATCAC AAGAAAAATG TGCAGTGCTC ATTTATGTTA 
GACTCAGTTG GTGGGTCTTT GCCAAAAAAA ACAATTCCAG ATGTTGATCT CAATAAGCCT 
TACCTCAGCC TTGGCTGTAG CAATGCTAAG CTTCCAGTCT CTGTGCCCAT GCCAATAGCC 
AGAACTGCAC GCCAGACTTC TAGGACTGAT TGCCCAGCAG ACCGCTTAAA ATTCTTTGAA 
ACCCTGCGGC TTTTGTTAAA ACTTACCTCA GTCTCGAAGA AAAAGGACAG GGAGCAAAGA 
GGACAGGAAA ACACCTCTTC TGTCTGGCTG AACCGATCCA ATGAACTGAT CTGGCTGGAG 
CTGCAAGCTT GGCATGCTGG CCGGAGCATT AATGACCAAG ACCTATATCT CTATGCGGCC 
CGTCAAGCCA TCCCTGATAT TATCAATGAA ATCCTCACCT TCAAAGTAAA CTATGGAAGC 
TTCTCCTGTG TAAAAAATGG AGCCAGTCTC AATGGTACTA CAGGAGAAGG AGTTTGCAAA 
GCAACACATG GAACTAGTGC TTTGGGTTAC TCAAGTTACC ATGAACACCT CCATCGCCAG 
CGGGTCTCAT TTGAGCAAGT AAAGCGGATA ATGGAGCTGC TAGAGTATAT AGAAGCACTT 
TATCCATCTC TGCAGGCTCT TCAAAAGGAC TATGAAAAGT ATGCTGCAAA GGACTTCCAA 
GACAGAGTGC AGGCACTCTG TCTGTGGTTA AACATTACAA AAGACTTAAA TCAAAAACTA 
AGGATTATGG GAACTGTTTT GGGCATCAAA AATCTTTCTG ACATTGGCTG GCCAGTGTTT 
GAAATTCCTT CTCCTGGACC GTCTAAGGAC AATGATCCAG AGGATGAGGA AGTTGATAGG 
GAAACAGAAG TAAAGGAATC CTCAGGAATG TGCACAGAGG AGAGTGATGG TGAAGAACAA 
ATCTCTGAGG AAAATGTTGA GGAACTTAGA CAAGCCATCA AGAACAATTT TACTAATAAG 
CCAAATTTTG ACTTTCAGGA CCATTTTTCA AGGAAGCTTG ATAGGCTTGA ATCTGAGGAT 
GACTCTGCCT CTTGGGTTAT TCCAGAATGC AGTGCTGAGG CAGGCTGCAG CAAACACTGT 
TTGACCTCTA TTTACAGGCC ATTTGTAGAT AAAGCACTGA AGCAAATGGG GTTGAGGAAG 
CTAATTTTGA GACTGCACAA GCTAATGCAT GGTTCATTGC AAAGGGCCCG TATGGCGTTG 
GTAAGGAACG ATCACCAGCT GGAGTTTTCA GAATTTCCAG ATCCCATGTT GTGTTCAGAT 
TATGTGCAGT TATTAAATTC CCCGCCTTCC TGTGAGAAAA AATGCAGCAC TGTATCATGG 
GAAGAGCTGA AATCTATGGA TTTACCATCT TTTGAACCTG CATTCTTGGT CCTTTGCCGA 
GTCCTGCTCA ATGTTATCCA TGAATGTCTC AAGCTAAGGT TGGAACAAAG ACCTGCTGGG 
GAACCATCTC TTTTGAGTAT TAAGCAGCTA GTAAGAGAGT GTAAAGAGGT CCTGAAGGGC 
GGCCTGTTAA TGAAGCAATA TTATCAGTTC ATGTTACGTG AGGTGTTAGA TGACTTGCAA 
AAGATTGACT GCAACATTGA TTCTTTTGAA GAGGACCTGC ATAAAATGTT AATGGTTTAC 
TTTGATTATA TGAGAAGCTG GATCCAAATG CTACAGCAGT TACCTCAAGC ATCTCATAGT 
TTGAAAAATC TCTTAGAAGA AGAATGGAAT TTCACCAAAG AAATAACTCC TTATATCAGA 
GGAGGTGAAG CTCAAGCTGG AAAACTATTC TGTGATATTG CAGGAATGCT GCTGAAATCT 
ACAGGAATAT TTTTAGACTC TGGACTACAG GACAGTTGTG ATGAGTTTTG GGCCAGTGCT 
GATGATAGTA CTGCATCAGA TGAAATCAGG AGGTCTGTTA TAGAAACCAG CCGAGCACTA 
AAAGAACTCT TTCATGAAGC TAGAGAAAGG GCATCCAAAG CTCTTGGTTT TGCTAAAATG 
CTGAGGAAGG ATCTTGAAAT AGCAGCAGAG TTTGCATTGT CAGCCCCTGT GCGAGATTTT 
CTGAATGCTC TGAAAACAAA AGAGTATGTG AAGGTGCAAA TCCCTGGACT TGAAAACTTA 
CAAGTATTTG TCCCAAATAG TATTGCAGGG GAAAAATCTG TGATTTTACA GCTCCTAAAT 
GCAGCTGCAG GAAAGGATTG CTCGAAGGAT TCGGATGAGG TTGCATATGA GGCCTATTTA 
CTAATGACCA AACACAGTGA CAAAGACCAC GAATTAGACA ACAGTTGGAG CGCGTGGGAG 
GGTCAGCCAG TCAAAGTAGT GCCACAGGTG GAAACTGCTG ATACACTGCG AAGCATGCAG 
GTTGATAACC TCCTCCTTGT AGTCATTCAA TCTGCCCATC TTGTGAGTCA GAGAAAAGCT 
TTCCAACAGT CTATTGAAGG GCTCATATCT TTGCACCACG AACAGACATC CAGCCAACCA 
GTCATTGCCA AAGCTCTGCA ACAGCTAAAG AGTGATGCAT TACAGCTATG CAATAAGATA 
AGCAGTGCCA TTGATAGAGT TGATCGCATG TTCACATCTG AGTTTGATGC TGAAGTTGAT 
GAATCTGAAT CTGCCACTTT GCAACAATAC TACCGGGAAG CCATGATTCA GGGTTATAAT 
TTTGGATTTG AGTACCATAA AGAAGTTGTT CGCCTAATGT CTGGAGAATT CAGGCAGAAA 
ATTGGAGATA AGTATATCAG CTTTGCTAGA AAATGGATGA ATTATGTGCT AACAAAGTGT 
GAGAGTGGCC GAGGCACTCG ACCCAGGTGG GCAACTCAAG GTTTTGATTT TCTTCAAGCC 
ATTGAGCCAG CATTTATTTC AGCTCTCCCA GAAGATGACT TCTTGAGTTT ACAAGCTCTG 
ATGAATGAAT GCATTGGCCA TGTTATTGGA AAACCTCATA GTCCTGTTAC AGCTATTCAT 
CGAAACAGTC CCCGTCCTGT AAAGGTACCT CGATGCCATA GTGATCCACC AAATCCTCAT 
CTTATCATCC CCACTCCTGA AGGTTTCAGT TTTTCTTCTT GCCACAGCAC GCGGAGTGTC 
CCTTGTGATG CACGGAACCA CTGCAGCGCT GCTGTCAGTC GCCCCGCTCC CATTGGTAGC 
GACTCAGCTC AACCCAAACC TGTGAGCAGT GCAAATGAGA CCAGGGGTTC CAGTGTCCCT 
GAAAATGATC GACTGGCTTC AATAGCAGCT GAGTTGCAGT TCAGATCCCT TAGTCGTCAC 
TCCAGTCCCA CTGAAGAGCG AGAAGGTGAA GGCCAGTACG GAAAAGTCTA CACCTGTATC 
AGTGTTGACA CTGGTGAACT GATGGCTATG AAAGAAATAA GATTTCAACC CAATGATCAC 
AAAACCATCA AAGAAACAGC AGATGAACTG AAGATTTTTG AAGGCATCAA ACACCCTAAT 
CTTGTTCGTT ACTTTGGTGT GGAGCTCCAT AGGGAAGAAA TGTACATCTT CATGGAGTAC 
TGTGATGAGG GCACCCTGGA GGAGGTATCA AAACTGGGCC TTCAGGAACA TGTCATTAGG 
CTCTACTCAA AGCAAATCAC AACTGCTATC AATGTACTGC ATGAACATGG TATTGTTCAT 
CGAGACATTA AAGGAGCCAA CATCTTTCTT ACCTCATCTG GACTGATTAA ATTAGGAGAC 
TTTGGCTGCT CAGTGAAATT AAAGAATAAC ACCCAGACAA TGCCTGGAGA AGTGAACAGT 
ACTTTGGGGA CTGCAGCATA CATGGCTCCA GAAGTCATTA CTAGAGCTAA AGGAGAAGGG 
CATGGGCGTG CAGCAGACAT CTGGAGCCTG GGCTGTGTTG TAATAGAGAT GGTCACTGGC 
AAGAGGCCGT GGCATGAATA CGAGCACAAC TTCCAGATCA TGTATAGAGT GGGGATGGGT 
CATAAACCTC CTATTCCTGA CAAGGTGAGC CCAGAAGGGA AAGACTTCCT TTGCCACTGT 
CTGGAAAGTG ACCCAAAGAT GAGGTGGACG GCTAGCCAGC TCCTCGACCA TCCCTTTGTC 
AAGGTTTGCA CAGATGAAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013800903.1
CDS	140..4603
Translation	MRDVAAVALVSAAAAMEEPPPPGRTECKSEDATCKISESEPEELSDEASADTFYGTSPPSTPRQMKRMSTKHQKNNVGRPASRSTLKEKMGLPTQSVHKDNGKTIENVEEHSYKQGKKNRATLRTTERDHKKNVQCSFMLDSVGGSLPKKTIPDVDLNKPYLSLGCSNAKLPVSVPMPIARTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDREQRGQENTSSVWLNRSNELIWLELQAWHAGRSINDQDLYLYAARQAIPDIINEILTFKVNYGSFSCVKNGASLNGTTGEGVCKATHGTSALGYSSYHEHLHRQRVSFEQVKRIMELLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPGPSKDNDPEDEEVDRETEVKESSGMCTEESDGEEQISEENVEELRQAIKNNFTNKPNFDFQDHFSRKLDRLESEDDSASWVIPECSAEAGCSKHCLTSIYRPFVDKALKQMGLRKLILRLHKLMHGSLQRARMALVRNDHQLEFSEFPDPMLCSDYVQLLNSPPSCEKKCSTVSWEELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLREVLDDLQKIDCNIDSFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITPYIRGGEAQAGKLFCDIAGMLLKSTGIFLDSGLQDSCDEFWASADDSTASDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFALSAPVRDFLNALKTKEYVKVQIPGLENLQVFVPNSIAGEKSVILQLLNAAAGKDCSKDSDEVAYEAYLLMTKHSDKDHELDNSWSAWEGQPVKVVPQVETADTLRSMQVDNLLLVVIQSAHLVSQRKAFQQSIEGLISLHHEQTSSQPVIAKALQQLKSDALQLCNKISSAIDRVDRMFTSEFDAEVDESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFSFSSCHSTRSVPCDARNHCSAAVSRPAPIGSDSAQPKPVSSANETRGSSVPENDRLASIAAELQFRSLSRHSSPTEEREGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSKLGLQEHVIRLYSKQITTAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNTQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYRVGMGHKPPIPDKVSPEGKDFLCHCLESDPKMRWTASQLLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_013945449.1
Organism	Apteryx australis mantelli
Definition	Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013945449.1

ORF Insert Sequence:

ATGAGAGACG TCGCCGCCGT CGCGCTGGTC TCTGCTGCCG CCGCCATGGA GGAGCCGCCG 
CCGCCGGGGA GGACAGAGTG CAAATCAGAA GATGCTACTT GCAAGATCTC TGAATCTGAA 
CCTGAGGAAT TATCAGATGA AGCAAGTGCT GACACTTTCT ATGGAACTTC TCCTCCTAGT 
ACGCCCCGAC AGATGAAACG TATGTCAACA AAGCATCAGA AAAATAATGT TGGGAGACCT 
GCTAGCCGTT CCACTTTGAA AGAAAAAATG GGTTTACCAA CTCAGTCTGT GCATAAAGAC 
AATGGGAAAA CCATTGAGAA TGTGGAAGAG CACAGCTATA AGCAAGGGAA GAAGAACCGA 
GCCACCCTAA GGACAACAGA ACGAGATCAC AAGAAAAATG TGCAGTGCTC ATTTATGTTA 
GACTCAGTTG GTGGGTCTTT GCCAAAAAAA ACAATTCCAG ATGTTGATCT CAATAAGCCT 
TACCTCAGCC TTGGCTGTAG CAATGCTAAG CTTCCAGTCT CTGTGCCCAT GCCAATAGCC 
AGAACTGCAC GCCAGACTTC TAGGACTGAT TGCCCAGCAG ACCGCTTAAA ATTCTTTGAA 
ACCCTGCGGC TTTTGTTAAA ACTTACCTCA GTCTCGAAGA AAAAGGACAG GGAGCAAAGA 
GGACAGGAAA ACACCTCTTC TGTCTGGCTG AACCGATCCA ATGAACTGAT CTGGCTGGAG 
CTGCAAGCTT GGCATGCTGG CCGGAGCATT AATGACCAAG ACCTATATCT CTATGCGGCC 
CGTCAAGCCA TCCCTGATAT TATCAATGAA ATCCTCACCT TCAAAGTAAA CTATGGAAGC 
TTCTCCTGTG TAAAAAATGG AGCCAGTCTC AATGGTACTA CAGGAGAAGG AGTTTGCAAA 
GCAACACATG GAACTAGTGC TTTGGGTTAC TCAAGTTACC ATGAACACCT CCATCGCCAG 
CGGGTCTCAT TTGAGCAAGT AAAGCGGATA ATGGAGCTGC TAGAGTATAT AGAAGCACTT 
TATCCATCTC TGCAGGCTCT TCAAAAGGAC TATGAAAAGT ATGCTGCAAA GGACTTCCAA 
GACAGAGTGC AGGCACTCTG TCTGTGGTTA AACATTACAA AAGACTTAAA TCAAAAACTA 
AGGATTATGG GAACTGTTTT GGGCATCAAA AATCTTTCTG ACATTGGCTG GCCAGTGTTT 
GAAATTCCTT CTCCTGGACC GTCTAAGGAC AATGATCCAG AGGATGAGGA AGTTGATAGG 
GAAACAGAAG TAAAGGAATC CTCAGGAATG TGCACAGAGG AGAGTGATGG TGAAGAACAA 
ATCTCTGAGG AAAATGTTGA GGAACTTAGA CAAGCCATCA AGAACAATTT TACTAATAAG 
CCAAATTTTG ACTTTCAGGA CCATTTTTCA AGGAAGCTTG ATAGGCTTGA ATCTGAGGAT 
GACTCTGCCT CTTGGGTTAT TCCAGAATGC AGTGCTGAGG CAGGCTGCAG CAAACACTGT 
TTGACCTCTA TTTACAGGCC ATTTGTAGAT AAAGCACTGA AGCAAATGGG GTTGAGGAAG 
CTAATTTTGA GACTGCACAA GCTAATGCAT GGTTCATTGC AAAGGGCCCG TATGGCGTTG 
GTAAGGAACG ATCACCAGCT GGAGTTTTCA GAATTTCCAG ATCCCATGTT GTGTTCAGAT 
TATGTGCAGT TATTAAATTC CCCGCCTTCC TGTGAGAAAA AATGCAGCAC TGTATCATGG 
GAAGAGCTGA AATCTATGGA TTTACCATCT TTTGAACCTG CATTCTTGGT CCTTTGCCGA 
GTCCTGCTCA ATGTTATCCA TGAATGTCTC AAGCTAAGGT TGGAACAAAG ACCTGCTGGG 
GAACCATCTC TTTTGAGTAT TAAGCAGCTA GTAAGAGAGT GTAAAGAGGT CCTGAAGGGC 
GGCCTGTTAA TGAAGCAATA TTATCAGTTC ATGTTACGTG AGGTGTTAGA TGACTTGCAA 
AAGATTGACT GCAACATTGA TTCTTTTGAA GAGGACCTGC ATAAAATGTT AATGGTTTAC 
TTTGATTATA TGAGAAGCTG GATCCAAATG CTACAGCAGT TACCTCAAGC ATCTCATAGT 
TTGAAAAATC TCTTAGAAGA AGAATGGAAT TTCACCAAAG AAATAACTCC TTATATCAGA 
GGAGGTGAAG CTCAAGCTGG AAAACTATTC TGTGATATTG CAGGAATGCT GCTGAAATCT 
ACAGGAATAT TTTTAGACTC TGGACTACAG GACAGTTGTG ATGAGTTTTG GGCCAGTGCT 
GATGATAGTA CTGCATCAGA TGAAATCAGG AGGTCTGTTA TAGAAACCAG CCGAGCACTA 
AAAGAACTCT TTCATGAAGC TAGAGAAAGG GCATCCAAAG CTCTTGGTTT TGCTAAAATG 
CTGAGGAAGG ATCTTGAAAT AGCAGCAGAG TTTGCATTGT CAGCCCCTGT GCGAGATTTT 
CTGAATGCTC TGAAAACAAA AGAGTATGTG AAGGTGCAAA TCCCTGGACT TGAAAACTTA 
CAAGTATTTG TCCCAAATAG TATTGCAGGG GAAAAATCTG TGATTTTACA GCTCCTAAAT 
GCAGCTGCAG GAAAGGATTG CTCGAAGGAT TCGGATGAGG TTGCATATGA GGCCTATTTA 
CTAATGACCA AACACAGTGA CAAAGACCAC GAATTAGACA ACAGTTGGAG CGCGTGGGAG 
GGTCAGCCAG TCAAAGTAGT GCCACAGGTG GAAACTGCTG ATACACTGCG AAGCATGCAG 
GTTGATAACC TCCTCCTTGT AGTCATTCAA TCTGCCCATC TTGTGAGTCA GAGAAAAGCT 
TTCCAACAGT CTATTGAAGG GCTCATATCT TTGCACCACG AACAGACATC CAGCCAACCA 
GTCATTGCCA AAGCTCTGCA ACAGCTAAAG AGTGATGCAT TACAGCTATG CAATAAGATA 
AGCAGTGCCA TTGATAGAGT TGATCGCATG TTCACATCTG AGTTTGATGC TGAAGTTGAT 
GAATCTGAAT CTGCCACTTT GCAACAATAC TACCGGGAAG CCATGATTCA GGGTTATAAT 
TTTGGATTTG AGTACCATAA AGAAGTTGTT CGCCTAATGT CTGGAGAATT CAGGCAGAAA 
ATTGGAGATA AGTATATCAG CTTTGCTAGA AAATGGATGA ATTATGTGCT AACAAAGTGT 
GAGAGTGGCC GAGGCACTCG ACCCAGGTGG GCAACTCAAG GTTTTGATTT TCTTCAAGCC 
ATTGAGCCAG CATTTATTTC AGCTCTCCCA GAAGATGACT TCTTGAGTTT ACAAGCTCTG 
ATGAATGAAT GCATTGGCCA TGTTATTGGA AAACCTCATA GTCCTGTTAC AGCTATTCAT 
CGAAACAGTC CCCGTCCTGT AAAGGTACCT CGATGCCATA GTGATCCACC AAATCCTCAT 
CTTATCATCC CCACTCCTGA AGGTTTCAGT TTTTCTTCTT GCCACAGCAC GCGGAGTGTC 
CCTTGTGATG CACGGAACCA CTGCAGCGCT GCTGTCAGTC GCCCCGCTCC CATTGGTAGC 
GACTCAGCTC AACCCAAACC TGTGAGCAGT GCAAATGAGA CCAGGGGTTC CAGTGTCCCT 
GAAAATGATC GACTGGCTTC AATAGCAGCT GAGTTGCAGT TCAGATCCCT TAGTCGTCAC 
TCCAGTCCCA CTGAAGAGCG AGAAGGTGAA GGCCAGTACG GAAAAGTCTA CACCTGTATC 
AGTGTTGACA CTGGTGAACT GATGGCTATG AAAGAAATAA GATTTCAACC CAATGATCAC 
AAAACCATCA AAGAAACAGC AGATGAACTG AAGATTTTTG AAGGCATCAA ACACCCTAAT 
CTTGTTCGTT ACTTTGGTGT GGAGCTCCAT AGGGAAGAAA TGTACATCTT CATGGAGTAC 
TGTGATGAGG GCACCCTGGA GGAGGTATCA AAACTGGGCC TTCAGGAACA TGTCATTAGG 
CTCTACTCAA AGCAAATCAC AACTGCTATC AATGTACTGC ATGAACATGG TATTGTTCAT 
CGAGACATTA AAGGAGCCAA CATCTTTCTT ACCTCATCTG GACTGATTAA ATTAGGAGAC 
TTTGGCTGCT CAGTGAAATT AAAGAATAAC ACCCAGACAA TGCCTGGAGA AGTGAACAGT 
ACTTTGGGGA CTGCAGCATA CATGGCTCCA GAAGTCATTA CTAGAGCTAA AGGAGAAGGG 
CATGGGCGTG CAGCAGACAT CTGGAGCCTG GGCTGTGTTG TAATAGAGAT GGTCACTGGC 
AAGAGGCCGT GGCATGAATA CGAGCACAAC TTCCAGATCA TGTATAGAGT GGGGATGGGT 
CATAAACCTC CTATTCCTGA CAAGGTGAGC CCAGAAGGGA AAGACTTCCT TTGCCACTGT 
CTGGAAAGTG ACCCAAAGAT GAGGTGGACG GCTAGCCAGC TCCTCGACCA TCCCTTTGTC 
AAGGTTTGCA CAGATGAAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_013945450.1 ) cDNA ORF clone, Apteryx australis mantelli -> XP_013800904.1 Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X9, mRNA.

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CloneID	OAp16199
Clone ID Related Accession (Same CDS sequence)	XM_013945450.1
Accession Version	XM_013945450.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-08
Organism	Apteryx australis mantelli
Product	mitogen-activated protein kinase kinase kinase 4 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013993955.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apteryx australis mantelli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGACG TCGCCGCCGT CGCGCTGGTC TCTGCTGCCG CCGCCATGGA GGAGCCGCCG 
CCGCCGGGGA GGACAGAGTG CAAATCAGAA GATGCTACTT GCAAGATCTC TGAATCTGAA 
CCTGAGGAAT TATCAGATGA AGCAAGTGCT GACACTTTCT ATGGAACTTC TCCTCCTAGT 
ACGCCCCGAC AGATGAAACG TATGTCAACA AAGCATCAGA AAAATAATGT TGGGAGACCT 
GCTAGCCGTT CCACTTTGAA AGAAAAAATG GGTTTACCAA CTCAGTCTGT GCATAAAGAC 
AATGGGAAAA CCATTGAGAA TGTGGAAGAG CACAGCTATA AGCAAGGGAA GAAGAACCGA 
GCCACCCTAA GGACAACAGA ACGAGATCAC AAGAAAAATG TGCAGTGCTC ATTTATGTTA 
GACTCAGTTG GTGGGTCTTT GCCAAAAAAA ACAATTCCAG ATGTTGATCT CAATAAGCCT 
TACCTCAGCC TTGGCTGTAG CAATGCTAAG CTTCCAGTCT CTGTGCCCAT GCCAATAGCC 
AGAACTGCAC GCCAGACTTC TAGGACTGAT TGCCCAGCAG ACCGCTTAAA ATTCTTTGAA 
ACCCTGCGGC TTTTGTTAAA ACTTACCTCA GTCTCGAAGA AAAAGGACAG GGAGCAAAGA 
GGACAGGAAA ACACCTCTTC TGTCTGGCTG AACCGATCCA ATGAACTGAT CTGGCTGGAG 
CTGCAAGCTT GGCATGCTGG CCGGAGCATT AATGACCAAG ACCTATATCT CTATGCGGCC 
CGTCAAGCCA TCCCTGATAT TATCAATGAA ATCCTCACCT TCAAAGTAAA CTATGGAAGC 
TTCTCCTGTG TAAAAAATGG AGCCAGTCTC AATGGTACTA CAGGAGAAGG AGTTTGCAAA 
GCAACACATG GAACTAGTGC TTTGGGTTAC TCAAGTTACC ATGAACACCT CCATCGCCAG 
CGGGTCTCAT TTGAGCAAGT AAAGCGGATA ATGGAGCTGC TAGAGTATAT AGAAGCACTT 
TATCCATCTC TGCAGGCTCT TCAAAAGGAC TATGAAAAGT ATGCTGCAAA GGACTTCCAA 
GACAGAGTGC AGGCACTCTG TCTGTGGTTA AACATTACAA AAGACTTAAA TCAAAAACTA 
AGGATTATGG GAACTGTTTT GGGCATCAAA AATCTTTCTG ACATTGGCTG GCCAGTGTTT 
GAAATTCCTT CTCCTGGACC GTCTAAGGAC AATGATCCAG AGGATGAGGA AGTTGATAGG 
GAAACAGAAG TAAAGGAATC CTCAGGAATG TGCACAGAGG AGAGTGATGG TGAAGAACAA 
ATCTCTGAGG AAAATGTTGA GGAACTTAGA CAAGCCATCA AGAACAATTT TACTAATAAG 
CCAAATTTTG ACTTTCAGGA CCATTTTTCA AGGAAGCTTG ATAGGCTTGA ATCTGAGGAT 
GACTCTGCCT CTTGGGTTAT TCCAGAATGC AGTGCTGAGG CAGGCTGCAG CAAACACTGT 
TTGACCTCTA TTTACAGGCC ATTTGTAGAT AAAGCACTGA AGCAAATGGG GTTGAGGAAG 
CTAATTTTGA GACTGCACAA GCTAATGCAT GGTTCATTGC AAAGGGCCCG TATGGCGTTG 
GTAAGGAACG ATCACCAGCT GGAGTTTTCA GAATTTCCAG ATCCCATGTT GTGTTCAGAT 
TATGTGCAGT TATTAAATTC CCCGCCTTCC TGTGAGAAAA AATGCAGCAC TGTATCATGG 
GAAGAGCTGA AATCTATGGA TTTACCATCT TTTGAACCTG CATTCTTGGT CCTTTGCCGA 
GTCCTGCTCA ATGTTATCCA TGAATGTCTC AAGCTAAGGT TGGAACAAAG ACCTGCTGGG 
GAACCATCTC TTTTGAGTAT TAAGCAGCTA GTAAGAGAGT GTAAAGAGGT CCTGAAGGGC 
GGCCTGTTAA TGAAGCAATA TTATCAGTTC ATGTTACGTG AGGTGTTAGA TGACTTGCAA 
AAGATTGACT GCAACATTGA TTCTTTTGAA GAGGACCTGC ATAAAATGTT AATGGTTTAC 
TTTGATTATA TGAGAAGCTG GATCCAAATG CTACAGCAGT TACCTCAAGC ATCTCATAGT 
TTGAAAAATC TCTTAGAAGA AGAATGGAAT TTCACCAAAG AAATAACTCC TTATATCAGA 
GGAGGTGAAG CTCAAGCTGG AAAACTATTC TGTGATATTG CAGGAATGCT GCTGAAATCT 
ACAGGAATAT TTTTAGACTC TGGACTACAG GACAGTTGTG ATGAGTTTTG GGCCAGTGCT 
GATGATAGTA CTGCATCAGA TGAAATCAGG AGGTCTGTTA TAGAAACCAG CCGAGCACTA 
AAAGAACTCT TTCATGAAGC TAGAGAAAGG GCATCCAAAG CTCTTGGTTT TGCTAAAATG 
CTGAGGAAGG ATCTTGAAAT AGCAGCAGAG TTTGCATTGT CAGCCCCTGT GCGAGATTTT 
CTGAATGCTC TGAAAACAAA AGAGTATGTG AAGGTGCAAA TCCCTGGACT TGAAAACTTA 
CAAGTATTTG TCCCAAATAG TATTGCAGGG GAAAAATCTG TGATTTTACA GCTCCTAAAT 
GCAGCTGCAG GAAAGGATTG CTCGAAGGAT TCGGATGAGG TTGCATATGA GGCCTATTTA 
CTAATGACCA AACACAGTGA CAAAGACCAC GAATTAGACA ACAGTTGGAG CGCGTGGGAG 
GGTCAGCCAG TCAAAGTAGT GCCACAGGTG GAAACTGCTG ATACACTGCG AAGCATGCAG 
GTTGATAACC TCCTCCTTGT AGTCATTCAA TCTGCCCATC TTGTGAGTCA GAGAAAAGCT 
TTCCAACAGT CTATTGAAGG GCTCATATCT TTGCACCACG AACAGACATC CAGCCAACCA 
GTCATTGCCA AAGCTCTGCA ACAGCTAAAG AGTGATGCAT TACAGCTATG CAATAAGATA 
AGCAGTGCCA TTGATAGAGT TGATCGCATG TTCACATCTG AGTTTGATGC TGAAGTTGAT 
GAATCTGAAT CTGCCACTTT GCAACAATAC TACCGGGAAG CCATGATTCA GGGTTATAAT 
TTTGGATTTG AGTACCATAA AGAAGTTGTT CGCCTAATGT CTGGAGAATT CAGGCAGAAA 
ATTGGAGATA AGTATATCAG CTTTGCTAGA AAATGGATGA ATTATGTGCT AACAAAGTGT 
GAGAGTGGCC GAGGCACTCG ACCCAGGTGG GCAACTCAAG GTTTTGATTT TCTTCAAGCC 
ATTGAGCCAG CATTTATTTC AGCTCTCCCA GAAGATGACT TCTTGAGTTT ACAAGCTCTG 
ATGAATGAAT GCATTGGCCA TGTTATTGGA AAACCTCATA GTCCTGTTAC AGCTATTCAT 
CGAAACAGTC CCCGTCCTGT AAAGGTACCT CGATGCCATA GTGATCCACC AAATCCTCAT 
CTTATCATCC CCACTCCTGA AGGTTTCAGT TTTTCTTCTT GCCACAGCAC GCGGAGTGTC 
CCTTGTGATG CACGGAACCA CTGCAGCGCT GCTGTCAGTC GCCCCGCTCC CATTGGTAGC 
GACTCAGCTC AACCCAAACC TGTGAGCAGT GCAAATGAGA CCAGGGGTTC CAGTGTCCCT 
GAAAATGATC GACTGGCTTC AATAGCAGCT GAGTTGCAGT TCAGATCCCT TAGTCGTCAC 
TCCAGTCCCA CTGAAGAGCG AGAAGAACCA TCTTATCCAA AAGGAGATTC AGGTGGATCA 
TCCCGGCGGA GTTGGGAGCT CAGAACTTTA ATCAACCAGA CCAAAGACAC TGCTTCCAAG 
CAAGCGCCAA TCGAAGCTGT TCAGAAGTCT GTTCAGTTGT TTGAAGAAAG GAGATACAGA 
GAGATGAGGA GGAAGAACAT CATTGGCCAA GTTTGTGACA CCCCAAAATC TTATGATAAT 
GTCATGCATG TAGGTTTGAG GAAAGTGACT TTCAAGTGGC AGAGAGGAAA CAAAATTGGT 
GAAGGCCAGT ACGGAAAAGT CTACACCTGT ATCAGTGTTG ACACTGGTGA ACTGATGGCT 
ATGAAAGAAA TAAGATTTCA ACCCAATGAT CACAAAACCA TCAAAGAAAC AGCAGATGAA 
CTGAAGATTT TTGAAGGCAT CAAACACCCT AATCTTGTTC GTTACTTTGG TGTGGAGCTC 
CATAGGGAAG AAATGTACAT CTTCATGGAG TACTGTGATG AGGGCACCCT GGAGGAGGTA 
TCAAAACTGG GCCTTCAGGA ACATGTCATT AGGCTCTACT CAAAGCAAAT CACAACTGCT 
ATCAATGTAC TGCATGAACA TGGTATTGTT CATCGAGACA TTAAAGGTAA TGGTAACCAG 
AAAAGGAGCC AACATCTTTC TTACCTCATC TGGACTGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013800904.1
CDS	140..4501
Translation	MRDVAAVALVSAAAAMEEPPPPGRTECKSEDATCKISESEPEELSDEASADTFYGTSPPSTPRQMKRMSTKHQKNNVGRPASRSTLKEKMGLPTQSVHKDNGKTIENVEEHSYKQGKKNRATLRTTERDHKKNVQCSFMLDSVGGSLPKKTIPDVDLNKPYLSLGCSNAKLPVSVPMPIARTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDREQRGQENTSSVWLNRSNELIWLELQAWHAGRSINDQDLYLYAARQAIPDIINEILTFKVNYGSFSCVKNGASLNGTTGEGVCKATHGTSALGYSSYHEHLHRQRVSFEQVKRIMELLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPGPSKDNDPEDEEVDRETEVKESSGMCTEESDGEEQISEENVEELRQAIKNNFTNKPNFDFQDHFSRKLDRLESEDDSASWVIPECSAEAGCSKHCLTSIYRPFVDKALKQMGLRKLILRLHKLMHGSLQRARMALVRNDHQLEFSEFPDPMLCSDYVQLLNSPPSCEKKCSTVSWEELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLREVLDDLQKIDCNIDSFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITPYIRGGEAQAGKLFCDIAGMLLKSTGIFLDSGLQDSCDEFWASADDSTASDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFALSAPVRDFLNALKTKEYVKVQIPGLENLQVFVPNSIAGEKSVILQLLNAAAGKDCSKDSDEVAYEAYLLMTKHSDKDHELDNSWSAWEGQPVKVVPQVETADTLRSMQVDNLLLVVIQSAHLVSQRKAFQQSIEGLISLHHEQTSSQPVIAKALQQLKSDALQLCNKISSAIDRVDRMFTSEFDAEVDESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFSFSSCHSTRSVPCDARNHCSAAVSRPAPIGSDSAQPKPVSSANETRGSSVPENDRLASIAAELQFRSLSRHSSPTEEREEPSYPKGDSGGSSRRSWELRTLINQTKDTASKQAPIEAVQKSVQLFEERRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSKLGLQEHVIRLYSKQITTAINVLHEHGIVHRDIKGNGNQKRSQHLSYLIWTD

Target ORF information:

RefSeq Version	XM_013945450.1
Organism	Apteryx australis mantelli
Definition	Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013945450.1

ORF Insert Sequence:

ATGAGAGACG TCGCCGCCGT CGCGCTGGTC TCTGCTGCCG CCGCCATGGA GGAGCCGCCG 
CCGCCGGGGA GGACAGAGTG CAAATCAGAA GATGCTACTT GCAAGATCTC TGAATCTGAA 
CCTGAGGAAT TATCAGATGA AGCAAGTGCT GACACTTTCT ATGGAACTTC TCCTCCTAGT 
ACGCCCCGAC AGATGAAACG TATGTCAACA AAGCATCAGA AAAATAATGT TGGGAGACCT 
GCTAGCCGTT CCACTTTGAA AGAAAAAATG GGTTTACCAA CTCAGTCTGT GCATAAAGAC 
AATGGGAAAA CCATTGAGAA TGTGGAAGAG CACAGCTATA AGCAAGGGAA GAAGAACCGA 
GCCACCCTAA GGACAACAGA ACGAGATCAC AAGAAAAATG TGCAGTGCTC ATTTATGTTA 
GACTCAGTTG GTGGGTCTTT GCCAAAAAAA ACAATTCCAG ATGTTGATCT CAATAAGCCT 
TACCTCAGCC TTGGCTGTAG CAATGCTAAG CTTCCAGTCT CTGTGCCCAT GCCAATAGCC 
AGAACTGCAC GCCAGACTTC TAGGACTGAT TGCCCAGCAG ACCGCTTAAA ATTCTTTGAA 
ACCCTGCGGC TTTTGTTAAA ACTTACCTCA GTCTCGAAGA AAAAGGACAG GGAGCAAAGA 
GGACAGGAAA ACACCTCTTC TGTCTGGCTG AACCGATCCA ATGAACTGAT CTGGCTGGAG 
CTGCAAGCTT GGCATGCTGG CCGGAGCATT AATGACCAAG ACCTATATCT CTATGCGGCC 
CGTCAAGCCA TCCCTGATAT TATCAATGAA ATCCTCACCT TCAAAGTAAA CTATGGAAGC 
TTCTCCTGTG TAAAAAATGG AGCCAGTCTC AATGGTACTA CAGGAGAAGG AGTTTGCAAA 
GCAACACATG GAACTAGTGC TTTGGGTTAC TCAAGTTACC ATGAACACCT CCATCGCCAG 
CGGGTCTCAT TTGAGCAAGT AAAGCGGATA ATGGAGCTGC TAGAGTATAT AGAAGCACTT 
TATCCATCTC TGCAGGCTCT TCAAAAGGAC TATGAAAAGT ATGCTGCAAA GGACTTCCAA 
GACAGAGTGC AGGCACTCTG TCTGTGGTTA AACATTACAA AAGACTTAAA TCAAAAACTA 
AGGATTATGG GAACTGTTTT GGGCATCAAA AATCTTTCTG ACATTGGCTG GCCAGTGTTT 
GAAATTCCTT CTCCTGGACC GTCTAAGGAC AATGATCCAG AGGATGAGGA AGTTGATAGG 
GAAACAGAAG TAAAGGAATC CTCAGGAATG TGCACAGAGG AGAGTGATGG TGAAGAACAA 
ATCTCTGAGG AAAATGTTGA GGAACTTAGA CAAGCCATCA AGAACAATTT TACTAATAAG 
CCAAATTTTG ACTTTCAGGA CCATTTTTCA AGGAAGCTTG ATAGGCTTGA ATCTGAGGAT 
GACTCTGCCT CTTGGGTTAT TCCAGAATGC AGTGCTGAGG CAGGCTGCAG CAAACACTGT 
TTGACCTCTA TTTACAGGCC ATTTGTAGAT AAAGCACTGA AGCAAATGGG GTTGAGGAAG 
CTAATTTTGA GACTGCACAA GCTAATGCAT GGTTCATTGC AAAGGGCCCG TATGGCGTTG 
GTAAGGAACG ATCACCAGCT GGAGTTTTCA GAATTTCCAG ATCCCATGTT GTGTTCAGAT 
TATGTGCAGT TATTAAATTC CCCGCCTTCC TGTGAGAAAA AATGCAGCAC TGTATCATGG 
GAAGAGCTGA AATCTATGGA TTTACCATCT TTTGAACCTG CATTCTTGGT CCTTTGCCGA 
GTCCTGCTCA ATGTTATCCA TGAATGTCTC AAGCTAAGGT TGGAACAAAG ACCTGCTGGG 
GAACCATCTC TTTTGAGTAT TAAGCAGCTA GTAAGAGAGT GTAAAGAGGT CCTGAAGGGC 
GGCCTGTTAA TGAAGCAATA TTATCAGTTC ATGTTACGTG AGGTGTTAGA TGACTTGCAA 
AAGATTGACT GCAACATTGA TTCTTTTGAA GAGGACCTGC ATAAAATGTT AATGGTTTAC 
TTTGATTATA TGAGAAGCTG GATCCAAATG CTACAGCAGT TACCTCAAGC ATCTCATAGT 
TTGAAAAATC TCTTAGAAGA AGAATGGAAT TTCACCAAAG AAATAACTCC TTATATCAGA 
GGAGGTGAAG CTCAAGCTGG AAAACTATTC TGTGATATTG CAGGAATGCT GCTGAAATCT 
ACAGGAATAT TTTTAGACTC TGGACTACAG GACAGTTGTG ATGAGTTTTG GGCCAGTGCT 
GATGATAGTA CTGCATCAGA TGAAATCAGG AGGTCTGTTA TAGAAACCAG CCGAGCACTA 
AAAGAACTCT TTCATGAAGC TAGAGAAAGG GCATCCAAAG CTCTTGGTTT TGCTAAAATG 
CTGAGGAAGG ATCTTGAAAT AGCAGCAGAG TTTGCATTGT CAGCCCCTGT GCGAGATTTT 
CTGAATGCTC TGAAAACAAA AGAGTATGTG AAGGTGCAAA TCCCTGGACT TGAAAACTTA 
CAAGTATTTG TCCCAAATAG TATTGCAGGG GAAAAATCTG TGATTTTACA GCTCCTAAAT 
GCAGCTGCAG GAAAGGATTG CTCGAAGGAT TCGGATGAGG TTGCATATGA GGCCTATTTA 
CTAATGACCA AACACAGTGA CAAAGACCAC GAATTAGACA ACAGTTGGAG CGCGTGGGAG 
GGTCAGCCAG TCAAAGTAGT GCCACAGGTG GAAACTGCTG ATACACTGCG AAGCATGCAG 
GTTGATAACC TCCTCCTTGT AGTCATTCAA TCTGCCCATC TTGTGAGTCA GAGAAAAGCT 
TTCCAACAGT CTATTGAAGG GCTCATATCT TTGCACCACG AACAGACATC CAGCCAACCA 
GTCATTGCCA AAGCTCTGCA ACAGCTAAAG AGTGATGCAT TACAGCTATG CAATAAGATA 
AGCAGTGCCA TTGATAGAGT TGATCGCATG TTCACATCTG AGTTTGATGC TGAAGTTGAT 
GAATCTGAAT CTGCCACTTT GCAACAATAC TACCGGGAAG CCATGATTCA GGGTTATAAT 
TTTGGATTTG AGTACCATAA AGAAGTTGTT CGCCTAATGT CTGGAGAATT CAGGCAGAAA 
ATTGGAGATA AGTATATCAG CTTTGCTAGA AAATGGATGA ATTATGTGCT AACAAAGTGT 
GAGAGTGGCC GAGGCACTCG ACCCAGGTGG GCAACTCAAG GTTTTGATTT TCTTCAAGCC 
ATTGAGCCAG CATTTATTTC AGCTCTCCCA GAAGATGACT TCTTGAGTTT ACAAGCTCTG 
ATGAATGAAT GCATTGGCCA TGTTATTGGA AAACCTCATA GTCCTGTTAC AGCTATTCAT 
CGAAACAGTC CCCGTCCTGT AAAGGTACCT CGATGCCATA GTGATCCACC AAATCCTCAT 
CTTATCATCC CCACTCCTGA AGGTTTCAGT TTTTCTTCTT GCCACAGCAC GCGGAGTGTC 
CCTTGTGATG CACGGAACCA CTGCAGCGCT GCTGTCAGTC GCCCCGCTCC CATTGGTAGC 
GACTCAGCTC AACCCAAACC TGTGAGCAGT GCAAATGAGA CCAGGGGTTC CAGTGTCCCT 
GAAAATGATC GACTGGCTTC AATAGCAGCT GAGTTGCAGT TCAGATCCCT TAGTCGTCAC 
TCCAGTCCCA CTGAAGAGCG AGAAGAACCA TCTTATCCAA AAGGAGATTC AGGTGGATCA 
TCCCGGCGGA GTTGGGAGCT CAGAACTTTA ATCAACCAGA CCAAAGACAC TGCTTCCAAG 
CAAGCGCCAA TCGAAGCTGT TCAGAAGTCT GTTCAGTTGT TTGAAGAAAG GAGATACAGA 
GAGATGAGGA GGAAGAACAT CATTGGCCAA GTTTGTGACA CCCCAAAATC TTATGATAAT 
GTCATGCATG TAGGTTTGAG GAAAGTGACT TTCAAGTGGC AGAGAGGAAA CAAAATTGGT 
GAAGGCCAGT ACGGAAAAGT CTACACCTGT ATCAGTGTTG ACACTGGTGA ACTGATGGCT 
ATGAAAGAAA TAAGATTTCA ACCCAATGAT CACAAAACCA TCAAAGAAAC AGCAGATGAA 
CTGAAGATTT TTGAAGGCAT CAAACACCCT AATCTTGTTC GTTACTTTGG TGTGGAGCTC 
CATAGGGAAG AAATGTACAT CTTCATGGAG TACTGTGATG AGGGCACCCT GGAGGAGGTA 
TCAAAACTGG GCCTTCAGGA ACATGTCATT AGGCTCTACT CAAAGCAAAT CACAACTGCT 
ATCAATGTAC TGCATGAACA TGGTATTGTT CATCGAGACA TTAAAGGTAA TGGTAACCAG 
AAAAGGAGCC AACATCTTTC TTACCTCATC TGGACTGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_013945442.1 ) cDNA ORF clone, Apteryx australis mantelli -> XP_013800896.1 Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X1, mRNA.

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CloneID	OAp16191
Clone ID Related Accession (Same CDS sequence)	XM_013945442.1
Accession Version	XM_013945442.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-08
Organism	Apteryx australis mantelli
Product	mitogen-activated protein kinase kinase kinase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013993955.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apteryx australis mantelli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGACG TCGCCGCCGT CGCGCTGGTC TCTGCTGCCG CCGCCATGGA GGAGCCGCCG 
CCGCCGGGGA GGACAGAGTG CAAATCAGAA GATGCTACTT GCAAGATCTC TGAATCTGAA 
CCTGAGGAAT TATCAGATGA AGCAAGTGCT GACACTTTCT ATGGAACTTC TCCTCCTAGT 
ACGCCCCGAC AGATGAAACG TATGTCAACA AAGCATCAGA AAAATAATGT TGGGAGACCT 
GCTAGCCGTT CCACTTTGAA AGAAAAAATG GGTTTACCAA CTCAGTCTGT GCATAAAGAC 
AATGGGAAAA CCATTGAGAA TGTGGAAGAG CACAGCTATA AGCAAGGGAA GAAGAACCGA 
GCCACCCTAA GGACAACAGA ACGAGATCAC AAGAAAAATG TGCAGTGCTC ATTTATGTTA 
GACTCAGTTG GTGGGTCTTT GCCAAAAAAA ACAATTCCAG ATGTTGATCT CAATAAGCCT 
TACCTCAGCC TTGGCTGTAG CAATGCTAAG CTTCCAGTCT CTGTGCCCAT GCCAATAGCC 
AGAACTGCAC GCCAGACTTC TAGGACTGAT TGCCCAGCAG ACCGCTTAAA ATTCTTTGAA 
ACCCTGCGGC TTTTGTTAAA ACTTACCTCA GTCTCGAAGA AAAAGGACAG GGAGCAAAGA 
GGACAGGAAA ACACCTCTTC TGTCTGGCTG AACCGATCCA ATGAACTGAT CTGGCTGGAG 
CTGCAAGCTT GGCATGCTGG CCGGAGCATT AATGACCAAG ACCTATATCT CTATGCGGCC 
CGTCAAGCCA TCCCTGATAT TATCAATGAA ATCCTCACCT TCAAAGTAAA CTATGGAAGC 
TTCTCCTGTG TAAAAAATGG AGCCAGTCTC AATGGTACTA CAGGAGAAGG AGTTTGCAAA 
GCAACACATG GAACTAGTGC TTTGGGTTAC TCAAGTTACC ATGAACACCT CCATCGCCAG 
CGGGTCTCAT TTGAGCAAGT AAAGCGGATA ATGGAGCTGC TAGAGTATAT AGAAGCACTT 
TATCCATCTC TGCAGGCTCT TCAAAAGGAC TATGAAAAGT ATGCTGCAAA GGACTTCCAA 
GACAGAGTGC AGGCACTCTG TCTGTGGTTA AACATTACAA AAGACTTAAA TCAAAAACTA 
AGGATTATGG GAACTGTTTT GGGCATCAAA AATCTTTCTG ACATTGGCTG GCCAGTGTTT 
GAAATTCCTT CTCCTGGACC GTCTAAGGAC AATGATCCAG AGGATGAGGA AGTTGATAGG 
GAAACAGAAG TAAAGGAATC CTCAGGAATG TGCACAGAGG AGAGTGATGG TGAAGAACAA 
ATCTCTGAGG AAAATGTTGA GGAACTTAGA CAAGCCATCA AGAACAATTT TACTAATAAG 
CCAAATTTTG ACTTTCAGGA CCATTTTTCA AGGAAGCTTG ATAGGCTTGA ATCTGAGGAT 
GACTCTGCCT CTTGGGTTAT TCCAGAATGC AGTGCTGAGG CAGGCTGCAG CAAACACTGT 
TTGACCTCTA TTTACAGGCC ATTTGTAGAT AAAGCACTGA AGCAAATGGG GTTGAGGAAG 
CTAATTTTGA GACTGCACAA GCTAATGCAT GGTTCATTGC AAAGGGCCCG TATGGCGTTG 
GTAAGGAACG ATCACCAGCT GGAGTTTTCA GAATTTCCAG ATCCCATGTT GTGTTCAGAT 
TATGTGCAGT TATTAAATTC CCCGCCTTCC TGTGAGAAAA AATGCAGCAC TGTATCATGG 
GAAGAGCTGA AATCTATGGA TTTACCATCT TTTGAACCTG CATTCTTGGT CCTTTGCCGA 
GTCCTGCTCA ATGTTATCCA TGAATGTCTC AAGCTAAGGT TGGAACAAAG ACCTGCTGGG 
GAACCATCTC TTTTGAGTAT TAAGCAGCTA GTAAGAGAGT GTAAAGAGGT CCTGAAGGGC 
GGCCTGTTAA TGAAGCAATA TTATCAGTTC ATGTTACGTG AGGTGTTAGA TGACTTGCAA 
AAGATTGACT GCAACATTGA TTCTTTTGAA GAGGACCTGC ATAAAATGTT AATGGTTTAC 
TTTGATTATA TGAGAAGCTG GATCCAAATG CTACAGCAGT TACCTCAAGC ATCTCATAGT 
TTGAAAAATC TCTTAGAAGA AGAATGGAAT TTCACCAAAG AAATAACTCC TTATATCAGA 
GGAGGTGAAG CTCAAGCTGG AAAACTATTC TGTGATATTG CAGGAATGCT GCTGAAATCT 
ACAGGAATAT TTTTAGACTC TGGACTACAG GACAGTTGTG ATGAGTTTTG GGCCAGTGCT 
GATGATAGTA CTGCATCAGA TGAAATCAGG AGGTCTGTTA TAGAAACCAG CCGAGCACTA 
AAAGAACTCT TTCATGAAGC TAGAGAAAGG GCATCCAAAG CTCTTGGTTT TGCTAAAATG 
CTGAGGAAGG ATCTTGAAAT AGCAGCAGAG TTTGCATTGT CAGCCCCTGT GCGAGATTTT 
CTGAATGCTC TGAAAACAAA AGAGTATGTG AAGGTGCAAA TCCCTGGACT TGAAAACTTA 
CAAGTATTTG TCCCAAATAG TATTGCAGGG GAAAAATCTG TGATTTTACA GCTCCTAAAT 
GCAGCTGCAG GAAAGGATTG CTCGAAGGAT TCGGATGAGG TTGCATATGA GGCCTATTTA 
CTAATGACCA AACACAGTGA CAAAGACCAC GAATTAGACA ACAGTTGGAG CGCGTGGGAG 
GGTCAGCCAG TCAAAGTAGT GCCACAGGTG GAAACTGCTG ATACACTGCG AAGCATGCAG 
GTTGATAACC TCCTCCTTGT AGTCATTCAA TCTGCCCATC TTGTGAGTCA GAGAAAAGCT 
TTCCAACAGT CTATTGAAGG GCTCATATCT TTGCACCACG AACAGACATC CAGCCAACCA 
GTCATTGCCA AAGCTCTGCA ACAGCTAAAG AGTGATGCAT TACAGCTATG CAATAAGATA 
AGCAGTGCCA TTGATAGAGT TGATCGCATG TTCACATCTG AGTTTGATGC TGAAGTTGAT 
GAATCTGAAT CTGCCACTTT GCAACAATAC TACCGGGAAG CCATGATTCA GGGTTATAAT 
TTTGGATTTG AGTACCATAA AGAAGTTGTT CGCCTAATGT CTGGAGAATT CAGGCAGAAA 
ATTGGAGATA AGTATATCAG CTTTGCTAGA AAATGGATGA ATTATGTGCT AACAAAGTGT 
GAGAGTGGCC GAGGCACTCG ACCCAGGTGG GCAACTCAAG GTTTTGATTT TCTTCAAGCC 
ATTGAGCCAG CATTTATTTC AGCTCTCCCA GAAGATGACT TCTTGAGTTT ACAAGCTCTG 
ATGAATGAAT GCATTGGCCA TGTTATTGGA AAACCTCATA GTCCTGTTAC AGCTATTCAT 
CGAAACAGTC CCCGTCCTGT AAAGGTACCT CGATGCCATA GTGATCCACC AAATCCTCAT 
CTTATCATCC CCACTCCTGA AGGTTTCAGT TTTTCTTCTT GCCACAGCAC GCGGAGTGTC 
CCTTGTGATG CACGGAACCA CTGCAGCGCT GCTGTCAGTC GCCCCGCTCC CATTGGTAGC 
GACTCAGCTC AACCCAAACC TGTGAGCAGT GCAAATGAGA CCAGGGGTTC CAGTGTCCCT 
GAAAATGATC GACTGGCTTC AATAGCAGCT GAGTTGCAGT TCAGATCCCT TAGTCGTCAC 
TCCAGTCCCA CTGAAGAGCG AGAAGAACCA TCTTATCCAA AAGGAGATTC AGGTGGATCA 
TCCCGGCGGA GTTGGGAGCT CAGAACTTTA ATCAACCAGA CCAAAGACAC TGCTTCCAAG 
CAAGCGCCAA TCGAAGCTGT TCAGAAGTCT GTTCAGTTGT TTGAAGAAAG GAGATACAGA 
GAGATGAGGA GGAAGAACAT CATTGGCCAA GTTTGTGACA CCCCAAAATC TTATGATAAT 
GTCATGCATG TAGGTTTGAG GAAAGTGACT TTCAAGTGGC AGAGAGGAAA CAAAATTGGT 
GAAGGCCAGT ACGGAAAAGT CTACACCTGT ATCAGTGTTG ACACTGGTGA ACTGATGGCT 
ATGAAAGAAA TAAGATTTCA ACCCAATGAT CACAAAACCA TCAAAGAAAC AGCAGATGAA 
CTGAAGATTT TTGAAGGCAT CAAACACCCT AATCTTGTTC GTTACTTTGG TGTGGAGCTC 
CATAGGGAAG AAATGTACAT CTTCATGGAG TACTGTGATG AGGGCACCCT GGAGGAGGTA 
TCAAAACTGG GCCTTCAGGA ACATGTCATT AGGCTCTACT CAAAGCAAAT CACAACTGCT 
ATCAATGTAC TGCATGAACA TGGTATTGTT CATCGAGACA TTAAAGGAGC CAACATCTTT 
CTTACCTCAT CTGGACTGAT TAAATTAGGA GACTTTGGCT GCTCAGTGAA ATTAAAGAAT 
AACACCCAGA CAATGCCTGG AGAAGTGAAC AGTACTTTGG GGACTGCAGC ATACATGGCT 
CCAGAAGTCA TTACTAGAGC TAAAGGAGAA GGGCATGGGC GTGCAGCAGA CATCTGGAGC 
CTGGGCTGTG TTGTAATAGA GATGGTCACT GGCAAGAGGC CGTGGCATGA ATACGAGCAC 
AACTTCCAGA TCATGTATAG AGTGGGGATG GGTCATAAAC CTCCTATTCC TGACAAGGTG 
AGCCCAGAAG GGAAAGACTT CCTTTGCCAC TGTCTGGAAA GTGACCCAAA GATGAGGTGG 
ACGGCTAGCC AGCTCCTCGA CCATCCCTTT GTCAAGGTTT GCACAGATGA AGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013800896.1
CDS	140..4876
Translation	MRDVAAVALVSAAAAMEEPPPPGRTECKSEDATCKISESEPEELSDEASADTFYGTSPPSTPRQMKRMSTKHQKNNVGRPASRSTLKEKMGLPTQSVHKDNGKTIENVEEHSYKQGKKNRATLRTTERDHKKNVQCSFMLDSVGGSLPKKTIPDVDLNKPYLSLGCSNAKLPVSVPMPIARTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDREQRGQENTSSVWLNRSNELIWLELQAWHAGRSINDQDLYLYAARQAIPDIINEILTFKVNYGSFSCVKNGASLNGTTGEGVCKATHGTSALGYSSYHEHLHRQRVSFEQVKRIMELLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPGPSKDNDPEDEEVDRETEVKESSGMCTEESDGEEQISEENVEELRQAIKNNFTNKPNFDFQDHFSRKLDRLESEDDSASWVIPECSAEAGCSKHCLTSIYRPFVDKALKQMGLRKLILRLHKLMHGSLQRARMALVRNDHQLEFSEFPDPMLCSDYVQLLNSPPSCEKKCSTVSWEELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLREVLDDLQKIDCNIDSFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITPYIRGGEAQAGKLFCDIAGMLLKSTGIFLDSGLQDSCDEFWASADDSTASDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFALSAPVRDFLNALKTKEYVKVQIPGLENLQVFVPNSIAGEKSVILQLLNAAAGKDCSKDSDEVAYEAYLLMTKHSDKDHELDNSWSAWEGQPVKVVPQVETADTLRSMQVDNLLLVVIQSAHLVSQRKAFQQSIEGLISLHHEQTSSQPVIAKALQQLKSDALQLCNKISSAIDRVDRMFTSEFDAEVDESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFSFSSCHSTRSVPCDARNHCSAAVSRPAPIGSDSAQPKPVSSANETRGSSVPENDRLASIAAELQFRSLSRHSSPTEEREEPSYPKGDSGGSSRRSWELRTLINQTKDTASKQAPIEAVQKSVQLFEERRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSKLGLQEHVIRLYSKQITTAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNTQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYRVGMGHKPPIPDKVSPEGKDFLCHCLESDPKMRWTASQLLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_013945442.1
Organism	Apteryx australis mantelli
Definition	Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013945442.1

ORF Insert Sequence:

ATGAGAGACG TCGCCGCCGT CGCGCTGGTC TCTGCTGCCG CCGCCATGGA GGAGCCGCCG 
CCGCCGGGGA GGACAGAGTG CAAATCAGAA GATGCTACTT GCAAGATCTC TGAATCTGAA 
CCTGAGGAAT TATCAGATGA AGCAAGTGCT GACACTTTCT ATGGAACTTC TCCTCCTAGT 
ACGCCCCGAC AGATGAAACG TATGTCAACA AAGCATCAGA AAAATAATGT TGGGAGACCT 
GCTAGCCGTT CCACTTTGAA AGAAAAAATG GGTTTACCAA CTCAGTCTGT GCATAAAGAC 
AATGGGAAAA CCATTGAGAA TGTGGAAGAG CACAGCTATA AGCAAGGGAA GAAGAACCGA 
GCCACCCTAA GGACAACAGA ACGAGATCAC AAGAAAAATG TGCAGTGCTC ATTTATGTTA 
GACTCAGTTG GTGGGTCTTT GCCAAAAAAA ACAATTCCAG ATGTTGATCT CAATAAGCCT 
TACCTCAGCC TTGGCTGTAG CAATGCTAAG CTTCCAGTCT CTGTGCCCAT GCCAATAGCC 
AGAACTGCAC GCCAGACTTC TAGGACTGAT TGCCCAGCAG ACCGCTTAAA ATTCTTTGAA 
ACCCTGCGGC TTTTGTTAAA ACTTACCTCA GTCTCGAAGA AAAAGGACAG GGAGCAAAGA 
GGACAGGAAA ACACCTCTTC TGTCTGGCTG AACCGATCCA ATGAACTGAT CTGGCTGGAG 
CTGCAAGCTT GGCATGCTGG CCGGAGCATT AATGACCAAG ACCTATATCT CTATGCGGCC 
CGTCAAGCCA TCCCTGATAT TATCAATGAA ATCCTCACCT TCAAAGTAAA CTATGGAAGC 
TTCTCCTGTG TAAAAAATGG AGCCAGTCTC AATGGTACTA CAGGAGAAGG AGTTTGCAAA 
GCAACACATG GAACTAGTGC TTTGGGTTAC TCAAGTTACC ATGAACACCT CCATCGCCAG 
CGGGTCTCAT TTGAGCAAGT AAAGCGGATA ATGGAGCTGC TAGAGTATAT AGAAGCACTT 
TATCCATCTC TGCAGGCTCT TCAAAAGGAC TATGAAAAGT ATGCTGCAAA GGACTTCCAA 
GACAGAGTGC AGGCACTCTG TCTGTGGTTA AACATTACAA AAGACTTAAA TCAAAAACTA 
AGGATTATGG GAACTGTTTT GGGCATCAAA AATCTTTCTG ACATTGGCTG GCCAGTGTTT 
GAAATTCCTT CTCCTGGACC GTCTAAGGAC AATGATCCAG AGGATGAGGA AGTTGATAGG 
GAAACAGAAG TAAAGGAATC CTCAGGAATG TGCACAGAGG AGAGTGATGG TGAAGAACAA 
ATCTCTGAGG AAAATGTTGA GGAACTTAGA CAAGCCATCA AGAACAATTT TACTAATAAG 
CCAAATTTTG ACTTTCAGGA CCATTTTTCA AGGAAGCTTG ATAGGCTTGA ATCTGAGGAT 
GACTCTGCCT CTTGGGTTAT TCCAGAATGC AGTGCTGAGG CAGGCTGCAG CAAACACTGT 
TTGACCTCTA TTTACAGGCC ATTTGTAGAT AAAGCACTGA AGCAAATGGG GTTGAGGAAG 
CTAATTTTGA GACTGCACAA GCTAATGCAT GGTTCATTGC AAAGGGCCCG TATGGCGTTG 
GTAAGGAACG ATCACCAGCT GGAGTTTTCA GAATTTCCAG ATCCCATGTT GTGTTCAGAT 
TATGTGCAGT TATTAAATTC CCCGCCTTCC TGTGAGAAAA AATGCAGCAC TGTATCATGG 
GAAGAGCTGA AATCTATGGA TTTACCATCT TTTGAACCTG CATTCTTGGT CCTTTGCCGA 
GTCCTGCTCA ATGTTATCCA TGAATGTCTC AAGCTAAGGT TGGAACAAAG ACCTGCTGGG 
GAACCATCTC TTTTGAGTAT TAAGCAGCTA GTAAGAGAGT GTAAAGAGGT CCTGAAGGGC 
GGCCTGTTAA TGAAGCAATA TTATCAGTTC ATGTTACGTG AGGTGTTAGA TGACTTGCAA 
AAGATTGACT GCAACATTGA TTCTTTTGAA GAGGACCTGC ATAAAATGTT AATGGTTTAC 
TTTGATTATA TGAGAAGCTG GATCCAAATG CTACAGCAGT TACCTCAAGC ATCTCATAGT 
TTGAAAAATC TCTTAGAAGA AGAATGGAAT TTCACCAAAG AAATAACTCC TTATATCAGA 
GGAGGTGAAG CTCAAGCTGG AAAACTATTC TGTGATATTG CAGGAATGCT GCTGAAATCT 
ACAGGAATAT TTTTAGACTC TGGACTACAG GACAGTTGTG ATGAGTTTTG GGCCAGTGCT 
GATGATAGTA CTGCATCAGA TGAAATCAGG AGGTCTGTTA TAGAAACCAG CCGAGCACTA 
AAAGAACTCT TTCATGAAGC TAGAGAAAGG GCATCCAAAG CTCTTGGTTT TGCTAAAATG 
CTGAGGAAGG ATCTTGAAAT AGCAGCAGAG TTTGCATTGT CAGCCCCTGT GCGAGATTTT 
CTGAATGCTC TGAAAACAAA AGAGTATGTG AAGGTGCAAA TCCCTGGACT TGAAAACTTA 
CAAGTATTTG TCCCAAATAG TATTGCAGGG GAAAAATCTG TGATTTTACA GCTCCTAAAT 
GCAGCTGCAG GAAAGGATTG CTCGAAGGAT TCGGATGAGG TTGCATATGA GGCCTATTTA 
CTAATGACCA AACACAGTGA CAAAGACCAC GAATTAGACA ACAGTTGGAG CGCGTGGGAG 
GGTCAGCCAG TCAAAGTAGT GCCACAGGTG GAAACTGCTG ATACACTGCG AAGCATGCAG 
GTTGATAACC TCCTCCTTGT AGTCATTCAA TCTGCCCATC TTGTGAGTCA GAGAAAAGCT 
TTCCAACAGT CTATTGAAGG GCTCATATCT TTGCACCACG AACAGACATC CAGCCAACCA 
GTCATTGCCA AAGCTCTGCA ACAGCTAAAG AGTGATGCAT TACAGCTATG CAATAAGATA 
AGCAGTGCCA TTGATAGAGT TGATCGCATG TTCACATCTG AGTTTGATGC TGAAGTTGAT 
GAATCTGAAT CTGCCACTTT GCAACAATAC TACCGGGAAG CCATGATTCA GGGTTATAAT 
TTTGGATTTG AGTACCATAA AGAAGTTGTT CGCCTAATGT CTGGAGAATT CAGGCAGAAA 
ATTGGAGATA AGTATATCAG CTTTGCTAGA AAATGGATGA ATTATGTGCT AACAAAGTGT 
GAGAGTGGCC GAGGCACTCG ACCCAGGTGG GCAACTCAAG GTTTTGATTT TCTTCAAGCC 
ATTGAGCCAG CATTTATTTC AGCTCTCCCA GAAGATGACT TCTTGAGTTT ACAAGCTCTG 
ATGAATGAAT GCATTGGCCA TGTTATTGGA AAACCTCATA GTCCTGTTAC AGCTATTCAT 
CGAAACAGTC CCCGTCCTGT AAAGGTACCT CGATGCCATA GTGATCCACC AAATCCTCAT 
CTTATCATCC CCACTCCTGA AGGTTTCAGT TTTTCTTCTT GCCACAGCAC GCGGAGTGTC 
CCTTGTGATG CACGGAACCA CTGCAGCGCT GCTGTCAGTC GCCCCGCTCC CATTGGTAGC 
GACTCAGCTC AACCCAAACC TGTGAGCAGT GCAAATGAGA CCAGGGGTTC CAGTGTCCCT 
GAAAATGATC GACTGGCTTC AATAGCAGCT GAGTTGCAGT TCAGATCCCT TAGTCGTCAC 
TCCAGTCCCA CTGAAGAGCG AGAAGAACCA TCTTATCCAA AAGGAGATTC AGGTGGATCA 
TCCCGGCGGA GTTGGGAGCT CAGAACTTTA ATCAACCAGA CCAAAGACAC TGCTTCCAAG 
CAAGCGCCAA TCGAAGCTGT TCAGAAGTCT GTTCAGTTGT TTGAAGAAAG GAGATACAGA 
GAGATGAGGA GGAAGAACAT CATTGGCCAA GTTTGTGACA CCCCAAAATC TTATGATAAT 
GTCATGCATG TAGGTTTGAG GAAAGTGACT TTCAAGTGGC AGAGAGGAAA CAAAATTGGT 
GAAGGCCAGT ACGGAAAAGT CTACACCTGT ATCAGTGTTG ACACTGGTGA ACTGATGGCT 
ATGAAAGAAA TAAGATTTCA ACCCAATGAT CACAAAACCA TCAAAGAAAC AGCAGATGAA 
CTGAAGATTT TTGAAGGCAT CAAACACCCT AATCTTGTTC GTTACTTTGG TGTGGAGCTC 
CATAGGGAAG AAATGTACAT CTTCATGGAG TACTGTGATG AGGGCACCCT GGAGGAGGTA 
TCAAAACTGG GCCTTCAGGA ACATGTCATT AGGCTCTACT CAAAGCAAAT CACAACTGCT 
ATCAATGTAC TGCATGAACA TGGTATTGTT CATCGAGACA TTAAAGGAGC CAACATCTTT 
CTTACCTCAT CTGGACTGAT TAAATTAGGA GACTTTGGCT GCTCAGTGAA ATTAAAGAAT 
AACACCCAGA CAATGCCTGG AGAAGTGAAC AGTACTTTGG GGACTGCAGC ATACATGGCT 
CCAGAAGTCA TTACTAGAGC TAAAGGAGAA GGGCATGGGC GTGCAGCAGA CATCTGGAGC 
CTGGGCTGTG TTGTAATAGA GATGGTCACT GGCAAGAGGC CGTGGCATGA ATACGAGCAC 
AACTTCCAGA TCATGTATAG AGTGGGGATG GGTCATAAAC CTCCTATTCC TGACAAGGTG 
AGCCCAGAAG GGAAAGACTT CCTTTGCCAC TGTCTGGAAA GTGACCCAAA GATGAGGTGG 
ACGGCTAGCC AGCTCCTCGA CCATCCCTTT GTCAAGGTTT GCACAGATGA AGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_013945443.1 ) cDNA ORF clone, Apteryx australis mantelli -> XP_013800897.1 Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X2, mRNA.

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CloneID	OAp16192
Clone ID Related Accession (Same CDS sequence)	XM_013945443.1
Accession Version	XM_013945443.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-08
Organism	Apteryx australis mantelli
Product	mitogen-activated protein kinase kinase kinase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013993955.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apteryx australis mantelli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGACG TCGCCGCCGT CGCGCTGGTC TCTGCTGCCG CCGCCATGGA GGAGCCGCCG 
CCGCCGGGGA GGACAGAGTG CAAATCAGAA GATGCTACTT GCAAGATCTC TGAATCTGAA 
CCTGAGGAAT TATCAGATGA AGCAAGTGCT GACACTTTCT ATGGAACTTC TCCTCCTAGT 
ACGCCCCGAC AGATGAAACG TATGTCAACA AAGCATCAGA AAAATAATGT TGGGAGACCT 
GCTAGCCGTT CCACTTTGAA AGAAAAAATG GGTTTACCAA CTCAGTCTGT GCATAAAGAC 
AATGGGAAAA CCATTGAGAA TGTGGAAGAG CACAGCTATA AGCAAGGGAA GAAGAACCGA 
GCCACCCTAA GGACAACAGA ACGAGATCAC AAGAAAAATG TGCAGTGCTC ATTTATGTTA 
GACTCAGTTG GTGGGTCTTT GCCAAAAAAA ACAATTCCAG ATGTTGATCT CAATAAGCCT 
TACCTCAGCC TTGGCTGTAG CAATGCTAAG CTTCCAGTCT CTGTGCCCAT GCCAATAGCC 
AGAACTGCAC GCCAGACTTC TAGGACTGAT TGCCCAGCAG ACCGCTTAAA ATTCTTTGAA 
ACCCTGCGGC TTTTGTTAAA ACTTACCTCA GTCTCGAAGA AAAAGGACAG GGAGCAAAGA 
GGACAGGAAA ACACCTCTTC TGTCTGGCTG AACCGATCCA ATGAACTGAT CTGGCTGGAG 
CTGCAAGCTT GGCATGCTGG CCGGAGCATT AATGACCAAG ACCTATATCT CTATGCGGCC 
CGTCAAGCCA TCCCTGATAT TATCAATGAA ATCCTCACCT TCAAAGTAAA CTATGGAAGC 
TTCTCCTGTG TAAAAAATGG AGCCAGTCTC AATGGTACTA CAGGAGAAGG AGTTTGCAAA 
GCAACACATG GAACTAGTGC TTTGGGTTAC TCAAGTTACC ATGAACACCT CCATCGCCAG 
CGGGTCTCAT TTGAGCAAGT AAAGCGGATA ATGGAGCTGC TAGAGTATAT AGAAGCACTT 
TATCCATCTC TGCAGGCTCT TCAAAAGGAC TATGAAAAGT ATGCTGCAAA GGACTTCCAA 
GACAGAGTGC AGGCACTCTG TCTGTGGTTA AACATTACAA AAGACTTAAA TCAAAAACTA 
AGGATTATGG GAACTGTTTT GGGCATCAAA AATCTTTCTG ACATTGGCTG GCCAGTGTTT 
GAAATTCCTT CTCCTGGACC GTCTAAGGAC AATGATCCAG AGGATGAGGA AGTTGATAGG 
GAAACAGAAG TAAAGGAATC CTCAGGAATG TGCACAGAGG AGAGTGATGG TGAAGAACAA 
ATCTCTGAGG AAAATGTTGA GGAACTTAGA CAAGCCATCA AGAACAATTT TACTAATAAG 
CCAAATTTTG ACTTTCAGGA CCATTTTTCA AGGAAGCTTG ATAGGCTTGA ATCTGAGGAT 
GACTCTGCCT CTTGGGTTAT TCCAGAATGC AGTGCTGAGG CAGGCTGCAG CAAACACTGT 
TTGACCTCTA TTTACAGGCC ATTTGTAGAT AAAGCACTGA AGCAAATGGG GTTGAGGAAG 
CTAATTTTGA GACTGCACAA GCTAATGCAT GGTTCATTGC AAAGGGCCCG TATGGCGTTG 
GTAAGGAACG ATCACCAGCT GGAGTTTTCA GAATTTCCAG ATCCCATGTT GTGTTCAGAT 
TATGTGCAGT TATTAAATTC CCCGCCTTCC TGTGAGAAAA AATGCAGCAC TGTATCATGG 
GAAGAGCTGA AATCTATGGA TTTACCATCT TTTGAACCTG CATTCTTGGT CCTTTGCCGA 
GTCCTGCTCA ATGTTATCCA TGAATGTCTC AAGCTAAGGT TGGAACAAAG ACCTGCTGGG 
GAACCATCTC TTTTGAGTAT TAAGCAGCTA GTAAGAGAGT GTAAAGAGGT CCTGAAGGGC 
GGCCTGTTAA TGAAGCAATA TTATCAGTTC ATGTTACGTG AGGTGTTAGA TGACTTGCAA 
AAGATTGACT GCAACATTGA TTCTTTTGAA GAGGACCTGC ATAAAATGTT AATGGTTTAC 
TTTGATTATA TGAGAAGCTG GATCCAAATG CTACAGCAGT TACCTCAAGC ATCTCATAGT 
TTGAAAAATC TCTTAGAAGA AGAATGGAAT TTCACCAAAG AAATAACTCC TTATATCAGA 
GGAGGTGAAG CTCAAGCTGG AAAACTATTC TGTGATATTG CAGGAATGCT GCTGAAATCT 
ACAGGAATAT TTTTAGACTC TGGACTACAG GACAGTTGTG ATGAGTTTTG GGCCAGTGCT 
GATGATAGTA CTGCATCAGA TGAAATCAGG AGGTCTGTTA TAGAAACCAG CCGAGCACTA 
AAAGAACTCT TTCATGAAGC TAGAGAAAGG GCATCCAAAG CTCTTGGTTT TGCTAAAATG 
CTGAGGAAGG ATCTTGAAAT AGCAGCAGAG TTTGCATTGT CAGCCCCTGT GCGAGATTTT 
CTGAATGCTC TGAAAACAAA AGAGTATGTG AAGGTGCAAA TCCCTGGACT TGAAAACTTA 
CAAGTATTTG TCCCAAATAG TATTGCAGGG GAAAAATCTG TGATTTTACA GCTCCTAAAT 
GCAGCTGCAG GAAAGGATTG CTCGAAGGAT TCGGATGAGG TTGCATATGA GGCCTATTTA 
CTAATGACCA AACACAGTGA CAAAGACCAC GAATTAGACA ACAGTTGGAG CGCGTGGGAG 
GGTCAGCCAG TCAAAGTAGT GCCACAGGTG GAAACTGCTG ATACACTGCG AAGCATGCAG 
GTTGATAACC TCCTCCTTGT AGTCATTCAA TCTGCCCATC TTGTGAGTCA GAGAAAAGCT 
TTCCAACAGT CTATTGAAGG GCTCATATCT TTGCACCACG AACAGACATC CAGCCAACCA 
GTCATTGCCA AAGCTCTGCA ACAGCTAAAG AGTGATGCAT TACAGCTATG CAATAAGATA 
AGCAGTGCCA TTGATAGAGT TGATCGCATG TTCACATCTG AGTTTGATGC TGAAGTTGAT 
GAATCTGAAT CTGCCACTTT GCAACAATAC TACCGGGAAG CCATGATTCA GGGTTATAAT 
TTTGGATTTG AGTACCATAA AGAAGTTGTT CGCCTAATGT CTGGAGAATT CAGGCAGAAA 
ATTGGAGATA AGTATATCAG CTTTGCTAGA AAATGGATGA ATTATGTGCT AACAAAGTGT 
GAGAGTGGCC GAGGCACTCG ACCCAGGTGG GCAACTCAAG GTTTTGATTT TCTTCAAGCC 
ATTGAGCCAG CATTTATTTC AGCTCTCCCA GAAGATGACT TCTTGAGTTT ACAAGCTCTG 
ATGAATGAAT GCATTGGCCA TGTTATTGGA AAACCTCATA GTCCTGTTAC AGGTTTGTAC 
CTTGCTATTC ATCGAAACAG TCCCCGTCCT GTAAAGGTAC CTCGATGCCA TAGTGATCCA 
CCAAATCCTC ATCTTATCAT CCCCACTCCT GAAGGTTTCA GCACGCGGAG TGTCCCTTGT 
GATGCACGGA ACCACTGCAG CGCTGCTGTC AGTCGCCCCG CTCCCATTGG TAGCGACTCA 
GCTCAACCCA AACCTGTGAG CAGTGCAAAT GAGACCAGGG GTTCCAGTGT CCCTGAAAAT 
GATCGACTGG CTTCAATAGC AGCTGAGTTG CAGTTCAGAT CCCTTAGTCG TCACTCCAGT 
CCCACTGAAG AGCGAGAAGA ACCATCTTAT CCAAAAGGAG ATTCAGGTGG ATCATCCCGG 
CGGAGTTGGG AGCTCAGAAC TTTAATCAAC CAGACCAAAG ACACTGCTTC CAAGCAAGCG 
CCAATCGAAG CTGTTCAGAA GTCTGTTCAG TTGTTTGAAG AAAGGAGATA CAGAGAGATG 
AGGAGGAAGA ACATCATTGG CCAAGTTTGT GACACCCCAA AATCTTATGA TAATGTCATG 
CATGTAGGTT TGAGGAAAGT GACTTTCAAG TGGCAGAGAG GAAACAAAAT TGGTGAAGGC 
CAGTACGGAA AAGTCTACAC CTGTATCAGT GTTGACACTG GTGAACTGAT GGCTATGAAA 
GAAATAAGAT TTCAACCCAA TGATCACAAA ACCATCAAAG AAACAGCAGA TGAACTGAAG 
ATTTTTGAAG GCATCAAACA CCCTAATCTT GTTCGTTACT TTGGTGTGGA GCTCCATAGG 
GAAGAAATGT ACATCTTCAT GGAGTACTGT GATGAGGGCA CCCTGGAGGA GGTATCAAAA 
CTGGGCCTTC AGGAACATGT CATTAGGCTC TACTCAAAGC AAATCACAAC TGCTATCAAT 
GTACTGCATG AACATGGTAT TGTTCATCGA GACATTAAAG GAGCCAACAT CTTTCTTACC 
TCATCTGGAC TGATTAAATT AGGAGACTTT GGCTGCTCAG TGAAATTAAA GAATAACACC 
CAGACAATGC CTGGAGAAGT GAACAGTACT TTGGGGACTG CAGCATACAT GGCTCCAGAA 
GTCATTACTA GAGCTAAAGG AGAAGGGCAT GGGCGTGCAG CAGACATCTG GAGCCTGGGC 
TGTGTTGTAA TAGAGATGGT CACTGGCAAG AGGCCGTGGC ATGAATACGA GCACAACTTC 
CAGATCATGT ATAGAGTGGG GATGGGTCAT AAACCTCCTA TTCCTGACAA GGTGAGCCCA 
GAAGGGAAAG ACTTCCTTTG CCACTGTCTG GAAAGTGACC CAAAGATGAG GTGGACGGCT 
AGCCAGCTCC TCGACCATCC CTTTGTCAAG GTTTGCACAG ATGAAGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013800897.1
CDS	140..4870
Translation	MRDVAAVALVSAAAAMEEPPPPGRTECKSEDATCKISESEPEELSDEASADTFYGTSPPSTPRQMKRMSTKHQKNNVGRPASRSTLKEKMGLPTQSVHKDNGKTIENVEEHSYKQGKKNRATLRTTERDHKKNVQCSFMLDSVGGSLPKKTIPDVDLNKPYLSLGCSNAKLPVSVPMPIARTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDREQRGQENTSSVWLNRSNELIWLELQAWHAGRSINDQDLYLYAARQAIPDIINEILTFKVNYGSFSCVKNGASLNGTTGEGVCKATHGTSALGYSSYHEHLHRQRVSFEQVKRIMELLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPGPSKDNDPEDEEVDRETEVKESSGMCTEESDGEEQISEENVEELRQAIKNNFTNKPNFDFQDHFSRKLDRLESEDDSASWVIPECSAEAGCSKHCLTSIYRPFVDKALKQMGLRKLILRLHKLMHGSLQRARMALVRNDHQLEFSEFPDPMLCSDYVQLLNSPPSCEKKCSTVSWEELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLREVLDDLQKIDCNIDSFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITPYIRGGEAQAGKLFCDIAGMLLKSTGIFLDSGLQDSCDEFWASADDSTASDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFALSAPVRDFLNALKTKEYVKVQIPGLENLQVFVPNSIAGEKSVILQLLNAAAGKDCSKDSDEVAYEAYLLMTKHSDKDHELDNSWSAWEGQPVKVVPQVETADTLRSMQVDNLLLVVIQSAHLVSQRKAFQQSIEGLISLHHEQTSSQPVIAKALQQLKSDALQLCNKISSAIDRVDRMFTSEFDAEVDESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYLAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFSTRSVPCDARNHCSAAVSRPAPIGSDSAQPKPVSSANETRGSSVPENDRLASIAAELQFRSLSRHSSPTEEREEPSYPKGDSGGSSRRSWELRTLINQTKDTASKQAPIEAVQKSVQLFEERRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSKLGLQEHVIRLYSKQITTAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNTQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYRVGMGHKPPIPDKVSPEGKDFLCHCLESDPKMRWTASQLLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_013945443.1
Organism	Apteryx australis mantelli
Definition	Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013945443.1

ORF Insert Sequence:

ATGAGAGACG TCGCCGCCGT CGCGCTGGTC TCTGCTGCCG CCGCCATGGA GGAGCCGCCG 
CCGCCGGGGA GGACAGAGTG CAAATCAGAA GATGCTACTT GCAAGATCTC TGAATCTGAA 
CCTGAGGAAT TATCAGATGA AGCAAGTGCT GACACTTTCT ATGGAACTTC TCCTCCTAGT 
ACGCCCCGAC AGATGAAACG TATGTCAACA AAGCATCAGA AAAATAATGT TGGGAGACCT 
GCTAGCCGTT CCACTTTGAA AGAAAAAATG GGTTTACCAA CTCAGTCTGT GCATAAAGAC 
AATGGGAAAA CCATTGAGAA TGTGGAAGAG CACAGCTATA AGCAAGGGAA GAAGAACCGA 
GCCACCCTAA GGACAACAGA ACGAGATCAC AAGAAAAATG TGCAGTGCTC ATTTATGTTA 
GACTCAGTTG GTGGGTCTTT GCCAAAAAAA ACAATTCCAG ATGTTGATCT CAATAAGCCT 
TACCTCAGCC TTGGCTGTAG CAATGCTAAG CTTCCAGTCT CTGTGCCCAT GCCAATAGCC 
AGAACTGCAC GCCAGACTTC TAGGACTGAT TGCCCAGCAG ACCGCTTAAA ATTCTTTGAA 
ACCCTGCGGC TTTTGTTAAA ACTTACCTCA GTCTCGAAGA AAAAGGACAG GGAGCAAAGA 
GGACAGGAAA ACACCTCTTC TGTCTGGCTG AACCGATCCA ATGAACTGAT CTGGCTGGAG 
CTGCAAGCTT GGCATGCTGG CCGGAGCATT AATGACCAAG ACCTATATCT CTATGCGGCC 
CGTCAAGCCA TCCCTGATAT TATCAATGAA ATCCTCACCT TCAAAGTAAA CTATGGAAGC 
TTCTCCTGTG TAAAAAATGG AGCCAGTCTC AATGGTACTA CAGGAGAAGG AGTTTGCAAA 
GCAACACATG GAACTAGTGC TTTGGGTTAC TCAAGTTACC ATGAACACCT CCATCGCCAG 
CGGGTCTCAT TTGAGCAAGT AAAGCGGATA ATGGAGCTGC TAGAGTATAT AGAAGCACTT 
TATCCATCTC TGCAGGCTCT TCAAAAGGAC TATGAAAAGT ATGCTGCAAA GGACTTCCAA 
GACAGAGTGC AGGCACTCTG TCTGTGGTTA AACATTACAA AAGACTTAAA TCAAAAACTA 
AGGATTATGG GAACTGTTTT GGGCATCAAA AATCTTTCTG ACATTGGCTG GCCAGTGTTT 
GAAATTCCTT CTCCTGGACC GTCTAAGGAC AATGATCCAG AGGATGAGGA AGTTGATAGG 
GAAACAGAAG TAAAGGAATC CTCAGGAATG TGCACAGAGG AGAGTGATGG TGAAGAACAA 
ATCTCTGAGG AAAATGTTGA GGAACTTAGA CAAGCCATCA AGAACAATTT TACTAATAAG 
CCAAATTTTG ACTTTCAGGA CCATTTTTCA AGGAAGCTTG ATAGGCTTGA ATCTGAGGAT 
GACTCTGCCT CTTGGGTTAT TCCAGAATGC AGTGCTGAGG CAGGCTGCAG CAAACACTGT 
TTGACCTCTA TTTACAGGCC ATTTGTAGAT AAAGCACTGA AGCAAATGGG GTTGAGGAAG 
CTAATTTTGA GACTGCACAA GCTAATGCAT GGTTCATTGC AAAGGGCCCG TATGGCGTTG 
GTAAGGAACG ATCACCAGCT GGAGTTTTCA GAATTTCCAG ATCCCATGTT GTGTTCAGAT 
TATGTGCAGT TATTAAATTC CCCGCCTTCC TGTGAGAAAA AATGCAGCAC TGTATCATGG 
GAAGAGCTGA AATCTATGGA TTTACCATCT TTTGAACCTG CATTCTTGGT CCTTTGCCGA 
GTCCTGCTCA ATGTTATCCA TGAATGTCTC AAGCTAAGGT TGGAACAAAG ACCTGCTGGG 
GAACCATCTC TTTTGAGTAT TAAGCAGCTA GTAAGAGAGT GTAAAGAGGT CCTGAAGGGC 
GGCCTGTTAA TGAAGCAATA TTATCAGTTC ATGTTACGTG AGGTGTTAGA TGACTTGCAA 
AAGATTGACT GCAACATTGA TTCTTTTGAA GAGGACCTGC ATAAAATGTT AATGGTTTAC 
TTTGATTATA TGAGAAGCTG GATCCAAATG CTACAGCAGT TACCTCAAGC ATCTCATAGT 
TTGAAAAATC TCTTAGAAGA AGAATGGAAT TTCACCAAAG AAATAACTCC TTATATCAGA 
GGAGGTGAAG CTCAAGCTGG AAAACTATTC TGTGATATTG CAGGAATGCT GCTGAAATCT 
ACAGGAATAT TTTTAGACTC TGGACTACAG GACAGTTGTG ATGAGTTTTG GGCCAGTGCT 
GATGATAGTA CTGCATCAGA TGAAATCAGG AGGTCTGTTA TAGAAACCAG CCGAGCACTA 
AAAGAACTCT TTCATGAAGC TAGAGAAAGG GCATCCAAAG CTCTTGGTTT TGCTAAAATG 
CTGAGGAAGG ATCTTGAAAT AGCAGCAGAG TTTGCATTGT CAGCCCCTGT GCGAGATTTT 
CTGAATGCTC TGAAAACAAA AGAGTATGTG AAGGTGCAAA TCCCTGGACT TGAAAACTTA 
CAAGTATTTG TCCCAAATAG TATTGCAGGG GAAAAATCTG TGATTTTACA GCTCCTAAAT 
GCAGCTGCAG GAAAGGATTG CTCGAAGGAT TCGGATGAGG TTGCATATGA GGCCTATTTA 
CTAATGACCA AACACAGTGA CAAAGACCAC GAATTAGACA ACAGTTGGAG CGCGTGGGAG 
GGTCAGCCAG TCAAAGTAGT GCCACAGGTG GAAACTGCTG ATACACTGCG AAGCATGCAG 
GTTGATAACC TCCTCCTTGT AGTCATTCAA TCTGCCCATC TTGTGAGTCA GAGAAAAGCT 
TTCCAACAGT CTATTGAAGG GCTCATATCT TTGCACCACG AACAGACATC CAGCCAACCA 
GTCATTGCCA AAGCTCTGCA ACAGCTAAAG AGTGATGCAT TACAGCTATG CAATAAGATA 
AGCAGTGCCA TTGATAGAGT TGATCGCATG TTCACATCTG AGTTTGATGC TGAAGTTGAT 
GAATCTGAAT CTGCCACTTT GCAACAATAC TACCGGGAAG CCATGATTCA GGGTTATAAT 
TTTGGATTTG AGTACCATAA AGAAGTTGTT CGCCTAATGT CTGGAGAATT CAGGCAGAAA 
ATTGGAGATA AGTATATCAG CTTTGCTAGA AAATGGATGA ATTATGTGCT AACAAAGTGT 
GAGAGTGGCC GAGGCACTCG ACCCAGGTGG GCAACTCAAG GTTTTGATTT TCTTCAAGCC 
ATTGAGCCAG CATTTATTTC AGCTCTCCCA GAAGATGACT TCTTGAGTTT ACAAGCTCTG 
ATGAATGAAT GCATTGGCCA TGTTATTGGA AAACCTCATA GTCCTGTTAC AGGTTTGTAC 
CTTGCTATTC ATCGAAACAG TCCCCGTCCT GTAAAGGTAC CTCGATGCCA TAGTGATCCA 
CCAAATCCTC ATCTTATCAT CCCCACTCCT GAAGGTTTCA GCACGCGGAG TGTCCCTTGT 
GATGCACGGA ACCACTGCAG CGCTGCTGTC AGTCGCCCCG CTCCCATTGG TAGCGACTCA 
GCTCAACCCA AACCTGTGAG CAGTGCAAAT GAGACCAGGG GTTCCAGTGT CCCTGAAAAT 
GATCGACTGG CTTCAATAGC AGCTGAGTTG CAGTTCAGAT CCCTTAGTCG TCACTCCAGT 
CCCACTGAAG AGCGAGAAGA ACCATCTTAT CCAAAAGGAG ATTCAGGTGG ATCATCCCGG 
CGGAGTTGGG AGCTCAGAAC TTTAATCAAC CAGACCAAAG ACACTGCTTC CAAGCAAGCG 
CCAATCGAAG CTGTTCAGAA GTCTGTTCAG TTGTTTGAAG AAAGGAGATA CAGAGAGATG 
AGGAGGAAGA ACATCATTGG CCAAGTTTGT GACACCCCAA AATCTTATGA TAATGTCATG 
CATGTAGGTT TGAGGAAAGT GACTTTCAAG TGGCAGAGAG GAAACAAAAT TGGTGAAGGC 
CAGTACGGAA AAGTCTACAC CTGTATCAGT GTTGACACTG GTGAACTGAT GGCTATGAAA 
GAAATAAGAT TTCAACCCAA TGATCACAAA ACCATCAAAG AAACAGCAGA TGAACTGAAG 
ATTTTTGAAG GCATCAAACA CCCTAATCTT GTTCGTTACT TTGGTGTGGA GCTCCATAGG 
GAAGAAATGT ACATCTTCAT GGAGTACTGT GATGAGGGCA CCCTGGAGGA GGTATCAAAA 
CTGGGCCTTC AGGAACATGT CATTAGGCTC TACTCAAAGC AAATCACAAC TGCTATCAAT 
GTACTGCATG AACATGGTAT TGTTCATCGA GACATTAAAG GAGCCAACAT CTTTCTTACC 
TCATCTGGAC TGATTAAATT AGGAGACTTT GGCTGCTCAG TGAAATTAAA GAATAACACC 
CAGACAATGC CTGGAGAAGT GAACAGTACT TTGGGGACTG CAGCATACAT GGCTCCAGAA 
GTCATTACTA GAGCTAAAGG AGAAGGGCAT GGGCGTGCAG CAGACATCTG GAGCCTGGGC 
TGTGTTGTAA TAGAGATGGT CACTGGCAAG AGGCCGTGGC ATGAATACGA GCACAACTTC 
CAGATCATGT ATAGAGTGGG GATGGGTCAT AAACCTCCTA TTCCTGACAA GGTGAGCCCA 
GAAGGGAAAG ACTTCCTTTG CCACTGTCTG GAAAGTGACC CAAAGATGAG GTGGACGGCT 
AGCCAGCTCC TCGACCATCC CTTTGTCAAG GTTTGCACAG ATGAAGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_013945444.1 ) cDNA ORF clone, Apteryx australis mantelli -> XP_013800898.1 Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OAp16193
Clone ID Related Accession (Same CDS sequence)	XM_013945444.1
Accession Version	XM_013945444.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-08
Organism	Apteryx australis mantelli
Product	mitogen-activated protein kinase kinase kinase 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013993955.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apteryx australis mantelli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGACG TCGCCGCCGT CGCGCTGGTC TCTGCTGCCG CCGCCATGGA GGAGCCGCCG 
CCGCCGGGGA GGACAGAGTG CAAATCAGAA GATGCTACTT GCAAGATCTC TGAATCTGAA 
CCTGAGGAAT TATCAGATGA AGCAAGTGCT GACACTTTCT ATGGAACTTC TCCTCCTAGT 
ACGCCCCGAC AGATGAAACG TATGTCAACA AAGCATCAGA AAAATAATGT TGGGAGACCT 
GCTAGCCGTT CCACTTTGAA AGAAAAAATG GGTTTACCAA CTCAGTCTGT GCATAAAGAC 
AATGGGAAAA CCATTGAGAA TGTGGAAGAG CACAGCTATA AGCAAGGGAA GAAGAACCGA 
GCCACCCTAA GGACAACAGA ACGAGATCAC AAGAAAAATG TGCAGTGCTC ATTTATGTTA 
GACTCAGTTG GTGGGTCTTT GCCAAAAAAA ACAATTCCAG ATGTTGATCT CAATAAGCCT 
TACCTCAGCC TTGGCTGTAG CAATGCTAAG CTTCCAGTCT CTGTGCCCAT GCCAATAGCC 
AGAACTGCAC GCCAGACTTC TAGGACTGAT TGCCCAGCAG ACCGCTTAAA ATTCTTTGAA 
ACCCTGCGGC TTTTGTTAAA ACTTACCTCA GTCTCGAAGA AAAAGGACAG GGAGCAAAGA 
GGACAGGAAA ACACCTCTTC TGTCTGGCTG AACCGATCCA ATGAACTGAT CTGGCTGGAG 
CTGCAAGCTT GGCATGCTGG CCGGAGCATT AATGACCAAG ACCTATATCT CTATGCGGCC 
CGTCAAGCCA TCCCTGATAT TATCAATGAA ATCCTCACCT TCAAAGTAAA CTATGGAAGC 
TTCTCCTGTG TAAAAAATGG AGCCAGTCTC AATGGTACTA CAGGAGAAGG AGTTTGCAAA 
GCAACACATG GAACTAGTGC TTTGGGTTAC TCAAGTTACC ATGAACACCT CCATCGCCAG 
CGGGTCTCAT TTGAGCAAGT AAAGCGGATA ATGGAGCTGC TAGAGTATAT AGAAGCACTT 
TATCCATCTC TGCAGGCTCT TCAAAAGGAC TATGAAAAGT ATGCTGCAAA GGACTTCCAA 
GACAGAGTGC AGGCACTCTG TCTGTGGTTA AACATTACAA AAGACTTAAA TCAAAAACTA 
AGGATTATGG GAACTGTTTT GGGCATCAAA AATCTTTCTG ACATTGGCTG GCCAGTGTTT 
GAAATTCCTT CTCCTGGACC GTCTAAGGAC AATGATCCAG AGGATGAGGA AGTTGATAGG 
GAAACAGAAG TAAAGGAATC CTCAGGAATG TGCACAGAGG AGAGTGATGG TGAAGAACAA 
ATCTCTGAGG AAAATGTTGA GGAACTTAGA CAAGCCATCA AGAACAATTT TACTAATAAG 
CCAAATTTTG ACTTTCAGGA CCATTTTTCA AGGAAGCTTG ATAGGCTTGA ATCTGAGGAT 
GACTCTGCCT CTTGGGTTAT TCCAGAATGC AGTGCTGAGG CAGGCTGCAG CAAACACTGT 
TTGACCTCTA TTTACAGGCC ATTTGTAGAT AAAGCACTGA AGCAAATGGG GTTGAGGAAG 
CTAATTTTGA GACTGCACAA GCTAATGCAT GGTTCATTGC AAAGGGCCCG TATGGCGTTG 
GTAAGGAACG ATCACCAGCT GGAGTTTTCA GAATTTCCAG ATCCCATGTT GTGTTCAGAT 
TATGTGCAGT TATTAAATTC CCCGCCTTCC TGTGAGAAAA AATGCAGCAC TGTATCATGG 
GAAGAGCTGA AATCTATGGA TTTACCATCT TTTGAACCTG CATTCTTGGT CCTTTGCCGA 
GTCCTGCTCA ATGTTATCCA TGAATGTCTC AAGCTAAGGT TGGAACAAAG ACCTGCTGGG 
GAACCATCTC TTTTGAGTAT TAAGCAGCTA GTAAGAGAGT GTAAAGAGGT CCTGAAGGGC 
GGCCTGTTAA TGAAGCAATA TTATCAGTTC ATGTTACGTG AGGTGTTAGA TGACTTGCAA 
AAGATTGACT GCAACATTGA TTCTTTTGAA GAGGACCTGC ATAAAATGTT AATGGTTTAC 
TTTGATTATA TGAGAAGCTG GATCCAAATG CTACAGCAGT TACCTCAAGC ATCTCATAGT 
TTGAAAAATC TCTTAGAAGA AGAATGGAAT TTCACCAAAG AAATAACTCC TTATATCAGA 
GGAGGTGAAG CTCAAGCTGG AAAACTATTC TGTGATATTG CAGGAATGCT GCTGAAATCT 
ACAGGAATAT TTTTAGACTC TGGACTACAG GACAGTTGTG ATGAGTTTTG GGCCAGTGCT 
GATGATAGTA CTGCATCAGA TGAAATCAGG AGGTCTGTTA TAGAAACCAG CCGAGCACTA 
AAAGAACTCT TTCATGAAGC TAGAGAAAGG GCATCCAAAG CTCTTGGTTT TGCTAAAATG 
CTGAGGAAGG ATCTTGAAAT AGCAGCAGAG TTTGCATTGT CAGCCCCTGT GCGAGATTTT 
CTGAATGCTC TGAAAACAAA AGAGTATGTG AAGGTGCAAA TCCCTGGACT TGAAAACTTA 
CAAGTATTTG TCCCAAATAG TATTGCAGGG GAAAAATCTG TGATTTTACA GCTCCTAAAT 
GCAGCTGCAG GAAAGGATTG CTCGAAGGAT TCGGATGAGG TTGCATATGA GGCCTATTTA 
CTAATGACCA AACACAGTGA CAAAGACCAC GAATTAGACA ACAGTTGGAG CGCGTGGGAG 
GGTCAGCCAG TCAAAGTAGT GCCACAGGTG GAAACTGCTG ATACACTGCG AAGCATGCAG 
GTTGATAACC TCCTCCTTGT AGTCATTCAA TCTGCCCATC TTGTGAGTCA GAGAAAAGCT 
TTCCAACAGT CTATTGAAGG GCTCATATCT TTGCACCACG AACAGACATC CAGCCAACCA 
GTCATTGCCA AAGCTCTGCA ACAGCTAAAG AGTGATGCAT TACAGCTATG CAATAAGATA 
AGCAGTGCCA TTGATAGAGT TGATCGCATG TTCACATCTG AGTTTGATGC TGAAGTTGAT 
GAATCTGAAT CTGCCACTTT GCAACAATAC TACCGGGAAG CCATGATTCA GGGTTATAAT 
TTTGGATTTG AGTACCATAA AGAAGTTGTT CGCCTAATGT CTGGAGAATT CAGGCAGAAA 
ATTGGAGATA AGTATATCAG CTTTGCTAGA AAATGGATGA ATTATGTGCT AACAAAGTGT 
GAGAGTGGCC GAGGCACTCG ACCCAGGTGG GCAACTCAAG GTTTTGATTT TCTTCAAGCC 
ATTGAGCCAG CATTTATTTC AGCTCTCCCA GAAGATGACT TCTTGAGTTT ACAAGCTCTG 
ATGAATGAAT GCATTGGCCA TGTTATTGGA AAACCTCATA GTCCTGTTAC AGCTATTCAT 
CGAAACAGTC CCCGTCCTGT AAAGGTACCT CGATGCCATA GTGATCCACC AAATCCTCAT 
CTTATCATCC CCACTCCTGA AGGTTTCAGC ACGCGGAGTG TCCCTTGTGA TGCACGGAAC 
CACTGCAGCG CTGCTGTCAG TCGCCCCGCT CCCATTGGTA GCGACTCAGC TCAACCCAAA 
CCTGTGAGCA GTGCAAATGA GACCAGGGGT TCCAGTGTCC CTGAAAATGA TCGACTGGCT 
TCAATAGCAG CTGAGTTGCA GTTCAGATCC CTTAGTCGTC ACTCCAGTCC CACTGAAGAG 
CGAGAAGAAC CATCTTATCC AAAAGGAGAT TCAGGTGGAT CATCCCGGCG GAGTTGGGAG 
CTCAGAACTT TAATCAACCA GACCAAAGAC ACTGCTTCCA AGCAAGCGCC AATCGAAGCT 
GTTCAGAAGT CTGTTCAGTT GTTTGAAGAA AGGAGATACA GAGAGATGAG GAGGAAGAAC 
ATCATTGGCC AAGTTTGTGA CACCCCAAAA TCTTATGATA ATGTCATGCA TGTAGGTTTG 
AGGAAAGTGA CTTTCAAGTG GCAGAGAGGA AACAAAATTG GTGAAGGCCA GTACGGAAAA 
GTCTACACCT GTATCAGTGT TGACACTGGT GAACTGATGG CTATGAAAGA AATAAGATTT 
CAACCCAATG ATCACAAAAC CATCAAAGAA ACAGCAGATG AACTGAAGAT TTTTGAAGGC 
ATCAAACACC CTAATCTTGT TCGTTACTTT GGTGTGGAGC TCCATAGGGA AGAAATGTAC 
ATCTTCATGG AGTACTGTGA TGAGGGCACC CTGGAGGAGG TATCAAAACT GGGCCTTCAG 
GAACATGTCA TTAGGCTCTA CTCAAAGCAA ATCACAACTG CTATCAATGT ACTGCATGAA 
CATGGTATTG TTCATCGAGA CATTAAAGGA GCCAACATCT TTCTTACCTC ATCTGGACTG 
ATTAAATTAG GAGACTTTGG CTGCTCAGTG AAATTAAAGA ATAACACCCA GACAATGCCT 
GGAGAAGTGA ACAGTACTTT GGGGACTGCA GCATACATGG CTCCAGAAGT CATTACTAGA 
GCTAAAGGAG AAGGGCATGG GCGTGCAGCA GACATCTGGA GCCTGGGCTG TGTTGTAATA 
GAGATGGTCA CTGGCAAGAG GCCGTGGCAT GAATACGAGC ACAACTTCCA GATCATGTAT 
AGAGTGGGGA TGGGTCATAA ACCTCCTATT CCTGACAAGG TGAGCCCAGA AGGGAAAGAC 
TTCCTTTGCC ACTGTCTGGA AAGTGACCCA AAGATGAGGT GGACGGCTAG CCAGCTCCTC 
GACCATCCCT TTGTCAAGGT TTGCACAGAT GAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013800898.1
CDS	140..4858
Translation	MRDVAAVALVSAAAAMEEPPPPGRTECKSEDATCKISESEPEELSDEASADTFYGTSPPSTPRQMKRMSTKHQKNNVGRPASRSTLKEKMGLPTQSVHKDNGKTIENVEEHSYKQGKKNRATLRTTERDHKKNVQCSFMLDSVGGSLPKKTIPDVDLNKPYLSLGCSNAKLPVSVPMPIARTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDREQRGQENTSSVWLNRSNELIWLELQAWHAGRSINDQDLYLYAARQAIPDIINEILTFKVNYGSFSCVKNGASLNGTTGEGVCKATHGTSALGYSSYHEHLHRQRVSFEQVKRIMELLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPGPSKDNDPEDEEVDRETEVKESSGMCTEESDGEEQISEENVEELRQAIKNNFTNKPNFDFQDHFSRKLDRLESEDDSASWVIPECSAEAGCSKHCLTSIYRPFVDKALKQMGLRKLILRLHKLMHGSLQRARMALVRNDHQLEFSEFPDPMLCSDYVQLLNSPPSCEKKCSTVSWEELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLREVLDDLQKIDCNIDSFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITPYIRGGEAQAGKLFCDIAGMLLKSTGIFLDSGLQDSCDEFWASADDSTASDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFALSAPVRDFLNALKTKEYVKVQIPGLENLQVFVPNSIAGEKSVILQLLNAAAGKDCSKDSDEVAYEAYLLMTKHSDKDHELDNSWSAWEGQPVKVVPQVETADTLRSMQVDNLLLVVIQSAHLVSQRKAFQQSIEGLISLHHEQTSSQPVIAKALQQLKSDALQLCNKISSAIDRVDRMFTSEFDAEVDESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFSTRSVPCDARNHCSAAVSRPAPIGSDSAQPKPVSSANETRGSSVPENDRLASIAAELQFRSLSRHSSPTEEREEPSYPKGDSGGSSRRSWELRTLINQTKDTASKQAPIEAVQKSVQLFEERRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSKLGLQEHVIRLYSKQITTAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNTQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYRVGMGHKPPIPDKVSPEGKDFLCHCLESDPKMRWTASQLLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_013945444.1
Organism	Apteryx australis mantelli
Definition	Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013945444.1

ORF Insert Sequence:

ATGAGAGACG TCGCCGCCGT CGCGCTGGTC TCTGCTGCCG CCGCCATGGA GGAGCCGCCG 
CCGCCGGGGA GGACAGAGTG CAAATCAGAA GATGCTACTT GCAAGATCTC TGAATCTGAA 
CCTGAGGAAT TATCAGATGA AGCAAGTGCT GACACTTTCT ATGGAACTTC TCCTCCTAGT 
ACGCCCCGAC AGATGAAACG TATGTCAACA AAGCATCAGA AAAATAATGT TGGGAGACCT 
GCTAGCCGTT CCACTTTGAA AGAAAAAATG GGTTTACCAA CTCAGTCTGT GCATAAAGAC 
AATGGGAAAA CCATTGAGAA TGTGGAAGAG CACAGCTATA AGCAAGGGAA GAAGAACCGA 
GCCACCCTAA GGACAACAGA ACGAGATCAC AAGAAAAATG TGCAGTGCTC ATTTATGTTA 
GACTCAGTTG GTGGGTCTTT GCCAAAAAAA ACAATTCCAG ATGTTGATCT CAATAAGCCT 
TACCTCAGCC TTGGCTGTAG CAATGCTAAG CTTCCAGTCT CTGTGCCCAT GCCAATAGCC 
AGAACTGCAC GCCAGACTTC TAGGACTGAT TGCCCAGCAG ACCGCTTAAA ATTCTTTGAA 
ACCCTGCGGC TTTTGTTAAA ACTTACCTCA GTCTCGAAGA AAAAGGACAG GGAGCAAAGA 
GGACAGGAAA ACACCTCTTC TGTCTGGCTG AACCGATCCA ATGAACTGAT CTGGCTGGAG 
CTGCAAGCTT GGCATGCTGG CCGGAGCATT AATGACCAAG ACCTATATCT CTATGCGGCC 
CGTCAAGCCA TCCCTGATAT TATCAATGAA ATCCTCACCT TCAAAGTAAA CTATGGAAGC 
TTCTCCTGTG TAAAAAATGG AGCCAGTCTC AATGGTACTA CAGGAGAAGG AGTTTGCAAA 
GCAACACATG GAACTAGTGC TTTGGGTTAC TCAAGTTACC ATGAACACCT CCATCGCCAG 
CGGGTCTCAT TTGAGCAAGT AAAGCGGATA ATGGAGCTGC TAGAGTATAT AGAAGCACTT 
TATCCATCTC TGCAGGCTCT TCAAAAGGAC TATGAAAAGT ATGCTGCAAA GGACTTCCAA 
GACAGAGTGC AGGCACTCTG TCTGTGGTTA AACATTACAA AAGACTTAAA TCAAAAACTA 
AGGATTATGG GAACTGTTTT GGGCATCAAA AATCTTTCTG ACATTGGCTG GCCAGTGTTT 
GAAATTCCTT CTCCTGGACC GTCTAAGGAC AATGATCCAG AGGATGAGGA AGTTGATAGG 
GAAACAGAAG TAAAGGAATC CTCAGGAATG TGCACAGAGG AGAGTGATGG TGAAGAACAA 
ATCTCTGAGG AAAATGTTGA GGAACTTAGA CAAGCCATCA AGAACAATTT TACTAATAAG 
CCAAATTTTG ACTTTCAGGA CCATTTTTCA AGGAAGCTTG ATAGGCTTGA ATCTGAGGAT 
GACTCTGCCT CTTGGGTTAT TCCAGAATGC AGTGCTGAGG CAGGCTGCAG CAAACACTGT 
TTGACCTCTA TTTACAGGCC ATTTGTAGAT AAAGCACTGA AGCAAATGGG GTTGAGGAAG 
CTAATTTTGA GACTGCACAA GCTAATGCAT GGTTCATTGC AAAGGGCCCG TATGGCGTTG 
GTAAGGAACG ATCACCAGCT GGAGTTTTCA GAATTTCCAG ATCCCATGTT GTGTTCAGAT 
TATGTGCAGT TATTAAATTC CCCGCCTTCC TGTGAGAAAA AATGCAGCAC TGTATCATGG 
GAAGAGCTGA AATCTATGGA TTTACCATCT TTTGAACCTG CATTCTTGGT CCTTTGCCGA 
GTCCTGCTCA ATGTTATCCA TGAATGTCTC AAGCTAAGGT TGGAACAAAG ACCTGCTGGG 
GAACCATCTC TTTTGAGTAT TAAGCAGCTA GTAAGAGAGT GTAAAGAGGT CCTGAAGGGC 
GGCCTGTTAA TGAAGCAATA TTATCAGTTC ATGTTACGTG AGGTGTTAGA TGACTTGCAA 
AAGATTGACT GCAACATTGA TTCTTTTGAA GAGGACCTGC ATAAAATGTT AATGGTTTAC 
TTTGATTATA TGAGAAGCTG GATCCAAATG CTACAGCAGT TACCTCAAGC ATCTCATAGT 
TTGAAAAATC TCTTAGAAGA AGAATGGAAT TTCACCAAAG AAATAACTCC TTATATCAGA 
GGAGGTGAAG CTCAAGCTGG AAAACTATTC TGTGATATTG CAGGAATGCT GCTGAAATCT 
ACAGGAATAT TTTTAGACTC TGGACTACAG GACAGTTGTG ATGAGTTTTG GGCCAGTGCT 
GATGATAGTA CTGCATCAGA TGAAATCAGG AGGTCTGTTA TAGAAACCAG CCGAGCACTA 
AAAGAACTCT TTCATGAAGC TAGAGAAAGG GCATCCAAAG CTCTTGGTTT TGCTAAAATG 
CTGAGGAAGG ATCTTGAAAT AGCAGCAGAG TTTGCATTGT CAGCCCCTGT GCGAGATTTT 
CTGAATGCTC TGAAAACAAA AGAGTATGTG AAGGTGCAAA TCCCTGGACT TGAAAACTTA 
CAAGTATTTG TCCCAAATAG TATTGCAGGG GAAAAATCTG TGATTTTACA GCTCCTAAAT 
GCAGCTGCAG GAAAGGATTG CTCGAAGGAT TCGGATGAGG TTGCATATGA GGCCTATTTA 
CTAATGACCA AACACAGTGA CAAAGACCAC GAATTAGACA ACAGTTGGAG CGCGTGGGAG 
GGTCAGCCAG TCAAAGTAGT GCCACAGGTG GAAACTGCTG ATACACTGCG AAGCATGCAG 
GTTGATAACC TCCTCCTTGT AGTCATTCAA TCTGCCCATC TTGTGAGTCA GAGAAAAGCT 
TTCCAACAGT CTATTGAAGG GCTCATATCT TTGCACCACG AACAGACATC CAGCCAACCA 
GTCATTGCCA AAGCTCTGCA ACAGCTAAAG AGTGATGCAT TACAGCTATG CAATAAGATA 
AGCAGTGCCA TTGATAGAGT TGATCGCATG TTCACATCTG AGTTTGATGC TGAAGTTGAT 
GAATCTGAAT CTGCCACTTT GCAACAATAC TACCGGGAAG CCATGATTCA GGGTTATAAT 
TTTGGATTTG AGTACCATAA AGAAGTTGTT CGCCTAATGT CTGGAGAATT CAGGCAGAAA 
ATTGGAGATA AGTATATCAG CTTTGCTAGA AAATGGATGA ATTATGTGCT AACAAAGTGT 
GAGAGTGGCC GAGGCACTCG ACCCAGGTGG GCAACTCAAG GTTTTGATTT TCTTCAAGCC 
ATTGAGCCAG CATTTATTTC AGCTCTCCCA GAAGATGACT TCTTGAGTTT ACAAGCTCTG 
ATGAATGAAT GCATTGGCCA TGTTATTGGA AAACCTCATA GTCCTGTTAC AGCTATTCAT 
CGAAACAGTC CCCGTCCTGT AAAGGTACCT CGATGCCATA GTGATCCACC AAATCCTCAT 
CTTATCATCC CCACTCCTGA AGGTTTCAGC ACGCGGAGTG TCCCTTGTGA TGCACGGAAC 
CACTGCAGCG CTGCTGTCAG TCGCCCCGCT CCCATTGGTA GCGACTCAGC TCAACCCAAA 
CCTGTGAGCA GTGCAAATGA GACCAGGGGT TCCAGTGTCC CTGAAAATGA TCGACTGGCT 
TCAATAGCAG CTGAGTTGCA GTTCAGATCC CTTAGTCGTC ACTCCAGTCC CACTGAAGAG 
CGAGAAGAAC CATCTTATCC AAAAGGAGAT TCAGGTGGAT CATCCCGGCG GAGTTGGGAG 
CTCAGAACTT TAATCAACCA GACCAAAGAC ACTGCTTCCA AGCAAGCGCC AATCGAAGCT 
GTTCAGAAGT CTGTTCAGTT GTTTGAAGAA AGGAGATACA GAGAGATGAG GAGGAAGAAC 
ATCATTGGCC AAGTTTGTGA CACCCCAAAA TCTTATGATA ATGTCATGCA TGTAGGTTTG 
AGGAAAGTGA CTTTCAAGTG GCAGAGAGGA AACAAAATTG GTGAAGGCCA GTACGGAAAA 
GTCTACACCT GTATCAGTGT TGACACTGGT GAACTGATGG CTATGAAAGA AATAAGATTT 
CAACCCAATG ATCACAAAAC CATCAAAGAA ACAGCAGATG AACTGAAGAT TTTTGAAGGC 
ATCAAACACC CTAATCTTGT TCGTTACTTT GGTGTGGAGC TCCATAGGGA AGAAATGTAC 
ATCTTCATGG AGTACTGTGA TGAGGGCACC CTGGAGGAGG TATCAAAACT GGGCCTTCAG 
GAACATGTCA TTAGGCTCTA CTCAAAGCAA ATCACAACTG CTATCAATGT ACTGCATGAA 
CATGGTATTG TTCATCGAGA CATTAAAGGA GCCAACATCT TTCTTACCTC ATCTGGACTG 
ATTAAATTAG GAGACTTTGG CTGCTCAGTG AAATTAAAGA ATAACACCCA GACAATGCCT 
GGAGAAGTGA ACAGTACTTT GGGGACTGCA GCATACATGG CTCCAGAAGT CATTACTAGA 
GCTAAAGGAG AAGGGCATGG GCGTGCAGCA GACATCTGGA GCCTGGGCTG TGTTGTAATA 
GAGATGGTCA CTGGCAAGAG GCCGTGGCAT GAATACGAGC ACAACTTCCA GATCATGTAT 
AGAGTGGGGA TGGGTCATAA ACCTCCTATT CCTGACAAGG TGAGCCCAGA AGGGAAAGAC 
TTCCTTTGCC ACTGTCTGGA AAGTGACCCA AAGATGAGGT GGACGGCTAG CCAGCTCCTC 
GACCATCCCT TTGTCAAGGT TTGCACAGAT GAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_013945445.1 ) cDNA ORF clone, Apteryx australis mantelli -> XP_013800899.1 Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OAp16194
Clone ID Related Accession (Same CDS sequence)	XM_013945445.1
Accession Version	XM_013945445.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-08
Organism	Apteryx australis mantelli
Product	mitogen-activated protein kinase kinase kinase 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013993955.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apteryx australis mantelli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGACG TCGCCGCCGT CGCGCTGGTC TCTGCTGCCG CCGCCATGGA GGAGCCGCCG 
CCGCCGGGGA GGACAGAGTG CAAATCAGAA GATGCTACTT GCAAGATCTC TGAATCTGAA 
CCTGAGGAAT TATCAGATGA AGCAAGTGCT GACACTTTCT ATGGAACTTC TCCTCCTAGT 
ACGCCCCGAC AGATGAAACG TATGTCAACA AAGCATCAGA AAAATAATGT TGGGAGACCT 
GCTAGCCGTT CCACTTTGAA AGAAAAAATG GGTTTACCAA CTCAGTCTGT GCATAAAGAC 
AATGGGAAAA CCATTGAGAA TGTGGAAGAG CACAGCTATA AGCAAGGGAA GAAGAACCGA 
GCCACCCTAA GGACAACAGA ACGAGATCAC AAGAAAAATG TGCAGTGCTC ATTTATGTTA 
GACTCAGTTG GTGGGTCTTT GCCAAAAAAA ACAATTCCAG ATGTTGATCT CAATAAGCCT 
TACCTCAGCC TTGGCTGTAG CAATGCTAAG CTTCCAGTCT CTGTGCCCAT GCCAATAGCC 
AGAACTGCAC GCCAGACTTC TAGGACTGAT TGCCCAGCAG ACCGCTTAAA ATTCTTTGAA 
ACCCTGCGGC TTTTGTTAAA ACTTACCTCA GTCTCGAAGA AAAAGGACAG GGAGCAAAGA 
GGACAGGAAA ACACCTCTTC TGTCTGGCTG AACCGATCCA ATGAACTGAT CTGGCTGGAG 
CTGCAAGCTT GGCATGCTGG CCGGAGCATT AATGACCAAG ACCTATATCT CTATGCGGCC 
CGTCAAGCCA TCCCTGATAT TATCAATGAA ATCCTCACCT TCAAAGTAAA CTATGGAAGC 
TTCTCCTGTG TAAAAAATGG AGCCAGTCTC AATGGTACTA CAGGAGAAGG AGTTTGCAAA 
GCAACACATG GAACTAGTGC TTTGGGTTAC TCAAGTTACC ATGAACACCT CCATCGCCAG 
CGGGTCTCAT TTGAGCAAGT AAAGCGGATA ATGGAGCTGC TAGAGTATAT AGAAGCACTT 
TATCCATCTC TGCAGGCTCT TCAAAAGGAC TATGAAAAGT ATGCTGCAAA GGACTTCCAA 
GACAGAGTGC AGGCACTCTG TCTGTGGTTA AACATTACAA AAGACTTAAA TCAAAAACTA 
AGGATTATGG GAACTGTTTT GGGCATCAAA AATCTTTCTG ACATTGGCTG GCCAGTGTTT 
GAAATTCCTT CTCCTGGACC GTCTAAGGAC AATGATCCAG AGGATGAGGA AGTTGATAGG 
GAAACAGAAG TAAAGGAATC CTCAGGAATG TGCACAGAGG AGAGTGATGG TGAAGAACAA 
ATCTCTGAGG AAAATGTTGA GGAACTTAGA CAAGCCATCA AGAACAATTT TACTAATAAG 
CCAAATTTTG ACTTTCAGGA CCATTTTTCA AGGAAGCTTG ATAGGCTTGA ATCTGAGGAT 
GACTCTGCCT CTTGGGTTAT TCCAGAATGC AGTGCTGAGG CAGGCTGCAG CAAACACTGT 
TTGACCTCTA TTTACAGGCC ATTTGTAGAT AAAGCACTGA AGCAAATGGG GTTGAGGAAG 
CTAATTTTGA GACTGCACAA GCTAATGCAT GGTTCATTGC AAAGGGCCCG TATGGCGTTG 
GTAAGGAACG ATCACCAGCT GGAGTTTTCA GAATTTCCAG ATCCCATGTT GTGTTCAGAT 
TATGTGCAGT TATTAAATTC CCCGCCTTCC TGTGAGAAAA AATGCAGCAC TGTATCATGG 
GAAGAGCTGA AATCTATGGA TTTACCATCT TTTGAACCTG CATTCTTGGT CCTTTGCCGA 
GTCCTGCTCA ATGTTATCCA TGAATGTCTC AAGCTAAGGT TGGAACAAAG ACCTGCTGGG 
GAACCATCTC TTTTGAGTAT TAAGCAGCTA GTAAGAGAGT GTAAAGAGGT CCTGAAGGGC 
GGCCTGTTAA TGAAGCAATA TTATCAGTTC ATGTTACGTG AGGTGTTAGA TGACTTGCAA 
AAGATTGACT GCAACATTGA TTCTTTTGAA GAGGACCTGC ATAAAATGTT AATGGTTTAC 
TTTGATTATA TGAGAAGCTG GATCCAAATG CTACAGCAGT TACCTCAAGC ATCTCATAGT 
TTGAAAAATC TCTTAGAAGA AGAATGGAAT TTCACCAAAG AAATAACTCC TTATATCAGA 
GGAGGTGAAG CTCAAGCTGG AAAACTATTC TGTGATATTG CAGGAATGCT GCTGAAATCT 
ACAGGAATAT TTTTAGACTC TGGACTACAG GACAGTTGTG ATGAGTTTTG GGCCAGTGCT 
GATGATAGTA CTGCATCAGA TGAAATCAGG AGGTCTGTTA TAGAAACCAG CCGAGCACTA 
AAAGAACTCT TTCATGAAGC TAGAGAAAGG GCATCCAAAG CTCTTGGTTT TGCTAAAATG 
CTGAGGAAGG ATCTTGAAAT AGCAGCAGAG TTTGCATTGT CAGCCCCTGT GCGAGATTTT 
CTGAATGCTC TGAAAACAAA AGAGTATGTG AAGGTGCAAA TCCCTGGACT TGAAAACTTA 
CAAGTATTTG TCCCAAATAG TATTGCAGGG GAAAAATCTG TGATTTTACA GCTCCTAAAT 
GCAGCTGCAG GAAAGGATTG CTCGAAGGAT TCGGATGAGG TTGCATATGA GGCCTATTTA 
CTAATGACCA AACACAGTGA CAAAGACCAC GAATTAGACA ACAGTTGGAG CGCGTGGGAG 
GGTCAGCCAG TCAAAGTAGT GCCACAGGTG GAAACTGCTG ATACACTGCG AAGCATGCAG 
GTTGATAACC TCCTCCTTGT AGTCATTCAA TCTGCCCATC TTGTGAGTCA GAGAAAAGCT 
TTCCAACAGT CTATTGAAGG GCTCATATCT TTGCACCACG AACAGACATC CAGCCAACCA 
GTCATTGCCA AAGCTCTGCA ACAGCTAAAG AGTGATGCAT TACAGCTATG CAATAAGATA 
AGCAGTGCCA TTGATAGAGT TGATCGCATG TTCACATCTG AGTTTGATGC TGAAGTTGAT 
GAATCTGAAT CTGCCACTTT GCAACAATAC TACCGGGAAG CCATGATTCA GGGTTATAAT 
TTTGGATTTG AGTACCATAA AGAAGTTGTT CGCCTAATGT CTGGAGAATT CAGGCAGAAA 
ATTGGAGATA AGTATATCAG CTTTGCTAGA AAATGGATGA ATTATGTGCT AACAAAGTGT 
GAGAGTGGCC GAGGCACTCG ACCCAGGTGG GCAACTCAAG GTTTTGATTT TCTTCAAGCC 
ATTGAGCCAG CATTTATTTC AGCTCTCCCA GAAGATGACT TCTTGAGTTT ACAAGCTCTG 
ATGAATGAAT GCATTGGCCA TGTTATTGGA AAACCTCATA GTCCTGTTAC AGCTATTCAT 
CGAAACAGTC CCCGTCCTGT AAAGGTACCT CGATGCCATA GTGATCCACC AAATCCTCAT 
CTTATCATCC CCACTCCTGA AGGTTTCAGT TTTTCTTCTT GCCACAGCAC GCGGAGTGTC 
CCTTGTGATG CACGGAACCA CTGCAGCGCT GCTGTCAGTC GCCCCGCTCC CATTGGTAGC 
GACTCAGCTC AACCCAAACC TGTGAGCAGT GCAAATGAGA CCAGGGGTTC CAGTGTCCCT 
GAAAATGATC GACTGGCTTC AATAGCAGCT GAGTTGCAGT TCAGATCCCT TAGTCGTCAC 
TCCAGTCCCA CTGAAGAGCG AGAAGACACT GCTTCCAAGC AAGCGCCAAT CGAAGCTGTT 
CAGAAGTCTG TTCAGTTGTT TGAAGAAAGG AGATACAGAG AGATGAGGAG GAAGAACATC 
ATTGGCCAAG TTTGTGACAC CCCAAAATCT TATGATAATG TCATGCATGT AGGTTTGAGG 
AAAGTGACTT TCAAGTGGCA GAGAGGAAAC AAAATTGGTG AAGGCCAGTA CGGAAAAGTC 
TACACCTGTA TCAGTGTTGA CACTGGTGAA CTGATGGCTA TGAAAGAAAT AAGATTTCAA 
CCCAATGATC ACAAAACCAT CAAAGAAACA GCAGATGAAC TGAAGATTTT TGAAGGCATC 
AAACACCCTA ATCTTGTTCG TTACTTTGGT GTGGAGCTCC ATAGGGAAGA AATGTACATC 
TTCATGGAGT ACTGTGATGA GGGCACCCTG GAGGAGGTAT CAAAACTGGG CCTTCAGGAA 
CATGTCATTA GGCTCTACTC AAAGCAAATC ACAACTGCTA TCAATGTACT GCATGAACAT 
GGTATTGTTC ATCGAGACAT TAAAGGAGCC AACATCTTTC TTACCTCATC TGGACTGATT 
AAATTAGGAG ACTTTGGCTG CTCAGTGAAA TTAAAGAATA ACACCCAGAC AATGCCTGGA 
GAAGTGAACA GTACTTTGGG GACTGCAGCA TACATGGCTC CAGAAGTCAT TACTAGAGCT 
AAAGGAGAAG GGCATGGGCG TGCAGCAGAC ATCTGGAGCC TGGGCTGTGT TGTAATAGAG 
ATGGTCACTG GCAAGAGGCC GTGGCATGAA TACGAGCACA ACTTCCAGAT CATGTATAGA 
GTGGGGATGG GTCATAAACC TCCTATTCCT GACAAGGTGA GCCCAGAAGG GAAAGACTTC 
CTTTGCCACT GTCTGGAAAG TGACCCAAAG ATGAGGTGGA CGGCTAGCCA GCTCCTCGAC 
CATCCCTTTG TCAAGGTTTG CACAGATGAA GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013800899.1
CDS	140..4795
Translation	MRDVAAVALVSAAAAMEEPPPPGRTECKSEDATCKISESEPEELSDEASADTFYGTSPPSTPRQMKRMSTKHQKNNVGRPASRSTLKEKMGLPTQSVHKDNGKTIENVEEHSYKQGKKNRATLRTTERDHKKNVQCSFMLDSVGGSLPKKTIPDVDLNKPYLSLGCSNAKLPVSVPMPIARTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDREQRGQENTSSVWLNRSNELIWLELQAWHAGRSINDQDLYLYAARQAIPDIINEILTFKVNYGSFSCVKNGASLNGTTGEGVCKATHGTSALGYSSYHEHLHRQRVSFEQVKRIMELLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPGPSKDNDPEDEEVDRETEVKESSGMCTEESDGEEQISEENVEELRQAIKNNFTNKPNFDFQDHFSRKLDRLESEDDSASWVIPECSAEAGCSKHCLTSIYRPFVDKALKQMGLRKLILRLHKLMHGSLQRARMALVRNDHQLEFSEFPDPMLCSDYVQLLNSPPSCEKKCSTVSWEELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLREVLDDLQKIDCNIDSFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITPYIRGGEAQAGKLFCDIAGMLLKSTGIFLDSGLQDSCDEFWASADDSTASDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFALSAPVRDFLNALKTKEYVKVQIPGLENLQVFVPNSIAGEKSVILQLLNAAAGKDCSKDSDEVAYEAYLLMTKHSDKDHELDNSWSAWEGQPVKVVPQVETADTLRSMQVDNLLLVVIQSAHLVSQRKAFQQSIEGLISLHHEQTSSQPVIAKALQQLKSDALQLCNKISSAIDRVDRMFTSEFDAEVDESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFSFSSCHSTRSVPCDARNHCSAAVSRPAPIGSDSAQPKPVSSANETRGSSVPENDRLASIAAELQFRSLSRHSSPTEEREDTASKQAPIEAVQKSVQLFEERRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSKLGLQEHVIRLYSKQITTAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNTQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYRVGMGHKPPIPDKVSPEGKDFLCHCLESDPKMRWTASQLLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_013945445.1
Organism	Apteryx australis mantelli
Definition	Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013945445.1

ORF Insert Sequence:

ATGAGAGACG TCGCCGCCGT CGCGCTGGTC TCTGCTGCCG CCGCCATGGA GGAGCCGCCG 
CCGCCGGGGA GGACAGAGTG CAAATCAGAA GATGCTACTT GCAAGATCTC TGAATCTGAA 
CCTGAGGAAT TATCAGATGA AGCAAGTGCT GACACTTTCT ATGGAACTTC TCCTCCTAGT 
ACGCCCCGAC AGATGAAACG TATGTCAACA AAGCATCAGA AAAATAATGT TGGGAGACCT 
GCTAGCCGTT CCACTTTGAA AGAAAAAATG GGTTTACCAA CTCAGTCTGT GCATAAAGAC 
AATGGGAAAA CCATTGAGAA TGTGGAAGAG CACAGCTATA AGCAAGGGAA GAAGAACCGA 
GCCACCCTAA GGACAACAGA ACGAGATCAC AAGAAAAATG TGCAGTGCTC ATTTATGTTA 
GACTCAGTTG GTGGGTCTTT GCCAAAAAAA ACAATTCCAG ATGTTGATCT CAATAAGCCT 
TACCTCAGCC TTGGCTGTAG CAATGCTAAG CTTCCAGTCT CTGTGCCCAT GCCAATAGCC 
AGAACTGCAC GCCAGACTTC TAGGACTGAT TGCCCAGCAG ACCGCTTAAA ATTCTTTGAA 
ACCCTGCGGC TTTTGTTAAA ACTTACCTCA GTCTCGAAGA AAAAGGACAG GGAGCAAAGA 
GGACAGGAAA ACACCTCTTC TGTCTGGCTG AACCGATCCA ATGAACTGAT CTGGCTGGAG 
CTGCAAGCTT GGCATGCTGG CCGGAGCATT AATGACCAAG ACCTATATCT CTATGCGGCC 
CGTCAAGCCA TCCCTGATAT TATCAATGAA ATCCTCACCT TCAAAGTAAA CTATGGAAGC 
TTCTCCTGTG TAAAAAATGG AGCCAGTCTC AATGGTACTA CAGGAGAAGG AGTTTGCAAA 
GCAACACATG GAACTAGTGC TTTGGGTTAC TCAAGTTACC ATGAACACCT CCATCGCCAG 
CGGGTCTCAT TTGAGCAAGT AAAGCGGATA ATGGAGCTGC TAGAGTATAT AGAAGCACTT 
TATCCATCTC TGCAGGCTCT TCAAAAGGAC TATGAAAAGT ATGCTGCAAA GGACTTCCAA 
GACAGAGTGC AGGCACTCTG TCTGTGGTTA AACATTACAA AAGACTTAAA TCAAAAACTA 
AGGATTATGG GAACTGTTTT GGGCATCAAA AATCTTTCTG ACATTGGCTG GCCAGTGTTT 
GAAATTCCTT CTCCTGGACC GTCTAAGGAC AATGATCCAG AGGATGAGGA AGTTGATAGG 
GAAACAGAAG TAAAGGAATC CTCAGGAATG TGCACAGAGG AGAGTGATGG TGAAGAACAA 
ATCTCTGAGG AAAATGTTGA GGAACTTAGA CAAGCCATCA AGAACAATTT TACTAATAAG 
CCAAATTTTG ACTTTCAGGA CCATTTTTCA AGGAAGCTTG ATAGGCTTGA ATCTGAGGAT 
GACTCTGCCT CTTGGGTTAT TCCAGAATGC AGTGCTGAGG CAGGCTGCAG CAAACACTGT 
TTGACCTCTA TTTACAGGCC ATTTGTAGAT AAAGCACTGA AGCAAATGGG GTTGAGGAAG 
CTAATTTTGA GACTGCACAA GCTAATGCAT GGTTCATTGC AAAGGGCCCG TATGGCGTTG 
GTAAGGAACG ATCACCAGCT GGAGTTTTCA GAATTTCCAG ATCCCATGTT GTGTTCAGAT 
TATGTGCAGT TATTAAATTC CCCGCCTTCC TGTGAGAAAA AATGCAGCAC TGTATCATGG 
GAAGAGCTGA AATCTATGGA TTTACCATCT TTTGAACCTG CATTCTTGGT CCTTTGCCGA 
GTCCTGCTCA ATGTTATCCA TGAATGTCTC AAGCTAAGGT TGGAACAAAG ACCTGCTGGG 
GAACCATCTC TTTTGAGTAT TAAGCAGCTA GTAAGAGAGT GTAAAGAGGT CCTGAAGGGC 
GGCCTGTTAA TGAAGCAATA TTATCAGTTC ATGTTACGTG AGGTGTTAGA TGACTTGCAA 
AAGATTGACT GCAACATTGA TTCTTTTGAA GAGGACCTGC ATAAAATGTT AATGGTTTAC 
TTTGATTATA TGAGAAGCTG GATCCAAATG CTACAGCAGT TACCTCAAGC ATCTCATAGT 
TTGAAAAATC TCTTAGAAGA AGAATGGAAT TTCACCAAAG AAATAACTCC TTATATCAGA 
GGAGGTGAAG CTCAAGCTGG AAAACTATTC TGTGATATTG CAGGAATGCT GCTGAAATCT 
ACAGGAATAT TTTTAGACTC TGGACTACAG GACAGTTGTG ATGAGTTTTG GGCCAGTGCT 
GATGATAGTA CTGCATCAGA TGAAATCAGG AGGTCTGTTA TAGAAACCAG CCGAGCACTA 
AAAGAACTCT TTCATGAAGC TAGAGAAAGG GCATCCAAAG CTCTTGGTTT TGCTAAAATG 
CTGAGGAAGG ATCTTGAAAT AGCAGCAGAG TTTGCATTGT CAGCCCCTGT GCGAGATTTT 
CTGAATGCTC TGAAAACAAA AGAGTATGTG AAGGTGCAAA TCCCTGGACT TGAAAACTTA 
CAAGTATTTG TCCCAAATAG TATTGCAGGG GAAAAATCTG TGATTTTACA GCTCCTAAAT 
GCAGCTGCAG GAAAGGATTG CTCGAAGGAT TCGGATGAGG TTGCATATGA GGCCTATTTA 
CTAATGACCA AACACAGTGA CAAAGACCAC GAATTAGACA ACAGTTGGAG CGCGTGGGAG 
GGTCAGCCAG TCAAAGTAGT GCCACAGGTG GAAACTGCTG ATACACTGCG AAGCATGCAG 
GTTGATAACC TCCTCCTTGT AGTCATTCAA TCTGCCCATC TTGTGAGTCA GAGAAAAGCT 
TTCCAACAGT CTATTGAAGG GCTCATATCT TTGCACCACG AACAGACATC CAGCCAACCA 
GTCATTGCCA AAGCTCTGCA ACAGCTAAAG AGTGATGCAT TACAGCTATG CAATAAGATA 
AGCAGTGCCA TTGATAGAGT TGATCGCATG TTCACATCTG AGTTTGATGC TGAAGTTGAT 
GAATCTGAAT CTGCCACTTT GCAACAATAC TACCGGGAAG CCATGATTCA GGGTTATAAT 
TTTGGATTTG AGTACCATAA AGAAGTTGTT CGCCTAATGT CTGGAGAATT CAGGCAGAAA 
ATTGGAGATA AGTATATCAG CTTTGCTAGA AAATGGATGA ATTATGTGCT AACAAAGTGT 
GAGAGTGGCC GAGGCACTCG ACCCAGGTGG GCAACTCAAG GTTTTGATTT TCTTCAAGCC 
ATTGAGCCAG CATTTATTTC AGCTCTCCCA GAAGATGACT TCTTGAGTTT ACAAGCTCTG 
ATGAATGAAT GCATTGGCCA TGTTATTGGA AAACCTCATA GTCCTGTTAC AGCTATTCAT 
CGAAACAGTC CCCGTCCTGT AAAGGTACCT CGATGCCATA GTGATCCACC AAATCCTCAT 
CTTATCATCC CCACTCCTGA AGGTTTCAGT TTTTCTTCTT GCCACAGCAC GCGGAGTGTC 
CCTTGTGATG CACGGAACCA CTGCAGCGCT GCTGTCAGTC GCCCCGCTCC CATTGGTAGC 
GACTCAGCTC AACCCAAACC TGTGAGCAGT GCAAATGAGA CCAGGGGTTC CAGTGTCCCT 
GAAAATGATC GACTGGCTTC AATAGCAGCT GAGTTGCAGT TCAGATCCCT TAGTCGTCAC 
TCCAGTCCCA CTGAAGAGCG AGAAGACACT GCTTCCAAGC AAGCGCCAAT CGAAGCTGTT 
CAGAAGTCTG TTCAGTTGTT TGAAGAAAGG AGATACAGAG AGATGAGGAG GAAGAACATC 
ATTGGCCAAG TTTGTGACAC CCCAAAATCT TATGATAATG TCATGCATGT AGGTTTGAGG 
AAAGTGACTT TCAAGTGGCA GAGAGGAAAC AAAATTGGTG AAGGCCAGTA CGGAAAAGTC 
TACACCTGTA TCAGTGTTGA CACTGGTGAA CTGATGGCTA TGAAAGAAAT AAGATTTCAA 
CCCAATGATC ACAAAACCAT CAAAGAAACA GCAGATGAAC TGAAGATTTT TGAAGGCATC 
AAACACCCTA ATCTTGTTCG TTACTTTGGT GTGGAGCTCC ATAGGGAAGA AATGTACATC 
TTCATGGAGT ACTGTGATGA GGGCACCCTG GAGGAGGTAT CAAAACTGGG CCTTCAGGAA 
CATGTCATTA GGCTCTACTC AAAGCAAATC ACAACTGCTA TCAATGTACT GCATGAACAT 
GGTATTGTTC ATCGAGACAT TAAAGGAGCC AACATCTTTC TTACCTCATC TGGACTGATT 
AAATTAGGAG ACTTTGGCTG CTCAGTGAAA TTAAAGAATA ACACCCAGAC AATGCCTGGA 
GAAGTGAACA GTACTTTGGG GACTGCAGCA TACATGGCTC CAGAAGTCAT TACTAGAGCT 
AAAGGAGAAG GGCATGGGCG TGCAGCAGAC ATCTGGAGCC TGGGCTGTGT TGTAATAGAG 
ATGGTCACTG GCAAGAGGCC GTGGCATGAA TACGAGCACA ACTTCCAGAT CATGTATAGA 
GTGGGGATGG GTCATAAACC TCCTATTCCT GACAAGGTGA GCCCAGAAGG GAAAGACTTC 
CTTTGCCACT GTCTGGAAAG TGACCCAAAG ATGAGGTGGA CGGCTAGCCA GCTCCTCGAC 
CATCCCTTTG TCAAGGTTTG CACAGATGAA GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_013945446.1 ) cDNA ORF clone, Apteryx australis mantelli -> XP_013800900.1 Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X5, mRNA.

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CloneID	OAp16195
Clone ID Related Accession (Same CDS sequence)	XM_013945446.1
Accession Version	XM_013945446.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-08
Organism	Apteryx australis mantelli
Product	mitogen-activated protein kinase kinase kinase 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013993955.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apteryx australis mantelli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGACG TCGCCGCCGT CGCGCTGGTC TCTGCTGCCG CCGCCATGGA GGAGCCGCCG 
CCGCCGGGGA GGACAGAGTG CAAATCAGAA GATGCTACTT GCAAGATCTC TGAATCTGAA 
CCTGAGGAAT TATCAGATGA AGCAAGTGCT GACACTTTCT ATGGAACTTC TCCTCCTAGT 
ACGCCCCGAC AGATGAAACG TATGTCAACA AAGCATCAGA AAAATAATGT TGGGAGACCT 
GCTAGCCGTT CCACTTTGAA AGAAAAAATG GGTTTACCAA CTCAGTCTGT GCATAAAGAC 
AATGGGAAAA CCATTGAGAA TGTGGAAGAG CACAGCTATA AGCAAGGGAA GAAGAACCGA 
GCCACCCTAA GGACAACAGA ACGAGATCAC AAGAAAAATG TGCAGTGCTC ATTTATGTTA 
GACTCAGTTG GTGGGTCTTT GCCAAAAAAA ACAATTCCAG ATGTTGATCT CAATAAGCCT 
TACCTCAGCC TTGGCTGTAG CAATGCTAAG CTTCCAGTCT CTGTGCCCAT GCCAATAGCC 
AGAACTGCAC GCCAGACTTC TAGGACTGAT TGCCCAGCAG ACCGCTTAAA ATTCTTTGAA 
ACCCTGCGGC TTTTGTTAAA ACTTACCTCA GTCTCGAAGA AAAAGGACAG GGAGCAAAGA 
GGACAGGAAA ACACCTCTTC TGTCTGGCTG AACCGATCCA ATGAACTGAT CTGGCTGGAG 
CTGCAAGCTT GGCATGCTGG CCGGAGCATT AATGACCAAG ACCTATATCT CTATGCGGCC 
CGTCAAGCCA TCCCTGATAT TATCAATGAA ATCCTCACCT TCAAAGTAAA CTATGGAAGC 
TTCTCCTGTG TAAAAAATGG AGCCAGTCTC AATGGTACTA CAGGAGAAGG AGTTTGCAAA 
GCAACACATG GAACTAGTGC TTTGGGTTAC TCAAGTTACC ATGAACACCT CCATCGCCAG 
CGGGTCTCAT TTGAGCAAGT AAAGCGGATA ATGGAGCTGC TAGAGTATAT AGAAGCACTT 
TATCCATCTC TGCAGGCTCT TCAAAAGGAC TATGAAAAGT ATGCTGCAAA GGACTTCCAA 
GACAGAGTGC AGGCACTCTG TCTGTGGTTA AACATTACAA AAGACTTAAA TCAAAAACTA 
AGGATTATGG GAACTGTTTT GGGCATCAAA AATCTTTCTG ACATTGGCTG GCCAGTGTTT 
GAAATTCCTT CTCCTGGACC GTCTAAGGAC AATGATCCAG AGGATGAGGA AGTTGATAGG 
GAAACAGAAG TAAAGGAATC CTCAGGAATG TGCACAGAGG AGAGTGATGG TGAAGAACAA 
ATCTCTGAGG AAAATGTTGA GGAACTTAGA CAAGCCATCA AGAACAATTT TACTAATAAG 
CCAAATTTTG ACTTTCAGGA CCATTTTTCA AGGAAGCTTG ATAGGCTTGA ATCTGAGGAT 
GACTCTGCCT CTTGGGTTAT TCCAGAATGC AGTGCTGAGG CAGGCTGCAG CAAACACTGT 
TTGACCTCTA TTTACAGGCC ATTTGTAGAT AAAGCACTGA AGCAAATGGG GTTGAGGAAG 
CTAATTTTGA GACTGCACAA GCTAATGCAT GGTTCATTGC AAAGGGCCCG TATGGCGTTG 
GTAAGGAACG ATCACCAGCT GGAGTTTTCA GAATTTCCAG ATCCCATGTT GTGTTCAGAT 
TATGTGCAGT TATTAAATTC CCCGCCTTCC TGTGAGAAAA AATGCAGCAC TGTATCATGG 
GAAGAGCTGA AATCTATGGA TTTACCATCT TTTGAACCTG CATTCTTGGT CCTTTGCCGA 
GTCCTGCTCA ATGTTATCCA TGAATGTCTC AAGCTAAGGT TGGAACAAAG ACCTGCTGGG 
GAACCATCTC TTTTGAGTAT TAAGCAGCTA GTAAGAGAGT GTAAAGAGGT CCTGAAGGGC 
GGCCTGTTAA TGAAGCAATA TTATCAGTTC ATGTTACGTG AGGTGTTAGA TGACTTGCAA 
AAGATTGACT GCAACATTGA TTCTTTTGAA GAGGACCTGC ATAAAATGTT AATGGTTTAC 
TTTGATTATA TGAGAAGCTG GATCCAAATG CTACAGCAGT TACCTCAAGC ATCTCATAGT 
TTGAAAAATC TCTTAGAAGA AGAATGGAAT TTCACCAAAG AAATAACTCC TTATATCAGA 
GGAGGTGAAG CTCAAGCTGG AAAACTATTC TGTGATATTG CAGGAATGCT GCTGAAATCT 
ACAGGAATAT TTTTAGACTC TGGACTACAG GACAGTTGTG ATGAGTTTTG GGCCAGTGCT 
GATGATAGTA CTGCATCAGA TGAAATCAGG AGGTCTGTTA TAGAAACCAG CCGAGCACTA 
AAAGAACTCT TTCATGAAGC TAGAGAAAGG GCATCCAAAG CTCTTGGTTT TGCTAAAATG 
CTGAGGAAGG ATCTTGAAAT AGCAGCAGAG TTTGCATTGT CAGCCCCTGT GCGAGATTTT 
CTGAATGCTC TGAAAACAAA AGAGTATGTG AAGGTGCAAA TCCCTGGACT TGAAAACTTA 
CAAGTATTTG TCCCAAATAG TATTGCAGGG GAAAAATCTG TGATTTTACA GCTCCTAAAT 
GCAGCTGCAG GAAAGGATTG CTCGAAGGAT TCGGATGAGG TTGCATATGA GGCCTATTTA 
CTAATGACCA AACACAGTGA CAAAGACCAC GAATTAGACA ACAGTTGGAG CGCGTGGGAG 
GGTCAGCCAG TCAAAGTAGT GCCACAGGTG GAAACTGCTG ATACACTGCG AAGCATGCAG 
GTTGATAACC TCCTCCTTGT AGTCATTCAA TCTGCCCATC TTGTGAGTCA GAGAAAAGCT 
TTCCAACAGT CTATTGAAGG GCTCATATCT TTGCACCACG AACAGACATC CAGCCAACCA 
GTCATTGCCA AAGCTCTGCA ACAGCTAAAG AGTGATGCAT TACAGCTATG CAATAAGATA 
AGCAGTGCCA TTGATAGAGT TGATCGCATG TTCACATCTG AGTTTGATGC TGAAGTTGAT 
GAATCTGAAT CTGCCACTTT GCAACAATAC TACCGGGAAG CCATGATTCA GGGTTATAAT 
TTTGGATTTG AGTACCATAA AGAAGTTGTT CGCCTAATGT CTGGAGAATT CAGGCAGAAA 
ATTGGAGATA AGTATATCAG CTTTGCTAGA AAATGGATGA ATTATGTGCT AACAAAGTGT 
GAGAGTGGCC GAGGCACTCG ACCCAGGTGG GCAACTCAAG GTTTTGATTT TCTTCAAGCC 
ATTGAGCCAG CATTTATTTC AGCTCTCCCA GAAGATGACT TCTTGAGTTT ACAAGCTCTG 
ATGAATGAAT GCATTGGCCA TGTTATTGGA AAACCTCATA GTCCTGTTAC AGGTTTGTAC 
CTTGCTATTC ATCGAAACAG TCCCCGTCCT GTAAAGGTAC CTCGATGCCA TAGTGATCCA 
CCAAATCCTC ATCTTATCAT CCCCACTCCT GAAGGTTTCA GGGGTTCCAG TGTCCCTGAA 
AATGATCGAC TGGCTTCAAT AGCAGCTGAG TTGCAGTTCA GATCCCTTAG TCGTCACTCC 
AGTCCCACTG AAGAGCGAGA AGAACCATCT TATCCAAAAG GAGATTCAGG TGGATCATCC 
CGGCGGAGTT GGGAGCTCAG AACTTTAATC AACCAGACCA AAGACACTGC TTCCAAGCAA 
GCGCCAATCG AAGCTGTTCA GAAGTCTGTT CAGTTGTTTG AAGAAAGGAG ATACAGAGAG 
ATGAGGAGGA AGAACATCAT TGGCCAAGTT TGTGACACCC CAAAATCTTA TGATAATGTC 
ATGCATGTAG GTTTGAGGAA AGTGACTTTC AAGTGGCAGA GAGGAAACAA AATTGGTGAA 
GGCCAGTACG GAAAAGTCTA CACCTGTATC AGTGTTGACA CTGGTGAACT GATGGCTATG 
AAAGAAATAA GATTTCAACC CAATGATCAC AAAACCATCA AAGAAACAGC AGATGAACTG 
AAGATTTTTG AAGGCATCAA ACACCCTAAT CTTGTTCGTT ACTTTGGTGT GGAGCTCCAT 
AGGGAAGAAA TGTACATCTT CATGGAGTAC TGTGATGAGG GCACCCTGGA GGAGGTATCA 
AAACTGGGCC TTCAGGAACA TGTCATTAGG CTCTACTCAA AGCAAATCAC AACTGCTATC 
AATGTACTGC ATGAACATGG TATTGTTCAT CGAGACATTA AAGGAGCCAA CATCTTTCTT 
ACCTCATCTG GACTGATTAA ATTAGGAGAC TTTGGCTGCT CAGTGAAATT AAAGAATAAC 
ACCCAGACAA TGCCTGGAGA AGTGAACAGT ACTTTGGGGA CTGCAGCATA CATGGCTCCA 
GAAGTCATTA CTAGAGCTAA AGGAGAAGGG CATGGGCGTG CAGCAGACAT CTGGAGCCTG 
GGCTGTGTTG TAATAGAGAT GGTCACTGGC AAGAGGCCGT GGCATGAATA CGAGCACAAC 
TTCCAGATCA TGTATAGAGT GGGGATGGGT CATAAACCTC CTATTCCTGA CAAGGTGAGC 
CCAGAAGGGA AAGACTTCCT TTGCCACTGT CTGGAAAGTG ACCCAAAGAT GAGGTGGACG 
GCTAGCCAGC TCCTCGACCA TCCCTTTGTC AAGGTTTGCA CAGATGAAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013800900.1
CDS	140..4753
Translation	MRDVAAVALVSAAAAMEEPPPPGRTECKSEDATCKISESEPEELSDEASADTFYGTSPPSTPRQMKRMSTKHQKNNVGRPASRSTLKEKMGLPTQSVHKDNGKTIENVEEHSYKQGKKNRATLRTTERDHKKNVQCSFMLDSVGGSLPKKTIPDVDLNKPYLSLGCSNAKLPVSVPMPIARTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDREQRGQENTSSVWLNRSNELIWLELQAWHAGRSINDQDLYLYAARQAIPDIINEILTFKVNYGSFSCVKNGASLNGTTGEGVCKATHGTSALGYSSYHEHLHRQRVSFEQVKRIMELLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPGPSKDNDPEDEEVDRETEVKESSGMCTEESDGEEQISEENVEELRQAIKNNFTNKPNFDFQDHFSRKLDRLESEDDSASWVIPECSAEAGCSKHCLTSIYRPFVDKALKQMGLRKLILRLHKLMHGSLQRARMALVRNDHQLEFSEFPDPMLCSDYVQLLNSPPSCEKKCSTVSWEELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLREVLDDLQKIDCNIDSFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITPYIRGGEAQAGKLFCDIAGMLLKSTGIFLDSGLQDSCDEFWASADDSTASDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFALSAPVRDFLNALKTKEYVKVQIPGLENLQVFVPNSIAGEKSVILQLLNAAAGKDCSKDSDEVAYEAYLLMTKHSDKDHELDNSWSAWEGQPVKVVPQVETADTLRSMQVDNLLLVVIQSAHLVSQRKAFQQSIEGLISLHHEQTSSQPVIAKALQQLKSDALQLCNKISSAIDRVDRMFTSEFDAEVDESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYLAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFRGSSVPENDRLASIAAELQFRSLSRHSSPTEEREEPSYPKGDSGGSSRRSWELRTLINQTKDTASKQAPIEAVQKSVQLFEERRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSKLGLQEHVIRLYSKQITTAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNTQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYRVGMGHKPPIPDKVSPEGKDFLCHCLESDPKMRWTASQLLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_013945446.1
Organism	Apteryx australis mantelli
Definition	Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013945446.1

ORF Insert Sequence:

ATGAGAGACG TCGCCGCCGT CGCGCTGGTC TCTGCTGCCG CCGCCATGGA GGAGCCGCCG 
CCGCCGGGGA GGACAGAGTG CAAATCAGAA GATGCTACTT GCAAGATCTC TGAATCTGAA 
CCTGAGGAAT TATCAGATGA AGCAAGTGCT GACACTTTCT ATGGAACTTC TCCTCCTAGT 
ACGCCCCGAC AGATGAAACG TATGTCAACA AAGCATCAGA AAAATAATGT TGGGAGACCT 
GCTAGCCGTT CCACTTTGAA AGAAAAAATG GGTTTACCAA CTCAGTCTGT GCATAAAGAC 
AATGGGAAAA CCATTGAGAA TGTGGAAGAG CACAGCTATA AGCAAGGGAA GAAGAACCGA 
GCCACCCTAA GGACAACAGA ACGAGATCAC AAGAAAAATG TGCAGTGCTC ATTTATGTTA 
GACTCAGTTG GTGGGTCTTT GCCAAAAAAA ACAATTCCAG ATGTTGATCT CAATAAGCCT 
TACCTCAGCC TTGGCTGTAG CAATGCTAAG CTTCCAGTCT CTGTGCCCAT GCCAATAGCC 
AGAACTGCAC GCCAGACTTC TAGGACTGAT TGCCCAGCAG ACCGCTTAAA ATTCTTTGAA 
ACCCTGCGGC TTTTGTTAAA ACTTACCTCA GTCTCGAAGA AAAAGGACAG GGAGCAAAGA 
GGACAGGAAA ACACCTCTTC TGTCTGGCTG AACCGATCCA ATGAACTGAT CTGGCTGGAG 
CTGCAAGCTT GGCATGCTGG CCGGAGCATT AATGACCAAG ACCTATATCT CTATGCGGCC 
CGTCAAGCCA TCCCTGATAT TATCAATGAA ATCCTCACCT TCAAAGTAAA CTATGGAAGC 
TTCTCCTGTG TAAAAAATGG AGCCAGTCTC AATGGTACTA CAGGAGAAGG AGTTTGCAAA 
GCAACACATG GAACTAGTGC TTTGGGTTAC TCAAGTTACC ATGAACACCT CCATCGCCAG 
CGGGTCTCAT TTGAGCAAGT AAAGCGGATA ATGGAGCTGC TAGAGTATAT AGAAGCACTT 
TATCCATCTC TGCAGGCTCT TCAAAAGGAC TATGAAAAGT ATGCTGCAAA GGACTTCCAA 
GACAGAGTGC AGGCACTCTG TCTGTGGTTA AACATTACAA AAGACTTAAA TCAAAAACTA 
AGGATTATGG GAACTGTTTT GGGCATCAAA AATCTTTCTG ACATTGGCTG GCCAGTGTTT 
GAAATTCCTT CTCCTGGACC GTCTAAGGAC AATGATCCAG AGGATGAGGA AGTTGATAGG 
GAAACAGAAG TAAAGGAATC CTCAGGAATG TGCACAGAGG AGAGTGATGG TGAAGAACAA 
ATCTCTGAGG AAAATGTTGA GGAACTTAGA CAAGCCATCA AGAACAATTT TACTAATAAG 
CCAAATTTTG ACTTTCAGGA CCATTTTTCA AGGAAGCTTG ATAGGCTTGA ATCTGAGGAT 
GACTCTGCCT CTTGGGTTAT TCCAGAATGC AGTGCTGAGG CAGGCTGCAG CAAACACTGT 
TTGACCTCTA TTTACAGGCC ATTTGTAGAT AAAGCACTGA AGCAAATGGG GTTGAGGAAG 
CTAATTTTGA GACTGCACAA GCTAATGCAT GGTTCATTGC AAAGGGCCCG TATGGCGTTG 
GTAAGGAACG ATCACCAGCT GGAGTTTTCA GAATTTCCAG ATCCCATGTT GTGTTCAGAT 
TATGTGCAGT TATTAAATTC CCCGCCTTCC TGTGAGAAAA AATGCAGCAC TGTATCATGG 
GAAGAGCTGA AATCTATGGA TTTACCATCT TTTGAACCTG CATTCTTGGT CCTTTGCCGA 
GTCCTGCTCA ATGTTATCCA TGAATGTCTC AAGCTAAGGT TGGAACAAAG ACCTGCTGGG 
GAACCATCTC TTTTGAGTAT TAAGCAGCTA GTAAGAGAGT GTAAAGAGGT CCTGAAGGGC 
GGCCTGTTAA TGAAGCAATA TTATCAGTTC ATGTTACGTG AGGTGTTAGA TGACTTGCAA 
AAGATTGACT GCAACATTGA TTCTTTTGAA GAGGACCTGC ATAAAATGTT AATGGTTTAC 
TTTGATTATA TGAGAAGCTG GATCCAAATG CTACAGCAGT TACCTCAAGC ATCTCATAGT 
TTGAAAAATC TCTTAGAAGA AGAATGGAAT TTCACCAAAG AAATAACTCC TTATATCAGA 
GGAGGTGAAG CTCAAGCTGG AAAACTATTC TGTGATATTG CAGGAATGCT GCTGAAATCT 
ACAGGAATAT TTTTAGACTC TGGACTACAG GACAGTTGTG ATGAGTTTTG GGCCAGTGCT 
GATGATAGTA CTGCATCAGA TGAAATCAGG AGGTCTGTTA TAGAAACCAG CCGAGCACTA 
AAAGAACTCT TTCATGAAGC TAGAGAAAGG GCATCCAAAG CTCTTGGTTT TGCTAAAATG 
CTGAGGAAGG ATCTTGAAAT AGCAGCAGAG TTTGCATTGT CAGCCCCTGT GCGAGATTTT 
CTGAATGCTC TGAAAACAAA AGAGTATGTG AAGGTGCAAA TCCCTGGACT TGAAAACTTA 
CAAGTATTTG TCCCAAATAG TATTGCAGGG GAAAAATCTG TGATTTTACA GCTCCTAAAT 
GCAGCTGCAG GAAAGGATTG CTCGAAGGAT TCGGATGAGG TTGCATATGA GGCCTATTTA 
CTAATGACCA AACACAGTGA CAAAGACCAC GAATTAGACA ACAGTTGGAG CGCGTGGGAG 
GGTCAGCCAG TCAAAGTAGT GCCACAGGTG GAAACTGCTG ATACACTGCG AAGCATGCAG 
GTTGATAACC TCCTCCTTGT AGTCATTCAA TCTGCCCATC TTGTGAGTCA GAGAAAAGCT 
TTCCAACAGT CTATTGAAGG GCTCATATCT TTGCACCACG AACAGACATC CAGCCAACCA 
GTCATTGCCA AAGCTCTGCA ACAGCTAAAG AGTGATGCAT TACAGCTATG CAATAAGATA 
AGCAGTGCCA TTGATAGAGT TGATCGCATG TTCACATCTG AGTTTGATGC TGAAGTTGAT 
GAATCTGAAT CTGCCACTTT GCAACAATAC TACCGGGAAG CCATGATTCA GGGTTATAAT 
TTTGGATTTG AGTACCATAA AGAAGTTGTT CGCCTAATGT CTGGAGAATT CAGGCAGAAA 
ATTGGAGATA AGTATATCAG CTTTGCTAGA AAATGGATGA ATTATGTGCT AACAAAGTGT 
GAGAGTGGCC GAGGCACTCG ACCCAGGTGG GCAACTCAAG GTTTTGATTT TCTTCAAGCC 
ATTGAGCCAG CATTTATTTC AGCTCTCCCA GAAGATGACT TCTTGAGTTT ACAAGCTCTG 
ATGAATGAAT GCATTGGCCA TGTTATTGGA AAACCTCATA GTCCTGTTAC AGGTTTGTAC 
CTTGCTATTC ATCGAAACAG TCCCCGTCCT GTAAAGGTAC CTCGATGCCA TAGTGATCCA 
CCAAATCCTC ATCTTATCAT CCCCACTCCT GAAGGTTTCA GGGGTTCCAG TGTCCCTGAA 
AATGATCGAC TGGCTTCAAT AGCAGCTGAG TTGCAGTTCA GATCCCTTAG TCGTCACTCC 
AGTCCCACTG AAGAGCGAGA AGAACCATCT TATCCAAAAG GAGATTCAGG TGGATCATCC 
CGGCGGAGTT GGGAGCTCAG AACTTTAATC AACCAGACCA AAGACACTGC TTCCAAGCAA 
GCGCCAATCG AAGCTGTTCA GAAGTCTGTT CAGTTGTTTG AAGAAAGGAG ATACAGAGAG 
ATGAGGAGGA AGAACATCAT TGGCCAAGTT TGTGACACCC CAAAATCTTA TGATAATGTC 
ATGCATGTAG GTTTGAGGAA AGTGACTTTC AAGTGGCAGA GAGGAAACAA AATTGGTGAA 
GGCCAGTACG GAAAAGTCTA CACCTGTATC AGTGTTGACA CTGGTGAACT GATGGCTATG 
AAAGAAATAA GATTTCAACC CAATGATCAC AAAACCATCA AAGAAACAGC AGATGAACTG 
AAGATTTTTG AAGGCATCAA ACACCCTAAT CTTGTTCGTT ACTTTGGTGT GGAGCTCCAT 
AGGGAAGAAA TGTACATCTT CATGGAGTAC TGTGATGAGG GCACCCTGGA GGAGGTATCA 
AAACTGGGCC TTCAGGAACA TGTCATTAGG CTCTACTCAA AGCAAATCAC AACTGCTATC 
AATGTACTGC ATGAACATGG TATTGTTCAT CGAGACATTA AAGGAGCCAA CATCTTTCTT 
ACCTCATCTG GACTGATTAA ATTAGGAGAC TTTGGCTGCT CAGTGAAATT AAAGAATAAC 
ACCCAGACAA TGCCTGGAGA AGTGAACAGT ACTTTGGGGA CTGCAGCATA CATGGCTCCA 
GAAGTCATTA CTAGAGCTAA AGGAGAAGGG CATGGGCGTG CAGCAGACAT CTGGAGCCTG 
GGCTGTGTTG TAATAGAGAT GGTCACTGGC AAGAGGCCGT GGCATGAATA CGAGCACAAC 
TTCCAGATCA TGTATAGAGT GGGGATGGGT CATAAACCTC CTATTCCTGA CAAGGTGAGC 
CCAGAAGGGA AAGACTTCCT TTGCCACTGT CTGGAAAGTG ACCCAAAGAT GAGGTGGACG 
GCTAGCCAGC TCCTCGACCA TCCCTTTGTC AAGGTTTGCA CAGATGAAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_013945447.1 ) cDNA ORF clone, Apteryx australis mantelli -> XP_013800901.1 Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X6, mRNA.

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CloneID	OAp16196
Clone ID Related Accession (Same CDS sequence)	XM_013945447.1
Accession Version	XM_013945447.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-08
Organism	Apteryx australis mantelli
Product	mitogen-activated protein kinase kinase kinase 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013993955.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apteryx australis mantelli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGACG TCGCCGCCGT CGCGCTGGTC TCTGCTGCCG CCGCCATGGA GGAGCCGCCG 
CCGCCGGGGA GGACAGAGTG CAAATCAGAA GATGCTACTT GCAAGATCTC TGAATCTGAA 
CCTGAGGAAT TATCAGATGA AGCAAGTGCT GACACTTTCT ATGGAACTTC TCCTCCTAGT 
ACGCCCCGAC AGATGAAACG TATGTCAACA AAGCATCAGA AAAATAATGT TGGGAGACCT 
GCTAGCCGTT CCACTTTGAA AGAAAAAATG GGTTTACCAA CTCAGTCTGT GCATAAAGAC 
AATGGGAAAA CCATTGAGAA TGTGGAAGAG CACAGCTATA AGCAAGGGAA GAAGAACCGA 
GCCACCCTAA GGACAACAGA ACGAGATCAC AAGAAAAATG TGCAGTGCTC ATTTATGTTA 
GACTCAGTTG GTGGGTCTTT GCCAAAAAAA ACAATTCCAG ATGTTGATCT CAATAAGCCT 
TACCTCAGCC TTGGCTGTAG CAATGCTAAG CTTCCAGTCT CTGTGCCCAT GCCAATAGCC 
AGAACTGCAC GCCAGACTTC TAGGACTGAT TGCCCAGCAG ACCGCTTAAA ATTCTTTGAA 
ACCCTGCGGC TTTTGTTAAA ACTTACCTCA GTCTCGAAGA AAAAGGACAG GGAGCAAAGA 
GGACAGGAAA ACACCTCTTC TGTCTGGCTG AACCGATCCA ATGAACTGAT CTGGCTGGAG 
CTGCAAGCTT GGCATGCTGG CCGGAGCATT AATGACCAAG ACCTATATCT CTATGCGGCC 
CGTCAAGCCA TCCCTGATAT TATCAATGAA ATCCTCACCT TCAAAGTAAA CTATGGAAGC 
TTCTCCTGTG TAAAAAATGG AGCCAGTCTC AATGGTACTA CAGGAGAAGG AGTTTGCAAA 
GCAACACATG GAACTAGTGC TTTGGGTTAC TCAAGTTACC ATGAACACCT CCATCGCCAG 
CGGGTCTCAT TTGAGCAAGT AAAGCGGATA ATGGAGCTGC TAGAGTATAT AGAAGCACTT 
TATCCATCTC TGCAGGCTCT TCAAAAGGAC TATGAAAAGT ATGCTGCAAA GGACTTCCAA 
GACAGAGTGC AGGCACTCTG TCTGTGGTTA AACATTACAA AAGACTTAAA TCAAAAACTA 
AGGATTATGG GAACTGTTTT GGGCATCAAA AATCTTTCTG ACATTGGCTG GCCAGTGTTT 
GAAATTCCTT CTCCTGGACC GTCTAAGGAC AATGATCCAG AGGATGAGGA AGTTGATAGG 
GAAACAGAAG TAAAGGAATC CTCAGGAATG TGCACAGAGG AGAGTGATGG TGAAGAACAA 
ATCTCTGAGG AAAATGTTGA GGAACTTAGA CAAGCCATCA AGAACAATTT TACTAATAAG 
CCAAATTTTG ACTTTCAGGA CCATTTTTCA AGGAAGCTTG ATAGGCTTGA ATCTGAGGAT 
GACTCTGCCT CTTGGGTTAT TCCAGAATGC AGTGCTGAGG CAGGCTGCAG CAAACACTGT 
TTGACCTCTA TTTACAGGCC ATTTGTAGAT AAAGCACTGA AGCAAATGGG GTTGAGGAAG 
CTAATTTTGA GACTGCACAA GCTAATGCAT GGTTCATTGC AAAGGGCCCG TATGGCGTTG 
GTAAGGAACG ATCACCAGCT GGAGTTTTCA GAATTTCCAG ATCCCATGTT GTGTTCAGAT 
TATGTGCAGT TATTAAATTC CCCGCCTTCC TGTGAGAAAA AATGCAGCAC TGTATCATGG 
GAAGAGCTGA AATCTATGGA TTTACCATCT TTTGAACCTG CATTCTTGGT CCTTTGCCGA 
GTCCTGCTCA ATGTTATCCA TGAATGTCTC AAGCTAAGGT TGGAACAAAG ACCTGCTGGG 
GAACCATCTC TTTTGAGTAT TAAGCAGCTA GTAAGAGAGT GTAAAGAGGT CCTGAAGGGC 
GGCCTGTTAA TGAAGCAATA TTATCAGTTC ATGTTACGTG AGGTGTTAGA TGACTTGCAA 
AAGATTGACT GCAACATTGA TTCTTTTGAA GAGGACCTGC ATAAAATGTT AATGGTTTAC 
TTTGATTATA TGAGAAGCTG GATCCAAATG CTACAGCAGT TACCTCAAGC ATCTCATAGT 
TTGAAAAATC TCTTAGAAGA AGAATGGAAT TTCACCAAAG AAATAACTCC TTATATCAGA 
GGAGGTGAAG CTCAAGCTGG AAAACTATTC TGTGATATTG CAGGAATGCT GCTGAAATCT 
ACAGGAATAT TTTTAGACTC TGGACTACAG GACAGTTGTG ATGAGTTTTG GGCCAGTGCT 
GATGATAGTA CTGCATCAGA TGAAATCAGG AGGTCTGTTA TAGAAACCAG CCGAGCACTA 
AAAGAACTCT TTCATGAAGC TAGAGAAAGG GCATCCAAAG CTCTTGGTTT TGCTAAAATG 
CTGAGGAAGG ATCTTGAAAT AGCAGCAGAG TTTGCATTGT CAGCCCCTGT GCGAGATTTT 
CTGAATGCTC TGAAAACAAA AGAGTATGTG AAGGTGCAAA TCCCTGGACT TGAAAACTTA 
CAAGTATTTG TCCCAAATAG TATTGCAGGG GAAAAATCTG TGATTTTACA GCTCCTAAAT 
GCAGCTGCAG GAAAGGATTG CTCGAAGGAT TCGGATGAGG TTGCATATGA GGCCTATTTA 
CTAATGACCA AACACAGTGA CAAAGACCAC GAATTAGACA ACAGTTGGAG CGCGTGGGAG 
GGTCAGCCAG TCAAAGTAGT GCCACAGGTG GAAACTGCTG ATACACTGCG AAGCATGCAG 
GTTGATAACC TCCTCCTTGT AGTCATTCAA TCTGCCCATC TTGTGAGTCA GAGAAAAGCT 
TTCCAACAGT CTATTGAAGG GCTCATATCT TTGCACCACG AACAGACATC CAGCCAACCA 
GTCATTGCCA AAGCTCTGCA ACAGCTAAAG AGTGATGCAT TACAGCTATG CAATAAGATA 
AGCAGTGCCA TTGATAGAGT TGATCGCATG TTCACATCTG AGTTTGATGC TGAAGTTGAT 
GAATCTGAAT CTGCCACTTT GCAACAATAC TACCGGGAAG CCATGATTCA GGGTTATAAT 
TTTGGATTTG AGTACCATAA AGAAGTTGTT CGCCTAATGT CTGGAGAATT CAGGCAGAAA 
ATTGGAGATA AGTATATCAG CTTTGCTAGA AAATGGATGA ATTATGTGCT AACAAAGTGT 
GAGAGTGGCC GAGGCACTCG ACCCAGGTGG GCAACTCAAG GTTTTGATTT TCTTCAAGCC 
ATTGAGCCAG CATTTATTTC AGCTCTCCCA GAAGATGACT TCTTGAGTTT ACAAGCTCTG 
ATGAATGAAT GCATTGGCCA TGTTATTGGA AAACCTCATA GTCCTGTTAC AGCTATTCAT 
CGAAACAGTC CCCGTCCTGT AAAGGTACCT CGATGCCATA GTGATCCACC AAATCCTCAT 
CTTATCATCC CCACTCCTGA AGGTTTCAGG GGTTCCAGTG TCCCTGAAAA TGATCGACTG 
GCTTCAATAG CAGCTGAGTT GCAGTTCAGA TCCCTTAGTC GTCACTCCAG TCCCACTGAA 
GAGCGAGAAG AACCATCTTA TCCAAAAGGA GATTCAGGTG GATCATCCCG GCGGAGTTGG 
GAGCTCAGAA CTTTAATCAA CCAGACCAAA GACACTGCTT CCAAGCAAGC GCCAATCGAA 
GCTGTTCAGA AGTCTGTTCA GTTGTTTGAA GAAAGGAGAT ACAGAGAGAT GAGGAGGAAG 
AACATCATTG GCCAAGTTTG TGACACCCCA AAATCTTATG ATAATGTCAT GCATGTAGGT 
TTGAGGAAAG TGACTTTCAA GTGGCAGAGA GGAAACAAAA TTGGTGAAGG CCAGTACGGA 
AAAGTCTACA CCTGTATCAG TGTTGACACT GGTGAACTGA TGGCTATGAA AGAAATAAGA 
TTTCAACCCA ATGATCACAA AACCATCAAA GAAACAGCAG ATGAACTGAA GATTTTTGAA 
GGCATCAAAC ACCCTAATCT TGTTCGTTAC TTTGGTGTGG AGCTCCATAG GGAAGAAATG 
TACATCTTCA TGGAGTACTG TGATGAGGGC ACCCTGGAGG AGGTATCAAA ACTGGGCCTT 
CAGGAACATG TCATTAGGCT CTACTCAAAG CAAATCACAA CTGCTATCAA TGTACTGCAT 
GAACATGGTA TTGTTCATCG AGACATTAAA GGAGCCAACA TCTTTCTTAC CTCATCTGGA 
CTGATTAAAT TAGGAGACTT TGGCTGCTCA GTGAAATTAA AGAATAACAC CCAGACAATG 
CCTGGAGAAG TGAACAGTAC TTTGGGGACT GCAGCATACA TGGCTCCAGA AGTCATTACT 
AGAGCTAAAG GAGAAGGGCA TGGGCGTGCA GCAGACATCT GGAGCCTGGG CTGTGTTGTA 
ATAGAGATGG TCACTGGCAA GAGGCCGTGG CATGAATACG AGCACAACTT CCAGATCATG 
TATAGAGTGG GGATGGGTCA TAAACCTCCT ATTCCTGACA AGGTGAGCCC AGAAGGGAAA 
GACTTCCTTT GCCACTGTCT GGAAAGTGAC CCAAAGATGA GGTGGACGGC TAGCCAGCTC 
CTCGACCATC CCTTTGTCAA GGTTTGCACA GATGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013800901.1
CDS	140..4741
Translation	MRDVAAVALVSAAAAMEEPPPPGRTECKSEDATCKISESEPEELSDEASADTFYGTSPPSTPRQMKRMSTKHQKNNVGRPASRSTLKEKMGLPTQSVHKDNGKTIENVEEHSYKQGKKNRATLRTTERDHKKNVQCSFMLDSVGGSLPKKTIPDVDLNKPYLSLGCSNAKLPVSVPMPIARTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDREQRGQENTSSVWLNRSNELIWLELQAWHAGRSINDQDLYLYAARQAIPDIINEILTFKVNYGSFSCVKNGASLNGTTGEGVCKATHGTSALGYSSYHEHLHRQRVSFEQVKRIMELLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPGPSKDNDPEDEEVDRETEVKESSGMCTEESDGEEQISEENVEELRQAIKNNFTNKPNFDFQDHFSRKLDRLESEDDSASWVIPECSAEAGCSKHCLTSIYRPFVDKALKQMGLRKLILRLHKLMHGSLQRARMALVRNDHQLEFSEFPDPMLCSDYVQLLNSPPSCEKKCSTVSWEELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLREVLDDLQKIDCNIDSFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITPYIRGGEAQAGKLFCDIAGMLLKSTGIFLDSGLQDSCDEFWASADDSTASDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFALSAPVRDFLNALKTKEYVKVQIPGLENLQVFVPNSIAGEKSVILQLLNAAAGKDCSKDSDEVAYEAYLLMTKHSDKDHELDNSWSAWEGQPVKVVPQVETADTLRSMQVDNLLLVVIQSAHLVSQRKAFQQSIEGLISLHHEQTSSQPVIAKALQQLKSDALQLCNKISSAIDRVDRMFTSEFDAEVDESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFRGSSVPENDRLASIAAELQFRSLSRHSSPTEEREEPSYPKGDSGGSSRRSWELRTLINQTKDTASKQAPIEAVQKSVQLFEERRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSKLGLQEHVIRLYSKQITTAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNTQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYRVGMGHKPPIPDKVSPEGKDFLCHCLESDPKMRWTASQLLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_013945447.1
Organism	Apteryx australis mantelli
Definition	Apteryx australis mantelli mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013945447.1

ORF Insert Sequence:

ATGAGAGACG TCGCCGCCGT CGCGCTGGTC TCTGCTGCCG CCGCCATGGA GGAGCCGCCG 
CCGCCGGGGA GGACAGAGTG CAAATCAGAA GATGCTACTT GCAAGATCTC TGAATCTGAA 
CCTGAGGAAT TATCAGATGA AGCAAGTGCT GACACTTTCT ATGGAACTTC TCCTCCTAGT 
ACGCCCCGAC AGATGAAACG TATGTCAACA AAGCATCAGA AAAATAATGT TGGGAGACCT 
GCTAGCCGTT CCACTTTGAA AGAAAAAATG GGTTTACCAA CTCAGTCTGT GCATAAAGAC 
AATGGGAAAA CCATTGAGAA TGTGGAAGAG CACAGCTATA AGCAAGGGAA GAAGAACCGA 
GCCACCCTAA GGACAACAGA ACGAGATCAC AAGAAAAATG TGCAGTGCTC ATTTATGTTA 
GACTCAGTTG GTGGGTCTTT GCCAAAAAAA ACAATTCCAG ATGTTGATCT CAATAAGCCT 
TACCTCAGCC TTGGCTGTAG CAATGCTAAG CTTCCAGTCT CTGTGCCCAT GCCAATAGCC 
AGAACTGCAC GCCAGACTTC TAGGACTGAT TGCCCAGCAG ACCGCTTAAA ATTCTTTGAA 
ACCCTGCGGC TTTTGTTAAA ACTTACCTCA GTCTCGAAGA AAAAGGACAG GGAGCAAAGA 
GGACAGGAAA ACACCTCTTC TGTCTGGCTG AACCGATCCA ATGAACTGAT CTGGCTGGAG 
CTGCAAGCTT GGCATGCTGG CCGGAGCATT AATGACCAAG ACCTATATCT CTATGCGGCC 
CGTCAAGCCA TCCCTGATAT TATCAATGAA ATCCTCACCT TCAAAGTAAA CTATGGAAGC 
TTCTCCTGTG TAAAAAATGG AGCCAGTCTC AATGGTACTA CAGGAGAAGG AGTTTGCAAA 
GCAACACATG GAACTAGTGC TTTGGGTTAC TCAAGTTACC ATGAACACCT CCATCGCCAG 
CGGGTCTCAT TTGAGCAAGT AAAGCGGATA ATGGAGCTGC TAGAGTATAT AGAAGCACTT 
TATCCATCTC TGCAGGCTCT TCAAAAGGAC TATGAAAAGT ATGCTGCAAA GGACTTCCAA 
GACAGAGTGC AGGCACTCTG TCTGTGGTTA AACATTACAA AAGACTTAAA TCAAAAACTA 
AGGATTATGG GAACTGTTTT GGGCATCAAA AATCTTTCTG ACATTGGCTG GCCAGTGTTT 
GAAATTCCTT CTCCTGGACC GTCTAAGGAC AATGATCCAG AGGATGAGGA AGTTGATAGG 
GAAACAGAAG TAAAGGAATC CTCAGGAATG TGCACAGAGG AGAGTGATGG TGAAGAACAA 
ATCTCTGAGG AAAATGTTGA GGAACTTAGA CAAGCCATCA AGAACAATTT TACTAATAAG 
CCAAATTTTG ACTTTCAGGA CCATTTTTCA AGGAAGCTTG ATAGGCTTGA ATCTGAGGAT 
GACTCTGCCT CTTGGGTTAT TCCAGAATGC AGTGCTGAGG CAGGCTGCAG CAAACACTGT 
TTGACCTCTA TTTACAGGCC ATTTGTAGAT AAAGCACTGA AGCAAATGGG GTTGAGGAAG 
CTAATTTTGA GACTGCACAA GCTAATGCAT GGTTCATTGC AAAGGGCCCG TATGGCGTTG 
GTAAGGAACG ATCACCAGCT GGAGTTTTCA GAATTTCCAG ATCCCATGTT GTGTTCAGAT 
TATGTGCAGT TATTAAATTC CCCGCCTTCC TGTGAGAAAA AATGCAGCAC TGTATCATGG 
GAAGAGCTGA AATCTATGGA TTTACCATCT TTTGAACCTG CATTCTTGGT CCTTTGCCGA 
GTCCTGCTCA ATGTTATCCA TGAATGTCTC AAGCTAAGGT TGGAACAAAG ACCTGCTGGG 
GAACCATCTC TTTTGAGTAT TAAGCAGCTA GTAAGAGAGT GTAAAGAGGT CCTGAAGGGC 
GGCCTGTTAA TGAAGCAATA TTATCAGTTC ATGTTACGTG AGGTGTTAGA TGACTTGCAA 
AAGATTGACT GCAACATTGA TTCTTTTGAA GAGGACCTGC ATAAAATGTT AATGGTTTAC 
TTTGATTATA TGAGAAGCTG GATCCAAATG CTACAGCAGT TACCTCAAGC ATCTCATAGT 
TTGAAAAATC TCTTAGAAGA AGAATGGAAT TTCACCAAAG AAATAACTCC TTATATCAGA 
GGAGGTGAAG CTCAAGCTGG AAAACTATTC TGTGATATTG CAGGAATGCT GCTGAAATCT 
ACAGGAATAT TTTTAGACTC TGGACTACAG GACAGTTGTG ATGAGTTTTG GGCCAGTGCT 
GATGATAGTA CTGCATCAGA TGAAATCAGG AGGTCTGTTA TAGAAACCAG CCGAGCACTA 
AAAGAACTCT TTCATGAAGC TAGAGAAAGG GCATCCAAAG CTCTTGGTTT TGCTAAAATG 
CTGAGGAAGG ATCTTGAAAT AGCAGCAGAG TTTGCATTGT CAGCCCCTGT GCGAGATTTT 
CTGAATGCTC TGAAAACAAA AGAGTATGTG AAGGTGCAAA TCCCTGGACT TGAAAACTTA 
CAAGTATTTG TCCCAAATAG TATTGCAGGG GAAAAATCTG TGATTTTACA GCTCCTAAAT 
GCAGCTGCAG GAAAGGATTG CTCGAAGGAT TCGGATGAGG TTGCATATGA GGCCTATTTA 
CTAATGACCA AACACAGTGA CAAAGACCAC GAATTAGACA ACAGTTGGAG CGCGTGGGAG 
GGTCAGCCAG TCAAAGTAGT GCCACAGGTG GAAACTGCTG ATACACTGCG AAGCATGCAG 
GTTGATAACC TCCTCCTTGT AGTCATTCAA TCTGCCCATC TTGTGAGTCA GAGAAAAGCT 
TTCCAACAGT CTATTGAAGG GCTCATATCT TTGCACCACG AACAGACATC CAGCCAACCA 
GTCATTGCCA AAGCTCTGCA ACAGCTAAAG AGTGATGCAT TACAGCTATG CAATAAGATA 
AGCAGTGCCA TTGATAGAGT TGATCGCATG TTCACATCTG AGTTTGATGC TGAAGTTGAT 
GAATCTGAAT CTGCCACTTT GCAACAATAC TACCGGGAAG CCATGATTCA GGGTTATAAT 
TTTGGATTTG AGTACCATAA AGAAGTTGTT CGCCTAATGT CTGGAGAATT CAGGCAGAAA 
ATTGGAGATA AGTATATCAG CTTTGCTAGA AAATGGATGA ATTATGTGCT AACAAAGTGT 
GAGAGTGGCC GAGGCACTCG ACCCAGGTGG GCAACTCAAG GTTTTGATTT TCTTCAAGCC 
ATTGAGCCAG CATTTATTTC AGCTCTCCCA GAAGATGACT TCTTGAGTTT ACAAGCTCTG 
ATGAATGAAT GCATTGGCCA TGTTATTGGA AAACCTCATA GTCCTGTTAC AGCTATTCAT 
CGAAACAGTC CCCGTCCTGT AAAGGTACCT CGATGCCATA GTGATCCACC AAATCCTCAT 
CTTATCATCC CCACTCCTGA AGGTTTCAGG GGTTCCAGTG TCCCTGAAAA TGATCGACTG 
GCTTCAATAG CAGCTGAGTT GCAGTTCAGA TCCCTTAGTC GTCACTCCAG TCCCACTGAA 
GAGCGAGAAG AACCATCTTA TCCAAAAGGA GATTCAGGTG GATCATCCCG GCGGAGTTGG 
GAGCTCAGAA CTTTAATCAA CCAGACCAAA GACACTGCTT CCAAGCAAGC GCCAATCGAA 
GCTGTTCAGA AGTCTGTTCA GTTGTTTGAA GAAAGGAGAT ACAGAGAGAT GAGGAGGAAG 
AACATCATTG GCCAAGTTTG TGACACCCCA AAATCTTATG ATAATGTCAT GCATGTAGGT 
TTGAGGAAAG TGACTTTCAA GTGGCAGAGA GGAAACAAAA TTGGTGAAGG CCAGTACGGA 
AAAGTCTACA CCTGTATCAG TGTTGACACT GGTGAACTGA TGGCTATGAA AGAAATAAGA 
TTTCAACCCA ATGATCACAA AACCATCAAA GAAACAGCAG ATGAACTGAA GATTTTTGAA 
GGCATCAAAC ACCCTAATCT TGTTCGTTAC TTTGGTGTGG AGCTCCATAG GGAAGAAATG 
TACATCTTCA TGGAGTACTG TGATGAGGGC ACCCTGGAGG AGGTATCAAA ACTGGGCCTT 
CAGGAACATG TCATTAGGCT CTACTCAAAG CAAATCACAA CTGCTATCAA TGTACTGCAT 
GAACATGGTA TTGTTCATCG AGACATTAAA GGAGCCAACA TCTTTCTTAC CTCATCTGGA 
CTGATTAAAT TAGGAGACTT TGGCTGCTCA GTGAAATTAA AGAATAACAC CCAGACAATG 
CCTGGAGAAG TGAACAGTAC TTTGGGGACT GCAGCATACA TGGCTCCAGA AGTCATTACT 
AGAGCTAAAG GAGAAGGGCA TGGGCGTGCA GCAGACATCT GGAGCCTGGG CTGTGTTGTA 
ATAGAGATGG TCACTGGCAA GAGGCCGTGG CATGAATACG AGCACAACTT CCAGATCATG 
TATAGAGTGG GGATGGGTCA TAAACCTCCT ATTCCTGACA AGGTGAGCCC AGAAGGGAAA 
GACTTCCTTT GCCACTGTCT GGAAAGTGAC CCAAAGATGA GGTGGACGGC TAGCCAGCTC 
CTCGACCATC CCTTTGTCAA GGTTTGCACA GATGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MAP3K4
Entrez Gene ID	106486799
Full Name	mitogen-activated protein kinase kinase kinase 4
Gene Type	protein-coding
Organism	Apteryx australis mantelli

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MAP3K4 cDNA ORF clone, Apteryx australis mantelli

Gene

Clones

gRNAs

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