LOC108003564 gene cDNA ORF clone, Apis cerana(Asiatic honeybee)

mRNA	Protein	Name
XM_017065895.1	XP_016921384.1	protein lap4 isoform X8
XM_017065891.2	XP_016921380.1	protein lap4 isoform X5
XM_017065887.1	XP_016921376.1	protein lap4 isoform X1
XM_017065892.1	XP_016921381.1	protein lap4 isoform X6
XM_017065890.2	XP_016921379.1	protein lap4 isoform X4
XM_017065898.2	XP_016921387.1	protein lap4 isoform X10
XM_017065888.1	XP_016921377.1	protein lap4 isoform X2
XM_017065894.1	XP_016921383.1	protein lap4 isoform X7
XM_017065897.1	XP_016921386.1	protein lap4 isoform X10
XM_017065898.1	XP_016921387.1	protein lap4 isoform X11
XM_017065889.2	XP_016921378.1	protein lap4 isoform X3
XM_017065889.1	XP_016921378.1	protein lap4 isoform X3
XM_017065896.1	XP_016921385.1	protein lap4 isoform X9
XM_017065887.2	XP_016921376.1	protein lap4 isoform X1
XM_017065892.2	XP_016921381.1	protein lap4 isoform X6
XM_017065897.2	XP_016921386.1	protein lap4 isoform X9
XM_017065890.1	XP_016921379.1	protein lap4 isoform X4
XM_017065891.1	XP_016921380.1	protein lap4 isoform X5
XM_017065888.2	XP_016921377.1	protein lap4 isoform X2
XM_017065894.2	XP_016921383.1	protein lap4 isoform X7
XM_017065895.2	XP_016921384.1	protein lap4 isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAp29726	XM_017065892.2 Latest version!	Apis cerana protein lap4 (LOC108003564), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OAp29727	XM_017065894.2 Latest version!	Apis cerana protein lap4 (LOC108003564), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OAp29728	XM_017065895.2 Latest version!	Apis cerana protein lap4 (LOC108003564), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OAp29729	XM_017065896.1 Latest version!	Apis cerana protein lap4 (LOC108003564), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OAp29730	XM_017065897.2 Latest version!	Apis cerana protein lap4 (LOC108003564), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OAp29731	XM_017065898.2 Latest version!	Apis cerana protein lap4 (LOC108003564), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAp29721	XM_017065887.2 Latest version!	Apis cerana protein lap4 (LOC108003564), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OAp29722	XM_017065888.2 Latest version!	Apis cerana protein lap4 (LOC108003564), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OAp29723	XM_017065889.2 Latest version!	Apis cerana protein lap4 (LOC108003564), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OAp29724	XM_017065890.2 Latest version!	Apis cerana protein lap4 (LOC108003564), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OAp29725	XM_017065891.2 Latest version!	Apis cerana protein lap4 (LOC108003564), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
View Old Accession Versions >>
OAp29731	XM_017065898.1	Apis cerana protein lap4 (LOC108003564), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAp29723	XM_017065889.1	Apis cerana protein lap4 (LOC108003564), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OAp29721	XM_017065887.1	Apis cerana protein lap4 (LOC108003564), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OAp29726	XM_017065892.1	Apis cerana protein lap4 (LOC108003564), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OAp29730	XM_017065897.1	Apis cerana protein lap4 (LOC108003564), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OAp29724	XM_017065890.1	Apis cerana protein lap4 (LOC108003564), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OAp29725	XM_017065891.1	Apis cerana protein lap4 (LOC108003564), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OAp29722	XM_017065888.1	Apis cerana protein lap4 (LOC108003564), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OAp29727	XM_017065894.1	Apis cerana protein lap4 (LOC108003564), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OAp29728	XM_017065895.1	Apis cerana protein lap4 (LOC108003564), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

LOC108003564 ( XM_017065892.1 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_016921381.1 Apis cerana protein lap4 (LOC108003564), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OAp29726
Clone ID Related Accession (Same CDS sequence)	XM_017065892.1 , XM_017065892.2
Accession Version	XM_017065892.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-08
Organism	Apis cerana(Asiatic honeybee)
Product	protein lap4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016017567.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis cerana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGC AGATATCATC GAGAAAAAAC 
TCAGTGTGCT CTAGTTTAAG TACTAGTAGA GCTCCAAGTG CAACATCTCA TGTTTCATCT 
ATAGGTTTTT CACATAATTT AGAAAATGGT GATGCTTTAT CTCATGATGC TATTAAGTGT 
AAGAAAATTC CTGAACCTAT ATCTTCAACA AAAGTAGGCA ATGAACCAAT GGTATCAGTT 
CTTGTTCACA CAACATTAAT ACGGGACCAA AATGGGCTTG GATTTAGTAT TGCTGGTGGA 
GAAGGTTCTC CACCATTCAA AGACAATAGT GATGCGATAT ATATTTCAAG AATAACAGAT 
GGCGGCGTAG CACAAAAAGA TGGTAAATTA TTAGTTGGAG ACAAAGTAAT ATCTATTAAT 
GGAGTTGAGA TGAGAGGAGC TAAGCATGAA CAAGCAGTTG CTTTATTAAC AGGTTTAGAA 
AGATTTGTTC GATTAGTAGT TGAACGCGAA ATACCATTTT CGCAAGCAAA TGCAGCTACG 
GTCGTAACAC CTTCAGAAAA ATCACCACGA GTGATCGGTG CACCTAAACC ATATACAGGA 
TTATATAATG CCAATAGTTA TATAGCAAAT AAACCGGGAT ATACTGGATA CAGACGTTCT 
ATAGATGCTG ATAGAACATT ATCTCCAAGC CCTACTTCGA AAACGCCTCC ACCTATGAAA 
ATTGAAACTA CTGAATTACC GAAAATGAAC GGTGTTACAG AATCAGGAAA TATTAAAAAT 
GTTTCTTCGA TATCACCAAG TCCAACAAAT AGCCAACTAC ATCCACAGCC TGCTCCTAGG 
CACAGCATTT CGCAACCAAC AATTACACCT ACAACTACTA CGAGTTCAAC GCCCACATCT 
TCTACAGAAC CTAGGTCAAC CTCACCCCAG GAAGATATAC AGGTACCCAA GCCTATCACC 
AATGAAGAAT TTCAGGCTAT GATTCCTGCT CATTTCCTTC GTCCTCCTAC TTCCTCACCA 
TCGCCAGATT CCCATCAAGG CCCTATCGTG ACAGTGACAA TAAAACAGCC TGATAATCTA 
CCTGGAGATG TTAATTTTCC TCCGGCTCCT ACAACACTTG GTAAAGTTAC TGAGACCATC 
ACAAAGAGCA CTCTCACCGA GACCGTCGTA ACTAGGGTGA CTGAAAATCA ATTGGTACCA 
CCAGTAATTA TTGAGGACGT GATTCTTGTA AAAGAGGGAT CTCTAGGATT TAGTATTATT 
GGTGGTACGG ATCATTCATG CACTCCATTT GGTGCAAAGG AACCTGGAAT ATTTATATCT 
CATGTCGTGC CTGGTGGTAT AGCTGCAAAA TCTGGTAAAT TAAGAATGGG TGATAGAATA 
CTAAAGGTAA ATGGTACTGA TGTAACAAAA GCTACTCATC AAGAGGCTGT GATGGAACTT 
TTGCGACCAG GAGATCAAAT AGTGCTTACT GTCCAACACG ATCCACTACC CGAAAATTAT 
CAGCTGGTCG AAATAGAGTA CATCCCTGTG ACAGAATTAG TTATTACAAA AGAACCAGGA 
GAGAAACTGG GCATGCATAT TAAAGGTGGA CTTCGAGGAC AAAAAGGTAA TCCACTTGAT 
CATACAGATG AAGGGGTTTT TATATCCAAA ATCAACTCAG GTGGTGCAGC TAAAAGAGAT 
GGAAGACTGA AAGTTGGAAT GAGACTATTA GAAGTTAATG GTACATCATT ATTAGGCGCA 
ACTCACCAAG AAGCAGTAAA TATATTGCGA TGTTCAGGAA ATACGATTAC TTTGGTGGTT 
TGTAAAGGAT ATGATAAGAG TGAAATTGAA CCAGTATTAC CAATATCCGA TGGAAGAGAT 
TCTAAGGAAT CTAGAACATC CAAAGAATTA AAGGATGCTA CAACAGAAGG TACTAAATCG 
TTATCACAAA GTATATCCAG TCTGGATCGC GATGATGAAG AAGCAGCAAC TCTTAGACAA 
GAACAGGAAA TGAAAGCTGA ACTTGTCGCA TGGGAACAAG AAGAAAGAGA ACGTGCTTTG 
CTTGAGCATA GAGAAAAATC TACTCCTGAA AAAGTATTAG ATGTAGTAAG AGCAGCTGAA 
TCATTAGTAA GCAAATCAAA TAGTCCGGTT GATATGGTCG TACCACCAAA GTCACCAGGT 
GGTACGAAAG ATCTCAAAAC AACCACTATT GTGATGAGCA AACATACTCT AGCACCTCAA 
AATACAAATT CACTGAGGAA AGAGAGAACT GGAACATCAG TGGACAATCC ATCGCCCAAG 
TCTCCGATCT CTACTTTAAC TCCATCGATG AATTTCGATC ACACTACTGC TGTCCAAAGC 
TTGCCCTCTC CAAAGAAAAA AAGTTCCATA CCAAAACGTA GTATTACATT TGCTGATCTT 
CCTCCCATTT CTAAAGAACC AATTAATTAT TCCATTTCGG CGAAAGAAGA TCCATCACCT 
TTGCATGATA GTAAATTTAC TTTCGATCCT CAAACTATTT CTTATAAGAA AGTCTATCAA 
AATCCTATCC AAGCTACTCA TTCTCGTTTC AAACTTCCTC CTACACCTTT AACTGCTCCT 
AAATCTATAG AAGATTATGG TACTTCAATT TCTTTTGGTA AACGACAGAA CGCACGCTCT 
GTTGATGGGA ATAGTCAGGT TGAGCTTTCT CCAACAACAT CACCAACACC ACCTCTAGTA 
AAGATGTCGG TTAGTGATAA AAAAAAATTG TTTGAAAGTG CCATGGAGGA ACATTTAAAA 
CCTTCCCCTA AACCAGAAAA AGTATTCAGT TTTCTAAGTC AGGATGAAGT TGAGAAAATG 
AAACAAGAAG AAGAAAAGAA GATTGCTACT CTAACGCGGG ATGAATTAAA GTCGTGGGCA 
CAACTTGATG AAAATGAAGG TTTGGAAGAT TTAGAAGAAA CATTAGAAGA TCAGGATAAT 
CGGCGACCTA ATAGCCGACT GAGTTCGCGA ACTTCGATCC CTCTTCTGCA AAATCTTCCA 
AGCAGTGTAA GGACAGCAAA AGCAGAACGC CGCTTGAAAG AAAGATTAAT ACAGGAGGGT 
ATAATTTCGG ATGAAGATGA AGAAAGTCAT TTAAGTCCTG CCGAACAGAG AGCGTTAAGA 
GCAGAAAAAC GTGCGGCATG GAGACAAGCA CGTTTGAAAT CTCTGGAACA AGATGCTCTT 
CAAGCACAGA TAGTAATTAA AAAAATGAGT GAAATGATGG ACACGAATAA AACAAGTTCA 
ATGCAGGATT CTACAGATAT TCCTATTACT CCTGTTTCTG AATCGGAAAA GACAACTGAT 
GTTGAAACTC TACGAGAAAA GATAATATCA TTGGAATTAA GTAATGCAGA AGATGAAATA 
GAAAGTATTA CCGGTACAGG AGCAAGTGAT GCTGAAAGCG AAAGCGAAGA AATTACTGTA 
CAACCTCCTA CTATGACTAC TTTACAAGAA AGAAATGAAA CAATTATATC TACAAATGAA 
GGTGCCGCAC CTAAAATAAG TGCAAATACT GTTCTTGTTT CAAAAAAAAA GAAACGAAAA 
CGTTCGAAGA AAGGTCGTCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016921381.1
CDS	233..6196
Translation	MFRCIPIFKGCNRQVEYIDKRHCSLPCVPDDILRYSRSLEELLLDANHIRDLPKNFFRLQKLRKLGLSDNEIHRLPPDIQNFENLVELDVSRNDIPDIPENIKNLRALQVADFSSNPIPRLPAGFVQLRNLTVLGLNDMSLTNLPPDFGSLEALQSLELRENLLKSLPESLSQLYKLERLDLGDNDIEVLPAHIGELPALQELWLDHNQLQHLPPEIGELKTLVCLDVSENRLEDLPEEIGGLEALTDLHLSQNVIEKLPDGLGELKKLTILKVDQNRLSTLNPNIGRCENLQELILTENFLLELPVSIGKLLNLNNLNVDRNSLQSLPTETGNLKQLGVLSLRDNKLQYLPIEVGQCTALHVLDVSGNRLQYLPYSLINLNLKAVWLSKNQAQPMLTFQTDVDEETGQEVLTCFLLPQLEIHPDDSGRLGMLAGMRNAVQDGEIRELGSSDDEGWQEKEASRTHSVKFTDEPPETDKETPFVRQNTPHPKELKAKAHKLFSKGKNDSRSASTDEQDVSQTFGLDKTETDVSTKPNDLTVDLIEQESLKHESVENEQKEIVPGVTDNIDVQSSNEPEIVSNQYIVESNTDVRTEGSISELKDRNDKEDDESENEETQRHVEFSIAEGTDYDGSGDSNRPNRLHRRDTPHHLKNKRIHTAVDKEKVASIIAQALMKKNDEISTSTSAPAETTENFDAQSQDAISDAEPTIEVREEQYEIHIERTTGGLGLSIAGGIGSTPFKGDDEGIFISRVTEGGPADLAGLKVEDKVLSVNGVSVVNVGHYDAVEVLKACGRVLVLVVQREVTRIVPPFEQQISSRKNSVCSSLSTSRAPSATSHVSSIGFSHNLENGDALSHDAIKCKKIPEPISSTKVGNEPMVSVLVHTTLIRDQNGLGFSIAGGEGSPPFKDNSDAIYISRITDGGVAQKDGKLLVGDKVISINGVEMRGAKHEQAVALLTGLERFVRLVVEREIPFSQANAATVVTPSEKSPRVIGAPKPYTGLYNANSYIANKPGYTGYRRSIDADRTLSPSPTSKTPPPMKIETTELPKMNGVTESGNIKNVSSISPSPTNSQLHPQPAPRHSISQPTITPTTTTSSTPTSSTEPRSTSPQEDIQVPKPITNEEFQAMIPAHFLRPPTSSPSPDSHQGPIVTVTIKQPDNLPGDVNFPPAPTTLGKVTETITKSTLTETVVTRVTENQLVPPVIIEDVILVKEGSLGFSIIGGTDHSCTPFGAKEPGIFISHVVPGGIAAKSGKLRMGDRILKVNGTDVTKATHQEAVMELLRPGDQIVLTVQHDPLPENYQLVEIEYIPVTELVITKEPGEKLGMHIKGGLRGQKGNPLDHTDEGVFISKINSGGAAKRDGRLKVGMRLLEVNGTSLLGATHQEAVNILRCSGNTITLVVCKGYDKSEIEPVLPISDGRDSKESRTSKELKDATTEGTKSLSQSISSLDRDDEEAATLRQEQEMKAELVAWEQEERERALLEHREKSTPEKVLDVVRAAESLVSKSNSPVDMVVPPKSPGGTKDLKTTTIVMSKHTLAPQNTNSLRKERTGTSVDNPSPKSPISTLTPSMNFDHTTAVQSLPSPKKKSSIPKRSITFADLPPISKEPINYSISAKEDPSPLHDSKFTFDPQTISYKKVYQNPIQATHSRFKLPPTPLTAPKSIEDYGTSISFGKRQNARSVDGNSQVELSPTTSPTPPLVKMSVSDKKKLFESAMEEHLKPSPKPEKVFSFLSQDEVEKMKQEEEKKIATLTRDELKSWAQLDENEGLEDLEETLEDQDNRRPNSRLSSRTSIPLLQNLPSSVRTAKAERRLKERLIQEGIISDEDEESHLSPAEQRALRAEKRAAWRQARLKSLEQDALQAQIVIKKMSEMMDTNKTSSMQDSTDIPITPVSESEKTTDVETLREKIISLELSNAEDEIESITGTGASDAESESEEITVQPPTMTTLQERNETIISTNEGAAPKISANTVLVSKKKKRKRSKKGRH

Target ORF information:

RefSeq Version	XM_017065892.1
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana protein lap4 (LOC108003564), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017065892.1

ORF Insert Sequence:

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGC AGATATCATC GAGAAAAAAC 
TCAGTGTGCT CTAGTTTAAG TACTAGTAGA GCTCCAAGTG CAACATCTCA TGTTTCATCT 
ATAGGTTTTT CACATAATTT AGAAAATGGT GATGCTTTAT CTCATGATGC TATTAAGTGT 
AAGAAAATTC CTGAACCTAT ATCTTCAACA AAAGTAGGCA ATGAACCAAT GGTATCAGTT 
CTTGTTCACA CAACATTAAT ACGGGACCAA AATGGGCTTG GATTTAGTAT TGCTGGTGGA 
GAAGGTTCTC CACCATTCAA AGACAATAGT GATGCGATAT ATATTTCAAG AATAACAGAT 
GGCGGCGTAG CACAAAAAGA TGGTAAATTA TTAGTTGGAG ACAAAGTAAT ATCTATTAAT 
GGAGTTGAGA TGAGAGGAGC TAAGCATGAA CAAGCAGTTG CTTTATTAAC AGGTTTAGAA 
AGATTTGTTC GATTAGTAGT TGAACGCGAA ATACCATTTT CGCAAGCAAA TGCAGCTACG 
GTCGTAACAC CTTCAGAAAA ATCACCACGA GTGATCGGTG CACCTAAACC ATATACAGGA 
TTATATAATG CCAATAGTTA TATAGCAAAT AAACCGGGAT ATACTGGATA CAGACGTTCT 
ATAGATGCTG ATAGAACATT ATCTCCAAGC CCTACTTCGA AAACGCCTCC ACCTATGAAA 
ATTGAAACTA CTGAATTACC GAAAATGAAC GGTGTTACAG AATCAGGAAA TATTAAAAAT 
GTTTCTTCGA TATCACCAAG TCCAACAAAT AGCCAACTAC ATCCACAGCC TGCTCCTAGG 
CACAGCATTT CGCAACCAAC AATTACACCT ACAACTACTA CGAGTTCAAC GCCCACATCT 
TCTACAGAAC CTAGGTCAAC CTCACCCCAG GAAGATATAC AGGTACCCAA GCCTATCACC 
AATGAAGAAT TTCAGGCTAT GATTCCTGCT CATTTCCTTC GTCCTCCTAC TTCCTCACCA 
TCGCCAGATT CCCATCAAGG CCCTATCGTG ACAGTGACAA TAAAACAGCC TGATAATCTA 
CCTGGAGATG TTAATTTTCC TCCGGCTCCT ACAACACTTG GTAAAGTTAC TGAGACCATC 
ACAAAGAGCA CTCTCACCGA GACCGTCGTA ACTAGGGTGA CTGAAAATCA ATTGGTACCA 
CCAGTAATTA TTGAGGACGT GATTCTTGTA AAAGAGGGAT CTCTAGGATT TAGTATTATT 
GGTGGTACGG ATCATTCATG CACTCCATTT GGTGCAAAGG AACCTGGAAT ATTTATATCT 
CATGTCGTGC CTGGTGGTAT AGCTGCAAAA TCTGGTAAAT TAAGAATGGG TGATAGAATA 
CTAAAGGTAA ATGGTACTGA TGTAACAAAA GCTACTCATC AAGAGGCTGT GATGGAACTT 
TTGCGACCAG GAGATCAAAT AGTGCTTACT GTCCAACACG ATCCACTACC CGAAAATTAT 
CAGCTGGTCG AAATAGAGTA CATCCCTGTG ACAGAATTAG TTATTACAAA AGAACCAGGA 
GAGAAACTGG GCATGCATAT TAAAGGTGGA CTTCGAGGAC AAAAAGGTAA TCCACTTGAT 
CATACAGATG AAGGGGTTTT TATATCCAAA ATCAACTCAG GTGGTGCAGC TAAAAGAGAT 
GGAAGACTGA AAGTTGGAAT GAGACTATTA GAAGTTAATG GTACATCATT ATTAGGCGCA 
ACTCACCAAG AAGCAGTAAA TATATTGCGA TGTTCAGGAA ATACGATTAC TTTGGTGGTT 
TGTAAAGGAT ATGATAAGAG TGAAATTGAA CCAGTATTAC CAATATCCGA TGGAAGAGAT 
TCTAAGGAAT CTAGAACATC CAAAGAATTA AAGGATGCTA CAACAGAAGG TACTAAATCG 
TTATCACAAA GTATATCCAG TCTGGATCGC GATGATGAAG AAGCAGCAAC TCTTAGACAA 
GAACAGGAAA TGAAAGCTGA ACTTGTCGCA TGGGAACAAG AAGAAAGAGA ACGTGCTTTG 
CTTGAGCATA GAGAAAAATC TACTCCTGAA AAAGTATTAG ATGTAGTAAG AGCAGCTGAA 
TCATTAGTAA GCAAATCAAA TAGTCCGGTT GATATGGTCG TACCACCAAA GTCACCAGGT 
GGTACGAAAG ATCTCAAAAC AACCACTATT GTGATGAGCA AACATACTCT AGCACCTCAA 
AATACAAATT CACTGAGGAA AGAGAGAACT GGAACATCAG TGGACAATCC ATCGCCCAAG 
TCTCCGATCT CTACTTTAAC TCCATCGATG AATTTCGATC ACACTACTGC TGTCCAAAGC 
TTGCCCTCTC CAAAGAAAAA AAGTTCCATA CCAAAACGTA GTATTACATT TGCTGATCTT 
CCTCCCATTT CTAAAGAACC AATTAATTAT TCCATTTCGG CGAAAGAAGA TCCATCACCT 
TTGCATGATA GTAAATTTAC TTTCGATCCT CAAACTATTT CTTATAAGAA AGTCTATCAA 
AATCCTATCC AAGCTACTCA TTCTCGTTTC AAACTTCCTC CTACACCTTT AACTGCTCCT 
AAATCTATAG AAGATTATGG TACTTCAATT TCTTTTGGTA AACGACAGAA CGCACGCTCT 
GTTGATGGGA ATAGTCAGGT TGAGCTTTCT CCAACAACAT CACCAACACC ACCTCTAGTA 
AAGATGTCGG TTAGTGATAA AAAAAAATTG TTTGAAAGTG CCATGGAGGA ACATTTAAAA 
CCTTCCCCTA AACCAGAAAA AGTATTCAGT TTTCTAAGTC AGGATGAAGT TGAGAAAATG 
AAACAAGAAG AAGAAAAGAA GATTGCTACT CTAACGCGGG ATGAATTAAA GTCGTGGGCA 
CAACTTGATG AAAATGAAGG TTTGGAAGAT TTAGAAGAAA CATTAGAAGA TCAGGATAAT 
CGGCGACCTA ATAGCCGACT GAGTTCGCGA ACTTCGATCC CTCTTCTGCA AAATCTTCCA 
AGCAGTGTAA GGACAGCAAA AGCAGAACGC CGCTTGAAAG AAAGATTAAT ACAGGAGGGT 
ATAATTTCGG ATGAAGATGA AGAAAGTCAT TTAAGTCCTG CCGAACAGAG AGCGTTAAGA 
GCAGAAAAAC GTGCGGCATG GAGACAAGCA CGTTTGAAAT CTCTGGAACA AGATGCTCTT 
CAAGCACAGA TAGTAATTAA AAAAATGAGT GAAATGATGG ACACGAATAA AACAAGTTCA 
ATGCAGGATT CTACAGATAT TCCTATTACT CCTGTTTCTG AATCGGAAAA GACAACTGAT 
GTTGAAACTC TACGAGAAAA GATAATATCA TTGGAATTAA GTAATGCAGA AGATGAAATA 
GAAAGTATTA CCGGTACAGG AGCAAGTGAT GCTGAAAGCG AAAGCGAAGA AATTACTGTA 
CAACCTCCTA CTATGACTAC TTTACAAGAA AGAAATGAAA CAATTATATC TACAAATGAA 
GGTGCCGCAC CTAAAATAAG TGCAAATACT GTTCTTGTTT CAAAAAAAAA GAAACGAAAA 
CGTTCGAAGA AAGGTCGTCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108003564 ( XM_017065892.2 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_016921381.1 Apis cerana protein lap4 (LOC108003564), transcript variant X6, mRNA.

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CloneID	OAp29726
Clone ID Related Accession (Same CDS sequence)	XM_017065892.1 , XM_017065892.2
Accession Version	XM_017065892.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-16
Organism	Apis cerana(Asiatic honeybee)
Product	protein lap4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016017567.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 17, 2019 this sequence version replaced XM_017065892.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis cerana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGC AGATATCATC GAGAAAAAAC 
TCAGTGTGCT CTAGTTTAAG TACTAGTAGA GCTCCAAGTG CAACATCTCA TGTTTCATCT 
ATAGGTTTTT CACATAATTT AGAAAATGGT GATGCTTTAT CTCATGATGC TATTAAGTGT 
AAGAAAATTC CTGAACCTAT ATCTTCAACA AAAGTAGGCA ATGAACCAAT GGTATCAGTT 
CTTGTTCACA CAACATTAAT ACGGGACCAA AATGGGCTTG GATTTAGTAT TGCTGGTGGA 
GAAGGTTCTC CACCATTCAA AGACAATAGT GATGCGATAT ATATTTCAAG AATAACAGAT 
GGCGGCGTAG CACAAAAAGA TGGTAAATTA TTAGTTGGAG ACAAAGTAAT ATCTATTAAT 
GGAGTTGAGA TGAGAGGAGC TAAGCATGAA CAAGCAGTTG CTTTATTAAC AGGTTTAGAA 
AGATTTGTTC GATTAGTAGT TGAACGCGAA ATACCATTTT CGCAAGCAAA TGCAGCTACG 
GTCGTAACAC CTTCAGAAAA ATCACCACGA GTGATCGGTG CACCTAAACC ATATACAGGA 
TTATATAATG CCAATAGTTA TATAGCAAAT AAACCGGGAT ATACTGGATA CAGACGTTCT 
ATAGATGCTG ATAGAACATT ATCTCCAAGC CCTACTTCGA AAACGCCTCC ACCTATGAAA 
ATTGAAACTA CTGAATTACC GAAAATGAAC GGTGTTACAG AATCAGGAAA TATTAAAAAT 
GTTTCTTCGA TATCACCAAG TCCAACAAAT AGCCAACTAC ATCCACAGCC TGCTCCTAGG 
CACAGCATTT CGCAACCAAC AATTACACCT ACAACTACTA CGAGTTCAAC GCCCACATCT 
TCTACAGAAC CTAGGTCAAC CTCACCCCAG GAAGATATAC AGGTACCCAA GCCTATCACC 
AATGAAGAAT TTCAGGCTAT GATTCCTGCT CATTTCCTTC GTCCTCCTAC TTCCTCACCA 
TCGCCAGATT CCCATCAAGG CCCTATCGTG ACAGTGACAA TAAAACAGCC TGATAATCTA 
CCTGGAGATG TTAATTTTCC TCCGGCTCCT ACAACACTTG GTAAAGTTAC TGAGACCATC 
ACAAAGAGCA CTCTCACCGA GACCGTCGTA ACTAGGGTGA CTGAAAATCA ATTGGTACCA 
CCAGTAATTA TTGAGGACGT GATTCTTGTA AAAGAGGGAT CTCTAGGATT TAGTATTATT 
GGTGGTACGG ATCATTCATG CACTCCATTT GGTGCAAAGG AACCTGGAAT ATTTATATCT 
CATGTCGTGC CTGGTGGTAT AGCTGCAAAA TCTGGTAAAT TAAGAATGGG TGATAGAATA 
CTAAAGGTAA ATGGTACTGA TGTAACAAAA GCTACTCATC AAGAGGCTGT GATGGAACTT 
TTGCGACCAG GAGATCAAAT AGTGCTTACT GTCCAACACG ATCCACTACC CGAAAATTAT 
CAGCTGGTCG AAATAGAGTA CATCCCTGTG ACAGAATTAG TTATTACAAA AGAACCAGGA 
GAGAAACTGG GCATGCATAT TAAAGGTGGA CTTCGAGGAC AAAAAGGTAA TCCACTTGAT 
CATACAGATG AAGGGGTTTT TATATCCAAA ATCAACTCAG GTGGTGCAGC TAAAAGAGAT 
GGAAGACTGA AAGTTGGAAT GAGACTATTA GAAGTTAATG GTACATCATT ATTAGGCGCA 
ACTCACCAAG AAGCAGTAAA TATATTGCGA TGTTCAGGAA ATACGATTAC TTTGGTGGTT 
TGTAAAGGAT ATGATAAGAG TGAAATTGAA CCAGTATTAC CAATATCCGA TGGAAGAGAT 
TCTAAGGAAT CTAGAACATC CAAAGAATTA AAGGATGCTA CAACAGAAGG TACTAAATCG 
TTATCACAAA GTATATCCAG TCTGGATCGC GATGATGAAG AAGCAGCAAC TCTTAGACAA 
GAACAGGAAA TGAAAGCTGA ACTTGTCGCA TGGGAACAAG AAGAAAGAGA ACGTGCTTTG 
CTTGAGCATA GAGAAAAATC TACTCCTGAA AAAGTATTAG ATGTAGTAAG AGCAGCTGAA 
TCATTAGTAA GCAAATCAAA TAGTCCGGTT GATATGGTCG TACCACCAAA GTCACCAGGT 
GGTACGAAAG ATCTCAAAAC AACCACTATT GTGATGAGCA AACATACTCT AGCACCTCAA 
AATACAAATT CACTGAGGAA AGAGAGAACT GGAACATCAG TGGACAATCC ATCGCCCAAG 
TCTCCGATCT CTACTTTAAC TCCATCGATG AATTTCGATC ACACTACTGC TGTCCAAAGC 
TTGCCCTCTC CAAAGAAAAA AAGTTCCATA CCAAAACGTA GTATTACATT TGCTGATCTT 
CCTCCCATTT CTAAAGAACC AATTAATTAT TCCATTTCGG CGAAAGAAGA TCCATCACCT 
TTGCATGATA GTAAATTTAC TTTCGATCCT CAAACTATTT CTTATAAGAA AGTCTATCAA 
AATCCTATCC AAGCTACTCA TTCTCGTTTC AAACTTCCTC CTACACCTTT AACTGCTCCT 
AAATCTATAG AAGATTATGG TACTTCAATT TCTTTTGGTA AACGACAGAA CGCACGCTCT 
GTTGATGGGA ATAGTCAGGT TGAGCTTTCT CCAACAACAT CACCAACACC ACCTCTAGTA 
AAGATGTCGG TTAGTGATAA AAAAAAATTG TTTGAAAGTG CCATGGAGGA ACATTTAAAA 
CCTTCCCCTA AACCAGAAAA AGTATTCAGT TTTCTAAGTC AGGATGAAGT TGAGAAAATG 
AAACAAGAAG AAGAAAAGAA GATTGCTACT CTAACGCGGG ATGAATTAAA GTCGTGGGCA 
CAACTTGATG AAAATGAAGG TTTGGAAGAT TTAGAAGAAA CATTAGAAGA TCAGGATAAT 
CGGCGACCTA ATAGCCGACT GAGTTCGCGA ACTTCGATCC CTCTTCTGCA AAATCTTCCA 
AGCAGTGTAA GGACAGCAAA AGCAGAACGC CGCTTGAAAG AAAGATTAAT ACAGGAGGGT 
ATAATTTCGG ATGAAGATGA AGAAAGTCAT TTAAGTCCTG CCGAACAGAG AGCGTTAAGA 
GCAGAAAAAC GTGCGGCATG GAGACAAGCA CGTTTGAAAT CTCTGGAACA AGATGCTCTT 
CAAGCACAGA TAGTAATTAA AAAAATGAGT GAAATGATGG ACACGAATAA AACAAGTTCA 
ATGCAGGATT CTACAGATAT TCCTATTACT CCTGTTTCTG AATCGGAAAA GACAACTGAT 
GTTGAAACTC TACGAGAAAA GATAATATCA TTGGAATTAA GTAATGCAGA AGATGAAATA 
GAAAGTATTA CCGGTACAGG AGCAAGTGAT GCTGAAAGCG AAAGCGAAGA AATTACTGTA 
CAACCTCCTA CTATGACTAC TTTACAAGAA AGAAATGAAA CAATTATATC TACAAATGAA 
GGTGCCGCAC CTAAAATAAG TGCAAATACT GTTCTTGTTT CAAAAAAAAA GAAACGAAAA 
CGTTCGAAGA AAGGTCGTCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016921381.1
CDS	1476..7439
Translation	MFRCIPIFKGCNRQVEYIDKRHCSLPCVPDDILRYSRSLEELLLDANHIRDLPKNFFRLQKLRKLGLSDNEIHRLPPDIQNFENLVELDVSRNDIPDIPENIKNLRALQVADFSSNPIPRLPAGFVQLRNLTVLGLNDMSLTNLPPDFGSLEALQSLELRENLLKSLPESLSQLYKLERLDLGDNDIEVLPAHIGELPALQELWLDHNQLQHLPPEIGELKTLVCLDVSENRLEDLPEEIGGLEALTDLHLSQNVIEKLPDGLGELKKLTILKVDQNRLSTLNPNIGRCENLQELILTENFLLELPVSIGKLLNLNNLNVDRNSLQSLPTETGNLKQLGVLSLRDNKLQYLPIEVGQCTALHVLDVSGNRLQYLPYSLINLNLKAVWLSKNQAQPMLTFQTDVDEETGQEVLTCFLLPQLEIHPDDSGRLGMLAGMRNAVQDGEIRELGSSDDEGWQEKEASRTHSVKFTDEPPETDKETPFVRQNTPHPKELKAKAHKLFSKGKNDSRSASTDEQDVSQTFGLDKTETDVSTKPNDLTVDLIEQESLKHESVENEQKEIVPGVTDNIDVQSSNEPEIVSNQYIVESNTDVRTEGSISELKDRNDKEDDESENEETQRHVEFSIAEGTDYDGSGDSNRPNRLHRRDTPHHLKNKRIHTAVDKEKVASIIAQALMKKNDEISTSTSAPAETTENFDAQSQDAISDAEPTIEVREEQYEIHIERTTGGLGLSIAGGIGSTPFKGDDEGIFISRVTEGGPADLAGLKVEDKVLSVNGVSVVNVGHYDAVEVLKACGRVLVLVVQREVTRIVPPFEQQISSRKNSVCSSLSTSRAPSATSHVSSIGFSHNLENGDALSHDAIKCKKIPEPISSTKVGNEPMVSVLVHTTLIRDQNGLGFSIAGGEGSPPFKDNSDAIYISRITDGGVAQKDGKLLVGDKVISINGVEMRGAKHEQAVALLTGLERFVRLVVEREIPFSQANAATVVTPSEKSPRVIGAPKPYTGLYNANSYIANKPGYTGYRRSIDADRTLSPSPTSKTPPPMKIETTELPKMNGVTESGNIKNVSSISPSPTNSQLHPQPAPRHSISQPTITPTTTTSSTPTSSTEPRSTSPQEDIQVPKPITNEEFQAMIPAHFLRPPTSSPSPDSHQGPIVTVTIKQPDNLPGDVNFPPAPTTLGKVTETITKSTLTETVVTRVTENQLVPPVIIEDVILVKEGSLGFSIIGGTDHSCTPFGAKEPGIFISHVVPGGIAAKSGKLRMGDRILKVNGTDVTKATHQEAVMELLRPGDQIVLTVQHDPLPENYQLVEIEYIPVTELVITKEPGEKLGMHIKGGLRGQKGNPLDHTDEGVFISKINSGGAAKRDGRLKVGMRLLEVNGTSLLGATHQEAVNILRCSGNTITLVVCKGYDKSEIEPVLPISDGRDSKESRTSKELKDATTEGTKSLSQSISSLDRDDEEAATLRQEQEMKAELVAWEQEERERALLEHREKSTPEKVLDVVRAAESLVSKSNSPVDMVVPPKSPGGTKDLKTTTIVMSKHTLAPQNTNSLRKERTGTSVDNPSPKSPISTLTPSMNFDHTTAVQSLPSPKKKSSIPKRSITFADLPPISKEPINYSISAKEDPSPLHDSKFTFDPQTISYKKVYQNPIQATHSRFKLPPTPLTAPKSIEDYGTSISFGKRQNARSVDGNSQVELSPTTSPTPPLVKMSVSDKKKLFESAMEEHLKPSPKPEKVFSFLSQDEVEKMKQEEEKKIATLTRDELKSWAQLDENEGLEDLEETLEDQDNRRPNSRLSSRTSIPLLQNLPSSVRTAKAERRLKERLIQEGIISDEDEESHLSPAEQRALRAEKRAAWRQARLKSLEQDALQAQIVIKKMSEMMDTNKTSSMQDSTDIPITPVSESEKTTDVETLREKIISLELSNAEDEIESITGTGASDAESESEEITVQPPTMTTLQERNETIISTNEGAAPKISANTVLVSKKKKRKRSKKGRH

Target ORF information:

RefSeq Version	XM_017065892.2
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana protein lap4 (LOC108003564), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017065892.2

ORF Insert Sequence:

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGC AGATATCATC GAGAAAAAAC 
TCAGTGTGCT CTAGTTTAAG TACTAGTAGA GCTCCAAGTG CAACATCTCA TGTTTCATCT 
ATAGGTTTTT CACATAATTT AGAAAATGGT GATGCTTTAT CTCATGATGC TATTAAGTGT 
AAGAAAATTC CTGAACCTAT ATCTTCAACA AAAGTAGGCA ATGAACCAAT GGTATCAGTT 
CTTGTTCACA CAACATTAAT ACGGGACCAA AATGGGCTTG GATTTAGTAT TGCTGGTGGA 
GAAGGTTCTC CACCATTCAA AGACAATAGT GATGCGATAT ATATTTCAAG AATAACAGAT 
GGCGGCGTAG CACAAAAAGA TGGTAAATTA TTAGTTGGAG ACAAAGTAAT ATCTATTAAT 
GGAGTTGAGA TGAGAGGAGC TAAGCATGAA CAAGCAGTTG CTTTATTAAC AGGTTTAGAA 
AGATTTGTTC GATTAGTAGT TGAACGCGAA ATACCATTTT CGCAAGCAAA TGCAGCTACG 
GTCGTAACAC CTTCAGAAAA ATCACCACGA GTGATCGGTG CACCTAAACC ATATACAGGA 
TTATATAATG CCAATAGTTA TATAGCAAAT AAACCGGGAT ATACTGGATA CAGACGTTCT 
ATAGATGCTG ATAGAACATT ATCTCCAAGC CCTACTTCGA AAACGCCTCC ACCTATGAAA 
ATTGAAACTA CTGAATTACC GAAAATGAAC GGTGTTACAG AATCAGGAAA TATTAAAAAT 
GTTTCTTCGA TATCACCAAG TCCAACAAAT AGCCAACTAC ATCCACAGCC TGCTCCTAGG 
CACAGCATTT CGCAACCAAC AATTACACCT ACAACTACTA CGAGTTCAAC GCCCACATCT 
TCTACAGAAC CTAGGTCAAC CTCACCCCAG GAAGATATAC AGGTACCCAA GCCTATCACC 
AATGAAGAAT TTCAGGCTAT GATTCCTGCT CATTTCCTTC GTCCTCCTAC TTCCTCACCA 
TCGCCAGATT CCCATCAAGG CCCTATCGTG ACAGTGACAA TAAAACAGCC TGATAATCTA 
CCTGGAGATG TTAATTTTCC TCCGGCTCCT ACAACACTTG GTAAAGTTAC TGAGACCATC 
ACAAAGAGCA CTCTCACCGA GACCGTCGTA ACTAGGGTGA CTGAAAATCA ATTGGTACCA 
CCAGTAATTA TTGAGGACGT GATTCTTGTA AAAGAGGGAT CTCTAGGATT TAGTATTATT 
GGTGGTACGG ATCATTCATG CACTCCATTT GGTGCAAAGG AACCTGGAAT ATTTATATCT 
CATGTCGTGC CTGGTGGTAT AGCTGCAAAA TCTGGTAAAT TAAGAATGGG TGATAGAATA 
CTAAAGGTAA ATGGTACTGA TGTAACAAAA GCTACTCATC AAGAGGCTGT GATGGAACTT 
TTGCGACCAG GAGATCAAAT AGTGCTTACT GTCCAACACG ATCCACTACC CGAAAATTAT 
CAGCTGGTCG AAATAGAGTA CATCCCTGTG ACAGAATTAG TTATTACAAA AGAACCAGGA 
GAGAAACTGG GCATGCATAT TAAAGGTGGA CTTCGAGGAC AAAAAGGTAA TCCACTTGAT 
CATACAGATG AAGGGGTTTT TATATCCAAA ATCAACTCAG GTGGTGCAGC TAAAAGAGAT 
GGAAGACTGA AAGTTGGAAT GAGACTATTA GAAGTTAATG GTACATCATT ATTAGGCGCA 
ACTCACCAAG AAGCAGTAAA TATATTGCGA TGTTCAGGAA ATACGATTAC TTTGGTGGTT 
TGTAAAGGAT ATGATAAGAG TGAAATTGAA CCAGTATTAC CAATATCCGA TGGAAGAGAT 
TCTAAGGAAT CTAGAACATC CAAAGAATTA AAGGATGCTA CAACAGAAGG TACTAAATCG 
TTATCACAAA GTATATCCAG TCTGGATCGC GATGATGAAG AAGCAGCAAC TCTTAGACAA 
GAACAGGAAA TGAAAGCTGA ACTTGTCGCA TGGGAACAAG AAGAAAGAGA ACGTGCTTTG 
CTTGAGCATA GAGAAAAATC TACTCCTGAA AAAGTATTAG ATGTAGTAAG AGCAGCTGAA 
TCATTAGTAA GCAAATCAAA TAGTCCGGTT GATATGGTCG TACCACCAAA GTCACCAGGT 
GGTACGAAAG ATCTCAAAAC AACCACTATT GTGATGAGCA AACATACTCT AGCACCTCAA 
AATACAAATT CACTGAGGAA AGAGAGAACT GGAACATCAG TGGACAATCC ATCGCCCAAG 
TCTCCGATCT CTACTTTAAC TCCATCGATG AATTTCGATC ACACTACTGC TGTCCAAAGC 
TTGCCCTCTC CAAAGAAAAA AAGTTCCATA CCAAAACGTA GTATTACATT TGCTGATCTT 
CCTCCCATTT CTAAAGAACC AATTAATTAT TCCATTTCGG CGAAAGAAGA TCCATCACCT 
TTGCATGATA GTAAATTTAC TTTCGATCCT CAAACTATTT CTTATAAGAA AGTCTATCAA 
AATCCTATCC AAGCTACTCA TTCTCGTTTC AAACTTCCTC CTACACCTTT AACTGCTCCT 
AAATCTATAG AAGATTATGG TACTTCAATT TCTTTTGGTA AACGACAGAA CGCACGCTCT 
GTTGATGGGA ATAGTCAGGT TGAGCTTTCT CCAACAACAT CACCAACACC ACCTCTAGTA 
AAGATGTCGG TTAGTGATAA AAAAAAATTG TTTGAAAGTG CCATGGAGGA ACATTTAAAA 
CCTTCCCCTA AACCAGAAAA AGTATTCAGT TTTCTAAGTC AGGATGAAGT TGAGAAAATG 
AAACAAGAAG AAGAAAAGAA GATTGCTACT CTAACGCGGG ATGAATTAAA GTCGTGGGCA 
CAACTTGATG AAAATGAAGG TTTGGAAGAT TTAGAAGAAA CATTAGAAGA TCAGGATAAT 
CGGCGACCTA ATAGCCGACT GAGTTCGCGA ACTTCGATCC CTCTTCTGCA AAATCTTCCA 
AGCAGTGTAA GGACAGCAAA AGCAGAACGC CGCTTGAAAG AAAGATTAAT ACAGGAGGGT 
ATAATTTCGG ATGAAGATGA AGAAAGTCAT TTAAGTCCTG CCGAACAGAG AGCGTTAAGA 
GCAGAAAAAC GTGCGGCATG GAGACAAGCA CGTTTGAAAT CTCTGGAACA AGATGCTCTT 
CAAGCACAGA TAGTAATTAA AAAAATGAGT GAAATGATGG ACACGAATAA AACAAGTTCA 
ATGCAGGATT CTACAGATAT TCCTATTACT CCTGTTTCTG AATCGGAAAA GACAACTGAT 
GTTGAAACTC TACGAGAAAA GATAATATCA TTGGAATTAA GTAATGCAGA AGATGAAATA 
GAAAGTATTA CCGGTACAGG AGCAAGTGAT GCTGAAAGCG AAAGCGAAGA AATTACTGTA 
CAACCTCCTA CTATGACTAC TTTACAAGAA AGAAATGAAA CAATTATATC TACAAATGAA 
GGTGCCGCAC CTAAAATAAG TGCAAATACT GTTCTTGTTT CAAAAAAAAA GAAACGAAAA 
CGTTCGAAGA AAGGTCGTCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108003564 ( XM_017065894.1 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_016921383.1 Apis cerana protein lap4 (LOC108003564), transcript variant X8, mRNA.

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CloneID	OAp29727
Clone ID Related Accession (Same CDS sequence)	XM_017065894.1 , XM_017065894.2
Accession Version	XM_017065894.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-08
Organism	Apis cerana(Asiatic honeybee)
Product	protein lap4 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016017567.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis cerana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGC AGATATCATC GAGAAAAAAC 
TCAGTGTGCT CTAGTTTAAG TACTAGTAGA GCTCCAAGTG CAACATCTCA TGTTTCATCT 
ATAGGTTTTT CACATAATTT AGAAAATGGT GATGCTTTAT CTCATGATGC TATTAAGTGT 
AAGAAAATTC CTGAACCTAT ATCTTCAACA AAAGTAGGCA ATGAACCAAT GGTATCAGTT 
CTTGTTCACA CAACATTAAT ACGGGACCAA AATGGGCTTG GATTTAGTAT TGCTGGTGGA 
GAAGGTTCTC CACCATTCAA AGACAATAGT GATGCGATAT ATATTTCAAG AATAACAGAT 
GGCGGCGTAG CACAAAAAGA TGGTAAATTA TTAGTTGGAG ACAAAGTAAT ATCTATTAAT 
GGAGTTGAGA TGAGAGGAGC TAAGCATGAA CAAGCAGTTG CTTTATTAAC AGGTTTAGAA 
AGATTTGTTC GATTAGTAGT TGAACGCGAA ATACCATTTT CGCAAGCAAA TGCAGCTACG 
GTCGTAACAC CTTCAGAAAA ATCACCACGA GTGATCGGTG CACCTAAACC ATATACAGGA 
TTATATAATG CCAATAGTTA TATAGCAAAT AAACCGGGAT ATACTGGATA CAGACGTTCT 
ATAGATGCTG ATAGAACATT ATCTCCAAGC CCTACTTCGA AAACGCCTCC ACCTATGAAA 
ATTGAAACTA CTGAATTACC GAAAATGAAC GGTGTTACAG AATCAGGAAA TATTAAAAAT 
GTTTCTTCGA TATCACCAAG TCCAACAAAT AGCCAACTAC ATCCACAGCC TGCTCCTAGG 
CACAGCATTT CGCAACCAAC AATTACACCT ACAACTACTA CGAGTTCAAC GCCCACATCT 
TCTACAGAAC CTAGGTCAAC CTCACCCCAG GAAGATATAC AGGACGTGAT TCTTGTAAAA 
GAGGGATCTC TAGGATTTAG TATTATTGGT GGTACGGATC ATTCATGCAC TCCATTTGGT 
GCAAAGGAAC CTGGAATATT TATATCTCAT GTCGTGCCTG GTGGTATAGC TGCAAAATCT 
GGTAAATTAA GAATGGGTGA TAGAATACTA AAGGTAAATG GTACTGATGT AACAAAAGCT 
ACTCATCAAG AGGCTGTGAT GGAACTTTTG CGACCAGGAG ATCAAATAGT GCTTACTGTC 
CAACACGATC CACTACCCGA AAATTATCAG CTGGTCGAAA TAGAGTACAT CCCTGTGACA 
GAATTAGTTA TTACAAAAGA ACCAGGAGAG AAACTGGGCA TGCATATTAA AGGTGGACTT 
CGAGGACAAA AAGGTAATCC ACTTGATCAT ACAGATGAAG GGGTTTTTAT ATCCAAAATC 
AACTCAGGTG GTGCAGCTAA AAGAGATGGA AGACTGAAAG TTGGAATGAG ACTATTAGAA 
GTTAATGGTA CATCATTATT AGGCGCAACT CACCAAGAAG CAGTAAATAT ATTGCGATGT 
TCAGGAAATA CGATTACTTT GGTGGTTTGT AAAGGATATG ATAAGAGTGA AATTGAACCA 
GTATTACCAA TATCCGATGG AAGAGATTCT AAGGAATCTA GAACATCCAA AGAATTAAAG 
GATGCTACAA CAGAAGGTAC TAAATCGTTA TCACAAAGTA TATCCAGTCT GGATCGCGAT 
GATGAAGAAG CAGCAACTCT TAGACAAGAA CAGGAAATGA AAGCTGAACT TGTCGCATGG 
GAACAAGAAG AAAGAGAACG TGCTTTGCTT GAGCATAGAG AAAAATCTAC TCCTGAAAAA 
GTATTAGATG TAGTAAGAGC AGCTGAATCA TTAGTAAGCA AATCAAATAG TCCGGTTGAT 
ATGGTCGTAC CACCAAAGTC ACCAGGTGGT ACGAAAGATC TCAAAACAAC CACTATTGTG 
ATGAGCAAAC ATACTCTAGC ACCTCAAAAT ACAAATTCAC TGAGGAAAGA GAGAACTGGA 
ACATCAGTGG ACAATCCATC GCCCAAGTCT CCGATCTCTA CTTTAACTCC ATCGATGAAT 
TTCGATCACA CTACTGCTGT CCAAAGCTTG CCCTCTCCAA AGAAAAAAAG TTCCATACCA 
AAACGTAGTA TTACATTTGC TGATCTTCCT CCCATTTCTA AAGAACCAAT TAATTATTCC 
ATTTCGGCGA AAGAAGATCC ATCACCTTTG CATGATAGTA AATTTACTTT CGATCCTCAA 
ACTATTTCTT ATAAGAAAGT CTATCAAAAT CCTATCCAAG CTACTCATTC TCGTTTCAAA 
CTTCCTCCTA CACCTTTAAC TGCTCCTAAA TCTATAGAAG ATTATGGTAC TTCAATTTCT 
TTTGGTAAAC GACAGAACGC ACGCTCTGTT GATGGGAATA GTCAGGTTGA GCTTTCTCCA 
ACAACATCAC CAACACCACC TCTAGTAAAG ATGTCGGTTA GTGATAAAAA AAAATTGTTT 
GAAAGTGCCA TGGAGGAACA TTTAAAACCT TCCCCTAAAC CAGAAAAAGT ATTCAGTTTT 
CTAAGTCAGG ATGAAGTTGA GAAAATGAAA CAAGAAGAAG AAAAGAAGAT TGCTACTCTA 
ACGCGGGATG AATTAAAGTC GTGGGCACAA CTTGATGAAA ATGAAGGTTT GGAAGATTTA 
GAAGAAACAT TAGAAGATCA GGATAATCGG CGACCTAATA GCCGACTGAG TTCGCGAACT 
TCGATCCCTC TTCTGCAAAA TCTTCCAAGC AGTGTAAGGA CAGCAAAAGC AGAACGCCGC 
TTGAAAGAAA GATTAATACA GGAGGGTATA ATTTCGGATG AAGATGAAGA AAGTCATTTA 
AGTCCTGCCG AACAGAGAGC GTTAAGAGCA GAAAAACGTG CGGCATGGAG ACAAGCACGT 
TTGAAATCTC TGGAACAAGA TGCTCTTCAA GCACAGATAG TAATTAAAAA AATGAGTGAA 
ATGATGGACA CGAATAAAAC AAGTTCAATG CAGGATTCTA CAGATATTCC TATTACTCCT 
GTTTCTGAAT CGGAAAAGAC AACTGATTTC ACTACGTTAC GGCCGTCTAG TGCAGATTTT 
CCCAAACTTG CTGTACGTAG CAAAGTGGGT CCCCCTAAAG AGATACGCGA ATCCGAGAAA 
GTAGTGGACG AGAAGGTCAC TCGACGTACT GAGGAATATG TGGATGAGGT GACGGGTGAA 
CGACGTGTGC GAACAGTCGA ATACGTCGAG AAACTCATTG AACGACAGGT TGAAACTCTA 
CGAGAAAAGA TAATATCATT GGAATTAAGT AATGCAGAAG ATGAAATAGA AAGTATTACC 
GGTACAGGAG CAAGTGATGC TGAAAGCGAA AGCGAAGAAA TTACTGTACA ACCTCCTACT 
ATGACTACTT TACAAGAAAG AAATGAAACA ATTATATCTA CAAATGAAGG TGCCGCACCT 
AAAATAAGTG CAAATACTGT TCTTGTTTCA AAAAAAAAGA AACGAAAACG TTCGAAGAAA 
GGTCGTCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016921383.1
CDS	233..6124
Translation	MFRCIPIFKGCNRQVEYIDKRHCSLPCVPDDILRYSRSLEELLLDANHIRDLPKNFFRLQKLRKLGLSDNEIHRLPPDIQNFENLVELDVSRNDIPDIPENIKNLRALQVADFSSNPIPRLPAGFVQLRNLTVLGLNDMSLTNLPPDFGSLEALQSLELRENLLKSLPESLSQLYKLERLDLGDNDIEVLPAHIGELPALQELWLDHNQLQHLPPEIGELKTLVCLDVSENRLEDLPEEIGGLEALTDLHLSQNVIEKLPDGLGELKKLTILKVDQNRLSTLNPNIGRCENLQELILTENFLLELPVSIGKLLNLNNLNVDRNSLQSLPTETGNLKQLGVLSLRDNKLQYLPIEVGQCTALHVLDVSGNRLQYLPYSLINLNLKAVWLSKNQAQPMLTFQTDVDEETGQEVLTCFLLPQLEIHPDDSGRLGMLAGMRNAVQDGEIRELGSSDDEGWQEKEASRTHSVKFTDEPPETDKETPFVRQNTPHPKELKAKAHKLFSKGKNDSRSASTDEQDVSQTFGLDKTETDVSTKPNDLTVDLIEQESLKHESVENEQKEIVPGVTDNIDVQSSNEPEIVSNQYIVESNTDVRTEGSISELKDRNDKEDDESENEETQRHVEFSIAEGTDYDGSGDSNRPNRLHRRDTPHHLKNKRIHTAVDKEKVASIIAQALMKKNDEISTSTSAPAETTENFDAQSQDAISDAEPTIEVREEQYEIHIERTTGGLGLSIAGGIGSTPFKGDDEGIFISRVTEGGPADLAGLKVEDKVLSVNGVSVVNVGHYDAVEVLKACGRVLVLVVQREVTRIVPPFEQQISSRKNSVCSSLSTSRAPSATSHVSSIGFSHNLENGDALSHDAIKCKKIPEPISSTKVGNEPMVSVLVHTTLIRDQNGLGFSIAGGEGSPPFKDNSDAIYISRITDGGVAQKDGKLLVGDKVISINGVEMRGAKHEQAVALLTGLERFVRLVVEREIPFSQANAATVVTPSEKSPRVIGAPKPYTGLYNANSYIANKPGYTGYRRSIDADRTLSPSPTSKTPPPMKIETTELPKMNGVTESGNIKNVSSISPSPTNSQLHPQPAPRHSISQPTITPTTTTSSTPTSSTEPRSTSPQEDIQDVILVKEGSLGFSIIGGTDHSCTPFGAKEPGIFISHVVPGGIAAKSGKLRMGDRILKVNGTDVTKATHQEAVMELLRPGDQIVLTVQHDPLPENYQLVEIEYIPVTELVITKEPGEKLGMHIKGGLRGQKGNPLDHTDEGVFISKINSGGAAKRDGRLKVGMRLLEVNGTSLLGATHQEAVNILRCSGNTITLVVCKGYDKSEIEPVLPISDGRDSKESRTSKELKDATTEGTKSLSQSISSLDRDDEEAATLRQEQEMKAELVAWEQEERERALLEHREKSTPEKVLDVVRAAESLVSKSNSPVDMVVPPKSPGGTKDLKTTTIVMSKHTLAPQNTNSLRKERTGTSVDNPSPKSPISTLTPSMNFDHTTAVQSLPSPKKKSSIPKRSITFADLPPISKEPINYSISAKEDPSPLHDSKFTFDPQTISYKKVYQNPIQATHSRFKLPPTPLTAPKSIEDYGTSISFGKRQNARSVDGNSQVELSPTTSPTPPLVKMSVSDKKKLFESAMEEHLKPSPKPEKVFSFLSQDEVEKMKQEEEKKIATLTRDELKSWAQLDENEGLEDLEETLEDQDNRRPNSRLSSRTSIPLLQNLPSSVRTAKAERRLKERLIQEGIISDEDEESHLSPAEQRALRAEKRAAWRQARLKSLEQDALQAQIVIKKMSEMMDTNKTSSMQDSTDIPITPVSESEKTTDFTTLRPSSADFPKLAVRSKVGPPKEIRESEKVVDEKVTRRTEEYVDEVTGERRVRTVEYVEKLIERQVETLREKIISLELSNAEDEIESITGTGASDAESESEEITVQPPTMTTLQERNETIISTNEGAAPKISANTVLVSKKKKRKRSKKGRH

Target ORF information:

RefSeq Version	XM_017065894.1
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana protein lap4 (LOC108003564), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017065894.1

ORF Insert Sequence:

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGC AGATATCATC GAGAAAAAAC 
TCAGTGTGCT CTAGTTTAAG TACTAGTAGA GCTCCAAGTG CAACATCTCA TGTTTCATCT 
ATAGGTTTTT CACATAATTT AGAAAATGGT GATGCTTTAT CTCATGATGC TATTAAGTGT 
AAGAAAATTC CTGAACCTAT ATCTTCAACA AAAGTAGGCA ATGAACCAAT GGTATCAGTT 
CTTGTTCACA CAACATTAAT ACGGGACCAA AATGGGCTTG GATTTAGTAT TGCTGGTGGA 
GAAGGTTCTC CACCATTCAA AGACAATAGT GATGCGATAT ATATTTCAAG AATAACAGAT 
GGCGGCGTAG CACAAAAAGA TGGTAAATTA TTAGTTGGAG ACAAAGTAAT ATCTATTAAT 
GGAGTTGAGA TGAGAGGAGC TAAGCATGAA CAAGCAGTTG CTTTATTAAC AGGTTTAGAA 
AGATTTGTTC GATTAGTAGT TGAACGCGAA ATACCATTTT CGCAAGCAAA TGCAGCTACG 
GTCGTAACAC CTTCAGAAAA ATCACCACGA GTGATCGGTG CACCTAAACC ATATACAGGA 
TTATATAATG CCAATAGTTA TATAGCAAAT AAACCGGGAT ATACTGGATA CAGACGTTCT 
ATAGATGCTG ATAGAACATT ATCTCCAAGC CCTACTTCGA AAACGCCTCC ACCTATGAAA 
ATTGAAACTA CTGAATTACC GAAAATGAAC GGTGTTACAG AATCAGGAAA TATTAAAAAT 
GTTTCTTCGA TATCACCAAG TCCAACAAAT AGCCAACTAC ATCCACAGCC TGCTCCTAGG 
CACAGCATTT CGCAACCAAC AATTACACCT ACAACTACTA CGAGTTCAAC GCCCACATCT 
TCTACAGAAC CTAGGTCAAC CTCACCCCAG GAAGATATAC AGGACGTGAT TCTTGTAAAA 
GAGGGATCTC TAGGATTTAG TATTATTGGT GGTACGGATC ATTCATGCAC TCCATTTGGT 
GCAAAGGAAC CTGGAATATT TATATCTCAT GTCGTGCCTG GTGGTATAGC TGCAAAATCT 
GGTAAATTAA GAATGGGTGA TAGAATACTA AAGGTAAATG GTACTGATGT AACAAAAGCT 
ACTCATCAAG AGGCTGTGAT GGAACTTTTG CGACCAGGAG ATCAAATAGT GCTTACTGTC 
CAACACGATC CACTACCCGA AAATTATCAG CTGGTCGAAA TAGAGTACAT CCCTGTGACA 
GAATTAGTTA TTACAAAAGA ACCAGGAGAG AAACTGGGCA TGCATATTAA AGGTGGACTT 
CGAGGACAAA AAGGTAATCC ACTTGATCAT ACAGATGAAG GGGTTTTTAT ATCCAAAATC 
AACTCAGGTG GTGCAGCTAA AAGAGATGGA AGACTGAAAG TTGGAATGAG ACTATTAGAA 
GTTAATGGTA CATCATTATT AGGCGCAACT CACCAAGAAG CAGTAAATAT ATTGCGATGT 
TCAGGAAATA CGATTACTTT GGTGGTTTGT AAAGGATATG ATAAGAGTGA AATTGAACCA 
GTATTACCAA TATCCGATGG AAGAGATTCT AAGGAATCTA GAACATCCAA AGAATTAAAG 
GATGCTACAA CAGAAGGTAC TAAATCGTTA TCACAAAGTA TATCCAGTCT GGATCGCGAT 
GATGAAGAAG CAGCAACTCT TAGACAAGAA CAGGAAATGA AAGCTGAACT TGTCGCATGG 
GAACAAGAAG AAAGAGAACG TGCTTTGCTT GAGCATAGAG AAAAATCTAC TCCTGAAAAA 
GTATTAGATG TAGTAAGAGC AGCTGAATCA TTAGTAAGCA AATCAAATAG TCCGGTTGAT 
ATGGTCGTAC CACCAAAGTC ACCAGGTGGT ACGAAAGATC TCAAAACAAC CACTATTGTG 
ATGAGCAAAC ATACTCTAGC ACCTCAAAAT ACAAATTCAC TGAGGAAAGA GAGAACTGGA 
ACATCAGTGG ACAATCCATC GCCCAAGTCT CCGATCTCTA CTTTAACTCC ATCGATGAAT 
TTCGATCACA CTACTGCTGT CCAAAGCTTG CCCTCTCCAA AGAAAAAAAG TTCCATACCA 
AAACGTAGTA TTACATTTGC TGATCTTCCT CCCATTTCTA AAGAACCAAT TAATTATTCC 
ATTTCGGCGA AAGAAGATCC ATCACCTTTG CATGATAGTA AATTTACTTT CGATCCTCAA 
ACTATTTCTT ATAAGAAAGT CTATCAAAAT CCTATCCAAG CTACTCATTC TCGTTTCAAA 
CTTCCTCCTA CACCTTTAAC TGCTCCTAAA TCTATAGAAG ATTATGGTAC TTCAATTTCT 
TTTGGTAAAC GACAGAACGC ACGCTCTGTT GATGGGAATA GTCAGGTTGA GCTTTCTCCA 
ACAACATCAC CAACACCACC TCTAGTAAAG ATGTCGGTTA GTGATAAAAA AAAATTGTTT 
GAAAGTGCCA TGGAGGAACA TTTAAAACCT TCCCCTAAAC CAGAAAAAGT ATTCAGTTTT 
CTAAGTCAGG ATGAAGTTGA GAAAATGAAA CAAGAAGAAG AAAAGAAGAT TGCTACTCTA 
ACGCGGGATG AATTAAAGTC GTGGGCACAA CTTGATGAAA ATGAAGGTTT GGAAGATTTA 
GAAGAAACAT TAGAAGATCA GGATAATCGG CGACCTAATA GCCGACTGAG TTCGCGAACT 
TCGATCCCTC TTCTGCAAAA TCTTCCAAGC AGTGTAAGGA CAGCAAAAGC AGAACGCCGC 
TTGAAAGAAA GATTAATACA GGAGGGTATA ATTTCGGATG AAGATGAAGA AAGTCATTTA 
AGTCCTGCCG AACAGAGAGC GTTAAGAGCA GAAAAACGTG CGGCATGGAG ACAAGCACGT 
TTGAAATCTC TGGAACAAGA TGCTCTTCAA GCACAGATAG TAATTAAAAA AATGAGTGAA 
ATGATGGACA CGAATAAAAC AAGTTCAATG CAGGATTCTA CAGATATTCC TATTACTCCT 
GTTTCTGAAT CGGAAAAGAC AACTGATTTC ACTACGTTAC GGCCGTCTAG TGCAGATTTT 
CCCAAACTTG CTGTACGTAG CAAAGTGGGT CCCCCTAAAG AGATACGCGA ATCCGAGAAA 
GTAGTGGACG AGAAGGTCAC TCGACGTACT GAGGAATATG TGGATGAGGT GACGGGTGAA 
CGACGTGTGC GAACAGTCGA ATACGTCGAG AAACTCATTG AACGACAGGT TGAAACTCTA 
CGAGAAAAGA TAATATCATT GGAATTAAGT AATGCAGAAG ATGAAATAGA AAGTATTACC 
GGTACAGGAG CAAGTGATGC TGAAAGCGAA AGCGAAGAAA TTACTGTACA ACCTCCTACT 
ATGACTACTT TACAAGAAAG AAATGAAACA ATTATATCTA CAAATGAAGG TGCCGCACCT 
AAAATAAGTG CAAATACTGT TCTTGTTTCA AAAAAAAAGA AACGAAAACG TTCGAAGAAA 
GGTCGTCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108003564 ( XM_017065894.2 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_016921383.1 Apis cerana protein lap4 (LOC108003564), transcript variant X8, mRNA.

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CloneID	OAp29727
Clone ID Related Accession (Same CDS sequence)	XM_017065894.1 , XM_017065894.2
Accession Version	XM_017065894.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-16
Organism	Apis cerana(Asiatic honeybee)
Product	protein lap4 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016017567.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 17, 2019 this sequence version replaced XM_017065894.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis cerana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGC AGATATCATC GAGAAAAAAC 
TCAGTGTGCT CTAGTTTAAG TACTAGTAGA GCTCCAAGTG CAACATCTCA TGTTTCATCT 
ATAGGTTTTT CACATAATTT AGAAAATGGT GATGCTTTAT CTCATGATGC TATTAAGTGT 
AAGAAAATTC CTGAACCTAT ATCTTCAACA AAAGTAGGCA ATGAACCAAT GGTATCAGTT 
CTTGTTCACA CAACATTAAT ACGGGACCAA AATGGGCTTG GATTTAGTAT TGCTGGTGGA 
GAAGGTTCTC CACCATTCAA AGACAATAGT GATGCGATAT ATATTTCAAG AATAACAGAT 
GGCGGCGTAG CACAAAAAGA TGGTAAATTA TTAGTTGGAG ACAAAGTAAT ATCTATTAAT 
GGAGTTGAGA TGAGAGGAGC TAAGCATGAA CAAGCAGTTG CTTTATTAAC AGGTTTAGAA 
AGATTTGTTC GATTAGTAGT TGAACGCGAA ATACCATTTT CGCAAGCAAA TGCAGCTACG 
GTCGTAACAC CTTCAGAAAA ATCACCACGA GTGATCGGTG CACCTAAACC ATATACAGGA 
TTATATAATG CCAATAGTTA TATAGCAAAT AAACCGGGAT ATACTGGATA CAGACGTTCT 
ATAGATGCTG ATAGAACATT ATCTCCAAGC CCTACTTCGA AAACGCCTCC ACCTATGAAA 
ATTGAAACTA CTGAATTACC GAAAATGAAC GGTGTTACAG AATCAGGAAA TATTAAAAAT 
GTTTCTTCGA TATCACCAAG TCCAACAAAT AGCCAACTAC ATCCACAGCC TGCTCCTAGG 
CACAGCATTT CGCAACCAAC AATTACACCT ACAACTACTA CGAGTTCAAC GCCCACATCT 
TCTACAGAAC CTAGGTCAAC CTCACCCCAG GAAGATATAC AGGACGTGAT TCTTGTAAAA 
GAGGGATCTC TAGGATTTAG TATTATTGGT GGTACGGATC ATTCATGCAC TCCATTTGGT 
GCAAAGGAAC CTGGAATATT TATATCTCAT GTCGTGCCTG GTGGTATAGC TGCAAAATCT 
GGTAAATTAA GAATGGGTGA TAGAATACTA AAGGTAAATG GTACTGATGT AACAAAAGCT 
ACTCATCAAG AGGCTGTGAT GGAACTTTTG CGACCAGGAG ATCAAATAGT GCTTACTGTC 
CAACACGATC CACTACCCGA AAATTATCAG CTGGTCGAAA TAGAGTACAT CCCTGTGACA 
GAATTAGTTA TTACAAAAGA ACCAGGAGAG AAACTGGGCA TGCATATTAA AGGTGGACTT 
CGAGGACAAA AAGGTAATCC ACTTGATCAT ACAGATGAAG GGGTTTTTAT ATCCAAAATC 
AACTCAGGTG GTGCAGCTAA AAGAGATGGA AGACTGAAAG TTGGAATGAG ACTATTAGAA 
GTTAATGGTA CATCATTATT AGGCGCAACT CACCAAGAAG CAGTAAATAT ATTGCGATGT 
TCAGGAAATA CGATTACTTT GGTGGTTTGT AAAGGATATG ATAAGAGTGA AATTGAACCA 
GTATTACCAA TATCCGATGG AAGAGATTCT AAGGAATCTA GAACATCCAA AGAATTAAAG 
GATGCTACAA CAGAAGGTAC TAAATCGTTA TCACAAAGTA TATCCAGTCT GGATCGCGAT 
GATGAAGAAG CAGCAACTCT TAGACAAGAA CAGGAAATGA AAGCTGAACT TGTCGCATGG 
GAACAAGAAG AAAGAGAACG TGCTTTGCTT GAGCATAGAG AAAAATCTAC TCCTGAAAAA 
GTATTAGATG TAGTAAGAGC AGCTGAATCA TTAGTAAGCA AATCAAATAG TCCGGTTGAT 
ATGGTCGTAC CACCAAAGTC ACCAGGTGGT ACGAAAGATC TCAAAACAAC CACTATTGTG 
ATGAGCAAAC ATACTCTAGC ACCTCAAAAT ACAAATTCAC TGAGGAAAGA GAGAACTGGA 
ACATCAGTGG ACAATCCATC GCCCAAGTCT CCGATCTCTA CTTTAACTCC ATCGATGAAT 
TTCGATCACA CTACTGCTGT CCAAAGCTTG CCCTCTCCAA AGAAAAAAAG TTCCATACCA 
AAACGTAGTA TTACATTTGC TGATCTTCCT CCCATTTCTA AAGAACCAAT TAATTATTCC 
ATTTCGGCGA AAGAAGATCC ATCACCTTTG CATGATAGTA AATTTACTTT CGATCCTCAA 
ACTATTTCTT ATAAGAAAGT CTATCAAAAT CCTATCCAAG CTACTCATTC TCGTTTCAAA 
CTTCCTCCTA CACCTTTAAC TGCTCCTAAA TCTATAGAAG ATTATGGTAC TTCAATTTCT 
TTTGGTAAAC GACAGAACGC ACGCTCTGTT GATGGGAATA GTCAGGTTGA GCTTTCTCCA 
ACAACATCAC CAACACCACC TCTAGTAAAG ATGTCGGTTA GTGATAAAAA AAAATTGTTT 
GAAAGTGCCA TGGAGGAACA TTTAAAACCT TCCCCTAAAC CAGAAAAAGT ATTCAGTTTT 
CTAAGTCAGG ATGAAGTTGA GAAAATGAAA CAAGAAGAAG AAAAGAAGAT TGCTACTCTA 
ACGCGGGATG AATTAAAGTC GTGGGCACAA CTTGATGAAA ATGAAGGTTT GGAAGATTTA 
GAAGAAACAT TAGAAGATCA GGATAATCGG CGACCTAATA GCCGACTGAG TTCGCGAACT 
TCGATCCCTC TTCTGCAAAA TCTTCCAAGC AGTGTAAGGA CAGCAAAAGC AGAACGCCGC 
TTGAAAGAAA GATTAATACA GGAGGGTATA ATTTCGGATG AAGATGAAGA AAGTCATTTA 
AGTCCTGCCG AACAGAGAGC GTTAAGAGCA GAAAAACGTG CGGCATGGAG ACAAGCACGT 
TTGAAATCTC TGGAACAAGA TGCTCTTCAA GCACAGATAG TAATTAAAAA AATGAGTGAA 
ATGATGGACA CGAATAAAAC AAGTTCAATG CAGGATTCTA CAGATATTCC TATTACTCCT 
GTTTCTGAAT CGGAAAAGAC AACTGATTTC ACTACGTTAC GGCCGTCTAG TGCAGATTTT 
CCCAAACTTG CTGTACGTAG CAAAGTGGGT CCCCCTAAAG AGATACGCGA ATCCGAGAAA 
GTAGTGGACG AGAAGGTCAC TCGACGTACT GAGGAATATG TGGATGAGGT GACGGGTGAA 
CGACGTGTGC GAACAGTCGA ATACGTCGAG AAACTCATTG AACGACAGGT TGAAACTCTA 
CGAGAAAAGA TAATATCATT GGAATTAAGT AATGCAGAAG ATGAAATAGA AAGTATTACC 
GGTACAGGAG CAAGTGATGC TGAAAGCGAA AGCGAAGAAA TTACTGTACA ACCTCCTACT 
ATGACTACTT TACAAGAAAG AAATGAAACA ATTATATCTA CAAATGAAGG TGCCGCACCT 
AAAATAAGTG CAAATACTGT TCTTGTTTCA AAAAAAAAGA AACGAAAACG TTCGAAGAAA 
GGTCGTCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016921383.1
CDS	1476..7367
Translation	MFRCIPIFKGCNRQVEYIDKRHCSLPCVPDDILRYSRSLEELLLDANHIRDLPKNFFRLQKLRKLGLSDNEIHRLPPDIQNFENLVELDVSRNDIPDIPENIKNLRALQVADFSSNPIPRLPAGFVQLRNLTVLGLNDMSLTNLPPDFGSLEALQSLELRENLLKSLPESLSQLYKLERLDLGDNDIEVLPAHIGELPALQELWLDHNQLQHLPPEIGELKTLVCLDVSENRLEDLPEEIGGLEALTDLHLSQNVIEKLPDGLGELKKLTILKVDQNRLSTLNPNIGRCENLQELILTENFLLELPVSIGKLLNLNNLNVDRNSLQSLPTETGNLKQLGVLSLRDNKLQYLPIEVGQCTALHVLDVSGNRLQYLPYSLINLNLKAVWLSKNQAQPMLTFQTDVDEETGQEVLTCFLLPQLEIHPDDSGRLGMLAGMRNAVQDGEIRELGSSDDEGWQEKEASRTHSVKFTDEPPETDKETPFVRQNTPHPKELKAKAHKLFSKGKNDSRSASTDEQDVSQTFGLDKTETDVSTKPNDLTVDLIEQESLKHESVENEQKEIVPGVTDNIDVQSSNEPEIVSNQYIVESNTDVRTEGSISELKDRNDKEDDESENEETQRHVEFSIAEGTDYDGSGDSNRPNRLHRRDTPHHLKNKRIHTAVDKEKVASIIAQALMKKNDEISTSTSAPAETTENFDAQSQDAISDAEPTIEVREEQYEIHIERTTGGLGLSIAGGIGSTPFKGDDEGIFISRVTEGGPADLAGLKVEDKVLSVNGVSVVNVGHYDAVEVLKACGRVLVLVVQREVTRIVPPFEQQISSRKNSVCSSLSTSRAPSATSHVSSIGFSHNLENGDALSHDAIKCKKIPEPISSTKVGNEPMVSVLVHTTLIRDQNGLGFSIAGGEGSPPFKDNSDAIYISRITDGGVAQKDGKLLVGDKVISINGVEMRGAKHEQAVALLTGLERFVRLVVEREIPFSQANAATVVTPSEKSPRVIGAPKPYTGLYNANSYIANKPGYTGYRRSIDADRTLSPSPTSKTPPPMKIETTELPKMNGVTESGNIKNVSSISPSPTNSQLHPQPAPRHSISQPTITPTTTTSSTPTSSTEPRSTSPQEDIQDVILVKEGSLGFSIIGGTDHSCTPFGAKEPGIFISHVVPGGIAAKSGKLRMGDRILKVNGTDVTKATHQEAVMELLRPGDQIVLTVQHDPLPENYQLVEIEYIPVTELVITKEPGEKLGMHIKGGLRGQKGNPLDHTDEGVFISKINSGGAAKRDGRLKVGMRLLEVNGTSLLGATHQEAVNILRCSGNTITLVVCKGYDKSEIEPVLPISDGRDSKESRTSKELKDATTEGTKSLSQSISSLDRDDEEAATLRQEQEMKAELVAWEQEERERALLEHREKSTPEKVLDVVRAAESLVSKSNSPVDMVVPPKSPGGTKDLKTTTIVMSKHTLAPQNTNSLRKERTGTSVDNPSPKSPISTLTPSMNFDHTTAVQSLPSPKKKSSIPKRSITFADLPPISKEPINYSISAKEDPSPLHDSKFTFDPQTISYKKVYQNPIQATHSRFKLPPTPLTAPKSIEDYGTSISFGKRQNARSVDGNSQVELSPTTSPTPPLVKMSVSDKKKLFESAMEEHLKPSPKPEKVFSFLSQDEVEKMKQEEEKKIATLTRDELKSWAQLDENEGLEDLEETLEDQDNRRPNSRLSSRTSIPLLQNLPSSVRTAKAERRLKERLIQEGIISDEDEESHLSPAEQRALRAEKRAAWRQARLKSLEQDALQAQIVIKKMSEMMDTNKTSSMQDSTDIPITPVSESEKTTDFTTLRPSSADFPKLAVRSKVGPPKEIRESEKVVDEKVTRRTEEYVDEVTGERRVRTVEYVEKLIERQVETLREKIISLELSNAEDEIESITGTGASDAESESEEITVQPPTMTTLQERNETIISTNEGAAPKISANTVLVSKKKKRKRSKKGRH

Target ORF information:

RefSeq Version	XM_017065894.2
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana protein lap4 (LOC108003564), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017065894.2

ORF Insert Sequence:

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGC AGATATCATC GAGAAAAAAC 
TCAGTGTGCT CTAGTTTAAG TACTAGTAGA GCTCCAAGTG CAACATCTCA TGTTTCATCT 
ATAGGTTTTT CACATAATTT AGAAAATGGT GATGCTTTAT CTCATGATGC TATTAAGTGT 
AAGAAAATTC CTGAACCTAT ATCTTCAACA AAAGTAGGCA ATGAACCAAT GGTATCAGTT 
CTTGTTCACA CAACATTAAT ACGGGACCAA AATGGGCTTG GATTTAGTAT TGCTGGTGGA 
GAAGGTTCTC CACCATTCAA AGACAATAGT GATGCGATAT ATATTTCAAG AATAACAGAT 
GGCGGCGTAG CACAAAAAGA TGGTAAATTA TTAGTTGGAG ACAAAGTAAT ATCTATTAAT 
GGAGTTGAGA TGAGAGGAGC TAAGCATGAA CAAGCAGTTG CTTTATTAAC AGGTTTAGAA 
AGATTTGTTC GATTAGTAGT TGAACGCGAA ATACCATTTT CGCAAGCAAA TGCAGCTACG 
GTCGTAACAC CTTCAGAAAA ATCACCACGA GTGATCGGTG CACCTAAACC ATATACAGGA 
TTATATAATG CCAATAGTTA TATAGCAAAT AAACCGGGAT ATACTGGATA CAGACGTTCT 
ATAGATGCTG ATAGAACATT ATCTCCAAGC CCTACTTCGA AAACGCCTCC ACCTATGAAA 
ATTGAAACTA CTGAATTACC GAAAATGAAC GGTGTTACAG AATCAGGAAA TATTAAAAAT 
GTTTCTTCGA TATCACCAAG TCCAACAAAT AGCCAACTAC ATCCACAGCC TGCTCCTAGG 
CACAGCATTT CGCAACCAAC AATTACACCT ACAACTACTA CGAGTTCAAC GCCCACATCT 
TCTACAGAAC CTAGGTCAAC CTCACCCCAG GAAGATATAC AGGACGTGAT TCTTGTAAAA 
GAGGGATCTC TAGGATTTAG TATTATTGGT GGTACGGATC ATTCATGCAC TCCATTTGGT 
GCAAAGGAAC CTGGAATATT TATATCTCAT GTCGTGCCTG GTGGTATAGC TGCAAAATCT 
GGTAAATTAA GAATGGGTGA TAGAATACTA AAGGTAAATG GTACTGATGT AACAAAAGCT 
ACTCATCAAG AGGCTGTGAT GGAACTTTTG CGACCAGGAG ATCAAATAGT GCTTACTGTC 
CAACACGATC CACTACCCGA AAATTATCAG CTGGTCGAAA TAGAGTACAT CCCTGTGACA 
GAATTAGTTA TTACAAAAGA ACCAGGAGAG AAACTGGGCA TGCATATTAA AGGTGGACTT 
CGAGGACAAA AAGGTAATCC ACTTGATCAT ACAGATGAAG GGGTTTTTAT ATCCAAAATC 
AACTCAGGTG GTGCAGCTAA AAGAGATGGA AGACTGAAAG TTGGAATGAG ACTATTAGAA 
GTTAATGGTA CATCATTATT AGGCGCAACT CACCAAGAAG CAGTAAATAT ATTGCGATGT 
TCAGGAAATA CGATTACTTT GGTGGTTTGT AAAGGATATG ATAAGAGTGA AATTGAACCA 
GTATTACCAA TATCCGATGG AAGAGATTCT AAGGAATCTA GAACATCCAA AGAATTAAAG 
GATGCTACAA CAGAAGGTAC TAAATCGTTA TCACAAAGTA TATCCAGTCT GGATCGCGAT 
GATGAAGAAG CAGCAACTCT TAGACAAGAA CAGGAAATGA AAGCTGAACT TGTCGCATGG 
GAACAAGAAG AAAGAGAACG TGCTTTGCTT GAGCATAGAG AAAAATCTAC TCCTGAAAAA 
GTATTAGATG TAGTAAGAGC AGCTGAATCA TTAGTAAGCA AATCAAATAG TCCGGTTGAT 
ATGGTCGTAC CACCAAAGTC ACCAGGTGGT ACGAAAGATC TCAAAACAAC CACTATTGTG 
ATGAGCAAAC ATACTCTAGC ACCTCAAAAT ACAAATTCAC TGAGGAAAGA GAGAACTGGA 
ACATCAGTGG ACAATCCATC GCCCAAGTCT CCGATCTCTA CTTTAACTCC ATCGATGAAT 
TTCGATCACA CTACTGCTGT CCAAAGCTTG CCCTCTCCAA AGAAAAAAAG TTCCATACCA 
AAACGTAGTA TTACATTTGC TGATCTTCCT CCCATTTCTA AAGAACCAAT TAATTATTCC 
ATTTCGGCGA AAGAAGATCC ATCACCTTTG CATGATAGTA AATTTACTTT CGATCCTCAA 
ACTATTTCTT ATAAGAAAGT CTATCAAAAT CCTATCCAAG CTACTCATTC TCGTTTCAAA 
CTTCCTCCTA CACCTTTAAC TGCTCCTAAA TCTATAGAAG ATTATGGTAC TTCAATTTCT 
TTTGGTAAAC GACAGAACGC ACGCTCTGTT GATGGGAATA GTCAGGTTGA GCTTTCTCCA 
ACAACATCAC CAACACCACC TCTAGTAAAG ATGTCGGTTA GTGATAAAAA AAAATTGTTT 
GAAAGTGCCA TGGAGGAACA TTTAAAACCT TCCCCTAAAC CAGAAAAAGT ATTCAGTTTT 
CTAAGTCAGG ATGAAGTTGA GAAAATGAAA CAAGAAGAAG AAAAGAAGAT TGCTACTCTA 
ACGCGGGATG AATTAAAGTC GTGGGCACAA CTTGATGAAA ATGAAGGTTT GGAAGATTTA 
GAAGAAACAT TAGAAGATCA GGATAATCGG CGACCTAATA GCCGACTGAG TTCGCGAACT 
TCGATCCCTC TTCTGCAAAA TCTTCCAAGC AGTGTAAGGA CAGCAAAAGC AGAACGCCGC 
TTGAAAGAAA GATTAATACA GGAGGGTATA ATTTCGGATG AAGATGAAGA AAGTCATTTA 
AGTCCTGCCG AACAGAGAGC GTTAAGAGCA GAAAAACGTG CGGCATGGAG ACAAGCACGT 
TTGAAATCTC TGGAACAAGA TGCTCTTCAA GCACAGATAG TAATTAAAAA AATGAGTGAA 
ATGATGGACA CGAATAAAAC AAGTTCAATG CAGGATTCTA CAGATATTCC TATTACTCCT 
GTTTCTGAAT CGGAAAAGAC AACTGATTTC ACTACGTTAC GGCCGTCTAG TGCAGATTTT 
CCCAAACTTG CTGTACGTAG CAAAGTGGGT CCCCCTAAAG AGATACGCGA ATCCGAGAAA 
GTAGTGGACG AGAAGGTCAC TCGACGTACT GAGGAATATG TGGATGAGGT GACGGGTGAA 
CGACGTGTGC GAACAGTCGA ATACGTCGAG AAACTCATTG AACGACAGGT TGAAACTCTA 
CGAGAAAAGA TAATATCATT GGAATTAAGT AATGCAGAAG ATGAAATAGA AAGTATTACC 
GGTACAGGAG CAAGTGATGC TGAAAGCGAA AGCGAAGAAA TTACTGTACA ACCTCCTACT 
ATGACTACTT TACAAGAAAG AAATGAAACA ATTATATCTA CAAATGAAGG TGCCGCACCT 
AAAATAAGTG CAAATACTGT TCTTGTTTCA AAAAAAAAGA AACGAAAACG TTCGAAGAAA 
GGTCGTCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108003564 ( XM_017065895.1 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_016921384.1 Apis cerana protein lap4 (LOC108003564), transcript variant X9, mRNA.

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CloneID	OAp29728
Clone ID Related Accession (Same CDS sequence)	XM_017065895.1 , XM_017065895.2
Accession Version	XM_017065895.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-08
Organism	Apis cerana(Asiatic honeybee)
Product	protein lap4 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016017567.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis cerana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGC AGATATCATC GAGAAAAAAC 
TCAGTGTGCT CTAGTTTAAG TACTAGTAGA GCTCCAAGTG CAACATCTCA TGTTTCATCT 
ATAGGTTTTT CACATAATTT AGAAAATGGT GATGCTTTAT CTCATGATGC TATTAAGTGT 
AAGAAAATTC CTGAACCTAT ATCTTCAACA AAAGTAGGCA ATGAACCAAT GGTATCAGTT 
CTTGTTCACA CAACATTAAT ACGGGACCAA AATGGGCTTG GATTTAGTAT TGCTGGTGGA 
GAAGGTTCTC CACCATTCAA AGACAATAGT GATGCGATAT ATATTTCAAG AATAACAGAT 
GGCGGCGTAG CACAAAAAGA TGGTAAATTA TTAGTTGGAG ACAAAGTAAT ATCTATTAAT 
GGAGTTGAGA TGAGAGGAGC TAAGCATGAA CAAGCAGTTG CTTTATTAAC AGGTTTAGAA 
AGATTTGTTC GATTAGTAGT TGAACGCGAA ATACCATTTT CGCAAGCAAA TGCAGCTACG 
GTCGTAACAC CTTCAGAAAA ATCACCACGA GTGATCGGTG CACCTAAACC ATATACAGGA 
TTATATAATG CCAATAGTTA TATAGCAAAT AAACCGGGAT ATACTGGATA CAGACGTTCT 
ATAGATGCTG ATAGAACATT ATCTCCAAGC CCTACTTCGA AAACGCCTCC ACCTATGAAA 
ATTGAAACTA CTGAATTACC GAAAATGAAC GGTGTTACAG AATCAGGAAA TATTAAAAAT 
GTTTCTTCGA TATCACCAAG TCCAACAAAT AGCCAACTAC ATCCACAGCC TGCTCCTAGG 
CACAGCATTT CGCAACCAAC AATTACACCT ACAACTACTA CGAGTTCAAC GCCCACATCT 
TCTACAGAAC CTAGGTCAAC CTCACCCCAG GAAGATATAC AGGACGTGAT TCTTGTAAAA 
GAGGGATCTC TAGGATTTAG TATTATTGGT GGTACGGATC ATTCATGCAC TCCATTTGGT 
GCAAAGGAAC CTGGAATATT TATATCTCAT GTCGTGCCTG GTGGTATAGC TGCAAAATCT 
GGTAAATTAA GAATGGGTGA TAGAATACTA AAGGTAAATG GTACTGATGT AACAAAAGCT 
ACTCATCAAG AGGCTGTGAT GGAACTTTTG CGACCAGGAG ATCAAATAGT GCTTACTGTC 
CAACACGATC CACTACCCGA AAATTATCAG GAATTAGTTA TTACAAAAGA ACCAGGAGAG 
AAACTGGGCA TGCATATTAA AGGTGGACTT CGAGGACAAA AAGGTAATCC ACTTGATCAT 
ACAGATGAAG GGGTTTTTAT ATCCAAAATC AACTCAGGTG GTGCAGCTAA AAGAGATGGA 
AGACTGAAAG TTGGAATGAG ACTATTAGAA GTTAATGGTA CATCATTATT AGGCGCAACT 
CACCAAGAAG CAGTAAATAT ATTGCGATGT TCAGGAAATA CGATTACTTT GGTGGTTTGT 
AAAGGATATG ATAAGAGTGA AATTGAACCA GTATTACCAA TATCCGATGG AAGAGATTCT 
AAGGAATCTA GAACATCCAA AGAATTAAAG GATGCTACAA CAGAAGGTAC TAAATCGTTA 
TCACAAAGTA TATCCAGTCT GGATCGCGAT GATGAAGAAG CAGCAACTCT TAGACAAGAA 
CAGGAAATGA AAGCTGAACT TGTCGCATGG GAACAAGAAG AAAGAGAACG TGCTTTGCTT 
GAGCATAGAG AAAAATCTAC TCCTGAAAAA GTATTAGATG TAGTAAGAGC AGCTGAATCA 
TTAGTAAGCA AATCAAATAG TCCGGTTGAT ATGGTCGTAC CACCAAAGTC ACCAGGTGGT 
ACGAAAGATC TCAAAACAAC CACTATTGTG ATGAGCAAAC ATACTCTAGC ACCTCAAAAT 
ACAAATTCAC TGAGGAAAGA GAGAACTGGA ACATCAGTGG ACAATCCATC GCCCAAGTCT 
CCGATCTCTA CTTTAACTCC ATCGATGAAT TTCGATCACA CTACTGCTGT CCAAAGCTTG 
CCCTCTCCAA AGAAAAAAAG TTCCATACCA AAACGTAGTA TTACATTTGC TGATCTTCCT 
CCCATTTCTA AAGAACCAAT TAATTATTCC ATTTCGGCGA AAGAAGATCC ATCACCTTTG 
CATGATAGTA AATTTACTTT CGATCCTCAA ACTATTTCTT ATAAGAAAGT CTATCAAAAT 
CCTATCCAAG CTACTCATTC TCGTTTCAAA CTTCCTCCTA CACCTTTAAC TGCTCCTAAA 
TCTATAGAAG ATTATGGTAC TTCAATTTCT TTTGGTAAAC GACAGAACGC ACGCTCTGTT 
GATGGGAATA GTCAGGTTGA GCTTTCTCCA ACAACATCAC CAACACCACC TCTAGTAAAG 
ATGTCGGTTA GTGATAAAAA AAAATTGTTT GAAAGTGCCA TGGAGGAACA TTTAAAACCT 
TCCCCTAAAC CAGAAAAAGT ATTCAGTTTT CTAAGTCAGG ATGAAGTTGA GAAAATGAAA 
CAAGAAGAAG AAAAGAAGAT TGCTACTCTA ACGCGGGATG AATTAAAGTC GTGGGCACAA 
CTTGATGAAA ATGAAGGTTT GGAAGATTTA GAAGAAACAT TAGAAGATCA GGATAATCGG 
CGACCTAATA GCCGACTGAG TTCGCGAACT TCGATCCCTC TTCTGCAAAA TCTTCCAAGC 
AGTGTAAGGA CAGCAAAAGC AGAACGCCGC TTGAAAGAAA GATTAATACA GGAGGGTATA 
ATTTCGGATG AAGATGAAGA AAGTCATTTA AGTCCTGCCG AACAGAGAGC GTTAAGAGCA 
GAAAAACGTG CGGCATGGAG ACAAGCACGT TTGAAATCTC TGGAACAAGA TGCTCTTCAA 
GCACAGATAG TAATTAAAAA AATGAGTGAA ATGATGGACA CGAATAAAAC AAGTTCAATG 
CAGGATTCTA CAGATATTCC TATTACTCCT GTTTCTGAAT CGGAAAAGAC AACTGATTTC 
ACTACGTTAC GGCCGTCTAG TGCAGATTTT CCCAAACTTG CTGTACGTAG CAAAGTGGGT 
CCCCCTAAAG AGATACGCGA ATCCGAGAAA GTAGTGGACG AGAAGGTCAC TCGACGTACT 
GAGGAATATG TGGATGAGGT GACGGGTGAA CGACGTGTGC GAACAGTCGA ATACGTCGAG 
AAACTCATTG AACGACAGGT TGAAACTCTA CGAGAAAAGA TAATATCATT GGAATTAAGT 
AATGCAGAAG ATGAAATAGA AAGTATTACC GGTACAGGAG CAAGTGATGC TGAAAGCGAA 
AGCGAAGAAA TTACTGTACA ACCTCCTACT ATGACTACTT TACAAGAAAG AAATGAAACA 
ATTATATCTA CAAATGAAGG TGCCGCACCT AAAATAAGTG CAAATACTGT TCTTGTTTCA 
AAAAAAAAGA AACGAAAACG TTCGAAGAAA GGTCGTCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016921384.1
CDS	233..6094
Translation	MFRCIPIFKGCNRQVEYIDKRHCSLPCVPDDILRYSRSLEELLLDANHIRDLPKNFFRLQKLRKLGLSDNEIHRLPPDIQNFENLVELDVSRNDIPDIPENIKNLRALQVADFSSNPIPRLPAGFVQLRNLTVLGLNDMSLTNLPPDFGSLEALQSLELRENLLKSLPESLSQLYKLERLDLGDNDIEVLPAHIGELPALQELWLDHNQLQHLPPEIGELKTLVCLDVSENRLEDLPEEIGGLEALTDLHLSQNVIEKLPDGLGELKKLTILKVDQNRLSTLNPNIGRCENLQELILTENFLLELPVSIGKLLNLNNLNVDRNSLQSLPTETGNLKQLGVLSLRDNKLQYLPIEVGQCTALHVLDVSGNRLQYLPYSLINLNLKAVWLSKNQAQPMLTFQTDVDEETGQEVLTCFLLPQLEIHPDDSGRLGMLAGMRNAVQDGEIRELGSSDDEGWQEKEASRTHSVKFTDEPPETDKETPFVRQNTPHPKELKAKAHKLFSKGKNDSRSASTDEQDVSQTFGLDKTETDVSTKPNDLTVDLIEQESLKHESVENEQKEIVPGVTDNIDVQSSNEPEIVSNQYIVESNTDVRTEGSISELKDRNDKEDDESENEETQRHVEFSIAEGTDYDGSGDSNRPNRLHRRDTPHHLKNKRIHTAVDKEKVASIIAQALMKKNDEISTSTSAPAETTENFDAQSQDAISDAEPTIEVREEQYEIHIERTTGGLGLSIAGGIGSTPFKGDDEGIFISRVTEGGPADLAGLKVEDKVLSVNGVSVVNVGHYDAVEVLKACGRVLVLVVQREVTRIVPPFEQQISSRKNSVCSSLSTSRAPSATSHVSSIGFSHNLENGDALSHDAIKCKKIPEPISSTKVGNEPMVSVLVHTTLIRDQNGLGFSIAGGEGSPPFKDNSDAIYISRITDGGVAQKDGKLLVGDKVISINGVEMRGAKHEQAVALLTGLERFVRLVVEREIPFSQANAATVVTPSEKSPRVIGAPKPYTGLYNANSYIANKPGYTGYRRSIDADRTLSPSPTSKTPPPMKIETTELPKMNGVTESGNIKNVSSISPSPTNSQLHPQPAPRHSISQPTITPTTTTSSTPTSSTEPRSTSPQEDIQDVILVKEGSLGFSIIGGTDHSCTPFGAKEPGIFISHVVPGGIAAKSGKLRMGDRILKVNGTDVTKATHQEAVMELLRPGDQIVLTVQHDPLPENYQELVITKEPGEKLGMHIKGGLRGQKGNPLDHTDEGVFISKINSGGAAKRDGRLKVGMRLLEVNGTSLLGATHQEAVNILRCSGNTITLVVCKGYDKSEIEPVLPISDGRDSKESRTSKELKDATTEGTKSLSQSISSLDRDDEEAATLRQEQEMKAELVAWEQEERERALLEHREKSTPEKVLDVVRAAESLVSKSNSPVDMVVPPKSPGGTKDLKTTTIVMSKHTLAPQNTNSLRKERTGTSVDNPSPKSPISTLTPSMNFDHTTAVQSLPSPKKKSSIPKRSITFADLPPISKEPINYSISAKEDPSPLHDSKFTFDPQTISYKKVYQNPIQATHSRFKLPPTPLTAPKSIEDYGTSISFGKRQNARSVDGNSQVELSPTTSPTPPLVKMSVSDKKKLFESAMEEHLKPSPKPEKVFSFLSQDEVEKMKQEEEKKIATLTRDELKSWAQLDENEGLEDLEETLEDQDNRRPNSRLSSRTSIPLLQNLPSSVRTAKAERRLKERLIQEGIISDEDEESHLSPAEQRALRAEKRAAWRQARLKSLEQDALQAQIVIKKMSEMMDTNKTSSMQDSTDIPITPVSESEKTTDFTTLRPSSADFPKLAVRSKVGPPKEIRESEKVVDEKVTRRTEEYVDEVTGERRVRTVEYVEKLIERQVETLREKIISLELSNAEDEIESITGTGASDAESESEEITVQPPTMTTLQERNETIISTNEGAAPKISANTVLVSKKKKRKRSKKGRH

Target ORF information:

RefSeq Version	XM_017065895.1
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana protein lap4 (LOC108003564), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017065895.1

ORF Insert Sequence:

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGC AGATATCATC GAGAAAAAAC 
TCAGTGTGCT CTAGTTTAAG TACTAGTAGA GCTCCAAGTG CAACATCTCA TGTTTCATCT 
ATAGGTTTTT CACATAATTT AGAAAATGGT GATGCTTTAT CTCATGATGC TATTAAGTGT 
AAGAAAATTC CTGAACCTAT ATCTTCAACA AAAGTAGGCA ATGAACCAAT GGTATCAGTT 
CTTGTTCACA CAACATTAAT ACGGGACCAA AATGGGCTTG GATTTAGTAT TGCTGGTGGA 
GAAGGTTCTC CACCATTCAA AGACAATAGT GATGCGATAT ATATTTCAAG AATAACAGAT 
GGCGGCGTAG CACAAAAAGA TGGTAAATTA TTAGTTGGAG ACAAAGTAAT ATCTATTAAT 
GGAGTTGAGA TGAGAGGAGC TAAGCATGAA CAAGCAGTTG CTTTATTAAC AGGTTTAGAA 
AGATTTGTTC GATTAGTAGT TGAACGCGAA ATACCATTTT CGCAAGCAAA TGCAGCTACG 
GTCGTAACAC CTTCAGAAAA ATCACCACGA GTGATCGGTG CACCTAAACC ATATACAGGA 
TTATATAATG CCAATAGTTA TATAGCAAAT AAACCGGGAT ATACTGGATA CAGACGTTCT 
ATAGATGCTG ATAGAACATT ATCTCCAAGC CCTACTTCGA AAACGCCTCC ACCTATGAAA 
ATTGAAACTA CTGAATTACC GAAAATGAAC GGTGTTACAG AATCAGGAAA TATTAAAAAT 
GTTTCTTCGA TATCACCAAG TCCAACAAAT AGCCAACTAC ATCCACAGCC TGCTCCTAGG 
CACAGCATTT CGCAACCAAC AATTACACCT ACAACTACTA CGAGTTCAAC GCCCACATCT 
TCTACAGAAC CTAGGTCAAC CTCACCCCAG GAAGATATAC AGGACGTGAT TCTTGTAAAA 
GAGGGATCTC TAGGATTTAG TATTATTGGT GGTACGGATC ATTCATGCAC TCCATTTGGT 
GCAAAGGAAC CTGGAATATT TATATCTCAT GTCGTGCCTG GTGGTATAGC TGCAAAATCT 
GGTAAATTAA GAATGGGTGA TAGAATACTA AAGGTAAATG GTACTGATGT AACAAAAGCT 
ACTCATCAAG AGGCTGTGAT GGAACTTTTG CGACCAGGAG ATCAAATAGT GCTTACTGTC 
CAACACGATC CACTACCCGA AAATTATCAG GAATTAGTTA TTACAAAAGA ACCAGGAGAG 
AAACTGGGCA TGCATATTAA AGGTGGACTT CGAGGACAAA AAGGTAATCC ACTTGATCAT 
ACAGATGAAG GGGTTTTTAT ATCCAAAATC AACTCAGGTG GTGCAGCTAA AAGAGATGGA 
AGACTGAAAG TTGGAATGAG ACTATTAGAA GTTAATGGTA CATCATTATT AGGCGCAACT 
CACCAAGAAG CAGTAAATAT ATTGCGATGT TCAGGAAATA CGATTACTTT GGTGGTTTGT 
AAAGGATATG ATAAGAGTGA AATTGAACCA GTATTACCAA TATCCGATGG AAGAGATTCT 
AAGGAATCTA GAACATCCAA AGAATTAAAG GATGCTACAA CAGAAGGTAC TAAATCGTTA 
TCACAAAGTA TATCCAGTCT GGATCGCGAT GATGAAGAAG CAGCAACTCT TAGACAAGAA 
CAGGAAATGA AAGCTGAACT TGTCGCATGG GAACAAGAAG AAAGAGAACG TGCTTTGCTT 
GAGCATAGAG AAAAATCTAC TCCTGAAAAA GTATTAGATG TAGTAAGAGC AGCTGAATCA 
TTAGTAAGCA AATCAAATAG TCCGGTTGAT ATGGTCGTAC CACCAAAGTC ACCAGGTGGT 
ACGAAAGATC TCAAAACAAC CACTATTGTG ATGAGCAAAC ATACTCTAGC ACCTCAAAAT 
ACAAATTCAC TGAGGAAAGA GAGAACTGGA ACATCAGTGG ACAATCCATC GCCCAAGTCT 
CCGATCTCTA CTTTAACTCC ATCGATGAAT TTCGATCACA CTACTGCTGT CCAAAGCTTG 
CCCTCTCCAA AGAAAAAAAG TTCCATACCA AAACGTAGTA TTACATTTGC TGATCTTCCT 
CCCATTTCTA AAGAACCAAT TAATTATTCC ATTTCGGCGA AAGAAGATCC ATCACCTTTG 
CATGATAGTA AATTTACTTT CGATCCTCAA ACTATTTCTT ATAAGAAAGT CTATCAAAAT 
CCTATCCAAG CTACTCATTC TCGTTTCAAA CTTCCTCCTA CACCTTTAAC TGCTCCTAAA 
TCTATAGAAG ATTATGGTAC TTCAATTTCT TTTGGTAAAC GACAGAACGC ACGCTCTGTT 
GATGGGAATA GTCAGGTTGA GCTTTCTCCA ACAACATCAC CAACACCACC TCTAGTAAAG 
ATGTCGGTTA GTGATAAAAA AAAATTGTTT GAAAGTGCCA TGGAGGAACA TTTAAAACCT 
TCCCCTAAAC CAGAAAAAGT ATTCAGTTTT CTAAGTCAGG ATGAAGTTGA GAAAATGAAA 
CAAGAAGAAG AAAAGAAGAT TGCTACTCTA ACGCGGGATG AATTAAAGTC GTGGGCACAA 
CTTGATGAAA ATGAAGGTTT GGAAGATTTA GAAGAAACAT TAGAAGATCA GGATAATCGG 
CGACCTAATA GCCGACTGAG TTCGCGAACT TCGATCCCTC TTCTGCAAAA TCTTCCAAGC 
AGTGTAAGGA CAGCAAAAGC AGAACGCCGC TTGAAAGAAA GATTAATACA GGAGGGTATA 
ATTTCGGATG AAGATGAAGA AAGTCATTTA AGTCCTGCCG AACAGAGAGC GTTAAGAGCA 
GAAAAACGTG CGGCATGGAG ACAAGCACGT TTGAAATCTC TGGAACAAGA TGCTCTTCAA 
GCACAGATAG TAATTAAAAA AATGAGTGAA ATGATGGACA CGAATAAAAC AAGTTCAATG 
CAGGATTCTA CAGATATTCC TATTACTCCT GTTTCTGAAT CGGAAAAGAC AACTGATTTC 
ACTACGTTAC GGCCGTCTAG TGCAGATTTT CCCAAACTTG CTGTACGTAG CAAAGTGGGT 
CCCCCTAAAG AGATACGCGA ATCCGAGAAA GTAGTGGACG AGAAGGTCAC TCGACGTACT 
GAGGAATATG TGGATGAGGT GACGGGTGAA CGACGTGTGC GAACAGTCGA ATACGTCGAG 
AAACTCATTG AACGACAGGT TGAAACTCTA CGAGAAAAGA TAATATCATT GGAATTAAGT 
AATGCAGAAG ATGAAATAGA AAGTATTACC GGTACAGGAG CAAGTGATGC TGAAAGCGAA 
AGCGAAGAAA TTACTGTACA ACCTCCTACT ATGACTACTT TACAAGAAAG AAATGAAACA 
ATTATATCTA CAAATGAAGG TGCCGCACCT AAAATAAGTG CAAATACTGT TCTTGTTTCA 
AAAAAAAAGA AACGAAAACG TTCGAAGAAA GGTCGTCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108003564 ( XM_017065895.2 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_016921384.1 Apis cerana protein lap4 (LOC108003564), transcript variant X9, mRNA.

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CloneID	OAp29728
Clone ID Related Accession (Same CDS sequence)	XM_017065895.1 , XM_017065895.2
Accession Version	XM_017065895.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-16
Organism	Apis cerana(Asiatic honeybee)
Product	protein lap4 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016017567.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 17, 2019 this sequence version replaced XM_017065895.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis cerana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGC AGATATCATC GAGAAAAAAC 
TCAGTGTGCT CTAGTTTAAG TACTAGTAGA GCTCCAAGTG CAACATCTCA TGTTTCATCT 
ATAGGTTTTT CACATAATTT AGAAAATGGT GATGCTTTAT CTCATGATGC TATTAAGTGT 
AAGAAAATTC CTGAACCTAT ATCTTCAACA AAAGTAGGCA ATGAACCAAT GGTATCAGTT 
CTTGTTCACA CAACATTAAT ACGGGACCAA AATGGGCTTG GATTTAGTAT TGCTGGTGGA 
GAAGGTTCTC CACCATTCAA AGACAATAGT GATGCGATAT ATATTTCAAG AATAACAGAT 
GGCGGCGTAG CACAAAAAGA TGGTAAATTA TTAGTTGGAG ACAAAGTAAT ATCTATTAAT 
GGAGTTGAGA TGAGAGGAGC TAAGCATGAA CAAGCAGTTG CTTTATTAAC AGGTTTAGAA 
AGATTTGTTC GATTAGTAGT TGAACGCGAA ATACCATTTT CGCAAGCAAA TGCAGCTACG 
GTCGTAACAC CTTCAGAAAA ATCACCACGA GTGATCGGTG CACCTAAACC ATATACAGGA 
TTATATAATG CCAATAGTTA TATAGCAAAT AAACCGGGAT ATACTGGATA CAGACGTTCT 
ATAGATGCTG ATAGAACATT ATCTCCAAGC CCTACTTCGA AAACGCCTCC ACCTATGAAA 
ATTGAAACTA CTGAATTACC GAAAATGAAC GGTGTTACAG AATCAGGAAA TATTAAAAAT 
GTTTCTTCGA TATCACCAAG TCCAACAAAT AGCCAACTAC ATCCACAGCC TGCTCCTAGG 
CACAGCATTT CGCAACCAAC AATTACACCT ACAACTACTA CGAGTTCAAC GCCCACATCT 
TCTACAGAAC CTAGGTCAAC CTCACCCCAG GAAGATATAC AGGACGTGAT TCTTGTAAAA 
GAGGGATCTC TAGGATTTAG TATTATTGGT GGTACGGATC ATTCATGCAC TCCATTTGGT 
GCAAAGGAAC CTGGAATATT TATATCTCAT GTCGTGCCTG GTGGTATAGC TGCAAAATCT 
GGTAAATTAA GAATGGGTGA TAGAATACTA AAGGTAAATG GTACTGATGT AACAAAAGCT 
ACTCATCAAG AGGCTGTGAT GGAACTTTTG CGACCAGGAG ATCAAATAGT GCTTACTGTC 
CAACACGATC CACTACCCGA AAATTATCAG GAATTAGTTA TTACAAAAGA ACCAGGAGAG 
AAACTGGGCA TGCATATTAA AGGTGGACTT CGAGGACAAA AAGGTAATCC ACTTGATCAT 
ACAGATGAAG GGGTTTTTAT ATCCAAAATC AACTCAGGTG GTGCAGCTAA AAGAGATGGA 
AGACTGAAAG TTGGAATGAG ACTATTAGAA GTTAATGGTA CATCATTATT AGGCGCAACT 
CACCAAGAAG CAGTAAATAT ATTGCGATGT TCAGGAAATA CGATTACTTT GGTGGTTTGT 
AAAGGATATG ATAAGAGTGA AATTGAACCA GTATTACCAA TATCCGATGG AAGAGATTCT 
AAGGAATCTA GAACATCCAA AGAATTAAAG GATGCTACAA CAGAAGGTAC TAAATCGTTA 
TCACAAAGTA TATCCAGTCT GGATCGCGAT GATGAAGAAG CAGCAACTCT TAGACAAGAA 
CAGGAAATGA AAGCTGAACT TGTCGCATGG GAACAAGAAG AAAGAGAACG TGCTTTGCTT 
GAGCATAGAG AAAAATCTAC TCCTGAAAAA GTATTAGATG TAGTAAGAGC AGCTGAATCA 
TTAGTAAGCA AATCAAATAG TCCGGTTGAT ATGGTCGTAC CACCAAAGTC ACCAGGTGGT 
ACGAAAGATC TCAAAACAAC CACTATTGTG ATGAGCAAAC ATACTCTAGC ACCTCAAAAT 
ACAAATTCAC TGAGGAAAGA GAGAACTGGA ACATCAGTGG ACAATCCATC GCCCAAGTCT 
CCGATCTCTA CTTTAACTCC ATCGATGAAT TTCGATCACA CTACTGCTGT CCAAAGCTTG 
CCCTCTCCAA AGAAAAAAAG TTCCATACCA AAACGTAGTA TTACATTTGC TGATCTTCCT 
CCCATTTCTA AAGAACCAAT TAATTATTCC ATTTCGGCGA AAGAAGATCC ATCACCTTTG 
CATGATAGTA AATTTACTTT CGATCCTCAA ACTATTTCTT ATAAGAAAGT CTATCAAAAT 
CCTATCCAAG CTACTCATTC TCGTTTCAAA CTTCCTCCTA CACCTTTAAC TGCTCCTAAA 
TCTATAGAAG ATTATGGTAC TTCAATTTCT TTTGGTAAAC GACAGAACGC ACGCTCTGTT 
GATGGGAATA GTCAGGTTGA GCTTTCTCCA ACAACATCAC CAACACCACC TCTAGTAAAG 
ATGTCGGTTA GTGATAAAAA AAAATTGTTT GAAAGTGCCA TGGAGGAACA TTTAAAACCT 
TCCCCTAAAC CAGAAAAAGT ATTCAGTTTT CTAAGTCAGG ATGAAGTTGA GAAAATGAAA 
CAAGAAGAAG AAAAGAAGAT TGCTACTCTA ACGCGGGATG AATTAAAGTC GTGGGCACAA 
CTTGATGAAA ATGAAGGTTT GGAAGATTTA GAAGAAACAT TAGAAGATCA GGATAATCGG 
CGACCTAATA GCCGACTGAG TTCGCGAACT TCGATCCCTC TTCTGCAAAA TCTTCCAAGC 
AGTGTAAGGA CAGCAAAAGC AGAACGCCGC TTGAAAGAAA GATTAATACA GGAGGGTATA 
ATTTCGGATG AAGATGAAGA AAGTCATTTA AGTCCTGCCG AACAGAGAGC GTTAAGAGCA 
GAAAAACGTG CGGCATGGAG ACAAGCACGT TTGAAATCTC TGGAACAAGA TGCTCTTCAA 
GCACAGATAG TAATTAAAAA AATGAGTGAA ATGATGGACA CGAATAAAAC AAGTTCAATG 
CAGGATTCTA CAGATATTCC TATTACTCCT GTTTCTGAAT CGGAAAAGAC AACTGATTTC 
ACTACGTTAC GGCCGTCTAG TGCAGATTTT CCCAAACTTG CTGTACGTAG CAAAGTGGGT 
CCCCCTAAAG AGATACGCGA ATCCGAGAAA GTAGTGGACG AGAAGGTCAC TCGACGTACT 
GAGGAATATG TGGATGAGGT GACGGGTGAA CGACGTGTGC GAACAGTCGA ATACGTCGAG 
AAACTCATTG AACGACAGGT TGAAACTCTA CGAGAAAAGA TAATATCATT GGAATTAAGT 
AATGCAGAAG ATGAAATAGA AAGTATTACC GGTACAGGAG CAAGTGATGC TGAAAGCGAA 
AGCGAAGAAA TTACTGTACA ACCTCCTACT ATGACTACTT TACAAGAAAG AAATGAAACA 
ATTATATCTA CAAATGAAGG TGCCGCACCT AAAATAAGTG CAAATACTGT TCTTGTTTCA 
AAAAAAAAGA AACGAAAACG TTCGAAGAAA GGTCGTCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016921384.1
CDS	1475..7336
Translation	MFRCIPIFKGCNRQVEYIDKRHCSLPCVPDDILRYSRSLEELLLDANHIRDLPKNFFRLQKLRKLGLSDNEIHRLPPDIQNFENLVELDVSRNDIPDIPENIKNLRALQVADFSSNPIPRLPAGFVQLRNLTVLGLNDMSLTNLPPDFGSLEALQSLELRENLLKSLPESLSQLYKLERLDLGDNDIEVLPAHIGELPALQELWLDHNQLQHLPPEIGELKTLVCLDVSENRLEDLPEEIGGLEALTDLHLSQNVIEKLPDGLGELKKLTILKVDQNRLSTLNPNIGRCENLQELILTENFLLELPVSIGKLLNLNNLNVDRNSLQSLPTETGNLKQLGVLSLRDNKLQYLPIEVGQCTALHVLDVSGNRLQYLPYSLINLNLKAVWLSKNQAQPMLTFQTDVDEETGQEVLTCFLLPQLEIHPDDSGRLGMLAGMRNAVQDGEIRELGSSDDEGWQEKEASRTHSVKFTDEPPETDKETPFVRQNTPHPKELKAKAHKLFSKGKNDSRSASTDEQDVSQTFGLDKTETDVSTKPNDLTVDLIEQESLKHESVENEQKEIVPGVTDNIDVQSSNEPEIVSNQYIVESNTDVRTEGSISELKDRNDKEDDESENEETQRHVEFSIAEGTDYDGSGDSNRPNRLHRRDTPHHLKNKRIHTAVDKEKVASIIAQALMKKNDEISTSTSAPAETTENFDAQSQDAISDAEPTIEVREEQYEIHIERTTGGLGLSIAGGIGSTPFKGDDEGIFISRVTEGGPADLAGLKVEDKVLSVNGVSVVNVGHYDAVEVLKACGRVLVLVVQREVTRIVPPFEQQISSRKNSVCSSLSTSRAPSATSHVSSIGFSHNLENGDALSHDAIKCKKIPEPISSTKVGNEPMVSVLVHTTLIRDQNGLGFSIAGGEGSPPFKDNSDAIYISRITDGGVAQKDGKLLVGDKVISINGVEMRGAKHEQAVALLTGLERFVRLVVEREIPFSQANAATVVTPSEKSPRVIGAPKPYTGLYNANSYIANKPGYTGYRRSIDADRTLSPSPTSKTPPPMKIETTELPKMNGVTESGNIKNVSSISPSPTNSQLHPQPAPRHSISQPTITPTTTTSSTPTSSTEPRSTSPQEDIQDVILVKEGSLGFSIIGGTDHSCTPFGAKEPGIFISHVVPGGIAAKSGKLRMGDRILKVNGTDVTKATHQEAVMELLRPGDQIVLTVQHDPLPENYQELVITKEPGEKLGMHIKGGLRGQKGNPLDHTDEGVFISKINSGGAAKRDGRLKVGMRLLEVNGTSLLGATHQEAVNILRCSGNTITLVVCKGYDKSEIEPVLPISDGRDSKESRTSKELKDATTEGTKSLSQSISSLDRDDEEAATLRQEQEMKAELVAWEQEERERALLEHREKSTPEKVLDVVRAAESLVSKSNSPVDMVVPPKSPGGTKDLKTTTIVMSKHTLAPQNTNSLRKERTGTSVDNPSPKSPISTLTPSMNFDHTTAVQSLPSPKKKSSIPKRSITFADLPPISKEPINYSISAKEDPSPLHDSKFTFDPQTISYKKVYQNPIQATHSRFKLPPTPLTAPKSIEDYGTSISFGKRQNARSVDGNSQVELSPTTSPTPPLVKMSVSDKKKLFESAMEEHLKPSPKPEKVFSFLSQDEVEKMKQEEEKKIATLTRDELKSWAQLDENEGLEDLEETLEDQDNRRPNSRLSSRTSIPLLQNLPSSVRTAKAERRLKERLIQEGIISDEDEESHLSPAEQRALRAEKRAAWRQARLKSLEQDALQAQIVIKKMSEMMDTNKTSSMQDSTDIPITPVSESEKTTDFTTLRPSSADFPKLAVRSKVGPPKEIRESEKVVDEKVTRRTEEYVDEVTGERRVRTVEYVEKLIERQVETLREKIISLELSNAEDEIESITGTGASDAESESEEITVQPPTMTTLQERNETIISTNEGAAPKISANTVLVSKKKKRKRSKKGRH

Target ORF information:

RefSeq Version	XM_017065895.2
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana protein lap4 (LOC108003564), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017065895.2

ORF Insert Sequence:

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGC AGATATCATC GAGAAAAAAC 
TCAGTGTGCT CTAGTTTAAG TACTAGTAGA GCTCCAAGTG CAACATCTCA TGTTTCATCT 
ATAGGTTTTT CACATAATTT AGAAAATGGT GATGCTTTAT CTCATGATGC TATTAAGTGT 
AAGAAAATTC CTGAACCTAT ATCTTCAACA AAAGTAGGCA ATGAACCAAT GGTATCAGTT 
CTTGTTCACA CAACATTAAT ACGGGACCAA AATGGGCTTG GATTTAGTAT TGCTGGTGGA 
GAAGGTTCTC CACCATTCAA AGACAATAGT GATGCGATAT ATATTTCAAG AATAACAGAT 
GGCGGCGTAG CACAAAAAGA TGGTAAATTA TTAGTTGGAG ACAAAGTAAT ATCTATTAAT 
GGAGTTGAGA TGAGAGGAGC TAAGCATGAA CAAGCAGTTG CTTTATTAAC AGGTTTAGAA 
AGATTTGTTC GATTAGTAGT TGAACGCGAA ATACCATTTT CGCAAGCAAA TGCAGCTACG 
GTCGTAACAC CTTCAGAAAA ATCACCACGA GTGATCGGTG CACCTAAACC ATATACAGGA 
TTATATAATG CCAATAGTTA TATAGCAAAT AAACCGGGAT ATACTGGATA CAGACGTTCT 
ATAGATGCTG ATAGAACATT ATCTCCAAGC CCTACTTCGA AAACGCCTCC ACCTATGAAA 
ATTGAAACTA CTGAATTACC GAAAATGAAC GGTGTTACAG AATCAGGAAA TATTAAAAAT 
GTTTCTTCGA TATCACCAAG TCCAACAAAT AGCCAACTAC ATCCACAGCC TGCTCCTAGG 
CACAGCATTT CGCAACCAAC AATTACACCT ACAACTACTA CGAGTTCAAC GCCCACATCT 
TCTACAGAAC CTAGGTCAAC CTCACCCCAG GAAGATATAC AGGACGTGAT TCTTGTAAAA 
GAGGGATCTC TAGGATTTAG TATTATTGGT GGTACGGATC ATTCATGCAC TCCATTTGGT 
GCAAAGGAAC CTGGAATATT TATATCTCAT GTCGTGCCTG GTGGTATAGC TGCAAAATCT 
GGTAAATTAA GAATGGGTGA TAGAATACTA AAGGTAAATG GTACTGATGT AACAAAAGCT 
ACTCATCAAG AGGCTGTGAT GGAACTTTTG CGACCAGGAG ATCAAATAGT GCTTACTGTC 
CAACACGATC CACTACCCGA AAATTATCAG GAATTAGTTA TTACAAAAGA ACCAGGAGAG 
AAACTGGGCA TGCATATTAA AGGTGGACTT CGAGGACAAA AAGGTAATCC ACTTGATCAT 
ACAGATGAAG GGGTTTTTAT ATCCAAAATC AACTCAGGTG GTGCAGCTAA AAGAGATGGA 
AGACTGAAAG TTGGAATGAG ACTATTAGAA GTTAATGGTA CATCATTATT AGGCGCAACT 
CACCAAGAAG CAGTAAATAT ATTGCGATGT TCAGGAAATA CGATTACTTT GGTGGTTTGT 
AAAGGATATG ATAAGAGTGA AATTGAACCA GTATTACCAA TATCCGATGG AAGAGATTCT 
AAGGAATCTA GAACATCCAA AGAATTAAAG GATGCTACAA CAGAAGGTAC TAAATCGTTA 
TCACAAAGTA TATCCAGTCT GGATCGCGAT GATGAAGAAG CAGCAACTCT TAGACAAGAA 
CAGGAAATGA AAGCTGAACT TGTCGCATGG GAACAAGAAG AAAGAGAACG TGCTTTGCTT 
GAGCATAGAG AAAAATCTAC TCCTGAAAAA GTATTAGATG TAGTAAGAGC AGCTGAATCA 
TTAGTAAGCA AATCAAATAG TCCGGTTGAT ATGGTCGTAC CACCAAAGTC ACCAGGTGGT 
ACGAAAGATC TCAAAACAAC CACTATTGTG ATGAGCAAAC ATACTCTAGC ACCTCAAAAT 
ACAAATTCAC TGAGGAAAGA GAGAACTGGA ACATCAGTGG ACAATCCATC GCCCAAGTCT 
CCGATCTCTA CTTTAACTCC ATCGATGAAT TTCGATCACA CTACTGCTGT CCAAAGCTTG 
CCCTCTCCAA AGAAAAAAAG TTCCATACCA AAACGTAGTA TTACATTTGC TGATCTTCCT 
CCCATTTCTA AAGAACCAAT TAATTATTCC ATTTCGGCGA AAGAAGATCC ATCACCTTTG 
CATGATAGTA AATTTACTTT CGATCCTCAA ACTATTTCTT ATAAGAAAGT CTATCAAAAT 
CCTATCCAAG CTACTCATTC TCGTTTCAAA CTTCCTCCTA CACCTTTAAC TGCTCCTAAA 
TCTATAGAAG ATTATGGTAC TTCAATTTCT TTTGGTAAAC GACAGAACGC ACGCTCTGTT 
GATGGGAATA GTCAGGTTGA GCTTTCTCCA ACAACATCAC CAACACCACC TCTAGTAAAG 
ATGTCGGTTA GTGATAAAAA AAAATTGTTT GAAAGTGCCA TGGAGGAACA TTTAAAACCT 
TCCCCTAAAC CAGAAAAAGT ATTCAGTTTT CTAAGTCAGG ATGAAGTTGA GAAAATGAAA 
CAAGAAGAAG AAAAGAAGAT TGCTACTCTA ACGCGGGATG AATTAAAGTC GTGGGCACAA 
CTTGATGAAA ATGAAGGTTT GGAAGATTTA GAAGAAACAT TAGAAGATCA GGATAATCGG 
CGACCTAATA GCCGACTGAG TTCGCGAACT TCGATCCCTC TTCTGCAAAA TCTTCCAAGC 
AGTGTAAGGA CAGCAAAAGC AGAACGCCGC TTGAAAGAAA GATTAATACA GGAGGGTATA 
ATTTCGGATG AAGATGAAGA AAGTCATTTA AGTCCTGCCG AACAGAGAGC GTTAAGAGCA 
GAAAAACGTG CGGCATGGAG ACAAGCACGT TTGAAATCTC TGGAACAAGA TGCTCTTCAA 
GCACAGATAG TAATTAAAAA AATGAGTGAA ATGATGGACA CGAATAAAAC AAGTTCAATG 
CAGGATTCTA CAGATATTCC TATTACTCCT GTTTCTGAAT CGGAAAAGAC AACTGATTTC 
ACTACGTTAC GGCCGTCTAG TGCAGATTTT CCCAAACTTG CTGTACGTAG CAAAGTGGGT 
CCCCCTAAAG AGATACGCGA ATCCGAGAAA GTAGTGGACG AGAAGGTCAC TCGACGTACT 
GAGGAATATG TGGATGAGGT GACGGGTGAA CGACGTGTGC GAACAGTCGA ATACGTCGAG 
AAACTCATTG AACGACAGGT TGAAACTCTA CGAGAAAAGA TAATATCATT GGAATTAAGT 
AATGCAGAAG ATGAAATAGA AAGTATTACC GGTACAGGAG CAAGTGATGC TGAAAGCGAA 
AGCGAAGAAA TTACTGTACA ACCTCCTACT ATGACTACTT TACAAGAAAG AAATGAAACA 
ATTATATCTA CAAATGAAGG TGCCGCACCT AAAATAAGTG CAAATACTGT TCTTGTTTCA 
AAAAAAAAGA AACGAAAACG TTCGAAGAAA GGTCGTCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108003564 ( XM_017065896.1 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_016921385.1 Apis cerana protein lap4 (LOC108003564), transcript variant X10, mRNA.

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CloneID	OAp29729
Clone ID Related Accession (Same CDS sequence)	XM_017065896.1
Accession Version	XM_017065896.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-08
Organism	Apis cerana(Asiatic honeybee)
Product	protein lap4 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016017567.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis cerana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTTA CGAATCTACC GCCTGATTTC GGAAGCTTAG AGGCGTTACA ATCATTGGAG 
TTAAGGGAAA ATTTGCTCAA GTCCTTGCCA GAATCGCTAT CCCAACTTTA TAAATTGGAG 
CGGCTAGATC TTGGTGACAA TGACATTGAA GTTTTACCAG CACATATAGG AGAATTACCA 
GCATTACAAG AATTATGGCT GGATCATAAT CAGTTACAAC ATTTACCACC AGAAATAGGA 
GAATTGAAAA CGTTAGTGTG TCTGGATGTA TCTGAAAATC GTTTGGAAGA TCTCCCAGAA 
GAAATAGGAG GTCTAGAGGC TTTGACGGAT CTTCATTTAT CGCAAAATGT TATTGAAAAA 
TTACCTGATG GGCTTGGAGA ATTAAAGAAA CTTACTATTC TTAAAGTCGA TCAAAATAGG 
CTTTCTACTC TAAATCCAAA TATAGGCAGG TGTGAAAATT TACAAGAATT AATTCTTACG 
GAAAATTTTC TCTTAGAACT GCCTGTATCT ATAGGAAAAC TTCTTAATCT AAATAATTTG 
AATGTAGATA GGAATAGTTT GCAATCACTT CCTACGGAAA CGGGAAATTT AAAACAATTG 
GGTGTACTGT CCTTAAGAGA TAATAAATTA CAATATCTTC CAATCGAAGT TGGACAATGT 
ACTGCTCTCC ATGTATTGGA TGTGTCTGGC AATAGATTAC AATACTTGCC ATATTCCTTA 
ATCAATTTGA ACTTGAAGGC AGTATGGTTA AGTAAAAATC AAGCACAACC AATGCTTACT 
TTTCAGACAG ATGTTGATGA AGAAACTGGA CAAGAAGTAT TAACCTGTTT TCTTTTACCA 
CAATTAGAAA TTCATCCAGA TGATTCAGGA AGACTTGGTA TGCTTGCTGG AATGAGAAAT 
GCTGTTCAAG ATGGTGAAAT ACGTGAACTT GGTAGTAGTG ATGATGAAGG ATGGCAAGAA 
AAAGAAGCAT CAAGAACGCA TTCTGTCAAG TTTACAGATG AACCTCCAGA AACTGATAAG 
GAGACACCTT TTGTACGACA AAATACACCT CATCCCAAAG AATTAAAAGC AAAAGCTCAT 
AAATTATTTA GTAAAGGAAA AAATGACAGT CGTTCAGCAT CTACAGATGA GCAAGATGTT 
TCTCAAACAT TTGGTTTGGA CAAAACTGAA ACTGATGTTT CAACAAAACC TAACGATTTG 
ACAGTTGATT TGATAGAACA AGAATCATTA AAACATGAAT CTGTAGAAAA TGAACAAAAA 
GAAATTGTAC CTGGAGTAAC TGATAATATA GATGTTCAAT CTAGTAATGA ACCAGAAATA 
GTTTCTAATC AATATATAGT AGAATCTAAT ACTGACGTAA GAACTGAAGG TTCAATATCT 
GAGTTAAAGG ATAGAAATGA TAAGGAGGAT GATGAATCTG AAAATGAAGA AACACAAAGA 
CATGTAGAAT TTTCTATAGC TGAAGGTACA GATTATGATG GTAGTGGTGA TTCAAATAGA 
CCAAATAGAC TTCATCGTAG AGATACTCCA CATCATTTGA AAAACAAGCG AATTCATACA 
GCAGTGGATA AAGAAAAAGT AGCATCCATT ATTGCACAAG CACTCATGAA AAAAAATGAT 
GAAATTTCCA CATCCACATC AGCACCTGCA GAAACTACAG AAAATTTTGA TGCTCAAAGT 
CAAGATGCGA TTTCAGATGC TGAGCCGACA ATAGAAGTAC GAGAAGAACA ATACGAAATT 
CACATCGAAA GAACAACAGG TGGTCTAGGC TTATCAATAG CTGGTGGTAT TGGTTCAACC 
CCTTTTAAAG GCGATGATGA AGGCATTTTT ATTTCCAGAG TTACTGAAGG TGGACCTGCA 
GATTTAGCAG GTTTAAAAGT GGAAGATAAA GTATTATCTG TTAATGGTGT ATCAGTAGTA 
AATGTAGGTC ATTATGATGC TGTAGAAGTT TTGAAAGCTT GTGGACGAGT TCTTGTATTA 
GTAGTTCAAA GAGAAGTTAC AAGGATAGTG CCTCCATTTG AACAGCAGAT ATCATCGAGA 
AAAAACTCAG TGTGCTCTAG TTTAAGTACT AGTAGAGCTC CAAGTGCAAC ATCTCATGTT 
TCATCTATAG GTTTTTCACA TAATTTAGAA AATGGTGATG CTTTATCTCA TGATGCTATT 
AAGTGTAAGA AAATTCCTGA ACCTATATCT TCAACAAAAG TAGGCAATGA ACCAATGGTA 
TCAGTTCTTG TTCACACAAC ATTAATACGG GACCAAAATG GGCTTGGATT TAGTATTGCT 
GGTGGAGAAG GTTCTCCACC ATTCAAAGAC AATAGTGATG CGATATATAT TTCAAGAATA 
ACAGATGGCG GCGTAGCACA AAAAGATGGT AAATTATTAG TTGGAGACAA AGTAATATCT 
ATTAATGGAG TTGAGATGAG AGGAGCTAAG CATGAACAAG CAGTTGCTTT ATTAACAGGT 
TTAGAAAGAT TTGTTCGATT AGTAGTTGAA CGCGAAATAC CATTTTCGCA AGCAAATGCA 
GCTACGGTCG TAACACCTTC AGAAAAATCA CCACGAGTGA TCGGTGCACC TAAACCATAT 
ACAGGATTAT ATAATGCCAA TAGTTATATA GCAAATAAAC CGGGATATAC TGGATACAGA 
CGTTCTATAG ATGCTGATAG AACATTATCT CCAAGCCCTA CTTCGAAAAC GCCTCCACCT 
ATGAAAATTG AAACTACTGA ATTACCGAAA ATGAACGGTG TTACAGAATC AGGAAATATT 
AAAAATGTTT CTTCGATATC ACCAAGTCCA ACAAATAGCC AACTACATCC ACAGCCTGCT 
CCTAGGCACA GCATTTCGCA ACCAACAATT ACACCTACAA CTACTACGAG TTCAACGCCC 
ACATCTTCTA CAGAACCTAG GTCAACCTCA CCCCAGGAAG ATATACAGGT ACCCAAGCCT 
ATCACCAATG AAGAATTTCA GGCTATGATT CCTGCTCATT TCCTTCGTCC TCCTACTTCC 
TCACCATCGC CAGATTCCCA TCAAGGCCCT ATCGTGACAG TGACAATAAA ACAGCCTGAT 
AATCTACCTG GAGATGTTAA TTTTCCTCCG GCTCCTACAA CACTTGGTAA AGTTACTGAG 
ACCATCACAA AGAGCACTCT CACCGAGACC GTCGTAACTA GGGTGACTGA AAATCAATTG 
GTACCACCAG TAATTATTGA GGACGTGATT CTTGTAAAAG AGGGATCTCT AGGATTTAGT 
ATTATTGGTG GTACGGATCA TTCATGCACT CCATTTGGTG CAAAGGAACC TGGAATATTT 
ATATCTCATG TCGTGCCTGG TGGTATAGCT GCAAAATCTG GTAAATTAAG AATGGGTGAT 
AGAATACTAA AGGTAAATGG TACTGATGTA ACAAAAGCTA CTCATCAAGA GGCTGTGATG 
GAACTTTTGC GACCAGGAGA TCAAATAGTG CTTACTGTCC AACACGATCC ACTACCCGAA 
AATTATCAGC TGGTCGAAAT AGAGTACATC CCTGTGACAG AATTAGTTAT TACAAAAGAA 
CCAGGAGAGA AACTGGGCAT GCATATTAAA GGTGGACTTC GAGGACAAAA AGGTAATCCA 
CTTGATCATA CAGATGAAGG GGTTTTTATA TCCAAAATCA ACTCAGGTGG TGCAGCTAAA 
AGAGATGGAA GACTGAAAGT TGGAATGAGA CTATTAGAAG TTAATGGTAC ATCATTATTA 
GGCGCAACTC ACCAAGAAGC AGTAAATATA TTGCGATGTT CAGGAAATAC GATTACTTTG 
GTGGTTTGTA AAGGATATGA TAAGAGTGAA ATTGAACCAG TATTACCAAT ATCCGATGGA 
AGAGATTCTA AGGAATCTAG AACATCCAAA GAATTAAAGG ATGCTACAAC AGAAGGTACT 
AAATCGTTAT CACAAAGTAT ATCCAGTCTG GATCGCGATG ATGAAGAAGC AGCAACTCTT 
AGACAAGAAC AGGAAATGAA AGCTGAACTT GTCGCATGGG AACAAGAAGA AAGAGAACGT 
GCTTTGCTTG AGCATAGAGA AAAATCTACT CCTGAAAAAG TATTAGATGT AGTAAGAGCA 
GCTGAATCAT TAGTAAGCAA ATCAAATAGT CCGGTTGATA TGGTCGTACC ACCAAAGTCA 
CCAGGTGGTA CGAAAGATCT CAAAACAACC ACTATTGTGA TGAGCAAACA TACTCTAGCA 
CCTCAAAATA CAAATTCACT GAGGAAAGAG AGAACTGGAA CATCAGTGGA CAATCCATCG 
CCCAAGTCTC CGATCTCTAC TTTAACTCCA TCGATGAATT TCGATCACAC TACTGCTGTC 
CAAAGCTTGC CCTCTCCAAA GAAAAAAAGT TCCATACCAA AACGTAGTAT TACATTTGCT 
GATCTTCCTC CCATTTCTAA AGAACCAATT AATTATTCCA TTTCGGCGAA AGAAGATCCA 
TCACCTTTGC ATGATAGTAA ATTTACTTTC GATCCTCAAA CTATTTCTTA TAAGAAAGTC 
TATCAAAATC CTATCCAAGC TACTCATTCT CGTTTCAAAC TTCCTCCTAC ACCTTTAACT 
GCTCCTAAAT CTATAGAAGA TTATGGTACT TCAATTTCTT TTGGTAAACG ACAGAACGCA 
CGCTCTGTTG ATGGGAATAG TCAGGTTGAG CTTTCTCCAA CAACATCACC AACACCACCT 
CTAGTAAAGA TGTCGGTTAG TGATAAAAAA AAATTGTTTG AAAGTGCCAT GGAGGAACAT 
TTAAAACCTT CCCCTAAACC AGAAAAAGTA TTCAGTTTTC TAAGTCAGGA TGAAGTTGAG 
AAAATGAAAC AAGAAGAAGA AAAGAAGATT GCTACTCTAA CGCGGGATGA ATTAAAGTCG 
TGGGCACAAC TTGATGAAAA TGAAGGTTTG GAAGATTTAG AAGAAACATT AGAAGATCAG 
GATAATCGGC GACCTAATAG CCGACTGAGT TCGCGAACTT CGATCCCTCT TCTGCAAAAT 
CTTCCAAGCA GTGTAAGGAC AGCAAAAGCA GAACGCCGCT TGAAAGAAAG ATTAATACAG 
GAGGGTATAA TTTCGGATGA AGATGAAGAA AGTCATTTAA GTCCTGCCGA ACAGAGAGCG 
TTAAGAGCAG AAAAACGTGC GGCATGGAGA CAAGCACGTT TGAAATCTCT GGAACAAGAT 
GCTCTTCAAG CACAGATAGT AATTAAAAAA ATGAGTGAAA TGATGGACAC GAATAAAACA 
AGTTCAATGC AGGATTCTAC AGATATTCCT ATTACTCCTG TTTCTGAATC GGAAAAGACA 
ACTGATTTCA CTACGTTACG GCCGTCTAGT GCAGATTTTC CCAAACTTGC TGTACGTAGC 
AAAGTGGGTC CCCCTAAAGA GATACGCGAA TCCGAGAAAG TAGTGGACGA GAAGGTCACT 
CGACGTACTG AGGAATATGT GGATGAGGTG ACGGGTGAAC GACGTGTGCG AACAGTCGAA 
TACGTCGAGA AACTCATTGA ACGACAGGTT GAAACTCTAC GAGAAAAGAT AATATCATTG 
GAATTAAGTA ATGCAGAAGA TGAAATAGAA AGTATTACCG GTACAGGAGC AAGTGATGCT 
GAAAGCGAAA GCGAAGAAAT TACTGTACAA CCTCCTACTA TGACTACTTT ACAAGAAAGA 
AATGAAACAA TTATATCTAC AAATGAAGGT GCCGCACCTA AAATAAGTGC AAATACTGTT 
CTTGTTTCAA AAAAAAAGAA ACGAAAACGT TCGAAGAAAG GTCGTCATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016921385.1
CDS	247..5997
Translation	MSLTNLPPDFGSLEALQSLELRENLLKSLPESLSQLYKLERLDLGDNDIEVLPAHIGELPALQELWLDHNQLQHLPPEIGELKTLVCLDVSENRLEDLPEEIGGLEALTDLHLSQNVIEKLPDGLGELKKLTILKVDQNRLSTLNPNIGRCENLQELILTENFLLELPVSIGKLLNLNNLNVDRNSLQSLPTETGNLKQLGVLSLRDNKLQYLPIEVGQCTALHVLDVSGNRLQYLPYSLINLNLKAVWLSKNQAQPMLTFQTDVDEETGQEVLTCFLLPQLEIHPDDSGRLGMLAGMRNAVQDGEIRELGSSDDEGWQEKEASRTHSVKFTDEPPETDKETPFVRQNTPHPKELKAKAHKLFSKGKNDSRSASTDEQDVSQTFGLDKTETDVSTKPNDLTVDLIEQESLKHESVENEQKEIVPGVTDNIDVQSSNEPEIVSNQYIVESNTDVRTEGSISELKDRNDKEDDESENEETQRHVEFSIAEGTDYDGSGDSNRPNRLHRRDTPHHLKNKRIHTAVDKEKVASIIAQALMKKNDEISTSTSAPAETTENFDAQSQDAISDAEPTIEVREEQYEIHIERTTGGLGLSIAGGIGSTPFKGDDEGIFISRVTEGGPADLAGLKVEDKVLSVNGVSVVNVGHYDAVEVLKACGRVLVLVVQREVTRIVPPFEQQISSRKNSVCSSLSTSRAPSATSHVSSIGFSHNLENGDALSHDAIKCKKIPEPISSTKVGNEPMVSVLVHTTLIRDQNGLGFSIAGGEGSPPFKDNSDAIYISRITDGGVAQKDGKLLVGDKVISINGVEMRGAKHEQAVALLTGLERFVRLVVEREIPFSQANAATVVTPSEKSPRVIGAPKPYTGLYNANSYIANKPGYTGYRRSIDADRTLSPSPTSKTPPPMKIETTELPKMNGVTESGNIKNVSSISPSPTNSQLHPQPAPRHSISQPTITPTTTTSSTPTSSTEPRSTSPQEDIQVPKPITNEEFQAMIPAHFLRPPTSSPSPDSHQGPIVTVTIKQPDNLPGDVNFPPAPTTLGKVTETITKSTLTETVVTRVTENQLVPPVIIEDVILVKEGSLGFSIIGGTDHSCTPFGAKEPGIFISHVVPGGIAAKSGKLRMGDRILKVNGTDVTKATHQEAVMELLRPGDQIVLTVQHDPLPENYQLVEIEYIPVTELVITKEPGEKLGMHIKGGLRGQKGNPLDHTDEGVFISKINSGGAAKRDGRLKVGMRLLEVNGTSLLGATHQEAVNILRCSGNTITLVVCKGYDKSEIEPVLPISDGRDSKESRTSKELKDATTEGTKSLSQSISSLDRDDEEAATLRQEQEMKAELVAWEQEERERALLEHREKSTPEKVLDVVRAAESLVSKSNSPVDMVVPPKSPGGTKDLKTTTIVMSKHTLAPQNTNSLRKERTGTSVDNPSPKSPISTLTPSMNFDHTTAVQSLPSPKKKSSIPKRSITFADLPPISKEPINYSISAKEDPSPLHDSKFTFDPQTISYKKVYQNPIQATHSRFKLPPTPLTAPKSIEDYGTSISFGKRQNARSVDGNSQVELSPTTSPTPPLVKMSVSDKKKLFESAMEEHLKPSPKPEKVFSFLSQDEVEKMKQEEEKKIATLTRDELKSWAQLDENEGLEDLEETLEDQDNRRPNSRLSSRTSIPLLQNLPSSVRTAKAERRLKERLIQEGIISDEDEESHLSPAEQRALRAEKRAAWRQARLKSLEQDALQAQIVIKKMSEMMDTNKTSSMQDSTDIPITPVSESEKTTDFTTLRPSSADFPKLAVRSKVGPPKEIRESEKVVDEKVTRRTEEYVDEVTGERRVRTVEYVEKLIERQVETLREKIISLELSNAEDEIESITGTGASDAESESEEITVQPPTMTTLQERNETIISTNEGAAPKISANTVLVSKKKKRKRSKKGRH

Target ORF information:

RefSeq Version	XM_017065896.1
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana protein lap4 (LOC108003564), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017065896.1

ORF Insert Sequence:

ATGTCTCTTA CGAATCTACC GCCTGATTTC GGAAGCTTAG AGGCGTTACA ATCATTGGAG 
TTAAGGGAAA ATTTGCTCAA GTCCTTGCCA GAATCGCTAT CCCAACTTTA TAAATTGGAG 
CGGCTAGATC TTGGTGACAA TGACATTGAA GTTTTACCAG CACATATAGG AGAATTACCA 
GCATTACAAG AATTATGGCT GGATCATAAT CAGTTACAAC ATTTACCACC AGAAATAGGA 
GAATTGAAAA CGTTAGTGTG TCTGGATGTA TCTGAAAATC GTTTGGAAGA TCTCCCAGAA 
GAAATAGGAG GTCTAGAGGC TTTGACGGAT CTTCATTTAT CGCAAAATGT TATTGAAAAA 
TTACCTGATG GGCTTGGAGA ATTAAAGAAA CTTACTATTC TTAAAGTCGA TCAAAATAGG 
CTTTCTACTC TAAATCCAAA TATAGGCAGG TGTGAAAATT TACAAGAATT AATTCTTACG 
GAAAATTTTC TCTTAGAACT GCCTGTATCT ATAGGAAAAC TTCTTAATCT AAATAATTTG 
AATGTAGATA GGAATAGTTT GCAATCACTT CCTACGGAAA CGGGAAATTT AAAACAATTG 
GGTGTACTGT CCTTAAGAGA TAATAAATTA CAATATCTTC CAATCGAAGT TGGACAATGT 
ACTGCTCTCC ATGTATTGGA TGTGTCTGGC AATAGATTAC AATACTTGCC ATATTCCTTA 
ATCAATTTGA ACTTGAAGGC AGTATGGTTA AGTAAAAATC AAGCACAACC AATGCTTACT 
TTTCAGACAG ATGTTGATGA AGAAACTGGA CAAGAAGTAT TAACCTGTTT TCTTTTACCA 
CAATTAGAAA TTCATCCAGA TGATTCAGGA AGACTTGGTA TGCTTGCTGG AATGAGAAAT 
GCTGTTCAAG ATGGTGAAAT ACGTGAACTT GGTAGTAGTG ATGATGAAGG ATGGCAAGAA 
AAAGAAGCAT CAAGAACGCA TTCTGTCAAG TTTACAGATG AACCTCCAGA AACTGATAAG 
GAGACACCTT TTGTACGACA AAATACACCT CATCCCAAAG AATTAAAAGC AAAAGCTCAT 
AAATTATTTA GTAAAGGAAA AAATGACAGT CGTTCAGCAT CTACAGATGA GCAAGATGTT 
TCTCAAACAT TTGGTTTGGA CAAAACTGAA ACTGATGTTT CAACAAAACC TAACGATTTG 
ACAGTTGATT TGATAGAACA AGAATCATTA AAACATGAAT CTGTAGAAAA TGAACAAAAA 
GAAATTGTAC CTGGAGTAAC TGATAATATA GATGTTCAAT CTAGTAATGA ACCAGAAATA 
GTTTCTAATC AATATATAGT AGAATCTAAT ACTGACGTAA GAACTGAAGG TTCAATATCT 
GAGTTAAAGG ATAGAAATGA TAAGGAGGAT GATGAATCTG AAAATGAAGA AACACAAAGA 
CATGTAGAAT TTTCTATAGC TGAAGGTACA GATTATGATG GTAGTGGTGA TTCAAATAGA 
CCAAATAGAC TTCATCGTAG AGATACTCCA CATCATTTGA AAAACAAGCG AATTCATACA 
GCAGTGGATA AAGAAAAAGT AGCATCCATT ATTGCACAAG CACTCATGAA AAAAAATGAT 
GAAATTTCCA CATCCACATC AGCACCTGCA GAAACTACAG AAAATTTTGA TGCTCAAAGT 
CAAGATGCGA TTTCAGATGC TGAGCCGACA ATAGAAGTAC GAGAAGAACA ATACGAAATT 
CACATCGAAA GAACAACAGG TGGTCTAGGC TTATCAATAG CTGGTGGTAT TGGTTCAACC 
CCTTTTAAAG GCGATGATGA AGGCATTTTT ATTTCCAGAG TTACTGAAGG TGGACCTGCA 
GATTTAGCAG GTTTAAAAGT GGAAGATAAA GTATTATCTG TTAATGGTGT ATCAGTAGTA 
AATGTAGGTC ATTATGATGC TGTAGAAGTT TTGAAAGCTT GTGGACGAGT TCTTGTATTA 
GTAGTTCAAA GAGAAGTTAC AAGGATAGTG CCTCCATTTG AACAGCAGAT ATCATCGAGA 
AAAAACTCAG TGTGCTCTAG TTTAAGTACT AGTAGAGCTC CAAGTGCAAC ATCTCATGTT 
TCATCTATAG GTTTTTCACA TAATTTAGAA AATGGTGATG CTTTATCTCA TGATGCTATT 
AAGTGTAAGA AAATTCCTGA ACCTATATCT TCAACAAAAG TAGGCAATGA ACCAATGGTA 
TCAGTTCTTG TTCACACAAC ATTAATACGG GACCAAAATG GGCTTGGATT TAGTATTGCT 
GGTGGAGAAG GTTCTCCACC ATTCAAAGAC AATAGTGATG CGATATATAT TTCAAGAATA 
ACAGATGGCG GCGTAGCACA AAAAGATGGT AAATTATTAG TTGGAGACAA AGTAATATCT 
ATTAATGGAG TTGAGATGAG AGGAGCTAAG CATGAACAAG CAGTTGCTTT ATTAACAGGT 
TTAGAAAGAT TTGTTCGATT AGTAGTTGAA CGCGAAATAC CATTTTCGCA AGCAAATGCA 
GCTACGGTCG TAACACCTTC AGAAAAATCA CCACGAGTGA TCGGTGCACC TAAACCATAT 
ACAGGATTAT ATAATGCCAA TAGTTATATA GCAAATAAAC CGGGATATAC TGGATACAGA 
CGTTCTATAG ATGCTGATAG AACATTATCT CCAAGCCCTA CTTCGAAAAC GCCTCCACCT 
ATGAAAATTG AAACTACTGA ATTACCGAAA ATGAACGGTG TTACAGAATC AGGAAATATT 
AAAAATGTTT CTTCGATATC ACCAAGTCCA ACAAATAGCC AACTACATCC ACAGCCTGCT 
CCTAGGCACA GCATTTCGCA ACCAACAATT ACACCTACAA CTACTACGAG TTCAACGCCC 
ACATCTTCTA CAGAACCTAG GTCAACCTCA CCCCAGGAAG ATATACAGGT ACCCAAGCCT 
ATCACCAATG AAGAATTTCA GGCTATGATT CCTGCTCATT TCCTTCGTCC TCCTACTTCC 
TCACCATCGC CAGATTCCCA TCAAGGCCCT ATCGTGACAG TGACAATAAA ACAGCCTGAT 
AATCTACCTG GAGATGTTAA TTTTCCTCCG GCTCCTACAA CACTTGGTAA AGTTACTGAG 
ACCATCACAA AGAGCACTCT CACCGAGACC GTCGTAACTA GGGTGACTGA AAATCAATTG 
GTACCACCAG TAATTATTGA GGACGTGATT CTTGTAAAAG AGGGATCTCT AGGATTTAGT 
ATTATTGGTG GTACGGATCA TTCATGCACT CCATTTGGTG CAAAGGAACC TGGAATATTT 
ATATCTCATG TCGTGCCTGG TGGTATAGCT GCAAAATCTG GTAAATTAAG AATGGGTGAT 
AGAATACTAA AGGTAAATGG TACTGATGTA ACAAAAGCTA CTCATCAAGA GGCTGTGATG 
GAACTTTTGC GACCAGGAGA TCAAATAGTG CTTACTGTCC AACACGATCC ACTACCCGAA 
AATTATCAGC TGGTCGAAAT AGAGTACATC CCTGTGACAG AATTAGTTAT TACAAAAGAA 
CCAGGAGAGA AACTGGGCAT GCATATTAAA GGTGGACTTC GAGGACAAAA AGGTAATCCA 
CTTGATCATA CAGATGAAGG GGTTTTTATA TCCAAAATCA ACTCAGGTGG TGCAGCTAAA 
AGAGATGGAA GACTGAAAGT TGGAATGAGA CTATTAGAAG TTAATGGTAC ATCATTATTA 
GGCGCAACTC ACCAAGAAGC AGTAAATATA TTGCGATGTT CAGGAAATAC GATTACTTTG 
GTGGTTTGTA AAGGATATGA TAAGAGTGAA ATTGAACCAG TATTACCAAT ATCCGATGGA 
AGAGATTCTA AGGAATCTAG AACATCCAAA GAATTAAAGG ATGCTACAAC AGAAGGTACT 
AAATCGTTAT CACAAAGTAT ATCCAGTCTG GATCGCGATG ATGAAGAAGC AGCAACTCTT 
AGACAAGAAC AGGAAATGAA AGCTGAACTT GTCGCATGGG AACAAGAAGA AAGAGAACGT 
GCTTTGCTTG AGCATAGAGA AAAATCTACT CCTGAAAAAG TATTAGATGT AGTAAGAGCA 
GCTGAATCAT TAGTAAGCAA ATCAAATAGT CCGGTTGATA TGGTCGTACC ACCAAAGTCA 
CCAGGTGGTA CGAAAGATCT CAAAACAACC ACTATTGTGA TGAGCAAACA TACTCTAGCA 
CCTCAAAATA CAAATTCACT GAGGAAAGAG AGAACTGGAA CATCAGTGGA CAATCCATCG 
CCCAAGTCTC CGATCTCTAC TTTAACTCCA TCGATGAATT TCGATCACAC TACTGCTGTC 
CAAAGCTTGC CCTCTCCAAA GAAAAAAAGT TCCATACCAA AACGTAGTAT TACATTTGCT 
GATCTTCCTC CCATTTCTAA AGAACCAATT AATTATTCCA TTTCGGCGAA AGAAGATCCA 
TCACCTTTGC ATGATAGTAA ATTTACTTTC GATCCTCAAA CTATTTCTTA TAAGAAAGTC 
TATCAAAATC CTATCCAAGC TACTCATTCT CGTTTCAAAC TTCCTCCTAC ACCTTTAACT 
GCTCCTAAAT CTATAGAAGA TTATGGTACT TCAATTTCTT TTGGTAAACG ACAGAACGCA 
CGCTCTGTTG ATGGGAATAG TCAGGTTGAG CTTTCTCCAA CAACATCACC AACACCACCT 
CTAGTAAAGA TGTCGGTTAG TGATAAAAAA AAATTGTTTG AAAGTGCCAT GGAGGAACAT 
TTAAAACCTT CCCCTAAACC AGAAAAAGTA TTCAGTTTTC TAAGTCAGGA TGAAGTTGAG 
AAAATGAAAC AAGAAGAAGA AAAGAAGATT GCTACTCTAA CGCGGGATGA ATTAAAGTCG 
TGGGCACAAC TTGATGAAAA TGAAGGTTTG GAAGATTTAG AAGAAACATT AGAAGATCAG 
GATAATCGGC GACCTAATAG CCGACTGAGT TCGCGAACTT CGATCCCTCT TCTGCAAAAT 
CTTCCAAGCA GTGTAAGGAC AGCAAAAGCA GAACGCCGCT TGAAAGAAAG ATTAATACAG 
GAGGGTATAA TTTCGGATGA AGATGAAGAA AGTCATTTAA GTCCTGCCGA ACAGAGAGCG 
TTAAGAGCAG AAAAACGTGC GGCATGGAGA CAAGCACGTT TGAAATCTCT GGAACAAGAT 
GCTCTTCAAG CACAGATAGT AATTAAAAAA ATGAGTGAAA TGATGGACAC GAATAAAACA 
AGTTCAATGC AGGATTCTAC AGATATTCCT ATTACTCCTG TTTCTGAATC GGAAAAGACA 
ACTGATTTCA CTACGTTACG GCCGTCTAGT GCAGATTTTC CCAAACTTGC TGTACGTAGC 
AAAGTGGGTC CCCCTAAAGA GATACGCGAA TCCGAGAAAG TAGTGGACGA GAAGGTCACT 
CGACGTACTG AGGAATATGT GGATGAGGTG ACGGGTGAAC GACGTGTGCG AACAGTCGAA 
TACGTCGAGA AACTCATTGA ACGACAGGTT GAAACTCTAC GAGAAAAGAT AATATCATTG 
GAATTAAGTA ATGCAGAAGA TGAAATAGAA AGTATTACCG GTACAGGAGC AAGTGATGCT 
GAAAGCGAAA GCGAAGAAAT TACTGTACAA CCTCCTACTA TGACTACTTT ACAAGAAAGA 
AATGAAACAA TTATATCTAC AAATGAAGGT GCCGCACCTA AAATAAGTGC AAATACTGTT 
CTTGTTTCAA AAAAAAAGAA ACGAAAACGT TCGAAGAAAG GTCGTCATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108003564 ( XM_017065897.1 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_016921386.1 Apis cerana protein lap4 (LOC108003564), transcript variant X11, mRNA.

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CloneID	OAp29730
Clone ID Related Accession (Same CDS sequence)	XM_017065897.1 , XM_017065897.2
Accession Version	XM_017065897.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-08
Organism	Apis cerana(Asiatic honeybee)
Product	protein lap4 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016017567.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis cerana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGC AGATATCATC GAGAAAAAAC 
TCAGTGTGCT CTAGTTTAAG TACTAGTAGA GCTCCAAGTG CAACATCTCA TGTTTCATCT 
ATAGGTTTTT CACATAATTT AGAAAATGGT GATGCTTTAT CTCATGATGC TATTAAGTGT 
AAGAAAATTC CTGAACCTAT ATCTTCAACA AAAGTAGGCA ATGAACCAAT GGTATCAGTT 
CTTGTTCACA CAACATTAAT ACGGGACCAA AATGGGCTTG GATTTAGTAT TGCTGGTGGA 
GAAGGTTCTC CACCATTCAA AGACAATAGT GATGCGATAT ATATTTCAAG AATAACAGAT 
GGCGGCGTAG CACAAAAAGA TGGTAAATTA TTAGTTGGAG ACAAAGTAAT ATCTATTAAT 
GGAGTTGAGA TGAGAGGAGC TAAGCATGAA CAAGCAGTTG CTTTATTAAC AGGTTTAGAA 
AGATTTGTTC GATTAGTAGT TGAACGCGAA ATACCATTTT CGCAAGCAAA TGCAGCTACG 
GTCGTAACAC CTTCAGAAAA ATCACCACGA GTGATCGGTG CACCTAAACC ATATACAGGA 
TTATATAATG CCAATAGTTA TATAGCAAAT AAACCGGGAT ATACTGGATA CAGACGTTCT 
ATAGATGCTG ATAGAACATT ATCTCCAAGC CCTACTTCGA AAACGCCTCC ACCTATGAAA 
ATTGAAACTA CTGAATTACC GAAAATGAAC GGTGTTACAG AATCAGGAAA TATTAAAAAT 
GTTTCTTCGA TATCACCAAG TCCAACAAAT AGCCAACTAC ATCCACAGCC TGCTCCTAGG 
CACAGCATTT CGCAACCAAC AATTACACCT ACAACTACTA CGAGTTCAAC GCCCACATCT 
TCTACAGAAC CTAGGTCAAC CTCACCCCAG GAAGATATAC AGGTACCCAA GCCTATCACC 
AATGAAGAAT TTCAGGCTAT GATTCCTGCT CATTTCCTTC GTCCTCCTAC TTCCTCACCA 
TCGCCAGATT CCCATCAAGG CCCTATCGTG ACAGTGACAA TAAAACAGCC TGATAATCTA 
CCTGGAGATG TTAATTTTCC TCCGGCTCCT ACAACACTTG GTAAAGTTAC TGAGACCATC 
ACAAAGAGCA CTCTCACCGA GACCGTCGTA ACTAGGGTGA CTGAAAATCA ATTGGTACCA 
CCAGTAATTA TTGAGGACGT GATTCTTGTA AAAGAGGGAT CTCTAGGATT TAGTATTATT 
GGTGGTACGG ATCATTCATG CACTCCATTT GGTGCAAAGG AACCTGGAAT ATTTATATCT 
CATGTCGTGC CTGGTGGTAT AGCTGCAAAA TCTGGTAAAT TAAGAATGGG TGATAGAATA 
CTAAAGGTAA ATGGTACTGA TGTAACAAAA GCTACTCATC AAGAGGCTGT GATGGAACTT 
TTGCGACCAG GAGATCAAAT AGTGCTTACT GTCCAACACG ATCCACTACC CGAAAATTAT 
CAGCTGGTCG AAATAGAGTA CATCCCTGTG ACAGAATTAG TTATTACAAA AGAACCAGGA 
GAGAAACTGG GCATGCATAT TAAAGGTGGA CTTCGAGGAC AAAAAGGTAA TCCACTTGAT 
CATACAGATG AAGGGGTTTT TATATCCAAA ATCAACTCAG GTGGTGCAGC TAAAAGAGAT 
GGAAGACTGA AAGTTGGAAT GAGACTATTA GAAGTTAATG GTACATCATT ATTAGGCGCA 
ACTCACCAAG AAGCAGTAAA TATATTGCGA TGTTCAGGAA ATACGATTAC TTTGGTGGTT 
TGTAAAGGAT ATGATAAGAG TGAAATTGAA CCAGTATTAC CAATATCCGA TGGAAGAGAT 
TCTAAGGAAT CTAGAACATC CAAAGAATTA AAGGATGCTA CAACAGAAGG TACTAAATCG 
TTATCACAAA GTATATCCAG TCTGGATCGC GATGATGAAG AAGCAGCAAC TCTTAGACAA 
GAACAGGAAA TGAAAGCTGA ACTTGTCGCA TGGGAACAAG AAGAAAGAGA ACGTGCTTTG 
CTTGAGCATA GAGAAAAATC TACTCCTGAA AAAGTATTAG ATGTAGTAAG AGCAGCTGAA 
TCATTAGTAA GCAAATCAAA TAGTCCGGTT GATATGGTCG TACCACCAAA GTCACCAGGT 
GGTACGAAAG ATCTCAAAAC AACCACTATT GTGATGAGCA AACATACTCT AGCACCTCAA 
AATACAAATC TTTCTCCAAC AACATCACCA ACACCACCTC TAGTAAAGAT GTCGGTTAGT 
GATAAAAAAA AATTGTTTGA AAGTGCCATG GAGGAACATT TAAAACCTTC CCCTAAACCA 
GAAAAAGTAT TCAGTTTTCT AAGTCAGGAT GAAGTTGAGA AAATGAAACA AGAAGAAGAA 
AAGAAGATTG CTACTCTAAC GCGGGATGAA TTAAAGTCGT GGGCACAACT TGATGAAAAT 
GAAGGTTTGG AAGATTTAGA AGAAACATTA GAAGATCAGG ATAATCGGCG ACCTAATAGC 
CGACTGAGTT CGCGAACTTC GATCCCTCTT CTGCAAAATC TTCCAAGCAG TGTAAGGACA 
GCAAAAGCAG AACGCCGCTT GAAAGAAAGA TTAATACAGG AGGGTATAAT TTCGGATGAA 
GATGAAGAAA GTCATTTAAG TCCTGCCGAA CAGAGAGCGT TAAGAGCAGA AAAACGTGCG 
GCATGGAGAC AAGCACGTTT GAAATCTCTG GAACAAGATG CTCTTCAAGC ACAGATAGTA 
ATTAAAAAAA TGAGTGAAAT GATGGACACG AATAAAACAA GTTCAATGCA GGATTCTACA 
GATATTCCTA TTACTCCTGT TTCTGAATCG GAAAAGACAA CTGATTTCAC TACGTTACGG 
CCGTCTAGTG CAGATTTTCC CAAACTTGCT GTACGTAGCA AAGTGGGTCC CCCTAAAGAG 
ATACGCGAAT CCGAGAAAGT AGTGGACGAG AAGGTCACTC GACGTACTGA GGAATATGTG 
GATGAGGTGA CGGGTGAACG ACGTGTGCGA ACAGTCGAAT ACGTCGAGAA ACTCATTGAA 
CGACAGGTTG AAACTCTACG AGAAAAGATA ATATCATTGG AATTAAGTAA TGCAGAAGAT 
GAAATAGAAA GTATTACCGG TACAGGAGCA AGTGATGCTG AAAGCGAAAG CGAAGAAATT 
ACTGTACAAC CTCCTACTAT GACTACTTTA CAAGAAAGAA ATGAAACAAT TATATCTACA 
AATGAAGGTG CCGCACCTAA AATAAGTGCA AATACTGTTC TTGTTTCAAA AAAAAAGAAA 
CGAAAACGTT CGAAGAAAGG TCGTCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016921386.1
CDS	233..5962
Translation	MFRCIPIFKGCNRQVEYIDKRHCSLPCVPDDILRYSRSLEELLLDANHIRDLPKNFFRLQKLRKLGLSDNEIHRLPPDIQNFENLVELDVSRNDIPDIPENIKNLRALQVADFSSNPIPRLPAGFVQLRNLTVLGLNDMSLTNLPPDFGSLEALQSLELRENLLKSLPESLSQLYKLERLDLGDNDIEVLPAHIGELPALQELWLDHNQLQHLPPEIGELKTLVCLDVSENRLEDLPEEIGGLEALTDLHLSQNVIEKLPDGLGELKKLTILKVDQNRLSTLNPNIGRCENLQELILTENFLLELPVSIGKLLNLNNLNVDRNSLQSLPTETGNLKQLGVLSLRDNKLQYLPIEVGQCTALHVLDVSGNRLQYLPYSLINLNLKAVWLSKNQAQPMLTFQTDVDEETGQEVLTCFLLPQLEIHPDDSGRLGMLAGMRNAVQDGEIRELGSSDDEGWQEKEASRTHSVKFTDEPPETDKETPFVRQNTPHPKELKAKAHKLFSKGKNDSRSASTDEQDVSQTFGLDKTETDVSTKPNDLTVDLIEQESLKHESVENEQKEIVPGVTDNIDVQSSNEPEIVSNQYIVESNTDVRTEGSISELKDRNDKEDDESENEETQRHVEFSIAEGTDYDGSGDSNRPNRLHRRDTPHHLKNKRIHTAVDKEKVASIIAQALMKKNDEISTSTSAPAETTENFDAQSQDAISDAEPTIEVREEQYEIHIERTTGGLGLSIAGGIGSTPFKGDDEGIFISRVTEGGPADLAGLKVEDKVLSVNGVSVVNVGHYDAVEVLKACGRVLVLVVQREVTRIVPPFEQQISSRKNSVCSSLSTSRAPSATSHVSSIGFSHNLENGDALSHDAIKCKKIPEPISSTKVGNEPMVSVLVHTTLIRDQNGLGFSIAGGEGSPPFKDNSDAIYISRITDGGVAQKDGKLLVGDKVISINGVEMRGAKHEQAVALLTGLERFVRLVVEREIPFSQANAATVVTPSEKSPRVIGAPKPYTGLYNANSYIANKPGYTGYRRSIDADRTLSPSPTSKTPPPMKIETTELPKMNGVTESGNIKNVSSISPSPTNSQLHPQPAPRHSISQPTITPTTTTSSTPTSSTEPRSTSPQEDIQVPKPITNEEFQAMIPAHFLRPPTSSPSPDSHQGPIVTVTIKQPDNLPGDVNFPPAPTTLGKVTETITKSTLTETVVTRVTENQLVPPVIIEDVILVKEGSLGFSIIGGTDHSCTPFGAKEPGIFISHVVPGGIAAKSGKLRMGDRILKVNGTDVTKATHQEAVMELLRPGDQIVLTVQHDPLPENYQLVEIEYIPVTELVITKEPGEKLGMHIKGGLRGQKGNPLDHTDEGVFISKINSGGAAKRDGRLKVGMRLLEVNGTSLLGATHQEAVNILRCSGNTITLVVCKGYDKSEIEPVLPISDGRDSKESRTSKELKDATTEGTKSLSQSISSLDRDDEEAATLRQEQEMKAELVAWEQEERERALLEHREKSTPEKVLDVVRAAESLVSKSNSPVDMVVPPKSPGGTKDLKTTTIVMSKHTLAPQNTNLSPTTSPTPPLVKMSVSDKKKLFESAMEEHLKPSPKPEKVFSFLSQDEVEKMKQEEEKKIATLTRDELKSWAQLDENEGLEDLEETLEDQDNRRPNSRLSSRTSIPLLQNLPSSVRTAKAERRLKERLIQEGIISDEDEESHLSPAEQRALRAEKRAAWRQARLKSLEQDALQAQIVIKKMSEMMDTNKTSSMQDSTDIPITPVSESEKTTDFTTLRPSSADFPKLAVRSKVGPPKEIRESEKVVDEKVTRRTEEYVDEVTGERRVRTVEYVEKLIERQVETLREKIISLELSNAEDEIESITGTGASDAESESEEITVQPPTMTTLQERNETIISTNEGAAPKISANTVLVSKKKKRKRSKKGRH

Target ORF information:

RefSeq Version	XM_017065897.1
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana protein lap4 (LOC108003564), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017065897.1

ORF Insert Sequence:

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGC AGATATCATC GAGAAAAAAC 
TCAGTGTGCT CTAGTTTAAG TACTAGTAGA GCTCCAAGTG CAACATCTCA TGTTTCATCT 
ATAGGTTTTT CACATAATTT AGAAAATGGT GATGCTTTAT CTCATGATGC TATTAAGTGT 
AAGAAAATTC CTGAACCTAT ATCTTCAACA AAAGTAGGCA ATGAACCAAT GGTATCAGTT 
CTTGTTCACA CAACATTAAT ACGGGACCAA AATGGGCTTG GATTTAGTAT TGCTGGTGGA 
GAAGGTTCTC CACCATTCAA AGACAATAGT GATGCGATAT ATATTTCAAG AATAACAGAT 
GGCGGCGTAG CACAAAAAGA TGGTAAATTA TTAGTTGGAG ACAAAGTAAT ATCTATTAAT 
GGAGTTGAGA TGAGAGGAGC TAAGCATGAA CAAGCAGTTG CTTTATTAAC AGGTTTAGAA 
AGATTTGTTC GATTAGTAGT TGAACGCGAA ATACCATTTT CGCAAGCAAA TGCAGCTACG 
GTCGTAACAC CTTCAGAAAA ATCACCACGA GTGATCGGTG CACCTAAACC ATATACAGGA 
TTATATAATG CCAATAGTTA TATAGCAAAT AAACCGGGAT ATACTGGATA CAGACGTTCT 
ATAGATGCTG ATAGAACATT ATCTCCAAGC CCTACTTCGA AAACGCCTCC ACCTATGAAA 
ATTGAAACTA CTGAATTACC GAAAATGAAC GGTGTTACAG AATCAGGAAA TATTAAAAAT 
GTTTCTTCGA TATCACCAAG TCCAACAAAT AGCCAACTAC ATCCACAGCC TGCTCCTAGG 
CACAGCATTT CGCAACCAAC AATTACACCT ACAACTACTA CGAGTTCAAC GCCCACATCT 
TCTACAGAAC CTAGGTCAAC CTCACCCCAG GAAGATATAC AGGTACCCAA GCCTATCACC 
AATGAAGAAT TTCAGGCTAT GATTCCTGCT CATTTCCTTC GTCCTCCTAC TTCCTCACCA 
TCGCCAGATT CCCATCAAGG CCCTATCGTG ACAGTGACAA TAAAACAGCC TGATAATCTA 
CCTGGAGATG TTAATTTTCC TCCGGCTCCT ACAACACTTG GTAAAGTTAC TGAGACCATC 
ACAAAGAGCA CTCTCACCGA GACCGTCGTA ACTAGGGTGA CTGAAAATCA ATTGGTACCA 
CCAGTAATTA TTGAGGACGT GATTCTTGTA AAAGAGGGAT CTCTAGGATT TAGTATTATT 
GGTGGTACGG ATCATTCATG CACTCCATTT GGTGCAAAGG AACCTGGAAT ATTTATATCT 
CATGTCGTGC CTGGTGGTAT AGCTGCAAAA TCTGGTAAAT TAAGAATGGG TGATAGAATA 
CTAAAGGTAA ATGGTACTGA TGTAACAAAA GCTACTCATC AAGAGGCTGT GATGGAACTT 
TTGCGACCAG GAGATCAAAT AGTGCTTACT GTCCAACACG ATCCACTACC CGAAAATTAT 
CAGCTGGTCG AAATAGAGTA CATCCCTGTG ACAGAATTAG TTATTACAAA AGAACCAGGA 
GAGAAACTGG GCATGCATAT TAAAGGTGGA CTTCGAGGAC AAAAAGGTAA TCCACTTGAT 
CATACAGATG AAGGGGTTTT TATATCCAAA ATCAACTCAG GTGGTGCAGC TAAAAGAGAT 
GGAAGACTGA AAGTTGGAAT GAGACTATTA GAAGTTAATG GTACATCATT ATTAGGCGCA 
ACTCACCAAG AAGCAGTAAA TATATTGCGA TGTTCAGGAA ATACGATTAC TTTGGTGGTT 
TGTAAAGGAT ATGATAAGAG TGAAATTGAA CCAGTATTAC CAATATCCGA TGGAAGAGAT 
TCTAAGGAAT CTAGAACATC CAAAGAATTA AAGGATGCTA CAACAGAAGG TACTAAATCG 
TTATCACAAA GTATATCCAG TCTGGATCGC GATGATGAAG AAGCAGCAAC TCTTAGACAA 
GAACAGGAAA TGAAAGCTGA ACTTGTCGCA TGGGAACAAG AAGAAAGAGA ACGTGCTTTG 
CTTGAGCATA GAGAAAAATC TACTCCTGAA AAAGTATTAG ATGTAGTAAG AGCAGCTGAA 
TCATTAGTAA GCAAATCAAA TAGTCCGGTT GATATGGTCG TACCACCAAA GTCACCAGGT 
GGTACGAAAG ATCTCAAAAC AACCACTATT GTGATGAGCA AACATACTCT AGCACCTCAA 
AATACAAATC TTTCTCCAAC AACATCACCA ACACCACCTC TAGTAAAGAT GTCGGTTAGT 
GATAAAAAAA AATTGTTTGA AAGTGCCATG GAGGAACATT TAAAACCTTC CCCTAAACCA 
GAAAAAGTAT TCAGTTTTCT AAGTCAGGAT GAAGTTGAGA AAATGAAACA AGAAGAAGAA 
AAGAAGATTG CTACTCTAAC GCGGGATGAA TTAAAGTCGT GGGCACAACT TGATGAAAAT 
GAAGGTTTGG AAGATTTAGA AGAAACATTA GAAGATCAGG ATAATCGGCG ACCTAATAGC 
CGACTGAGTT CGCGAACTTC GATCCCTCTT CTGCAAAATC TTCCAAGCAG TGTAAGGACA 
GCAAAAGCAG AACGCCGCTT GAAAGAAAGA TTAATACAGG AGGGTATAAT TTCGGATGAA 
GATGAAGAAA GTCATTTAAG TCCTGCCGAA CAGAGAGCGT TAAGAGCAGA AAAACGTGCG 
GCATGGAGAC AAGCACGTTT GAAATCTCTG GAACAAGATG CTCTTCAAGC ACAGATAGTA 
ATTAAAAAAA TGAGTGAAAT GATGGACACG AATAAAACAA GTTCAATGCA GGATTCTACA 
GATATTCCTA TTACTCCTGT TTCTGAATCG GAAAAGACAA CTGATTTCAC TACGTTACGG 
CCGTCTAGTG CAGATTTTCC CAAACTTGCT GTACGTAGCA AAGTGGGTCC CCCTAAAGAG 
ATACGCGAAT CCGAGAAAGT AGTGGACGAG AAGGTCACTC GACGTACTGA GGAATATGTG 
GATGAGGTGA CGGGTGAACG ACGTGTGCGA ACAGTCGAAT ACGTCGAGAA ACTCATTGAA 
CGACAGGTTG AAACTCTACG AGAAAAGATA ATATCATTGG AATTAAGTAA TGCAGAAGAT 
GAAATAGAAA GTATTACCGG TACAGGAGCA AGTGATGCTG AAAGCGAAAG CGAAGAAATT 
ACTGTACAAC CTCCTACTAT GACTACTTTA CAAGAAAGAA ATGAAACAAT TATATCTACA 
AATGAAGGTG CCGCACCTAA AATAAGTGCA AATACTGTTC TTGTTTCAAA AAAAAAGAAA 
CGAAAACGTT CGAAGAAAGG TCGTCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108003564 ( XM_017065897.2 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_016921386.1 Apis cerana protein lap4 (LOC108003564), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OAp29730
Clone ID Related Accession (Same CDS sequence)	XM_017065897.1 , XM_017065897.2
Accession Version	XM_017065897.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-16
Organism	Apis cerana(Asiatic honeybee)
Product	protein lap4 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016017567.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 17, 2019 this sequence version replaced XM_017065897.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis cerana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGC AGATATCATC GAGAAAAAAC 
TCAGTGTGCT CTAGTTTAAG TACTAGTAGA GCTCCAAGTG CAACATCTCA TGTTTCATCT 
ATAGGTTTTT CACATAATTT AGAAAATGGT GATGCTTTAT CTCATGATGC TATTAAGTGT 
AAGAAAATTC CTGAACCTAT ATCTTCAACA AAAGTAGGCA ATGAACCAAT GGTATCAGTT 
CTTGTTCACA CAACATTAAT ACGGGACCAA AATGGGCTTG GATTTAGTAT TGCTGGTGGA 
GAAGGTTCTC CACCATTCAA AGACAATAGT GATGCGATAT ATATTTCAAG AATAACAGAT 
GGCGGCGTAG CACAAAAAGA TGGTAAATTA TTAGTTGGAG ACAAAGTAAT ATCTATTAAT 
GGAGTTGAGA TGAGAGGAGC TAAGCATGAA CAAGCAGTTG CTTTATTAAC AGGTTTAGAA 
AGATTTGTTC GATTAGTAGT TGAACGCGAA ATACCATTTT CGCAAGCAAA TGCAGCTACG 
GTCGTAACAC CTTCAGAAAA ATCACCACGA GTGATCGGTG CACCTAAACC ATATACAGGA 
TTATATAATG CCAATAGTTA TATAGCAAAT AAACCGGGAT ATACTGGATA CAGACGTTCT 
ATAGATGCTG ATAGAACATT ATCTCCAAGC CCTACTTCGA AAACGCCTCC ACCTATGAAA 
ATTGAAACTA CTGAATTACC GAAAATGAAC GGTGTTACAG AATCAGGAAA TATTAAAAAT 
GTTTCTTCGA TATCACCAAG TCCAACAAAT AGCCAACTAC ATCCACAGCC TGCTCCTAGG 
CACAGCATTT CGCAACCAAC AATTACACCT ACAACTACTA CGAGTTCAAC GCCCACATCT 
TCTACAGAAC CTAGGTCAAC CTCACCCCAG GAAGATATAC AGGTACCCAA GCCTATCACC 
AATGAAGAAT TTCAGGCTAT GATTCCTGCT CATTTCCTTC GTCCTCCTAC TTCCTCACCA 
TCGCCAGATT CCCATCAAGG CCCTATCGTG ACAGTGACAA TAAAACAGCC TGATAATCTA 
CCTGGAGATG TTAATTTTCC TCCGGCTCCT ACAACACTTG GTAAAGTTAC TGAGACCATC 
ACAAAGAGCA CTCTCACCGA GACCGTCGTA ACTAGGGTGA CTGAAAATCA ATTGGTACCA 
CCAGTAATTA TTGAGGACGT GATTCTTGTA AAAGAGGGAT CTCTAGGATT TAGTATTATT 
GGTGGTACGG ATCATTCATG CACTCCATTT GGTGCAAAGG AACCTGGAAT ATTTATATCT 
CATGTCGTGC CTGGTGGTAT AGCTGCAAAA TCTGGTAAAT TAAGAATGGG TGATAGAATA 
CTAAAGGTAA ATGGTACTGA TGTAACAAAA GCTACTCATC AAGAGGCTGT GATGGAACTT 
TTGCGACCAG GAGATCAAAT AGTGCTTACT GTCCAACACG ATCCACTACC CGAAAATTAT 
CAGCTGGTCG AAATAGAGTA CATCCCTGTG ACAGAATTAG TTATTACAAA AGAACCAGGA 
GAGAAACTGG GCATGCATAT TAAAGGTGGA CTTCGAGGAC AAAAAGGTAA TCCACTTGAT 
CATACAGATG AAGGGGTTTT TATATCCAAA ATCAACTCAG GTGGTGCAGC TAAAAGAGAT 
GGAAGACTGA AAGTTGGAAT GAGACTATTA GAAGTTAATG GTACATCATT ATTAGGCGCA 
ACTCACCAAG AAGCAGTAAA TATATTGCGA TGTTCAGGAA ATACGATTAC TTTGGTGGTT 
TGTAAAGGAT ATGATAAGAG TGAAATTGAA CCAGTATTAC CAATATCCGA TGGAAGAGAT 
TCTAAGGAAT CTAGAACATC CAAAGAATTA AAGGATGCTA CAACAGAAGG TACTAAATCG 
TTATCACAAA GTATATCCAG TCTGGATCGC GATGATGAAG AAGCAGCAAC TCTTAGACAA 
GAACAGGAAA TGAAAGCTGA ACTTGTCGCA TGGGAACAAG AAGAAAGAGA ACGTGCTTTG 
CTTGAGCATA GAGAAAAATC TACTCCTGAA AAAGTATTAG ATGTAGTAAG AGCAGCTGAA 
TCATTAGTAA GCAAATCAAA TAGTCCGGTT GATATGGTCG TACCACCAAA GTCACCAGGT 
GGTACGAAAG ATCTCAAAAC AACCACTATT GTGATGAGCA AACATACTCT AGCACCTCAA 
AATACAAATC TTTCTCCAAC AACATCACCA ACACCACCTC TAGTAAAGAT GTCGGTTAGT 
GATAAAAAAA AATTGTTTGA AAGTGCCATG GAGGAACATT TAAAACCTTC CCCTAAACCA 
GAAAAAGTAT TCAGTTTTCT AAGTCAGGAT GAAGTTGAGA AAATGAAACA AGAAGAAGAA 
AAGAAGATTG CTACTCTAAC GCGGGATGAA TTAAAGTCGT GGGCACAACT TGATGAAAAT 
GAAGGTTTGG AAGATTTAGA AGAAACATTA GAAGATCAGG ATAATCGGCG ACCTAATAGC 
CGACTGAGTT CGCGAACTTC GATCCCTCTT CTGCAAAATC TTCCAAGCAG TGTAAGGACA 
GCAAAAGCAG AACGCCGCTT GAAAGAAAGA TTAATACAGG AGGGTATAAT TTCGGATGAA 
GATGAAGAAA GTCATTTAAG TCCTGCCGAA CAGAGAGCGT TAAGAGCAGA AAAACGTGCG 
GCATGGAGAC AAGCACGTTT GAAATCTCTG GAACAAGATG CTCTTCAAGC ACAGATAGTA 
ATTAAAAAAA TGAGTGAAAT GATGGACACG AATAAAACAA GTTCAATGCA GGATTCTACA 
GATATTCCTA TTACTCCTGT TTCTGAATCG GAAAAGACAA CTGATTTCAC TACGTTACGG 
CCGTCTAGTG CAGATTTTCC CAAACTTGCT GTACGTAGCA AAGTGGGTCC CCCTAAAGAG 
ATACGCGAAT CCGAGAAAGT AGTGGACGAG AAGGTCACTC GACGTACTGA GGAATATGTG 
GATGAGGTGA CGGGTGAACG ACGTGTGCGA ACAGTCGAAT ACGTCGAGAA ACTCATTGAA 
CGACAGGTTG AAACTCTACG AGAAAAGATA ATATCATTGG AATTAAGTAA TGCAGAAGAT 
GAAATAGAAA GTATTACCGG TACAGGAGCA AGTGATGCTG AAAGCGAAAG CGAAGAAATT 
ACTGTACAAC CTCCTACTAT GACTACTTTA CAAGAAAGAA ATGAAACAAT TATATCTACA 
AATGAAGGTG CCGCACCTAA AATAAGTGCA AATACTGTTC TTGTTTCAAA AAAAAAGAAA 
CGAAAACGTT CGAAGAAAGG TCGTCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016921386.1
CDS	1475..7204
Translation	MFRCIPIFKGCNRQVEYIDKRHCSLPCVPDDILRYSRSLEELLLDANHIRDLPKNFFRLQKLRKLGLSDNEIHRLPPDIQNFENLVELDVSRNDIPDIPENIKNLRALQVADFSSNPIPRLPAGFVQLRNLTVLGLNDMSLTNLPPDFGSLEALQSLELRENLLKSLPESLSQLYKLERLDLGDNDIEVLPAHIGELPALQELWLDHNQLQHLPPEIGELKTLVCLDVSENRLEDLPEEIGGLEALTDLHLSQNVIEKLPDGLGELKKLTILKVDQNRLSTLNPNIGRCENLQELILTENFLLELPVSIGKLLNLNNLNVDRNSLQSLPTETGNLKQLGVLSLRDNKLQYLPIEVGQCTALHVLDVSGNRLQYLPYSLINLNLKAVWLSKNQAQPMLTFQTDVDEETGQEVLTCFLLPQLEIHPDDSGRLGMLAGMRNAVQDGEIRELGSSDDEGWQEKEASRTHSVKFTDEPPETDKETPFVRQNTPHPKELKAKAHKLFSKGKNDSRSASTDEQDVSQTFGLDKTETDVSTKPNDLTVDLIEQESLKHESVENEQKEIVPGVTDNIDVQSSNEPEIVSNQYIVESNTDVRTEGSISELKDRNDKEDDESENEETQRHVEFSIAEGTDYDGSGDSNRPNRLHRRDTPHHLKNKRIHTAVDKEKVASIIAQALMKKNDEISTSTSAPAETTENFDAQSQDAISDAEPTIEVREEQYEIHIERTTGGLGLSIAGGIGSTPFKGDDEGIFISRVTEGGPADLAGLKVEDKVLSVNGVSVVNVGHYDAVEVLKACGRVLVLVVQREVTRIVPPFEQQISSRKNSVCSSLSTSRAPSATSHVSSIGFSHNLENGDALSHDAIKCKKIPEPISSTKVGNEPMVSVLVHTTLIRDQNGLGFSIAGGEGSPPFKDNSDAIYISRITDGGVAQKDGKLLVGDKVISINGVEMRGAKHEQAVALLTGLERFVRLVVEREIPFSQANAATVVTPSEKSPRVIGAPKPYTGLYNANSYIANKPGYTGYRRSIDADRTLSPSPTSKTPPPMKIETTELPKMNGVTESGNIKNVSSISPSPTNSQLHPQPAPRHSISQPTITPTTTTSSTPTSSTEPRSTSPQEDIQVPKPITNEEFQAMIPAHFLRPPTSSPSPDSHQGPIVTVTIKQPDNLPGDVNFPPAPTTLGKVTETITKSTLTETVVTRVTENQLVPPVIIEDVILVKEGSLGFSIIGGTDHSCTPFGAKEPGIFISHVVPGGIAAKSGKLRMGDRILKVNGTDVTKATHQEAVMELLRPGDQIVLTVQHDPLPENYQLVEIEYIPVTELVITKEPGEKLGMHIKGGLRGQKGNPLDHTDEGVFISKINSGGAAKRDGRLKVGMRLLEVNGTSLLGATHQEAVNILRCSGNTITLVVCKGYDKSEIEPVLPISDGRDSKESRTSKELKDATTEGTKSLSQSISSLDRDDEEAATLRQEQEMKAELVAWEQEERERALLEHREKSTPEKVLDVVRAAESLVSKSNSPVDMVVPPKSPGGTKDLKTTTIVMSKHTLAPQNTNLSPTTSPTPPLVKMSVSDKKKLFESAMEEHLKPSPKPEKVFSFLSQDEVEKMKQEEEKKIATLTRDELKSWAQLDENEGLEDLEETLEDQDNRRPNSRLSSRTSIPLLQNLPSSVRTAKAERRLKERLIQEGIISDEDEESHLSPAEQRALRAEKRAAWRQARLKSLEQDALQAQIVIKKMSEMMDTNKTSSMQDSTDIPITPVSESEKTTDFTTLRPSSADFPKLAVRSKVGPPKEIRESEKVVDEKVTRRTEEYVDEVTGERRVRTVEYVEKLIERQVETLREKIISLELSNAEDEIESITGTGASDAESESEEITVQPPTMTTLQERNETIISTNEGAAPKISANTVLVSKKKKRKRSKKGRH

Target ORF information:

RefSeq Version	XM_017065897.2
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana protein lap4 (LOC108003564), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017065897.2

ORF Insert Sequence:

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGC AGATATCATC GAGAAAAAAC 
TCAGTGTGCT CTAGTTTAAG TACTAGTAGA GCTCCAAGTG CAACATCTCA TGTTTCATCT 
ATAGGTTTTT CACATAATTT AGAAAATGGT GATGCTTTAT CTCATGATGC TATTAAGTGT 
AAGAAAATTC CTGAACCTAT ATCTTCAACA AAAGTAGGCA ATGAACCAAT GGTATCAGTT 
CTTGTTCACA CAACATTAAT ACGGGACCAA AATGGGCTTG GATTTAGTAT TGCTGGTGGA 
GAAGGTTCTC CACCATTCAA AGACAATAGT GATGCGATAT ATATTTCAAG AATAACAGAT 
GGCGGCGTAG CACAAAAAGA TGGTAAATTA TTAGTTGGAG ACAAAGTAAT ATCTATTAAT 
GGAGTTGAGA TGAGAGGAGC TAAGCATGAA CAAGCAGTTG CTTTATTAAC AGGTTTAGAA 
AGATTTGTTC GATTAGTAGT TGAACGCGAA ATACCATTTT CGCAAGCAAA TGCAGCTACG 
GTCGTAACAC CTTCAGAAAA ATCACCACGA GTGATCGGTG CACCTAAACC ATATACAGGA 
TTATATAATG CCAATAGTTA TATAGCAAAT AAACCGGGAT ATACTGGATA CAGACGTTCT 
ATAGATGCTG ATAGAACATT ATCTCCAAGC CCTACTTCGA AAACGCCTCC ACCTATGAAA 
ATTGAAACTA CTGAATTACC GAAAATGAAC GGTGTTACAG AATCAGGAAA TATTAAAAAT 
GTTTCTTCGA TATCACCAAG TCCAACAAAT AGCCAACTAC ATCCACAGCC TGCTCCTAGG 
CACAGCATTT CGCAACCAAC AATTACACCT ACAACTACTA CGAGTTCAAC GCCCACATCT 
TCTACAGAAC CTAGGTCAAC CTCACCCCAG GAAGATATAC AGGTACCCAA GCCTATCACC 
AATGAAGAAT TTCAGGCTAT GATTCCTGCT CATTTCCTTC GTCCTCCTAC TTCCTCACCA 
TCGCCAGATT CCCATCAAGG CCCTATCGTG ACAGTGACAA TAAAACAGCC TGATAATCTA 
CCTGGAGATG TTAATTTTCC TCCGGCTCCT ACAACACTTG GTAAAGTTAC TGAGACCATC 
ACAAAGAGCA CTCTCACCGA GACCGTCGTA ACTAGGGTGA CTGAAAATCA ATTGGTACCA 
CCAGTAATTA TTGAGGACGT GATTCTTGTA AAAGAGGGAT CTCTAGGATT TAGTATTATT 
GGTGGTACGG ATCATTCATG CACTCCATTT GGTGCAAAGG AACCTGGAAT ATTTATATCT 
CATGTCGTGC CTGGTGGTAT AGCTGCAAAA TCTGGTAAAT TAAGAATGGG TGATAGAATA 
CTAAAGGTAA ATGGTACTGA TGTAACAAAA GCTACTCATC AAGAGGCTGT GATGGAACTT 
TTGCGACCAG GAGATCAAAT AGTGCTTACT GTCCAACACG ATCCACTACC CGAAAATTAT 
CAGCTGGTCG AAATAGAGTA CATCCCTGTG ACAGAATTAG TTATTACAAA AGAACCAGGA 
GAGAAACTGG GCATGCATAT TAAAGGTGGA CTTCGAGGAC AAAAAGGTAA TCCACTTGAT 
CATACAGATG AAGGGGTTTT TATATCCAAA ATCAACTCAG GTGGTGCAGC TAAAAGAGAT 
GGAAGACTGA AAGTTGGAAT GAGACTATTA GAAGTTAATG GTACATCATT ATTAGGCGCA 
ACTCACCAAG AAGCAGTAAA TATATTGCGA TGTTCAGGAA ATACGATTAC TTTGGTGGTT 
TGTAAAGGAT ATGATAAGAG TGAAATTGAA CCAGTATTAC CAATATCCGA TGGAAGAGAT 
TCTAAGGAAT CTAGAACATC CAAAGAATTA AAGGATGCTA CAACAGAAGG TACTAAATCG 
TTATCACAAA GTATATCCAG TCTGGATCGC GATGATGAAG AAGCAGCAAC TCTTAGACAA 
GAACAGGAAA TGAAAGCTGA ACTTGTCGCA TGGGAACAAG AAGAAAGAGA ACGTGCTTTG 
CTTGAGCATA GAGAAAAATC TACTCCTGAA AAAGTATTAG ATGTAGTAAG AGCAGCTGAA 
TCATTAGTAA GCAAATCAAA TAGTCCGGTT GATATGGTCG TACCACCAAA GTCACCAGGT 
GGTACGAAAG ATCTCAAAAC AACCACTATT GTGATGAGCA AACATACTCT AGCACCTCAA 
AATACAAATC TTTCTCCAAC AACATCACCA ACACCACCTC TAGTAAAGAT GTCGGTTAGT 
GATAAAAAAA AATTGTTTGA AAGTGCCATG GAGGAACATT TAAAACCTTC CCCTAAACCA 
GAAAAAGTAT TCAGTTTTCT AAGTCAGGAT GAAGTTGAGA AAATGAAACA AGAAGAAGAA 
AAGAAGATTG CTACTCTAAC GCGGGATGAA TTAAAGTCGT GGGCACAACT TGATGAAAAT 
GAAGGTTTGG AAGATTTAGA AGAAACATTA GAAGATCAGG ATAATCGGCG ACCTAATAGC 
CGACTGAGTT CGCGAACTTC GATCCCTCTT CTGCAAAATC TTCCAAGCAG TGTAAGGACA 
GCAAAAGCAG AACGCCGCTT GAAAGAAAGA TTAATACAGG AGGGTATAAT TTCGGATGAA 
GATGAAGAAA GTCATTTAAG TCCTGCCGAA CAGAGAGCGT TAAGAGCAGA AAAACGTGCG 
GCATGGAGAC AAGCACGTTT GAAATCTCTG GAACAAGATG CTCTTCAAGC ACAGATAGTA 
ATTAAAAAAA TGAGTGAAAT GATGGACACG AATAAAACAA GTTCAATGCA GGATTCTACA 
GATATTCCTA TTACTCCTGT TTCTGAATCG GAAAAGACAA CTGATTTCAC TACGTTACGG 
CCGTCTAGTG CAGATTTTCC CAAACTTGCT GTACGTAGCA AAGTGGGTCC CCCTAAAGAG 
ATACGCGAAT CCGAGAAAGT AGTGGACGAG AAGGTCACTC GACGTACTGA GGAATATGTG 
GATGAGGTGA CGGGTGAACG ACGTGTGCGA ACAGTCGAAT ACGTCGAGAA ACTCATTGAA 
CGACAGGTTG AAACTCTACG AGAAAAGATA ATATCATTGG AATTAAGTAA TGCAGAAGAT 
GAAATAGAAA GTATTACCGG TACAGGAGCA AGTGATGCTG AAAGCGAAAG CGAAGAAATT 
ACTGTACAAC CTCCTACTAT GACTACTTTA CAAGAAAGAA ATGAAACAAT TATATCTACA 
AATGAAGGTG CCGCACCTAA AATAAGTGCA AATACTGTTC TTGTTTCAAA AAAAAAGAAA 
CGAAAACGTT CGAAGAAAGG TCGTCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108003564 ( XM_017065898.2 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_016921387.1 Apis cerana protein lap4 (LOC108003564), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OAp29731
Clone ID Related Accession (Same CDS sequence)	XM_017065898.2 , XM_017065898.1
Accession Version	XM_017065898.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-16
Organism	Apis cerana(Asiatic honeybee)
Product	protein lap4 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016017567.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 17, 2019 this sequence version replaced XM_017065898.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis cerana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGC AGATATCATC GAGAAAAAAC 
TCAGTGTGCT CTAGTTTAAG TACTAGTAGA GCTCCAAGTG CAACATCTCA TGTTTCATCT 
ATAGGTTTTT CACATAATTT AGAAAATGGT GATGCTTTAT CTCATGATGC TATTAAGTGT 
AAGAAAATTC CTGAACCTAT ATCTTCAACA AAAGTAGGCA ATGAACCAAT GGTATCAGTT 
CTTGTTCACA CAACATTAAT ACGGGACCAA AATGGGCTTG GATTTAGTAT TGCTGGTGGA 
GAAGGTTCTC CACCATTCAA AGACAATAGT GATGCGATAT ATATTTCAAG AATAACAGAT 
GGCGGCGTAG CACAAAAAGA TGGTAAATTA TTAGTTGGAG ACAAAGTAAT ATCTATTAAT 
GGAGTTGAGA TGAGAGGAGC TAAGCATGAA CAAGCAGTTG CTTTATTAAC AGGTTTAGAA 
AGATTTGTTC GATTAGTAGT TGAACGCGAA ATACCATTTT CGCAAGCAAA TGCAGCTACG 
GTCGTAACAC CTTCAGAAAA ATCACCACGA GTGATCGGTG CACCTAAACC ATATACAGGA 
TTATATAATG CCAATAGTTA TATAGCAAAT AAACCGGGAT ATACTGGATA CAGACGTTCT 
ATAGATGCTG ATAGAACATT ATCTCCAAGC CCTACTTCGA AAACGCCTCC ACCTATGAAA 
ATTGAAACTA CTGAATTACC GAAAATGAAC GGTGTTACAG AATCAGGAAA TATTAAAAAT 
GTTTCTTCGA TATCACCAAG TCCAACAAAT AGCCAACTAC ATCCACAGCC TGCTCCTAGG 
CACAGCATTT CGCAACCAAC AATTACACCT ACAACTACTA CGAGTTCAAC GCCCACATCT 
TCTACAGAAC CTAGGTCAAC CTCACCCCAG GAAGATATAC AGGTACCCAA GCCTATCACC 
AATGAAGAAT TTCAGGCTAT GATTCCTGCT CATTTCCTTC GTCCTCCTAC TTCCTCACCA 
TCGCCAGATT CCCATCAAGG CCCTATCGTG ACAGTGACAA TAAAACAGCC TGATAATCTA 
CCTGGAGATG TTAATTTTCC TCCGGCTCCT ACAACACTTG GTAAAGTTAC TGAGACCATC 
ACAAAGAGCA CTCTCACCGA GACCGTCGTA ACTAGGGTGA CTGAAAATCA ATTGGTACCA 
CCAGTAATTA TTGAGGACGT GATTCTTGTA AAAGAGGGAT CTCTAGGATT TAGTATTATT 
GGTGGTACGG ATCATTCATG CACTCCATTT GGTGCAAAGG AACCTGGAAT ATTTATATCT 
CATGTCGTGC CTGGTGGTAT AGCTGCAAAA TCTGGTAAAT TAAGAATGGG TGATAGAATA 
CTAAAGGTAA ATGGTACTGA TGTAACAAAA GCTACTCATC AAGAGGCTGT GATGGAACTT 
TTGCGACCAG GAGATCAAAT AGTGCTTACT GTCCAACACG ATCCACTACC CGAAAATTAT 
CAGCTGGTCG AAATAGAGTA CATCCCTGTG ACAGAATTAG TTATTACAAA AGAACCAGGA 
GAGAAACTGG GCATGCATAT TAAAGGTGGA CTTCGAGGAC AAAAAGGTAA TCCACTTGAT 
CATACAGATG AAGGGGTTTT TATATCCAAA ATCAACTCAG GTGGTGCAGC TAAAAGAGAT 
GGAAGACTGA AAGTTGGAAT GAGACTATTA GAAGTTAATG GTACATCATT ATTAGGCGCA 
ACTCACCAAG AAGCAGTAAA TATATTGCGA TGTTCAGGAA ATACGATTAC TTTGGTGGTT 
TGTAAAGGAT ATGATAAGAG TGAAATTGAA CCAGTATTAC CAATATCCGA TGGAAGAGAT 
TCTAAGGAAT CTAGAACATC CAAAGAATTA AAGGATGCTA CAACAGAAGG TACTAAATCG 
TTATCACAAA GTATATCCAG TCTGGATCGC GATGATGAAG AAGCAGCAAC TCTTAGACAA 
GAACAGGAAA TGAAAGCTGA ACTTGTCGCA TGGGAACAAG AAGAAAGAGA ACGTGCTTTG 
CTTGAGCATA GAGAAAAATC TACTCCTGAA AAAGTATTAG ATGTAGTAAG AGCAGCTGAA 
TCATTAGTAA GCAAATCAAA TAGTCCGGTT GATATGGTCG TACCACCAAA GTCACCAGGT 
GGTACGAAAG ATCTCAAAAC AACCACTATT GTGATGAGCA AACATACTCT AGCACCTCAA 
AATACAAATA CAGTGGAAGG TGATAACGCT ACTTTGGCTG CCCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016921387.1
CDS	1476..6143
Translation	MFRCIPIFKGCNRQVEYIDKRHCSLPCVPDDILRYSRSLEELLLDANHIRDLPKNFFRLQKLRKLGLSDNEIHRLPPDIQNFENLVELDVSRNDIPDIPENIKNLRALQVADFSSNPIPRLPAGFVQLRNLTVLGLNDMSLTNLPPDFGSLEALQSLELRENLLKSLPESLSQLYKLERLDLGDNDIEVLPAHIGELPALQELWLDHNQLQHLPPEIGELKTLVCLDVSENRLEDLPEEIGGLEALTDLHLSQNVIEKLPDGLGELKKLTILKVDQNRLSTLNPNIGRCENLQELILTENFLLELPVSIGKLLNLNNLNVDRNSLQSLPTETGNLKQLGVLSLRDNKLQYLPIEVGQCTALHVLDVSGNRLQYLPYSLINLNLKAVWLSKNQAQPMLTFQTDVDEETGQEVLTCFLLPQLEIHPDDSGRLGMLAGMRNAVQDGEIRELGSSDDEGWQEKEASRTHSVKFTDEPPETDKETPFVRQNTPHPKELKAKAHKLFSKGKNDSRSASTDEQDVSQTFGLDKTETDVSTKPNDLTVDLIEQESLKHESVENEQKEIVPGVTDNIDVQSSNEPEIVSNQYIVESNTDVRTEGSISELKDRNDKEDDESENEETQRHVEFSIAEGTDYDGSGDSNRPNRLHRRDTPHHLKNKRIHTAVDKEKVASIIAQALMKKNDEISTSTSAPAETTENFDAQSQDAISDAEPTIEVREEQYEIHIERTTGGLGLSIAGGIGSTPFKGDDEGIFISRVTEGGPADLAGLKVEDKVLSVNGVSVVNVGHYDAVEVLKACGRVLVLVVQREVTRIVPPFEQQISSRKNSVCSSLSTSRAPSATSHVSSIGFSHNLENGDALSHDAIKCKKIPEPISSTKVGNEPMVSVLVHTTLIRDQNGLGFSIAGGEGSPPFKDNSDAIYISRITDGGVAQKDGKLLVGDKVISINGVEMRGAKHEQAVALLTGLERFVRLVVEREIPFSQANAATVVTPSEKSPRVIGAPKPYTGLYNANSYIANKPGYTGYRRSIDADRTLSPSPTSKTPPPMKIETTELPKMNGVTESGNIKNVSSISPSPTNSQLHPQPAPRHSISQPTITPTTTTSSTPTSSTEPRSTSPQEDIQVPKPITNEEFQAMIPAHFLRPPTSSPSPDSHQGPIVTVTIKQPDNLPGDVNFPPAPTTLGKVTETITKSTLTETVVTRVTENQLVPPVIIEDVILVKEGSLGFSIIGGTDHSCTPFGAKEPGIFISHVVPGGIAAKSGKLRMGDRILKVNGTDVTKATHQEAVMELLRPGDQIVLTVQHDPLPENYQLVEIEYIPVTELVITKEPGEKLGMHIKGGLRGQKGNPLDHTDEGVFISKINSGGAAKRDGRLKVGMRLLEVNGTSLLGATHQEAVNILRCSGNTITLVVCKGYDKSEIEPVLPISDGRDSKESRTSKELKDATTEGTKSLSQSISSLDRDDEEAATLRQEQEMKAELVAWEQEERERALLEHREKSTPEKVLDVVRAAESLVSKSNSPVDMVVPPKSPGGTKDLKTTTIVMSKHTLAPQNTNTVEGDNATLAAL

Target ORF information:

RefSeq Version	XM_017065898.2
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana protein lap4 (LOC108003564), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017065898.2

ORF Insert Sequence:

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGC AGATATCATC GAGAAAAAAC 
TCAGTGTGCT CTAGTTTAAG TACTAGTAGA GCTCCAAGTG CAACATCTCA TGTTTCATCT 
ATAGGTTTTT CACATAATTT AGAAAATGGT GATGCTTTAT CTCATGATGC TATTAAGTGT 
AAGAAAATTC CTGAACCTAT ATCTTCAACA AAAGTAGGCA ATGAACCAAT GGTATCAGTT 
CTTGTTCACA CAACATTAAT ACGGGACCAA AATGGGCTTG GATTTAGTAT TGCTGGTGGA 
GAAGGTTCTC CACCATTCAA AGACAATAGT GATGCGATAT ATATTTCAAG AATAACAGAT 
GGCGGCGTAG CACAAAAAGA TGGTAAATTA TTAGTTGGAG ACAAAGTAAT ATCTATTAAT 
GGAGTTGAGA TGAGAGGAGC TAAGCATGAA CAAGCAGTTG CTTTATTAAC AGGTTTAGAA 
AGATTTGTTC GATTAGTAGT TGAACGCGAA ATACCATTTT CGCAAGCAAA TGCAGCTACG 
GTCGTAACAC CTTCAGAAAA ATCACCACGA GTGATCGGTG CACCTAAACC ATATACAGGA 
TTATATAATG CCAATAGTTA TATAGCAAAT AAACCGGGAT ATACTGGATA CAGACGTTCT 
ATAGATGCTG ATAGAACATT ATCTCCAAGC CCTACTTCGA AAACGCCTCC ACCTATGAAA 
ATTGAAACTA CTGAATTACC GAAAATGAAC GGTGTTACAG AATCAGGAAA TATTAAAAAT 
GTTTCTTCGA TATCACCAAG TCCAACAAAT AGCCAACTAC ATCCACAGCC TGCTCCTAGG 
CACAGCATTT CGCAACCAAC AATTACACCT ACAACTACTA CGAGTTCAAC GCCCACATCT 
TCTACAGAAC CTAGGTCAAC CTCACCCCAG GAAGATATAC AGGTACCCAA GCCTATCACC 
AATGAAGAAT TTCAGGCTAT GATTCCTGCT CATTTCCTTC GTCCTCCTAC TTCCTCACCA 
TCGCCAGATT CCCATCAAGG CCCTATCGTG ACAGTGACAA TAAAACAGCC TGATAATCTA 
CCTGGAGATG TTAATTTTCC TCCGGCTCCT ACAACACTTG GTAAAGTTAC TGAGACCATC 
ACAAAGAGCA CTCTCACCGA GACCGTCGTA ACTAGGGTGA CTGAAAATCA ATTGGTACCA 
CCAGTAATTA TTGAGGACGT GATTCTTGTA AAAGAGGGAT CTCTAGGATT TAGTATTATT 
GGTGGTACGG ATCATTCATG CACTCCATTT GGTGCAAAGG AACCTGGAAT ATTTATATCT 
CATGTCGTGC CTGGTGGTAT AGCTGCAAAA TCTGGTAAAT TAAGAATGGG TGATAGAATA 
CTAAAGGTAA ATGGTACTGA TGTAACAAAA GCTACTCATC AAGAGGCTGT GATGGAACTT 
TTGCGACCAG GAGATCAAAT AGTGCTTACT GTCCAACACG ATCCACTACC CGAAAATTAT 
CAGCTGGTCG AAATAGAGTA CATCCCTGTG ACAGAATTAG TTATTACAAA AGAACCAGGA 
GAGAAACTGG GCATGCATAT TAAAGGTGGA CTTCGAGGAC AAAAAGGTAA TCCACTTGAT 
CATACAGATG AAGGGGTTTT TATATCCAAA ATCAACTCAG GTGGTGCAGC TAAAAGAGAT 
GGAAGACTGA AAGTTGGAAT GAGACTATTA GAAGTTAATG GTACATCATT ATTAGGCGCA 
ACTCACCAAG AAGCAGTAAA TATATTGCGA TGTTCAGGAA ATACGATTAC TTTGGTGGTT 
TGTAAAGGAT ATGATAAGAG TGAAATTGAA CCAGTATTAC CAATATCCGA TGGAAGAGAT 
TCTAAGGAAT CTAGAACATC CAAAGAATTA AAGGATGCTA CAACAGAAGG TACTAAATCG 
TTATCACAAA GTATATCCAG TCTGGATCGC GATGATGAAG AAGCAGCAAC TCTTAGACAA 
GAACAGGAAA TGAAAGCTGA ACTTGTCGCA TGGGAACAAG AAGAAAGAGA ACGTGCTTTG 
CTTGAGCATA GAGAAAAATC TACTCCTGAA AAAGTATTAG ATGTAGTAAG AGCAGCTGAA 
TCATTAGTAA GCAAATCAAA TAGTCCGGTT GATATGGTCG TACCACCAAA GTCACCAGGT 
GGTACGAAAG ATCTCAAAAC AACCACTATT GTGATGAGCA AACATACTCT AGCACCTCAA 
AATACAAATA CAGTGGAAGG TGATAACGCT ACTTTGGCTG CCCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108003564 ( XM_017065898.1 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_016921387.1 Apis cerana protein lap4 (LOC108003564), transcript variant X13, mRNA.

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CloneID	OAp29731
Clone ID Related Accession (Same CDS sequence)	XM_017065898.2 , XM_017065898.1
Accession Version	XM_017065898.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-08
Organism	Apis cerana(Asiatic honeybee)
Product	protein lap4 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016017567.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis cerana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGC AGATATCATC GAGAAAAAAC 
TCAGTGTGCT CTAGTTTAAG TACTAGTAGA GCTCCAAGTG CAACATCTCA TGTTTCATCT 
ATAGGTTTTT CACATAATTT AGAAAATGGT GATGCTTTAT CTCATGATGC TATTAAGTGT 
AAGAAAATTC CTGAACCTAT ATCTTCAACA AAAGTAGGCA ATGAACCAAT GGTATCAGTT 
CTTGTTCACA CAACATTAAT ACGGGACCAA AATGGGCTTG GATTTAGTAT TGCTGGTGGA 
GAAGGTTCTC CACCATTCAA AGACAATAGT GATGCGATAT ATATTTCAAG AATAACAGAT 
GGCGGCGTAG CACAAAAAGA TGGTAAATTA TTAGTTGGAG ACAAAGTAAT ATCTATTAAT 
GGAGTTGAGA TGAGAGGAGC TAAGCATGAA CAAGCAGTTG CTTTATTAAC AGGTTTAGAA 
AGATTTGTTC GATTAGTAGT TGAACGCGAA ATACCATTTT CGCAAGCAAA TGCAGCTACG 
GTCGTAACAC CTTCAGAAAA ATCACCACGA GTGATCGGTG CACCTAAACC ATATACAGGA 
TTATATAATG CCAATAGTTA TATAGCAAAT AAACCGGGAT ATACTGGATA CAGACGTTCT 
ATAGATGCTG ATAGAACATT ATCTCCAAGC CCTACTTCGA AAACGCCTCC ACCTATGAAA 
ATTGAAACTA CTGAATTACC GAAAATGAAC GGTGTTACAG AATCAGGAAA TATTAAAAAT 
GTTTCTTCGA TATCACCAAG TCCAACAAAT AGCCAACTAC ATCCACAGCC TGCTCCTAGG 
CACAGCATTT CGCAACCAAC AATTACACCT ACAACTACTA CGAGTTCAAC GCCCACATCT 
TCTACAGAAC CTAGGTCAAC CTCACCCCAG GAAGATATAC AGGTACCCAA GCCTATCACC 
AATGAAGAAT TTCAGGCTAT GATTCCTGCT CATTTCCTTC GTCCTCCTAC TTCCTCACCA 
TCGCCAGATT CCCATCAAGG CCCTATCGTG ACAGTGACAA TAAAACAGCC TGATAATCTA 
CCTGGAGATG TTAATTTTCC TCCGGCTCCT ACAACACTTG GTAAAGTTAC TGAGACCATC 
ACAAAGAGCA CTCTCACCGA GACCGTCGTA ACTAGGGTGA CTGAAAATCA ATTGGTACCA 
CCAGTAATTA TTGAGGACGT GATTCTTGTA AAAGAGGGAT CTCTAGGATT TAGTATTATT 
GGTGGTACGG ATCATTCATG CACTCCATTT GGTGCAAAGG AACCTGGAAT ATTTATATCT 
CATGTCGTGC CTGGTGGTAT AGCTGCAAAA TCTGGTAAAT TAAGAATGGG TGATAGAATA 
CTAAAGGTAA ATGGTACTGA TGTAACAAAA GCTACTCATC AAGAGGCTGT GATGGAACTT 
TTGCGACCAG GAGATCAAAT AGTGCTTACT GTCCAACACG ATCCACTACC CGAAAATTAT 
CAGCTGGTCG AAATAGAGTA CATCCCTGTG ACAGAATTAG TTATTACAAA AGAACCAGGA 
GAGAAACTGG GCATGCATAT TAAAGGTGGA CTTCGAGGAC AAAAAGGTAA TCCACTTGAT 
CATACAGATG AAGGGGTTTT TATATCCAAA ATCAACTCAG GTGGTGCAGC TAAAAGAGAT 
GGAAGACTGA AAGTTGGAAT GAGACTATTA GAAGTTAATG GTACATCATT ATTAGGCGCA 
ACTCACCAAG AAGCAGTAAA TATATTGCGA TGTTCAGGAA ATACGATTAC TTTGGTGGTT 
TGTAAAGGAT ATGATAAGAG TGAAATTGAA CCAGTATTAC CAATATCCGA TGGAAGAGAT 
TCTAAGGAAT CTAGAACATC CAAAGAATTA AAGGATGCTA CAACAGAAGG TACTAAATCG 
TTATCACAAA GTATATCCAG TCTGGATCGC GATGATGAAG AAGCAGCAAC TCTTAGACAA 
GAACAGGAAA TGAAAGCTGA ACTTGTCGCA TGGGAACAAG AAGAAAGAGA ACGTGCTTTG 
CTTGAGCATA GAGAAAAATC TACTCCTGAA AAAGTATTAG ATGTAGTAAG AGCAGCTGAA 
TCATTAGTAA GCAAATCAAA TAGTCCGGTT GATATGGTCG TACCACCAAA GTCACCAGGT 
GGTACGAAAG ATCTCAAAAC AACCACTATT GTGATGAGCA AACATACTCT AGCACCTCAA 
AATACAAATA CAGTGGAAGG TGATAACGCT ACTTTGGCTG CCCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016921387.1
CDS	233..4900
Translation	MFRCIPIFKGCNRQVEYIDKRHCSLPCVPDDILRYSRSLEELLLDANHIRDLPKNFFRLQKLRKLGLSDNEIHRLPPDIQNFENLVELDVSRNDIPDIPENIKNLRALQVADFSSNPIPRLPAGFVQLRNLTVLGLNDMSLTNLPPDFGSLEALQSLELRENLLKSLPESLSQLYKLERLDLGDNDIEVLPAHIGELPALQELWLDHNQLQHLPPEIGELKTLVCLDVSENRLEDLPEEIGGLEALTDLHLSQNVIEKLPDGLGELKKLTILKVDQNRLSTLNPNIGRCENLQELILTENFLLELPVSIGKLLNLNNLNVDRNSLQSLPTETGNLKQLGVLSLRDNKLQYLPIEVGQCTALHVLDVSGNRLQYLPYSLINLNLKAVWLSKNQAQPMLTFQTDVDEETGQEVLTCFLLPQLEIHPDDSGRLGMLAGMRNAVQDGEIRELGSSDDEGWQEKEASRTHSVKFTDEPPETDKETPFVRQNTPHPKELKAKAHKLFSKGKNDSRSASTDEQDVSQTFGLDKTETDVSTKPNDLTVDLIEQESLKHESVENEQKEIVPGVTDNIDVQSSNEPEIVSNQYIVESNTDVRTEGSISELKDRNDKEDDESENEETQRHVEFSIAEGTDYDGSGDSNRPNRLHRRDTPHHLKNKRIHTAVDKEKVASIIAQALMKKNDEISTSTSAPAETTENFDAQSQDAISDAEPTIEVREEQYEIHIERTTGGLGLSIAGGIGSTPFKGDDEGIFISRVTEGGPADLAGLKVEDKVLSVNGVSVVNVGHYDAVEVLKACGRVLVLVVQREVTRIVPPFEQQISSRKNSVCSSLSTSRAPSATSHVSSIGFSHNLENGDALSHDAIKCKKIPEPISSTKVGNEPMVSVLVHTTLIRDQNGLGFSIAGGEGSPPFKDNSDAIYISRITDGGVAQKDGKLLVGDKVISINGVEMRGAKHEQAVALLTGLERFVRLVVEREIPFSQANAATVVTPSEKSPRVIGAPKPYTGLYNANSYIANKPGYTGYRRSIDADRTLSPSPTSKTPPPMKIETTELPKMNGVTESGNIKNVSSISPSPTNSQLHPQPAPRHSISQPTITPTTTTSSTPTSSTEPRSTSPQEDIQVPKPITNEEFQAMIPAHFLRPPTSSPSPDSHQGPIVTVTIKQPDNLPGDVNFPPAPTTLGKVTETITKSTLTETVVTRVTENQLVPPVIIEDVILVKEGSLGFSIIGGTDHSCTPFGAKEPGIFISHVVPGGIAAKSGKLRMGDRILKVNGTDVTKATHQEAVMELLRPGDQIVLTVQHDPLPENYQLVEIEYIPVTELVITKEPGEKLGMHIKGGLRGQKGNPLDHTDEGVFISKINSGGAAKRDGRLKVGMRLLEVNGTSLLGATHQEAVNILRCSGNTITLVVCKGYDKSEIEPVLPISDGRDSKESRTSKELKDATTEGTKSLSQSISSLDRDDEEAATLRQEQEMKAELVAWEQEERERALLEHREKSTPEKVLDVVRAAESLVSKSNSPVDMVVPPKSPGGTKDLKTTTIVMSKHTLAPQNTNTVEGDNATLAAL

Target ORF information:

RefSeq Version	XM_017065898.1
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana protein lap4 (LOC108003564), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017065898.1

ORF Insert Sequence:

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGC AGATATCATC GAGAAAAAAC 
TCAGTGTGCT CTAGTTTAAG TACTAGTAGA GCTCCAAGTG CAACATCTCA TGTTTCATCT 
ATAGGTTTTT CACATAATTT AGAAAATGGT GATGCTTTAT CTCATGATGC TATTAAGTGT 
AAGAAAATTC CTGAACCTAT ATCTTCAACA AAAGTAGGCA ATGAACCAAT GGTATCAGTT 
CTTGTTCACA CAACATTAAT ACGGGACCAA AATGGGCTTG GATTTAGTAT TGCTGGTGGA 
GAAGGTTCTC CACCATTCAA AGACAATAGT GATGCGATAT ATATTTCAAG AATAACAGAT 
GGCGGCGTAG CACAAAAAGA TGGTAAATTA TTAGTTGGAG ACAAAGTAAT ATCTATTAAT 
GGAGTTGAGA TGAGAGGAGC TAAGCATGAA CAAGCAGTTG CTTTATTAAC AGGTTTAGAA 
AGATTTGTTC GATTAGTAGT TGAACGCGAA ATACCATTTT CGCAAGCAAA TGCAGCTACG 
GTCGTAACAC CTTCAGAAAA ATCACCACGA GTGATCGGTG CACCTAAACC ATATACAGGA 
TTATATAATG CCAATAGTTA TATAGCAAAT AAACCGGGAT ATACTGGATA CAGACGTTCT 
ATAGATGCTG ATAGAACATT ATCTCCAAGC CCTACTTCGA AAACGCCTCC ACCTATGAAA 
ATTGAAACTA CTGAATTACC GAAAATGAAC GGTGTTACAG AATCAGGAAA TATTAAAAAT 
GTTTCTTCGA TATCACCAAG TCCAACAAAT AGCCAACTAC ATCCACAGCC TGCTCCTAGG 
CACAGCATTT CGCAACCAAC AATTACACCT ACAACTACTA CGAGTTCAAC GCCCACATCT 
TCTACAGAAC CTAGGTCAAC CTCACCCCAG GAAGATATAC AGGTACCCAA GCCTATCACC 
AATGAAGAAT TTCAGGCTAT GATTCCTGCT CATTTCCTTC GTCCTCCTAC TTCCTCACCA 
TCGCCAGATT CCCATCAAGG CCCTATCGTG ACAGTGACAA TAAAACAGCC TGATAATCTA 
CCTGGAGATG TTAATTTTCC TCCGGCTCCT ACAACACTTG GTAAAGTTAC TGAGACCATC 
ACAAAGAGCA CTCTCACCGA GACCGTCGTA ACTAGGGTGA CTGAAAATCA ATTGGTACCA 
CCAGTAATTA TTGAGGACGT GATTCTTGTA AAAGAGGGAT CTCTAGGATT TAGTATTATT 
GGTGGTACGG ATCATTCATG CACTCCATTT GGTGCAAAGG AACCTGGAAT ATTTATATCT 
CATGTCGTGC CTGGTGGTAT AGCTGCAAAA TCTGGTAAAT TAAGAATGGG TGATAGAATA 
CTAAAGGTAA ATGGTACTGA TGTAACAAAA GCTACTCATC AAGAGGCTGT GATGGAACTT 
TTGCGACCAG GAGATCAAAT AGTGCTTACT GTCCAACACG ATCCACTACC CGAAAATTAT 
CAGCTGGTCG AAATAGAGTA CATCCCTGTG ACAGAATTAG TTATTACAAA AGAACCAGGA 
GAGAAACTGG GCATGCATAT TAAAGGTGGA CTTCGAGGAC AAAAAGGTAA TCCACTTGAT 
CATACAGATG AAGGGGTTTT TATATCCAAA ATCAACTCAG GTGGTGCAGC TAAAAGAGAT 
GGAAGACTGA AAGTTGGAAT GAGACTATTA GAAGTTAATG GTACATCATT ATTAGGCGCA 
ACTCACCAAG AAGCAGTAAA TATATTGCGA TGTTCAGGAA ATACGATTAC TTTGGTGGTT 
TGTAAAGGAT ATGATAAGAG TGAAATTGAA CCAGTATTAC CAATATCCGA TGGAAGAGAT 
TCTAAGGAAT CTAGAACATC CAAAGAATTA AAGGATGCTA CAACAGAAGG TACTAAATCG 
TTATCACAAA GTATATCCAG TCTGGATCGC GATGATGAAG AAGCAGCAAC TCTTAGACAA 
GAACAGGAAA TGAAAGCTGA ACTTGTCGCA TGGGAACAAG AAGAAAGAGA ACGTGCTTTG 
CTTGAGCATA GAGAAAAATC TACTCCTGAA AAAGTATTAG ATGTAGTAAG AGCAGCTGAA 
TCATTAGTAA GCAAATCAAA TAGTCCGGTT GATATGGTCG TACCACCAAA GTCACCAGGT 
GGTACGAAAG ATCTCAAAAC AACCACTATT GTGATGAGCA AACATACTCT AGCACCTCAA 
AATACAAATA CAGTGGAAGG TGATAACGCT ACTTTGGCTG CCCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108003564 ( XM_017065887.1 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_016921376.1 Apis cerana protein lap4 (LOC108003564), transcript variant X1, mRNA.

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CloneID	OAp29721
Clone ID Related Accession (Same CDS sequence)	XM_017065887.1 , XM_017065887.2
Accession Version	XM_017065887.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-08
Organism	Apis cerana(Asiatic honeybee)
Product	protein lap4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016017567.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis cerana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGC AGATATCATC GAGAAAAAAC 
TCAGTGTGCT CTAGTTTAAG TACTAGTAGA GCTCCAAGTG CAACATCTCA TGTTTCATCT 
ATAGGTTTTT CACATAATTT AGAAAATGGT GATGCTTTAT CTCATGATGC TATTAAGTGT 
AAGAAAATTC CTGAACCTAT ATCTTCAACA AAAGTAGGCA ATGAACCAAT GGTATCAGTT 
CTTGTTCACA CAACATTAAT ACGGGACCAA AATGGGCTTG GATTTAGTAT TGCTGGTGGA 
GAAGGTTCTC CACCATTCAA AGACAATAGT GATGCGATAT ATATTTCAAG AATAACAGAT 
GGCGGCGTAG CACAAAAAGA TGGTAAATTA TTAGTTGGAG ACAAAGTAAT ATCTATTAAT 
GGAGTTGAGA TGAGAGGAGC TAAGCATGAA CAAGCAGTTG CTTTATTAAC AGGTTTAGAA 
AGATTTGTTC GATTAGTAGT TGAACGCGAA ATACCATTTT CGCAAGCAAA TGCAGCTACG 
GTCGTAACAC CTTCAGAAAA ATCACCACGA GTGATCGGTG CACCTAAACC ATATACAGGA 
TTATATAATG CCAATAGTTA TATAGCAAAT AAACCGGGAT ATACTGGATA CAGACGTTCT 
ATAGATGCTG ATAGAACATT ATCTCCAAGC CCTACTTCGA AAACGCCTCC ACCTATGAAA 
ATTGAAACTA CTGAATTACC GAAAATGAAC GGTGTTACAG AATCAGGAAA TATTAAAAAT 
GTTTCTTCGA TATCACCAAG TCCAACAAAT AGCCAACTAC ATCCACAGCC TGCTCCTAGG 
CACAGCATTT CGCAACCAAC AATTACACCT ACAACTACTA CGAGTTCAAC GCCCACATCT 
TCTACAGAAC CTAGGTCAAC CTCACCCCAG GAAGATATAC AGGTACCCAA GCCTATCACC 
AATGAAGAAT TTCAGGCTAT GATTCCTGCT CATTTCCTTC GTCCTCCTAC TTCCTCACCA 
TCGCCAGATT CCCATCAAGG CCCTATCGTG ACAGTGACAA TAAAACAGCC TGATAATCTA 
CCTGGAGATG TTAATTTTCC TCCGGCTCCT ACAACACTTG GTAAAGTTAC TGAGACCATC 
ACAAAGAGCA CTCTCACCGA GACCGTCGTA ACTAGGGTGA CTGAAAATCA ATTGGTACCA 
CCAGTAATTA TTGAGGACGT GATTCTTGTA AAAGAGGGAT CTCTAGGATT TAGTATTATT 
GGTGGTACGG ATCATTCATG CACTCCATTT GGTGCAAAGG AACCTGGAAT ATTTATATCT 
CATGTCGTGC CTGGTGGTAT AGCTGCAAAA TCTGGTAAAT TAAGAATGGG TGATAGAATA 
CTAAAGGTAA ATGGTACTGA TGTAACAAAA GCTACTCATC AAGAGGCTGT GATGGAACTT 
TTGCGACCAG GAGATCAAAT AGTGCTTACT GTCCAACACG ATCCACTACC CGAAAATTAT 
CAGCTGGTCG AAATAGAGTA CATCCCTGTG ACAGAATTAG TTATTACAAA AGAACCAGGA 
GAGAAACTGG GCATGCATAT TAAAGGTGGA CTTCGAGGAC AAAAAGGTAA TCCACTTGAT 
CATACAGATG AAGGGGTTTT TATATCCAAA ATCAACTCAG GTGGTGCAGC TAAAAGAGAT 
GGAAGACTGA AAGTTGGAAT GAGACTATTA GAAGTTAATG GTACATCATT ATTAGGCGCA 
ACTCACCAAG AAGCAGTAAA TATATTGCGA TGTTCAGGAA ATACGATTAC TTTGGTGGTT 
TGTAAAGGAT ATGATAAGAG TGAAATTGAA CCAGTATTAC CAATATCCGA TGGAAGAGAT 
TCTAAGGAAT CTAGAACATC CAAAGAATTA AAGGATGCTA CAACAGAAGG TACTAAATCG 
TTATCACAAA GTATATCCAG TCTGGATCGC GATGATGAAG AAGCAGCAAC TCTTAGACAA 
GAACAGGAAA TGAAAGCTGA ACTTGTCGCA TGGGAACAAG AAGAAAGAGA ACGTGCTTTG 
CTTGAGCATA GAGAAAAATC TACTCCTGAA AAAGTATTAG ATGTAGTAAG AGCAGCTGAA 
TCATTAGTAA GCAAATCAAA TAGTCCGGTT GATATGGTCG TACCACCAAA GTCACCAGGT 
GGTACGAAAG ATCTCAAAAC AACCACTATT GTGATGAGCA AACATACTCT AGCACCTCAA 
AATACAAATT CACTGAGGAA AGAGAGAACT GGAACATCAG TGGACAATCC ATCGCCCAAG 
TCTCCGATCT CTACTTTAAC TCCATCGATG AATTTCGATC ACACTACTGC TGTCCAAAGC 
TTGCCCTCTC CAAAGAAAAA AAGTTCCATA CCAAAACGTA GTATTACATT TGCTGATCTT 
CCTCCCATTT CTAAAGAACC AATTAATTAT TCCATTTCGG CGAAAGAAGA TCCATCACCT 
TTGCATGATA GTAAATTTAC TTTCGATCCT CAAACTATTT CTTATAAGAA AGTCTATCAA 
AATCCTATCC AAGCTACTCA TTCTCGTTTC AAACTTCCTC CTACACCTTT AACTGCTCCT 
AAATCTATAG AAGATTATGG TACTTCAATT TCTTTTGGTA AACGACAGAA CGCACGCTCT 
GTTGATGGGA ATAGTCAGGT TGAGCTTTCT CCAACAACAT CACCAACACC ACCTCTAGTA 
AAGATGTCGG TTAGTGATAA AAAAAAATTG TTTGAAAGTG CCATGGAGGA ACATTTAAAA 
CCTTCCCCTA AACCAGAAAA AGTATTCAGT TTTCTAAGTC AGGATGAAGT TGAGAAAATG 
AAACAAGAAG AAGAAAAGAA GATTGCTACT CTAACGCGGG ATGAATTAAA GTCGTGGGCA 
CAACTTGATG AAAATGAAGG TTTGGAAGAT TTAGAAGAAA CATTAGAAGA TCAGGATAAT 
CGGCGACCTA ATAGCCGACT GAGTTCGCGA ACTTCGATCC CTCTTCTGCA AAATCTTCCA 
AGCAGTGTAA GGACAGCAAA AGCAGAACGC CGCTTGAAAG AAAGATTAAT ACAGGAGGGT 
ATAATTTCGG ATGAAGATGA AGAAAGTCAT TTAAGTCCTG CCGAACAGAG AGCGTTAAGA 
GCAGAAAAAC GTGCGGCATG GAGACAAGCA CGTTTGAAAT CTCTGGAACA AGATGCTCTT 
CAAGCACAGA TAGTAATTAA AAAAATGAGT GAAATGATGG ACACGAATAA AACAAGTTCA 
ATGCAGGATT CTACAGATAT TCCTATTACT CCTGTTTCTG AATCGGAAAA GACAACTGAT 
TTCACTACGT TACGGCCGTC TAGTGCAGAT TTTCCCAAAC TTGCTGTACG TAGCAAAGTG 
GGTCCCCCTA AAGAGATACG CGAATCCGAG AAAGTAGTGG ACGAGAAGGT CACTCGACGT 
ACTGAGGAAT ATGTGGATGA GGTGACGGGT GAACGACGTG TGCGAACAGT CGAATACGTC 
GAGAAACTCA TTGAACGACA GGTTGAAACT CTACGAGAAA AGATAATATC ATTGGAATTA 
AGTAATGCAG AAGATGAAAT AGAAAGTATT ACCGGTACAG GAGCAAGTGA TGCTGAAAGC 
GAAAGCGAAG AAATTACTGT ACAACCTCCT ACTATGACTA CTTTACAAGA AAGAAATGAA 
ACAATTATAT CTACAAATGA AGGTGCCGCA CCTAAAATAA GTGCAAATAC TGTTCTTGTT 
TCAAAAAAAA AGAAACGAAA ACGTTCGAAG AAAGGTCGTC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016921376.1
CDS	233..6397
Translation	MFRCIPIFKGCNRQVEYIDKRHCSLPCVPDDILRYSRSLEELLLDANHIRDLPKNFFRLQKLRKLGLSDNEIHRLPPDIQNFENLVELDVSRNDIPDIPENIKNLRALQVADFSSNPIPRLPAGFVQLRNLTVLGLNDMSLTNLPPDFGSLEALQSLELRENLLKSLPESLSQLYKLERLDLGDNDIEVLPAHIGELPALQELWLDHNQLQHLPPEIGELKTLVCLDVSENRLEDLPEEIGGLEALTDLHLSQNVIEKLPDGLGELKKLTILKVDQNRLSTLNPNIGRCENLQELILTENFLLELPVSIGKLLNLNNLNVDRNSLQSLPTETGNLKQLGVLSLRDNKLQYLPIEVGQCTALHVLDVSGNRLQYLPYSLINLNLKAVWLSKNQAQPMLTFQTDVDEETGQEVLTCFLLPQLEIHPDDSGRLGMLAGMRNAVQDGEIRELGSSDDEGWQEKEASRTHSVKFTDEPPETDKETPFVRQNTPHPKELKAKAHKLFSKGKNDSRSASTDEQDVSQTFGLDKTETDVSTKPNDLTVDLIEQESLKHESVENEQKEIVPGVTDNIDVQSSNEPEIVSNQYIVESNTDVRTEGSISELKDRNDKEDDESENEETQRHVEFSIAEGTDYDGSGDSNRPNRLHRRDTPHHLKNKRIHTAVDKEKVASIIAQALMKKNDEISTSTSAPAETTENFDAQSQDAISDAEPTIEVREEQYEIHIERTTGGLGLSIAGGIGSTPFKGDDEGIFISRVTEGGPADLAGLKVEDKVLSVNGVSVVNVGHYDAVEVLKACGRVLVLVVQREVTRIVPPFEQQISSRKNSVCSSLSTSRAPSATSHVSSIGFSHNLENGDALSHDAIKCKKIPEPISSTKVGNEPMVSVLVHTTLIRDQNGLGFSIAGGEGSPPFKDNSDAIYISRITDGGVAQKDGKLLVGDKVISINGVEMRGAKHEQAVALLTGLERFVRLVVEREIPFSQANAATVVTPSEKSPRVIGAPKPYTGLYNANSYIANKPGYTGYRRSIDADRTLSPSPTSKTPPPMKIETTELPKMNGVTESGNIKNVSSISPSPTNSQLHPQPAPRHSISQPTITPTTTTSSTPTSSTEPRSTSPQEDIQVPKPITNEEFQAMIPAHFLRPPTSSPSPDSHQGPIVTVTIKQPDNLPGDVNFPPAPTTLGKVTETITKSTLTETVVTRVTENQLVPPVIIEDVILVKEGSLGFSIIGGTDHSCTPFGAKEPGIFISHVVPGGIAAKSGKLRMGDRILKVNGTDVTKATHQEAVMELLRPGDQIVLTVQHDPLPENYQLVEIEYIPVTELVITKEPGEKLGMHIKGGLRGQKGNPLDHTDEGVFISKINSGGAAKRDGRLKVGMRLLEVNGTSLLGATHQEAVNILRCSGNTITLVVCKGYDKSEIEPVLPISDGRDSKESRTSKELKDATTEGTKSLSQSISSLDRDDEEAATLRQEQEMKAELVAWEQEERERALLEHREKSTPEKVLDVVRAAESLVSKSNSPVDMVVPPKSPGGTKDLKTTTIVMSKHTLAPQNTNSLRKERTGTSVDNPSPKSPISTLTPSMNFDHTTAVQSLPSPKKKSSIPKRSITFADLPPISKEPINYSISAKEDPSPLHDSKFTFDPQTISYKKVYQNPIQATHSRFKLPPTPLTAPKSIEDYGTSISFGKRQNARSVDGNSQVELSPTTSPTPPLVKMSVSDKKKLFESAMEEHLKPSPKPEKVFSFLSQDEVEKMKQEEEKKIATLTRDELKSWAQLDENEGLEDLEETLEDQDNRRPNSRLSSRTSIPLLQNLPSSVRTAKAERRLKERLIQEGIISDEDEESHLSPAEQRALRAEKRAAWRQARLKSLEQDALQAQIVIKKMSEMMDTNKTSSMQDSTDIPITPVSESEKTTDFTTLRPSSADFPKLAVRSKVGPPKEIRESEKVVDEKVTRRTEEYVDEVTGERRVRTVEYVEKLIERQVETLREKIISLELSNAEDEIESITGTGASDAESESEEITVQPPTMTTLQERNETIISTNEGAAPKISANTVLVSKKKKRKRSKKGRH

Target ORF information:

RefSeq Version	XM_017065887.1
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana protein lap4 (LOC108003564), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017065887.1

ORF Insert Sequence:

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGC AGATATCATC GAGAAAAAAC 
TCAGTGTGCT CTAGTTTAAG TACTAGTAGA GCTCCAAGTG CAACATCTCA TGTTTCATCT 
ATAGGTTTTT CACATAATTT AGAAAATGGT GATGCTTTAT CTCATGATGC TATTAAGTGT 
AAGAAAATTC CTGAACCTAT ATCTTCAACA AAAGTAGGCA ATGAACCAAT GGTATCAGTT 
CTTGTTCACA CAACATTAAT ACGGGACCAA AATGGGCTTG GATTTAGTAT TGCTGGTGGA 
GAAGGTTCTC CACCATTCAA AGACAATAGT GATGCGATAT ATATTTCAAG AATAACAGAT 
GGCGGCGTAG CACAAAAAGA TGGTAAATTA TTAGTTGGAG ACAAAGTAAT ATCTATTAAT 
GGAGTTGAGA TGAGAGGAGC TAAGCATGAA CAAGCAGTTG CTTTATTAAC AGGTTTAGAA 
AGATTTGTTC GATTAGTAGT TGAACGCGAA ATACCATTTT CGCAAGCAAA TGCAGCTACG 
GTCGTAACAC CTTCAGAAAA ATCACCACGA GTGATCGGTG CACCTAAACC ATATACAGGA 
TTATATAATG CCAATAGTTA TATAGCAAAT AAACCGGGAT ATACTGGATA CAGACGTTCT 
ATAGATGCTG ATAGAACATT ATCTCCAAGC CCTACTTCGA AAACGCCTCC ACCTATGAAA 
ATTGAAACTA CTGAATTACC GAAAATGAAC GGTGTTACAG AATCAGGAAA TATTAAAAAT 
GTTTCTTCGA TATCACCAAG TCCAACAAAT AGCCAACTAC ATCCACAGCC TGCTCCTAGG 
CACAGCATTT CGCAACCAAC AATTACACCT ACAACTACTA CGAGTTCAAC GCCCACATCT 
TCTACAGAAC CTAGGTCAAC CTCACCCCAG GAAGATATAC AGGTACCCAA GCCTATCACC 
AATGAAGAAT TTCAGGCTAT GATTCCTGCT CATTTCCTTC GTCCTCCTAC TTCCTCACCA 
TCGCCAGATT CCCATCAAGG CCCTATCGTG ACAGTGACAA TAAAACAGCC TGATAATCTA 
CCTGGAGATG TTAATTTTCC TCCGGCTCCT ACAACACTTG GTAAAGTTAC TGAGACCATC 
ACAAAGAGCA CTCTCACCGA GACCGTCGTA ACTAGGGTGA CTGAAAATCA ATTGGTACCA 
CCAGTAATTA TTGAGGACGT GATTCTTGTA AAAGAGGGAT CTCTAGGATT TAGTATTATT 
GGTGGTACGG ATCATTCATG CACTCCATTT GGTGCAAAGG AACCTGGAAT ATTTATATCT 
CATGTCGTGC CTGGTGGTAT AGCTGCAAAA TCTGGTAAAT TAAGAATGGG TGATAGAATA 
CTAAAGGTAA ATGGTACTGA TGTAACAAAA GCTACTCATC AAGAGGCTGT GATGGAACTT 
TTGCGACCAG GAGATCAAAT AGTGCTTACT GTCCAACACG ATCCACTACC CGAAAATTAT 
CAGCTGGTCG AAATAGAGTA CATCCCTGTG ACAGAATTAG TTATTACAAA AGAACCAGGA 
GAGAAACTGG GCATGCATAT TAAAGGTGGA CTTCGAGGAC AAAAAGGTAA TCCACTTGAT 
CATACAGATG AAGGGGTTTT TATATCCAAA ATCAACTCAG GTGGTGCAGC TAAAAGAGAT 
GGAAGACTGA AAGTTGGAAT GAGACTATTA GAAGTTAATG GTACATCATT ATTAGGCGCA 
ACTCACCAAG AAGCAGTAAA TATATTGCGA TGTTCAGGAA ATACGATTAC TTTGGTGGTT 
TGTAAAGGAT ATGATAAGAG TGAAATTGAA CCAGTATTAC CAATATCCGA TGGAAGAGAT 
TCTAAGGAAT CTAGAACATC CAAAGAATTA AAGGATGCTA CAACAGAAGG TACTAAATCG 
TTATCACAAA GTATATCCAG TCTGGATCGC GATGATGAAG AAGCAGCAAC TCTTAGACAA 
GAACAGGAAA TGAAAGCTGA ACTTGTCGCA TGGGAACAAG AAGAAAGAGA ACGTGCTTTG 
CTTGAGCATA GAGAAAAATC TACTCCTGAA AAAGTATTAG ATGTAGTAAG AGCAGCTGAA 
TCATTAGTAA GCAAATCAAA TAGTCCGGTT GATATGGTCG TACCACCAAA GTCACCAGGT 
GGTACGAAAG ATCTCAAAAC AACCACTATT GTGATGAGCA AACATACTCT AGCACCTCAA 
AATACAAATT CACTGAGGAA AGAGAGAACT GGAACATCAG TGGACAATCC ATCGCCCAAG 
TCTCCGATCT CTACTTTAAC TCCATCGATG AATTTCGATC ACACTACTGC TGTCCAAAGC 
TTGCCCTCTC CAAAGAAAAA AAGTTCCATA CCAAAACGTA GTATTACATT TGCTGATCTT 
CCTCCCATTT CTAAAGAACC AATTAATTAT TCCATTTCGG CGAAAGAAGA TCCATCACCT 
TTGCATGATA GTAAATTTAC TTTCGATCCT CAAACTATTT CTTATAAGAA AGTCTATCAA 
AATCCTATCC AAGCTACTCA TTCTCGTTTC AAACTTCCTC CTACACCTTT AACTGCTCCT 
AAATCTATAG AAGATTATGG TACTTCAATT TCTTTTGGTA AACGACAGAA CGCACGCTCT 
GTTGATGGGA ATAGTCAGGT TGAGCTTTCT CCAACAACAT CACCAACACC ACCTCTAGTA 
AAGATGTCGG TTAGTGATAA AAAAAAATTG TTTGAAAGTG CCATGGAGGA ACATTTAAAA 
CCTTCCCCTA AACCAGAAAA AGTATTCAGT TTTCTAAGTC AGGATGAAGT TGAGAAAATG 
AAACAAGAAG AAGAAAAGAA GATTGCTACT CTAACGCGGG ATGAATTAAA GTCGTGGGCA 
CAACTTGATG AAAATGAAGG TTTGGAAGAT TTAGAAGAAA CATTAGAAGA TCAGGATAAT 
CGGCGACCTA ATAGCCGACT GAGTTCGCGA ACTTCGATCC CTCTTCTGCA AAATCTTCCA 
AGCAGTGTAA GGACAGCAAA AGCAGAACGC CGCTTGAAAG AAAGATTAAT ACAGGAGGGT 
ATAATTTCGG ATGAAGATGA AGAAAGTCAT TTAAGTCCTG CCGAACAGAG AGCGTTAAGA 
GCAGAAAAAC GTGCGGCATG GAGACAAGCA CGTTTGAAAT CTCTGGAACA AGATGCTCTT 
CAAGCACAGA TAGTAATTAA AAAAATGAGT GAAATGATGG ACACGAATAA AACAAGTTCA 
ATGCAGGATT CTACAGATAT TCCTATTACT CCTGTTTCTG AATCGGAAAA GACAACTGAT 
TTCACTACGT TACGGCCGTC TAGTGCAGAT TTTCCCAAAC TTGCTGTACG TAGCAAAGTG 
GGTCCCCCTA AAGAGATACG CGAATCCGAG AAAGTAGTGG ACGAGAAGGT CACTCGACGT 
ACTGAGGAAT ATGTGGATGA GGTGACGGGT GAACGACGTG TGCGAACAGT CGAATACGTC 
GAGAAACTCA TTGAACGACA GGTTGAAACT CTACGAGAAA AGATAATATC ATTGGAATTA 
AGTAATGCAG AAGATGAAAT AGAAAGTATT ACCGGTACAG GAGCAAGTGA TGCTGAAAGC 
GAAAGCGAAG AAATTACTGT ACAACCTCCT ACTATGACTA CTTTACAAGA AAGAAATGAA 
ACAATTATAT CTACAAATGA AGGTGCCGCA CCTAAAATAA GTGCAAATAC TGTTCTTGTT 
TCAAAAAAAA AGAAACGAAA ACGTTCGAAG AAAGGTCGTC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108003564 ( XM_017065887.2 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_016921376.1 Apis cerana protein lap4 (LOC108003564), transcript variant X1, mRNA.

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CloneID	OAp29721
Clone ID Related Accession (Same CDS sequence)	XM_017065887.1 , XM_017065887.2
Accession Version	XM_017065887.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-16
Organism	Apis cerana(Asiatic honeybee)
Product	protein lap4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016017567.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 17, 2019 this sequence version replaced XM_017065887.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis cerana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGC AGATATCATC GAGAAAAAAC 
TCAGTGTGCT CTAGTTTAAG TACTAGTAGA GCTCCAAGTG CAACATCTCA TGTTTCATCT 
ATAGGTTTTT CACATAATTT AGAAAATGGT GATGCTTTAT CTCATGATGC TATTAAGTGT 
AAGAAAATTC CTGAACCTAT ATCTTCAACA AAAGTAGGCA ATGAACCAAT GGTATCAGTT 
CTTGTTCACA CAACATTAAT ACGGGACCAA AATGGGCTTG GATTTAGTAT TGCTGGTGGA 
GAAGGTTCTC CACCATTCAA AGACAATAGT GATGCGATAT ATATTTCAAG AATAACAGAT 
GGCGGCGTAG CACAAAAAGA TGGTAAATTA TTAGTTGGAG ACAAAGTAAT ATCTATTAAT 
GGAGTTGAGA TGAGAGGAGC TAAGCATGAA CAAGCAGTTG CTTTATTAAC AGGTTTAGAA 
AGATTTGTTC GATTAGTAGT TGAACGCGAA ATACCATTTT CGCAAGCAAA TGCAGCTACG 
GTCGTAACAC CTTCAGAAAA ATCACCACGA GTGATCGGTG CACCTAAACC ATATACAGGA 
TTATATAATG CCAATAGTTA TATAGCAAAT AAACCGGGAT ATACTGGATA CAGACGTTCT 
ATAGATGCTG ATAGAACATT ATCTCCAAGC CCTACTTCGA AAACGCCTCC ACCTATGAAA 
ATTGAAACTA CTGAATTACC GAAAATGAAC GGTGTTACAG AATCAGGAAA TATTAAAAAT 
GTTTCTTCGA TATCACCAAG TCCAACAAAT AGCCAACTAC ATCCACAGCC TGCTCCTAGG 
CACAGCATTT CGCAACCAAC AATTACACCT ACAACTACTA CGAGTTCAAC GCCCACATCT 
TCTACAGAAC CTAGGTCAAC CTCACCCCAG GAAGATATAC AGGTACCCAA GCCTATCACC 
AATGAAGAAT TTCAGGCTAT GATTCCTGCT CATTTCCTTC GTCCTCCTAC TTCCTCACCA 
TCGCCAGATT CCCATCAAGG CCCTATCGTG ACAGTGACAA TAAAACAGCC TGATAATCTA 
CCTGGAGATG TTAATTTTCC TCCGGCTCCT ACAACACTTG GTAAAGTTAC TGAGACCATC 
ACAAAGAGCA CTCTCACCGA GACCGTCGTA ACTAGGGTGA CTGAAAATCA ATTGGTACCA 
CCAGTAATTA TTGAGGACGT GATTCTTGTA AAAGAGGGAT CTCTAGGATT TAGTATTATT 
GGTGGTACGG ATCATTCATG CACTCCATTT GGTGCAAAGG AACCTGGAAT ATTTATATCT 
CATGTCGTGC CTGGTGGTAT AGCTGCAAAA TCTGGTAAAT TAAGAATGGG TGATAGAATA 
CTAAAGGTAA ATGGTACTGA TGTAACAAAA GCTACTCATC AAGAGGCTGT GATGGAACTT 
TTGCGACCAG GAGATCAAAT AGTGCTTACT GTCCAACACG ATCCACTACC CGAAAATTAT 
CAGCTGGTCG AAATAGAGTA CATCCCTGTG ACAGAATTAG TTATTACAAA AGAACCAGGA 
GAGAAACTGG GCATGCATAT TAAAGGTGGA CTTCGAGGAC AAAAAGGTAA TCCACTTGAT 
CATACAGATG AAGGGGTTTT TATATCCAAA ATCAACTCAG GTGGTGCAGC TAAAAGAGAT 
GGAAGACTGA AAGTTGGAAT GAGACTATTA GAAGTTAATG GTACATCATT ATTAGGCGCA 
ACTCACCAAG AAGCAGTAAA TATATTGCGA TGTTCAGGAA ATACGATTAC TTTGGTGGTT 
TGTAAAGGAT ATGATAAGAG TGAAATTGAA CCAGTATTAC CAATATCCGA TGGAAGAGAT 
TCTAAGGAAT CTAGAACATC CAAAGAATTA AAGGATGCTA CAACAGAAGG TACTAAATCG 
TTATCACAAA GTATATCCAG TCTGGATCGC GATGATGAAG AAGCAGCAAC TCTTAGACAA 
GAACAGGAAA TGAAAGCTGA ACTTGTCGCA TGGGAACAAG AAGAAAGAGA ACGTGCTTTG 
CTTGAGCATA GAGAAAAATC TACTCCTGAA AAAGTATTAG ATGTAGTAAG AGCAGCTGAA 
TCATTAGTAA GCAAATCAAA TAGTCCGGTT GATATGGTCG TACCACCAAA GTCACCAGGT 
GGTACGAAAG ATCTCAAAAC AACCACTATT GTGATGAGCA AACATACTCT AGCACCTCAA 
AATACAAATT CACTGAGGAA AGAGAGAACT GGAACATCAG TGGACAATCC ATCGCCCAAG 
TCTCCGATCT CTACTTTAAC TCCATCGATG AATTTCGATC ACACTACTGC TGTCCAAAGC 
TTGCCCTCTC CAAAGAAAAA AAGTTCCATA CCAAAACGTA GTATTACATT TGCTGATCTT 
CCTCCCATTT CTAAAGAACC AATTAATTAT TCCATTTCGG CGAAAGAAGA TCCATCACCT 
TTGCATGATA GTAAATTTAC TTTCGATCCT CAAACTATTT CTTATAAGAA AGTCTATCAA 
AATCCTATCC AAGCTACTCA TTCTCGTTTC AAACTTCCTC CTACACCTTT AACTGCTCCT 
AAATCTATAG AAGATTATGG TACTTCAATT TCTTTTGGTA AACGACAGAA CGCACGCTCT 
GTTGATGGGA ATAGTCAGGT TGAGCTTTCT CCAACAACAT CACCAACACC ACCTCTAGTA 
AAGATGTCGG TTAGTGATAA AAAAAAATTG TTTGAAAGTG CCATGGAGGA ACATTTAAAA 
CCTTCCCCTA AACCAGAAAA AGTATTCAGT TTTCTAAGTC AGGATGAAGT TGAGAAAATG 
AAACAAGAAG AAGAAAAGAA GATTGCTACT CTAACGCGGG ATGAATTAAA GTCGTGGGCA 
CAACTTGATG AAAATGAAGG TTTGGAAGAT TTAGAAGAAA CATTAGAAGA TCAGGATAAT 
CGGCGACCTA ATAGCCGACT GAGTTCGCGA ACTTCGATCC CTCTTCTGCA AAATCTTCCA 
AGCAGTGTAA GGACAGCAAA AGCAGAACGC CGCTTGAAAG AAAGATTAAT ACAGGAGGGT 
ATAATTTCGG ATGAAGATGA AGAAAGTCAT TTAAGTCCTG CCGAACAGAG AGCGTTAAGA 
GCAGAAAAAC GTGCGGCATG GAGACAAGCA CGTTTGAAAT CTCTGGAACA AGATGCTCTT 
CAAGCACAGA TAGTAATTAA AAAAATGAGT GAAATGATGG ACACGAATAA AACAAGTTCA 
ATGCAGGATT CTACAGATAT TCCTATTACT CCTGTTTCTG AATCGGAAAA GACAACTGAT 
TTCACTACGT TACGGCCGTC TAGTGCAGAT TTTCCCAAAC TTGCTGTACG TAGCAAAGTG 
GGTCCCCCTA AAGAGATACG CGAATCCGAG AAAGTAGTGG ACGAGAAGGT CACTCGACGT 
ACTGAGGAAT ATGTGGATGA GGTGACGGGT GAACGACGTG TGCGAACAGT CGAATACGTC 
GAGAAACTCA TTGAACGACA GGTTGAAACT CTACGAGAAA AGATAATATC ATTGGAATTA 
AGTAATGCAG AAGATGAAAT AGAAAGTATT ACCGGTACAG GAGCAAGTGA TGCTGAAAGC 
GAAAGCGAAG AAATTACTGT ACAACCTCCT ACTATGACTA CTTTACAAGA AAGAAATGAA 
ACAATTATAT CTACAAATGA AGGTGCCGCA CCTAAAATAA GTGCAAATAC TGTTCTTGTT 
TCAAAAAAAA AGAAACGAAA ACGTTCGAAG AAAGGTCGTC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016921376.1
CDS	1476..7640
Translation	MFRCIPIFKGCNRQVEYIDKRHCSLPCVPDDILRYSRSLEELLLDANHIRDLPKNFFRLQKLRKLGLSDNEIHRLPPDIQNFENLVELDVSRNDIPDIPENIKNLRALQVADFSSNPIPRLPAGFVQLRNLTVLGLNDMSLTNLPPDFGSLEALQSLELRENLLKSLPESLSQLYKLERLDLGDNDIEVLPAHIGELPALQELWLDHNQLQHLPPEIGELKTLVCLDVSENRLEDLPEEIGGLEALTDLHLSQNVIEKLPDGLGELKKLTILKVDQNRLSTLNPNIGRCENLQELILTENFLLELPVSIGKLLNLNNLNVDRNSLQSLPTETGNLKQLGVLSLRDNKLQYLPIEVGQCTALHVLDVSGNRLQYLPYSLINLNLKAVWLSKNQAQPMLTFQTDVDEETGQEVLTCFLLPQLEIHPDDSGRLGMLAGMRNAVQDGEIRELGSSDDEGWQEKEASRTHSVKFTDEPPETDKETPFVRQNTPHPKELKAKAHKLFSKGKNDSRSASTDEQDVSQTFGLDKTETDVSTKPNDLTVDLIEQESLKHESVENEQKEIVPGVTDNIDVQSSNEPEIVSNQYIVESNTDVRTEGSISELKDRNDKEDDESENEETQRHVEFSIAEGTDYDGSGDSNRPNRLHRRDTPHHLKNKRIHTAVDKEKVASIIAQALMKKNDEISTSTSAPAETTENFDAQSQDAISDAEPTIEVREEQYEIHIERTTGGLGLSIAGGIGSTPFKGDDEGIFISRVTEGGPADLAGLKVEDKVLSVNGVSVVNVGHYDAVEVLKACGRVLVLVVQREVTRIVPPFEQQISSRKNSVCSSLSTSRAPSATSHVSSIGFSHNLENGDALSHDAIKCKKIPEPISSTKVGNEPMVSVLVHTTLIRDQNGLGFSIAGGEGSPPFKDNSDAIYISRITDGGVAQKDGKLLVGDKVISINGVEMRGAKHEQAVALLTGLERFVRLVVEREIPFSQANAATVVTPSEKSPRVIGAPKPYTGLYNANSYIANKPGYTGYRRSIDADRTLSPSPTSKTPPPMKIETTELPKMNGVTESGNIKNVSSISPSPTNSQLHPQPAPRHSISQPTITPTTTTSSTPTSSTEPRSTSPQEDIQVPKPITNEEFQAMIPAHFLRPPTSSPSPDSHQGPIVTVTIKQPDNLPGDVNFPPAPTTLGKVTETITKSTLTETVVTRVTENQLVPPVIIEDVILVKEGSLGFSIIGGTDHSCTPFGAKEPGIFISHVVPGGIAAKSGKLRMGDRILKVNGTDVTKATHQEAVMELLRPGDQIVLTVQHDPLPENYQLVEIEYIPVTELVITKEPGEKLGMHIKGGLRGQKGNPLDHTDEGVFISKINSGGAAKRDGRLKVGMRLLEVNGTSLLGATHQEAVNILRCSGNTITLVVCKGYDKSEIEPVLPISDGRDSKESRTSKELKDATTEGTKSLSQSISSLDRDDEEAATLRQEQEMKAELVAWEQEERERALLEHREKSTPEKVLDVVRAAESLVSKSNSPVDMVVPPKSPGGTKDLKTTTIVMSKHTLAPQNTNSLRKERTGTSVDNPSPKSPISTLTPSMNFDHTTAVQSLPSPKKKSSIPKRSITFADLPPISKEPINYSISAKEDPSPLHDSKFTFDPQTISYKKVYQNPIQATHSRFKLPPTPLTAPKSIEDYGTSISFGKRQNARSVDGNSQVELSPTTSPTPPLVKMSVSDKKKLFESAMEEHLKPSPKPEKVFSFLSQDEVEKMKQEEEKKIATLTRDELKSWAQLDENEGLEDLEETLEDQDNRRPNSRLSSRTSIPLLQNLPSSVRTAKAERRLKERLIQEGIISDEDEESHLSPAEQRALRAEKRAAWRQARLKSLEQDALQAQIVIKKMSEMMDTNKTSSMQDSTDIPITPVSESEKTTDFTTLRPSSADFPKLAVRSKVGPPKEIRESEKVVDEKVTRRTEEYVDEVTGERRVRTVEYVEKLIERQVETLREKIISLELSNAEDEIESITGTGASDAESESEEITVQPPTMTTLQERNETIISTNEGAAPKISANTVLVSKKKKRKRSKKGRH

Target ORF information:

RefSeq Version	XM_017065887.2
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana protein lap4 (LOC108003564), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017065887.2

ORF Insert Sequence:

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGC AGATATCATC GAGAAAAAAC 
TCAGTGTGCT CTAGTTTAAG TACTAGTAGA GCTCCAAGTG CAACATCTCA TGTTTCATCT 
ATAGGTTTTT CACATAATTT AGAAAATGGT GATGCTTTAT CTCATGATGC TATTAAGTGT 
AAGAAAATTC CTGAACCTAT ATCTTCAACA AAAGTAGGCA ATGAACCAAT GGTATCAGTT 
CTTGTTCACA CAACATTAAT ACGGGACCAA AATGGGCTTG GATTTAGTAT TGCTGGTGGA 
GAAGGTTCTC CACCATTCAA AGACAATAGT GATGCGATAT ATATTTCAAG AATAACAGAT 
GGCGGCGTAG CACAAAAAGA TGGTAAATTA TTAGTTGGAG ACAAAGTAAT ATCTATTAAT 
GGAGTTGAGA TGAGAGGAGC TAAGCATGAA CAAGCAGTTG CTTTATTAAC AGGTTTAGAA 
AGATTTGTTC GATTAGTAGT TGAACGCGAA ATACCATTTT CGCAAGCAAA TGCAGCTACG 
GTCGTAACAC CTTCAGAAAA ATCACCACGA GTGATCGGTG CACCTAAACC ATATACAGGA 
TTATATAATG CCAATAGTTA TATAGCAAAT AAACCGGGAT ATACTGGATA CAGACGTTCT 
ATAGATGCTG ATAGAACATT ATCTCCAAGC CCTACTTCGA AAACGCCTCC ACCTATGAAA 
ATTGAAACTA CTGAATTACC GAAAATGAAC GGTGTTACAG AATCAGGAAA TATTAAAAAT 
GTTTCTTCGA TATCACCAAG TCCAACAAAT AGCCAACTAC ATCCACAGCC TGCTCCTAGG 
CACAGCATTT CGCAACCAAC AATTACACCT ACAACTACTA CGAGTTCAAC GCCCACATCT 
TCTACAGAAC CTAGGTCAAC CTCACCCCAG GAAGATATAC AGGTACCCAA GCCTATCACC 
AATGAAGAAT TTCAGGCTAT GATTCCTGCT CATTTCCTTC GTCCTCCTAC TTCCTCACCA 
TCGCCAGATT CCCATCAAGG CCCTATCGTG ACAGTGACAA TAAAACAGCC TGATAATCTA 
CCTGGAGATG TTAATTTTCC TCCGGCTCCT ACAACACTTG GTAAAGTTAC TGAGACCATC 
ACAAAGAGCA CTCTCACCGA GACCGTCGTA ACTAGGGTGA CTGAAAATCA ATTGGTACCA 
CCAGTAATTA TTGAGGACGT GATTCTTGTA AAAGAGGGAT CTCTAGGATT TAGTATTATT 
GGTGGTACGG ATCATTCATG CACTCCATTT GGTGCAAAGG AACCTGGAAT ATTTATATCT 
CATGTCGTGC CTGGTGGTAT AGCTGCAAAA TCTGGTAAAT TAAGAATGGG TGATAGAATA 
CTAAAGGTAA ATGGTACTGA TGTAACAAAA GCTACTCATC AAGAGGCTGT GATGGAACTT 
TTGCGACCAG GAGATCAAAT AGTGCTTACT GTCCAACACG ATCCACTACC CGAAAATTAT 
CAGCTGGTCG AAATAGAGTA CATCCCTGTG ACAGAATTAG TTATTACAAA AGAACCAGGA 
GAGAAACTGG GCATGCATAT TAAAGGTGGA CTTCGAGGAC AAAAAGGTAA TCCACTTGAT 
CATACAGATG AAGGGGTTTT TATATCCAAA ATCAACTCAG GTGGTGCAGC TAAAAGAGAT 
GGAAGACTGA AAGTTGGAAT GAGACTATTA GAAGTTAATG GTACATCATT ATTAGGCGCA 
ACTCACCAAG AAGCAGTAAA TATATTGCGA TGTTCAGGAA ATACGATTAC TTTGGTGGTT 
TGTAAAGGAT ATGATAAGAG TGAAATTGAA CCAGTATTAC CAATATCCGA TGGAAGAGAT 
TCTAAGGAAT CTAGAACATC CAAAGAATTA AAGGATGCTA CAACAGAAGG TACTAAATCG 
TTATCACAAA GTATATCCAG TCTGGATCGC GATGATGAAG AAGCAGCAAC TCTTAGACAA 
GAACAGGAAA TGAAAGCTGA ACTTGTCGCA TGGGAACAAG AAGAAAGAGA ACGTGCTTTG 
CTTGAGCATA GAGAAAAATC TACTCCTGAA AAAGTATTAG ATGTAGTAAG AGCAGCTGAA 
TCATTAGTAA GCAAATCAAA TAGTCCGGTT GATATGGTCG TACCACCAAA GTCACCAGGT 
GGTACGAAAG ATCTCAAAAC AACCACTATT GTGATGAGCA AACATACTCT AGCACCTCAA 
AATACAAATT CACTGAGGAA AGAGAGAACT GGAACATCAG TGGACAATCC ATCGCCCAAG 
TCTCCGATCT CTACTTTAAC TCCATCGATG AATTTCGATC ACACTACTGC TGTCCAAAGC 
TTGCCCTCTC CAAAGAAAAA AAGTTCCATA CCAAAACGTA GTATTACATT TGCTGATCTT 
CCTCCCATTT CTAAAGAACC AATTAATTAT TCCATTTCGG CGAAAGAAGA TCCATCACCT 
TTGCATGATA GTAAATTTAC TTTCGATCCT CAAACTATTT CTTATAAGAA AGTCTATCAA 
AATCCTATCC AAGCTACTCA TTCTCGTTTC AAACTTCCTC CTACACCTTT AACTGCTCCT 
AAATCTATAG AAGATTATGG TACTTCAATT TCTTTTGGTA AACGACAGAA CGCACGCTCT 
GTTGATGGGA ATAGTCAGGT TGAGCTTTCT CCAACAACAT CACCAACACC ACCTCTAGTA 
AAGATGTCGG TTAGTGATAA AAAAAAATTG TTTGAAAGTG CCATGGAGGA ACATTTAAAA 
CCTTCCCCTA AACCAGAAAA AGTATTCAGT TTTCTAAGTC AGGATGAAGT TGAGAAAATG 
AAACAAGAAG AAGAAAAGAA GATTGCTACT CTAACGCGGG ATGAATTAAA GTCGTGGGCA 
CAACTTGATG AAAATGAAGG TTTGGAAGAT TTAGAAGAAA CATTAGAAGA TCAGGATAAT 
CGGCGACCTA ATAGCCGACT GAGTTCGCGA ACTTCGATCC CTCTTCTGCA AAATCTTCCA 
AGCAGTGTAA GGACAGCAAA AGCAGAACGC CGCTTGAAAG AAAGATTAAT ACAGGAGGGT 
ATAATTTCGG ATGAAGATGA AGAAAGTCAT TTAAGTCCTG CCGAACAGAG AGCGTTAAGA 
GCAGAAAAAC GTGCGGCATG GAGACAAGCA CGTTTGAAAT CTCTGGAACA AGATGCTCTT 
CAAGCACAGA TAGTAATTAA AAAAATGAGT GAAATGATGG ACACGAATAA AACAAGTTCA 
ATGCAGGATT CTACAGATAT TCCTATTACT CCTGTTTCTG AATCGGAAAA GACAACTGAT 
TTCACTACGT TACGGCCGTC TAGTGCAGAT TTTCCCAAAC TTGCTGTACG TAGCAAAGTG 
GGTCCCCCTA AAGAGATACG CGAATCCGAG AAAGTAGTGG ACGAGAAGGT CACTCGACGT 
ACTGAGGAAT ATGTGGATGA GGTGACGGGT GAACGACGTG TGCGAACAGT CGAATACGTC 
GAGAAACTCA TTGAACGACA GGTTGAAACT CTACGAGAAA AGATAATATC ATTGGAATTA 
AGTAATGCAG AAGATGAAAT AGAAAGTATT ACCGGTACAG GAGCAAGTGA TGCTGAAAGC 
GAAAGCGAAG AAATTACTGT ACAACCTCCT ACTATGACTA CTTTACAAGA AAGAAATGAA 
ACAATTATAT CTACAAATGA AGGTGCCGCA CCTAAAATAA GTGCAAATAC TGTTCTTGTT 
TCAAAAAAAA AGAAACGAAA ACGTTCGAAG AAAGGTCGTC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108003564 ( XM_017065888.1 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_016921377.1 Apis cerana protein lap4 (LOC108003564), transcript variant X2, mRNA.

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CloneID	OAp29722
Clone ID Related Accession (Same CDS sequence)	XM_017065888.1 , XM_017065888.2
Accession Version	XM_017065888.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-08
Organism	Apis cerana(Asiatic honeybee)
Product	protein lap4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016017567.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis cerana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGA TATCATCGAG AAAAAACTCA 
GTGTGCTCTA GTTTAAGTAC TAGTAGAGCT CCAAGTGCAA CATCTCATGT TTCATCTATA 
GGTTTTTCAC ATAATTTAGA AAATGGTGAT GCTTTATCTC ATGATGCTAT TAAGTGTAAG 
AAAATTCCTG AACCTATATC TTCAACAAAA GTAGGCAATG AACCAATGGT ATCAGTTCTT 
GTTCACACAA CATTAATACG GGACCAAAAT GGGCTTGGAT TTAGTATTGC TGGTGGAGAA 
GGTTCTCCAC CATTCAAAGA CAATAGTGAT GCGATATATA TTTCAAGAAT AACAGATGGC 
GGCGTAGCAC AAAAAGATGG TAAATTATTA GTTGGAGACA AAGTAATATC TATTAATGGA 
GTTGAGATGA GAGGAGCTAA GCATGAACAA GCAGTTGCTT TATTAACAGG TTTAGAAAGA 
TTTGTTCGAT TAGTAGTTGA ACGCGAAATA CCATTTTCGC AAGCAAATGC AGCTACGGTC 
GTAACACCTT CAGAAAAATC ACCACGAGTG ATCGGTGCAC CTAAACCATA TACAGGATTA 
TATAATGCCA ATAGTTATAT AGCAAATAAA CCGGGATATA CTGGATACAG ACGTTCTATA 
GATGCTGATA GAACATTATC TCCAAGCCCT ACTTCGAAAA CGCCTCCACC TATGAAAATT 
GAAACTACTG AATTACCGAA AATGAACGGT GTTACAGAAT CAGGAAATAT TAAAAATGTT 
TCTTCGATAT CACCAAGTCC AACAAATAGC CAACTACATC CACAGCCTGC TCCTAGGCAC 
AGCATTTCGC AACCAACAAT TACACCTACA ACTACTACGA GTTCAACGCC CACATCTTCT 
ACAGAACCTA GGTCAACCTC ACCCCAGGAA GATATACAGG TACCCAAGCC TATCACCAAT 
GAAGAATTTC AGGCTATGAT TCCTGCTCAT TTCCTTCGTC CTCCTACTTC CTCACCATCG 
CCAGATTCCC ATCAAGGCCC TATCGTGACA GTGACAATAA AACAGCCTGA TAATCTACCT 
GGAGATGTTA ATTTTCCTCC GGCTCCTACA ACACTTGGTA AAGTTACTGA GACCATCACA 
AAGAGCACTC TCACCGAGAC CGTCGTAACT AGGGTGACTG AAAATCAATT GGTACCACCA 
GTAATTATTG AGGACGTGAT TCTTGTAAAA GAGGGATCTC TAGGATTTAG TATTATTGGT 
GGTACGGATC ATTCATGCAC TCCATTTGGT GCAAAGGAAC CTGGAATATT TATATCTCAT 
GTCGTGCCTG GTGGTATAGC TGCAAAATCT GGTAAATTAA GAATGGGTGA TAGAATACTA 
AAGGTAAATG GTACTGATGT AACAAAAGCT ACTCATCAAG AGGCTGTGAT GGAACTTTTG 
CGACCAGGAG ATCAAATAGT GCTTACTGTC CAACACGATC CACTACCCGA AAATTATCAG 
CTGGTCGAAA TAGAGTACAT CCCTGTGACA GAATTAGTTA TTACAAAAGA ACCAGGAGAG 
AAACTGGGCA TGCATATTAA AGGTGGACTT CGAGGACAAA AAGGTAATCC ACTTGATCAT 
ACAGATGAAG GGGTTTTTAT ATCCAAAATC AACTCAGGTG GTGCAGCTAA AAGAGATGGA 
AGACTGAAAG TTGGAATGAG ACTATTAGAA GTTAATGGTA CATCATTATT AGGCGCAACT 
CACCAAGAAG CAGTAAATAT ATTGCGATGT TCAGGAAATA CGATTACTTT GGTGGTTTGT 
AAAGGATATG ATAAGAGTGA AATTGAACCA GTATTACCAA TATCCGATGG AAGAGATTCT 
AAGGAATCTA GAACATCCAA AGAATTAAAG GATGCTACAA CAGAAGGTAC TAAATCGTTA 
TCACAAAGTA TATCCAGTCT GGATCGCGAT GATGAAGAAG CAGCAACTCT TAGACAAGAA 
CAGGAAATGA AAGCTGAACT TGTCGCATGG GAACAAGAAG AAAGAGAACG TGCTTTGCTT 
GAGCATAGAG AAAAATCTAC TCCTGAAAAA GTATTAGATG TAGTAAGAGC AGCTGAATCA 
TTAGTAAGCA AATCAAATAG TCCGGTTGAT ATGGTCGTAC CACCAAAGTC ACCAGGTGGT 
ACGAAAGATC TCAAAACAAC CACTATTGTG ATGAGCAAAC ATACTCTAGC ACCTCAAAAT 
ACAAATTCAC TGAGGAAAGA GAGAACTGGA ACATCAGTGG ACAATCCATC GCCCAAGTCT 
CCGATCTCTA CTTTAACTCC ATCGATGAAT TTCGATCACA CTACTGCTGT CCAAAGCTTG 
CCCTCTCCAA AGAAAAAAAG TTCCATACCA AAACGTAGTA TTACATTTGC TGATCTTCCT 
CCCATTTCTA AAGAACCAAT TAATTATTCC ATTTCGGCGA AAGAAGATCC ATCACCTTTG 
CATGATAGTA AATTTACTTT CGATCCTCAA ACTATTTCTT ATAAGAAAGT CTATCAAAAT 
CCTATCCAAG CTACTCATTC TCGTTTCAAA CTTCCTCCTA CACCTTTAAC TGCTCCTAAA 
TCTATAGAAG ATTATGGTAC TTCAATTTCT TTTGGTAAAC GACAGAACGC ACGCTCTGTT 
GATGGGAATA GTCAGGTTGA GCTTTCTCCA ACAACATCAC CAACACCACC TCTAGTAAAG 
ATGTCGGTTA GTGATAAAAA AAAATTGTTT GAAAGTGCCA TGGAGGAACA TTTAAAACCT 
TCCCCTAAAC CAGAAAAAGT ATTCAGTTTT CTAAGTCAGG ATGAAGTTGA GAAAATGAAA 
CAAGAAGAAG AAAAGAAGAT TGCTACTCTA ACGCGGGATG AATTAAAGTC GTGGGCACAA 
CTTGATGAAA ATGAAGGTTT GGAAGATTTA GAAGAAACAT TAGAAGATCA GGATAATCGG 
CGACCTAATA GCCGACTGAG TTCGCGAACT TCGATCCCTC TTCTGCAAAA TCTTCCAAGC 
AGTGTAAGGA CAGCAAAAGC AGAACGCCGC TTGAAAGAAA GATTAATACA GGAGGGTATA 
ATTTCGGATG AAGATGAAGA AAGTCATTTA AGTCCTGCCG AACAGAGAGC GTTAAGAGCA 
GAAAAACGTG CGGCATGGAG ACAAGCACGT TTGAAATCTC TGGAACAAGA TGCTCTTCAA 
GCACAGATAG TAATTAAAAA AATGAGTGAA ATGATGGACA CGAATAAAAC AAGTTCAATG 
CAGGATTCTA CAGATATTCC TATTACTCCT GTTTCTGAAT CGGAAAAGAC AACTGATTTC 
ACTACGTTAC GGCCGTCTAG TGCAGATTTT CCCAAACTTG CTGTACGTAG CAAAGTGGGT 
CCCCCTAAAG AGATACGCGA ATCCGAGAAA GTAGTGGACG AGAAGGTCAC TCGACGTACT 
GAGGAATATG TGGATGAGGT GACGGGTGAA CGACGTGTGC GAACAGTCGA ATACGTCGAG 
AAACTCATTG AACGACAGGT TGAAACTCTA CGAGAAAAGA TAATATCATT GGAATTAAGT 
AATGCAGAAG ATGAAATAGA AAGTATTACC GGTACAGGAG CAAGTGATGC TGAAAGCGAA 
AGCGAAGAAA TTACTGTACA ACCTCCTACT ATGACTACTT TACAAGAAAG AAATGAAACA 
ATTATATCTA CAAATGAAGG TGCCGCACCT AAAATAAGTG CAAATACTGT TCTTGTTTCA 
AAAAAAAAGA AACGAAAACG TTCGAAGAAA GGTCGTCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016921377.1
CDS	233..6394
Translation	MFRCIPIFKGCNRQVEYIDKRHCSLPCVPDDILRYSRSLEELLLDANHIRDLPKNFFRLQKLRKLGLSDNEIHRLPPDIQNFENLVELDVSRNDIPDIPENIKNLRALQVADFSSNPIPRLPAGFVQLRNLTVLGLNDMSLTNLPPDFGSLEALQSLELRENLLKSLPESLSQLYKLERLDLGDNDIEVLPAHIGELPALQELWLDHNQLQHLPPEIGELKTLVCLDVSENRLEDLPEEIGGLEALTDLHLSQNVIEKLPDGLGELKKLTILKVDQNRLSTLNPNIGRCENLQELILTENFLLELPVSIGKLLNLNNLNVDRNSLQSLPTETGNLKQLGVLSLRDNKLQYLPIEVGQCTALHVLDVSGNRLQYLPYSLINLNLKAVWLSKNQAQPMLTFQTDVDEETGQEVLTCFLLPQLEIHPDDSGRLGMLAGMRNAVQDGEIRELGSSDDEGWQEKEASRTHSVKFTDEPPETDKETPFVRQNTPHPKELKAKAHKLFSKGKNDSRSASTDEQDVSQTFGLDKTETDVSTKPNDLTVDLIEQESLKHESVENEQKEIVPGVTDNIDVQSSNEPEIVSNQYIVESNTDVRTEGSISELKDRNDKEDDESENEETQRHVEFSIAEGTDYDGSGDSNRPNRLHRRDTPHHLKNKRIHTAVDKEKVASIIAQALMKKNDEISTSTSAPAETTENFDAQSQDAISDAEPTIEVREEQYEIHIERTTGGLGLSIAGGIGSTPFKGDDEGIFISRVTEGGPADLAGLKVEDKVLSVNGVSVVNVGHYDAVEVLKACGRVLVLVVQREVTRIVPPFEQISSRKNSVCSSLSTSRAPSATSHVSSIGFSHNLENGDALSHDAIKCKKIPEPISSTKVGNEPMVSVLVHTTLIRDQNGLGFSIAGGEGSPPFKDNSDAIYISRITDGGVAQKDGKLLVGDKVISINGVEMRGAKHEQAVALLTGLERFVRLVVEREIPFSQANAATVVTPSEKSPRVIGAPKPYTGLYNANSYIANKPGYTGYRRSIDADRTLSPSPTSKTPPPMKIETTELPKMNGVTESGNIKNVSSISPSPTNSQLHPQPAPRHSISQPTITPTTTTSSTPTSSTEPRSTSPQEDIQVPKPITNEEFQAMIPAHFLRPPTSSPSPDSHQGPIVTVTIKQPDNLPGDVNFPPAPTTLGKVTETITKSTLTETVVTRVTENQLVPPVIIEDVILVKEGSLGFSIIGGTDHSCTPFGAKEPGIFISHVVPGGIAAKSGKLRMGDRILKVNGTDVTKATHQEAVMELLRPGDQIVLTVQHDPLPENYQLVEIEYIPVTELVITKEPGEKLGMHIKGGLRGQKGNPLDHTDEGVFISKINSGGAAKRDGRLKVGMRLLEVNGTSLLGATHQEAVNILRCSGNTITLVVCKGYDKSEIEPVLPISDGRDSKESRTSKELKDATTEGTKSLSQSISSLDRDDEEAATLRQEQEMKAELVAWEQEERERALLEHREKSTPEKVLDVVRAAESLVSKSNSPVDMVVPPKSPGGTKDLKTTTIVMSKHTLAPQNTNSLRKERTGTSVDNPSPKSPISTLTPSMNFDHTTAVQSLPSPKKKSSIPKRSITFADLPPISKEPINYSISAKEDPSPLHDSKFTFDPQTISYKKVYQNPIQATHSRFKLPPTPLTAPKSIEDYGTSISFGKRQNARSVDGNSQVELSPTTSPTPPLVKMSVSDKKKLFESAMEEHLKPSPKPEKVFSFLSQDEVEKMKQEEEKKIATLTRDELKSWAQLDENEGLEDLEETLEDQDNRRPNSRLSSRTSIPLLQNLPSSVRTAKAERRLKERLIQEGIISDEDEESHLSPAEQRALRAEKRAAWRQARLKSLEQDALQAQIVIKKMSEMMDTNKTSSMQDSTDIPITPVSESEKTTDFTTLRPSSADFPKLAVRSKVGPPKEIRESEKVVDEKVTRRTEEYVDEVTGERRVRTVEYVEKLIERQVETLREKIISLELSNAEDEIESITGTGASDAESESEEITVQPPTMTTLQERNETIISTNEGAAPKISANTVLVSKKKKRKRSKKGRH

Target ORF information:

RefSeq Version	XM_017065888.1
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana protein lap4 (LOC108003564), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017065888.1

ORF Insert Sequence:

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGA TATCATCGAG AAAAAACTCA 
GTGTGCTCTA GTTTAAGTAC TAGTAGAGCT CCAAGTGCAA CATCTCATGT TTCATCTATA 
GGTTTTTCAC ATAATTTAGA AAATGGTGAT GCTTTATCTC ATGATGCTAT TAAGTGTAAG 
AAAATTCCTG AACCTATATC TTCAACAAAA GTAGGCAATG AACCAATGGT ATCAGTTCTT 
GTTCACACAA CATTAATACG GGACCAAAAT GGGCTTGGAT TTAGTATTGC TGGTGGAGAA 
GGTTCTCCAC CATTCAAAGA CAATAGTGAT GCGATATATA TTTCAAGAAT AACAGATGGC 
GGCGTAGCAC AAAAAGATGG TAAATTATTA GTTGGAGACA AAGTAATATC TATTAATGGA 
GTTGAGATGA GAGGAGCTAA GCATGAACAA GCAGTTGCTT TATTAACAGG TTTAGAAAGA 
TTTGTTCGAT TAGTAGTTGA ACGCGAAATA CCATTTTCGC AAGCAAATGC AGCTACGGTC 
GTAACACCTT CAGAAAAATC ACCACGAGTG ATCGGTGCAC CTAAACCATA TACAGGATTA 
TATAATGCCA ATAGTTATAT AGCAAATAAA CCGGGATATA CTGGATACAG ACGTTCTATA 
GATGCTGATA GAACATTATC TCCAAGCCCT ACTTCGAAAA CGCCTCCACC TATGAAAATT 
GAAACTACTG AATTACCGAA AATGAACGGT GTTACAGAAT CAGGAAATAT TAAAAATGTT 
TCTTCGATAT CACCAAGTCC AACAAATAGC CAACTACATC CACAGCCTGC TCCTAGGCAC 
AGCATTTCGC AACCAACAAT TACACCTACA ACTACTACGA GTTCAACGCC CACATCTTCT 
ACAGAACCTA GGTCAACCTC ACCCCAGGAA GATATACAGG TACCCAAGCC TATCACCAAT 
GAAGAATTTC AGGCTATGAT TCCTGCTCAT TTCCTTCGTC CTCCTACTTC CTCACCATCG 
CCAGATTCCC ATCAAGGCCC TATCGTGACA GTGACAATAA AACAGCCTGA TAATCTACCT 
GGAGATGTTA ATTTTCCTCC GGCTCCTACA ACACTTGGTA AAGTTACTGA GACCATCACA 
AAGAGCACTC TCACCGAGAC CGTCGTAACT AGGGTGACTG AAAATCAATT GGTACCACCA 
GTAATTATTG AGGACGTGAT TCTTGTAAAA GAGGGATCTC TAGGATTTAG TATTATTGGT 
GGTACGGATC ATTCATGCAC TCCATTTGGT GCAAAGGAAC CTGGAATATT TATATCTCAT 
GTCGTGCCTG GTGGTATAGC TGCAAAATCT GGTAAATTAA GAATGGGTGA TAGAATACTA 
AAGGTAAATG GTACTGATGT AACAAAAGCT ACTCATCAAG AGGCTGTGAT GGAACTTTTG 
CGACCAGGAG ATCAAATAGT GCTTACTGTC CAACACGATC CACTACCCGA AAATTATCAG 
CTGGTCGAAA TAGAGTACAT CCCTGTGACA GAATTAGTTA TTACAAAAGA ACCAGGAGAG 
AAACTGGGCA TGCATATTAA AGGTGGACTT CGAGGACAAA AAGGTAATCC ACTTGATCAT 
ACAGATGAAG GGGTTTTTAT ATCCAAAATC AACTCAGGTG GTGCAGCTAA AAGAGATGGA 
AGACTGAAAG TTGGAATGAG ACTATTAGAA GTTAATGGTA CATCATTATT AGGCGCAACT 
CACCAAGAAG CAGTAAATAT ATTGCGATGT TCAGGAAATA CGATTACTTT GGTGGTTTGT 
AAAGGATATG ATAAGAGTGA AATTGAACCA GTATTACCAA TATCCGATGG AAGAGATTCT 
AAGGAATCTA GAACATCCAA AGAATTAAAG GATGCTACAA CAGAAGGTAC TAAATCGTTA 
TCACAAAGTA TATCCAGTCT GGATCGCGAT GATGAAGAAG CAGCAACTCT TAGACAAGAA 
CAGGAAATGA AAGCTGAACT TGTCGCATGG GAACAAGAAG AAAGAGAACG TGCTTTGCTT 
GAGCATAGAG AAAAATCTAC TCCTGAAAAA GTATTAGATG TAGTAAGAGC AGCTGAATCA 
TTAGTAAGCA AATCAAATAG TCCGGTTGAT ATGGTCGTAC CACCAAAGTC ACCAGGTGGT 
ACGAAAGATC TCAAAACAAC CACTATTGTG ATGAGCAAAC ATACTCTAGC ACCTCAAAAT 
ACAAATTCAC TGAGGAAAGA GAGAACTGGA ACATCAGTGG ACAATCCATC GCCCAAGTCT 
CCGATCTCTA CTTTAACTCC ATCGATGAAT TTCGATCACA CTACTGCTGT CCAAAGCTTG 
CCCTCTCCAA AGAAAAAAAG TTCCATACCA AAACGTAGTA TTACATTTGC TGATCTTCCT 
CCCATTTCTA AAGAACCAAT TAATTATTCC ATTTCGGCGA AAGAAGATCC ATCACCTTTG 
CATGATAGTA AATTTACTTT CGATCCTCAA ACTATTTCTT ATAAGAAAGT CTATCAAAAT 
CCTATCCAAG CTACTCATTC TCGTTTCAAA CTTCCTCCTA CACCTTTAAC TGCTCCTAAA 
TCTATAGAAG ATTATGGTAC TTCAATTTCT TTTGGTAAAC GACAGAACGC ACGCTCTGTT 
GATGGGAATA GTCAGGTTGA GCTTTCTCCA ACAACATCAC CAACACCACC TCTAGTAAAG 
ATGTCGGTTA GTGATAAAAA AAAATTGTTT GAAAGTGCCA TGGAGGAACA TTTAAAACCT 
TCCCCTAAAC CAGAAAAAGT ATTCAGTTTT CTAAGTCAGG ATGAAGTTGA GAAAATGAAA 
CAAGAAGAAG AAAAGAAGAT TGCTACTCTA ACGCGGGATG AATTAAAGTC GTGGGCACAA 
CTTGATGAAA ATGAAGGTTT GGAAGATTTA GAAGAAACAT TAGAAGATCA GGATAATCGG 
CGACCTAATA GCCGACTGAG TTCGCGAACT TCGATCCCTC TTCTGCAAAA TCTTCCAAGC 
AGTGTAAGGA CAGCAAAAGC AGAACGCCGC TTGAAAGAAA GATTAATACA GGAGGGTATA 
ATTTCGGATG AAGATGAAGA AAGTCATTTA AGTCCTGCCG AACAGAGAGC GTTAAGAGCA 
GAAAAACGTG CGGCATGGAG ACAAGCACGT TTGAAATCTC TGGAACAAGA TGCTCTTCAA 
GCACAGATAG TAATTAAAAA AATGAGTGAA ATGATGGACA CGAATAAAAC AAGTTCAATG 
CAGGATTCTA CAGATATTCC TATTACTCCT GTTTCTGAAT CGGAAAAGAC AACTGATTTC 
ACTACGTTAC GGCCGTCTAG TGCAGATTTT CCCAAACTTG CTGTACGTAG CAAAGTGGGT 
CCCCCTAAAG AGATACGCGA ATCCGAGAAA GTAGTGGACG AGAAGGTCAC TCGACGTACT 
GAGGAATATG TGGATGAGGT GACGGGTGAA CGACGTGTGC GAACAGTCGA ATACGTCGAG 
AAACTCATTG AACGACAGGT TGAAACTCTA CGAGAAAAGA TAATATCATT GGAATTAAGT 
AATGCAGAAG ATGAAATAGA AAGTATTACC GGTACAGGAG CAAGTGATGC TGAAAGCGAA 
AGCGAAGAAA TTACTGTACA ACCTCCTACT ATGACTACTT TACAAGAAAG AAATGAAACA 
ATTATATCTA CAAATGAAGG TGCCGCACCT AAAATAAGTG CAAATACTGT TCTTGTTTCA 
AAAAAAAAGA AACGAAAACG TTCGAAGAAA GGTCGTCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108003564 ( XM_017065888.2 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_016921377.1 Apis cerana protein lap4 (LOC108003564), transcript variant X2, mRNA.

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CloneID	OAp29722
Clone ID Related Accession (Same CDS sequence)	XM_017065888.1 , XM_017065888.2
Accession Version	XM_017065888.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-16
Organism	Apis cerana(Asiatic honeybee)
Product	protein lap4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016017567.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 17, 2019 this sequence version replaced XM_017065888.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis cerana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGA TATCATCGAG AAAAAACTCA 
GTGTGCTCTA GTTTAAGTAC TAGTAGAGCT CCAAGTGCAA CATCTCATGT TTCATCTATA 
GGTTTTTCAC ATAATTTAGA AAATGGTGAT GCTTTATCTC ATGATGCTAT TAAGTGTAAG 
AAAATTCCTG AACCTATATC TTCAACAAAA GTAGGCAATG AACCAATGGT ATCAGTTCTT 
GTTCACACAA CATTAATACG GGACCAAAAT GGGCTTGGAT TTAGTATTGC TGGTGGAGAA 
GGTTCTCCAC CATTCAAAGA CAATAGTGAT GCGATATATA TTTCAAGAAT AACAGATGGC 
GGCGTAGCAC AAAAAGATGG TAAATTATTA GTTGGAGACA AAGTAATATC TATTAATGGA 
GTTGAGATGA GAGGAGCTAA GCATGAACAA GCAGTTGCTT TATTAACAGG TTTAGAAAGA 
TTTGTTCGAT TAGTAGTTGA ACGCGAAATA CCATTTTCGC AAGCAAATGC AGCTACGGTC 
GTAACACCTT CAGAAAAATC ACCACGAGTG ATCGGTGCAC CTAAACCATA TACAGGATTA 
TATAATGCCA ATAGTTATAT AGCAAATAAA CCGGGATATA CTGGATACAG ACGTTCTATA 
GATGCTGATA GAACATTATC TCCAAGCCCT ACTTCGAAAA CGCCTCCACC TATGAAAATT 
GAAACTACTG AATTACCGAA AATGAACGGT GTTACAGAAT CAGGAAATAT TAAAAATGTT 
TCTTCGATAT CACCAAGTCC AACAAATAGC CAACTACATC CACAGCCTGC TCCTAGGCAC 
AGCATTTCGC AACCAACAAT TACACCTACA ACTACTACGA GTTCAACGCC CACATCTTCT 
ACAGAACCTA GGTCAACCTC ACCCCAGGAA GATATACAGG TACCCAAGCC TATCACCAAT 
GAAGAATTTC AGGCTATGAT TCCTGCTCAT TTCCTTCGTC CTCCTACTTC CTCACCATCG 
CCAGATTCCC ATCAAGGCCC TATCGTGACA GTGACAATAA AACAGCCTGA TAATCTACCT 
GGAGATGTTA ATTTTCCTCC GGCTCCTACA ACACTTGGTA AAGTTACTGA GACCATCACA 
AAGAGCACTC TCACCGAGAC CGTCGTAACT AGGGTGACTG AAAATCAATT GGTACCACCA 
GTAATTATTG AGGACGTGAT TCTTGTAAAA GAGGGATCTC TAGGATTTAG TATTATTGGT 
GGTACGGATC ATTCATGCAC TCCATTTGGT GCAAAGGAAC CTGGAATATT TATATCTCAT 
GTCGTGCCTG GTGGTATAGC TGCAAAATCT GGTAAATTAA GAATGGGTGA TAGAATACTA 
AAGGTAAATG GTACTGATGT AACAAAAGCT ACTCATCAAG AGGCTGTGAT GGAACTTTTG 
CGACCAGGAG ATCAAATAGT GCTTACTGTC CAACACGATC CACTACCCGA AAATTATCAG 
CTGGTCGAAA TAGAGTACAT CCCTGTGACA GAATTAGTTA TTACAAAAGA ACCAGGAGAG 
AAACTGGGCA TGCATATTAA AGGTGGACTT CGAGGACAAA AAGGTAATCC ACTTGATCAT 
ACAGATGAAG GGGTTTTTAT ATCCAAAATC AACTCAGGTG GTGCAGCTAA AAGAGATGGA 
AGACTGAAAG TTGGAATGAG ACTATTAGAA GTTAATGGTA CATCATTATT AGGCGCAACT 
CACCAAGAAG CAGTAAATAT ATTGCGATGT TCAGGAAATA CGATTACTTT GGTGGTTTGT 
AAAGGATATG ATAAGAGTGA AATTGAACCA GTATTACCAA TATCCGATGG AAGAGATTCT 
AAGGAATCTA GAACATCCAA AGAATTAAAG GATGCTACAA CAGAAGGTAC TAAATCGTTA 
TCACAAAGTA TATCCAGTCT GGATCGCGAT GATGAAGAAG CAGCAACTCT TAGACAAGAA 
CAGGAAATGA AAGCTGAACT TGTCGCATGG GAACAAGAAG AAAGAGAACG TGCTTTGCTT 
GAGCATAGAG AAAAATCTAC TCCTGAAAAA GTATTAGATG TAGTAAGAGC AGCTGAATCA 
TTAGTAAGCA AATCAAATAG TCCGGTTGAT ATGGTCGTAC CACCAAAGTC ACCAGGTGGT 
ACGAAAGATC TCAAAACAAC CACTATTGTG ATGAGCAAAC ATACTCTAGC ACCTCAAAAT 
ACAAATTCAC TGAGGAAAGA GAGAACTGGA ACATCAGTGG ACAATCCATC GCCCAAGTCT 
CCGATCTCTA CTTTAACTCC ATCGATGAAT TTCGATCACA CTACTGCTGT CCAAAGCTTG 
CCCTCTCCAA AGAAAAAAAG TTCCATACCA AAACGTAGTA TTACATTTGC TGATCTTCCT 
CCCATTTCTA AAGAACCAAT TAATTATTCC ATTTCGGCGA AAGAAGATCC ATCACCTTTG 
CATGATAGTA AATTTACTTT CGATCCTCAA ACTATTTCTT ATAAGAAAGT CTATCAAAAT 
CCTATCCAAG CTACTCATTC TCGTTTCAAA CTTCCTCCTA CACCTTTAAC TGCTCCTAAA 
TCTATAGAAG ATTATGGTAC TTCAATTTCT TTTGGTAAAC GACAGAACGC ACGCTCTGTT 
GATGGGAATA GTCAGGTTGA GCTTTCTCCA ACAACATCAC CAACACCACC TCTAGTAAAG 
ATGTCGGTTA GTGATAAAAA AAAATTGTTT GAAAGTGCCA TGGAGGAACA TTTAAAACCT 
TCCCCTAAAC CAGAAAAAGT ATTCAGTTTT CTAAGTCAGG ATGAAGTTGA GAAAATGAAA 
CAAGAAGAAG AAAAGAAGAT TGCTACTCTA ACGCGGGATG AATTAAAGTC GTGGGCACAA 
CTTGATGAAA ATGAAGGTTT GGAAGATTTA GAAGAAACAT TAGAAGATCA GGATAATCGG 
CGACCTAATA GCCGACTGAG TTCGCGAACT TCGATCCCTC TTCTGCAAAA TCTTCCAAGC 
AGTGTAAGGA CAGCAAAAGC AGAACGCCGC TTGAAAGAAA GATTAATACA GGAGGGTATA 
ATTTCGGATG AAGATGAAGA AAGTCATTTA AGTCCTGCCG AACAGAGAGC GTTAAGAGCA 
GAAAAACGTG CGGCATGGAG ACAAGCACGT TTGAAATCTC TGGAACAAGA TGCTCTTCAA 
GCACAGATAG TAATTAAAAA AATGAGTGAA ATGATGGACA CGAATAAAAC AAGTTCAATG 
CAGGATTCTA CAGATATTCC TATTACTCCT GTTTCTGAAT CGGAAAAGAC AACTGATTTC 
ACTACGTTAC GGCCGTCTAG TGCAGATTTT CCCAAACTTG CTGTACGTAG CAAAGTGGGT 
CCCCCTAAAG AGATACGCGA ATCCGAGAAA GTAGTGGACG AGAAGGTCAC TCGACGTACT 
GAGGAATATG TGGATGAGGT GACGGGTGAA CGACGTGTGC GAACAGTCGA ATACGTCGAG 
AAACTCATTG AACGACAGGT TGAAACTCTA CGAGAAAAGA TAATATCATT GGAATTAAGT 
AATGCAGAAG ATGAAATAGA AAGTATTACC GGTACAGGAG CAAGTGATGC TGAAAGCGAA 
AGCGAAGAAA TTACTGTACA ACCTCCTACT ATGACTACTT TACAAGAAAG AAATGAAACA 
ATTATATCTA CAAATGAAGG TGCCGCACCT AAAATAAGTG CAAATACTGT TCTTGTTTCA 
AAAAAAAAGA AACGAAAACG TTCGAAGAAA GGTCGTCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016921377.1
CDS	1476..7637
Translation	MFRCIPIFKGCNRQVEYIDKRHCSLPCVPDDILRYSRSLEELLLDANHIRDLPKNFFRLQKLRKLGLSDNEIHRLPPDIQNFENLVELDVSRNDIPDIPENIKNLRALQVADFSSNPIPRLPAGFVQLRNLTVLGLNDMSLTNLPPDFGSLEALQSLELRENLLKSLPESLSQLYKLERLDLGDNDIEVLPAHIGELPALQELWLDHNQLQHLPPEIGELKTLVCLDVSENRLEDLPEEIGGLEALTDLHLSQNVIEKLPDGLGELKKLTILKVDQNRLSTLNPNIGRCENLQELILTENFLLELPVSIGKLLNLNNLNVDRNSLQSLPTETGNLKQLGVLSLRDNKLQYLPIEVGQCTALHVLDVSGNRLQYLPYSLINLNLKAVWLSKNQAQPMLTFQTDVDEETGQEVLTCFLLPQLEIHPDDSGRLGMLAGMRNAVQDGEIRELGSSDDEGWQEKEASRTHSVKFTDEPPETDKETPFVRQNTPHPKELKAKAHKLFSKGKNDSRSASTDEQDVSQTFGLDKTETDVSTKPNDLTVDLIEQESLKHESVENEQKEIVPGVTDNIDVQSSNEPEIVSNQYIVESNTDVRTEGSISELKDRNDKEDDESENEETQRHVEFSIAEGTDYDGSGDSNRPNRLHRRDTPHHLKNKRIHTAVDKEKVASIIAQALMKKNDEISTSTSAPAETTENFDAQSQDAISDAEPTIEVREEQYEIHIERTTGGLGLSIAGGIGSTPFKGDDEGIFISRVTEGGPADLAGLKVEDKVLSVNGVSVVNVGHYDAVEVLKACGRVLVLVVQREVTRIVPPFEQISSRKNSVCSSLSTSRAPSATSHVSSIGFSHNLENGDALSHDAIKCKKIPEPISSTKVGNEPMVSVLVHTTLIRDQNGLGFSIAGGEGSPPFKDNSDAIYISRITDGGVAQKDGKLLVGDKVISINGVEMRGAKHEQAVALLTGLERFVRLVVEREIPFSQANAATVVTPSEKSPRVIGAPKPYTGLYNANSYIANKPGYTGYRRSIDADRTLSPSPTSKTPPPMKIETTELPKMNGVTESGNIKNVSSISPSPTNSQLHPQPAPRHSISQPTITPTTTTSSTPTSSTEPRSTSPQEDIQVPKPITNEEFQAMIPAHFLRPPTSSPSPDSHQGPIVTVTIKQPDNLPGDVNFPPAPTTLGKVTETITKSTLTETVVTRVTENQLVPPVIIEDVILVKEGSLGFSIIGGTDHSCTPFGAKEPGIFISHVVPGGIAAKSGKLRMGDRILKVNGTDVTKATHQEAVMELLRPGDQIVLTVQHDPLPENYQLVEIEYIPVTELVITKEPGEKLGMHIKGGLRGQKGNPLDHTDEGVFISKINSGGAAKRDGRLKVGMRLLEVNGTSLLGATHQEAVNILRCSGNTITLVVCKGYDKSEIEPVLPISDGRDSKESRTSKELKDATTEGTKSLSQSISSLDRDDEEAATLRQEQEMKAELVAWEQEERERALLEHREKSTPEKVLDVVRAAESLVSKSNSPVDMVVPPKSPGGTKDLKTTTIVMSKHTLAPQNTNSLRKERTGTSVDNPSPKSPISTLTPSMNFDHTTAVQSLPSPKKKSSIPKRSITFADLPPISKEPINYSISAKEDPSPLHDSKFTFDPQTISYKKVYQNPIQATHSRFKLPPTPLTAPKSIEDYGTSISFGKRQNARSVDGNSQVELSPTTSPTPPLVKMSVSDKKKLFESAMEEHLKPSPKPEKVFSFLSQDEVEKMKQEEEKKIATLTRDELKSWAQLDENEGLEDLEETLEDQDNRRPNSRLSSRTSIPLLQNLPSSVRTAKAERRLKERLIQEGIISDEDEESHLSPAEQRALRAEKRAAWRQARLKSLEQDALQAQIVIKKMSEMMDTNKTSSMQDSTDIPITPVSESEKTTDFTTLRPSSADFPKLAVRSKVGPPKEIRESEKVVDEKVTRRTEEYVDEVTGERRVRTVEYVEKLIERQVETLREKIISLELSNAEDEIESITGTGASDAESESEEITVQPPTMTTLQERNETIISTNEGAAPKISANTVLVSKKKKRKRSKKGRH

Target ORF information:

RefSeq Version	XM_017065888.2
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana protein lap4 (LOC108003564), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017065888.2

ORF Insert Sequence:

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGA TATCATCGAG AAAAAACTCA 
GTGTGCTCTA GTTTAAGTAC TAGTAGAGCT CCAAGTGCAA CATCTCATGT TTCATCTATA 
GGTTTTTCAC ATAATTTAGA AAATGGTGAT GCTTTATCTC ATGATGCTAT TAAGTGTAAG 
AAAATTCCTG AACCTATATC TTCAACAAAA GTAGGCAATG AACCAATGGT ATCAGTTCTT 
GTTCACACAA CATTAATACG GGACCAAAAT GGGCTTGGAT TTAGTATTGC TGGTGGAGAA 
GGTTCTCCAC CATTCAAAGA CAATAGTGAT GCGATATATA TTTCAAGAAT AACAGATGGC 
GGCGTAGCAC AAAAAGATGG TAAATTATTA GTTGGAGACA AAGTAATATC TATTAATGGA 
GTTGAGATGA GAGGAGCTAA GCATGAACAA GCAGTTGCTT TATTAACAGG TTTAGAAAGA 
TTTGTTCGAT TAGTAGTTGA ACGCGAAATA CCATTTTCGC AAGCAAATGC AGCTACGGTC 
GTAACACCTT CAGAAAAATC ACCACGAGTG ATCGGTGCAC CTAAACCATA TACAGGATTA 
TATAATGCCA ATAGTTATAT AGCAAATAAA CCGGGATATA CTGGATACAG ACGTTCTATA 
GATGCTGATA GAACATTATC TCCAAGCCCT ACTTCGAAAA CGCCTCCACC TATGAAAATT 
GAAACTACTG AATTACCGAA AATGAACGGT GTTACAGAAT CAGGAAATAT TAAAAATGTT 
TCTTCGATAT CACCAAGTCC AACAAATAGC CAACTACATC CACAGCCTGC TCCTAGGCAC 
AGCATTTCGC AACCAACAAT TACACCTACA ACTACTACGA GTTCAACGCC CACATCTTCT 
ACAGAACCTA GGTCAACCTC ACCCCAGGAA GATATACAGG TACCCAAGCC TATCACCAAT 
GAAGAATTTC AGGCTATGAT TCCTGCTCAT TTCCTTCGTC CTCCTACTTC CTCACCATCG 
CCAGATTCCC ATCAAGGCCC TATCGTGACA GTGACAATAA AACAGCCTGA TAATCTACCT 
GGAGATGTTA ATTTTCCTCC GGCTCCTACA ACACTTGGTA AAGTTACTGA GACCATCACA 
AAGAGCACTC TCACCGAGAC CGTCGTAACT AGGGTGACTG AAAATCAATT GGTACCACCA 
GTAATTATTG AGGACGTGAT TCTTGTAAAA GAGGGATCTC TAGGATTTAG TATTATTGGT 
GGTACGGATC ATTCATGCAC TCCATTTGGT GCAAAGGAAC CTGGAATATT TATATCTCAT 
GTCGTGCCTG GTGGTATAGC TGCAAAATCT GGTAAATTAA GAATGGGTGA TAGAATACTA 
AAGGTAAATG GTACTGATGT AACAAAAGCT ACTCATCAAG AGGCTGTGAT GGAACTTTTG 
CGACCAGGAG ATCAAATAGT GCTTACTGTC CAACACGATC CACTACCCGA AAATTATCAG 
CTGGTCGAAA TAGAGTACAT CCCTGTGACA GAATTAGTTA TTACAAAAGA ACCAGGAGAG 
AAACTGGGCA TGCATATTAA AGGTGGACTT CGAGGACAAA AAGGTAATCC ACTTGATCAT 
ACAGATGAAG GGGTTTTTAT ATCCAAAATC AACTCAGGTG GTGCAGCTAA AAGAGATGGA 
AGACTGAAAG TTGGAATGAG ACTATTAGAA GTTAATGGTA CATCATTATT AGGCGCAACT 
CACCAAGAAG CAGTAAATAT ATTGCGATGT TCAGGAAATA CGATTACTTT GGTGGTTTGT 
AAAGGATATG ATAAGAGTGA AATTGAACCA GTATTACCAA TATCCGATGG AAGAGATTCT 
AAGGAATCTA GAACATCCAA AGAATTAAAG GATGCTACAA CAGAAGGTAC TAAATCGTTA 
TCACAAAGTA TATCCAGTCT GGATCGCGAT GATGAAGAAG CAGCAACTCT TAGACAAGAA 
CAGGAAATGA AAGCTGAACT TGTCGCATGG GAACAAGAAG AAAGAGAACG TGCTTTGCTT 
GAGCATAGAG AAAAATCTAC TCCTGAAAAA GTATTAGATG TAGTAAGAGC AGCTGAATCA 
TTAGTAAGCA AATCAAATAG TCCGGTTGAT ATGGTCGTAC CACCAAAGTC ACCAGGTGGT 
ACGAAAGATC TCAAAACAAC CACTATTGTG ATGAGCAAAC ATACTCTAGC ACCTCAAAAT 
ACAAATTCAC TGAGGAAAGA GAGAACTGGA ACATCAGTGG ACAATCCATC GCCCAAGTCT 
CCGATCTCTA CTTTAACTCC ATCGATGAAT TTCGATCACA CTACTGCTGT CCAAAGCTTG 
CCCTCTCCAA AGAAAAAAAG TTCCATACCA AAACGTAGTA TTACATTTGC TGATCTTCCT 
CCCATTTCTA AAGAACCAAT TAATTATTCC ATTTCGGCGA AAGAAGATCC ATCACCTTTG 
CATGATAGTA AATTTACTTT CGATCCTCAA ACTATTTCTT ATAAGAAAGT CTATCAAAAT 
CCTATCCAAG CTACTCATTC TCGTTTCAAA CTTCCTCCTA CACCTTTAAC TGCTCCTAAA 
TCTATAGAAG ATTATGGTAC TTCAATTTCT TTTGGTAAAC GACAGAACGC ACGCTCTGTT 
GATGGGAATA GTCAGGTTGA GCTTTCTCCA ACAACATCAC CAACACCACC TCTAGTAAAG 
ATGTCGGTTA GTGATAAAAA AAAATTGTTT GAAAGTGCCA TGGAGGAACA TTTAAAACCT 
TCCCCTAAAC CAGAAAAAGT ATTCAGTTTT CTAAGTCAGG ATGAAGTTGA GAAAATGAAA 
CAAGAAGAAG AAAAGAAGAT TGCTACTCTA ACGCGGGATG AATTAAAGTC GTGGGCACAA 
CTTGATGAAA ATGAAGGTTT GGAAGATTTA GAAGAAACAT TAGAAGATCA GGATAATCGG 
CGACCTAATA GCCGACTGAG TTCGCGAACT TCGATCCCTC TTCTGCAAAA TCTTCCAAGC 
AGTGTAAGGA CAGCAAAAGC AGAACGCCGC TTGAAAGAAA GATTAATACA GGAGGGTATA 
ATTTCGGATG AAGATGAAGA AAGTCATTTA AGTCCTGCCG AACAGAGAGC GTTAAGAGCA 
GAAAAACGTG CGGCATGGAG ACAAGCACGT TTGAAATCTC TGGAACAAGA TGCTCTTCAA 
GCACAGATAG TAATTAAAAA AATGAGTGAA ATGATGGACA CGAATAAAAC AAGTTCAATG 
CAGGATTCTA CAGATATTCC TATTACTCCT GTTTCTGAAT CGGAAAAGAC AACTGATTTC 
ACTACGTTAC GGCCGTCTAG TGCAGATTTT CCCAAACTTG CTGTACGTAG CAAAGTGGGT 
CCCCCTAAAG AGATACGCGA ATCCGAGAAA GTAGTGGACG AGAAGGTCAC TCGACGTACT 
GAGGAATATG TGGATGAGGT GACGGGTGAA CGACGTGTGC GAACAGTCGA ATACGTCGAG 
AAACTCATTG AACGACAGGT TGAAACTCTA CGAGAAAAGA TAATATCATT GGAATTAAGT 
AATGCAGAAG ATGAAATAGA AAGTATTACC GGTACAGGAG CAAGTGATGC TGAAAGCGAA 
AGCGAAGAAA TTACTGTACA ACCTCCTACT ATGACTACTT TACAAGAAAG AAATGAAACA 
ATTATATCTA CAAATGAAGG TGCCGCACCT AAAATAAGTG CAAATACTGT TCTTGTTTCA 
AAAAAAAAGA AACGAAAACG TTCGAAGAAA GGTCGTCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108003564 ( XM_017065889.2 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_016921378.1 Apis cerana protein lap4 (LOC108003564), transcript variant X3, mRNA.

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CloneID	OAp29723
Clone ID Related Accession (Same CDS sequence)	XM_017065889.2 , XM_017065889.1
Accession Version	XM_017065889.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-16
Organism	Apis cerana(Asiatic honeybee)
Product	protein lap4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016017567.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 17, 2019 this sequence version replaced XM_017065889.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis cerana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCTGAGCCGA CAATAGAAGT ACGAGAAGAA CAATACGAAA TTCACATCGA AAGAACAACA 
GGTGGTCTAG GCTTATCAAT AGCTGGTGGT ATTGGTTCAA CCCCTTTTAA AGGCGATGAT 
GAAGGCATTT TTATTTCCAG AGTTACTGAA GGTGGACCTG CAGATTTAGC AGGTTTAAAA 
GTGGAAGATA AAGTATTATC TGTTAATGGT GTATCAGTAG TAAATGTAGG TCATTATGAT 
GCTGTAGAAG TTTTGAAAGC TTGTGGACGA GTTCTTGTAT TAGTAGTTCA AAGAGAAGTT 
ACAAGGATAG TGCCTCCATT TGAACAGCAG ATATCATCGA GAAAAAACTC AGTGTGCTCT 
AGTTTAAGTA CTAGTAGAGC TCCAAGTGCA ACATCTCATG TTTCATCTAT AGGTTTTTCA 
CATAATTTAG AAAATGGTGA TGCTTTATCT CATGATGCTA TTAAGTGTAA GAAAATTCCT 
GAACCTATAT CTTCAACAAA AGTAGGCAAT GAACCAATGG TATCAGTTCT TGTTCACACA 
ACATTAATAC GGGACCAAAA TGGGCTTGGA TTTAGTATTG CTGGTGGAGA AGGTTCTCCA 
CCATTCAAAG ACAATAGTGA TGCGATATAT ATTTCAAGAA TAACAGATGG CGGCGTAGCA 
CAAAAAGATG GTAAATTATT AGTTGGAGAC AAAGTAATAT CTATTAATGG AGTTGAGATG 
AGAGGAGCTA AGCATGAACA AGCAGTTGCT TTATTAACAG GTTTAGAAAG ATTTGTTCGA 
TTAGTAGTTG AACGCGAAAT ACCATTTTCG CAAGCAAATG CAGCTACGGT CGTAACACCT 
TCAGAAAAAT CACCACGAGT GATCGGTGCA CCTAAACCAT ATACAGGATT ATATAATGCC 
AATAGTTATA TAGCAAATAA ACCGGGATAT ACTGGATACA GACGTTCTAT AGATGCTGAT 
AGAACATTAT CTCCAAGCCC TACTTCGAAA ACGCCTCCAC CTATGAAAAT TGAAACTACT 
GAATTACCGA AAATGAACGG TGTTACAGAA TCAGGAAATA TTAAAAATGT TTCTTCGATA 
TCACCAAGTC CAACAAATAG CCAACTACAT CCACAGCCTG CTCCTAGGCA CAGCATTTCG 
CAACCAACAA TTACACCTAC AACTACTACG AGTTCAACGC CCACATCTTC TACAGAACCT 
AGGTCAACCT CACCCCAGGA AGATATACAG GTACCCAAGC CTATCACCAA TGAAGAATTT 
CAGGCTATGA TTCCTGCTCA TTTCCTTCGT CCTCCTACTT CCTCACCATC GCCAGATTCC 
CATCAAGGCC CTATCGTGAC AGTGACAATA AAACAGCCTG ATAATCTACC TGGAGATGTT 
AATTTTCCTC CGGCTCCTAC AACACTTGGT AAAGTTACTG AGACCATCAC AAAGAGCACT 
CTCACCGAGA CCGTCGTAAC TAGGGTGACT GAAAATCAAT TGGTACCACC AGTAATTATT 
GAGGACGTGA TTCTTGTAAA AGAGGGATCT CTAGGATTTA GTATTATTGG TGGTACGGAT 
CATTCATGCA CTCCATTTGG TGCAAAGGAA CCTGGAATAT TTATATCTCA TGTCGTGCCT 
GGTGGTATAG CTGCAAAATC TGGTAAATTA AGAATGGGTG ATAGAATACT AAAGGTAAAT 
GGTACTGATG TAACAAAAGC TACTCATCAA GAGGCTGTGA TGGAACTTTT GCGACCAGGA 
GATCAAATAG TGCTTACTGT CCAACACGAT CCACTACCCG AAAATTATCA GCTGGTCGAA 
ATAGAGTACA TCCCTGTGAC AGAATTAGTT ATTACAAAAG AACCAGGAGA GAAACTGGGC 
ATGCATATTA AAGGTGGACT TCGAGGACAA AAAGGTAATC CACTTGATCA TACAGATGAA 
GGGGTTTTTA TATCCAAAAT CAACTCAGGT GGTGCAGCTA AAAGAGATGG AAGACTGAAA 
GTTGGAATGA GACTATTAGA AGTTAATGGT ACATCATTAT TAGGCGCAAC TCACCAAGAA 
GCAGTAAATA TATTGCGATG TTCAGGAAAT ACGATTACTT TGGTGGTTTG TAAAGGATAT 
GATAAGAGTG AAATTGAACC AGTATTACCA ATATCCGATG GAAGAGATTC TAAGGAATCT 
AGAACATCCA AAGAATTAAA GGATGCTACA ACAGAAGGTA CTAAATCGTT ATCACAAAGT 
ATATCCAGTC TGGATCGCGA TGATGAAGAA GCAGCAACTC TTAGACAAGA ACAGGAAATG 
AAAGCTGAAC TTGTCGCATG GGAACAAGAA GAAAGAGAAC GTGCTTTGCT TGAGCATAGA 
GAAAAATCTA CTCCTGAAAA AGTATTAGAT GTAGTAAGAG CAGCTGAATC ATTAGTAAGC 
AAATCAAATA GTCCGGTTGA TATGGTCGTA CCACCAAAGT CACCAGGTGG TACGAAAGAT 
CTCAAAACAA CCACTATTGT GATGAGCAAA CATACTCTAG CACCTCAAAA TACAAATTCA 
CTGAGGAAAG AGAGAACTGG AACATCAGTG GACAATCCAT CGCCCAAGTC TCCGATCTCT 
ACTTTAACTC CATCGATGAA TTTCGATCAC ACTACTGCTG TCCAAAGCTT GCCCTCTCCA 
AAGAAAAAAA GTTCCATACC AAAACGTAGT ATTACATTTG CTGATCTTCC TCCCATTTCT 
AAAGAACCAA TTAATTATTC CATTTCGGCG AAAGAAGATC CATCACCTTT GCATGATAGT 
AAATTTACTT TCGATCCTCA AACTATTTCT TATAAGAAAG TCTATCAAAA TCCTATCCAA 
GCTACTCATT CTCGTTTCAA ACTTCCTCCT ACACCTTTAA CTGCTCCTAA ATCTATAGAA 
GATTATGGTA CTTCAATTTC TTTTGGTAAA CGACAGAACG CACGCTCTGT TGATGGGAAT 
AGTCAGGTTG AGCTTTCTCC AACAACATCA CCAACACCAC CTCTAGTAAA GATGTCGGTT 
AGTGATAAAA AAAAATTGTT TGAAAGTGCC ATGGAGGAAC ATTTAAAACC TTCCCCTAAA 
CCAGAAAAAG TATTCAGTTT TCTAAGTCAG GATGAAGTTG AGAAAATGAA ACAAGAAGAA 
GAAAAGAAGA TTGCTACTCT AACGCGGGAT GAATTAAAGT CGTGGGCACA ACTTGATGAA 
AATGAAGGTT TGGAAGATTT AGAAGAAACA TTAGAAGATC AGGATAATCG GCGACCTAAT 
AGCCGACTGA GTTCGCGAAC TTCGATCCCT CTTCTGCAAA ATCTTCCAAG CAGTGTAAGG 
ACAGCAAAAG CAGAACGCCG CTTGAAAGAA AGATTAATAC AGGAGGGTAT AATTTCGGAT 
GAAGATGAAG AAAGTCATTT AAGTCCTGCC GAACAGAGAG CGTTAAGAGC AGAAAAACGT 
GCGGCATGGA GACAAGCACG TTTGAAATCT CTGGAACAAG ATGCTCTTCA AGCACAGATA 
GTAATTAAAA AAATGAGTGA AATGATGGAC ACGAATAAAA CAAGTTCAAT GCAGGATTCT 
ACAGATATTC CTATTACTCC TGTTTCTGAA TCGGAAAAGA CAACTGATTT CACTACGTTA 
CGGCCGTCTA GTGCAGATTT TCCCAAACTT GCTGTACGTA GCAAAGTGGG TCCCCCTAAA 
GAGATACGCG AATCCGAGAA AGTAGTGGAC GAGAAGGTCA CTCGACGTAC TGAGGAATAT 
GTGGATGAGG TGACGGGTGA ACGACGTGTG CGAACAGTCG AATACGTCGA GAAACTCATT 
GAACGACAGG TTGAAACTCT ACGAGAAAAG ATAATATCAT TGGAATTAAG TAATGCAGAA 
GATGAAATAG AAAGTATTAC CGGTACAGGA GCAAGTGATG CTGAAAGCGA AAGCGAAGAA 
ATTACTGTAC AACCTCCTAC TATGACTACT TTACAAGAAA GAAATGAAAC AATTATATCT 
ACAAATGAAG GTGCCGCACC TAAAATAAGT GCAAATACTG TTCTTGTTTC AAAAAAAAAG 
AAACGAAAAC GTTCGAAGAA AGGTCGTCAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016921378.1
CDS	1476..7628
Translation	MFRCIPIFKGCNRQVEYIDKRHCSLPCVPDDILRYSRSLEELLLDANHIRDLPKNFFRLQKLRKLGLSDNEIHRLPPDIQNFENLVELDVSRNDIPDIPENIKNLRALQVADFSSNPIPRLPAGFVQLRNLTVLGLNDMSLTNLPPDFGSLEALQSLELRENLLKSLPESLSQLYKLERLDLGDNDIEVLPAHIGELPALQELWLDHNQLQHLPPEIGELKTLVCLDVSENRLEDLPEEIGGLEALTDLHLSQNVIEKLPDGLGELKKLTILKVDQNRLSTLNPNIGRCENLQELILTENFLLELPVSIGKLLNLNNLNVDRNSLQSLPTETGNLKQLGVLSLRDNKLQYLPIEVGQCTALHVLDVSGNRLQYLPYSLINLNLKAVWLSKNQAQPMLTFQTDVDEETGQEVLTCFLLPQLEIHPDDSGRLGMLAGMRNAVQDGEIRELGSSDDEGWQEKEASRTHSVKFTDEPPETDKETPFVRQNTPHPKELKAKAHKLFSKGKNDSRSASTDEQDVSQTFGLDKTETDVSTKPNDLTVDLIEQESLKHESVENEQKEIVPGVTDNIDVQSSNEPEIVSNQYIVESNTDVRTEGSISELKDRNDKEDDESENEETQRHVEFSIAEGTDYDGSGDSNRPNRLHRRDTPHHLKNKRIHTAVDKEKVASIIAQALMKKNDEISTSTSAPAETTENFDAQSQDAEPTIEVREEQYEIHIERTTGGLGLSIAGGIGSTPFKGDDEGIFISRVTEGGPADLAGLKVEDKVLSVNGVSVVNVGHYDAVEVLKACGRVLVLVVQREVTRIVPPFEQQISSRKNSVCSSLSTSRAPSATSHVSSIGFSHNLENGDALSHDAIKCKKIPEPISSTKVGNEPMVSVLVHTTLIRDQNGLGFSIAGGEGSPPFKDNSDAIYISRITDGGVAQKDGKLLVGDKVISINGVEMRGAKHEQAVALLTGLERFVRLVVEREIPFSQANAATVVTPSEKSPRVIGAPKPYTGLYNANSYIANKPGYTGYRRSIDADRTLSPSPTSKTPPPMKIETTELPKMNGVTESGNIKNVSSISPSPTNSQLHPQPAPRHSISQPTITPTTTTSSTPTSSTEPRSTSPQEDIQVPKPITNEEFQAMIPAHFLRPPTSSPSPDSHQGPIVTVTIKQPDNLPGDVNFPPAPTTLGKVTETITKSTLTETVVTRVTENQLVPPVIIEDVILVKEGSLGFSIIGGTDHSCTPFGAKEPGIFISHVVPGGIAAKSGKLRMGDRILKVNGTDVTKATHQEAVMELLRPGDQIVLTVQHDPLPENYQLVEIEYIPVTELVITKEPGEKLGMHIKGGLRGQKGNPLDHTDEGVFISKINSGGAAKRDGRLKVGMRLLEVNGTSLLGATHQEAVNILRCSGNTITLVVCKGYDKSEIEPVLPISDGRDSKESRTSKELKDATTEGTKSLSQSISSLDRDDEEAATLRQEQEMKAELVAWEQEERERALLEHREKSTPEKVLDVVRAAESLVSKSNSPVDMVVPPKSPGGTKDLKTTTIVMSKHTLAPQNTNSLRKERTGTSVDNPSPKSPISTLTPSMNFDHTTAVQSLPSPKKKSSIPKRSITFADLPPISKEPINYSISAKEDPSPLHDSKFTFDPQTISYKKVYQNPIQATHSRFKLPPTPLTAPKSIEDYGTSISFGKRQNARSVDGNSQVELSPTTSPTPPLVKMSVSDKKKLFESAMEEHLKPSPKPEKVFSFLSQDEVEKMKQEEEKKIATLTRDELKSWAQLDENEGLEDLEETLEDQDNRRPNSRLSSRTSIPLLQNLPSSVRTAKAERRLKERLIQEGIISDEDEESHLSPAEQRALRAEKRAAWRQARLKSLEQDALQAQIVIKKMSEMMDTNKTSSMQDSTDIPITPVSESEKTTDFTTLRPSSADFPKLAVRSKVGPPKEIRESEKVVDEKVTRRTEEYVDEVTGERRVRTVEYVEKLIERQVETLREKIISLELSNAEDEIESITGTGASDAESESEEITVQPPTMTTLQERNETIISTNEGAAPKISANTVLVSKKKKRKRSKKGRH

Target ORF information:

RefSeq Version	XM_017065889.2
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana protein lap4 (LOC108003564), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017065889.2

ORF Insert Sequence:

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCTGAGCCGA CAATAGAAGT ACGAGAAGAA CAATACGAAA TTCACATCGA AAGAACAACA 
GGTGGTCTAG GCTTATCAAT AGCTGGTGGT ATTGGTTCAA CCCCTTTTAA AGGCGATGAT 
GAAGGCATTT TTATTTCCAG AGTTACTGAA GGTGGACCTG CAGATTTAGC AGGTTTAAAA 
GTGGAAGATA AAGTATTATC TGTTAATGGT GTATCAGTAG TAAATGTAGG TCATTATGAT 
GCTGTAGAAG TTTTGAAAGC TTGTGGACGA GTTCTTGTAT TAGTAGTTCA AAGAGAAGTT 
ACAAGGATAG TGCCTCCATT TGAACAGCAG ATATCATCGA GAAAAAACTC AGTGTGCTCT 
AGTTTAAGTA CTAGTAGAGC TCCAAGTGCA ACATCTCATG TTTCATCTAT AGGTTTTTCA 
CATAATTTAG AAAATGGTGA TGCTTTATCT CATGATGCTA TTAAGTGTAA GAAAATTCCT 
GAACCTATAT CTTCAACAAA AGTAGGCAAT GAACCAATGG TATCAGTTCT TGTTCACACA 
ACATTAATAC GGGACCAAAA TGGGCTTGGA TTTAGTATTG CTGGTGGAGA AGGTTCTCCA 
CCATTCAAAG ACAATAGTGA TGCGATATAT ATTTCAAGAA TAACAGATGG CGGCGTAGCA 
CAAAAAGATG GTAAATTATT AGTTGGAGAC AAAGTAATAT CTATTAATGG AGTTGAGATG 
AGAGGAGCTA AGCATGAACA AGCAGTTGCT TTATTAACAG GTTTAGAAAG ATTTGTTCGA 
TTAGTAGTTG AACGCGAAAT ACCATTTTCG CAAGCAAATG CAGCTACGGT CGTAACACCT 
TCAGAAAAAT CACCACGAGT GATCGGTGCA CCTAAACCAT ATACAGGATT ATATAATGCC 
AATAGTTATA TAGCAAATAA ACCGGGATAT ACTGGATACA GACGTTCTAT AGATGCTGAT 
AGAACATTAT CTCCAAGCCC TACTTCGAAA ACGCCTCCAC CTATGAAAAT TGAAACTACT 
GAATTACCGA AAATGAACGG TGTTACAGAA TCAGGAAATA TTAAAAATGT TTCTTCGATA 
TCACCAAGTC CAACAAATAG CCAACTACAT CCACAGCCTG CTCCTAGGCA CAGCATTTCG 
CAACCAACAA TTACACCTAC AACTACTACG AGTTCAACGC CCACATCTTC TACAGAACCT 
AGGTCAACCT CACCCCAGGA AGATATACAG GTACCCAAGC CTATCACCAA TGAAGAATTT 
CAGGCTATGA TTCCTGCTCA TTTCCTTCGT CCTCCTACTT CCTCACCATC GCCAGATTCC 
CATCAAGGCC CTATCGTGAC AGTGACAATA AAACAGCCTG ATAATCTACC TGGAGATGTT 
AATTTTCCTC CGGCTCCTAC AACACTTGGT AAAGTTACTG AGACCATCAC AAAGAGCACT 
CTCACCGAGA CCGTCGTAAC TAGGGTGACT GAAAATCAAT TGGTACCACC AGTAATTATT 
GAGGACGTGA TTCTTGTAAA AGAGGGATCT CTAGGATTTA GTATTATTGG TGGTACGGAT 
CATTCATGCA CTCCATTTGG TGCAAAGGAA CCTGGAATAT TTATATCTCA TGTCGTGCCT 
GGTGGTATAG CTGCAAAATC TGGTAAATTA AGAATGGGTG ATAGAATACT AAAGGTAAAT 
GGTACTGATG TAACAAAAGC TACTCATCAA GAGGCTGTGA TGGAACTTTT GCGACCAGGA 
GATCAAATAG TGCTTACTGT CCAACACGAT CCACTACCCG AAAATTATCA GCTGGTCGAA 
ATAGAGTACA TCCCTGTGAC AGAATTAGTT ATTACAAAAG AACCAGGAGA GAAACTGGGC 
ATGCATATTA AAGGTGGACT TCGAGGACAA AAAGGTAATC CACTTGATCA TACAGATGAA 
GGGGTTTTTA TATCCAAAAT CAACTCAGGT GGTGCAGCTA AAAGAGATGG AAGACTGAAA 
GTTGGAATGA GACTATTAGA AGTTAATGGT ACATCATTAT TAGGCGCAAC TCACCAAGAA 
GCAGTAAATA TATTGCGATG TTCAGGAAAT ACGATTACTT TGGTGGTTTG TAAAGGATAT 
GATAAGAGTG AAATTGAACC AGTATTACCA ATATCCGATG GAAGAGATTC TAAGGAATCT 
AGAACATCCA AAGAATTAAA GGATGCTACA ACAGAAGGTA CTAAATCGTT ATCACAAAGT 
ATATCCAGTC TGGATCGCGA TGATGAAGAA GCAGCAACTC TTAGACAAGA ACAGGAAATG 
AAAGCTGAAC TTGTCGCATG GGAACAAGAA GAAAGAGAAC GTGCTTTGCT TGAGCATAGA 
GAAAAATCTA CTCCTGAAAA AGTATTAGAT GTAGTAAGAG CAGCTGAATC ATTAGTAAGC 
AAATCAAATA GTCCGGTTGA TATGGTCGTA CCACCAAAGT CACCAGGTGG TACGAAAGAT 
CTCAAAACAA CCACTATTGT GATGAGCAAA CATACTCTAG CACCTCAAAA TACAAATTCA 
CTGAGGAAAG AGAGAACTGG AACATCAGTG GACAATCCAT CGCCCAAGTC TCCGATCTCT 
ACTTTAACTC CATCGATGAA TTTCGATCAC ACTACTGCTG TCCAAAGCTT GCCCTCTCCA 
AAGAAAAAAA GTTCCATACC AAAACGTAGT ATTACATTTG CTGATCTTCC TCCCATTTCT 
AAAGAACCAA TTAATTATTC CATTTCGGCG AAAGAAGATC CATCACCTTT GCATGATAGT 
AAATTTACTT TCGATCCTCA AACTATTTCT TATAAGAAAG TCTATCAAAA TCCTATCCAA 
GCTACTCATT CTCGTTTCAA ACTTCCTCCT ACACCTTTAA CTGCTCCTAA ATCTATAGAA 
GATTATGGTA CTTCAATTTC TTTTGGTAAA CGACAGAACG CACGCTCTGT TGATGGGAAT 
AGTCAGGTTG AGCTTTCTCC AACAACATCA CCAACACCAC CTCTAGTAAA GATGTCGGTT 
AGTGATAAAA AAAAATTGTT TGAAAGTGCC ATGGAGGAAC ATTTAAAACC TTCCCCTAAA 
CCAGAAAAAG TATTCAGTTT TCTAAGTCAG GATGAAGTTG AGAAAATGAA ACAAGAAGAA 
GAAAAGAAGA TTGCTACTCT AACGCGGGAT GAATTAAAGT CGTGGGCACA ACTTGATGAA 
AATGAAGGTT TGGAAGATTT AGAAGAAACA TTAGAAGATC AGGATAATCG GCGACCTAAT 
AGCCGACTGA GTTCGCGAAC TTCGATCCCT CTTCTGCAAA ATCTTCCAAG CAGTGTAAGG 
ACAGCAAAAG CAGAACGCCG CTTGAAAGAA AGATTAATAC AGGAGGGTAT AATTTCGGAT 
GAAGATGAAG AAAGTCATTT AAGTCCTGCC GAACAGAGAG CGTTAAGAGC AGAAAAACGT 
GCGGCATGGA GACAAGCACG TTTGAAATCT CTGGAACAAG ATGCTCTTCA AGCACAGATA 
GTAATTAAAA AAATGAGTGA AATGATGGAC ACGAATAAAA CAAGTTCAAT GCAGGATTCT 
ACAGATATTC CTATTACTCC TGTTTCTGAA TCGGAAAAGA CAACTGATTT CACTACGTTA 
CGGCCGTCTA GTGCAGATTT TCCCAAACTT GCTGTACGTA GCAAAGTGGG TCCCCCTAAA 
GAGATACGCG AATCCGAGAA AGTAGTGGAC GAGAAGGTCA CTCGACGTAC TGAGGAATAT 
GTGGATGAGG TGACGGGTGA ACGACGTGTG CGAACAGTCG AATACGTCGA GAAACTCATT 
GAACGACAGG TTGAAACTCT ACGAGAAAAG ATAATATCAT TGGAATTAAG TAATGCAGAA 
GATGAAATAG AAAGTATTAC CGGTACAGGA GCAAGTGATG CTGAAAGCGA AAGCGAAGAA 
ATTACTGTAC AACCTCCTAC TATGACTACT TTACAAGAAA GAAATGAAAC AATTATATCT 
ACAAATGAAG GTGCCGCACC TAAAATAAGT GCAAATACTG TTCTTGTTTC AAAAAAAAAG 
AAACGAAAAC GTTCGAAGAA AGGTCGTCAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108003564 ( XM_017065889.1 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_016921378.1 Apis cerana protein lap4 (LOC108003564), transcript variant X3, mRNA.

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CloneID	OAp29723
Clone ID Related Accession (Same CDS sequence)	XM_017065889.2 , XM_017065889.1
Accession Version	XM_017065889.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-08
Organism	Apis cerana(Asiatic honeybee)
Product	protein lap4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016017567.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis cerana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCTGAGCCGA CAATAGAAGT ACGAGAAGAA CAATACGAAA TTCACATCGA AAGAACAACA 
GGTGGTCTAG GCTTATCAAT AGCTGGTGGT ATTGGTTCAA CCCCTTTTAA AGGCGATGAT 
GAAGGCATTT TTATTTCCAG AGTTACTGAA GGTGGACCTG CAGATTTAGC AGGTTTAAAA 
GTGGAAGATA AAGTATTATC TGTTAATGGT GTATCAGTAG TAAATGTAGG TCATTATGAT 
GCTGTAGAAG TTTTGAAAGC TTGTGGACGA GTTCTTGTAT TAGTAGTTCA AAGAGAAGTT 
ACAAGGATAG TGCCTCCATT TGAACAGCAG ATATCATCGA GAAAAAACTC AGTGTGCTCT 
AGTTTAAGTA CTAGTAGAGC TCCAAGTGCA ACATCTCATG TTTCATCTAT AGGTTTTTCA 
CATAATTTAG AAAATGGTGA TGCTTTATCT CATGATGCTA TTAAGTGTAA GAAAATTCCT 
GAACCTATAT CTTCAACAAA AGTAGGCAAT GAACCAATGG TATCAGTTCT TGTTCACACA 
ACATTAATAC GGGACCAAAA TGGGCTTGGA TTTAGTATTG CTGGTGGAGA AGGTTCTCCA 
CCATTCAAAG ACAATAGTGA TGCGATATAT ATTTCAAGAA TAACAGATGG CGGCGTAGCA 
CAAAAAGATG GTAAATTATT AGTTGGAGAC AAAGTAATAT CTATTAATGG AGTTGAGATG 
AGAGGAGCTA AGCATGAACA AGCAGTTGCT TTATTAACAG GTTTAGAAAG ATTTGTTCGA 
TTAGTAGTTG AACGCGAAAT ACCATTTTCG CAAGCAAATG CAGCTACGGT CGTAACACCT 
TCAGAAAAAT CACCACGAGT GATCGGTGCA CCTAAACCAT ATACAGGATT ATATAATGCC 
AATAGTTATA TAGCAAATAA ACCGGGATAT ACTGGATACA GACGTTCTAT AGATGCTGAT 
AGAACATTAT CTCCAAGCCC TACTTCGAAA ACGCCTCCAC CTATGAAAAT TGAAACTACT 
GAATTACCGA AAATGAACGG TGTTACAGAA TCAGGAAATA TTAAAAATGT TTCTTCGATA 
TCACCAAGTC CAACAAATAG CCAACTACAT CCACAGCCTG CTCCTAGGCA CAGCATTTCG 
CAACCAACAA TTACACCTAC AACTACTACG AGTTCAACGC CCACATCTTC TACAGAACCT 
AGGTCAACCT CACCCCAGGA AGATATACAG GTACCCAAGC CTATCACCAA TGAAGAATTT 
CAGGCTATGA TTCCTGCTCA TTTCCTTCGT CCTCCTACTT CCTCACCATC GCCAGATTCC 
CATCAAGGCC CTATCGTGAC AGTGACAATA AAACAGCCTG ATAATCTACC TGGAGATGTT 
AATTTTCCTC CGGCTCCTAC AACACTTGGT AAAGTTACTG AGACCATCAC AAAGAGCACT 
CTCACCGAGA CCGTCGTAAC TAGGGTGACT GAAAATCAAT TGGTACCACC AGTAATTATT 
GAGGACGTGA TTCTTGTAAA AGAGGGATCT CTAGGATTTA GTATTATTGG TGGTACGGAT 
CATTCATGCA CTCCATTTGG TGCAAAGGAA CCTGGAATAT TTATATCTCA TGTCGTGCCT 
GGTGGTATAG CTGCAAAATC TGGTAAATTA AGAATGGGTG ATAGAATACT AAAGGTAAAT 
GGTACTGATG TAACAAAAGC TACTCATCAA GAGGCTGTGA TGGAACTTTT GCGACCAGGA 
GATCAAATAG TGCTTACTGT CCAACACGAT CCACTACCCG AAAATTATCA GCTGGTCGAA 
ATAGAGTACA TCCCTGTGAC AGAATTAGTT ATTACAAAAG AACCAGGAGA GAAACTGGGC 
ATGCATATTA AAGGTGGACT TCGAGGACAA AAAGGTAATC CACTTGATCA TACAGATGAA 
GGGGTTTTTA TATCCAAAAT CAACTCAGGT GGTGCAGCTA AAAGAGATGG AAGACTGAAA 
GTTGGAATGA GACTATTAGA AGTTAATGGT ACATCATTAT TAGGCGCAAC TCACCAAGAA 
GCAGTAAATA TATTGCGATG TTCAGGAAAT ACGATTACTT TGGTGGTTTG TAAAGGATAT 
GATAAGAGTG AAATTGAACC AGTATTACCA ATATCCGATG GAAGAGATTC TAAGGAATCT 
AGAACATCCA AAGAATTAAA GGATGCTACA ACAGAAGGTA CTAAATCGTT ATCACAAAGT 
ATATCCAGTC TGGATCGCGA TGATGAAGAA GCAGCAACTC TTAGACAAGA ACAGGAAATG 
AAAGCTGAAC TTGTCGCATG GGAACAAGAA GAAAGAGAAC GTGCTTTGCT TGAGCATAGA 
GAAAAATCTA CTCCTGAAAA AGTATTAGAT GTAGTAAGAG CAGCTGAATC ATTAGTAAGC 
AAATCAAATA GTCCGGTTGA TATGGTCGTA CCACCAAAGT CACCAGGTGG TACGAAAGAT 
CTCAAAACAA CCACTATTGT GATGAGCAAA CATACTCTAG CACCTCAAAA TACAAATTCA 
CTGAGGAAAG AGAGAACTGG AACATCAGTG GACAATCCAT CGCCCAAGTC TCCGATCTCT 
ACTTTAACTC CATCGATGAA TTTCGATCAC ACTACTGCTG TCCAAAGCTT GCCCTCTCCA 
AAGAAAAAAA GTTCCATACC AAAACGTAGT ATTACATTTG CTGATCTTCC TCCCATTTCT 
AAAGAACCAA TTAATTATTC CATTTCGGCG AAAGAAGATC CATCACCTTT GCATGATAGT 
AAATTTACTT TCGATCCTCA AACTATTTCT TATAAGAAAG TCTATCAAAA TCCTATCCAA 
GCTACTCATT CTCGTTTCAA ACTTCCTCCT ACACCTTTAA CTGCTCCTAA ATCTATAGAA 
GATTATGGTA CTTCAATTTC TTTTGGTAAA CGACAGAACG CACGCTCTGT TGATGGGAAT 
AGTCAGGTTG AGCTTTCTCC AACAACATCA CCAACACCAC CTCTAGTAAA GATGTCGGTT 
AGTGATAAAA AAAAATTGTT TGAAAGTGCC ATGGAGGAAC ATTTAAAACC TTCCCCTAAA 
CCAGAAAAAG TATTCAGTTT TCTAAGTCAG GATGAAGTTG AGAAAATGAA ACAAGAAGAA 
GAAAAGAAGA TTGCTACTCT AACGCGGGAT GAATTAAAGT CGTGGGCACA ACTTGATGAA 
AATGAAGGTT TGGAAGATTT AGAAGAAACA TTAGAAGATC AGGATAATCG GCGACCTAAT 
AGCCGACTGA GTTCGCGAAC TTCGATCCCT CTTCTGCAAA ATCTTCCAAG CAGTGTAAGG 
ACAGCAAAAG CAGAACGCCG CTTGAAAGAA AGATTAATAC AGGAGGGTAT AATTTCGGAT 
GAAGATGAAG AAAGTCATTT AAGTCCTGCC GAACAGAGAG CGTTAAGAGC AGAAAAACGT 
GCGGCATGGA GACAAGCACG TTTGAAATCT CTGGAACAAG ATGCTCTTCA AGCACAGATA 
GTAATTAAAA AAATGAGTGA AATGATGGAC ACGAATAAAA CAAGTTCAAT GCAGGATTCT 
ACAGATATTC CTATTACTCC TGTTTCTGAA TCGGAAAAGA CAACTGATTT CACTACGTTA 
CGGCCGTCTA GTGCAGATTT TCCCAAACTT GCTGTACGTA GCAAAGTGGG TCCCCCTAAA 
GAGATACGCG AATCCGAGAA AGTAGTGGAC GAGAAGGTCA CTCGACGTAC TGAGGAATAT 
GTGGATGAGG TGACGGGTGA ACGACGTGTG CGAACAGTCG AATACGTCGA GAAACTCATT 
GAACGACAGG TTGAAACTCT ACGAGAAAAG ATAATATCAT TGGAATTAAG TAATGCAGAA 
GATGAAATAG AAAGTATTAC CGGTACAGGA GCAAGTGATG CTGAAAGCGA AAGCGAAGAA 
ATTACTGTAC AACCTCCTAC TATGACTACT TTACAAGAAA GAAATGAAAC AATTATATCT 
ACAAATGAAG GTGCCGCACC TAAAATAAGT GCAAATACTG TTCTTGTTTC AAAAAAAAAG 
AAACGAAAAC GTTCGAAGAA AGGTCGTCAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016921378.1
CDS	233..6385
Translation	MFRCIPIFKGCNRQVEYIDKRHCSLPCVPDDILRYSRSLEELLLDANHIRDLPKNFFRLQKLRKLGLSDNEIHRLPPDIQNFENLVELDVSRNDIPDIPENIKNLRALQVADFSSNPIPRLPAGFVQLRNLTVLGLNDMSLTNLPPDFGSLEALQSLELRENLLKSLPESLSQLYKLERLDLGDNDIEVLPAHIGELPALQELWLDHNQLQHLPPEIGELKTLVCLDVSENRLEDLPEEIGGLEALTDLHLSQNVIEKLPDGLGELKKLTILKVDQNRLSTLNPNIGRCENLQELILTENFLLELPVSIGKLLNLNNLNVDRNSLQSLPTETGNLKQLGVLSLRDNKLQYLPIEVGQCTALHVLDVSGNRLQYLPYSLINLNLKAVWLSKNQAQPMLTFQTDVDEETGQEVLTCFLLPQLEIHPDDSGRLGMLAGMRNAVQDGEIRELGSSDDEGWQEKEASRTHSVKFTDEPPETDKETPFVRQNTPHPKELKAKAHKLFSKGKNDSRSASTDEQDVSQTFGLDKTETDVSTKPNDLTVDLIEQESLKHESVENEQKEIVPGVTDNIDVQSSNEPEIVSNQYIVESNTDVRTEGSISELKDRNDKEDDESENEETQRHVEFSIAEGTDYDGSGDSNRPNRLHRRDTPHHLKNKRIHTAVDKEKVASIIAQALMKKNDEISTSTSAPAETTENFDAQSQDAEPTIEVREEQYEIHIERTTGGLGLSIAGGIGSTPFKGDDEGIFISRVTEGGPADLAGLKVEDKVLSVNGVSVVNVGHYDAVEVLKACGRVLVLVVQREVTRIVPPFEQQISSRKNSVCSSLSTSRAPSATSHVSSIGFSHNLENGDALSHDAIKCKKIPEPISSTKVGNEPMVSVLVHTTLIRDQNGLGFSIAGGEGSPPFKDNSDAIYISRITDGGVAQKDGKLLVGDKVISINGVEMRGAKHEQAVALLTGLERFVRLVVEREIPFSQANAATVVTPSEKSPRVIGAPKPYTGLYNANSYIANKPGYTGYRRSIDADRTLSPSPTSKTPPPMKIETTELPKMNGVTESGNIKNVSSISPSPTNSQLHPQPAPRHSISQPTITPTTTTSSTPTSSTEPRSTSPQEDIQVPKPITNEEFQAMIPAHFLRPPTSSPSPDSHQGPIVTVTIKQPDNLPGDVNFPPAPTTLGKVTETITKSTLTETVVTRVTENQLVPPVIIEDVILVKEGSLGFSIIGGTDHSCTPFGAKEPGIFISHVVPGGIAAKSGKLRMGDRILKVNGTDVTKATHQEAVMELLRPGDQIVLTVQHDPLPENYQLVEIEYIPVTELVITKEPGEKLGMHIKGGLRGQKGNPLDHTDEGVFISKINSGGAAKRDGRLKVGMRLLEVNGTSLLGATHQEAVNILRCSGNTITLVVCKGYDKSEIEPVLPISDGRDSKESRTSKELKDATTEGTKSLSQSISSLDRDDEEAATLRQEQEMKAELVAWEQEERERALLEHREKSTPEKVLDVVRAAESLVSKSNSPVDMVVPPKSPGGTKDLKTTTIVMSKHTLAPQNTNSLRKERTGTSVDNPSPKSPISTLTPSMNFDHTTAVQSLPSPKKKSSIPKRSITFADLPPISKEPINYSISAKEDPSPLHDSKFTFDPQTISYKKVYQNPIQATHSRFKLPPTPLTAPKSIEDYGTSISFGKRQNARSVDGNSQVELSPTTSPTPPLVKMSVSDKKKLFESAMEEHLKPSPKPEKVFSFLSQDEVEKMKQEEEKKIATLTRDELKSWAQLDENEGLEDLEETLEDQDNRRPNSRLSSRTSIPLLQNLPSSVRTAKAERRLKERLIQEGIISDEDEESHLSPAEQRALRAEKRAAWRQARLKSLEQDALQAQIVIKKMSEMMDTNKTSSMQDSTDIPITPVSESEKTTDFTTLRPSSADFPKLAVRSKVGPPKEIRESEKVVDEKVTRRTEEYVDEVTGERRVRTVEYVEKLIERQVETLREKIISLELSNAEDEIESITGTGASDAESESEEITVQPPTMTTLQERNETIISTNEGAAPKISANTVLVSKKKKRKRSKKGRH

Target ORF information:

RefSeq Version	XM_017065889.1
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana protein lap4 (LOC108003564), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017065889.1

ORF Insert Sequence:

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCTGAGCCGA CAATAGAAGT ACGAGAAGAA CAATACGAAA TTCACATCGA AAGAACAACA 
GGTGGTCTAG GCTTATCAAT AGCTGGTGGT ATTGGTTCAA CCCCTTTTAA AGGCGATGAT 
GAAGGCATTT TTATTTCCAG AGTTACTGAA GGTGGACCTG CAGATTTAGC AGGTTTAAAA 
GTGGAAGATA AAGTATTATC TGTTAATGGT GTATCAGTAG TAAATGTAGG TCATTATGAT 
GCTGTAGAAG TTTTGAAAGC TTGTGGACGA GTTCTTGTAT TAGTAGTTCA AAGAGAAGTT 
ACAAGGATAG TGCCTCCATT TGAACAGCAG ATATCATCGA GAAAAAACTC AGTGTGCTCT 
AGTTTAAGTA CTAGTAGAGC TCCAAGTGCA ACATCTCATG TTTCATCTAT AGGTTTTTCA 
CATAATTTAG AAAATGGTGA TGCTTTATCT CATGATGCTA TTAAGTGTAA GAAAATTCCT 
GAACCTATAT CTTCAACAAA AGTAGGCAAT GAACCAATGG TATCAGTTCT TGTTCACACA 
ACATTAATAC GGGACCAAAA TGGGCTTGGA TTTAGTATTG CTGGTGGAGA AGGTTCTCCA 
CCATTCAAAG ACAATAGTGA TGCGATATAT ATTTCAAGAA TAACAGATGG CGGCGTAGCA 
CAAAAAGATG GTAAATTATT AGTTGGAGAC AAAGTAATAT CTATTAATGG AGTTGAGATG 
AGAGGAGCTA AGCATGAACA AGCAGTTGCT TTATTAACAG GTTTAGAAAG ATTTGTTCGA 
TTAGTAGTTG AACGCGAAAT ACCATTTTCG CAAGCAAATG CAGCTACGGT CGTAACACCT 
TCAGAAAAAT CACCACGAGT GATCGGTGCA CCTAAACCAT ATACAGGATT ATATAATGCC 
AATAGTTATA TAGCAAATAA ACCGGGATAT ACTGGATACA GACGTTCTAT AGATGCTGAT 
AGAACATTAT CTCCAAGCCC TACTTCGAAA ACGCCTCCAC CTATGAAAAT TGAAACTACT 
GAATTACCGA AAATGAACGG TGTTACAGAA TCAGGAAATA TTAAAAATGT TTCTTCGATA 
TCACCAAGTC CAACAAATAG CCAACTACAT CCACAGCCTG CTCCTAGGCA CAGCATTTCG 
CAACCAACAA TTACACCTAC AACTACTACG AGTTCAACGC CCACATCTTC TACAGAACCT 
AGGTCAACCT CACCCCAGGA AGATATACAG GTACCCAAGC CTATCACCAA TGAAGAATTT 
CAGGCTATGA TTCCTGCTCA TTTCCTTCGT CCTCCTACTT CCTCACCATC GCCAGATTCC 
CATCAAGGCC CTATCGTGAC AGTGACAATA AAACAGCCTG ATAATCTACC TGGAGATGTT 
AATTTTCCTC CGGCTCCTAC AACACTTGGT AAAGTTACTG AGACCATCAC AAAGAGCACT 
CTCACCGAGA CCGTCGTAAC TAGGGTGACT GAAAATCAAT TGGTACCACC AGTAATTATT 
GAGGACGTGA TTCTTGTAAA AGAGGGATCT CTAGGATTTA GTATTATTGG TGGTACGGAT 
CATTCATGCA CTCCATTTGG TGCAAAGGAA CCTGGAATAT TTATATCTCA TGTCGTGCCT 
GGTGGTATAG CTGCAAAATC TGGTAAATTA AGAATGGGTG ATAGAATACT AAAGGTAAAT 
GGTACTGATG TAACAAAAGC TACTCATCAA GAGGCTGTGA TGGAACTTTT GCGACCAGGA 
GATCAAATAG TGCTTACTGT CCAACACGAT CCACTACCCG AAAATTATCA GCTGGTCGAA 
ATAGAGTACA TCCCTGTGAC AGAATTAGTT ATTACAAAAG AACCAGGAGA GAAACTGGGC 
ATGCATATTA AAGGTGGACT TCGAGGACAA AAAGGTAATC CACTTGATCA TACAGATGAA 
GGGGTTTTTA TATCCAAAAT CAACTCAGGT GGTGCAGCTA AAAGAGATGG AAGACTGAAA 
GTTGGAATGA GACTATTAGA AGTTAATGGT ACATCATTAT TAGGCGCAAC TCACCAAGAA 
GCAGTAAATA TATTGCGATG TTCAGGAAAT ACGATTACTT TGGTGGTTTG TAAAGGATAT 
GATAAGAGTG AAATTGAACC AGTATTACCA ATATCCGATG GAAGAGATTC TAAGGAATCT 
AGAACATCCA AAGAATTAAA GGATGCTACA ACAGAAGGTA CTAAATCGTT ATCACAAAGT 
ATATCCAGTC TGGATCGCGA TGATGAAGAA GCAGCAACTC TTAGACAAGA ACAGGAAATG 
AAAGCTGAAC TTGTCGCATG GGAACAAGAA GAAAGAGAAC GTGCTTTGCT TGAGCATAGA 
GAAAAATCTA CTCCTGAAAA AGTATTAGAT GTAGTAAGAG CAGCTGAATC ATTAGTAAGC 
AAATCAAATA GTCCGGTTGA TATGGTCGTA CCACCAAAGT CACCAGGTGG TACGAAAGAT 
CTCAAAACAA CCACTATTGT GATGAGCAAA CATACTCTAG CACCTCAAAA TACAAATTCA 
CTGAGGAAAG AGAGAACTGG AACATCAGTG GACAATCCAT CGCCCAAGTC TCCGATCTCT 
ACTTTAACTC CATCGATGAA TTTCGATCAC ACTACTGCTG TCCAAAGCTT GCCCTCTCCA 
AAGAAAAAAA GTTCCATACC AAAACGTAGT ATTACATTTG CTGATCTTCC TCCCATTTCT 
AAAGAACCAA TTAATTATTC CATTTCGGCG AAAGAAGATC CATCACCTTT GCATGATAGT 
AAATTTACTT TCGATCCTCA AACTATTTCT TATAAGAAAG TCTATCAAAA TCCTATCCAA 
GCTACTCATT CTCGTTTCAA ACTTCCTCCT ACACCTTTAA CTGCTCCTAA ATCTATAGAA 
GATTATGGTA CTTCAATTTC TTTTGGTAAA CGACAGAACG CACGCTCTGT TGATGGGAAT 
AGTCAGGTTG AGCTTTCTCC AACAACATCA CCAACACCAC CTCTAGTAAA GATGTCGGTT 
AGTGATAAAA AAAAATTGTT TGAAAGTGCC ATGGAGGAAC ATTTAAAACC TTCCCCTAAA 
CCAGAAAAAG TATTCAGTTT TCTAAGTCAG GATGAAGTTG AGAAAATGAA ACAAGAAGAA 
GAAAAGAAGA TTGCTACTCT AACGCGGGAT GAATTAAAGT CGTGGGCACA ACTTGATGAA 
AATGAAGGTT TGGAAGATTT AGAAGAAACA TTAGAAGATC AGGATAATCG GCGACCTAAT 
AGCCGACTGA GTTCGCGAAC TTCGATCCCT CTTCTGCAAA ATCTTCCAAG CAGTGTAAGG 
ACAGCAAAAG CAGAACGCCG CTTGAAAGAA AGATTAATAC AGGAGGGTAT AATTTCGGAT 
GAAGATGAAG AAAGTCATTT AAGTCCTGCC GAACAGAGAG CGTTAAGAGC AGAAAAACGT 
GCGGCATGGA GACAAGCACG TTTGAAATCT CTGGAACAAG ATGCTCTTCA AGCACAGATA 
GTAATTAAAA AAATGAGTGA AATGATGGAC ACGAATAAAA CAAGTTCAAT GCAGGATTCT 
ACAGATATTC CTATTACTCC TGTTTCTGAA TCGGAAAAGA CAACTGATTT CACTACGTTA 
CGGCCGTCTA GTGCAGATTT TCCCAAACTT GCTGTACGTA GCAAAGTGGG TCCCCCTAAA 
GAGATACGCG AATCCGAGAA AGTAGTGGAC GAGAAGGTCA CTCGACGTAC TGAGGAATAT 
GTGGATGAGG TGACGGGTGA ACGACGTGTG CGAACAGTCG AATACGTCGA GAAACTCATT 
GAACGACAGG TTGAAACTCT ACGAGAAAAG ATAATATCAT TGGAATTAAG TAATGCAGAA 
GATGAAATAG AAAGTATTAC CGGTACAGGA GCAAGTGATG CTGAAAGCGA AAGCGAAGAA 
ATTACTGTAC AACCTCCTAC TATGACTACT TTACAAGAAA GAAATGAAAC AATTATATCT 
ACAAATGAAG GTGCCGCACC TAAAATAAGT GCAAATACTG TTCTTGTTTC AAAAAAAAAG 
AAACGAAAAC GTTCGAAGAA AGGTCGTCAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108003564 ( XM_017065890.2 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_016921379.1 Apis cerana protein lap4 (LOC108003564), transcript variant X4, mRNA.

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CloneID	OAp29724
Clone ID Related Accession (Same CDS sequence)	XM_017065890.2 , XM_017065890.1
Accession Version	XM_017065890.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-16
Organism	Apis cerana(Asiatic honeybee)
Product	protein lap4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016017567.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 17, 2019 this sequence version replaced XM_017065890.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis cerana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCTGAGCCGA CAATAGAAGT ACGAGAAGAA CAATACGAAA TTCACATCGA AAGAACAACA 
GGTGGTCTAG GCTTATCAAT AGCTGGTGGT ATTGGTTCAA CCCCTTTTAA AGGCGATGAT 
GAAGGCATTT TTATTTCCAG AGTTACTGAA GGTGGACCTG CAGATTTAGC AGGTTTAAAA 
GTGGAAGATA AAGTATTATC TGTTAATGGT GTATCAGTAG TAAATGTAGG TCATTATGAT 
GCTGTAGAAG TTTTGAAAGC TTGTGGACGA GTTCTTGTAT TAGTAGTTCA AAGAGAAGTT 
ACAAGGATAG TGCCTCCATT TGAACAGATA TCATCGAGAA AAAACTCAGT GTGCTCTAGT 
TTAAGTACTA GTAGAGCTCC AAGTGCAACA TCTCATGTTT CATCTATAGG TTTTTCACAT 
AATTTAGAAA ATGGTGATGC TTTATCTCAT GATGCTATTA AGTGTAAGAA AATTCCTGAA 
CCTATATCTT CAACAAAAGT AGGCAATGAA CCAATGGTAT CAGTTCTTGT TCACACAACA 
TTAATACGGG ACCAAAATGG GCTTGGATTT AGTATTGCTG GTGGAGAAGG TTCTCCACCA 
TTCAAAGACA ATAGTGATGC GATATATATT TCAAGAATAA CAGATGGCGG CGTAGCACAA 
AAAGATGGTA AATTATTAGT TGGAGACAAA GTAATATCTA TTAATGGAGT TGAGATGAGA 
GGAGCTAAGC ATGAACAAGC AGTTGCTTTA TTAACAGGTT TAGAAAGATT TGTTCGATTA 
GTAGTTGAAC GCGAAATACC ATTTTCGCAA GCAAATGCAG CTACGGTCGT AACACCTTCA 
GAAAAATCAC CACGAGTGAT CGGTGCACCT AAACCATATA CAGGATTATA TAATGCCAAT 
AGTTATATAG CAAATAAACC GGGATATACT GGATACAGAC GTTCTATAGA TGCTGATAGA 
ACATTATCTC CAAGCCCTAC TTCGAAAACG CCTCCACCTA TGAAAATTGA AACTACTGAA 
TTACCGAAAA TGAACGGTGT TACAGAATCA GGAAATATTA AAAATGTTTC TTCGATATCA 
CCAAGTCCAA CAAATAGCCA ACTACATCCA CAGCCTGCTC CTAGGCACAG CATTTCGCAA 
CCAACAATTA CACCTACAAC TACTACGAGT TCAACGCCCA CATCTTCTAC AGAACCTAGG 
TCAACCTCAC CCCAGGAAGA TATACAGGTA CCCAAGCCTA TCACCAATGA AGAATTTCAG 
GCTATGATTC CTGCTCATTT CCTTCGTCCT CCTACTTCCT CACCATCGCC AGATTCCCAT 
CAAGGCCCTA TCGTGACAGT GACAATAAAA CAGCCTGATA ATCTACCTGG AGATGTTAAT 
TTTCCTCCGG CTCCTACAAC ACTTGGTAAA GTTACTGAGA CCATCACAAA GAGCACTCTC 
ACCGAGACCG TCGTAACTAG GGTGACTGAA AATCAATTGG TACCACCAGT AATTATTGAG 
GACGTGATTC TTGTAAAAGA GGGATCTCTA GGATTTAGTA TTATTGGTGG TACGGATCAT 
TCATGCACTC CATTTGGTGC AAAGGAACCT GGAATATTTA TATCTCATGT CGTGCCTGGT 
GGTATAGCTG CAAAATCTGG TAAATTAAGA ATGGGTGATA GAATACTAAA GGTAAATGGT 
ACTGATGTAA CAAAAGCTAC TCATCAAGAG GCTGTGATGG AACTTTTGCG ACCAGGAGAT 
CAAATAGTGC TTACTGTCCA ACACGATCCA CTACCCGAAA ATTATCAGCT GGTCGAAATA 
GAGTACATCC CTGTGACAGA ATTAGTTATT ACAAAAGAAC CAGGAGAGAA ACTGGGCATG 
CATATTAAAG GTGGACTTCG AGGACAAAAA GGTAATCCAC TTGATCATAC AGATGAAGGG 
GTTTTTATAT CCAAAATCAA CTCAGGTGGT GCAGCTAAAA GAGATGGAAG ACTGAAAGTT 
GGAATGAGAC TATTAGAAGT TAATGGTACA TCATTATTAG GCGCAACTCA CCAAGAAGCA 
GTAAATATAT TGCGATGTTC AGGAAATACG ATTACTTTGG TGGTTTGTAA AGGATATGAT 
AAGAGTGAAA TTGAACCAGT ATTACCAATA TCCGATGGAA GAGATTCTAA GGAATCTAGA 
ACATCCAAAG AATTAAAGGA TGCTACAACA GAAGGTACTA AATCGTTATC ACAAAGTATA 
TCCAGTCTGG ATCGCGATGA TGAAGAAGCA GCAACTCTTA GACAAGAACA GGAAATGAAA 
GCTGAACTTG TCGCATGGGA ACAAGAAGAA AGAGAACGTG CTTTGCTTGA GCATAGAGAA 
AAATCTACTC CTGAAAAAGT ATTAGATGTA GTAAGAGCAG CTGAATCATT AGTAAGCAAA 
TCAAATAGTC CGGTTGATAT GGTCGTACCA CCAAAGTCAC CAGGTGGTAC GAAAGATCTC 
AAAACAACCA CTATTGTGAT GAGCAAACAT ACTCTAGCAC CTCAAAATAC AAATTCACTG 
AGGAAAGAGA GAACTGGAAC ATCAGTGGAC AATCCATCGC CCAAGTCTCC GATCTCTACT 
TTAACTCCAT CGATGAATTT CGATCACACT ACTGCTGTCC AAAGCTTGCC CTCTCCAAAG 
AAAAAAAGTT CCATACCAAA ACGTAGTATT ACATTTGCTG ATCTTCCTCC CATTTCTAAA 
GAACCAATTA ATTATTCCAT TTCGGCGAAA GAAGATCCAT CACCTTTGCA TGATAGTAAA 
TTTACTTTCG ATCCTCAAAC TATTTCTTAT AAGAAAGTCT ATCAAAATCC TATCCAAGCT 
ACTCATTCTC GTTTCAAACT TCCTCCTACA CCTTTAACTG CTCCTAAATC TATAGAAGAT 
TATGGTACTT CAATTTCTTT TGGTAAACGA CAGAACGCAC GCTCTGTTGA TGGGAATAGT 
CAGGTTGAGC TTTCTCCAAC AACATCACCA ACACCACCTC TAGTAAAGAT GTCGGTTAGT 
GATAAAAAAA AATTGTTTGA AAGTGCCATG GAGGAACATT TAAAACCTTC CCCTAAACCA 
GAAAAAGTAT TCAGTTTTCT AAGTCAGGAT GAAGTTGAGA AAATGAAACA AGAAGAAGAA 
AAGAAGATTG CTACTCTAAC GCGGGATGAA TTAAAGTCGT GGGCACAACT TGATGAAAAT 
GAAGGTTTGG AAGATTTAGA AGAAACATTA GAAGATCAGG ATAATCGGCG ACCTAATAGC 
CGACTGAGTT CGCGAACTTC GATCCCTCTT CTGCAAAATC TTCCAAGCAG TGTAAGGACA 
GCAAAAGCAG AACGCCGCTT GAAAGAAAGA TTAATACAGG AGGGTATAAT TTCGGATGAA 
GATGAAGAAA GTCATTTAAG TCCTGCCGAA CAGAGAGCGT TAAGAGCAGA AAAACGTGCG 
GCATGGAGAC AAGCACGTTT GAAATCTCTG GAACAAGATG CTCTTCAAGC ACAGATAGTA 
ATTAAAAAAA TGAGTGAAAT GATGGACACG AATAAAACAA GTTCAATGCA GGATTCTACA 
GATATTCCTA TTACTCCTGT TTCTGAATCG GAAAAGACAA CTGATTTCAC TACGTTACGG 
CCGTCTAGTG CAGATTTTCC CAAACTTGCT GTACGTAGCA AAGTGGGTCC CCCTAAAGAG 
ATACGCGAAT CCGAGAAAGT AGTGGACGAG AAGGTCACTC GACGTACTGA GGAATATGTG 
GATGAGGTGA CGGGTGAACG ACGTGTGCGA ACAGTCGAAT ACGTCGAGAA ACTCATTGAA 
CGACAGGTTG AAACTCTACG AGAAAAGATA ATATCATTGG AATTAAGTAA TGCAGAAGAT 
GAAATAGAAA GTATTACCGG TACAGGAGCA AGTGATGCTG AAAGCGAAAG CGAAGAAATT 
ACTGTACAAC CTCCTACTAT GACTACTTTA CAAGAAAGAA ATGAAACAAT TATATCTACA 
AATGAAGGTG CCGCACCTAA AATAAGTGCA AATACTGTTC TTGTTTCAAA AAAAAAGAAA 
CGAAAACGTT CGAAGAAAGG TCGTCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016921379.1
CDS	1476..7625
Translation	MFRCIPIFKGCNRQVEYIDKRHCSLPCVPDDILRYSRSLEELLLDANHIRDLPKNFFRLQKLRKLGLSDNEIHRLPPDIQNFENLVELDVSRNDIPDIPENIKNLRALQVADFSSNPIPRLPAGFVQLRNLTVLGLNDMSLTNLPPDFGSLEALQSLELRENLLKSLPESLSQLYKLERLDLGDNDIEVLPAHIGELPALQELWLDHNQLQHLPPEIGELKTLVCLDVSENRLEDLPEEIGGLEALTDLHLSQNVIEKLPDGLGELKKLTILKVDQNRLSTLNPNIGRCENLQELILTENFLLELPVSIGKLLNLNNLNVDRNSLQSLPTETGNLKQLGVLSLRDNKLQYLPIEVGQCTALHVLDVSGNRLQYLPYSLINLNLKAVWLSKNQAQPMLTFQTDVDEETGQEVLTCFLLPQLEIHPDDSGRLGMLAGMRNAVQDGEIRELGSSDDEGWQEKEASRTHSVKFTDEPPETDKETPFVRQNTPHPKELKAKAHKLFSKGKNDSRSASTDEQDVSQTFGLDKTETDVSTKPNDLTVDLIEQESLKHESVENEQKEIVPGVTDNIDVQSSNEPEIVSNQYIVESNTDVRTEGSISELKDRNDKEDDESENEETQRHVEFSIAEGTDYDGSGDSNRPNRLHRRDTPHHLKNKRIHTAVDKEKVASIIAQALMKKNDEISTSTSAPAETTENFDAQSQDAEPTIEVREEQYEIHIERTTGGLGLSIAGGIGSTPFKGDDEGIFISRVTEGGPADLAGLKVEDKVLSVNGVSVVNVGHYDAVEVLKACGRVLVLVVQREVTRIVPPFEQISSRKNSVCSSLSTSRAPSATSHVSSIGFSHNLENGDALSHDAIKCKKIPEPISSTKVGNEPMVSVLVHTTLIRDQNGLGFSIAGGEGSPPFKDNSDAIYISRITDGGVAQKDGKLLVGDKVISINGVEMRGAKHEQAVALLTGLERFVRLVVEREIPFSQANAATVVTPSEKSPRVIGAPKPYTGLYNANSYIANKPGYTGYRRSIDADRTLSPSPTSKTPPPMKIETTELPKMNGVTESGNIKNVSSISPSPTNSQLHPQPAPRHSISQPTITPTTTTSSTPTSSTEPRSTSPQEDIQVPKPITNEEFQAMIPAHFLRPPTSSPSPDSHQGPIVTVTIKQPDNLPGDVNFPPAPTTLGKVTETITKSTLTETVVTRVTENQLVPPVIIEDVILVKEGSLGFSIIGGTDHSCTPFGAKEPGIFISHVVPGGIAAKSGKLRMGDRILKVNGTDVTKATHQEAVMELLRPGDQIVLTVQHDPLPENYQLVEIEYIPVTELVITKEPGEKLGMHIKGGLRGQKGNPLDHTDEGVFISKINSGGAAKRDGRLKVGMRLLEVNGTSLLGATHQEAVNILRCSGNTITLVVCKGYDKSEIEPVLPISDGRDSKESRTSKELKDATTEGTKSLSQSISSLDRDDEEAATLRQEQEMKAELVAWEQEERERALLEHREKSTPEKVLDVVRAAESLVSKSNSPVDMVVPPKSPGGTKDLKTTTIVMSKHTLAPQNTNSLRKERTGTSVDNPSPKSPISTLTPSMNFDHTTAVQSLPSPKKKSSIPKRSITFADLPPISKEPINYSISAKEDPSPLHDSKFTFDPQTISYKKVYQNPIQATHSRFKLPPTPLTAPKSIEDYGTSISFGKRQNARSVDGNSQVELSPTTSPTPPLVKMSVSDKKKLFESAMEEHLKPSPKPEKVFSFLSQDEVEKMKQEEEKKIATLTRDELKSWAQLDENEGLEDLEETLEDQDNRRPNSRLSSRTSIPLLQNLPSSVRTAKAERRLKERLIQEGIISDEDEESHLSPAEQRALRAEKRAAWRQARLKSLEQDALQAQIVIKKMSEMMDTNKTSSMQDSTDIPITPVSESEKTTDFTTLRPSSADFPKLAVRSKVGPPKEIRESEKVVDEKVTRRTEEYVDEVTGERRVRTVEYVEKLIERQVETLREKIISLELSNAEDEIESITGTGASDAESESEEITVQPPTMTTLQERNETIISTNEGAAPKISANTVLVSKKKKRKRSKKGRH

Target ORF information:

RefSeq Version	XM_017065890.2
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana protein lap4 (LOC108003564), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017065890.2

ORF Insert Sequence:

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCTGAGCCGA CAATAGAAGT ACGAGAAGAA CAATACGAAA TTCACATCGA AAGAACAACA 
GGTGGTCTAG GCTTATCAAT AGCTGGTGGT ATTGGTTCAA CCCCTTTTAA AGGCGATGAT 
GAAGGCATTT TTATTTCCAG AGTTACTGAA GGTGGACCTG CAGATTTAGC AGGTTTAAAA 
GTGGAAGATA AAGTATTATC TGTTAATGGT GTATCAGTAG TAAATGTAGG TCATTATGAT 
GCTGTAGAAG TTTTGAAAGC TTGTGGACGA GTTCTTGTAT TAGTAGTTCA AAGAGAAGTT 
ACAAGGATAG TGCCTCCATT TGAACAGATA TCATCGAGAA AAAACTCAGT GTGCTCTAGT 
TTAAGTACTA GTAGAGCTCC AAGTGCAACA TCTCATGTTT CATCTATAGG TTTTTCACAT 
AATTTAGAAA ATGGTGATGC TTTATCTCAT GATGCTATTA AGTGTAAGAA AATTCCTGAA 
CCTATATCTT CAACAAAAGT AGGCAATGAA CCAATGGTAT CAGTTCTTGT TCACACAACA 
TTAATACGGG ACCAAAATGG GCTTGGATTT AGTATTGCTG GTGGAGAAGG TTCTCCACCA 
TTCAAAGACA ATAGTGATGC GATATATATT TCAAGAATAA CAGATGGCGG CGTAGCACAA 
AAAGATGGTA AATTATTAGT TGGAGACAAA GTAATATCTA TTAATGGAGT TGAGATGAGA 
GGAGCTAAGC ATGAACAAGC AGTTGCTTTA TTAACAGGTT TAGAAAGATT TGTTCGATTA 
GTAGTTGAAC GCGAAATACC ATTTTCGCAA GCAAATGCAG CTACGGTCGT AACACCTTCA 
GAAAAATCAC CACGAGTGAT CGGTGCACCT AAACCATATA CAGGATTATA TAATGCCAAT 
AGTTATATAG CAAATAAACC GGGATATACT GGATACAGAC GTTCTATAGA TGCTGATAGA 
ACATTATCTC CAAGCCCTAC TTCGAAAACG CCTCCACCTA TGAAAATTGA AACTACTGAA 
TTACCGAAAA TGAACGGTGT TACAGAATCA GGAAATATTA AAAATGTTTC TTCGATATCA 
CCAAGTCCAA CAAATAGCCA ACTACATCCA CAGCCTGCTC CTAGGCACAG CATTTCGCAA 
CCAACAATTA CACCTACAAC TACTACGAGT TCAACGCCCA CATCTTCTAC AGAACCTAGG 
TCAACCTCAC CCCAGGAAGA TATACAGGTA CCCAAGCCTA TCACCAATGA AGAATTTCAG 
GCTATGATTC CTGCTCATTT CCTTCGTCCT CCTACTTCCT CACCATCGCC AGATTCCCAT 
CAAGGCCCTA TCGTGACAGT GACAATAAAA CAGCCTGATA ATCTACCTGG AGATGTTAAT 
TTTCCTCCGG CTCCTACAAC ACTTGGTAAA GTTACTGAGA CCATCACAAA GAGCACTCTC 
ACCGAGACCG TCGTAACTAG GGTGACTGAA AATCAATTGG TACCACCAGT AATTATTGAG 
GACGTGATTC TTGTAAAAGA GGGATCTCTA GGATTTAGTA TTATTGGTGG TACGGATCAT 
TCATGCACTC CATTTGGTGC AAAGGAACCT GGAATATTTA TATCTCATGT CGTGCCTGGT 
GGTATAGCTG CAAAATCTGG TAAATTAAGA ATGGGTGATA GAATACTAAA GGTAAATGGT 
ACTGATGTAA CAAAAGCTAC TCATCAAGAG GCTGTGATGG AACTTTTGCG ACCAGGAGAT 
CAAATAGTGC TTACTGTCCA ACACGATCCA CTACCCGAAA ATTATCAGCT GGTCGAAATA 
GAGTACATCC CTGTGACAGA ATTAGTTATT ACAAAAGAAC CAGGAGAGAA ACTGGGCATG 
CATATTAAAG GTGGACTTCG AGGACAAAAA GGTAATCCAC TTGATCATAC AGATGAAGGG 
GTTTTTATAT CCAAAATCAA CTCAGGTGGT GCAGCTAAAA GAGATGGAAG ACTGAAAGTT 
GGAATGAGAC TATTAGAAGT TAATGGTACA TCATTATTAG GCGCAACTCA CCAAGAAGCA 
GTAAATATAT TGCGATGTTC AGGAAATACG ATTACTTTGG TGGTTTGTAA AGGATATGAT 
AAGAGTGAAA TTGAACCAGT ATTACCAATA TCCGATGGAA GAGATTCTAA GGAATCTAGA 
ACATCCAAAG AATTAAAGGA TGCTACAACA GAAGGTACTA AATCGTTATC ACAAAGTATA 
TCCAGTCTGG ATCGCGATGA TGAAGAAGCA GCAACTCTTA GACAAGAACA GGAAATGAAA 
GCTGAACTTG TCGCATGGGA ACAAGAAGAA AGAGAACGTG CTTTGCTTGA GCATAGAGAA 
AAATCTACTC CTGAAAAAGT ATTAGATGTA GTAAGAGCAG CTGAATCATT AGTAAGCAAA 
TCAAATAGTC CGGTTGATAT GGTCGTACCA CCAAAGTCAC CAGGTGGTAC GAAAGATCTC 
AAAACAACCA CTATTGTGAT GAGCAAACAT ACTCTAGCAC CTCAAAATAC AAATTCACTG 
AGGAAAGAGA GAACTGGAAC ATCAGTGGAC AATCCATCGC CCAAGTCTCC GATCTCTACT 
TTAACTCCAT CGATGAATTT CGATCACACT ACTGCTGTCC AAAGCTTGCC CTCTCCAAAG 
AAAAAAAGTT CCATACCAAA ACGTAGTATT ACATTTGCTG ATCTTCCTCC CATTTCTAAA 
GAACCAATTA ATTATTCCAT TTCGGCGAAA GAAGATCCAT CACCTTTGCA TGATAGTAAA 
TTTACTTTCG ATCCTCAAAC TATTTCTTAT AAGAAAGTCT ATCAAAATCC TATCCAAGCT 
ACTCATTCTC GTTTCAAACT TCCTCCTACA CCTTTAACTG CTCCTAAATC TATAGAAGAT 
TATGGTACTT CAATTTCTTT TGGTAAACGA CAGAACGCAC GCTCTGTTGA TGGGAATAGT 
CAGGTTGAGC TTTCTCCAAC AACATCACCA ACACCACCTC TAGTAAAGAT GTCGGTTAGT 
GATAAAAAAA AATTGTTTGA AAGTGCCATG GAGGAACATT TAAAACCTTC CCCTAAACCA 
GAAAAAGTAT TCAGTTTTCT AAGTCAGGAT GAAGTTGAGA AAATGAAACA AGAAGAAGAA 
AAGAAGATTG CTACTCTAAC GCGGGATGAA TTAAAGTCGT GGGCACAACT TGATGAAAAT 
GAAGGTTTGG AAGATTTAGA AGAAACATTA GAAGATCAGG ATAATCGGCG ACCTAATAGC 
CGACTGAGTT CGCGAACTTC GATCCCTCTT CTGCAAAATC TTCCAAGCAG TGTAAGGACA 
GCAAAAGCAG AACGCCGCTT GAAAGAAAGA TTAATACAGG AGGGTATAAT TTCGGATGAA 
GATGAAGAAA GTCATTTAAG TCCTGCCGAA CAGAGAGCGT TAAGAGCAGA AAAACGTGCG 
GCATGGAGAC AAGCACGTTT GAAATCTCTG GAACAAGATG CTCTTCAAGC ACAGATAGTA 
ATTAAAAAAA TGAGTGAAAT GATGGACACG AATAAAACAA GTTCAATGCA GGATTCTACA 
GATATTCCTA TTACTCCTGT TTCTGAATCG GAAAAGACAA CTGATTTCAC TACGTTACGG 
CCGTCTAGTG CAGATTTTCC CAAACTTGCT GTACGTAGCA AAGTGGGTCC CCCTAAAGAG 
ATACGCGAAT CCGAGAAAGT AGTGGACGAG AAGGTCACTC GACGTACTGA GGAATATGTG 
GATGAGGTGA CGGGTGAACG ACGTGTGCGA ACAGTCGAAT ACGTCGAGAA ACTCATTGAA 
CGACAGGTTG AAACTCTACG AGAAAAGATA ATATCATTGG AATTAAGTAA TGCAGAAGAT 
GAAATAGAAA GTATTACCGG TACAGGAGCA AGTGATGCTG AAAGCGAAAG CGAAGAAATT 
ACTGTACAAC CTCCTACTAT GACTACTTTA CAAGAAAGAA ATGAAACAAT TATATCTACA 
AATGAAGGTG CCGCACCTAA AATAAGTGCA AATACTGTTC TTGTTTCAAA AAAAAAGAAA 
CGAAAACGTT CGAAGAAAGG TCGTCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108003564 ( XM_017065890.1 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_016921379.1 Apis cerana protein lap4 (LOC108003564), transcript variant X4, mRNA.

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CloneID	OAp29724
Clone ID Related Accession (Same CDS sequence)	XM_017065890.2 , XM_017065890.1
Accession Version	XM_017065890.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-08
Organism	Apis cerana(Asiatic honeybee)
Product	protein lap4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016017567.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis cerana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCTGAGCCGA CAATAGAAGT ACGAGAAGAA CAATACGAAA TTCACATCGA AAGAACAACA 
GGTGGTCTAG GCTTATCAAT AGCTGGTGGT ATTGGTTCAA CCCCTTTTAA AGGCGATGAT 
GAAGGCATTT TTATTTCCAG AGTTACTGAA GGTGGACCTG CAGATTTAGC AGGTTTAAAA 
GTGGAAGATA AAGTATTATC TGTTAATGGT GTATCAGTAG TAAATGTAGG TCATTATGAT 
GCTGTAGAAG TTTTGAAAGC TTGTGGACGA GTTCTTGTAT TAGTAGTTCA AAGAGAAGTT 
ACAAGGATAG TGCCTCCATT TGAACAGATA TCATCGAGAA AAAACTCAGT GTGCTCTAGT 
TTAAGTACTA GTAGAGCTCC AAGTGCAACA TCTCATGTTT CATCTATAGG TTTTTCACAT 
AATTTAGAAA ATGGTGATGC TTTATCTCAT GATGCTATTA AGTGTAAGAA AATTCCTGAA 
CCTATATCTT CAACAAAAGT AGGCAATGAA CCAATGGTAT CAGTTCTTGT TCACACAACA 
TTAATACGGG ACCAAAATGG GCTTGGATTT AGTATTGCTG GTGGAGAAGG TTCTCCACCA 
TTCAAAGACA ATAGTGATGC GATATATATT TCAAGAATAA CAGATGGCGG CGTAGCACAA 
AAAGATGGTA AATTATTAGT TGGAGACAAA GTAATATCTA TTAATGGAGT TGAGATGAGA 
GGAGCTAAGC ATGAACAAGC AGTTGCTTTA TTAACAGGTT TAGAAAGATT TGTTCGATTA 
GTAGTTGAAC GCGAAATACC ATTTTCGCAA GCAAATGCAG CTACGGTCGT AACACCTTCA 
GAAAAATCAC CACGAGTGAT CGGTGCACCT AAACCATATA CAGGATTATA TAATGCCAAT 
AGTTATATAG CAAATAAACC GGGATATACT GGATACAGAC GTTCTATAGA TGCTGATAGA 
ACATTATCTC CAAGCCCTAC TTCGAAAACG CCTCCACCTA TGAAAATTGA AACTACTGAA 
TTACCGAAAA TGAACGGTGT TACAGAATCA GGAAATATTA AAAATGTTTC TTCGATATCA 
CCAAGTCCAA CAAATAGCCA ACTACATCCA CAGCCTGCTC CTAGGCACAG CATTTCGCAA 
CCAACAATTA CACCTACAAC TACTACGAGT TCAACGCCCA CATCTTCTAC AGAACCTAGG 
TCAACCTCAC CCCAGGAAGA TATACAGGTA CCCAAGCCTA TCACCAATGA AGAATTTCAG 
GCTATGATTC CTGCTCATTT CCTTCGTCCT CCTACTTCCT CACCATCGCC AGATTCCCAT 
CAAGGCCCTA TCGTGACAGT GACAATAAAA CAGCCTGATA ATCTACCTGG AGATGTTAAT 
TTTCCTCCGG CTCCTACAAC ACTTGGTAAA GTTACTGAGA CCATCACAAA GAGCACTCTC 
ACCGAGACCG TCGTAACTAG GGTGACTGAA AATCAATTGG TACCACCAGT AATTATTGAG 
GACGTGATTC TTGTAAAAGA GGGATCTCTA GGATTTAGTA TTATTGGTGG TACGGATCAT 
TCATGCACTC CATTTGGTGC AAAGGAACCT GGAATATTTA TATCTCATGT CGTGCCTGGT 
GGTATAGCTG CAAAATCTGG TAAATTAAGA ATGGGTGATA GAATACTAAA GGTAAATGGT 
ACTGATGTAA CAAAAGCTAC TCATCAAGAG GCTGTGATGG AACTTTTGCG ACCAGGAGAT 
CAAATAGTGC TTACTGTCCA ACACGATCCA CTACCCGAAA ATTATCAGCT GGTCGAAATA 
GAGTACATCC CTGTGACAGA ATTAGTTATT ACAAAAGAAC CAGGAGAGAA ACTGGGCATG 
CATATTAAAG GTGGACTTCG AGGACAAAAA GGTAATCCAC TTGATCATAC AGATGAAGGG 
GTTTTTATAT CCAAAATCAA CTCAGGTGGT GCAGCTAAAA GAGATGGAAG ACTGAAAGTT 
GGAATGAGAC TATTAGAAGT TAATGGTACA TCATTATTAG GCGCAACTCA CCAAGAAGCA 
GTAAATATAT TGCGATGTTC AGGAAATACG ATTACTTTGG TGGTTTGTAA AGGATATGAT 
AAGAGTGAAA TTGAACCAGT ATTACCAATA TCCGATGGAA GAGATTCTAA GGAATCTAGA 
ACATCCAAAG AATTAAAGGA TGCTACAACA GAAGGTACTA AATCGTTATC ACAAAGTATA 
TCCAGTCTGG ATCGCGATGA TGAAGAAGCA GCAACTCTTA GACAAGAACA GGAAATGAAA 
GCTGAACTTG TCGCATGGGA ACAAGAAGAA AGAGAACGTG CTTTGCTTGA GCATAGAGAA 
AAATCTACTC CTGAAAAAGT ATTAGATGTA GTAAGAGCAG CTGAATCATT AGTAAGCAAA 
TCAAATAGTC CGGTTGATAT GGTCGTACCA CCAAAGTCAC CAGGTGGTAC GAAAGATCTC 
AAAACAACCA CTATTGTGAT GAGCAAACAT ACTCTAGCAC CTCAAAATAC AAATTCACTG 
AGGAAAGAGA GAACTGGAAC ATCAGTGGAC AATCCATCGC CCAAGTCTCC GATCTCTACT 
TTAACTCCAT CGATGAATTT CGATCACACT ACTGCTGTCC AAAGCTTGCC CTCTCCAAAG 
AAAAAAAGTT CCATACCAAA ACGTAGTATT ACATTTGCTG ATCTTCCTCC CATTTCTAAA 
GAACCAATTA ATTATTCCAT TTCGGCGAAA GAAGATCCAT CACCTTTGCA TGATAGTAAA 
TTTACTTTCG ATCCTCAAAC TATTTCTTAT AAGAAAGTCT ATCAAAATCC TATCCAAGCT 
ACTCATTCTC GTTTCAAACT TCCTCCTACA CCTTTAACTG CTCCTAAATC TATAGAAGAT 
TATGGTACTT CAATTTCTTT TGGTAAACGA CAGAACGCAC GCTCTGTTGA TGGGAATAGT 
CAGGTTGAGC TTTCTCCAAC AACATCACCA ACACCACCTC TAGTAAAGAT GTCGGTTAGT 
GATAAAAAAA AATTGTTTGA AAGTGCCATG GAGGAACATT TAAAACCTTC CCCTAAACCA 
GAAAAAGTAT TCAGTTTTCT AAGTCAGGAT GAAGTTGAGA AAATGAAACA AGAAGAAGAA 
AAGAAGATTG CTACTCTAAC GCGGGATGAA TTAAAGTCGT GGGCACAACT TGATGAAAAT 
GAAGGTTTGG AAGATTTAGA AGAAACATTA GAAGATCAGG ATAATCGGCG ACCTAATAGC 
CGACTGAGTT CGCGAACTTC GATCCCTCTT CTGCAAAATC TTCCAAGCAG TGTAAGGACA 
GCAAAAGCAG AACGCCGCTT GAAAGAAAGA TTAATACAGG AGGGTATAAT TTCGGATGAA 
GATGAAGAAA GTCATTTAAG TCCTGCCGAA CAGAGAGCGT TAAGAGCAGA AAAACGTGCG 
GCATGGAGAC AAGCACGTTT GAAATCTCTG GAACAAGATG CTCTTCAAGC ACAGATAGTA 
ATTAAAAAAA TGAGTGAAAT GATGGACACG AATAAAACAA GTTCAATGCA GGATTCTACA 
GATATTCCTA TTACTCCTGT TTCTGAATCG GAAAAGACAA CTGATTTCAC TACGTTACGG 
CCGTCTAGTG CAGATTTTCC CAAACTTGCT GTACGTAGCA AAGTGGGTCC CCCTAAAGAG 
ATACGCGAAT CCGAGAAAGT AGTGGACGAG AAGGTCACTC GACGTACTGA GGAATATGTG 
GATGAGGTGA CGGGTGAACG ACGTGTGCGA ACAGTCGAAT ACGTCGAGAA ACTCATTGAA 
CGACAGGTTG AAACTCTACG AGAAAAGATA ATATCATTGG AATTAAGTAA TGCAGAAGAT 
GAAATAGAAA GTATTACCGG TACAGGAGCA AGTGATGCTG AAAGCGAAAG CGAAGAAATT 
ACTGTACAAC CTCCTACTAT GACTACTTTA CAAGAAAGAA ATGAAACAAT TATATCTACA 
AATGAAGGTG CCGCACCTAA AATAAGTGCA AATACTGTTC TTGTTTCAAA AAAAAAGAAA 
CGAAAACGTT CGAAGAAAGG TCGTCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016921379.1
CDS	233..6382
Translation	MFRCIPIFKGCNRQVEYIDKRHCSLPCVPDDILRYSRSLEELLLDANHIRDLPKNFFRLQKLRKLGLSDNEIHRLPPDIQNFENLVELDVSRNDIPDIPENIKNLRALQVADFSSNPIPRLPAGFVQLRNLTVLGLNDMSLTNLPPDFGSLEALQSLELRENLLKSLPESLSQLYKLERLDLGDNDIEVLPAHIGELPALQELWLDHNQLQHLPPEIGELKTLVCLDVSENRLEDLPEEIGGLEALTDLHLSQNVIEKLPDGLGELKKLTILKVDQNRLSTLNPNIGRCENLQELILTENFLLELPVSIGKLLNLNNLNVDRNSLQSLPTETGNLKQLGVLSLRDNKLQYLPIEVGQCTALHVLDVSGNRLQYLPYSLINLNLKAVWLSKNQAQPMLTFQTDVDEETGQEVLTCFLLPQLEIHPDDSGRLGMLAGMRNAVQDGEIRELGSSDDEGWQEKEASRTHSVKFTDEPPETDKETPFVRQNTPHPKELKAKAHKLFSKGKNDSRSASTDEQDVSQTFGLDKTETDVSTKPNDLTVDLIEQESLKHESVENEQKEIVPGVTDNIDVQSSNEPEIVSNQYIVESNTDVRTEGSISELKDRNDKEDDESENEETQRHVEFSIAEGTDYDGSGDSNRPNRLHRRDTPHHLKNKRIHTAVDKEKVASIIAQALMKKNDEISTSTSAPAETTENFDAQSQDAEPTIEVREEQYEIHIERTTGGLGLSIAGGIGSTPFKGDDEGIFISRVTEGGPADLAGLKVEDKVLSVNGVSVVNVGHYDAVEVLKACGRVLVLVVQREVTRIVPPFEQISSRKNSVCSSLSTSRAPSATSHVSSIGFSHNLENGDALSHDAIKCKKIPEPISSTKVGNEPMVSVLVHTTLIRDQNGLGFSIAGGEGSPPFKDNSDAIYISRITDGGVAQKDGKLLVGDKVISINGVEMRGAKHEQAVALLTGLERFVRLVVEREIPFSQANAATVVTPSEKSPRVIGAPKPYTGLYNANSYIANKPGYTGYRRSIDADRTLSPSPTSKTPPPMKIETTELPKMNGVTESGNIKNVSSISPSPTNSQLHPQPAPRHSISQPTITPTTTTSSTPTSSTEPRSTSPQEDIQVPKPITNEEFQAMIPAHFLRPPTSSPSPDSHQGPIVTVTIKQPDNLPGDVNFPPAPTTLGKVTETITKSTLTETVVTRVTENQLVPPVIIEDVILVKEGSLGFSIIGGTDHSCTPFGAKEPGIFISHVVPGGIAAKSGKLRMGDRILKVNGTDVTKATHQEAVMELLRPGDQIVLTVQHDPLPENYQLVEIEYIPVTELVITKEPGEKLGMHIKGGLRGQKGNPLDHTDEGVFISKINSGGAAKRDGRLKVGMRLLEVNGTSLLGATHQEAVNILRCSGNTITLVVCKGYDKSEIEPVLPISDGRDSKESRTSKELKDATTEGTKSLSQSISSLDRDDEEAATLRQEQEMKAELVAWEQEERERALLEHREKSTPEKVLDVVRAAESLVSKSNSPVDMVVPPKSPGGTKDLKTTTIVMSKHTLAPQNTNSLRKERTGTSVDNPSPKSPISTLTPSMNFDHTTAVQSLPSPKKKSSIPKRSITFADLPPISKEPINYSISAKEDPSPLHDSKFTFDPQTISYKKVYQNPIQATHSRFKLPPTPLTAPKSIEDYGTSISFGKRQNARSVDGNSQVELSPTTSPTPPLVKMSVSDKKKLFESAMEEHLKPSPKPEKVFSFLSQDEVEKMKQEEEKKIATLTRDELKSWAQLDENEGLEDLEETLEDQDNRRPNSRLSSRTSIPLLQNLPSSVRTAKAERRLKERLIQEGIISDEDEESHLSPAEQRALRAEKRAAWRQARLKSLEQDALQAQIVIKKMSEMMDTNKTSSMQDSTDIPITPVSESEKTTDFTTLRPSSADFPKLAVRSKVGPPKEIRESEKVVDEKVTRRTEEYVDEVTGERRVRTVEYVEKLIERQVETLREKIISLELSNAEDEIESITGTGASDAESESEEITVQPPTMTTLQERNETIISTNEGAAPKISANTVLVSKKKKRKRSKKGRH

Target ORF information:

RefSeq Version	XM_017065890.1
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana protein lap4 (LOC108003564), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017065890.1

ORF Insert Sequence:

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCTGAGCCGA CAATAGAAGT ACGAGAAGAA CAATACGAAA TTCACATCGA AAGAACAACA 
GGTGGTCTAG GCTTATCAAT AGCTGGTGGT ATTGGTTCAA CCCCTTTTAA AGGCGATGAT 
GAAGGCATTT TTATTTCCAG AGTTACTGAA GGTGGACCTG CAGATTTAGC AGGTTTAAAA 
GTGGAAGATA AAGTATTATC TGTTAATGGT GTATCAGTAG TAAATGTAGG TCATTATGAT 
GCTGTAGAAG TTTTGAAAGC TTGTGGACGA GTTCTTGTAT TAGTAGTTCA AAGAGAAGTT 
ACAAGGATAG TGCCTCCATT TGAACAGATA TCATCGAGAA AAAACTCAGT GTGCTCTAGT 
TTAAGTACTA GTAGAGCTCC AAGTGCAACA TCTCATGTTT CATCTATAGG TTTTTCACAT 
AATTTAGAAA ATGGTGATGC TTTATCTCAT GATGCTATTA AGTGTAAGAA AATTCCTGAA 
CCTATATCTT CAACAAAAGT AGGCAATGAA CCAATGGTAT CAGTTCTTGT TCACACAACA 
TTAATACGGG ACCAAAATGG GCTTGGATTT AGTATTGCTG GTGGAGAAGG TTCTCCACCA 
TTCAAAGACA ATAGTGATGC GATATATATT TCAAGAATAA CAGATGGCGG CGTAGCACAA 
AAAGATGGTA AATTATTAGT TGGAGACAAA GTAATATCTA TTAATGGAGT TGAGATGAGA 
GGAGCTAAGC ATGAACAAGC AGTTGCTTTA TTAACAGGTT TAGAAAGATT TGTTCGATTA 
GTAGTTGAAC GCGAAATACC ATTTTCGCAA GCAAATGCAG CTACGGTCGT AACACCTTCA 
GAAAAATCAC CACGAGTGAT CGGTGCACCT AAACCATATA CAGGATTATA TAATGCCAAT 
AGTTATATAG CAAATAAACC GGGATATACT GGATACAGAC GTTCTATAGA TGCTGATAGA 
ACATTATCTC CAAGCCCTAC TTCGAAAACG CCTCCACCTA TGAAAATTGA AACTACTGAA 
TTACCGAAAA TGAACGGTGT TACAGAATCA GGAAATATTA AAAATGTTTC TTCGATATCA 
CCAAGTCCAA CAAATAGCCA ACTACATCCA CAGCCTGCTC CTAGGCACAG CATTTCGCAA 
CCAACAATTA CACCTACAAC TACTACGAGT TCAACGCCCA CATCTTCTAC AGAACCTAGG 
TCAACCTCAC CCCAGGAAGA TATACAGGTA CCCAAGCCTA TCACCAATGA AGAATTTCAG 
GCTATGATTC CTGCTCATTT CCTTCGTCCT CCTACTTCCT CACCATCGCC AGATTCCCAT 
CAAGGCCCTA TCGTGACAGT GACAATAAAA CAGCCTGATA ATCTACCTGG AGATGTTAAT 
TTTCCTCCGG CTCCTACAAC ACTTGGTAAA GTTACTGAGA CCATCACAAA GAGCACTCTC 
ACCGAGACCG TCGTAACTAG GGTGACTGAA AATCAATTGG TACCACCAGT AATTATTGAG 
GACGTGATTC TTGTAAAAGA GGGATCTCTA GGATTTAGTA TTATTGGTGG TACGGATCAT 
TCATGCACTC CATTTGGTGC AAAGGAACCT GGAATATTTA TATCTCATGT CGTGCCTGGT 
GGTATAGCTG CAAAATCTGG TAAATTAAGA ATGGGTGATA GAATACTAAA GGTAAATGGT 
ACTGATGTAA CAAAAGCTAC TCATCAAGAG GCTGTGATGG AACTTTTGCG ACCAGGAGAT 
CAAATAGTGC TTACTGTCCA ACACGATCCA CTACCCGAAA ATTATCAGCT GGTCGAAATA 
GAGTACATCC CTGTGACAGA ATTAGTTATT ACAAAAGAAC CAGGAGAGAA ACTGGGCATG 
CATATTAAAG GTGGACTTCG AGGACAAAAA GGTAATCCAC TTGATCATAC AGATGAAGGG 
GTTTTTATAT CCAAAATCAA CTCAGGTGGT GCAGCTAAAA GAGATGGAAG ACTGAAAGTT 
GGAATGAGAC TATTAGAAGT TAATGGTACA TCATTATTAG GCGCAACTCA CCAAGAAGCA 
GTAAATATAT TGCGATGTTC AGGAAATACG ATTACTTTGG TGGTTTGTAA AGGATATGAT 
AAGAGTGAAA TTGAACCAGT ATTACCAATA TCCGATGGAA GAGATTCTAA GGAATCTAGA 
ACATCCAAAG AATTAAAGGA TGCTACAACA GAAGGTACTA AATCGTTATC ACAAAGTATA 
TCCAGTCTGG ATCGCGATGA TGAAGAAGCA GCAACTCTTA GACAAGAACA GGAAATGAAA 
GCTGAACTTG TCGCATGGGA ACAAGAAGAA AGAGAACGTG CTTTGCTTGA GCATAGAGAA 
AAATCTACTC CTGAAAAAGT ATTAGATGTA GTAAGAGCAG CTGAATCATT AGTAAGCAAA 
TCAAATAGTC CGGTTGATAT GGTCGTACCA CCAAAGTCAC CAGGTGGTAC GAAAGATCTC 
AAAACAACCA CTATTGTGAT GAGCAAACAT ACTCTAGCAC CTCAAAATAC AAATTCACTG 
AGGAAAGAGA GAACTGGAAC ATCAGTGGAC AATCCATCGC CCAAGTCTCC GATCTCTACT 
TTAACTCCAT CGATGAATTT CGATCACACT ACTGCTGTCC AAAGCTTGCC CTCTCCAAAG 
AAAAAAAGTT CCATACCAAA ACGTAGTATT ACATTTGCTG ATCTTCCTCC CATTTCTAAA 
GAACCAATTA ATTATTCCAT TTCGGCGAAA GAAGATCCAT CACCTTTGCA TGATAGTAAA 
TTTACTTTCG ATCCTCAAAC TATTTCTTAT AAGAAAGTCT ATCAAAATCC TATCCAAGCT 
ACTCATTCTC GTTTCAAACT TCCTCCTACA CCTTTAACTG CTCCTAAATC TATAGAAGAT 
TATGGTACTT CAATTTCTTT TGGTAAACGA CAGAACGCAC GCTCTGTTGA TGGGAATAGT 
CAGGTTGAGC TTTCTCCAAC AACATCACCA ACACCACCTC TAGTAAAGAT GTCGGTTAGT 
GATAAAAAAA AATTGTTTGA AAGTGCCATG GAGGAACATT TAAAACCTTC CCCTAAACCA 
GAAAAAGTAT TCAGTTTTCT AAGTCAGGAT GAAGTTGAGA AAATGAAACA AGAAGAAGAA 
AAGAAGATTG CTACTCTAAC GCGGGATGAA TTAAAGTCGT GGGCACAACT TGATGAAAAT 
GAAGGTTTGG AAGATTTAGA AGAAACATTA GAAGATCAGG ATAATCGGCG ACCTAATAGC 
CGACTGAGTT CGCGAACTTC GATCCCTCTT CTGCAAAATC TTCCAAGCAG TGTAAGGACA 
GCAAAAGCAG AACGCCGCTT GAAAGAAAGA TTAATACAGG AGGGTATAAT TTCGGATGAA 
GATGAAGAAA GTCATTTAAG TCCTGCCGAA CAGAGAGCGT TAAGAGCAGA AAAACGTGCG 
GCATGGAGAC AAGCACGTTT GAAATCTCTG GAACAAGATG CTCTTCAAGC ACAGATAGTA 
ATTAAAAAAA TGAGTGAAAT GATGGACACG AATAAAACAA GTTCAATGCA GGATTCTACA 
GATATTCCTA TTACTCCTGT TTCTGAATCG GAAAAGACAA CTGATTTCAC TACGTTACGG 
CCGTCTAGTG CAGATTTTCC CAAACTTGCT GTACGTAGCA AAGTGGGTCC CCCTAAAGAG 
ATACGCGAAT CCGAGAAAGT AGTGGACGAG AAGGTCACTC GACGTACTGA GGAATATGTG 
GATGAGGTGA CGGGTGAACG ACGTGTGCGA ACAGTCGAAT ACGTCGAGAA ACTCATTGAA 
CGACAGGTTG AAACTCTACG AGAAAAGATA ATATCATTGG AATTAAGTAA TGCAGAAGAT 
GAAATAGAAA GTATTACCGG TACAGGAGCA AGTGATGCTG AAAGCGAAAG CGAAGAAATT 
ACTGTACAAC CTCCTACTAT GACTACTTTA CAAGAAAGAA ATGAAACAAT TATATCTACA 
AATGAAGGTG CCGCACCTAA AATAAGTGCA AATACTGTTC TTGTTTCAAA AAAAAAGAAA 
CGAAAACGTT CGAAGAAAGG TCGTCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108003564 ( XM_017065891.2 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_016921380.1 Apis cerana protein lap4 (LOC108003564), transcript variant X5, mRNA.

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CloneID	OAp29725
Clone ID Related Accession (Same CDS sequence)	XM_017065891.2 , XM_017065891.1
Accession Version	XM_017065891.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-16
Organism	Apis cerana(Asiatic honeybee)
Product	protein lap4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016017567.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 17, 2019 this sequence version replaced XM_017065891.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis cerana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGC AGATATCATC GAGAAAAAAC 
TCAGTGTGCT CTAGTTTAAG TACTAGTAGA GCTCCAAGTG CAACATCTCA TGTTTCATCT 
ATAGGTTTTT CACATAATTT AGAAAATGGT GATGCTTTAT CTCATGATGC TATTAAGTGT 
AAGAAAATTC CTGAACCTAT ATCTTCAACA AAAGTAGGCA ATGAACCAAT GGTATCAGTT 
CTTGTTCACA CAACATTAAT ACGGGACCAA AATGGGCTTG GATTTAGTAT TGCTGGTGGA 
GAAGGTTCTC CACCATTCAA AGACAATAGT GATGCGATAT ATATTTCAAG AATAACAGAT 
GGCGGCGTAG CACAAAAAGA TGGTAAATTA TTAGTTGGAG ACAAAGTAAT ATCTATTAAT 
GGAGTTGAGA TGAGAGGAGC TAAGCATGAA CAAGCAGTTG CTTTATTAAC AGGTTTAGAA 
AGATTTGTTC GATTAGTAGT TGAACGCGAA ATACCATTTT CGCAAGCAAA TGCAGCTACG 
GTCGTAACAC CTTCAGAAAA ATCACCACGA GTGATCGGTG CACCTAAACC ATATACAGGA 
TTATATAATG CCAATAGTTA TATAGCAAAT AAACCGGGAT ATACTGGATA CAGACGTTCT 
ATAGATGCTG ATAGAACATT ATCTCCAAGC CCTACTTCGA AAACGCCTCC ACCTATGAAA 
ATTGAAACTA CTGAATTACC GAAAATGAAC GGTGTTACAG AATCAGGAAA TATTAAAAAT 
GTTTCTTCGA TATCACCAAG TCCAACAAAT AGCCAACTAC ATCCACAGCC TGCTCCTAGG 
CACAGCATTT CGCAACCAAC AATTACACCT ACAACTACTA CGAGTTCAAC GCCCACATCT 
TCTACAGAAC CTAGGTCAAC CTCACCCCAG GAAGATATAC AGGTACCCAA GCCTATCACC 
AATGAAGAAT TTCAGGCTAT GATTCCTGCT CATTTCCTTC GTCCTCCTAC TTCCTCACCA 
TCGCCAGATT CCCATCAAGG CCCTATCGTG ACAGTGACAA TAAAACAGCC TGATAATCTA 
CCTGGAGATG TTAATTTTCC TCCGGCTCCT ACAACACTTG GTAAAGTTAC TGAGACCATC 
ACAAAGAGCA CTCTCACCGA GACCGTCGTA ACTAGGGTGA CTGAAAATCA ATTGGTACCA 
CCAGTAATTA TTGAGGACGT GATTCTTGTA AAAGAGGGAT CTCTAGGATT TAGTATTATT 
GGTGGTACGG ATCATTCATG CACTCCATTT GGTGCAAAGG AACCTGGAAT ATTTATATCT 
CATGTCGTGC CTGGTGGTAT AGCTGCAAAA TCTGGTAAAT TAAGAATGGG TGATAGAATA 
CTAAAGGTAA ATGGTACTGA TGTAACAAAA GCTACTCATC AAGAGGCTGT GATGGAACTT 
TTGCGACCAG GAGATCAAAT AGTGCTTACT GTCCAACACG ATCCACTACC CGAAAATTAT 
CAGGAATTAG TTATTACAAA AGAACCAGGA GAGAAACTGG GCATGCATAT TAAAGGTGGA 
CTTCGAGGAC AAAAAGGTAA TCCACTTGAT CATACAGATG AAGGGGTTTT TATATCCAAA 
ATCAACTCAG GTGGTGCAGC TAAAAGAGAT GGAAGACTGA AAGTTGGAAT GAGACTATTA 
GAAGTTAATG GTACATCATT ATTAGGCGCA ACTCACCAAG AAGCAGTAAA TATATTGCGA 
TGTTCAGGAA ATACGATTAC TTTGGTGGTT TGTAAAGGAT ATGATAAGAG TGAAATTGAA 
CCAGTATTAC CAATATCCGA TGGAAGAGAT TCTAAGGAAT CTAGAACATC CAAAGAATTA 
AAGGATGCTA CAACAGAAGG TACTAAATCG TTATCACAAA GTATATCCAG TCTGGATCGC 
GATGATGAAG AAGCAGCAAC TCTTAGACAA GAACAGGAAA TGAAAGCTGA ACTTGTCGCA 
TGGGAACAAG AAGAAAGAGA ACGTGCTTTG CTTGAGCATA GAGAAAAATC TACTCCTGAA 
AAAGTATTAG ATGTAGTAAG AGCAGCTGAA TCATTAGTAA GCAAATCAAA TAGTCCGGTT 
GATATGGTCG TACCACCAAA GTCACCAGGT GGTACGAAAG ATCTCAAAAC AACCACTATT 
GTGATGAGCA AACATACTCT AGCACCTCAA AATACAAATT CACTGAGGAA AGAGAGAACT 
GGAACATCAG TGGACAATCC ATCGCCCAAG TCTCCGATCT CTACTTTAAC TCCATCGATG 
AATTTCGATC ACACTACTGC TGTCCAAAGC TTGCCCTCTC CAAAGAAAAA AAGTTCCATA 
CCAAAACGTA GTATTACATT TGCTGATCTT CCTCCCATTT CTAAAGAACC AATTAATTAT 
TCCATTTCGG CGAAAGAAGA TCCATCACCT TTGCATGATA GTAAATTTAC TTTCGATCCT 
CAAACTATTT CTTATAAGAA AGTCTATCAA AATCCTATCC AAGCTACTCA TTCTCGTTTC 
AAACTTCCTC CTACACCTTT AACTGCTCCT AAATCTATAG AAGATTATGG TACTTCAATT 
TCTTTTGGTA AACGACAGAA CGCACGCTCT GTTGATGGGA ATAGTCAGGT TGAGCTTTCT 
CCAACAACAT CACCAACACC ACCTCTAGTA AAGATGTCGG TTAGTGATAA AAAAAAATTG 
TTTGAAAGTG CCATGGAGGA ACATTTAAAA CCTTCCCCTA AACCAGAAAA AGTATTCAGT 
TTTCTAAGTC AGGATGAAGT TGAGAAAATG AAACAAGAAG AAGAAAAGAA GATTGCTACT 
CTAACGCGGG ATGAATTAAA GTCGTGGGCA CAACTTGATG AAAATGAAGG TTTGGAAGAT 
TTAGAAGAAA CATTAGAAGA TCAGGATAAT CGGCGACCTA ATAGCCGACT GAGTTCGCGA 
ACTTCGATCC CTCTTCTGCA AAATCTTCCA AGCAGTGTAA GGACAGCAAA AGCAGAACGC 
CGCTTGAAAG AAAGATTAAT ACAGGAGGGT ATAATTTCGG ATGAAGATGA AGAAAGTCAT 
TTAAGTCCTG CCGAACAGAG AGCGTTAAGA GCAGAAAAAC GTGCGGCATG GAGACAAGCA 
CGTTTGAAAT CTCTGGAACA AGATGCTCTT CAAGCACAGA TAGTAATTAA AAAAATGAGT 
GAAATGATGG ACACGAATAA AACAAGTTCA ATGCAGGATT CTACAGATAT TCCTATTACT 
CCTGTTTCTG AATCGGAAAA GACAACTGAT TTCACTACGT TACGGCCGTC TAGTGCAGAT 
TTTCCCAAAC TTGCTGTACG TAGCAAAGTG GGTCCCCCTA AAGAGATACG CGAATCCGAG 
AAAGTAGTGG ACGAGAAGGT CACTCGACGT ACTGAGGAAT ATGTGGATGA GGTGACGGGT 
GAACGACGTG TGCGAACAGT CGAATACGTC GAGAAACTCA TTGAACGACA GGTTGAAACT 
CTACGAGAAA AGATAATATC ATTGGAATTA AGTAATGCAG AAGATGAAAT AGAAAGTATT 
ACCGGTACAG GAGCAAGTGA TGCTGAAAGC GAAAGCGAAG AAATTACTGT ACAACCTCCT 
ACTATGACTA CTTTACAAGA AAGAAATGAA ACAATTATAT CTACAAATGA AGGTGCCGCA 
CCTAAAATAA GTGCAAATAC TGTTCTTGTT TCAAAAAAAA AGAAACGAAA ACGTTCGAAG 
AAAGGTCGTC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016921380.1
CDS	1476..7610
Translation	MFRCIPIFKGCNRQVEYIDKRHCSLPCVPDDILRYSRSLEELLLDANHIRDLPKNFFRLQKLRKLGLSDNEIHRLPPDIQNFENLVELDVSRNDIPDIPENIKNLRALQVADFSSNPIPRLPAGFVQLRNLTVLGLNDMSLTNLPPDFGSLEALQSLELRENLLKSLPESLSQLYKLERLDLGDNDIEVLPAHIGELPALQELWLDHNQLQHLPPEIGELKTLVCLDVSENRLEDLPEEIGGLEALTDLHLSQNVIEKLPDGLGELKKLTILKVDQNRLSTLNPNIGRCENLQELILTENFLLELPVSIGKLLNLNNLNVDRNSLQSLPTETGNLKQLGVLSLRDNKLQYLPIEVGQCTALHVLDVSGNRLQYLPYSLINLNLKAVWLSKNQAQPMLTFQTDVDEETGQEVLTCFLLPQLEIHPDDSGRLGMLAGMRNAVQDGEIRELGSSDDEGWQEKEASRTHSVKFTDEPPETDKETPFVRQNTPHPKELKAKAHKLFSKGKNDSRSASTDEQDVSQTFGLDKTETDVSTKPNDLTVDLIEQESLKHESVENEQKEIVPGVTDNIDVQSSNEPEIVSNQYIVESNTDVRTEGSISELKDRNDKEDDESENEETQRHVEFSIAEGTDYDGSGDSNRPNRLHRRDTPHHLKNKRIHTAVDKEKVASIIAQALMKKNDEISTSTSAPAETTENFDAQSQDAISDAEPTIEVREEQYEIHIERTTGGLGLSIAGGIGSTPFKGDDEGIFISRVTEGGPADLAGLKVEDKVLSVNGVSVVNVGHYDAVEVLKACGRVLVLVVQREVTRIVPPFEQQISSRKNSVCSSLSTSRAPSATSHVSSIGFSHNLENGDALSHDAIKCKKIPEPISSTKVGNEPMVSVLVHTTLIRDQNGLGFSIAGGEGSPPFKDNSDAIYISRITDGGVAQKDGKLLVGDKVISINGVEMRGAKHEQAVALLTGLERFVRLVVEREIPFSQANAATVVTPSEKSPRVIGAPKPYTGLYNANSYIANKPGYTGYRRSIDADRTLSPSPTSKTPPPMKIETTELPKMNGVTESGNIKNVSSISPSPTNSQLHPQPAPRHSISQPTITPTTTTSSTPTSSTEPRSTSPQEDIQVPKPITNEEFQAMIPAHFLRPPTSSPSPDSHQGPIVTVTIKQPDNLPGDVNFPPAPTTLGKVTETITKSTLTETVVTRVTENQLVPPVIIEDVILVKEGSLGFSIIGGTDHSCTPFGAKEPGIFISHVVPGGIAAKSGKLRMGDRILKVNGTDVTKATHQEAVMELLRPGDQIVLTVQHDPLPENYQELVITKEPGEKLGMHIKGGLRGQKGNPLDHTDEGVFISKINSGGAAKRDGRLKVGMRLLEVNGTSLLGATHQEAVNILRCSGNTITLVVCKGYDKSEIEPVLPISDGRDSKESRTSKELKDATTEGTKSLSQSISSLDRDDEEAATLRQEQEMKAELVAWEQEERERALLEHREKSTPEKVLDVVRAAESLVSKSNSPVDMVVPPKSPGGTKDLKTTTIVMSKHTLAPQNTNSLRKERTGTSVDNPSPKSPISTLTPSMNFDHTTAVQSLPSPKKKSSIPKRSITFADLPPISKEPINYSISAKEDPSPLHDSKFTFDPQTISYKKVYQNPIQATHSRFKLPPTPLTAPKSIEDYGTSISFGKRQNARSVDGNSQVELSPTTSPTPPLVKMSVSDKKKLFESAMEEHLKPSPKPEKVFSFLSQDEVEKMKQEEEKKIATLTRDELKSWAQLDENEGLEDLEETLEDQDNRRPNSRLSSRTSIPLLQNLPSSVRTAKAERRLKERLIQEGIISDEDEESHLSPAEQRALRAEKRAAWRQARLKSLEQDALQAQIVIKKMSEMMDTNKTSSMQDSTDIPITPVSESEKTTDFTTLRPSSADFPKLAVRSKVGPPKEIRESEKVVDEKVTRRTEEYVDEVTGERRVRTVEYVEKLIERQVETLREKIISLELSNAEDEIESITGTGASDAESESEEITVQPPTMTTLQERNETIISTNEGAAPKISANTVLVSKKKKRKRSKKGRH

Target ORF information:

RefSeq Version	XM_017065891.2
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana protein lap4 (LOC108003564), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017065891.2

ORF Insert Sequence:

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGC AGATATCATC GAGAAAAAAC 
TCAGTGTGCT CTAGTTTAAG TACTAGTAGA GCTCCAAGTG CAACATCTCA TGTTTCATCT 
ATAGGTTTTT CACATAATTT AGAAAATGGT GATGCTTTAT CTCATGATGC TATTAAGTGT 
AAGAAAATTC CTGAACCTAT ATCTTCAACA AAAGTAGGCA ATGAACCAAT GGTATCAGTT 
CTTGTTCACA CAACATTAAT ACGGGACCAA AATGGGCTTG GATTTAGTAT TGCTGGTGGA 
GAAGGTTCTC CACCATTCAA AGACAATAGT GATGCGATAT ATATTTCAAG AATAACAGAT 
GGCGGCGTAG CACAAAAAGA TGGTAAATTA TTAGTTGGAG ACAAAGTAAT ATCTATTAAT 
GGAGTTGAGA TGAGAGGAGC TAAGCATGAA CAAGCAGTTG CTTTATTAAC AGGTTTAGAA 
AGATTTGTTC GATTAGTAGT TGAACGCGAA ATACCATTTT CGCAAGCAAA TGCAGCTACG 
GTCGTAACAC CTTCAGAAAA ATCACCACGA GTGATCGGTG CACCTAAACC ATATACAGGA 
TTATATAATG CCAATAGTTA TATAGCAAAT AAACCGGGAT ATACTGGATA CAGACGTTCT 
ATAGATGCTG ATAGAACATT ATCTCCAAGC CCTACTTCGA AAACGCCTCC ACCTATGAAA 
ATTGAAACTA CTGAATTACC GAAAATGAAC GGTGTTACAG AATCAGGAAA TATTAAAAAT 
GTTTCTTCGA TATCACCAAG TCCAACAAAT AGCCAACTAC ATCCACAGCC TGCTCCTAGG 
CACAGCATTT CGCAACCAAC AATTACACCT ACAACTACTA CGAGTTCAAC GCCCACATCT 
TCTACAGAAC CTAGGTCAAC CTCACCCCAG GAAGATATAC AGGTACCCAA GCCTATCACC 
AATGAAGAAT TTCAGGCTAT GATTCCTGCT CATTTCCTTC GTCCTCCTAC TTCCTCACCA 
TCGCCAGATT CCCATCAAGG CCCTATCGTG ACAGTGACAA TAAAACAGCC TGATAATCTA 
CCTGGAGATG TTAATTTTCC TCCGGCTCCT ACAACACTTG GTAAAGTTAC TGAGACCATC 
ACAAAGAGCA CTCTCACCGA GACCGTCGTA ACTAGGGTGA CTGAAAATCA ATTGGTACCA 
CCAGTAATTA TTGAGGACGT GATTCTTGTA AAAGAGGGAT CTCTAGGATT TAGTATTATT 
GGTGGTACGG ATCATTCATG CACTCCATTT GGTGCAAAGG AACCTGGAAT ATTTATATCT 
CATGTCGTGC CTGGTGGTAT AGCTGCAAAA TCTGGTAAAT TAAGAATGGG TGATAGAATA 
CTAAAGGTAA ATGGTACTGA TGTAACAAAA GCTACTCATC AAGAGGCTGT GATGGAACTT 
TTGCGACCAG GAGATCAAAT AGTGCTTACT GTCCAACACG ATCCACTACC CGAAAATTAT 
CAGGAATTAG TTATTACAAA AGAACCAGGA GAGAAACTGG GCATGCATAT TAAAGGTGGA 
CTTCGAGGAC AAAAAGGTAA TCCACTTGAT CATACAGATG AAGGGGTTTT TATATCCAAA 
ATCAACTCAG GTGGTGCAGC TAAAAGAGAT GGAAGACTGA AAGTTGGAAT GAGACTATTA 
GAAGTTAATG GTACATCATT ATTAGGCGCA ACTCACCAAG AAGCAGTAAA TATATTGCGA 
TGTTCAGGAA ATACGATTAC TTTGGTGGTT TGTAAAGGAT ATGATAAGAG TGAAATTGAA 
CCAGTATTAC CAATATCCGA TGGAAGAGAT TCTAAGGAAT CTAGAACATC CAAAGAATTA 
AAGGATGCTA CAACAGAAGG TACTAAATCG TTATCACAAA GTATATCCAG TCTGGATCGC 
GATGATGAAG AAGCAGCAAC TCTTAGACAA GAACAGGAAA TGAAAGCTGA ACTTGTCGCA 
TGGGAACAAG AAGAAAGAGA ACGTGCTTTG CTTGAGCATA GAGAAAAATC TACTCCTGAA 
AAAGTATTAG ATGTAGTAAG AGCAGCTGAA TCATTAGTAA GCAAATCAAA TAGTCCGGTT 
GATATGGTCG TACCACCAAA GTCACCAGGT GGTACGAAAG ATCTCAAAAC AACCACTATT 
GTGATGAGCA AACATACTCT AGCACCTCAA AATACAAATT CACTGAGGAA AGAGAGAACT 
GGAACATCAG TGGACAATCC ATCGCCCAAG TCTCCGATCT CTACTTTAAC TCCATCGATG 
AATTTCGATC ACACTACTGC TGTCCAAAGC TTGCCCTCTC CAAAGAAAAA AAGTTCCATA 
CCAAAACGTA GTATTACATT TGCTGATCTT CCTCCCATTT CTAAAGAACC AATTAATTAT 
TCCATTTCGG CGAAAGAAGA TCCATCACCT TTGCATGATA GTAAATTTAC TTTCGATCCT 
CAAACTATTT CTTATAAGAA AGTCTATCAA AATCCTATCC AAGCTACTCA TTCTCGTTTC 
AAACTTCCTC CTACACCTTT AACTGCTCCT AAATCTATAG AAGATTATGG TACTTCAATT 
TCTTTTGGTA AACGACAGAA CGCACGCTCT GTTGATGGGA ATAGTCAGGT TGAGCTTTCT 
CCAACAACAT CACCAACACC ACCTCTAGTA AAGATGTCGG TTAGTGATAA AAAAAAATTG 
TTTGAAAGTG CCATGGAGGA ACATTTAAAA CCTTCCCCTA AACCAGAAAA AGTATTCAGT 
TTTCTAAGTC AGGATGAAGT TGAGAAAATG AAACAAGAAG AAGAAAAGAA GATTGCTACT 
CTAACGCGGG ATGAATTAAA GTCGTGGGCA CAACTTGATG AAAATGAAGG TTTGGAAGAT 
TTAGAAGAAA CATTAGAAGA TCAGGATAAT CGGCGACCTA ATAGCCGACT GAGTTCGCGA 
ACTTCGATCC CTCTTCTGCA AAATCTTCCA AGCAGTGTAA GGACAGCAAA AGCAGAACGC 
CGCTTGAAAG AAAGATTAAT ACAGGAGGGT ATAATTTCGG ATGAAGATGA AGAAAGTCAT 
TTAAGTCCTG CCGAACAGAG AGCGTTAAGA GCAGAAAAAC GTGCGGCATG GAGACAAGCA 
CGTTTGAAAT CTCTGGAACA AGATGCTCTT CAAGCACAGA TAGTAATTAA AAAAATGAGT 
GAAATGATGG ACACGAATAA AACAAGTTCA ATGCAGGATT CTACAGATAT TCCTATTACT 
CCTGTTTCTG AATCGGAAAA GACAACTGAT TTCACTACGT TACGGCCGTC TAGTGCAGAT 
TTTCCCAAAC TTGCTGTACG TAGCAAAGTG GGTCCCCCTA AAGAGATACG CGAATCCGAG 
AAAGTAGTGG ACGAGAAGGT CACTCGACGT ACTGAGGAAT ATGTGGATGA GGTGACGGGT 
GAACGACGTG TGCGAACAGT CGAATACGTC GAGAAACTCA TTGAACGACA GGTTGAAACT 
CTACGAGAAA AGATAATATC ATTGGAATTA AGTAATGCAG AAGATGAAAT AGAAAGTATT 
ACCGGTACAG GAGCAAGTGA TGCTGAAAGC GAAAGCGAAG AAATTACTGT ACAACCTCCT 
ACTATGACTA CTTTACAAGA AAGAAATGAA ACAATTATAT CTACAAATGA AGGTGCCGCA 
CCTAAAATAA GTGCAAATAC TGTTCTTGTT TCAAAAAAAA AGAAACGAAA ACGTTCGAAG 
AAAGGTCGTC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108003564 ( XM_017065891.1 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_016921380.1 Apis cerana protein lap4 (LOC108003564), transcript variant X5, mRNA.

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CloneID	OAp29725
Clone ID Related Accession (Same CDS sequence)	XM_017065891.2 , XM_017065891.1
Accession Version	XM_017065891.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-08
Organism	Apis cerana(Asiatic honeybee)
Product	protein lap4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016017567.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis cerana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGC AGATATCATC GAGAAAAAAC 
TCAGTGTGCT CTAGTTTAAG TACTAGTAGA GCTCCAAGTG CAACATCTCA TGTTTCATCT 
ATAGGTTTTT CACATAATTT AGAAAATGGT GATGCTTTAT CTCATGATGC TATTAAGTGT 
AAGAAAATTC CTGAACCTAT ATCTTCAACA AAAGTAGGCA ATGAACCAAT GGTATCAGTT 
CTTGTTCACA CAACATTAAT ACGGGACCAA AATGGGCTTG GATTTAGTAT TGCTGGTGGA 
GAAGGTTCTC CACCATTCAA AGACAATAGT GATGCGATAT ATATTTCAAG AATAACAGAT 
GGCGGCGTAG CACAAAAAGA TGGTAAATTA TTAGTTGGAG ACAAAGTAAT ATCTATTAAT 
GGAGTTGAGA TGAGAGGAGC TAAGCATGAA CAAGCAGTTG CTTTATTAAC AGGTTTAGAA 
AGATTTGTTC GATTAGTAGT TGAACGCGAA ATACCATTTT CGCAAGCAAA TGCAGCTACG 
GTCGTAACAC CTTCAGAAAA ATCACCACGA GTGATCGGTG CACCTAAACC ATATACAGGA 
TTATATAATG CCAATAGTTA TATAGCAAAT AAACCGGGAT ATACTGGATA CAGACGTTCT 
ATAGATGCTG ATAGAACATT ATCTCCAAGC CCTACTTCGA AAACGCCTCC ACCTATGAAA 
ATTGAAACTA CTGAATTACC GAAAATGAAC GGTGTTACAG AATCAGGAAA TATTAAAAAT 
GTTTCTTCGA TATCACCAAG TCCAACAAAT AGCCAACTAC ATCCACAGCC TGCTCCTAGG 
CACAGCATTT CGCAACCAAC AATTACACCT ACAACTACTA CGAGTTCAAC GCCCACATCT 
TCTACAGAAC CTAGGTCAAC CTCACCCCAG GAAGATATAC AGGTACCCAA GCCTATCACC 
AATGAAGAAT TTCAGGCTAT GATTCCTGCT CATTTCCTTC GTCCTCCTAC TTCCTCACCA 
TCGCCAGATT CCCATCAAGG CCCTATCGTG ACAGTGACAA TAAAACAGCC TGATAATCTA 
CCTGGAGATG TTAATTTTCC TCCGGCTCCT ACAACACTTG GTAAAGTTAC TGAGACCATC 
ACAAAGAGCA CTCTCACCGA GACCGTCGTA ACTAGGGTGA CTGAAAATCA ATTGGTACCA 
CCAGTAATTA TTGAGGACGT GATTCTTGTA AAAGAGGGAT CTCTAGGATT TAGTATTATT 
GGTGGTACGG ATCATTCATG CACTCCATTT GGTGCAAAGG AACCTGGAAT ATTTATATCT 
CATGTCGTGC CTGGTGGTAT AGCTGCAAAA TCTGGTAAAT TAAGAATGGG TGATAGAATA 
CTAAAGGTAA ATGGTACTGA TGTAACAAAA GCTACTCATC AAGAGGCTGT GATGGAACTT 
TTGCGACCAG GAGATCAAAT AGTGCTTACT GTCCAACACG ATCCACTACC CGAAAATTAT 
CAGGAATTAG TTATTACAAA AGAACCAGGA GAGAAACTGG GCATGCATAT TAAAGGTGGA 
CTTCGAGGAC AAAAAGGTAA TCCACTTGAT CATACAGATG AAGGGGTTTT TATATCCAAA 
ATCAACTCAG GTGGTGCAGC TAAAAGAGAT GGAAGACTGA AAGTTGGAAT GAGACTATTA 
GAAGTTAATG GTACATCATT ATTAGGCGCA ACTCACCAAG AAGCAGTAAA TATATTGCGA 
TGTTCAGGAA ATACGATTAC TTTGGTGGTT TGTAAAGGAT ATGATAAGAG TGAAATTGAA 
CCAGTATTAC CAATATCCGA TGGAAGAGAT TCTAAGGAAT CTAGAACATC CAAAGAATTA 
AAGGATGCTA CAACAGAAGG TACTAAATCG TTATCACAAA GTATATCCAG TCTGGATCGC 
GATGATGAAG AAGCAGCAAC TCTTAGACAA GAACAGGAAA TGAAAGCTGA ACTTGTCGCA 
TGGGAACAAG AAGAAAGAGA ACGTGCTTTG CTTGAGCATA GAGAAAAATC TACTCCTGAA 
AAAGTATTAG ATGTAGTAAG AGCAGCTGAA TCATTAGTAA GCAAATCAAA TAGTCCGGTT 
GATATGGTCG TACCACCAAA GTCACCAGGT GGTACGAAAG ATCTCAAAAC AACCACTATT 
GTGATGAGCA AACATACTCT AGCACCTCAA AATACAAATT CACTGAGGAA AGAGAGAACT 
GGAACATCAG TGGACAATCC ATCGCCCAAG TCTCCGATCT CTACTTTAAC TCCATCGATG 
AATTTCGATC ACACTACTGC TGTCCAAAGC TTGCCCTCTC CAAAGAAAAA AAGTTCCATA 
CCAAAACGTA GTATTACATT TGCTGATCTT CCTCCCATTT CTAAAGAACC AATTAATTAT 
TCCATTTCGG CGAAAGAAGA TCCATCACCT TTGCATGATA GTAAATTTAC TTTCGATCCT 
CAAACTATTT CTTATAAGAA AGTCTATCAA AATCCTATCC AAGCTACTCA TTCTCGTTTC 
AAACTTCCTC CTACACCTTT AACTGCTCCT AAATCTATAG AAGATTATGG TACTTCAATT 
TCTTTTGGTA AACGACAGAA CGCACGCTCT GTTGATGGGA ATAGTCAGGT TGAGCTTTCT 
CCAACAACAT CACCAACACC ACCTCTAGTA AAGATGTCGG TTAGTGATAA AAAAAAATTG 
TTTGAAAGTG CCATGGAGGA ACATTTAAAA CCTTCCCCTA AACCAGAAAA AGTATTCAGT 
TTTCTAAGTC AGGATGAAGT TGAGAAAATG AAACAAGAAG AAGAAAAGAA GATTGCTACT 
CTAACGCGGG ATGAATTAAA GTCGTGGGCA CAACTTGATG AAAATGAAGG TTTGGAAGAT 
TTAGAAGAAA CATTAGAAGA TCAGGATAAT CGGCGACCTA ATAGCCGACT GAGTTCGCGA 
ACTTCGATCC CTCTTCTGCA AAATCTTCCA AGCAGTGTAA GGACAGCAAA AGCAGAACGC 
CGCTTGAAAG AAAGATTAAT ACAGGAGGGT ATAATTTCGG ATGAAGATGA AGAAAGTCAT 
TTAAGTCCTG CCGAACAGAG AGCGTTAAGA GCAGAAAAAC GTGCGGCATG GAGACAAGCA 
CGTTTGAAAT CTCTGGAACA AGATGCTCTT CAAGCACAGA TAGTAATTAA AAAAATGAGT 
GAAATGATGG ACACGAATAA AACAAGTTCA ATGCAGGATT CTACAGATAT TCCTATTACT 
CCTGTTTCTG AATCGGAAAA GACAACTGAT TTCACTACGT TACGGCCGTC TAGTGCAGAT 
TTTCCCAAAC TTGCTGTACG TAGCAAAGTG GGTCCCCCTA AAGAGATACG CGAATCCGAG 
AAAGTAGTGG ACGAGAAGGT CACTCGACGT ACTGAGGAAT ATGTGGATGA GGTGACGGGT 
GAACGACGTG TGCGAACAGT CGAATACGTC GAGAAACTCA TTGAACGACA GGTTGAAACT 
CTACGAGAAA AGATAATATC ATTGGAATTA AGTAATGCAG AAGATGAAAT AGAAAGTATT 
ACCGGTACAG GAGCAAGTGA TGCTGAAAGC GAAAGCGAAG AAATTACTGT ACAACCTCCT 
ACTATGACTA CTTTACAAGA AAGAAATGAA ACAATTATAT CTACAAATGA AGGTGCCGCA 
CCTAAAATAA GTGCAAATAC TGTTCTTGTT TCAAAAAAAA AGAAACGAAA ACGTTCGAAG 
AAAGGTCGTC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016921380.1
CDS	233..6367
Translation	MFRCIPIFKGCNRQVEYIDKRHCSLPCVPDDILRYSRSLEELLLDANHIRDLPKNFFRLQKLRKLGLSDNEIHRLPPDIQNFENLVELDVSRNDIPDIPENIKNLRALQVADFSSNPIPRLPAGFVQLRNLTVLGLNDMSLTNLPPDFGSLEALQSLELRENLLKSLPESLSQLYKLERLDLGDNDIEVLPAHIGELPALQELWLDHNQLQHLPPEIGELKTLVCLDVSENRLEDLPEEIGGLEALTDLHLSQNVIEKLPDGLGELKKLTILKVDQNRLSTLNPNIGRCENLQELILTENFLLELPVSIGKLLNLNNLNVDRNSLQSLPTETGNLKQLGVLSLRDNKLQYLPIEVGQCTALHVLDVSGNRLQYLPYSLINLNLKAVWLSKNQAQPMLTFQTDVDEETGQEVLTCFLLPQLEIHPDDSGRLGMLAGMRNAVQDGEIRELGSSDDEGWQEKEASRTHSVKFTDEPPETDKETPFVRQNTPHPKELKAKAHKLFSKGKNDSRSASTDEQDVSQTFGLDKTETDVSTKPNDLTVDLIEQESLKHESVENEQKEIVPGVTDNIDVQSSNEPEIVSNQYIVESNTDVRTEGSISELKDRNDKEDDESENEETQRHVEFSIAEGTDYDGSGDSNRPNRLHRRDTPHHLKNKRIHTAVDKEKVASIIAQALMKKNDEISTSTSAPAETTENFDAQSQDAISDAEPTIEVREEQYEIHIERTTGGLGLSIAGGIGSTPFKGDDEGIFISRVTEGGPADLAGLKVEDKVLSVNGVSVVNVGHYDAVEVLKACGRVLVLVVQREVTRIVPPFEQQISSRKNSVCSSLSTSRAPSATSHVSSIGFSHNLENGDALSHDAIKCKKIPEPISSTKVGNEPMVSVLVHTTLIRDQNGLGFSIAGGEGSPPFKDNSDAIYISRITDGGVAQKDGKLLVGDKVISINGVEMRGAKHEQAVALLTGLERFVRLVVEREIPFSQANAATVVTPSEKSPRVIGAPKPYTGLYNANSYIANKPGYTGYRRSIDADRTLSPSPTSKTPPPMKIETTELPKMNGVTESGNIKNVSSISPSPTNSQLHPQPAPRHSISQPTITPTTTTSSTPTSSTEPRSTSPQEDIQVPKPITNEEFQAMIPAHFLRPPTSSPSPDSHQGPIVTVTIKQPDNLPGDVNFPPAPTTLGKVTETITKSTLTETVVTRVTENQLVPPVIIEDVILVKEGSLGFSIIGGTDHSCTPFGAKEPGIFISHVVPGGIAAKSGKLRMGDRILKVNGTDVTKATHQEAVMELLRPGDQIVLTVQHDPLPENYQELVITKEPGEKLGMHIKGGLRGQKGNPLDHTDEGVFISKINSGGAAKRDGRLKVGMRLLEVNGTSLLGATHQEAVNILRCSGNTITLVVCKGYDKSEIEPVLPISDGRDSKESRTSKELKDATTEGTKSLSQSISSLDRDDEEAATLRQEQEMKAELVAWEQEERERALLEHREKSTPEKVLDVVRAAESLVSKSNSPVDMVVPPKSPGGTKDLKTTTIVMSKHTLAPQNTNSLRKERTGTSVDNPSPKSPISTLTPSMNFDHTTAVQSLPSPKKKSSIPKRSITFADLPPISKEPINYSISAKEDPSPLHDSKFTFDPQTISYKKVYQNPIQATHSRFKLPPTPLTAPKSIEDYGTSISFGKRQNARSVDGNSQVELSPTTSPTPPLVKMSVSDKKKLFESAMEEHLKPSPKPEKVFSFLSQDEVEKMKQEEEKKIATLTRDELKSWAQLDENEGLEDLEETLEDQDNRRPNSRLSSRTSIPLLQNLPSSVRTAKAERRLKERLIQEGIISDEDEESHLSPAEQRALRAEKRAAWRQARLKSLEQDALQAQIVIKKMSEMMDTNKTSSMQDSTDIPITPVSESEKTTDFTTLRPSSADFPKLAVRSKVGPPKEIRESEKVVDEKVTRRTEEYVDEVTGERRVRTVEYVEKLIERQVETLREKIISLELSNAEDEIESITGTGASDAESESEEITVQPPTMTTLQERNETIISTNEGAAPKISANTVLVSKKKKRKRSKKGRH

Target ORF information:

RefSeq Version	XM_017065891.1
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana protein lap4 (LOC108003564), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017065891.1

ORF Insert Sequence:

ATGTTCCGCT GCATTCCAAT TTTTAAAGGA TGTAACAGGC AGGTGGAATA CATTGACAAG 
CGTCATTGCT CTCTGCCGTG CGTCCCCGAT GATATTTTAC GATACTCGAG AAGTTTGGAG 
GAGCTCCTAC TGGACGCGAA TCATATTCGT GATCTACCGA AGAATTTTTT TCGGCTACAG 
AAGTTACGAA AGCTCGGCTT GAGTGACAAT GAAATTCACC GTTTGCCTCC AGATATTCAG 
AACTTCGAAA ATCTCGTGGA ACTGGATGTA TCCAGGAATG ATATACCAGA CATTCCTGAG 
AACATCAAGA ATTTACGGGC ACTTCAGGTG GCGGATTTCA GTAGTAATCC TATTCCAAGG 
CTTCCAGCAG GGTTTGTGCA ATTAAGAAAT TTAACTGTTT TGGGACTTAA TGATATGTCT 
CTTACGAATC TACCGCCTGA TTTCGGAAGC TTAGAGGCGT TACAATCATT GGAGTTAAGG 
GAAAATTTGC TCAAGTCCTT GCCAGAATCG CTATCCCAAC TTTATAAATT GGAGCGGCTA 
GATCTTGGTG ACAATGACAT TGAAGTTTTA CCAGCACATA TAGGAGAATT ACCAGCATTA 
CAAGAATTAT GGCTGGATCA TAATCAGTTA CAACATTTAC CACCAGAAAT AGGAGAATTG 
AAAACGTTAG TGTGTCTGGA TGTATCTGAA AATCGTTTGG AAGATCTCCC AGAAGAAATA 
GGAGGTCTAG AGGCTTTGAC GGATCTTCAT TTATCGCAAA ATGTTATTGA AAAATTACCT 
GATGGGCTTG GAGAATTAAA GAAACTTACT ATTCTTAAAG TCGATCAAAA TAGGCTTTCT 
ACTCTAAATC CAAATATAGG CAGGTGTGAA AATTTACAAG AATTAATTCT TACGGAAAAT 
TTTCTCTTAG AACTGCCTGT ATCTATAGGA AAACTTCTTA ATCTAAATAA TTTGAATGTA 
GATAGGAATA GTTTGCAATC ACTTCCTACG GAAACGGGAA ATTTAAAACA ATTGGGTGTA 
CTGTCCTTAA GAGATAATAA ATTACAATAT CTTCCAATCG AAGTTGGACA ATGTACTGCT 
CTCCATGTAT TGGATGTGTC TGGCAATAGA TTACAATACT TGCCATATTC CTTAATCAAT 
TTGAACTTGA AGGCAGTATG GTTAAGTAAA AATCAAGCAC AACCAATGCT TACTTTTCAG 
ACAGATGTTG ATGAAGAAAC TGGACAAGAA GTATTAACCT GTTTTCTTTT ACCACAATTA 
GAAATTCATC CAGATGATTC AGGAAGACTT GGTATGCTTG CTGGAATGAG AAATGCTGTT 
CAAGATGGTG AAATACGTGA ACTTGGTAGT AGTGATGATG AAGGATGGCA AGAAAAAGAA 
GCATCAAGAA CGCATTCTGT CAAGTTTACA GATGAACCTC CAGAAACTGA TAAGGAGACA 
CCTTTTGTAC GACAAAATAC ACCTCATCCC AAAGAATTAA AAGCAAAAGC TCATAAATTA 
TTTAGTAAAG GAAAAAATGA CAGTCGTTCA GCATCTACAG ATGAGCAAGA TGTTTCTCAA 
ACATTTGGTT TGGACAAAAC TGAAACTGAT GTTTCAACAA AACCTAACGA TTTGACAGTT 
GATTTGATAG AACAAGAATC ATTAAAACAT GAATCTGTAG AAAATGAACA AAAAGAAATT 
GTACCTGGAG TAACTGATAA TATAGATGTT CAATCTAGTA ATGAACCAGA AATAGTTTCT 
AATCAATATA TAGTAGAATC TAATACTGAC GTAAGAACTG AAGGTTCAAT ATCTGAGTTA 
AAGGATAGAA ATGATAAGGA GGATGATGAA TCTGAAAATG AAGAAACACA AAGACATGTA 
GAATTTTCTA TAGCTGAAGG TACAGATTAT GATGGTAGTG GTGATTCAAA TAGACCAAAT 
AGACTTCATC GTAGAGATAC TCCACATCAT TTGAAAAACA AGCGAATTCA TACAGCAGTG 
GATAAAGAAA AAGTAGCATC CATTATTGCA CAAGCACTCA TGAAAAAAAA TGATGAAATT 
TCCACATCCA CATCAGCACC TGCAGAAACT ACAGAAAATT TTGATGCTCA AAGTCAAGAT 
GCGATTTCAG ATGCTGAGCC GACAATAGAA GTACGAGAAG AACAATACGA AATTCACATC 
GAAAGAACAA CAGGTGGTCT AGGCTTATCA ATAGCTGGTG GTATTGGTTC AACCCCTTTT 
AAAGGCGATG ATGAAGGCAT TTTTATTTCC AGAGTTACTG AAGGTGGACC TGCAGATTTA 
GCAGGTTTAA AAGTGGAAGA TAAAGTATTA TCTGTTAATG GTGTATCAGT AGTAAATGTA 
GGTCATTATG ATGCTGTAGA AGTTTTGAAA GCTTGTGGAC GAGTTCTTGT ATTAGTAGTT 
CAAAGAGAAG TTACAAGGAT AGTGCCTCCA TTTGAACAGC AGATATCATC GAGAAAAAAC 
TCAGTGTGCT CTAGTTTAAG TACTAGTAGA GCTCCAAGTG CAACATCTCA TGTTTCATCT 
ATAGGTTTTT CACATAATTT AGAAAATGGT GATGCTTTAT CTCATGATGC TATTAAGTGT 
AAGAAAATTC CTGAACCTAT ATCTTCAACA AAAGTAGGCA ATGAACCAAT GGTATCAGTT 
CTTGTTCACA CAACATTAAT ACGGGACCAA AATGGGCTTG GATTTAGTAT TGCTGGTGGA 
GAAGGTTCTC CACCATTCAA AGACAATAGT GATGCGATAT ATATTTCAAG AATAACAGAT 
GGCGGCGTAG CACAAAAAGA TGGTAAATTA TTAGTTGGAG ACAAAGTAAT ATCTATTAAT 
GGAGTTGAGA TGAGAGGAGC TAAGCATGAA CAAGCAGTTG CTTTATTAAC AGGTTTAGAA 
AGATTTGTTC GATTAGTAGT TGAACGCGAA ATACCATTTT CGCAAGCAAA TGCAGCTACG 
GTCGTAACAC CTTCAGAAAA ATCACCACGA GTGATCGGTG CACCTAAACC ATATACAGGA 
TTATATAATG CCAATAGTTA TATAGCAAAT AAACCGGGAT ATACTGGATA CAGACGTTCT 
ATAGATGCTG ATAGAACATT ATCTCCAAGC CCTACTTCGA AAACGCCTCC ACCTATGAAA 
ATTGAAACTA CTGAATTACC GAAAATGAAC GGTGTTACAG AATCAGGAAA TATTAAAAAT 
GTTTCTTCGA TATCACCAAG TCCAACAAAT AGCCAACTAC ATCCACAGCC TGCTCCTAGG 
CACAGCATTT CGCAACCAAC AATTACACCT ACAACTACTA CGAGTTCAAC GCCCACATCT 
TCTACAGAAC CTAGGTCAAC CTCACCCCAG GAAGATATAC AGGTACCCAA GCCTATCACC 
AATGAAGAAT TTCAGGCTAT GATTCCTGCT CATTTCCTTC GTCCTCCTAC TTCCTCACCA 
TCGCCAGATT CCCATCAAGG CCCTATCGTG ACAGTGACAA TAAAACAGCC TGATAATCTA 
CCTGGAGATG TTAATTTTCC TCCGGCTCCT ACAACACTTG GTAAAGTTAC TGAGACCATC 
ACAAAGAGCA CTCTCACCGA GACCGTCGTA ACTAGGGTGA CTGAAAATCA ATTGGTACCA 
CCAGTAATTA TTGAGGACGT GATTCTTGTA AAAGAGGGAT CTCTAGGATT TAGTATTATT 
GGTGGTACGG ATCATTCATG CACTCCATTT GGTGCAAAGG AACCTGGAAT ATTTATATCT 
CATGTCGTGC CTGGTGGTAT AGCTGCAAAA TCTGGTAAAT TAAGAATGGG TGATAGAATA 
CTAAAGGTAA ATGGTACTGA TGTAACAAAA GCTACTCATC AAGAGGCTGT GATGGAACTT 
TTGCGACCAG GAGATCAAAT AGTGCTTACT GTCCAACACG ATCCACTACC CGAAAATTAT 
CAGGAATTAG TTATTACAAA AGAACCAGGA GAGAAACTGG GCATGCATAT TAAAGGTGGA 
CTTCGAGGAC AAAAAGGTAA TCCACTTGAT CATACAGATG AAGGGGTTTT TATATCCAAA 
ATCAACTCAG GTGGTGCAGC TAAAAGAGAT GGAAGACTGA AAGTTGGAAT GAGACTATTA 
GAAGTTAATG GTACATCATT ATTAGGCGCA ACTCACCAAG AAGCAGTAAA TATATTGCGA 
TGTTCAGGAA ATACGATTAC TTTGGTGGTT TGTAAAGGAT ATGATAAGAG TGAAATTGAA 
CCAGTATTAC CAATATCCGA TGGAAGAGAT TCTAAGGAAT CTAGAACATC CAAAGAATTA 
AAGGATGCTA CAACAGAAGG TACTAAATCG TTATCACAAA GTATATCCAG TCTGGATCGC 
GATGATGAAG AAGCAGCAAC TCTTAGACAA GAACAGGAAA TGAAAGCTGA ACTTGTCGCA 
TGGGAACAAG AAGAAAGAGA ACGTGCTTTG CTTGAGCATA GAGAAAAATC TACTCCTGAA 
AAAGTATTAG ATGTAGTAAG AGCAGCTGAA TCATTAGTAA GCAAATCAAA TAGTCCGGTT 
GATATGGTCG TACCACCAAA GTCACCAGGT GGTACGAAAG ATCTCAAAAC AACCACTATT 
GTGATGAGCA AACATACTCT AGCACCTCAA AATACAAATT CACTGAGGAA AGAGAGAACT 
GGAACATCAG TGGACAATCC ATCGCCCAAG TCTCCGATCT CTACTTTAAC TCCATCGATG 
AATTTCGATC ACACTACTGC TGTCCAAAGC TTGCCCTCTC CAAAGAAAAA AAGTTCCATA 
CCAAAACGTA GTATTACATT TGCTGATCTT CCTCCCATTT CTAAAGAACC AATTAATTAT 
TCCATTTCGG CGAAAGAAGA TCCATCACCT TTGCATGATA GTAAATTTAC TTTCGATCCT 
CAAACTATTT CTTATAAGAA AGTCTATCAA AATCCTATCC AAGCTACTCA TTCTCGTTTC 
AAACTTCCTC CTACACCTTT AACTGCTCCT AAATCTATAG AAGATTATGG TACTTCAATT 
TCTTTTGGTA AACGACAGAA CGCACGCTCT GTTGATGGGA ATAGTCAGGT TGAGCTTTCT 
CCAACAACAT CACCAACACC ACCTCTAGTA AAGATGTCGG TTAGTGATAA AAAAAAATTG 
TTTGAAAGTG CCATGGAGGA ACATTTAAAA CCTTCCCCTA AACCAGAAAA AGTATTCAGT 
TTTCTAAGTC AGGATGAAGT TGAGAAAATG AAACAAGAAG AAGAAAAGAA GATTGCTACT 
CTAACGCGGG ATGAATTAAA GTCGTGGGCA CAACTTGATG AAAATGAAGG TTTGGAAGAT 
TTAGAAGAAA CATTAGAAGA TCAGGATAAT CGGCGACCTA ATAGCCGACT GAGTTCGCGA 
ACTTCGATCC CTCTTCTGCA AAATCTTCCA AGCAGTGTAA GGACAGCAAA AGCAGAACGC 
CGCTTGAAAG AAAGATTAAT ACAGGAGGGT ATAATTTCGG ATGAAGATGA AGAAAGTCAT 
TTAAGTCCTG CCGAACAGAG AGCGTTAAGA GCAGAAAAAC GTGCGGCATG GAGACAAGCA 
CGTTTGAAAT CTCTGGAACA AGATGCTCTT CAAGCACAGA TAGTAATTAA AAAAATGAGT 
GAAATGATGG ACACGAATAA AACAAGTTCA ATGCAGGATT CTACAGATAT TCCTATTACT 
CCTGTTTCTG AATCGGAAAA GACAACTGAT TTCACTACGT TACGGCCGTC TAGTGCAGAT 
TTTCCCAAAC TTGCTGTACG TAGCAAAGTG GGTCCCCCTA AAGAGATACG CGAATCCGAG 
AAAGTAGTGG ACGAGAAGGT CACTCGACGT ACTGAGGAAT ATGTGGATGA GGTGACGGGT 
GAACGACGTG TGCGAACAGT CGAATACGTC GAGAAACTCA TTGAACGACA GGTTGAAACT 
CTACGAGAAA AGATAATATC ATTGGAATTA AGTAATGCAG AAGATGAAAT AGAAAGTATT 
ACCGGTACAG GAGCAAGTGA TGCTGAAAGC GAAAGCGAAG AAATTACTGT ACAACCTCCT 
ACTATGACTA CTTTACAAGA AAGAAATGAA ACAATTATAT CTACAAATGA AGGTGCCGCA 
CCTAAAATAA GTGCAAATAC TGTTCTTGTT TCAAAAAAAA AGAAACGAAA ACGTTCGAAG 
AAAGGTCGTC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC108003564 cDNA ORF clone, Apis cerana(Asiatic honeybee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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