Apid22 cDNA ORF clone, Apis mellifera(honey bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	Apid22
Entrez Gene ID	494510
Full Name	apidaecin type 22
Gene Type	protein-coding
Organism	Apis mellifera(honey bee)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001011642.1	NP_001011642.1	apidaecins type 22 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Apidaecin multipeptide precursor structure: a putative mechanism for amplification of the insect antibacterial response.
Casteels-Josson K, Capaci T, Casteels P, Tempst P
The EMBO journal12(4)1569-78(1993 Apr)

Apidaecins: antibacterial peptides from honeybees.
Casteels P, Ampe C, Jacobs F, Vaeck M, Tempst P
The EMBO journal8(8)2387-91(1989 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Apid22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Apid22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHd00463	NM_001011642.1 Latest version!	Apis mellifera apidaecin type 22 (Apid22), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Apid22 ( NM_001011642.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> NP_001011642.1 Apis mellifera apidaecin type 22 (Apid22), mRNA.

Price & Availability↑

CloneID	OHd00463
Clone ID Related Accession (Same CDS sequence)	NM_001011642.1
Accession Version	NM_001011642.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Apis mellifera(honey bee)
Product	apidaecins type 22 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X72576.1. ##Evidence-Data-START## Transcript exon combination :: X72576.1 [ECO:0000332] ##Evidence-Data-END##

ATGAAGAATT TTGCCTTAGC AATTCTTGTT GTTACCTTTG TAGTCGCGGT ATTTGGGAAT 
ACCAACCTAG ATCCGCCTAC TCGACCTACG CGCCTTCGCC GTGAAGCTGA ACCGGAAGCT 
GAACCCGGCA ATAATCGGCC AGTATATATT CCACAACCAA GACCACCTCA TCCGCGTCTT 
CGCCGTGAAG CTGAACCGGA AGCTGAACCC GGCAATAATC GACCAGTATA TATTCCACAA 
CCAAGGCCAC CTCATCCGCG TCTTCGCCGT GAAGCTGAAC CGGAAGCTGA ACCCGGCAAT 
AATCGGCCAG TATATATTCC ACAACCAAGG CCACCTCATC CGCGTCTTCG CCGTGAAGCT 
GAACCGGAAG CTGAACCCGG CAATAATCGG CCAGTATATA TTCCACAACC AAGACCACCT 
CATCCGCGAA TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001011642.1
CDS	20..454
Translation	MKNFALAILVVTFVVAVFGNTNLDPPTRPTRLRREAEPEAEPGNNRPVYIPQPRPPHPRLRREAEPEAEPGNNRPVYIPQPRPPHPRLRREAEPEAEPGNNRPVYIPQPRPPHPRLRREAEPEAEPGNNRPVYIPQPRPPHPRI

Target ORF information:

RefSeq Version	NM_001011642.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera apidaecin type 22 (Apid22), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001011642.1

ORF Insert Sequence:

ATGAAGAATT TTGCCTTAGC AATTCTTGTT GTTACCTTTG TAGTCGCGGT ATTTGGGAAT 
ACCAACCTAG ATCCGCCTAC TCGACCTACG CGCCTTCGCC GTGAAGCTGA ACCGGAAGCT 
GAACCCGGCA ATAATCGGCC AGTATATATT CCACAACCAA GACCACCTCA TCCGCGTCTT 
CGCCGTGAAG CTGAACCGGA AGCTGAACCC GGCAATAATC GACCAGTATA TATTCCACAA 
CCAAGGCCAC CTCATCCGCG TCTTCGCCGT GAAGCTGAAC CGGAAGCTGA ACCCGGCAAT 
AATCGGCCAG TATATATTCC ACAACCAAGG CCACCTCATC CGCGTCTTCG CCGTGAAGCT 
GAACCGGAAG CTGAACCCGG CAATAATCGG CCAGTATATA TTCCACAACC AAGACCACCT 
CATCCGCGAA TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Apidaecin multipeptide precursor structure: a putative mechanism for amplification of the insect antibacterial response.
The EMBO journal12(4)1569-78(1993 Apr)
Casteels-Josson K,Capaci T,Casteels P,Tempst P

Apidaecins: antibacterial peptides from honeybees.
The EMBO journal8(8)2387-91(1989 Aug)
Casteels P,Ampe C,Jacobs F,Vaeck M,Tempst P