Apid1 cDNA ORF clone, Apis mellifera(honey bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	Apid1
Entrez Gene ID	406140
Full Name	apidaecin 1
Synonyms	Apid14,Apid22,GB13473
Gene Type	protein-coding
Organism	Apis mellifera(honey bee)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001011613.1	NP_001011613.1	apidaecins type 14 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Identification of New Resistance Mechanisms in Escherichia coli against Apidaecin 1b Using Quantitative Gel- and LC-MS-Based Proteomics.
Schmidt R, Krizsan A, Volke D, Knappe D, Hoffmann R
Journal of proteome research15(8)2607-17(2016 08)

Finding the missing honey bee genes: lessons learned from a genome upgrade.
Elsik CG, Worley KC, Bennett AK, Beye M, Camara F, Childers CP, de Graaf DC, Debyser G, Deng J, Devreese B, Elhaik E, Evans JD, Foster LJ, Graur D, Guigo R, , Hoff KJ, Holder ME, Hudson ME, Hunt GJ, Jiang H, Joshi V, Khetani RS, Kosarev P, Kovar CL, Ma J, Maleszka R, Moritz RF, Munoz-Torres MC, Murphy TD, Muzny DM, Newsham IF, Reese JT, Robertson HM, Robinson GE, Rueppell O, Solovyev V, Stanke M, Stolle E, Tsuruda JM, Vaerenbergh MV, Waterhouse RM, Weaver DB, Whitfield CW, Wu Y, Zdobnov EM, Zhang L, Zhu D, Gibbs RA,
BMC genomics1586(2014 Jan)

Insights into social insects from the genome of the honeybee Apis mellifera.

Nature443(7114)931-49(2006 Oct)

Apidaecin multipeptide precursor structure: a putative mechanism for amplification of the insect antibacterial response.
Casteels-Josson K, Capaci T, Casteels P, Tempst P
The EMBO journal12(4)1569-78(1993 Apr)

Apidaecins: antibacterial peptides from honeybees.
Casteels P, Ampe C, Jacobs F, Vaeck M, Tempst P
The EMBO journal8(8)2387-91(1989 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

induced apidaecin resistances by incubating Escherichia coli with increasing concentrations of apidaecin 1b isolated from honeybees, and quantitatively evaluated the EColi proteome differences between wild-type and apidaecin resistant strains.
Title: Identification of New Resistance Mechanisms in Escherichia coli against Apidaecin 1b Using Quantitative Gel- and LC-MS-Based Proteomics.

The following Apid1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Apid1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHd00133	NM_001011613.1 Latest version!	Apis mellifera apidaecin 1 (Apid1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Apid1 ( NM_001011613.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> NP_001011613.1 Apis mellifera apidaecin 1 (Apid1), mRNA.

Price & Availability↑

CloneID	OHd00133
Clone ID Related Accession (Same CDS sequence)	NM_001011613.1
Accession Version	NM_001011613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Apis mellifera(honey bee)
Product	apidaecins type 14 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X72575.1. On Feb 5, 2005 this sequence version replaced XM_393556.1. ##Evidence-Data-START## Transcript exon combination :: X72575.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00120372, SAMN00996631 [ECO:0000348] ##Evidence-Data-END##

ATGAAGAATT TTGCCTTAGC AATTCTTGTT GTTACCTTTG TAGTCGCGGT ATTTGGGAAT 
ACCAACCTAG ATCCGCCTAC TCGACCTGCG CGCCTTCGCC GTGAAGCTAA ACCGGAAGCT 
GAACCCGGCA ATAATCGGCC AATATATATT CCACAACCAA GACCACCTCA TCCGCGCCTT 
CGCCGTGAAG CTGAACCGAA AGCTGAACCC GGCAATAATC GGCCAATATA TATTCCACAA 
CCAAGACCAC CTCATCCGCG TCTTCGCCGT GAAGCTGAAT CGGAAGCTGA ACCCGGCAAT 
AATCGACCAG TATATATTCC ACAACCGAGA CCACCTCATC CGCGTCTTCG CCGTGAACCG 
GAAGCTGAAC CCGGCAATAA TCGGCCAGTA TATATTCCAC AACCGAGACC ACCTCATCCG 
CGTCTTCGCC GTGAACCGGA AGCTGAACCC GGCAATAATC GGCCAGTATA TATTCCACAA 
CCAAGACCAC CTCATCCGCG AATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001011613.1
CDS	49..555
Translation	MKNFALAILVVTFVVAVFGNTNLDPPTRPARLRREAKPEAEPGNNRPIYIPQPRPPHPRLRREAEPKAEPGNNRPIYIPQPRPPHPRLRREAESEAEPGNNRPVYIPQPRPPHPRLRREPEAEPGNNRPVYIPQPRPPHPRLRREPEAEPGNNRPVYIPQPRPPHPRI

Target ORF information:

RefSeq Version	NM_001011613.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera apidaecin 1 (Apid1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001011613.1

ORF Insert Sequence:

ATGAAGAATT TTGCCTTAGC AATTCTTGTT GTTACCTTTG TAGTCGCGGT ATTTGGGAAT 
ACCAACCTAG ATCCGCCTAC TCGACCTGCG CGCCTTCGCC GTGAAGCTAA ACCGGAAGCT 
GAACCCGGCA ATAATCGGCC AATATATATT CCACAACCAA GACCACCTCA TCCGCGCCTT 
CGCCGTGAAG CTGAACCGAA AGCTGAACCC GGCAATAATC GGCCAATATA TATTCCACAA 
CCAAGACCAC CTCATCCGCG TCTTCGCCGT GAAGCTGAAT CGGAAGCTGA ACCCGGCAAT 
AATCGACCAG TATATATTCC ACAACCGAGA CCACCTCATC CGCGTCTTCG CCGTGAACCG 
GAAGCTGAAC CCGGCAATAA TCGGCCAGTA TATATTCCAC AACCGAGACC ACCTCATCCG 
CGTCTTCGCC GTGAACCGGA AGCTGAACCC GGCAATAATC GGCCAGTATA TATTCCACAA 
CCAAGACCAC CTCATCCGCG AATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of New Resistance Mechanisms in Escherichia coli against Apidaecin 1b Using Quantitative Gel- and LC-MS-Based Proteomics.
Journal of proteome research15(8)2607-17(2016 08)
Schmidt R,Krizsan A,Volke D,Knappe D,Hoffmann R

Finding the missing honey bee genes: lessons learned from a genome upgrade.
BMC genomics1586(2014 Jan)
Elsik CG,Worley KC,Bennett AK,Beye M,Camara F,Childers CP,de Graaf DC,Debyser G,Deng J,Devreese B,Elhaik E,Evans JD,Foster LJ,Graur D,Guigo R,,Hoff KJ,Holder ME,Hudson ME,Hunt GJ,Jiang H,Joshi V,Khetani RS,Kosarev P,Kovar CL,Ma J,Maleszka R,Moritz RF,Munoz-Torres MC,Murphy TD,Muzny DM,Newsham IF,Reese JT,Robertson HM,Robinson GE,Rueppell O,Solovyev V,Stanke M,Stolle E,Tsuruda JM,Vaerenbergh MV,Waterhouse RM,Weaver DB,Whitfield CW,Wu Y,Zdobnov EM,Zhang L,Zhu D,Gibbs RA,

Insights into social insects from the genome of the honeybee Apis mellifera.
Nature443(7114)931-49(2006 Oct)

Apidaecin multipeptide precursor structure: a putative mechanism for amplification of the insect antibacterial response.
The EMBO journal12(4)1569-78(1993 Apr)
Casteels-Josson K,Capaci T,Casteels P,Tempst P

Apidaecins: antibacterial peptides from honeybees.
The EMBO journal8(8)2387-91(1989 Aug)
Casteels P,Ampe C,Jacobs F,Vaeck M,Tempst P