LOC108004236 cDNA ORF clone, Apis cerana(Asiatic honeybee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC108004236
Entrez Gene ID	108004236
Full Name	bumetanide-sensitive sodium-(potassium)-chloride cotransporter
Gene Type	protein-coding
Organism	Apis cerana(Asiatic honeybee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017066995.1	XP_016922484.1	bumetanide-sensitive sodium-(potassium)-chloride cotransporter
XM_017066993.1	XP_016922482.1	bumetanide-sensitive sodium-(potassium)-chloride cotransporter
XM_017066994.2	XP_016922483.1	bumetanide-sensitive sodium-(potassium)-chloride cotransporter
XM_017066994.1	XP_016922483.1	bumetanide-sensitive sodium-(potassium)-chloride cotransporter
XM_017066995.2	XP_016922484.1	bumetanide-sensitive sodium-(potassium)-chloride cotransporter
XM_017066993.2	XP_016922482.1	bumetanide-sensitive sodium-(potassium)-chloride cotransporter

Pathways from BioSystems
Gene Ontology

The following LOC108004236 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC108004236 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAp30532	XM_017066993.2 Latest version!	Apis cerana bumetanide-sensitive sodium-(potassium)-chloride cotransporter (LOC108004236), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAp30532	XM_017066994.2 Latest version!	Apis cerana bumetanide-sensitive sodium-(potassium)-chloride cotransporter (LOC108004236), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAp30532	XM_017066995.2 Latest version!	Apis cerana bumetanide-sensitive sodium-(potassium)-chloride cotransporter (LOC108004236), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OAp30532	XM_017066994.1	Apis cerana bumetanide-sensitive sodium-(potassium)-chloride cotransporter (LOC108004236), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAp30532	XM_017066995.1	Apis cerana bumetanide-sensitive sodium-(potassium)-chloride cotransporter (LOC108004236), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAp30532	XM_017066993.1	Apis cerana bumetanide-sensitive sodium-(potassium)-chloride cotransporter (LOC108004236), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC108004236 ( XM_017066995.1 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_016922484.1 Apis cerana bumetanide-sensitive sodium-(potassium)-chloride cotransporter (LOC108004236), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OAp30532
Clone ID Related Accession (Same CDS sequence)	XM_017066995.1 , XM_017066993.1 , XM_017066994.2 , XM_017066994.1 , XM_017066995.2 , XM_017066993.2
Accession Version	XM_017066995.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-08
Organism	Apis cerana(Asiatic honeybee)
Product	bumetanide-sensitive sodium-(potassium)-chloride cotransporter
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016017734.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis cerana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACATG AAAATGGTAT AAGCGGTGAT ACCGATTCCG AAACGGTAGA ATTGAATCCA 
TTGAGAAGAA CCAGATTCCA TGTAAATCGA GTGGACAGTC TTGAAGGTCG AGCGAGCCTT 
CTTGGAGAAC AAGAAACGAG AAAGTCCCTC CGACACATGA CTAGGGAGGC ACTGCCTAGA 
CTGGATAATT ATAGAAACAT TATGAGTATC CAGGCCGCTC ATAGACCGTC TTTGGACGAG 
CTTCACAATC CCACTGTGCT TAACAAGGGT GCAGGATCGC ATAATTTGCC ACCAGTGCAA 
GTGAAGCCAC CGACTTCCGG AATAAAATTC GGATGGATTC AAGGAGTTTT ATTAAGATGC 
CTTCTCAATA TTTGGGGGGT AATGCTTTTT TTACGTCTCT CCTGGGTCGT AGCACAAGCA 
GGCATAGGAG AAGCTATTTT ATTGATTTTA ACAACGACAA CTGTGACTAC GATAACATCG 
TTATCTATGT CGGCAATTAG CACAAATGGT CTCATTAAAG GAGGTGGCAC GTATTACATG 
ATTTCCAGAT CACTGGGGCC AGAATTCGGA GGATCTATAG GACTTATATT TTCTTTGGCG 
AACGCGGTCG CTTGTTCCAT GTATGTTGTT GGATTTTGCG AGAGTATGGT CGATTGCTTA 
AAATCAAATG GAGTTTGTAT TGTTGATTGC GACAATACGG ATATTAGAAT TATAGGTTGT 
ATCACGATAG TTCTGTTACT TTTGATCGTT ATGATTGGAT TGGAATGGGA AGCCAAAGCT 
CAAATTGGTT TGCTGATTAT ATTGCTTTTA GCTATCGCTG ATTTTATGAT CGGCACCTTT 
ATTGGACCTA AAGATGATCA AGAACGGGCT AAAGGATTTA TGGGATATAA TGCTGATCTA 
TTCAAGGAGA ACTTTTATCC GGATTATAGA TATAGTGAGG GAATGAAGCA TAATTTTTTT 
TCAGTCTTGG CTATTTTTTT CCCTGCGGCT ACAGGTATCC TGGCAGGTGC GAATATATCC 
GGTGATTTGA AGGATCCGCA AACAGCTATT CCTAAAGGAA CATTGTTAGC TATTCTTTTA 
ACAACTATAT CTTACCTCTT TATGGCACTT ATGGTGGGCG GCTCAGTTAT GCGAGATGCA 
TCTGGCGATG TTAATGATCT GTGGAACATT TTCAATAGTT CATATACTGC CTTATCAAGT 
TCGAATACGA CTAATGAAAC AACTGAATCT TCAATTATTG TAAATTCCAA TCAAATAGTA 
TGGAGTATAT ATAGTACAAA ATTTAATTGT ACAGGAAATT GTCAATATGG TAGCCATAAT 
AGTTTTCAGG TGATCGAATT GGTTTCTGCA TTTGGACCTT TTATTTATGC TGGATGTTTT 
GCAGCTACTT TATCAAGCGC TTTAGCATCA TTAGTTTCCG CTCCCAAAGT ATTTCAGGCA 
CTTTGTCTTG ATAAATTATA TCCTGGAATA GCATGGTTTA GTGGCGACAA AGATAAAGAA 
CCAATTCGTG GTTATCTTCT TACTTTTATA ATCGCAGTTG GTTTTATTTT AATCGGAGAA 
TTGAATGCTA TAGCTCCTTT GATCTCAAAT TTTTTCTTGG CTGCCTATAC TCTTATTAAT 
TTTAGCACTT TTCATGCTTC GTTGGCAAAA CCAATTGGTT GGAGACCAAC GTTTAAATAT 
TATAATATGT GGCTAAGTCT TGCTGGTGCT ATTCTCTGTG TTTCTGTGAT GTTCTTGATC 
TCATGGTGGA CAGCGTTGAT CACATTATTT GTAGTATTAA CACTTTATTT AATAGTTTCT 
TATAGAAAAC CAGATGTTAA TTGGGGTTCT ACAACACAAG CCCAAACATA TAATAATGCC 
TTAAATGCTG TTCAACAATT AGATAGAGTT GAAGAACATG TTAAAAATTA TAGACCTCAA 
TTGTTAGTAT TAAGTGGCAC TCCAAGTGCA AGATCAGCAC TTATTGATTT TGCACATCAT 
ATTACTAAAA ATAATAGTTT ATTTATTTGT GGCCATATTA TTGAGACACC AATATCATAT 
AAGACGCGAA ATTCCATGAT TGCGAATTGT ACTTCCTGGT TTAGAGCAAA TAAAATTAAG 
GCATTTTATT CTCTGGTGGA TGGTGCAAAT TTTCAAGACG GCACTACTTC TCTTTTACAA 
GCTGCCGGTT TAGGAAAAAT GAAGCCTAAT ATTTTATTAA TGGGCTATAA GCAAGACTGG 
GCAACTTGTC CTCGAGAAAA TTTAAATATG TACTTTAATA TAATGCACAA AGCTTTAGAT 
ATGCATATAG CAGTAGCTCT TCTTCGAATA AGTGAAGGTT TAGATTGTAG TATAGGAGAT 
GTTGAAGAAC AAAAGAAAAG TATCCAAACA ATTCCAGGGA ATCAAAGCTT TTCTCAACTT 
AGCCAAGCCA GTAGTACATC AGATATTTCT ATTCCTGGAA GTCCAGCTCC AAGAAGATCT 
AAAATGATTA ATGAATATCC TAATCCAACT GCAGAAGATC AACGTGAAAA TCGACAAAAT 
TTCTTACCCA TAGCGAAAGA TATACTTAAT GCTGTAACAA AATTTCAACG TAAACAAAAA 
AAGGGTACTA TAGATGTATG GTGGTTGTAT GATGATGGTG GTTTAACTCT TTTATTACCT 
TATATTATCA GTACAAGACG TAATTGGTCT AATAATAAAT TAAGAGTTTT TGCTTTAGCA 
AATAAAAATT CTGAACTCGA ATATGAACAA AGAAATATGG CAAGTCTTTT ATCGAAATTC 
AGGATAGATT ATTCTGCATT GAAAGTTATA CCAGATATTT CAAAACCAGC ACAGAATACT 
ACCAAAAGTT TCTTTGATTC ATTAATAGCA AATTTCCAAG AAACAGAAAA TACAAAAACA 
ATGAATGATG ATATTATCAA AGATTCTGAA TTAATTGCTA TGAAAGAAAA AACAAATAGA 
CATCTACGTT TAAGAGAATT ATTACTTGAA AATTCTATGG ACGCTAATTT AGTAGTTATG 
ACATTGCCAA TGCCTCGAAA GGGTGCTGTT TCAGCACCTC TCTATATGGC TTGGCTCGAA 
ACTTTAACGC GTGATATGCC ACCATTTTTA CTTGTTCGAG GAAATCACAC ATCTGTTTTA 
ACTTTTTACT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016922484.1
CDS	507..3641
Translation	MAHENGISGDTDSETVELNPLRRTRFHVNRVDSLEGRASLLGEQETRKSLRHMTREALPRLDNYRNIMSIQAAHRPSLDELHNPTVLNKGAGSHNLPPVQVKPPTSGIKFGWIQGVLLRCLLNIWGVMLFLRLSWVVAQAGIGEAILLILTTTTVTTITSLSMSAISTNGLIKGGGTYYMISRSLGPEFGGSIGLIFSLANAVACSMYVVGFCESMVDCLKSNGVCIVDCDNTDIRIIGCITIVLLLLIVMIGLEWEAKAQIGLLIILLLAIADFMIGTFIGPKDDQERAKGFMGYNADLFKENFYPDYRYSEGMKHNFFSVLAIFFPAATGILAGANISGDLKDPQTAIPKGTLLAILLTTISYLFMALMVGGSVMRDASGDVNDLWNIFNSSYTALSSSNTTNETTESSIIVNSNQIVWSIYSTKFNCTGNCQYGSHNSFQVIELVSAFGPFIYAGCFAATLSSALASLVSAPKVFQALCLDKLYPGIAWFSGDKDKEPIRGYLLTFIIAVGFILIGELNAIAPLISNFFLAAYTLINFSTFHASLAKPIGWRPTFKYYNMWLSLAGAILCVSVMFLISWWTALITLFVVLTLYLIVSYRKPDVNWGSTTQAQTYNNALNAVQQLDRVEEHVKNYRPQLLVLSGTPSARSALIDFAHHITKNNSLFICGHIIETPISYKTRNSMIANCTSWFRANKIKAFYSLVDGANFQDGTTSLLQAAGLGKMKPNILLMGYKQDWATCPRENLNMYFNIMHKALDMHIAVALLRISEGLDCSIGDVEEQKKSIQTIPGNQSFSQLSQASSTSDISIPGSPAPRRSKMINEYPNPTAEDQRENRQNFLPIAKDILNAVTKFQRKQKKGTIDVWWLYDDGGLTLLLPYIISTRRNWSNNKLRVFALANKNSELEYEQRNMASLLSKFRIDYSALKVIPDISKPAQNTTKSFFDSLIANFQETENTKTMNDDIIKDSELIAMKEKTNRHLRLRELLLENSMDANLVVMTLPMPRKGAVSAPLYMAWLETLTRDMPPFLLVRGNHTSVLTFYS

Target ORF information:

RefSeq Version	XM_017066995.1
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana bumetanide-sensitive sodium-(potassium)-chloride cotransporter (LOC108004236), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017066995.1

ORF Insert Sequence:

ATGGCACATG AAAATGGTAT AAGCGGTGAT ACCGATTCCG AAACGGTAGA ATTGAATCCA 
TTGAGAAGAA CCAGATTCCA TGTAAATCGA GTGGACAGTC TTGAAGGTCG AGCGAGCCTT 
CTTGGAGAAC AAGAAACGAG AAAGTCCCTC CGACACATGA CTAGGGAGGC ACTGCCTAGA 
CTGGATAATT ATAGAAACAT TATGAGTATC CAGGCCGCTC ATAGACCGTC TTTGGACGAG 
CTTCACAATC CCACTGTGCT TAACAAGGGT GCAGGATCGC ATAATTTGCC ACCAGTGCAA 
GTGAAGCCAC CGACTTCCGG AATAAAATTC GGATGGATTC AAGGAGTTTT ATTAAGATGC 
CTTCTCAATA TTTGGGGGGT AATGCTTTTT TTACGTCTCT CCTGGGTCGT AGCACAAGCA 
GGCATAGGAG AAGCTATTTT ATTGATTTTA ACAACGACAA CTGTGACTAC GATAACATCG 
TTATCTATGT CGGCAATTAG CACAAATGGT CTCATTAAAG GAGGTGGCAC GTATTACATG 
ATTTCCAGAT CACTGGGGCC AGAATTCGGA GGATCTATAG GACTTATATT TTCTTTGGCG 
AACGCGGTCG CTTGTTCCAT GTATGTTGTT GGATTTTGCG AGAGTATGGT CGATTGCTTA 
AAATCAAATG GAGTTTGTAT TGTTGATTGC GACAATACGG ATATTAGAAT TATAGGTTGT 
ATCACGATAG TTCTGTTACT TTTGATCGTT ATGATTGGAT TGGAATGGGA AGCCAAAGCT 
CAAATTGGTT TGCTGATTAT ATTGCTTTTA GCTATCGCTG ATTTTATGAT CGGCACCTTT 
ATTGGACCTA AAGATGATCA AGAACGGGCT AAAGGATTTA TGGGATATAA TGCTGATCTA 
TTCAAGGAGA ACTTTTATCC GGATTATAGA TATAGTGAGG GAATGAAGCA TAATTTTTTT 
TCAGTCTTGG CTATTTTTTT CCCTGCGGCT ACAGGTATCC TGGCAGGTGC GAATATATCC 
GGTGATTTGA AGGATCCGCA AACAGCTATT CCTAAAGGAA CATTGTTAGC TATTCTTTTA 
ACAACTATAT CTTACCTCTT TATGGCACTT ATGGTGGGCG GCTCAGTTAT GCGAGATGCA 
TCTGGCGATG TTAATGATCT GTGGAACATT TTCAATAGTT CATATACTGC CTTATCAAGT 
TCGAATACGA CTAATGAAAC AACTGAATCT TCAATTATTG TAAATTCCAA TCAAATAGTA 
TGGAGTATAT ATAGTACAAA ATTTAATTGT ACAGGAAATT GTCAATATGG TAGCCATAAT 
AGTTTTCAGG TGATCGAATT GGTTTCTGCA TTTGGACCTT TTATTTATGC TGGATGTTTT 
GCAGCTACTT TATCAAGCGC TTTAGCATCA TTAGTTTCCG CTCCCAAAGT ATTTCAGGCA 
CTTTGTCTTG ATAAATTATA TCCTGGAATA GCATGGTTTA GTGGCGACAA AGATAAAGAA 
CCAATTCGTG GTTATCTTCT TACTTTTATA ATCGCAGTTG GTTTTATTTT AATCGGAGAA 
TTGAATGCTA TAGCTCCTTT GATCTCAAAT TTTTTCTTGG CTGCCTATAC TCTTATTAAT 
TTTAGCACTT TTCATGCTTC GTTGGCAAAA CCAATTGGTT GGAGACCAAC GTTTAAATAT 
TATAATATGT GGCTAAGTCT TGCTGGTGCT ATTCTCTGTG TTTCTGTGAT GTTCTTGATC 
TCATGGTGGA CAGCGTTGAT CACATTATTT GTAGTATTAA CACTTTATTT AATAGTTTCT 
TATAGAAAAC CAGATGTTAA TTGGGGTTCT ACAACACAAG CCCAAACATA TAATAATGCC 
TTAAATGCTG TTCAACAATT AGATAGAGTT GAAGAACATG TTAAAAATTA TAGACCTCAA 
TTGTTAGTAT TAAGTGGCAC TCCAAGTGCA AGATCAGCAC TTATTGATTT TGCACATCAT 
ATTACTAAAA ATAATAGTTT ATTTATTTGT GGCCATATTA TTGAGACACC AATATCATAT 
AAGACGCGAA ATTCCATGAT TGCGAATTGT ACTTCCTGGT TTAGAGCAAA TAAAATTAAG 
GCATTTTATT CTCTGGTGGA TGGTGCAAAT TTTCAAGACG GCACTACTTC TCTTTTACAA 
GCTGCCGGTT TAGGAAAAAT GAAGCCTAAT ATTTTATTAA TGGGCTATAA GCAAGACTGG 
GCAACTTGTC CTCGAGAAAA TTTAAATATG TACTTTAATA TAATGCACAA AGCTTTAGAT 
ATGCATATAG CAGTAGCTCT TCTTCGAATA AGTGAAGGTT TAGATTGTAG TATAGGAGAT 
GTTGAAGAAC AAAAGAAAAG TATCCAAACA ATTCCAGGGA ATCAAAGCTT TTCTCAACTT 
AGCCAAGCCA GTAGTACATC AGATATTTCT ATTCCTGGAA GTCCAGCTCC AAGAAGATCT 
AAAATGATTA ATGAATATCC TAATCCAACT GCAGAAGATC AACGTGAAAA TCGACAAAAT 
TTCTTACCCA TAGCGAAAGA TATACTTAAT GCTGTAACAA AATTTCAACG TAAACAAAAA 
AAGGGTACTA TAGATGTATG GTGGTTGTAT GATGATGGTG GTTTAACTCT TTTATTACCT 
TATATTATCA GTACAAGACG TAATTGGTCT AATAATAAAT TAAGAGTTTT TGCTTTAGCA 
AATAAAAATT CTGAACTCGA ATATGAACAA AGAAATATGG CAAGTCTTTT ATCGAAATTC 
AGGATAGATT ATTCTGCATT GAAAGTTATA CCAGATATTT CAAAACCAGC ACAGAATACT 
ACCAAAAGTT TCTTTGATTC ATTAATAGCA AATTTCCAAG AAACAGAAAA TACAAAAACA 
ATGAATGATG ATATTATCAA AGATTCTGAA TTAATTGCTA TGAAAGAAAA AACAAATAGA 
CATCTACGTT TAAGAGAATT ATTACTTGAA AATTCTATGG ACGCTAATTT AGTAGTTATG 
ACATTGCCAA TGCCTCGAAA GGGTGCTGTT TCAGCACCTC TCTATATGGC TTGGCTCGAA 
ACTTTAACGC GTGATATGCC ACCATTTTTA CTTGTTCGAG GAAATCACAC ATCTGTTTTA 
ACTTTTTACT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108004236 ( XM_017066993.1 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_016922482.1 Apis cerana bumetanide-sensitive sodium-(potassium)-chloride cotransporter (LOC108004236), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAp30532
Clone ID Related Accession (Same CDS sequence)	XM_017066995.1 , XM_017066993.1 , XM_017066994.2 , XM_017066994.1 , XM_017066995.2 , XM_017066993.2
Accession Version	XM_017066993.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-08
Organism	Apis cerana(Asiatic honeybee)
Product	bumetanide-sensitive sodium-(potassium)-chloride cotransporter
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016017734.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis cerana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACATG AAAATGGTAT AAGCGGTGAT ACCGATTCCG AAACGGTAGA ATTGAATCCA 
TTGAGAAGAA CCAGATTCCA TGTAAATCGA GTGGACAGTC TTGAAGGTCG AGCGAGCCTT 
CTTGGAGAAC AAGAAACGAG AAAGTCCCTC CGACACATGA CTAGGGAGGC ACTGCCTAGA 
CTGGATAATT ATAGAAACAT TATGAGTATC CAGGCCGCTC ATAGACCGTC TTTGGACGAG 
CTTCACAATC CCACTGTGCT TAACAAGGGT GCAGGATCGC ATAATTTGCC ACCAGTGCAA 
GTGAAGCCAC CGACTTCCGG AATAAAATTC GGATGGATTC AAGGAGTTTT ATTAAGATGC 
CTTCTCAATA TTTGGGGGGT AATGCTTTTT TTACGTCTCT CCTGGGTCGT AGCACAAGCA 
GGCATAGGAG AAGCTATTTT ATTGATTTTA ACAACGACAA CTGTGACTAC GATAACATCG 
TTATCTATGT CGGCAATTAG CACAAATGGT CTCATTAAAG GAGGTGGCAC GTATTACATG 
ATTTCCAGAT CACTGGGGCC AGAATTCGGA GGATCTATAG GACTTATATT TTCTTTGGCG 
AACGCGGTCG CTTGTTCCAT GTATGTTGTT GGATTTTGCG AGAGTATGGT CGATTGCTTA 
AAATCAAATG GAGTTTGTAT TGTTGATTGC GACAATACGG ATATTAGAAT TATAGGTTGT 
ATCACGATAG TTCTGTTACT TTTGATCGTT ATGATTGGAT TGGAATGGGA AGCCAAAGCT 
CAAATTGGTT TGCTGATTAT ATTGCTTTTA GCTATCGCTG ATTTTATGAT CGGCACCTTT 
ATTGGACCTA AAGATGATCA AGAACGGGCT AAAGGATTTA TGGGATATAA TGCTGATCTA 
TTCAAGGAGA ACTTTTATCC GGATTATAGA TATAGTGAGG GAATGAAGCA TAATTTTTTT 
TCAGTCTTGG CTATTTTTTT CCCTGCGGCT ACAGGTATCC TGGCAGGTGC GAATATATCC 
GGTGATTTGA AGGATCCGCA AACAGCTATT CCTAAAGGAA CATTGTTAGC TATTCTTTTA 
ACAACTATAT CTTACCTCTT TATGGCACTT ATGGTGGGCG GCTCAGTTAT GCGAGATGCA 
TCTGGCGATG TTAATGATCT GTGGAACATT TTCAATAGTT CATATACTGC CTTATCAAGT 
TCGAATACGA CTAATGAAAC AACTGAATCT TCAATTATTG TAAATTCCAA TCAAATAGTA 
TGGAGTATAT ATAGTACAAA ATTTAATTGT ACAGGAAATT GTCAATATGG TAGCCATAAT 
AGTTTTCAGG TGATCGAATT GGTTTCTGCA TTTGGACCTT TTATTTATGC TGGATGTTTT 
GCAGCTACTT TATCAAGCGC TTTAGCATCA TTAGTTTCCG CTCCCAAAGT ATTTCAGGCA 
CTTTGTCTTG ATAAATTATA TCCTGGAATA GCATGGTTTA GTGGCGACAA AGATAAAGAA 
CCAATTCGTG GTTATCTTCT TACTTTTATA ATCGCAGTTG GTTTTATTTT AATCGGAGAA 
TTGAATGCTA TAGCTCCTTT GATCTCAAAT TTTTTCTTGG CTGCCTATAC TCTTATTAAT 
TTTAGCACTT TTCATGCTTC GTTGGCAAAA CCAATTGGTT GGAGACCAAC GTTTAAATAT 
TATAATATGT GGCTAAGTCT TGCTGGTGCT ATTCTCTGTG TTTCTGTGAT GTTCTTGATC 
TCATGGTGGA CAGCGTTGAT CACATTATTT GTAGTATTAA CACTTTATTT AATAGTTTCT 
TATAGAAAAC CAGATGTTAA TTGGGGTTCT ACAACACAAG CCCAAACATA TAATAATGCC 
TTAAATGCTG TTCAACAATT AGATAGAGTT GAAGAACATG TTAAAAATTA TAGACCTCAA 
TTGTTAGTAT TAAGTGGCAC TCCAAGTGCA AGATCAGCAC TTATTGATTT TGCACATCAT 
ATTACTAAAA ATAATAGTTT ATTTATTTGT GGCCATATTA TTGAGACACC AATATCATAT 
AAGACGCGAA ATTCCATGAT TGCGAATTGT ACTTCCTGGT TTAGAGCAAA TAAAATTAAG 
GCATTTTATT CTCTGGTGGA TGGTGCAAAT TTTCAAGACG GCACTACTTC TCTTTTACAA 
GCTGCCGGTT TAGGAAAAAT GAAGCCTAAT ATTTTATTAA TGGGCTATAA GCAAGACTGG 
GCAACTTGTC CTCGAGAAAA TTTAAATATG TACTTTAATA TAATGCACAA AGCTTTAGAT 
ATGCATATAG CAGTAGCTCT TCTTCGAATA AGTGAAGGTT TAGATTGTAG TATAGGAGAT 
GTTGAAGAAC AAAAGAAAAG TATCCAAACA ATTCCAGGGA ATCAAAGCTT TTCTCAACTT 
AGCCAAGCCA GTAGTACATC AGATATTTCT ATTCCTGGAA GTCCAGCTCC AAGAAGATCT 
AAAATGATTA ATGAATATCC TAATCCAACT GCAGAAGATC AACGTGAAAA TCGACAAAAT 
TTCTTACCCA TAGCGAAAGA TATACTTAAT GCTGTAACAA AATTTCAACG TAAACAAAAA 
AAGGGTACTA TAGATGTATG GTGGTTGTAT GATGATGGTG GTTTAACTCT TTTATTACCT 
TATATTATCA GTACAAGACG TAATTGGTCT AATAATAAAT TAAGAGTTTT TGCTTTAGCA 
AATAAAAATT CTGAACTCGA ATATGAACAA AGAAATATGG CAAGTCTTTT ATCGAAATTC 
AGGATAGATT ATTCTGCATT GAAAGTTATA CCAGATATTT CAAAACCAGC ACAGAATACT 
ACCAAAAGTT TCTTTGATTC ATTAATAGCA AATTTCCAAG AAACAGAAAA TACAAAAACA 
ATGAATGATG ATATTATCAA AGATTCTGAA TTAATTGCTA TGAAAGAAAA AACAAATAGA 
CATCTACGTT TAAGAGAATT ATTACTTGAA AATTCTATGG ACGCTAATTT AGTAGTTATG 
ACATTGCCAA TGCCTCGAAA GGGTGCTGTT TCAGCACCTC TCTATATGGC TTGGCTCGAA 
ACTTTAACGC GTGATATGCC ACCATTTTTA CTTGTTCGAG GAAATCACAC ATCTGTTTTA 
ACTTTTTACT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016922482.1
CDS	836..3970
Translation	MAHENGISGDTDSETVELNPLRRTRFHVNRVDSLEGRASLLGEQETRKSLRHMTREALPRLDNYRNIMSIQAAHRPSLDELHNPTVLNKGAGSHNLPPVQVKPPTSGIKFGWIQGVLLRCLLNIWGVMLFLRLSWVVAQAGIGEAILLILTTTTVTTITSLSMSAISTNGLIKGGGTYYMISRSLGPEFGGSIGLIFSLANAVACSMYVVGFCESMVDCLKSNGVCIVDCDNTDIRIIGCITIVLLLLIVMIGLEWEAKAQIGLLIILLLAIADFMIGTFIGPKDDQERAKGFMGYNADLFKENFYPDYRYSEGMKHNFFSVLAIFFPAATGILAGANISGDLKDPQTAIPKGTLLAILLTTISYLFMALMVGGSVMRDASGDVNDLWNIFNSSYTALSSSNTTNETTESSIIVNSNQIVWSIYSTKFNCTGNCQYGSHNSFQVIELVSAFGPFIYAGCFAATLSSALASLVSAPKVFQALCLDKLYPGIAWFSGDKDKEPIRGYLLTFIIAVGFILIGELNAIAPLISNFFLAAYTLINFSTFHASLAKPIGWRPTFKYYNMWLSLAGAILCVSVMFLISWWTALITLFVVLTLYLIVSYRKPDVNWGSTTQAQTYNNALNAVQQLDRVEEHVKNYRPQLLVLSGTPSARSALIDFAHHITKNNSLFICGHIIETPISYKTRNSMIANCTSWFRANKIKAFYSLVDGANFQDGTTSLLQAAGLGKMKPNILLMGYKQDWATCPRENLNMYFNIMHKALDMHIAVALLRISEGLDCSIGDVEEQKKSIQTIPGNQSFSQLSQASSTSDISIPGSPAPRRSKMINEYPNPTAEDQRENRQNFLPIAKDILNAVTKFQRKQKKGTIDVWWLYDDGGLTLLLPYIISTRRNWSNNKLRVFALANKNSELEYEQRNMASLLSKFRIDYSALKVIPDISKPAQNTTKSFFDSLIANFQETENTKTMNDDIIKDSELIAMKEKTNRHLRLRELLLENSMDANLVVMTLPMPRKGAVSAPLYMAWLETLTRDMPPFLLVRGNHTSVLTFYS

Target ORF information:

RefSeq Version	XM_017066993.1
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana bumetanide-sensitive sodium-(potassium)-chloride cotransporter (LOC108004236), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017066993.1

ORF Insert Sequence:

ATGGCACATG AAAATGGTAT AAGCGGTGAT ACCGATTCCG AAACGGTAGA ATTGAATCCA 
TTGAGAAGAA CCAGATTCCA TGTAAATCGA GTGGACAGTC TTGAAGGTCG AGCGAGCCTT 
CTTGGAGAAC AAGAAACGAG AAAGTCCCTC CGACACATGA CTAGGGAGGC ACTGCCTAGA 
CTGGATAATT ATAGAAACAT TATGAGTATC CAGGCCGCTC ATAGACCGTC TTTGGACGAG 
CTTCACAATC CCACTGTGCT TAACAAGGGT GCAGGATCGC ATAATTTGCC ACCAGTGCAA 
GTGAAGCCAC CGACTTCCGG AATAAAATTC GGATGGATTC AAGGAGTTTT ATTAAGATGC 
CTTCTCAATA TTTGGGGGGT AATGCTTTTT TTACGTCTCT CCTGGGTCGT AGCACAAGCA 
GGCATAGGAG AAGCTATTTT ATTGATTTTA ACAACGACAA CTGTGACTAC GATAACATCG 
TTATCTATGT CGGCAATTAG CACAAATGGT CTCATTAAAG GAGGTGGCAC GTATTACATG 
ATTTCCAGAT CACTGGGGCC AGAATTCGGA GGATCTATAG GACTTATATT TTCTTTGGCG 
AACGCGGTCG CTTGTTCCAT GTATGTTGTT GGATTTTGCG AGAGTATGGT CGATTGCTTA 
AAATCAAATG GAGTTTGTAT TGTTGATTGC GACAATACGG ATATTAGAAT TATAGGTTGT 
ATCACGATAG TTCTGTTACT TTTGATCGTT ATGATTGGAT TGGAATGGGA AGCCAAAGCT 
CAAATTGGTT TGCTGATTAT ATTGCTTTTA GCTATCGCTG ATTTTATGAT CGGCACCTTT 
ATTGGACCTA AAGATGATCA AGAACGGGCT AAAGGATTTA TGGGATATAA TGCTGATCTA 
TTCAAGGAGA ACTTTTATCC GGATTATAGA TATAGTGAGG GAATGAAGCA TAATTTTTTT 
TCAGTCTTGG CTATTTTTTT CCCTGCGGCT ACAGGTATCC TGGCAGGTGC GAATATATCC 
GGTGATTTGA AGGATCCGCA AACAGCTATT CCTAAAGGAA CATTGTTAGC TATTCTTTTA 
ACAACTATAT CTTACCTCTT TATGGCACTT ATGGTGGGCG GCTCAGTTAT GCGAGATGCA 
TCTGGCGATG TTAATGATCT GTGGAACATT TTCAATAGTT CATATACTGC CTTATCAAGT 
TCGAATACGA CTAATGAAAC AACTGAATCT TCAATTATTG TAAATTCCAA TCAAATAGTA 
TGGAGTATAT ATAGTACAAA ATTTAATTGT ACAGGAAATT GTCAATATGG TAGCCATAAT 
AGTTTTCAGG TGATCGAATT GGTTTCTGCA TTTGGACCTT TTATTTATGC TGGATGTTTT 
GCAGCTACTT TATCAAGCGC TTTAGCATCA TTAGTTTCCG CTCCCAAAGT ATTTCAGGCA 
CTTTGTCTTG ATAAATTATA TCCTGGAATA GCATGGTTTA GTGGCGACAA AGATAAAGAA 
CCAATTCGTG GTTATCTTCT TACTTTTATA ATCGCAGTTG GTTTTATTTT AATCGGAGAA 
TTGAATGCTA TAGCTCCTTT GATCTCAAAT TTTTTCTTGG CTGCCTATAC TCTTATTAAT 
TTTAGCACTT TTCATGCTTC GTTGGCAAAA CCAATTGGTT GGAGACCAAC GTTTAAATAT 
TATAATATGT GGCTAAGTCT TGCTGGTGCT ATTCTCTGTG TTTCTGTGAT GTTCTTGATC 
TCATGGTGGA CAGCGTTGAT CACATTATTT GTAGTATTAA CACTTTATTT AATAGTTTCT 
TATAGAAAAC CAGATGTTAA TTGGGGTTCT ACAACACAAG CCCAAACATA TAATAATGCC 
TTAAATGCTG TTCAACAATT AGATAGAGTT GAAGAACATG TTAAAAATTA TAGACCTCAA 
TTGTTAGTAT TAAGTGGCAC TCCAAGTGCA AGATCAGCAC TTATTGATTT TGCACATCAT 
ATTACTAAAA ATAATAGTTT ATTTATTTGT GGCCATATTA TTGAGACACC AATATCATAT 
AAGACGCGAA ATTCCATGAT TGCGAATTGT ACTTCCTGGT TTAGAGCAAA TAAAATTAAG 
GCATTTTATT CTCTGGTGGA TGGTGCAAAT TTTCAAGACG GCACTACTTC TCTTTTACAA 
GCTGCCGGTT TAGGAAAAAT GAAGCCTAAT ATTTTATTAA TGGGCTATAA GCAAGACTGG 
GCAACTTGTC CTCGAGAAAA TTTAAATATG TACTTTAATA TAATGCACAA AGCTTTAGAT 
ATGCATATAG CAGTAGCTCT TCTTCGAATA AGTGAAGGTT TAGATTGTAG TATAGGAGAT 
GTTGAAGAAC AAAAGAAAAG TATCCAAACA ATTCCAGGGA ATCAAAGCTT TTCTCAACTT 
AGCCAAGCCA GTAGTACATC AGATATTTCT ATTCCTGGAA GTCCAGCTCC AAGAAGATCT 
AAAATGATTA ATGAATATCC TAATCCAACT GCAGAAGATC AACGTGAAAA TCGACAAAAT 
TTCTTACCCA TAGCGAAAGA TATACTTAAT GCTGTAACAA AATTTCAACG TAAACAAAAA 
AAGGGTACTA TAGATGTATG GTGGTTGTAT GATGATGGTG GTTTAACTCT TTTATTACCT 
TATATTATCA GTACAAGACG TAATTGGTCT AATAATAAAT TAAGAGTTTT TGCTTTAGCA 
AATAAAAATT CTGAACTCGA ATATGAACAA AGAAATATGG CAAGTCTTTT ATCGAAATTC 
AGGATAGATT ATTCTGCATT GAAAGTTATA CCAGATATTT CAAAACCAGC ACAGAATACT 
ACCAAAAGTT TCTTTGATTC ATTAATAGCA AATTTCCAAG AAACAGAAAA TACAAAAACA 
ATGAATGATG ATATTATCAA AGATTCTGAA TTAATTGCTA TGAAAGAAAA AACAAATAGA 
CATCTACGTT TAAGAGAATT ATTACTTGAA AATTCTATGG ACGCTAATTT AGTAGTTATG 
ACATTGCCAA TGCCTCGAAA GGGTGCTGTT TCAGCACCTC TCTATATGGC TTGGCTCGAA 
ACTTTAACGC GTGATATGCC ACCATTTTTA CTTGTTCGAG GAAATCACAC ATCTGTTTTA 
ACTTTTTACT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108004236 ( XM_017066994.2 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_016922483.1 Apis cerana bumetanide-sensitive sodium-(potassium)-chloride cotransporter (LOC108004236), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAp30532
Clone ID Related Accession (Same CDS sequence)	XM_017066995.1 , XM_017066993.1 , XM_017066994.2 , XM_017066994.1 , XM_017066995.2 , XM_017066993.2
Accession Version	XM_017066994.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-16
Organism	Apis cerana(Asiatic honeybee)
Product	bumetanide-sensitive sodium-(potassium)-chloride cotransporter
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016017734.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 17, 2019 this sequence version replaced XM_017066994.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis cerana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACATG AAAATGGTAT AAGCGGTGAT ACCGATTCCG AAACGGTAGA ATTGAATCCA 
TTGAGAAGAA CCAGATTCCA TGTAAATCGA GTGGACAGTC TTGAAGGTCG AGCGAGCCTT 
CTTGGAGAAC AAGAAACGAG AAAGTCCCTC CGACACATGA CTAGGGAGGC ACTGCCTAGA 
CTGGATAATT ATAGAAACAT TATGAGTATC CAGGCCGCTC ATAGACCGTC TTTGGACGAG 
CTTCACAATC CCACTGTGCT TAACAAGGGT GCAGGATCGC ATAATTTGCC ACCAGTGCAA 
GTGAAGCCAC CGACTTCCGG AATAAAATTC GGATGGATTC AAGGAGTTTT ATTAAGATGC 
CTTCTCAATA TTTGGGGGGT AATGCTTTTT TTACGTCTCT CCTGGGTCGT AGCACAAGCA 
GGCATAGGAG AAGCTATTTT ATTGATTTTA ACAACGACAA CTGTGACTAC GATAACATCG 
TTATCTATGT CGGCAATTAG CACAAATGGT CTCATTAAAG GAGGTGGCAC GTATTACATG 
ATTTCCAGAT CACTGGGGCC AGAATTCGGA GGATCTATAG GACTTATATT TTCTTTGGCG 
AACGCGGTCG CTTGTTCCAT GTATGTTGTT GGATTTTGCG AGAGTATGGT CGATTGCTTA 
AAATCAAATG GAGTTTGTAT TGTTGATTGC GACAATACGG ATATTAGAAT TATAGGTTGT 
ATCACGATAG TTCTGTTACT TTTGATCGTT ATGATTGGAT TGGAATGGGA AGCCAAAGCT 
CAAATTGGTT TGCTGATTAT ATTGCTTTTA GCTATCGCTG ATTTTATGAT CGGCACCTTT 
ATTGGACCTA AAGATGATCA AGAACGGGCT AAAGGATTTA TGGGATATAA TGCTGATCTA 
TTCAAGGAGA ACTTTTATCC GGATTATAGA TATAGTGAGG GAATGAAGCA TAATTTTTTT 
TCAGTCTTGG CTATTTTTTT CCCTGCGGCT ACAGGTATCC TGGCAGGTGC GAATATATCC 
GGTGATTTGA AGGATCCGCA AACAGCTATT CCTAAAGGAA CATTGTTAGC TATTCTTTTA 
ACAACTATAT CTTACCTCTT TATGGCACTT ATGGTGGGCG GCTCAGTTAT GCGAGATGCA 
TCTGGCGATG TTAATGATCT GTGGAACATT TTCAATAGTT CATATACTGC CTTATCAAGT 
TCGAATACGA CTAATGAAAC AACTGAATCT TCAATTATTG TAAATTCCAA TCAAATAGTA 
TGGAGTATAT ATAGTACAAA ATTTAATTGT ACAGGAAATT GTCAATATGG TAGCCATAAT 
AGTTTTCAGG TGATCGAATT GGTTTCTGCA TTTGGACCTT TTATTTATGC TGGATGTTTT 
GCAGCTACTT TATCAAGCGC TTTAGCATCA TTAGTTTCCG CTCCCAAAGT ATTTCAGGCA 
CTTTGTCTTG ATAAATTATA TCCTGGAATA GCATGGTTTA GTGGCGACAA AGATAAAGAA 
CCAATTCGTG GTTATCTTCT TACTTTTATA ATCGCAGTTG GTTTTATTTT AATCGGAGAA 
TTGAATGCTA TAGCTCCTTT GATCTCAAAT TTTTTCTTGG CTGCCTATAC TCTTATTAAT 
TTTAGCACTT TTCATGCTTC GTTGGCAAAA CCAATTGGTT GGAGACCAAC GTTTAAATAT 
TATAATATGT GGCTAAGTCT TGCTGGTGCT ATTCTCTGTG TTTCTGTGAT GTTCTTGATC 
TCATGGTGGA CAGCGTTGAT CACATTATTT GTAGTATTAA CACTTTATTT AATAGTTTCT 
TATAGAAAAC CAGATGTTAA TTGGGGTTCT ACAACACAAG CCCAAACATA TAATAATGCC 
TTAAATGCTG TTCAACAATT AGATAGAGTT GAAGAACATG TTAAAAATTA TAGACCTCAA 
TTGTTAGTAT TAAGTGGCAC TCCAAGTGCA AGATCAGCAC TTATTGATTT TGCACATCAT 
ATTACTAAAA ATAATAGTTT ATTTATTTGT GGCCATATTA TTGAGACACC AATATCATAT 
AAGACGCGAA ATTCCATGAT TGCGAATTGT ACTTCCTGGT TTAGAGCAAA TAAAATTAAG 
GCATTTTATT CTCTGGTGGA TGGTGCAAAT TTTCAAGACG GCACTACTTC TCTTTTACAA 
GCTGCCGGTT TAGGAAAAAT GAAGCCTAAT ATTTTATTAA TGGGCTATAA GCAAGACTGG 
GCAACTTGTC CTCGAGAAAA TTTAAATATG TACTTTAATA TAATGCACAA AGCTTTAGAT 
ATGCATATAG CAGTAGCTCT TCTTCGAATA AGTGAAGGTT TAGATTGTAG TATAGGAGAT 
GTTGAAGAAC AAAAGAAAAG TATCCAAACA ATTCCAGGGA ATCAAAGCTT TTCTCAACTT 
AGCCAAGCCA GTAGTACATC AGATATTTCT ATTCCTGGAA GTCCAGCTCC AAGAAGATCT 
AAAATGATTA ATGAATATCC TAATCCAACT GCAGAAGATC AACGTGAAAA TCGACAAAAT 
TTCTTACCCA TAGCGAAAGA TATACTTAAT GCTGTAACAA AATTTCAACG TAAACAAAAA 
AAGGGTACTA TAGATGTATG GTGGTTGTAT GATGATGGTG GTTTAACTCT TTTATTACCT 
TATATTATCA GTACAAGACG TAATTGGTCT AATAATAAAT TAAGAGTTTT TGCTTTAGCA 
AATAAAAATT CTGAACTCGA ATATGAACAA AGAAATATGG CAAGTCTTTT ATCGAAATTC 
AGGATAGATT ATTCTGCATT GAAAGTTATA CCAGATATTT CAAAACCAGC ACAGAATACT 
ACCAAAAGTT TCTTTGATTC ATTAATAGCA AATTTCCAAG AAACAGAAAA TACAAAAACA 
ATGAATGATG ATATTATCAA AGATTCTGAA TTAATTGCTA TGAAAGAAAA AACAAATAGA 
CATCTACGTT TAAGAGAATT ATTACTTGAA AATTCTATGG ACGCTAATTT AGTAGTTATG 
ACATTGCCAA TGCCTCGAAA GGGTGCTGTT TCAGCACCTC TCTATATGGC TTGGCTCGAA 
ACTTTAACGC GTGATATGCC ACCATTTTTA CTTGTTCGAG GAAATCACAC ATCTGTTTTA 
ACTTTTTACT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016922483.1
CDS	694..3828
Translation	MAHENGISGDTDSETVELNPLRRTRFHVNRVDSLEGRASLLGEQETRKSLRHMTREALPRLDNYRNIMSIQAAHRPSLDELHNPTVLNKGAGSHNLPPVQVKPPTSGIKFGWIQGVLLRCLLNIWGVMLFLRLSWVVAQAGIGEAILLILTTTTVTTITSLSMSAISTNGLIKGGGTYYMISRSLGPEFGGSIGLIFSLANAVACSMYVVGFCESMVDCLKSNGVCIVDCDNTDIRIIGCITIVLLLLIVMIGLEWEAKAQIGLLIILLLAIADFMIGTFIGPKDDQERAKGFMGYNADLFKENFYPDYRYSEGMKHNFFSVLAIFFPAATGILAGANISGDLKDPQTAIPKGTLLAILLTTISYLFMALMVGGSVMRDASGDVNDLWNIFNSSYTALSSSNTTNETTESSIIVNSNQIVWSIYSTKFNCTGNCQYGSHNSFQVIELVSAFGPFIYAGCFAATLSSALASLVSAPKVFQALCLDKLYPGIAWFSGDKDKEPIRGYLLTFIIAVGFILIGELNAIAPLISNFFLAAYTLINFSTFHASLAKPIGWRPTFKYYNMWLSLAGAILCVSVMFLISWWTALITLFVVLTLYLIVSYRKPDVNWGSTTQAQTYNNALNAVQQLDRVEEHVKNYRPQLLVLSGTPSARSALIDFAHHITKNNSLFICGHIIETPISYKTRNSMIANCTSWFRANKIKAFYSLVDGANFQDGTTSLLQAAGLGKMKPNILLMGYKQDWATCPRENLNMYFNIMHKALDMHIAVALLRISEGLDCSIGDVEEQKKSIQTIPGNQSFSQLSQASSTSDISIPGSPAPRRSKMINEYPNPTAEDQRENRQNFLPIAKDILNAVTKFQRKQKKGTIDVWWLYDDGGLTLLLPYIISTRRNWSNNKLRVFALANKNSELEYEQRNMASLLSKFRIDYSALKVIPDISKPAQNTTKSFFDSLIANFQETENTKTMNDDIIKDSELIAMKEKTNRHLRLRELLLENSMDANLVVMTLPMPRKGAVSAPLYMAWLETLTRDMPPFLLVRGNHTSVLTFYS

Target ORF information:

RefSeq Version	XM_017066994.2
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana bumetanide-sensitive sodium-(potassium)-chloride cotransporter (LOC108004236), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017066994.2

ORF Insert Sequence:

ATGGCACATG AAAATGGTAT AAGCGGTGAT ACCGATTCCG AAACGGTAGA ATTGAATCCA 
TTGAGAAGAA CCAGATTCCA TGTAAATCGA GTGGACAGTC TTGAAGGTCG AGCGAGCCTT 
CTTGGAGAAC AAGAAACGAG AAAGTCCCTC CGACACATGA CTAGGGAGGC ACTGCCTAGA 
CTGGATAATT ATAGAAACAT TATGAGTATC CAGGCCGCTC ATAGACCGTC TTTGGACGAG 
CTTCACAATC CCACTGTGCT TAACAAGGGT GCAGGATCGC ATAATTTGCC ACCAGTGCAA 
GTGAAGCCAC CGACTTCCGG AATAAAATTC GGATGGATTC AAGGAGTTTT ATTAAGATGC 
CTTCTCAATA TTTGGGGGGT AATGCTTTTT TTACGTCTCT CCTGGGTCGT AGCACAAGCA 
GGCATAGGAG AAGCTATTTT ATTGATTTTA ACAACGACAA CTGTGACTAC GATAACATCG 
TTATCTATGT CGGCAATTAG CACAAATGGT CTCATTAAAG GAGGTGGCAC GTATTACATG 
ATTTCCAGAT CACTGGGGCC AGAATTCGGA GGATCTATAG GACTTATATT TTCTTTGGCG 
AACGCGGTCG CTTGTTCCAT GTATGTTGTT GGATTTTGCG AGAGTATGGT CGATTGCTTA 
AAATCAAATG GAGTTTGTAT TGTTGATTGC GACAATACGG ATATTAGAAT TATAGGTTGT 
ATCACGATAG TTCTGTTACT TTTGATCGTT ATGATTGGAT TGGAATGGGA AGCCAAAGCT 
CAAATTGGTT TGCTGATTAT ATTGCTTTTA GCTATCGCTG ATTTTATGAT CGGCACCTTT 
ATTGGACCTA AAGATGATCA AGAACGGGCT AAAGGATTTA TGGGATATAA TGCTGATCTA 
TTCAAGGAGA ACTTTTATCC GGATTATAGA TATAGTGAGG GAATGAAGCA TAATTTTTTT 
TCAGTCTTGG CTATTTTTTT CCCTGCGGCT ACAGGTATCC TGGCAGGTGC GAATATATCC 
GGTGATTTGA AGGATCCGCA AACAGCTATT CCTAAAGGAA CATTGTTAGC TATTCTTTTA 
ACAACTATAT CTTACCTCTT TATGGCACTT ATGGTGGGCG GCTCAGTTAT GCGAGATGCA 
TCTGGCGATG TTAATGATCT GTGGAACATT TTCAATAGTT CATATACTGC CTTATCAAGT 
TCGAATACGA CTAATGAAAC AACTGAATCT TCAATTATTG TAAATTCCAA TCAAATAGTA 
TGGAGTATAT ATAGTACAAA ATTTAATTGT ACAGGAAATT GTCAATATGG TAGCCATAAT 
AGTTTTCAGG TGATCGAATT GGTTTCTGCA TTTGGACCTT TTATTTATGC TGGATGTTTT 
GCAGCTACTT TATCAAGCGC TTTAGCATCA TTAGTTTCCG CTCCCAAAGT ATTTCAGGCA 
CTTTGTCTTG ATAAATTATA TCCTGGAATA GCATGGTTTA GTGGCGACAA AGATAAAGAA 
CCAATTCGTG GTTATCTTCT TACTTTTATA ATCGCAGTTG GTTTTATTTT AATCGGAGAA 
TTGAATGCTA TAGCTCCTTT GATCTCAAAT TTTTTCTTGG CTGCCTATAC TCTTATTAAT 
TTTAGCACTT TTCATGCTTC GTTGGCAAAA CCAATTGGTT GGAGACCAAC GTTTAAATAT 
TATAATATGT GGCTAAGTCT TGCTGGTGCT ATTCTCTGTG TTTCTGTGAT GTTCTTGATC 
TCATGGTGGA CAGCGTTGAT CACATTATTT GTAGTATTAA CACTTTATTT AATAGTTTCT 
TATAGAAAAC CAGATGTTAA TTGGGGTTCT ACAACACAAG CCCAAACATA TAATAATGCC 
TTAAATGCTG TTCAACAATT AGATAGAGTT GAAGAACATG TTAAAAATTA TAGACCTCAA 
TTGTTAGTAT TAAGTGGCAC TCCAAGTGCA AGATCAGCAC TTATTGATTT TGCACATCAT 
ATTACTAAAA ATAATAGTTT ATTTATTTGT GGCCATATTA TTGAGACACC AATATCATAT 
AAGACGCGAA ATTCCATGAT TGCGAATTGT ACTTCCTGGT TTAGAGCAAA TAAAATTAAG 
GCATTTTATT CTCTGGTGGA TGGTGCAAAT TTTCAAGACG GCACTACTTC TCTTTTACAA 
GCTGCCGGTT TAGGAAAAAT GAAGCCTAAT ATTTTATTAA TGGGCTATAA GCAAGACTGG 
GCAACTTGTC CTCGAGAAAA TTTAAATATG TACTTTAATA TAATGCACAA AGCTTTAGAT 
ATGCATATAG CAGTAGCTCT TCTTCGAATA AGTGAAGGTT TAGATTGTAG TATAGGAGAT 
GTTGAAGAAC AAAAGAAAAG TATCCAAACA ATTCCAGGGA ATCAAAGCTT TTCTCAACTT 
AGCCAAGCCA GTAGTACATC AGATATTTCT ATTCCTGGAA GTCCAGCTCC AAGAAGATCT 
AAAATGATTA ATGAATATCC TAATCCAACT GCAGAAGATC AACGTGAAAA TCGACAAAAT 
TTCTTACCCA TAGCGAAAGA TATACTTAAT GCTGTAACAA AATTTCAACG TAAACAAAAA 
AAGGGTACTA TAGATGTATG GTGGTTGTAT GATGATGGTG GTTTAACTCT TTTATTACCT 
TATATTATCA GTACAAGACG TAATTGGTCT AATAATAAAT TAAGAGTTTT TGCTTTAGCA 
AATAAAAATT CTGAACTCGA ATATGAACAA AGAAATATGG CAAGTCTTTT ATCGAAATTC 
AGGATAGATT ATTCTGCATT GAAAGTTATA CCAGATATTT CAAAACCAGC ACAGAATACT 
ACCAAAAGTT TCTTTGATTC ATTAATAGCA AATTTCCAAG AAACAGAAAA TACAAAAACA 
ATGAATGATG ATATTATCAA AGATTCTGAA TTAATTGCTA TGAAAGAAAA AACAAATAGA 
CATCTACGTT TAAGAGAATT ATTACTTGAA AATTCTATGG ACGCTAATTT AGTAGTTATG 
ACATTGCCAA TGCCTCGAAA GGGTGCTGTT TCAGCACCTC TCTATATGGC TTGGCTCGAA 
ACTTTAACGC GTGATATGCC ACCATTTTTA CTTGTTCGAG GAAATCACAC ATCTGTTTTA 
ACTTTTTACT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108004236 ( XM_017066994.1 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_016922483.1 Apis cerana bumetanide-sensitive sodium-(potassium)-chloride cotransporter (LOC108004236), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAp30532
Clone ID Related Accession (Same CDS sequence)	XM_017066995.1 , XM_017066993.1 , XM_017066994.2 , XM_017066994.1 , XM_017066995.2 , XM_017066993.2
Accession Version	XM_017066994.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-08
Organism	Apis cerana(Asiatic honeybee)
Product	bumetanide-sensitive sodium-(potassium)-chloride cotransporter
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016017734.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis cerana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACATG AAAATGGTAT AAGCGGTGAT ACCGATTCCG AAACGGTAGA ATTGAATCCA 
TTGAGAAGAA CCAGATTCCA TGTAAATCGA GTGGACAGTC TTGAAGGTCG AGCGAGCCTT 
CTTGGAGAAC AAGAAACGAG AAAGTCCCTC CGACACATGA CTAGGGAGGC ACTGCCTAGA 
CTGGATAATT ATAGAAACAT TATGAGTATC CAGGCCGCTC ATAGACCGTC TTTGGACGAG 
CTTCACAATC CCACTGTGCT TAACAAGGGT GCAGGATCGC ATAATTTGCC ACCAGTGCAA 
GTGAAGCCAC CGACTTCCGG AATAAAATTC GGATGGATTC AAGGAGTTTT ATTAAGATGC 
CTTCTCAATA TTTGGGGGGT AATGCTTTTT TTACGTCTCT CCTGGGTCGT AGCACAAGCA 
GGCATAGGAG AAGCTATTTT ATTGATTTTA ACAACGACAA CTGTGACTAC GATAACATCG 
TTATCTATGT CGGCAATTAG CACAAATGGT CTCATTAAAG GAGGTGGCAC GTATTACATG 
ATTTCCAGAT CACTGGGGCC AGAATTCGGA GGATCTATAG GACTTATATT TTCTTTGGCG 
AACGCGGTCG CTTGTTCCAT GTATGTTGTT GGATTTTGCG AGAGTATGGT CGATTGCTTA 
AAATCAAATG GAGTTTGTAT TGTTGATTGC GACAATACGG ATATTAGAAT TATAGGTTGT 
ATCACGATAG TTCTGTTACT TTTGATCGTT ATGATTGGAT TGGAATGGGA AGCCAAAGCT 
CAAATTGGTT TGCTGATTAT ATTGCTTTTA GCTATCGCTG ATTTTATGAT CGGCACCTTT 
ATTGGACCTA AAGATGATCA AGAACGGGCT AAAGGATTTA TGGGATATAA TGCTGATCTA 
TTCAAGGAGA ACTTTTATCC GGATTATAGA TATAGTGAGG GAATGAAGCA TAATTTTTTT 
TCAGTCTTGG CTATTTTTTT CCCTGCGGCT ACAGGTATCC TGGCAGGTGC GAATATATCC 
GGTGATTTGA AGGATCCGCA AACAGCTATT CCTAAAGGAA CATTGTTAGC TATTCTTTTA 
ACAACTATAT CTTACCTCTT TATGGCACTT ATGGTGGGCG GCTCAGTTAT GCGAGATGCA 
TCTGGCGATG TTAATGATCT GTGGAACATT TTCAATAGTT CATATACTGC CTTATCAAGT 
TCGAATACGA CTAATGAAAC AACTGAATCT TCAATTATTG TAAATTCCAA TCAAATAGTA 
TGGAGTATAT ATAGTACAAA ATTTAATTGT ACAGGAAATT GTCAATATGG TAGCCATAAT 
AGTTTTCAGG TGATCGAATT GGTTTCTGCA TTTGGACCTT TTATTTATGC TGGATGTTTT 
GCAGCTACTT TATCAAGCGC TTTAGCATCA TTAGTTTCCG CTCCCAAAGT ATTTCAGGCA 
CTTTGTCTTG ATAAATTATA TCCTGGAATA GCATGGTTTA GTGGCGACAA AGATAAAGAA 
CCAATTCGTG GTTATCTTCT TACTTTTATA ATCGCAGTTG GTTTTATTTT AATCGGAGAA 
TTGAATGCTA TAGCTCCTTT GATCTCAAAT TTTTTCTTGG CTGCCTATAC TCTTATTAAT 
TTTAGCACTT TTCATGCTTC GTTGGCAAAA CCAATTGGTT GGAGACCAAC GTTTAAATAT 
TATAATATGT GGCTAAGTCT TGCTGGTGCT ATTCTCTGTG TTTCTGTGAT GTTCTTGATC 
TCATGGTGGA CAGCGTTGAT CACATTATTT GTAGTATTAA CACTTTATTT AATAGTTTCT 
TATAGAAAAC CAGATGTTAA TTGGGGTTCT ACAACACAAG CCCAAACATA TAATAATGCC 
TTAAATGCTG TTCAACAATT AGATAGAGTT GAAGAACATG TTAAAAATTA TAGACCTCAA 
TTGTTAGTAT TAAGTGGCAC TCCAAGTGCA AGATCAGCAC TTATTGATTT TGCACATCAT 
ATTACTAAAA ATAATAGTTT ATTTATTTGT GGCCATATTA TTGAGACACC AATATCATAT 
AAGACGCGAA ATTCCATGAT TGCGAATTGT ACTTCCTGGT TTAGAGCAAA TAAAATTAAG 
GCATTTTATT CTCTGGTGGA TGGTGCAAAT TTTCAAGACG GCACTACTTC TCTTTTACAA 
GCTGCCGGTT TAGGAAAAAT GAAGCCTAAT ATTTTATTAA TGGGCTATAA GCAAGACTGG 
GCAACTTGTC CTCGAGAAAA TTTAAATATG TACTTTAATA TAATGCACAA AGCTTTAGAT 
ATGCATATAG CAGTAGCTCT TCTTCGAATA AGTGAAGGTT TAGATTGTAG TATAGGAGAT 
GTTGAAGAAC AAAAGAAAAG TATCCAAACA ATTCCAGGGA ATCAAAGCTT TTCTCAACTT 
AGCCAAGCCA GTAGTACATC AGATATTTCT ATTCCTGGAA GTCCAGCTCC AAGAAGATCT 
AAAATGATTA ATGAATATCC TAATCCAACT GCAGAAGATC AACGTGAAAA TCGACAAAAT 
TTCTTACCCA TAGCGAAAGA TATACTTAAT GCTGTAACAA AATTTCAACG TAAACAAAAA 
AAGGGTACTA TAGATGTATG GTGGTTGTAT GATGATGGTG GTTTAACTCT TTTATTACCT 
TATATTATCA GTACAAGACG TAATTGGTCT AATAATAAAT TAAGAGTTTT TGCTTTAGCA 
AATAAAAATT CTGAACTCGA ATATGAACAA AGAAATATGG CAAGTCTTTT ATCGAAATTC 
AGGATAGATT ATTCTGCATT GAAAGTTATA CCAGATATTT CAAAACCAGC ACAGAATACT 
ACCAAAAGTT TCTTTGATTC ATTAATAGCA AATTTCCAAG AAACAGAAAA TACAAAAACA 
ATGAATGATG ATATTATCAA AGATTCTGAA TTAATTGCTA TGAAAGAAAA AACAAATAGA 
CATCTACGTT TAAGAGAATT ATTACTTGAA AATTCTATGG ACGCTAATTT AGTAGTTATG 
ACATTGCCAA TGCCTCGAAA GGGTGCTGTT TCAGCACCTC TCTATATGGC TTGGCTCGAA 
ACTTTAACGC GTGATATGCC ACCATTTTTA CTTGTTCGAG GAAATCACAC ATCTGTTTTA 
ACTTTTTACT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016922483.1
CDS	674..3808
Translation	MAHENGISGDTDSETVELNPLRRTRFHVNRVDSLEGRASLLGEQETRKSLRHMTREALPRLDNYRNIMSIQAAHRPSLDELHNPTVLNKGAGSHNLPPVQVKPPTSGIKFGWIQGVLLRCLLNIWGVMLFLRLSWVVAQAGIGEAILLILTTTTVTTITSLSMSAISTNGLIKGGGTYYMISRSLGPEFGGSIGLIFSLANAVACSMYVVGFCESMVDCLKSNGVCIVDCDNTDIRIIGCITIVLLLLIVMIGLEWEAKAQIGLLIILLLAIADFMIGTFIGPKDDQERAKGFMGYNADLFKENFYPDYRYSEGMKHNFFSVLAIFFPAATGILAGANISGDLKDPQTAIPKGTLLAILLTTISYLFMALMVGGSVMRDASGDVNDLWNIFNSSYTALSSSNTTNETTESSIIVNSNQIVWSIYSTKFNCTGNCQYGSHNSFQVIELVSAFGPFIYAGCFAATLSSALASLVSAPKVFQALCLDKLYPGIAWFSGDKDKEPIRGYLLTFIIAVGFILIGELNAIAPLISNFFLAAYTLINFSTFHASLAKPIGWRPTFKYYNMWLSLAGAILCVSVMFLISWWTALITLFVVLTLYLIVSYRKPDVNWGSTTQAQTYNNALNAVQQLDRVEEHVKNYRPQLLVLSGTPSARSALIDFAHHITKNNSLFICGHIIETPISYKTRNSMIANCTSWFRANKIKAFYSLVDGANFQDGTTSLLQAAGLGKMKPNILLMGYKQDWATCPRENLNMYFNIMHKALDMHIAVALLRISEGLDCSIGDVEEQKKSIQTIPGNQSFSQLSQASSTSDISIPGSPAPRRSKMINEYPNPTAEDQRENRQNFLPIAKDILNAVTKFQRKQKKGTIDVWWLYDDGGLTLLLPYIISTRRNWSNNKLRVFALANKNSELEYEQRNMASLLSKFRIDYSALKVIPDISKPAQNTTKSFFDSLIANFQETENTKTMNDDIIKDSELIAMKEKTNRHLRLRELLLENSMDANLVVMTLPMPRKGAVSAPLYMAWLETLTRDMPPFLLVRGNHTSVLTFYS

Target ORF information:

RefSeq Version	XM_017066994.1
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana bumetanide-sensitive sodium-(potassium)-chloride cotransporter (LOC108004236), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017066994.1

ORF Insert Sequence:

ATGGCACATG AAAATGGTAT AAGCGGTGAT ACCGATTCCG AAACGGTAGA ATTGAATCCA 
TTGAGAAGAA CCAGATTCCA TGTAAATCGA GTGGACAGTC TTGAAGGTCG AGCGAGCCTT 
CTTGGAGAAC AAGAAACGAG AAAGTCCCTC CGACACATGA CTAGGGAGGC ACTGCCTAGA 
CTGGATAATT ATAGAAACAT TATGAGTATC CAGGCCGCTC ATAGACCGTC TTTGGACGAG 
CTTCACAATC CCACTGTGCT TAACAAGGGT GCAGGATCGC ATAATTTGCC ACCAGTGCAA 
GTGAAGCCAC CGACTTCCGG AATAAAATTC GGATGGATTC AAGGAGTTTT ATTAAGATGC 
CTTCTCAATA TTTGGGGGGT AATGCTTTTT TTACGTCTCT CCTGGGTCGT AGCACAAGCA 
GGCATAGGAG AAGCTATTTT ATTGATTTTA ACAACGACAA CTGTGACTAC GATAACATCG 
TTATCTATGT CGGCAATTAG CACAAATGGT CTCATTAAAG GAGGTGGCAC GTATTACATG 
ATTTCCAGAT CACTGGGGCC AGAATTCGGA GGATCTATAG GACTTATATT TTCTTTGGCG 
AACGCGGTCG CTTGTTCCAT GTATGTTGTT GGATTTTGCG AGAGTATGGT CGATTGCTTA 
AAATCAAATG GAGTTTGTAT TGTTGATTGC GACAATACGG ATATTAGAAT TATAGGTTGT 
ATCACGATAG TTCTGTTACT TTTGATCGTT ATGATTGGAT TGGAATGGGA AGCCAAAGCT 
CAAATTGGTT TGCTGATTAT ATTGCTTTTA GCTATCGCTG ATTTTATGAT CGGCACCTTT 
ATTGGACCTA AAGATGATCA AGAACGGGCT AAAGGATTTA TGGGATATAA TGCTGATCTA 
TTCAAGGAGA ACTTTTATCC GGATTATAGA TATAGTGAGG GAATGAAGCA TAATTTTTTT 
TCAGTCTTGG CTATTTTTTT CCCTGCGGCT ACAGGTATCC TGGCAGGTGC GAATATATCC 
GGTGATTTGA AGGATCCGCA AACAGCTATT CCTAAAGGAA CATTGTTAGC TATTCTTTTA 
ACAACTATAT CTTACCTCTT TATGGCACTT ATGGTGGGCG GCTCAGTTAT GCGAGATGCA 
TCTGGCGATG TTAATGATCT GTGGAACATT TTCAATAGTT CATATACTGC CTTATCAAGT 
TCGAATACGA CTAATGAAAC AACTGAATCT TCAATTATTG TAAATTCCAA TCAAATAGTA 
TGGAGTATAT ATAGTACAAA ATTTAATTGT ACAGGAAATT GTCAATATGG TAGCCATAAT 
AGTTTTCAGG TGATCGAATT GGTTTCTGCA TTTGGACCTT TTATTTATGC TGGATGTTTT 
GCAGCTACTT TATCAAGCGC TTTAGCATCA TTAGTTTCCG CTCCCAAAGT ATTTCAGGCA 
CTTTGTCTTG ATAAATTATA TCCTGGAATA GCATGGTTTA GTGGCGACAA AGATAAAGAA 
CCAATTCGTG GTTATCTTCT TACTTTTATA ATCGCAGTTG GTTTTATTTT AATCGGAGAA 
TTGAATGCTA TAGCTCCTTT GATCTCAAAT TTTTTCTTGG CTGCCTATAC TCTTATTAAT 
TTTAGCACTT TTCATGCTTC GTTGGCAAAA CCAATTGGTT GGAGACCAAC GTTTAAATAT 
TATAATATGT GGCTAAGTCT TGCTGGTGCT ATTCTCTGTG TTTCTGTGAT GTTCTTGATC 
TCATGGTGGA CAGCGTTGAT CACATTATTT GTAGTATTAA CACTTTATTT AATAGTTTCT 
TATAGAAAAC CAGATGTTAA TTGGGGTTCT ACAACACAAG CCCAAACATA TAATAATGCC 
TTAAATGCTG TTCAACAATT AGATAGAGTT GAAGAACATG TTAAAAATTA TAGACCTCAA 
TTGTTAGTAT TAAGTGGCAC TCCAAGTGCA AGATCAGCAC TTATTGATTT TGCACATCAT 
ATTACTAAAA ATAATAGTTT ATTTATTTGT GGCCATATTA TTGAGACACC AATATCATAT 
AAGACGCGAA ATTCCATGAT TGCGAATTGT ACTTCCTGGT TTAGAGCAAA TAAAATTAAG 
GCATTTTATT CTCTGGTGGA TGGTGCAAAT TTTCAAGACG GCACTACTTC TCTTTTACAA 
GCTGCCGGTT TAGGAAAAAT GAAGCCTAAT ATTTTATTAA TGGGCTATAA GCAAGACTGG 
GCAACTTGTC CTCGAGAAAA TTTAAATATG TACTTTAATA TAATGCACAA AGCTTTAGAT 
ATGCATATAG CAGTAGCTCT TCTTCGAATA AGTGAAGGTT TAGATTGTAG TATAGGAGAT 
GTTGAAGAAC AAAAGAAAAG TATCCAAACA ATTCCAGGGA ATCAAAGCTT TTCTCAACTT 
AGCCAAGCCA GTAGTACATC AGATATTTCT ATTCCTGGAA GTCCAGCTCC AAGAAGATCT 
AAAATGATTA ATGAATATCC TAATCCAACT GCAGAAGATC AACGTGAAAA TCGACAAAAT 
TTCTTACCCA TAGCGAAAGA TATACTTAAT GCTGTAACAA AATTTCAACG TAAACAAAAA 
AAGGGTACTA TAGATGTATG GTGGTTGTAT GATGATGGTG GTTTAACTCT TTTATTACCT 
TATATTATCA GTACAAGACG TAATTGGTCT AATAATAAAT TAAGAGTTTT TGCTTTAGCA 
AATAAAAATT CTGAACTCGA ATATGAACAA AGAAATATGG CAAGTCTTTT ATCGAAATTC 
AGGATAGATT ATTCTGCATT GAAAGTTATA CCAGATATTT CAAAACCAGC ACAGAATACT 
ACCAAAAGTT TCTTTGATTC ATTAATAGCA AATTTCCAAG AAACAGAAAA TACAAAAACA 
ATGAATGATG ATATTATCAA AGATTCTGAA TTAATTGCTA TGAAAGAAAA AACAAATAGA 
CATCTACGTT TAAGAGAATT ATTACTTGAA AATTCTATGG ACGCTAATTT AGTAGTTATG 
ACATTGCCAA TGCCTCGAAA GGGTGCTGTT TCAGCACCTC TCTATATGGC TTGGCTCGAA 
ACTTTAACGC GTGATATGCC ACCATTTTTA CTTGTTCGAG GAAATCACAC ATCTGTTTTA 
ACTTTTTACT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108004236 ( XM_017066995.2 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_016922484.1 Apis cerana bumetanide-sensitive sodium-(potassium)-chloride cotransporter (LOC108004236), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAp30532
Clone ID Related Accession (Same CDS sequence)	XM_017066995.1 , XM_017066993.1 , XM_017066994.2 , XM_017066994.1 , XM_017066995.2 , XM_017066993.2
Accession Version	XM_017066995.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-16
Organism	Apis cerana(Asiatic honeybee)
Product	bumetanide-sensitive sodium-(potassium)-chloride cotransporter
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016017734.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 17, 2019 this sequence version replaced XM_017066995.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis cerana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACATG AAAATGGTAT AAGCGGTGAT ACCGATTCCG AAACGGTAGA ATTGAATCCA 
TTGAGAAGAA CCAGATTCCA TGTAAATCGA GTGGACAGTC TTGAAGGTCG AGCGAGCCTT 
CTTGGAGAAC AAGAAACGAG AAAGTCCCTC CGACACATGA CTAGGGAGGC ACTGCCTAGA 
CTGGATAATT ATAGAAACAT TATGAGTATC CAGGCCGCTC ATAGACCGTC TTTGGACGAG 
CTTCACAATC CCACTGTGCT TAACAAGGGT GCAGGATCGC ATAATTTGCC ACCAGTGCAA 
GTGAAGCCAC CGACTTCCGG AATAAAATTC GGATGGATTC AAGGAGTTTT ATTAAGATGC 
CTTCTCAATA TTTGGGGGGT AATGCTTTTT TTACGTCTCT CCTGGGTCGT AGCACAAGCA 
GGCATAGGAG AAGCTATTTT ATTGATTTTA ACAACGACAA CTGTGACTAC GATAACATCG 
TTATCTATGT CGGCAATTAG CACAAATGGT CTCATTAAAG GAGGTGGCAC GTATTACATG 
ATTTCCAGAT CACTGGGGCC AGAATTCGGA GGATCTATAG GACTTATATT TTCTTTGGCG 
AACGCGGTCG CTTGTTCCAT GTATGTTGTT GGATTTTGCG AGAGTATGGT CGATTGCTTA 
AAATCAAATG GAGTTTGTAT TGTTGATTGC GACAATACGG ATATTAGAAT TATAGGTTGT 
ATCACGATAG TTCTGTTACT TTTGATCGTT ATGATTGGAT TGGAATGGGA AGCCAAAGCT 
CAAATTGGTT TGCTGATTAT ATTGCTTTTA GCTATCGCTG ATTTTATGAT CGGCACCTTT 
ATTGGACCTA AAGATGATCA AGAACGGGCT AAAGGATTTA TGGGATATAA TGCTGATCTA 
TTCAAGGAGA ACTTTTATCC GGATTATAGA TATAGTGAGG GAATGAAGCA TAATTTTTTT 
TCAGTCTTGG CTATTTTTTT CCCTGCGGCT ACAGGTATCC TGGCAGGTGC GAATATATCC 
GGTGATTTGA AGGATCCGCA AACAGCTATT CCTAAAGGAA CATTGTTAGC TATTCTTTTA 
ACAACTATAT CTTACCTCTT TATGGCACTT ATGGTGGGCG GCTCAGTTAT GCGAGATGCA 
TCTGGCGATG TTAATGATCT GTGGAACATT TTCAATAGTT CATATACTGC CTTATCAAGT 
TCGAATACGA CTAATGAAAC AACTGAATCT TCAATTATTG TAAATTCCAA TCAAATAGTA 
TGGAGTATAT ATAGTACAAA ATTTAATTGT ACAGGAAATT GTCAATATGG TAGCCATAAT 
AGTTTTCAGG TGATCGAATT GGTTTCTGCA TTTGGACCTT TTATTTATGC TGGATGTTTT 
GCAGCTACTT TATCAAGCGC TTTAGCATCA TTAGTTTCCG CTCCCAAAGT ATTTCAGGCA 
CTTTGTCTTG ATAAATTATA TCCTGGAATA GCATGGTTTA GTGGCGACAA AGATAAAGAA 
CCAATTCGTG GTTATCTTCT TACTTTTATA ATCGCAGTTG GTTTTATTTT AATCGGAGAA 
TTGAATGCTA TAGCTCCTTT GATCTCAAAT TTTTTCTTGG CTGCCTATAC TCTTATTAAT 
TTTAGCACTT TTCATGCTTC GTTGGCAAAA CCAATTGGTT GGAGACCAAC GTTTAAATAT 
TATAATATGT GGCTAAGTCT TGCTGGTGCT ATTCTCTGTG TTTCTGTGAT GTTCTTGATC 
TCATGGTGGA CAGCGTTGAT CACATTATTT GTAGTATTAA CACTTTATTT AATAGTTTCT 
TATAGAAAAC CAGATGTTAA TTGGGGTTCT ACAACACAAG CCCAAACATA TAATAATGCC 
TTAAATGCTG TTCAACAATT AGATAGAGTT GAAGAACATG TTAAAAATTA TAGACCTCAA 
TTGTTAGTAT TAAGTGGCAC TCCAAGTGCA AGATCAGCAC TTATTGATTT TGCACATCAT 
ATTACTAAAA ATAATAGTTT ATTTATTTGT GGCCATATTA TTGAGACACC AATATCATAT 
AAGACGCGAA ATTCCATGAT TGCGAATTGT ACTTCCTGGT TTAGAGCAAA TAAAATTAAG 
GCATTTTATT CTCTGGTGGA TGGTGCAAAT TTTCAAGACG GCACTACTTC TCTTTTACAA 
GCTGCCGGTT TAGGAAAAAT GAAGCCTAAT ATTTTATTAA TGGGCTATAA GCAAGACTGG 
GCAACTTGTC CTCGAGAAAA TTTAAATATG TACTTTAATA TAATGCACAA AGCTTTAGAT 
ATGCATATAG CAGTAGCTCT TCTTCGAATA AGTGAAGGTT TAGATTGTAG TATAGGAGAT 
GTTGAAGAAC AAAAGAAAAG TATCCAAACA ATTCCAGGGA ATCAAAGCTT TTCTCAACTT 
AGCCAAGCCA GTAGTACATC AGATATTTCT ATTCCTGGAA GTCCAGCTCC AAGAAGATCT 
AAAATGATTA ATGAATATCC TAATCCAACT GCAGAAGATC AACGTGAAAA TCGACAAAAT 
TTCTTACCCA TAGCGAAAGA TATACTTAAT GCTGTAACAA AATTTCAACG TAAACAAAAA 
AAGGGTACTA TAGATGTATG GTGGTTGTAT GATGATGGTG GTTTAACTCT TTTATTACCT 
TATATTATCA GTACAAGACG TAATTGGTCT AATAATAAAT TAAGAGTTTT TGCTTTAGCA 
AATAAAAATT CTGAACTCGA ATATGAACAA AGAAATATGG CAAGTCTTTT ATCGAAATTC 
AGGATAGATT ATTCTGCATT GAAAGTTATA CCAGATATTT CAAAACCAGC ACAGAATACT 
ACCAAAAGTT TCTTTGATTC ATTAATAGCA AATTTCCAAG AAACAGAAAA TACAAAAACA 
ATGAATGATG ATATTATCAA AGATTCTGAA TTAATTGCTA TGAAAGAAAA AACAAATAGA 
CATCTACGTT TAAGAGAATT ATTACTTGAA AATTCTATGG ACGCTAATTT AGTAGTTATG 
ACATTGCCAA TGCCTCGAAA GGGTGCTGTT TCAGCACCTC TCTATATGGC TTGGCTCGAA 
ACTTTAACGC GTGATATGCC ACCATTTTTA CTTGTTCGAG GAAATCACAC ATCTGTTTTA 
ACTTTTTACT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016922484.1
CDS	517..3651
Translation	MAHENGISGDTDSETVELNPLRRTRFHVNRVDSLEGRASLLGEQETRKSLRHMTREALPRLDNYRNIMSIQAAHRPSLDELHNPTVLNKGAGSHNLPPVQVKPPTSGIKFGWIQGVLLRCLLNIWGVMLFLRLSWVVAQAGIGEAILLILTTTTVTTITSLSMSAISTNGLIKGGGTYYMISRSLGPEFGGSIGLIFSLANAVACSMYVVGFCESMVDCLKSNGVCIVDCDNTDIRIIGCITIVLLLLIVMIGLEWEAKAQIGLLIILLLAIADFMIGTFIGPKDDQERAKGFMGYNADLFKENFYPDYRYSEGMKHNFFSVLAIFFPAATGILAGANISGDLKDPQTAIPKGTLLAILLTTISYLFMALMVGGSVMRDASGDVNDLWNIFNSSYTALSSSNTTNETTESSIIVNSNQIVWSIYSTKFNCTGNCQYGSHNSFQVIELVSAFGPFIYAGCFAATLSSALASLVSAPKVFQALCLDKLYPGIAWFSGDKDKEPIRGYLLTFIIAVGFILIGELNAIAPLISNFFLAAYTLINFSTFHASLAKPIGWRPTFKYYNMWLSLAGAILCVSVMFLISWWTALITLFVVLTLYLIVSYRKPDVNWGSTTQAQTYNNALNAVQQLDRVEEHVKNYRPQLLVLSGTPSARSALIDFAHHITKNNSLFICGHIIETPISYKTRNSMIANCTSWFRANKIKAFYSLVDGANFQDGTTSLLQAAGLGKMKPNILLMGYKQDWATCPRENLNMYFNIMHKALDMHIAVALLRISEGLDCSIGDVEEQKKSIQTIPGNQSFSQLSQASSTSDISIPGSPAPRRSKMINEYPNPTAEDQRENRQNFLPIAKDILNAVTKFQRKQKKGTIDVWWLYDDGGLTLLLPYIISTRRNWSNNKLRVFALANKNSELEYEQRNMASLLSKFRIDYSALKVIPDISKPAQNTTKSFFDSLIANFQETENTKTMNDDIIKDSELIAMKEKTNRHLRLRELLLENSMDANLVVMTLPMPRKGAVSAPLYMAWLETLTRDMPPFLLVRGNHTSVLTFYS

Target ORF information:

RefSeq Version	XM_017066995.2
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana bumetanide-sensitive sodium-(potassium)-chloride cotransporter (LOC108004236), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017066995.2

ORF Insert Sequence:

ATGGCACATG AAAATGGTAT AAGCGGTGAT ACCGATTCCG AAACGGTAGA ATTGAATCCA 
TTGAGAAGAA CCAGATTCCA TGTAAATCGA GTGGACAGTC TTGAAGGTCG AGCGAGCCTT 
CTTGGAGAAC AAGAAACGAG AAAGTCCCTC CGACACATGA CTAGGGAGGC ACTGCCTAGA 
CTGGATAATT ATAGAAACAT TATGAGTATC CAGGCCGCTC ATAGACCGTC TTTGGACGAG 
CTTCACAATC CCACTGTGCT TAACAAGGGT GCAGGATCGC ATAATTTGCC ACCAGTGCAA 
GTGAAGCCAC CGACTTCCGG AATAAAATTC GGATGGATTC AAGGAGTTTT ATTAAGATGC 
CTTCTCAATA TTTGGGGGGT AATGCTTTTT TTACGTCTCT CCTGGGTCGT AGCACAAGCA 
GGCATAGGAG AAGCTATTTT ATTGATTTTA ACAACGACAA CTGTGACTAC GATAACATCG 
TTATCTATGT CGGCAATTAG CACAAATGGT CTCATTAAAG GAGGTGGCAC GTATTACATG 
ATTTCCAGAT CACTGGGGCC AGAATTCGGA GGATCTATAG GACTTATATT TTCTTTGGCG 
AACGCGGTCG CTTGTTCCAT GTATGTTGTT GGATTTTGCG AGAGTATGGT CGATTGCTTA 
AAATCAAATG GAGTTTGTAT TGTTGATTGC GACAATACGG ATATTAGAAT TATAGGTTGT 
ATCACGATAG TTCTGTTACT TTTGATCGTT ATGATTGGAT TGGAATGGGA AGCCAAAGCT 
CAAATTGGTT TGCTGATTAT ATTGCTTTTA GCTATCGCTG ATTTTATGAT CGGCACCTTT 
ATTGGACCTA AAGATGATCA AGAACGGGCT AAAGGATTTA TGGGATATAA TGCTGATCTA 
TTCAAGGAGA ACTTTTATCC GGATTATAGA TATAGTGAGG GAATGAAGCA TAATTTTTTT 
TCAGTCTTGG CTATTTTTTT CCCTGCGGCT ACAGGTATCC TGGCAGGTGC GAATATATCC 
GGTGATTTGA AGGATCCGCA AACAGCTATT CCTAAAGGAA CATTGTTAGC TATTCTTTTA 
ACAACTATAT CTTACCTCTT TATGGCACTT ATGGTGGGCG GCTCAGTTAT GCGAGATGCA 
TCTGGCGATG TTAATGATCT GTGGAACATT TTCAATAGTT CATATACTGC CTTATCAAGT 
TCGAATACGA CTAATGAAAC AACTGAATCT TCAATTATTG TAAATTCCAA TCAAATAGTA 
TGGAGTATAT ATAGTACAAA ATTTAATTGT ACAGGAAATT GTCAATATGG TAGCCATAAT 
AGTTTTCAGG TGATCGAATT GGTTTCTGCA TTTGGACCTT TTATTTATGC TGGATGTTTT 
GCAGCTACTT TATCAAGCGC TTTAGCATCA TTAGTTTCCG CTCCCAAAGT ATTTCAGGCA 
CTTTGTCTTG ATAAATTATA TCCTGGAATA GCATGGTTTA GTGGCGACAA AGATAAAGAA 
CCAATTCGTG GTTATCTTCT TACTTTTATA ATCGCAGTTG GTTTTATTTT AATCGGAGAA 
TTGAATGCTA TAGCTCCTTT GATCTCAAAT TTTTTCTTGG CTGCCTATAC TCTTATTAAT 
TTTAGCACTT TTCATGCTTC GTTGGCAAAA CCAATTGGTT GGAGACCAAC GTTTAAATAT 
TATAATATGT GGCTAAGTCT TGCTGGTGCT ATTCTCTGTG TTTCTGTGAT GTTCTTGATC 
TCATGGTGGA CAGCGTTGAT CACATTATTT GTAGTATTAA CACTTTATTT AATAGTTTCT 
TATAGAAAAC CAGATGTTAA TTGGGGTTCT ACAACACAAG CCCAAACATA TAATAATGCC 
TTAAATGCTG TTCAACAATT AGATAGAGTT GAAGAACATG TTAAAAATTA TAGACCTCAA 
TTGTTAGTAT TAAGTGGCAC TCCAAGTGCA AGATCAGCAC TTATTGATTT TGCACATCAT 
ATTACTAAAA ATAATAGTTT ATTTATTTGT GGCCATATTA TTGAGACACC AATATCATAT 
AAGACGCGAA ATTCCATGAT TGCGAATTGT ACTTCCTGGT TTAGAGCAAA TAAAATTAAG 
GCATTTTATT CTCTGGTGGA TGGTGCAAAT TTTCAAGACG GCACTACTTC TCTTTTACAA 
GCTGCCGGTT TAGGAAAAAT GAAGCCTAAT ATTTTATTAA TGGGCTATAA GCAAGACTGG 
GCAACTTGTC CTCGAGAAAA TTTAAATATG TACTTTAATA TAATGCACAA AGCTTTAGAT 
ATGCATATAG CAGTAGCTCT TCTTCGAATA AGTGAAGGTT TAGATTGTAG TATAGGAGAT 
GTTGAAGAAC AAAAGAAAAG TATCCAAACA ATTCCAGGGA ATCAAAGCTT TTCTCAACTT 
AGCCAAGCCA GTAGTACATC AGATATTTCT ATTCCTGGAA GTCCAGCTCC AAGAAGATCT 
AAAATGATTA ATGAATATCC TAATCCAACT GCAGAAGATC AACGTGAAAA TCGACAAAAT 
TTCTTACCCA TAGCGAAAGA TATACTTAAT GCTGTAACAA AATTTCAACG TAAACAAAAA 
AAGGGTACTA TAGATGTATG GTGGTTGTAT GATGATGGTG GTTTAACTCT TTTATTACCT 
TATATTATCA GTACAAGACG TAATTGGTCT AATAATAAAT TAAGAGTTTT TGCTTTAGCA 
AATAAAAATT CTGAACTCGA ATATGAACAA AGAAATATGG CAAGTCTTTT ATCGAAATTC 
AGGATAGATT ATTCTGCATT GAAAGTTATA CCAGATATTT CAAAACCAGC ACAGAATACT 
ACCAAAAGTT TCTTTGATTC ATTAATAGCA AATTTCCAAG AAACAGAAAA TACAAAAACA 
ATGAATGATG ATATTATCAA AGATTCTGAA TTAATTGCTA TGAAAGAAAA AACAAATAGA 
CATCTACGTT TAAGAGAATT ATTACTTGAA AATTCTATGG ACGCTAATTT AGTAGTTATG 
ACATTGCCAA TGCCTCGAAA GGGTGCTGTT TCAGCACCTC TCTATATGGC TTGGCTCGAA 
ACTTTAACGC GTGATATGCC ACCATTTTTA CTTGTTCGAG GAAATCACAC ATCTGTTTTA 
ACTTTTTACT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108004236 ( XM_017066993.2 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_016922482.1 Apis cerana bumetanide-sensitive sodium-(potassium)-chloride cotransporter (LOC108004236), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OAp30532
Clone ID Related Accession (Same CDS sequence)	XM_017066995.1 , XM_017066993.1 , XM_017066994.2 , XM_017066994.1 , XM_017066995.2 , XM_017066993.2
Accession Version	XM_017066993.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-16
Organism	Apis cerana(Asiatic honeybee)
Product	bumetanide-sensitive sodium-(potassium)-chloride cotransporter
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016017734.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 17, 2019 this sequence version replaced XM_017066993.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis cerana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACATG AAAATGGTAT AAGCGGTGAT ACCGATTCCG AAACGGTAGA ATTGAATCCA 
TTGAGAAGAA CCAGATTCCA TGTAAATCGA GTGGACAGTC TTGAAGGTCG AGCGAGCCTT 
CTTGGAGAAC AAGAAACGAG AAAGTCCCTC CGACACATGA CTAGGGAGGC ACTGCCTAGA 
CTGGATAATT ATAGAAACAT TATGAGTATC CAGGCCGCTC ATAGACCGTC TTTGGACGAG 
CTTCACAATC CCACTGTGCT TAACAAGGGT GCAGGATCGC ATAATTTGCC ACCAGTGCAA 
GTGAAGCCAC CGACTTCCGG AATAAAATTC GGATGGATTC AAGGAGTTTT ATTAAGATGC 
CTTCTCAATA TTTGGGGGGT AATGCTTTTT TTACGTCTCT CCTGGGTCGT AGCACAAGCA 
GGCATAGGAG AAGCTATTTT ATTGATTTTA ACAACGACAA CTGTGACTAC GATAACATCG 
TTATCTATGT CGGCAATTAG CACAAATGGT CTCATTAAAG GAGGTGGCAC GTATTACATG 
ATTTCCAGAT CACTGGGGCC AGAATTCGGA GGATCTATAG GACTTATATT TTCTTTGGCG 
AACGCGGTCG CTTGTTCCAT GTATGTTGTT GGATTTTGCG AGAGTATGGT CGATTGCTTA 
AAATCAAATG GAGTTTGTAT TGTTGATTGC GACAATACGG ATATTAGAAT TATAGGTTGT 
ATCACGATAG TTCTGTTACT TTTGATCGTT ATGATTGGAT TGGAATGGGA AGCCAAAGCT 
CAAATTGGTT TGCTGATTAT ATTGCTTTTA GCTATCGCTG ATTTTATGAT CGGCACCTTT 
ATTGGACCTA AAGATGATCA AGAACGGGCT AAAGGATTTA TGGGATATAA TGCTGATCTA 
TTCAAGGAGA ACTTTTATCC GGATTATAGA TATAGTGAGG GAATGAAGCA TAATTTTTTT 
TCAGTCTTGG CTATTTTTTT CCCTGCGGCT ACAGGTATCC TGGCAGGTGC GAATATATCC 
GGTGATTTGA AGGATCCGCA AACAGCTATT CCTAAAGGAA CATTGTTAGC TATTCTTTTA 
ACAACTATAT CTTACCTCTT TATGGCACTT ATGGTGGGCG GCTCAGTTAT GCGAGATGCA 
TCTGGCGATG TTAATGATCT GTGGAACATT TTCAATAGTT CATATACTGC CTTATCAAGT 
TCGAATACGA CTAATGAAAC AACTGAATCT TCAATTATTG TAAATTCCAA TCAAATAGTA 
TGGAGTATAT ATAGTACAAA ATTTAATTGT ACAGGAAATT GTCAATATGG TAGCCATAAT 
AGTTTTCAGG TGATCGAATT GGTTTCTGCA TTTGGACCTT TTATTTATGC TGGATGTTTT 
GCAGCTACTT TATCAAGCGC TTTAGCATCA TTAGTTTCCG CTCCCAAAGT ATTTCAGGCA 
CTTTGTCTTG ATAAATTATA TCCTGGAATA GCATGGTTTA GTGGCGACAA AGATAAAGAA 
CCAATTCGTG GTTATCTTCT TACTTTTATA ATCGCAGTTG GTTTTATTTT AATCGGAGAA 
TTGAATGCTA TAGCTCCTTT GATCTCAAAT TTTTTCTTGG CTGCCTATAC TCTTATTAAT 
TTTAGCACTT TTCATGCTTC GTTGGCAAAA CCAATTGGTT GGAGACCAAC GTTTAAATAT 
TATAATATGT GGCTAAGTCT TGCTGGTGCT ATTCTCTGTG TTTCTGTGAT GTTCTTGATC 
TCATGGTGGA CAGCGTTGAT CACATTATTT GTAGTATTAA CACTTTATTT AATAGTTTCT 
TATAGAAAAC CAGATGTTAA TTGGGGTTCT ACAACACAAG CCCAAACATA TAATAATGCC 
TTAAATGCTG TTCAACAATT AGATAGAGTT GAAGAACATG TTAAAAATTA TAGACCTCAA 
TTGTTAGTAT TAAGTGGCAC TCCAAGTGCA AGATCAGCAC TTATTGATTT TGCACATCAT 
ATTACTAAAA ATAATAGTTT ATTTATTTGT GGCCATATTA TTGAGACACC AATATCATAT 
AAGACGCGAA ATTCCATGAT TGCGAATTGT ACTTCCTGGT TTAGAGCAAA TAAAATTAAG 
GCATTTTATT CTCTGGTGGA TGGTGCAAAT TTTCAAGACG GCACTACTTC TCTTTTACAA 
GCTGCCGGTT TAGGAAAAAT GAAGCCTAAT ATTTTATTAA TGGGCTATAA GCAAGACTGG 
GCAACTTGTC CTCGAGAAAA TTTAAATATG TACTTTAATA TAATGCACAA AGCTTTAGAT 
ATGCATATAG CAGTAGCTCT TCTTCGAATA AGTGAAGGTT TAGATTGTAG TATAGGAGAT 
GTTGAAGAAC AAAAGAAAAG TATCCAAACA ATTCCAGGGA ATCAAAGCTT TTCTCAACTT 
AGCCAAGCCA GTAGTACATC AGATATTTCT ATTCCTGGAA GTCCAGCTCC AAGAAGATCT 
AAAATGATTA ATGAATATCC TAATCCAACT GCAGAAGATC AACGTGAAAA TCGACAAAAT 
TTCTTACCCA TAGCGAAAGA TATACTTAAT GCTGTAACAA AATTTCAACG TAAACAAAAA 
AAGGGTACTA TAGATGTATG GTGGTTGTAT GATGATGGTG GTTTAACTCT TTTATTACCT 
TATATTATCA GTACAAGACG TAATTGGTCT AATAATAAAT TAAGAGTTTT TGCTTTAGCA 
AATAAAAATT CTGAACTCGA ATATGAACAA AGAAATATGG CAAGTCTTTT ATCGAAATTC 
AGGATAGATT ATTCTGCATT GAAAGTTATA CCAGATATTT CAAAACCAGC ACAGAATACT 
ACCAAAAGTT TCTTTGATTC ATTAATAGCA AATTTCCAAG AAACAGAAAA TACAAAAACA 
ATGAATGATG ATATTATCAA AGATTCTGAA TTAATTGCTA TGAAAGAAAA AACAAATAGA 
CATCTACGTT TAAGAGAATT ATTACTTGAA AATTCTATGG ACGCTAATTT AGTAGTTATG 
ACATTGCCAA TGCCTCGAAA GGGTGCTGTT TCAGCACCTC TCTATATGGC TTGGCTCGAA 
ACTTTAACGC GTGATATGCC ACCATTTTTA CTTGTTCGAG GAAATCACAC ATCTGTTTTA 
ACTTTTTACT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016922482.1
CDS	846..3980
Translation	MAHENGISGDTDSETVELNPLRRTRFHVNRVDSLEGRASLLGEQETRKSLRHMTREALPRLDNYRNIMSIQAAHRPSLDELHNPTVLNKGAGSHNLPPVQVKPPTSGIKFGWIQGVLLRCLLNIWGVMLFLRLSWVVAQAGIGEAILLILTTTTVTTITSLSMSAISTNGLIKGGGTYYMISRSLGPEFGGSIGLIFSLANAVACSMYVVGFCESMVDCLKSNGVCIVDCDNTDIRIIGCITIVLLLLIVMIGLEWEAKAQIGLLIILLLAIADFMIGTFIGPKDDQERAKGFMGYNADLFKENFYPDYRYSEGMKHNFFSVLAIFFPAATGILAGANISGDLKDPQTAIPKGTLLAILLTTISYLFMALMVGGSVMRDASGDVNDLWNIFNSSYTALSSSNTTNETTESSIIVNSNQIVWSIYSTKFNCTGNCQYGSHNSFQVIELVSAFGPFIYAGCFAATLSSALASLVSAPKVFQALCLDKLYPGIAWFSGDKDKEPIRGYLLTFIIAVGFILIGELNAIAPLISNFFLAAYTLINFSTFHASLAKPIGWRPTFKYYNMWLSLAGAILCVSVMFLISWWTALITLFVVLTLYLIVSYRKPDVNWGSTTQAQTYNNALNAVQQLDRVEEHVKNYRPQLLVLSGTPSARSALIDFAHHITKNNSLFICGHIIETPISYKTRNSMIANCTSWFRANKIKAFYSLVDGANFQDGTTSLLQAAGLGKMKPNILLMGYKQDWATCPRENLNMYFNIMHKALDMHIAVALLRISEGLDCSIGDVEEQKKSIQTIPGNQSFSQLSQASSTSDISIPGSPAPRRSKMINEYPNPTAEDQRENRQNFLPIAKDILNAVTKFQRKQKKGTIDVWWLYDDGGLTLLLPYIISTRRNWSNNKLRVFALANKNSELEYEQRNMASLLSKFRIDYSALKVIPDISKPAQNTTKSFFDSLIANFQETENTKTMNDDIIKDSELIAMKEKTNRHLRLRELLLENSMDANLVVMTLPMPRKGAVSAPLYMAWLETLTRDMPPFLLVRGNHTSVLTFYS

Target ORF information:

RefSeq Version	XM_017066993.2
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana bumetanide-sensitive sodium-(potassium)-chloride cotransporter (LOC108004236), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017066993.2

ORF Insert Sequence:

ATGGCACATG AAAATGGTAT AAGCGGTGAT ACCGATTCCG AAACGGTAGA ATTGAATCCA 
TTGAGAAGAA CCAGATTCCA TGTAAATCGA GTGGACAGTC TTGAAGGTCG AGCGAGCCTT 
CTTGGAGAAC AAGAAACGAG AAAGTCCCTC CGACACATGA CTAGGGAGGC ACTGCCTAGA 
CTGGATAATT ATAGAAACAT TATGAGTATC CAGGCCGCTC ATAGACCGTC TTTGGACGAG 
CTTCACAATC CCACTGTGCT TAACAAGGGT GCAGGATCGC ATAATTTGCC ACCAGTGCAA 
GTGAAGCCAC CGACTTCCGG AATAAAATTC GGATGGATTC AAGGAGTTTT ATTAAGATGC 
CTTCTCAATA TTTGGGGGGT AATGCTTTTT TTACGTCTCT CCTGGGTCGT AGCACAAGCA 
GGCATAGGAG AAGCTATTTT ATTGATTTTA ACAACGACAA CTGTGACTAC GATAACATCG 
TTATCTATGT CGGCAATTAG CACAAATGGT CTCATTAAAG GAGGTGGCAC GTATTACATG 
ATTTCCAGAT CACTGGGGCC AGAATTCGGA GGATCTATAG GACTTATATT TTCTTTGGCG 
AACGCGGTCG CTTGTTCCAT GTATGTTGTT GGATTTTGCG AGAGTATGGT CGATTGCTTA 
AAATCAAATG GAGTTTGTAT TGTTGATTGC GACAATACGG ATATTAGAAT TATAGGTTGT 
ATCACGATAG TTCTGTTACT TTTGATCGTT ATGATTGGAT TGGAATGGGA AGCCAAAGCT 
CAAATTGGTT TGCTGATTAT ATTGCTTTTA GCTATCGCTG ATTTTATGAT CGGCACCTTT 
ATTGGACCTA AAGATGATCA AGAACGGGCT AAAGGATTTA TGGGATATAA TGCTGATCTA 
TTCAAGGAGA ACTTTTATCC GGATTATAGA TATAGTGAGG GAATGAAGCA TAATTTTTTT 
TCAGTCTTGG CTATTTTTTT CCCTGCGGCT ACAGGTATCC TGGCAGGTGC GAATATATCC 
GGTGATTTGA AGGATCCGCA AACAGCTATT CCTAAAGGAA CATTGTTAGC TATTCTTTTA 
ACAACTATAT CTTACCTCTT TATGGCACTT ATGGTGGGCG GCTCAGTTAT GCGAGATGCA 
TCTGGCGATG TTAATGATCT GTGGAACATT TTCAATAGTT CATATACTGC CTTATCAAGT 
TCGAATACGA CTAATGAAAC AACTGAATCT TCAATTATTG TAAATTCCAA TCAAATAGTA 
TGGAGTATAT ATAGTACAAA ATTTAATTGT ACAGGAAATT GTCAATATGG TAGCCATAAT 
AGTTTTCAGG TGATCGAATT GGTTTCTGCA TTTGGACCTT TTATTTATGC TGGATGTTTT 
GCAGCTACTT TATCAAGCGC TTTAGCATCA TTAGTTTCCG CTCCCAAAGT ATTTCAGGCA 
CTTTGTCTTG ATAAATTATA TCCTGGAATA GCATGGTTTA GTGGCGACAA AGATAAAGAA 
CCAATTCGTG GTTATCTTCT TACTTTTATA ATCGCAGTTG GTTTTATTTT AATCGGAGAA 
TTGAATGCTA TAGCTCCTTT GATCTCAAAT TTTTTCTTGG CTGCCTATAC TCTTATTAAT 
TTTAGCACTT TTCATGCTTC GTTGGCAAAA CCAATTGGTT GGAGACCAAC GTTTAAATAT 
TATAATATGT GGCTAAGTCT TGCTGGTGCT ATTCTCTGTG TTTCTGTGAT GTTCTTGATC 
TCATGGTGGA CAGCGTTGAT CACATTATTT GTAGTATTAA CACTTTATTT AATAGTTTCT 
TATAGAAAAC CAGATGTTAA TTGGGGTTCT ACAACACAAG CCCAAACATA TAATAATGCC 
TTAAATGCTG TTCAACAATT AGATAGAGTT GAAGAACATG TTAAAAATTA TAGACCTCAA 
TTGTTAGTAT TAAGTGGCAC TCCAAGTGCA AGATCAGCAC TTATTGATTT TGCACATCAT 
ATTACTAAAA ATAATAGTTT ATTTATTTGT GGCCATATTA TTGAGACACC AATATCATAT 
AAGACGCGAA ATTCCATGAT TGCGAATTGT ACTTCCTGGT TTAGAGCAAA TAAAATTAAG 
GCATTTTATT CTCTGGTGGA TGGTGCAAAT TTTCAAGACG GCACTACTTC TCTTTTACAA 
GCTGCCGGTT TAGGAAAAAT GAAGCCTAAT ATTTTATTAA TGGGCTATAA GCAAGACTGG 
GCAACTTGTC CTCGAGAAAA TTTAAATATG TACTTTAATA TAATGCACAA AGCTTTAGAT 
ATGCATATAG CAGTAGCTCT TCTTCGAATA AGTGAAGGTT TAGATTGTAG TATAGGAGAT 
GTTGAAGAAC AAAAGAAAAG TATCCAAACA ATTCCAGGGA ATCAAAGCTT TTCTCAACTT 
AGCCAAGCCA GTAGTACATC AGATATTTCT ATTCCTGGAA GTCCAGCTCC AAGAAGATCT 
AAAATGATTA ATGAATATCC TAATCCAACT GCAGAAGATC AACGTGAAAA TCGACAAAAT 
TTCTTACCCA TAGCGAAAGA TATACTTAAT GCTGTAACAA AATTTCAACG TAAACAAAAA 
AAGGGTACTA TAGATGTATG GTGGTTGTAT GATGATGGTG GTTTAACTCT TTTATTACCT 
TATATTATCA GTACAAGACG TAATTGGTCT AATAATAAAT TAAGAGTTTT TGCTTTAGCA 
AATAAAAATT CTGAACTCGA ATATGAACAA AGAAATATGG CAAGTCTTTT ATCGAAATTC 
AGGATAGATT ATTCTGCATT GAAAGTTATA CCAGATATTT CAAAACCAGC ACAGAATACT 
ACCAAAAGTT TCTTTGATTC ATTAATAGCA AATTTCCAAG AAACAGAAAA TACAAAAACA 
ATGAATGATG ATATTATCAA AGATTCTGAA TTAATTGCTA TGAAAGAAAA AACAAATAGA 
CATCTACGTT TAAGAGAATT ATTACTTGAA AATTCTATGG ACGCTAATTT AGTAGTTATG 
ACATTGCCAA TGCCTCGAAA GGGTGCTGTT TCAGCACCTC TCTATATGGC TTGGCTCGAA 
ACTTTAACGC GTGATATGCC ACCATTTTTA CTTGTTCGAG GAAATCACAC ATCTGTTTTA 
ACTTTTTACT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

LOC108004236 cDNA ORF clone, Apis cerana(Asiatic honeybee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム