LOC107996013 cDNA ORF clone, Apis cerana(Asiatic honeybee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC107996013
Entrez Gene ID	107996013
Full Name	valine--tRNA ligase
Gene Type	protein-coding
Organism	Apis cerana(Asiatic honeybee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017053864.2	XP_016909353.1	valine--tRNA ligase isoform X1
XM_017053864.1	XP_016909353.1	valine--tRNA ligase
XM_028665766.1	XP_028521567.1	valine--tRNA ligase isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC107996013 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC107996013 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAp175680	XM_028665766.1 Latest version!	Apis cerana valine--tRNA ligase (LOC107996013), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OAp34934	XM_017053864.2 Latest version!	Apis cerana valine--tRNA ligase (LOC107996013), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OAp34934	XM_017053864.1	Apis cerana valine--tRNA ligase (LOC107996013), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC107996013 ( XM_028665766.1 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_028521567.1 Apis cerana valine--tRNA ligase (LOC107996013), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAp175680
Clone ID Related Accession (Same CDS sequence)	XM_028665766.1
Accession Version	XM_028665766.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-16
Organism	Apis cerana(Asiatic honeybee)
Product	valine--tRNA ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016019011.1) and transcript sequence (JR047734.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis cerana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1170 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAAAGGAC TTACAACATT ATGGAATCCA GGTTGTGATC ATGCAGGTAT TGCTACTCAG 
GTAGTAGTTG AAAAAAAACT TTGGAAAGAA GAACAAAAAA CACGCCATGA TGTAGGAAGA 
GAAAATTTTA TAGAAAAAGT TTGGAAATGG AAAGAAGAAA AAGGAGACAG AATTTACCTG 
CAATTAAGAA AATTAGGTGG ATCATTTGAT TGGGATCGAG CTTGTTTTAC AATGGATCCA 
AAATTGTGTC GTGCTGTTTC AGAAGCATTT ATAAGATTGC ATGATGAGAA TATAATATAT 
AGAAGTAATA GATTAGTCAA TTGGTCCTGC ACATTAAAAA GTGCTATTTC TGATATAGAA 
GTTGATAAAC TCGAATTAAC TGGTCGAACA TTACTTTCAA TTCCTGGATA TCAAGAAAAA 
ATAGAATTTG GAATTTTAGT ATTATTTGCT TATGAAGTGA TAGACTTTGA AAAAAAAATA 
ATAGTTGCTA CTACTCGCAT TGAAACAATG CTTGGAGATA CAGCTATTGC TGTTCACCCA 
AAAGATAGTA GATATATTGA TTTCATTGGA AGATATGTAC AACATCCATT TTGTGATAGA 
AAATTACCAA TTATAGCTGA TGAATTTGTA GAAATGGAAT TCGGAACAGG TGCTGTAAAA 
ATTACACCAG CTCATGATCC TAATGATTAC GAAGTTGGAA AGAGACATAA TTTACCATTT 
ATAACTATTT TTGATGACAC TGGAAATATT ATTGGAGATT ATAGACAATT TACGGGTATG 
AAACGATTCC ATGTTCGAGC AGCTATAATA AAAGAATTAA TTGCAAAAAA TTTATTCATT 
GAAATTAAAG AGAATCCTAT GGTTGTGCCG ATATGTAGTC GATCTAAGGA TGTTGTTGAA 
CCATTAATGA AACCTCAATG GTATGTAAAA TGTAGCGAAA TGGCCTCAAA AGCAAAAGAT 
GCAGTAAAAT CCGGCGAATT AAAAATTATT CCGTCTCGAT ATAAAAAAAT TTGGTATCAT 
TGGATGGAAA ATATCACAGA TTGGTGTATA TCTCGGCAAT TATGGTGGGG TCATCGTATT 
CCTGCTTATT ATGTTAAAAT AATTAATCCA AATATAAAAG TCAAGCTAGA TGATGATTAT 
TGGGTAAGTG CACATTCAGA AAATGAAGCT AAAAAAAAGG CTGCAAAACA GTTAGGTATA 
TCTGTAGATA ATATTATTGT TGAACAAGAT CCTGATGTAT TAGATACATG GTTCTCTTCG 
GGTCTGTTTC CATTTTCGAT ATTCGGATGG CCAGATAAAA CTGAGGAATT TGAAGCATTT 
TATCCTGGTA CATTATTAGA AACTGGACAT GATATTTTAT TTTTCTGGGT AGCAAGAATG 
GTCTTTTTAG GTCAAAAGTT AATAGGAAAA TTACCATTTA AAGAGGTTTA TTTACATGCT 
ATGGTACGAG ATGCTCATGG CAGAAAAATG AGTAAATCAT TAGGAAATGT TATAGATCCT 
ATGGATGTAA TTAATGGGAT ATCATTAGAA GATCTTCATA AACAATTAAT AAATTCAAAT 
TTGGATCCAA AAGAATTTAA ATATGCTATT GAGGGACAAA AACGTGATTT TCCACAAGGG 
ATTCCCGAAT GTGGAACTGA TGCTTTAAGA TTTGCACTTT GCGCTTATAC GATGCAAGGC 
CGCGATATTA ATCTTGACAT TCTTCGCGTG CAGGGATATC GATTTTTCTG TAATAAAATA 
TGGAATGCGA CGAAATTCGC TCTTATGTAT TTAAATTCTA ATTTTGATGA AGATGTTCAA 
TCGACAAATG ATATTGTAGT AGGTGACAAT GATTGGTATC AAAATACTTT AAAAGAAAAA 
TGTGTGCAAG AAGCATTAAA TAATTATTTG GCAGATTATT CTTACTTAGA TGGATATAAA 
CCTTCACAAA TTGATACAAA AGTTTATCAA ACCTTTATCG AACTTGATAA TGATTTTACT 
AACTATCCTC ATTTACATCG TTGGTATAAA CATATGGCAT CTTATAATAA TCAAGAAAGA 
TCTATGTTTC ATGCAGAGAA AGGTAAAATA TTATCTCAAT GTGGTTGTAA AATACATGAT 
CACAATAATA AGAAAAAACA GTTACATTGT GAGTCAAATA TAGATTCTTG GATGCTTTCT 
CGCGTAAGTT ATGCAATAAA GACATGTAAT GAAGCAATGA CGCAATATGA TTTTCCAACC 
GCCACTACTG CTTGCTATAA TCTTTGGCTA TATGATTTAT GCGATATTTA TTTGGAGTAT 
TTGAAACCAG TATTTCAAAG CGATGATGTT AAAAGAAAAT ATGCAGCAAA GAAAACTTTA 
TTTAAAACAT TAGATATAGG ATTAAGATTG TTAAGTCCTT TTATGCCATT TATAACAGAA 
GAACTTTATC AACGTTTGCC TCATAAAAAA CAATTTTATC CTAGTATTTG TGTTAGTCCA 
TATCCAGAAG TTACAGAGTA TTCTTGGAGG AATGAAGAAA TAGAAAAAGA TGTTGATTTT 
ATCAATAAAG TAATAAAAAA TATTCGATCA ACGCGTGCAA CATATAATTT ACCAAATAAA 
ATCAAGACTG AAGCATTTCT TGTATGTAAT TCTAATAGCT TAAAAGAAAA GCTTTTAGAA 
TATCAATTGT TGATAGAAAC TTTATCTTAC TCTACACTCA ATACAACAGA ACCACCAACA 
GGATGTGCTA TCATTACTAT CACGGATAAA ATTCAAGTTC ATCTATTGCT AAAAGGTTTG 
ATCGATTCAA AGAAGGAGAT AGAGAAACTT AGCAAAAAAC AGGAGCAATT AATAGATGTT 
ATTCATAAAA TTAAGCAAAC AATGGAAATT CCTGATTATA ATGTTAAAGT ACCTTTGGAT 
GTACAAGATA ATAACACAGA GAAATTAACA AACAATGAAG GAGAATTGCA ACGAATCATT 
GATGCATTGT CGGTATTACG GGCAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028521567.1
CDS	202..3171
Translation	MKGLTTLWNPGCDHAGIATQVVVEKKLWKEEQKTRHDVGRENFIEKVWKWKEEKGDRIYLQLRKLGGSFDWDRACFTMDPKLCRAVSEAFIRLHDENIIYRSNRLVNWSCTLKSAISDIEVDKLELTGRTLLSIPGYQEKIEFGILVLFAYEVIDFEKKIIVATTRIETMLGDTAIAVHPKDSRYIDFIGRYVQHPFCDRKLPIIADEFVEMEFGTGAVKITPAHDPNDYEVGKRHNLPFITIFDDTGNIIGDYRQFTGMKRFHVRAAIIKELIAKNLFIEIKENPMVVPICSRSKDVVEPLMKPQWYVKCSEMASKAKDAVKSGELKIIPSRYKKIWYHWMENITDWCISRQLWWGHRIPAYYVKIINPNIKVKLDDDYWVSAHSENEAKKKAAKQLGISVDNIIVEQDPDVLDTWFSSGLFPFSIFGWPDKTEEFEAFYPGTLLETGHDILFFWVARMVFLGQKLIGKLPFKEVYLHAMVRDAHGRKMSKSLGNVIDPMDVINGISLEDLHKQLINSNLDPKEFKYAIEGQKRDFPQGIPECGTDALRFALCAYTMQGRDINLDILRVQGYRFFCNKIWNATKFALMYLNSNFDEDVQSTNDIVVGDNDWYQNTLKEKCVQEALNNYLADYSYLDGYKPSQIDTKVYQTFIELDNDFTNYPHLHRWYKHMASYNNQERSMFHAEKGKILSQCGCKIHDHNNKKKQLHCESNIDSWMLSRVSYAIKTCNEAMTQYDFPTATTACYNLWLYDLCDIYLEYLKPVFQSDDVKRKYAAKKTLFKTLDIGLRLLSPFMPFITEELYQRLPHKKQFYPSICVSPYPEVTEYSWRNEEIEKDVDFINKVIKNIRSTRATYNLPNKIKTEAFLVCNSNSLKEKLLEYQLLIETLSYSTLNTTEPPTGCAIITITDKIQVHLLLKGLIDSKKEIEKLSKKQEQLIDVIHKIKQTMEIPDYNVKVPLDVQDNNTEKLTNNEGELQRIIDALSVLRAI

Target ORF information:

RefSeq Version	XM_028665766.1
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana valine--tRNA ligase (LOC107996013), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028665766.1

ORF Insert Sequence:

ATGAAAGGAC TTACAACATT ATGGAATCCA GGTTGTGATC ATGCAGGTAT TGCTACTCAG 
GTAGTAGTTG AAAAAAAACT TTGGAAAGAA GAACAAAAAA CACGCCATGA TGTAGGAAGA 
GAAAATTTTA TAGAAAAAGT TTGGAAATGG AAAGAAGAAA AAGGAGACAG AATTTACCTG 
CAATTAAGAA AATTAGGTGG ATCATTTGAT TGGGATCGAG CTTGTTTTAC AATGGATCCA 
AAATTGTGTC GTGCTGTTTC AGAAGCATTT ATAAGATTGC ATGATGAGAA TATAATATAT 
AGAAGTAATA GATTAGTCAA TTGGTCCTGC ACATTAAAAA GTGCTATTTC TGATATAGAA 
GTTGATAAAC TCGAATTAAC TGGTCGAACA TTACTTTCAA TTCCTGGATA TCAAGAAAAA 
ATAGAATTTG GAATTTTAGT ATTATTTGCT TATGAAGTGA TAGACTTTGA AAAAAAAATA 
ATAGTTGCTA CTACTCGCAT TGAAACAATG CTTGGAGATA CAGCTATTGC TGTTCACCCA 
AAAGATAGTA GATATATTGA TTTCATTGGA AGATATGTAC AACATCCATT TTGTGATAGA 
AAATTACCAA TTATAGCTGA TGAATTTGTA GAAATGGAAT TCGGAACAGG TGCTGTAAAA 
ATTACACCAG CTCATGATCC TAATGATTAC GAAGTTGGAA AGAGACATAA TTTACCATTT 
ATAACTATTT TTGATGACAC TGGAAATATT ATTGGAGATT ATAGACAATT TACGGGTATG 
AAACGATTCC ATGTTCGAGC AGCTATAATA AAAGAATTAA TTGCAAAAAA TTTATTCATT 
GAAATTAAAG AGAATCCTAT GGTTGTGCCG ATATGTAGTC GATCTAAGGA TGTTGTTGAA 
CCATTAATGA AACCTCAATG GTATGTAAAA TGTAGCGAAA TGGCCTCAAA AGCAAAAGAT 
GCAGTAAAAT CCGGCGAATT AAAAATTATT CCGTCTCGAT ATAAAAAAAT TTGGTATCAT 
TGGATGGAAA ATATCACAGA TTGGTGTATA TCTCGGCAAT TATGGTGGGG TCATCGTATT 
CCTGCTTATT ATGTTAAAAT AATTAATCCA AATATAAAAG TCAAGCTAGA TGATGATTAT 
TGGGTAAGTG CACATTCAGA AAATGAAGCT AAAAAAAAGG CTGCAAAACA GTTAGGTATA 
TCTGTAGATA ATATTATTGT TGAACAAGAT CCTGATGTAT TAGATACATG GTTCTCTTCG 
GGTCTGTTTC CATTTTCGAT ATTCGGATGG CCAGATAAAA CTGAGGAATT TGAAGCATTT 
TATCCTGGTA CATTATTAGA AACTGGACAT GATATTTTAT TTTTCTGGGT AGCAAGAATG 
GTCTTTTTAG GTCAAAAGTT AATAGGAAAA TTACCATTTA AAGAGGTTTA TTTACATGCT 
ATGGTACGAG ATGCTCATGG CAGAAAAATG AGTAAATCAT TAGGAAATGT TATAGATCCT 
ATGGATGTAA TTAATGGGAT ATCATTAGAA GATCTTCATA AACAATTAAT AAATTCAAAT 
TTGGATCCAA AAGAATTTAA ATATGCTATT GAGGGACAAA AACGTGATTT TCCACAAGGG 
ATTCCCGAAT GTGGAACTGA TGCTTTAAGA TTTGCACTTT GCGCTTATAC GATGCAAGGC 
CGCGATATTA ATCTTGACAT TCTTCGCGTG CAGGGATATC GATTTTTCTG TAATAAAATA 
TGGAATGCGA CGAAATTCGC TCTTATGTAT TTAAATTCTA ATTTTGATGA AGATGTTCAA 
TCGACAAATG ATATTGTAGT AGGTGACAAT GATTGGTATC AAAATACTTT AAAAGAAAAA 
TGTGTGCAAG AAGCATTAAA TAATTATTTG GCAGATTATT CTTACTTAGA TGGATATAAA 
CCTTCACAAA TTGATACAAA AGTTTATCAA ACCTTTATCG AACTTGATAA TGATTTTACT 
AACTATCCTC ATTTACATCG TTGGTATAAA CATATGGCAT CTTATAATAA TCAAGAAAGA 
TCTATGTTTC ATGCAGAGAA AGGTAAAATA TTATCTCAAT GTGGTTGTAA AATACATGAT 
CACAATAATA AGAAAAAACA GTTACATTGT GAGTCAAATA TAGATTCTTG GATGCTTTCT 
CGCGTAAGTT ATGCAATAAA GACATGTAAT GAAGCAATGA CGCAATATGA TTTTCCAACC 
GCCACTACTG CTTGCTATAA TCTTTGGCTA TATGATTTAT GCGATATTTA TTTGGAGTAT 
TTGAAACCAG TATTTCAAAG CGATGATGTT AAAAGAAAAT ATGCAGCAAA GAAAACTTTA 
TTTAAAACAT TAGATATAGG ATTAAGATTG TTAAGTCCTT TTATGCCATT TATAACAGAA 
GAACTTTATC AACGTTTGCC TCATAAAAAA CAATTTTATC CTAGTATTTG TGTTAGTCCA 
TATCCAGAAG TTACAGAGTA TTCTTGGAGG AATGAAGAAA TAGAAAAAGA TGTTGATTTT 
ATCAATAAAG TAATAAAAAA TATTCGATCA ACGCGTGCAA CATATAATTT ACCAAATAAA 
ATCAAGACTG AAGCATTTCT TGTATGTAAT TCTAATAGCT TAAAAGAAAA GCTTTTAGAA 
TATCAATTGT TGATAGAAAC TTTATCTTAC TCTACACTCA ATACAACAGA ACCACCAACA 
GGATGTGCTA TCATTACTAT CACGGATAAA ATTCAAGTTC ATCTATTGCT AAAAGGTTTG 
ATCGATTCAA AGAAGGAGAT AGAGAAACTT AGCAAAAAAC AGGAGCAATT AATAGATGTT 
ATTCATAAAA TTAAGCAAAC AATGGAAATT CCTGATTATA ATGTTAAAGT ACCTTTGGAT 
GTACAAGATA ATAACACAGA GAAATTAACA AACAATGAAG GAGAATTGCA ACGAATCATT 
GATGCATTGT CGGTATTACG GGCAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107996013 ( XM_017053864.2 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_016909353.1 Apis cerana valine--tRNA ligase (LOC107996013), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAp34934
Clone ID Related Accession (Same CDS sequence)	XM_017053864.2 , XM_017053864.1
Accession Version	XM_017053864.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-16
Organism	Apis cerana(Asiatic honeybee)
Product	valine--tRNA ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016019011.1) and transcript sequence (JR047734.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 17, 2019 this sequence version replaced XM_017053864.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis cerana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1170 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGATTGATA TGAAAACTGA GGAAATTAAA AAAGAAACAG ACGTTCCGCT GAAAAGTGCA 
AAACAGTTAC AAAAAGAAGC AAAAAAACAA GCAAAGTTGG AAAAATTTAA GCAGAAACAG 
GAAAAGAAAG AAAGCGATAA AATTCCAAAA ATTAAAGAAA AAAATGAGAA AATAGAAAAG 
AAAAAAGAAT CTAAAGAATT AGCTATATAT ACAATAAATA CACTTGAAGG AGATAAGAAA 
GATGTTACAT GTCCAATGCC TGATGCATAT AGTCCAAAAT ATGTTGAAGC TGCATGGTAT 
GCATGGTGGG AAAAACAAGG TTTTTTTAAA CCTGAATATA AAAAAAAGAA TATATTAGAG 
CCAAATCCTA AAGGAAAATT TATTATGGTG ATTCCACCAC CTAATATAAC TGGGTTTCTT 
CATCTTGGAC ATGCTTTAAC TAATGCTGTA GAAGATGCTA TTACAAGATG GAATCGAATG 
AAAGGACTTA CAACATTATG GAATCCAGGT TGTGATCATG CAGGTATTGC TACTCAGGTA 
GTAGTTGAAA AAAAACTTTG GAAAGAAGAA CAAAAAACAC GCCATGATGT AGGAAGAGAA 
AATTTTATAG AAAAAGTTTG GAAATGGAAA GAAGAAAAAG GAGACAGAAT TTACCTGCAA 
TTAAGAAAAT TAGGTGGATC ATTTGATTGG GATCGAGCTT GTTTTACAAT GGATCCAAAA 
TTGTGTCGTG CTGTTTCAGA AGCATTTATA AGATTGCATG ATGAGAATAT AATATATAGA 
AGTAATAGAT TAGTCAATTG GTCCTGCACA TTAAAAAGTG CTATTTCTGA TATAGAAGTT 
GATAAACTCG AATTAACTGG TCGAACATTA CTTTCAATTC CTGGATATCA AGAAAAAATA 
GAATTTGGAA TTTTAGTATT ATTTGCTTAT GAAGTGATAG ACTTTGAAAA AAAAATAATA 
GTTGCTACTA CTCGCATTGA AACAATGCTT GGAGATACAG CTATTGCTGT TCACCCAAAA 
GATAGTAGAT ATATTGATTT CATTGGAAGA TATGTACAAC ATCCATTTTG TGATAGAAAA 
TTACCAATTA TAGCTGATGA ATTTGTAGAA ATGGAATTCG GAACAGGTGC TGTAAAAATT 
ACACCAGCTC ATGATCCTAA TGATTACGAA GTTGGAAAGA GACATAATTT ACCATTTATA 
ACTATTTTTG ATGACACTGG AAATATTATT GGAGATTATA GACAATTTAC GGGTATGAAA 
CGATTCCATG TTCGAGCAGC TATAATAAAA GAATTAATTG CAAAAAATTT ATTCATTGAA 
ATTAAAGAGA ATCCTATGGT TGTGCCGATA TGTAGTCGAT CTAAGGATGT TGTTGAACCA 
TTAATGAAAC CTCAATGGTA TGTAAAATGT AGCGAAATGG CCTCAAAAGC AAAAGATGCA 
GTAAAATCCG GCGAATTAAA AATTATTCCG TCTCGATATA AAAAAATTTG GTATCATTGG 
ATGGAAAATA TCACAGATTG GTGTATATCT CGGCAATTAT GGTGGGGTCA TCGTATTCCT 
GCTTATTATG TTAAAATAAT TAATCCAAAT ATAAAAGTCA AGCTAGATGA TGATTATTGG 
GTAAGTGCAC ATTCAGAAAA TGAAGCTAAA AAAAAGGCTG CAAAACAGTT AGGTATATCT 
GTAGATAATA TTATTGTTGA ACAAGATCCT GATGTATTAG ATACATGGTT CTCTTCGGGT 
CTGTTTCCAT TTTCGATATT CGGATGGCCA GATAAAACTG AGGAATTTGA AGCATTTTAT 
CCTGGTACAT TATTAGAAAC TGGACATGAT ATTTTATTTT TCTGGGTAGC AAGAATGGTC 
TTTTTAGGTC AAAAGTTAAT AGGAAAATTA CCATTTAAAG AGGTTTATTT ACATGCTATG 
GTACGAGATG CTCATGGCAG AAAAATGAGT AAATCATTAG GAAATGTTAT AGATCCTATG 
GATGTAATTA ATGGGATATC ATTAGAAGAT CTTCATAAAC AATTAATAAA TTCAAATTTG 
GATCCAAAAG AATTTAAATA TGCTATTGAG GGACAAAAAC GTGATTTTCC ACAAGGGATT 
CCCGAATGTG GAACTGATGC TTTAAGATTT GCACTTTGCG CTTATACGAT GCAAGGCCGC 
GATATTAATC TTGACATTCT TCGCGTGCAG GGATATCGAT TTTTCTGTAA TAAAATATGG 
AATGCGACGA AATTCGCTCT TATGTATTTA AATTCTAATT TTGATGAAGA TGTTCAATCG 
ACAAATGATA TTGTAGTAGG TGACAATGAT TGGTATCAAA ATACTTTAAA AGAAAAATGT 
GTGCAAGAAG CATTAAATAA TTATTTGGCA GATTATTCTT ACTTAGATGG ATATAAACCT 
TCACAAATTG ATACAAAAGT TTATCAAACC TTTATCGAAC TTGATAATGA TTTTACTAAC 
TATCCTCATT TACATCGTTG GTATAAACAT ATGGCATCTT ATAATAATCA AGAAAGATCT 
ATGTTTCATG CAGAGAAAGG TAAAATATTA TCTCAATGTG GTTGTAAAAT ACATGATCAC 
AATAATAAGA AAAAACAGTT ACATTGTGAG TCAAATATAG ATTCTTGGAT GCTTTCTCGC 
GTAAGTTATG CAATAAAGAC ATGTAATGAA GCAATGACGC AATATGATTT TCCAACCGCC 
ACTACTGCTT GCTATAATCT TTGGCTATAT GATTTATGCG ATATTTATTT GGAGTATTTG 
AAACCAGTAT TTCAAAGCGA TGATGTTAAA AGAAAATATG CAGCAAAGAA AACTTTATTT 
AAAACATTAG ATATAGGATT AAGATTGTTA AGTCCTTTTA TGCCATTTAT AACAGAAGAA 
CTTTATCAAC GTTTGCCTCA TAAAAAACAA TTTTATCCTA GTATTTGTGT TAGTCCATAT 
CCAGAAGTTA CAGAGTATTC TTGGAGGAAT GAAGAAATAG AAAAAGATGT TGATTTTATC 
AATAAAGTAA TAAAAAATAT TCGATCAACG CGTGCAACAT ATAATTTACC AAATAAAATC 
AAGACTGAAG CATTTCTTGT ATGTAATTCT AATAGCTTAA AAGAAAAGCT TTTAGAATAT 
CAATTGTTGA TAGAAACTTT ATCTTACTCT ACACTCAATA CAACAGAACC ACCAACAGGA 
TGTGCTATCA TTACTATCAC GGATAAAATT CAAGTTCATC TATTGCTAAA AGGTTTGATC 
GATTCAAAGA AGGAGATAGA GAAACTTAGC AAAAAACAGG AGCAATTAAT AGATGTTATT 
CATAAAATTA AGCAAACAAT GGAAATTCCT GATTATAATG TTAAAGTACC TTTGGATGTA 
CAAGATAATA ACACAGAGAA ATTAACAAAC AATGAAGGAG AATTGCAACG AATCATTGAT 
GCATTGTCGG TATTACGGGC AATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016909353.1
CDS	132..3578
Translation	MIDMKTEEIKKETDVPLKSAKQLQKEAKKQAKLEKFKQKQEKKESDKIPKIKEKNEKIEKKKESKELAIYTINTLEGDKKDVTCPMPDAYSPKYVEAAWYAWWEKQGFFKPEYKKKNILEPNPKGKFIMVIPPPNITGFLHLGHALTNAVEDAITRWNRMKGLTTLWNPGCDHAGIATQVVVEKKLWKEEQKTRHDVGRENFIEKVWKWKEEKGDRIYLQLRKLGGSFDWDRACFTMDPKLCRAVSEAFIRLHDENIIYRSNRLVNWSCTLKSAISDIEVDKLELTGRTLLSIPGYQEKIEFGILVLFAYEVIDFEKKIIVATTRIETMLGDTAIAVHPKDSRYIDFIGRYVQHPFCDRKLPIIADEFVEMEFGTGAVKITPAHDPNDYEVGKRHNLPFITIFDDTGNIIGDYRQFTGMKRFHVRAAIIKELIAKNLFIEIKENPMVVPICSRSKDVVEPLMKPQWYVKCSEMASKAKDAVKSGELKIIPSRYKKIWYHWMENITDWCISRQLWWGHRIPAYYVKIINPNIKVKLDDDYWVSAHSENEAKKKAAKQLGISVDNIIVEQDPDVLDTWFSSGLFPFSIFGWPDKTEEFEAFYPGTLLETGHDILFFWVARMVFLGQKLIGKLPFKEVYLHAMVRDAHGRKMSKSLGNVIDPMDVINGISLEDLHKQLINSNLDPKEFKYAIEGQKRDFPQGIPECGTDALRFALCAYTMQGRDINLDILRVQGYRFFCNKIWNATKFALMYLNSNFDEDVQSTNDIVVGDNDWYQNTLKEKCVQEALNNYLADYSYLDGYKPSQIDTKVYQTFIELDNDFTNYPHLHRWYKHMASYNNQERSMFHAEKGKILSQCGCKIHDHNNKKKQLHCESNIDSWMLSRVSYAIKTCNEAMTQYDFPTATTACYNLWLYDLCDIYLEYLKPVFQSDDVKRKYAAKKTLFKTLDIGLRLLSPFMPFITEELYQRLPHKKQFYPSICVSPYPEVTEYSWRNEEIEKDVDFINKVIKNIRSTRATYNLPNKIKTEAFLVCNSNSLKEKLLEYQLLIETLSYSTLNTTEPPTGCAIITITDKIQVHLLLKGLIDSKKEIEKLSKKQEQLIDVIHKIKQTMEIPDYNVKVPLDVQDNNTEKLTNNEGELQRIIDALSVLRAI

Target ORF information:

RefSeq Version	XM_017053864.2
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana valine--tRNA ligase (LOC107996013), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017053864.2

ORF Insert Sequence:

ATGATTGATA TGAAAACTGA GGAAATTAAA AAAGAAACAG ACGTTCCGCT GAAAAGTGCA 
AAACAGTTAC AAAAAGAAGC AAAAAAACAA GCAAAGTTGG AAAAATTTAA GCAGAAACAG 
GAAAAGAAAG AAAGCGATAA AATTCCAAAA ATTAAAGAAA AAAATGAGAA AATAGAAAAG 
AAAAAAGAAT CTAAAGAATT AGCTATATAT ACAATAAATA CACTTGAAGG AGATAAGAAA 
GATGTTACAT GTCCAATGCC TGATGCATAT AGTCCAAAAT ATGTTGAAGC TGCATGGTAT 
GCATGGTGGG AAAAACAAGG TTTTTTTAAA CCTGAATATA AAAAAAAGAA TATATTAGAG 
CCAAATCCTA AAGGAAAATT TATTATGGTG ATTCCACCAC CTAATATAAC TGGGTTTCTT 
CATCTTGGAC ATGCTTTAAC TAATGCTGTA GAAGATGCTA TTACAAGATG GAATCGAATG 
AAAGGACTTA CAACATTATG GAATCCAGGT TGTGATCATG CAGGTATTGC TACTCAGGTA 
GTAGTTGAAA AAAAACTTTG GAAAGAAGAA CAAAAAACAC GCCATGATGT AGGAAGAGAA 
AATTTTATAG AAAAAGTTTG GAAATGGAAA GAAGAAAAAG GAGACAGAAT TTACCTGCAA 
TTAAGAAAAT TAGGTGGATC ATTTGATTGG GATCGAGCTT GTTTTACAAT GGATCCAAAA 
TTGTGTCGTG CTGTTTCAGA AGCATTTATA AGATTGCATG ATGAGAATAT AATATATAGA 
AGTAATAGAT TAGTCAATTG GTCCTGCACA TTAAAAAGTG CTATTTCTGA TATAGAAGTT 
GATAAACTCG AATTAACTGG TCGAACATTA CTTTCAATTC CTGGATATCA AGAAAAAATA 
GAATTTGGAA TTTTAGTATT ATTTGCTTAT GAAGTGATAG ACTTTGAAAA AAAAATAATA 
GTTGCTACTA CTCGCATTGA AACAATGCTT GGAGATACAG CTATTGCTGT TCACCCAAAA 
GATAGTAGAT ATATTGATTT CATTGGAAGA TATGTACAAC ATCCATTTTG TGATAGAAAA 
TTACCAATTA TAGCTGATGA ATTTGTAGAA ATGGAATTCG GAACAGGTGC TGTAAAAATT 
ACACCAGCTC ATGATCCTAA TGATTACGAA GTTGGAAAGA GACATAATTT ACCATTTATA 
ACTATTTTTG ATGACACTGG AAATATTATT GGAGATTATA GACAATTTAC GGGTATGAAA 
CGATTCCATG TTCGAGCAGC TATAATAAAA GAATTAATTG CAAAAAATTT ATTCATTGAA 
ATTAAAGAGA ATCCTATGGT TGTGCCGATA TGTAGTCGAT CTAAGGATGT TGTTGAACCA 
TTAATGAAAC CTCAATGGTA TGTAAAATGT AGCGAAATGG CCTCAAAAGC AAAAGATGCA 
GTAAAATCCG GCGAATTAAA AATTATTCCG TCTCGATATA AAAAAATTTG GTATCATTGG 
ATGGAAAATA TCACAGATTG GTGTATATCT CGGCAATTAT GGTGGGGTCA TCGTATTCCT 
GCTTATTATG TTAAAATAAT TAATCCAAAT ATAAAAGTCA AGCTAGATGA TGATTATTGG 
GTAAGTGCAC ATTCAGAAAA TGAAGCTAAA AAAAAGGCTG CAAAACAGTT AGGTATATCT 
GTAGATAATA TTATTGTTGA ACAAGATCCT GATGTATTAG ATACATGGTT CTCTTCGGGT 
CTGTTTCCAT TTTCGATATT CGGATGGCCA GATAAAACTG AGGAATTTGA AGCATTTTAT 
CCTGGTACAT TATTAGAAAC TGGACATGAT ATTTTATTTT TCTGGGTAGC AAGAATGGTC 
TTTTTAGGTC AAAAGTTAAT AGGAAAATTA CCATTTAAAG AGGTTTATTT ACATGCTATG 
GTACGAGATG CTCATGGCAG AAAAATGAGT AAATCATTAG GAAATGTTAT AGATCCTATG 
GATGTAATTA ATGGGATATC ATTAGAAGAT CTTCATAAAC AATTAATAAA TTCAAATTTG 
GATCCAAAAG AATTTAAATA TGCTATTGAG GGACAAAAAC GTGATTTTCC ACAAGGGATT 
CCCGAATGTG GAACTGATGC TTTAAGATTT GCACTTTGCG CTTATACGAT GCAAGGCCGC 
GATATTAATC TTGACATTCT TCGCGTGCAG GGATATCGAT TTTTCTGTAA TAAAATATGG 
AATGCGACGA AATTCGCTCT TATGTATTTA AATTCTAATT TTGATGAAGA TGTTCAATCG 
ACAAATGATA TTGTAGTAGG TGACAATGAT TGGTATCAAA ATACTTTAAA AGAAAAATGT 
GTGCAAGAAG CATTAAATAA TTATTTGGCA GATTATTCTT ACTTAGATGG ATATAAACCT 
TCACAAATTG ATACAAAAGT TTATCAAACC TTTATCGAAC TTGATAATGA TTTTACTAAC 
TATCCTCATT TACATCGTTG GTATAAACAT ATGGCATCTT ATAATAATCA AGAAAGATCT 
ATGTTTCATG CAGAGAAAGG TAAAATATTA TCTCAATGTG GTTGTAAAAT ACATGATCAC 
AATAATAAGA AAAAACAGTT ACATTGTGAG TCAAATATAG ATTCTTGGAT GCTTTCTCGC 
GTAAGTTATG CAATAAAGAC ATGTAATGAA GCAATGACGC AATATGATTT TCCAACCGCC 
ACTACTGCTT GCTATAATCT TTGGCTATAT GATTTATGCG ATATTTATTT GGAGTATTTG 
AAACCAGTAT TTCAAAGCGA TGATGTTAAA AGAAAATATG CAGCAAAGAA AACTTTATTT 
AAAACATTAG ATATAGGATT AAGATTGTTA AGTCCTTTTA TGCCATTTAT AACAGAAGAA 
CTTTATCAAC GTTTGCCTCA TAAAAAACAA TTTTATCCTA GTATTTGTGT TAGTCCATAT 
CCAGAAGTTA CAGAGTATTC TTGGAGGAAT GAAGAAATAG AAAAAGATGT TGATTTTATC 
AATAAAGTAA TAAAAAATAT TCGATCAACG CGTGCAACAT ATAATTTACC AAATAAAATC 
AAGACTGAAG CATTTCTTGT ATGTAATTCT AATAGCTTAA AAGAAAAGCT TTTAGAATAT 
CAATTGTTGA TAGAAACTTT ATCTTACTCT ACACTCAATA CAACAGAACC ACCAACAGGA 
TGTGCTATCA TTACTATCAC GGATAAAATT CAAGTTCATC TATTGCTAAA AGGTTTGATC 
GATTCAAAGA AGGAGATAGA GAAACTTAGC AAAAAACAGG AGCAATTAAT AGATGTTATT 
CATAAAATTA AGCAAACAAT GGAAATTCCT GATTATAATG TTAAAGTACC TTTGGATGTA 
CAAGATAATA ACACAGAGAA ATTAACAAAC AATGAAGGAG AATTGCAACG AATCATTGAT 
GCATTGTCGG TATTACGGGC AATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107996013 ( XM_017053864.1 ) cDNA ORF clone, Apis cerana(Asiatic honeybee) -> XP_016909353.1 Apis cerana valine--tRNA ligase (LOC107996013), mRNA.

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CloneID	OAp34934
Clone ID Related Accession (Same CDS sequence)	XM_017053864.2 , XM_017053864.1
Accession Version	XM_017053864.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-08
Organism	Apis cerana(Asiatic honeybee)
Product	valine--tRNA ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016019011.1) and transcript sequence (JR047734.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis cerana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1170 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGATTGATA TGAAAACTGA GGAAATTAAA AAAGAAACAG ACGTTCCGCT GAAAAGTGCA 
AAACAGTTAC AAAAAGAAGC AAAAAAACAA GCAAAGTTGG AAAAATTTAA GCAGAAACAG 
GAAAAGAAAG AAAGCGATAA AATTCCAAAA ATTAAAGAAA AAAATGAGAA AATAGAAAAG 
AAAAAAGAAT CTAAAGAATT AGCTATATAT ACAATAAATA CACTTGAAGG AGATAAGAAA 
GATGTTACAT GTCCAATGCC TGATGCATAT AGTCCAAAAT ATGTTGAAGC TGCATGGTAT 
GCATGGTGGG AAAAACAAGG TTTTTTTAAA CCTGAATATA AAAAAAAGAA TATATTAGAG 
CCAAATCCTA AAGGAAAATT TATTATGGTG ATTCCACCAC CTAATATAAC TGGGTTTCTT 
CATCTTGGAC ATGCTTTAAC TAATGCTGTA GAAGATGCTA TTACAAGATG GAATCGAATG 
AAAGGACTTA CAACATTATG GAATCCAGGT TGTGATCATG CAGGTATTGC TACTCAGGTA 
GTAGTTGAAA AAAAACTTTG GAAAGAAGAA CAAAAAACAC GCCATGATGT AGGAAGAGAA 
AATTTTATAG AAAAAGTTTG GAAATGGAAA GAAGAAAAAG GAGACAGAAT TTACCTGCAA 
TTAAGAAAAT TAGGTGGATC ATTTGATTGG GATCGAGCTT GTTTTACAAT GGATCCAAAA 
TTGTGTCGTG CTGTTTCAGA AGCATTTATA AGATTGCATG ATGAGAATAT AATATATAGA 
AGTAATAGAT TAGTCAATTG GTCCTGCACA TTAAAAAGTG CTATTTCTGA TATAGAAGTT 
GATAAACTCG AATTAACTGG TCGAACATTA CTTTCAATTC CTGGATATCA AGAAAAAATA 
GAATTTGGAA TTTTAGTATT ATTTGCTTAT GAAGTGATAG ACTTTGAAAA AAAAATAATA 
GTTGCTACTA CTCGCATTGA AACAATGCTT GGAGATACAG CTATTGCTGT TCACCCAAAA 
GATAGTAGAT ATATTGATTT CATTGGAAGA TATGTACAAC ATCCATTTTG TGATAGAAAA 
TTACCAATTA TAGCTGATGA ATTTGTAGAA ATGGAATTCG GAACAGGTGC TGTAAAAATT 
ACACCAGCTC ATGATCCTAA TGATTACGAA GTTGGAAAGA GACATAATTT ACCATTTATA 
ACTATTTTTG ATGACACTGG AAATATTATT GGAGATTATA GACAATTTAC GGGTATGAAA 
CGATTCCATG TTCGAGCAGC TATAATAAAA GAATTAATTG CAAAAAATTT ATTCATTGAA 
ATTAAAGAGA ATCCTATGGT TGTGCCGATA TGTAGTCGAT CTAAGGATGT TGTTGAACCA 
TTAATGAAAC CTCAATGGTA TGTAAAATGT AGCGAAATGG CCTCAAAAGC AAAAGATGCA 
GTAAAATCCG GCGAATTAAA AATTATTCCG TCTCGATATA AAAAAATTTG GTATCATTGG 
ATGGAAAATA TCACAGATTG GTGTATATCT CGGCAATTAT GGTGGGGTCA TCGTATTCCT 
GCTTATTATG TTAAAATAAT TAATCCAAAT ATAAAAGTCA AGCTAGATGA TGATTATTGG 
GTAAGTGCAC ATTCAGAAAA TGAAGCTAAA AAAAAGGCTG CAAAACAGTT AGGTATATCT 
GTAGATAATA TTATTGTTGA ACAAGATCCT GATGTATTAG ATACATGGTT CTCTTCGGGT 
CTGTTTCCAT TTTCGATATT CGGATGGCCA GATAAAACTG AGGAATTTGA AGCATTTTAT 
CCTGGTACAT TATTAGAAAC TGGACATGAT ATTTTATTTT TCTGGGTAGC AAGAATGGTC 
TTTTTAGGTC AAAAGTTAAT AGGAAAATTA CCATTTAAAG AGGTTTATTT ACATGCTATG 
GTACGAGATG CTCATGGCAG AAAAATGAGT AAATCATTAG GAAATGTTAT AGATCCTATG 
GATGTAATTA ATGGGATATC ATTAGAAGAT CTTCATAAAC AATTAATAAA TTCAAATTTG 
GATCCAAAAG AATTTAAATA TGCTATTGAG GGACAAAAAC GTGATTTTCC ACAAGGGATT 
CCCGAATGTG GAACTGATGC TTTAAGATTT GCACTTTGCG CTTATACGAT GCAAGGCCGC 
GATATTAATC TTGACATTCT TCGCGTGCAG GGATATCGAT TTTTCTGTAA TAAAATATGG 
AATGCGACGA AATTCGCTCT TATGTATTTA AATTCTAATT TTGATGAAGA TGTTCAATCG 
ACAAATGATA TTGTAGTAGG TGACAATGAT TGGTATCAAA ATACTTTAAA AGAAAAATGT 
GTGCAAGAAG CATTAAATAA TTATTTGGCA GATTATTCTT ACTTAGATGG ATATAAACCT 
TCACAAATTG ATACAAAAGT TTATCAAACC TTTATCGAAC TTGATAATGA TTTTACTAAC 
TATCCTCATT TACATCGTTG GTATAAACAT ATGGCATCTT ATAATAATCA AGAAAGATCT 
ATGTTTCATG CAGAGAAAGG TAAAATATTA TCTCAATGTG GTTGTAAAAT ACATGATCAC 
AATAATAAGA AAAAACAGTT ACATTGTGAG TCAAATATAG ATTCTTGGAT GCTTTCTCGC 
GTAAGTTATG CAATAAAGAC ATGTAATGAA GCAATGACGC AATATGATTT TCCAACCGCC 
ACTACTGCTT GCTATAATCT TTGGCTATAT GATTTATGCG ATATTTATTT GGAGTATTTG 
AAACCAGTAT TTCAAAGCGA TGATGTTAAA AGAAAATATG CAGCAAAGAA AACTTTATTT 
AAAACATTAG ATATAGGATT AAGATTGTTA AGTCCTTTTA TGCCATTTAT AACAGAAGAA 
CTTTATCAAC GTTTGCCTCA TAAAAAACAA TTTTATCCTA GTATTTGTGT TAGTCCATAT 
CCAGAAGTTA CAGAGTATTC TTGGAGGAAT GAAGAAATAG AAAAAGATGT TGATTTTATC 
AATAAAGTAA TAAAAAATAT TCGATCAACG CGTGCAACAT ATAATTTACC AAATAAAATC 
AAGACTGAAG CATTTCTTGT ATGTAATTCT AATAGCTTAA AAGAAAAGCT TTTAGAATAT 
CAATTGTTGA TAGAAACTTT ATCTTACTCT ACACTCAATA CAACAGAACC ACCAACAGGA 
TGTGCTATCA TTACTATCAC GGATAAAATT CAAGTTCATC TATTGCTAAA AGGTTTGATC 
GATTCAAAGA AGGAGATAGA GAAACTTAGC AAAAAACAGG AGCAATTAAT AGATGTTATT 
CATAAAATTA AGCAAACAAT GGAAATTCCT GATTATAATG TTAAAGTACC TTTGGATGTA 
CAAGATAATA ACACAGAGAA ATTAACAAAC AATGAAGGAG AATTGCAACG AATCATTGAT 
GCATTGTCGG TATTACGGGC AATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016909353.1
CDS	133..3579
Translation	MIDMKTEEIKKETDVPLKSAKQLQKEAKKQAKLEKFKQKQEKKESDKIPKIKEKNEKIEKKKESKELAIYTINTLEGDKKDVTCPMPDAYSPKYVEAAWYAWWEKQGFFKPEYKKKNILEPNPKGKFIMVIPPPNITGFLHLGHALTNAVEDAITRWNRMKGLTTLWNPGCDHAGIATQVVVEKKLWKEEQKTRHDVGRENFIEKVWKWKEEKGDRIYLQLRKLGGSFDWDRACFTMDPKLCRAVSEAFIRLHDENIIYRSNRLVNWSCTLKSAISDIEVDKLELTGRTLLSIPGYQEKIEFGILVLFAYEVIDFEKKIIVATTRIETMLGDTAIAVHPKDSRYIDFIGRYVQHPFCDRKLPIIADEFVEMEFGTGAVKITPAHDPNDYEVGKRHNLPFITIFDDTGNIIGDYRQFTGMKRFHVRAAIIKELIAKNLFIEIKENPMVVPICSRSKDVVEPLMKPQWYVKCSEMASKAKDAVKSGELKIIPSRYKKIWYHWMENITDWCISRQLWWGHRIPAYYVKIINPNIKVKLDDDYWVSAHSENEAKKKAAKQLGISVDNIIVEQDPDVLDTWFSSGLFPFSIFGWPDKTEEFEAFYPGTLLETGHDILFFWVARMVFLGQKLIGKLPFKEVYLHAMVRDAHGRKMSKSLGNVIDPMDVINGISLEDLHKQLINSNLDPKEFKYAIEGQKRDFPQGIPECGTDALRFALCAYTMQGRDINLDILRVQGYRFFCNKIWNATKFALMYLNSNFDEDVQSTNDIVVGDNDWYQNTLKEKCVQEALNNYLADYSYLDGYKPSQIDTKVYQTFIELDNDFTNYPHLHRWYKHMASYNNQERSMFHAEKGKILSQCGCKIHDHNNKKKQLHCESNIDSWMLSRVSYAIKTCNEAMTQYDFPTATTACYNLWLYDLCDIYLEYLKPVFQSDDVKRKYAAKKTLFKTLDIGLRLLSPFMPFITEELYQRLPHKKQFYPSICVSPYPEVTEYSWRNEEIEKDVDFINKVIKNIRSTRATYNLPNKIKTEAFLVCNSNSLKEKLLEYQLLIETLSYSTLNTTEPPTGCAIITITDKIQVHLLLKGLIDSKKEIEKLSKKQEQLIDVIHKIKQTMEIPDYNVKVPLDVQDNNTEKLTNNEGELQRIIDALSVLRAI

Target ORF information:

RefSeq Version	XM_017053864.1
Organism	Apis cerana(Asiatic honeybee)
Definition	Apis cerana valine--tRNA ligase (LOC107996013), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017053864.1

ORF Insert Sequence:

ATGATTGATA TGAAAACTGA GGAAATTAAA AAAGAAACAG ACGTTCCGCT GAAAAGTGCA 
AAACAGTTAC AAAAAGAAGC AAAAAAACAA GCAAAGTTGG AAAAATTTAA GCAGAAACAG 
GAAAAGAAAG AAAGCGATAA AATTCCAAAA ATTAAAGAAA AAAATGAGAA AATAGAAAAG 
AAAAAAGAAT CTAAAGAATT AGCTATATAT ACAATAAATA CACTTGAAGG AGATAAGAAA 
GATGTTACAT GTCCAATGCC TGATGCATAT AGTCCAAAAT ATGTTGAAGC TGCATGGTAT 
GCATGGTGGG AAAAACAAGG TTTTTTTAAA CCTGAATATA AAAAAAAGAA TATATTAGAG 
CCAAATCCTA AAGGAAAATT TATTATGGTG ATTCCACCAC CTAATATAAC TGGGTTTCTT 
CATCTTGGAC ATGCTTTAAC TAATGCTGTA GAAGATGCTA TTACAAGATG GAATCGAATG 
AAAGGACTTA CAACATTATG GAATCCAGGT TGTGATCATG CAGGTATTGC TACTCAGGTA 
GTAGTTGAAA AAAAACTTTG GAAAGAAGAA CAAAAAACAC GCCATGATGT AGGAAGAGAA 
AATTTTATAG AAAAAGTTTG GAAATGGAAA GAAGAAAAAG GAGACAGAAT TTACCTGCAA 
TTAAGAAAAT TAGGTGGATC ATTTGATTGG GATCGAGCTT GTTTTACAAT GGATCCAAAA 
TTGTGTCGTG CTGTTTCAGA AGCATTTATA AGATTGCATG ATGAGAATAT AATATATAGA 
AGTAATAGAT TAGTCAATTG GTCCTGCACA TTAAAAAGTG CTATTTCTGA TATAGAAGTT 
GATAAACTCG AATTAACTGG TCGAACATTA CTTTCAATTC CTGGATATCA AGAAAAAATA 
GAATTTGGAA TTTTAGTATT ATTTGCTTAT GAAGTGATAG ACTTTGAAAA AAAAATAATA 
GTTGCTACTA CTCGCATTGA AACAATGCTT GGAGATACAG CTATTGCTGT TCACCCAAAA 
GATAGTAGAT ATATTGATTT CATTGGAAGA TATGTACAAC ATCCATTTTG TGATAGAAAA 
TTACCAATTA TAGCTGATGA ATTTGTAGAA ATGGAATTCG GAACAGGTGC TGTAAAAATT 
ACACCAGCTC ATGATCCTAA TGATTACGAA GTTGGAAAGA GACATAATTT ACCATTTATA 
ACTATTTTTG ATGACACTGG AAATATTATT GGAGATTATA GACAATTTAC GGGTATGAAA 
CGATTCCATG TTCGAGCAGC TATAATAAAA GAATTAATTG CAAAAAATTT ATTCATTGAA 
ATTAAAGAGA ATCCTATGGT TGTGCCGATA TGTAGTCGAT CTAAGGATGT TGTTGAACCA 
TTAATGAAAC CTCAATGGTA TGTAAAATGT AGCGAAATGG CCTCAAAAGC AAAAGATGCA 
GTAAAATCCG GCGAATTAAA AATTATTCCG TCTCGATATA AAAAAATTTG GTATCATTGG 
ATGGAAAATA TCACAGATTG GTGTATATCT CGGCAATTAT GGTGGGGTCA TCGTATTCCT 
GCTTATTATG TTAAAATAAT TAATCCAAAT ATAAAAGTCA AGCTAGATGA TGATTATTGG 
GTAAGTGCAC ATTCAGAAAA TGAAGCTAAA AAAAAGGCTG CAAAACAGTT AGGTATATCT 
GTAGATAATA TTATTGTTGA ACAAGATCCT GATGTATTAG ATACATGGTT CTCTTCGGGT 
CTGTTTCCAT TTTCGATATT CGGATGGCCA GATAAAACTG AGGAATTTGA AGCATTTTAT 
CCTGGTACAT TATTAGAAAC TGGACATGAT ATTTTATTTT TCTGGGTAGC AAGAATGGTC 
TTTTTAGGTC AAAAGTTAAT AGGAAAATTA CCATTTAAAG AGGTTTATTT ACATGCTATG 
GTACGAGATG CTCATGGCAG AAAAATGAGT AAATCATTAG GAAATGTTAT AGATCCTATG 
GATGTAATTA ATGGGATATC ATTAGAAGAT CTTCATAAAC AATTAATAAA TTCAAATTTG 
GATCCAAAAG AATTTAAATA TGCTATTGAG GGACAAAAAC GTGATTTTCC ACAAGGGATT 
CCCGAATGTG GAACTGATGC TTTAAGATTT GCACTTTGCG CTTATACGAT GCAAGGCCGC 
GATATTAATC TTGACATTCT TCGCGTGCAG GGATATCGAT TTTTCTGTAA TAAAATATGG 
AATGCGACGA AATTCGCTCT TATGTATTTA AATTCTAATT TTGATGAAGA TGTTCAATCG 
ACAAATGATA TTGTAGTAGG TGACAATGAT TGGTATCAAA ATACTTTAAA AGAAAAATGT 
GTGCAAGAAG CATTAAATAA TTATTTGGCA GATTATTCTT ACTTAGATGG ATATAAACCT 
TCACAAATTG ATACAAAAGT TTATCAAACC TTTATCGAAC TTGATAATGA TTTTACTAAC 
TATCCTCATT TACATCGTTG GTATAAACAT ATGGCATCTT ATAATAATCA AGAAAGATCT 
ATGTTTCATG CAGAGAAAGG TAAAATATTA TCTCAATGTG GTTGTAAAAT ACATGATCAC 
AATAATAAGA AAAAACAGTT ACATTGTGAG TCAAATATAG ATTCTTGGAT GCTTTCTCGC 
GTAAGTTATG CAATAAAGAC ATGTAATGAA GCAATGACGC AATATGATTT TCCAACCGCC 
ACTACTGCTT GCTATAATCT TTGGCTATAT GATTTATGCG ATATTTATTT GGAGTATTTG 
AAACCAGTAT TTCAAAGCGA TGATGTTAAA AGAAAATATG CAGCAAAGAA AACTTTATTT 
AAAACATTAG ATATAGGATT AAGATTGTTA AGTCCTTTTA TGCCATTTAT AACAGAAGAA 
CTTTATCAAC GTTTGCCTCA TAAAAAACAA TTTTATCCTA GTATTTGTGT TAGTCCATAT 
CCAGAAGTTA CAGAGTATTC TTGGAGGAAT GAAGAAATAG AAAAAGATGT TGATTTTATC 
AATAAAGTAA TAAAAAATAT TCGATCAACG CGTGCAACAT ATAATTTACC AAATAAAATC 
AAGACTGAAG CATTTCTTGT ATGTAATTCT AATAGCTTAA AAGAAAAGCT TTTAGAATAT 
CAATTGTTGA TAGAAACTTT ATCTTACTCT ACACTCAATA CAACAGAACC ACCAACAGGA 
TGTGCTATCA TTACTATCAC GGATAAAATT CAAGTTCATC TATTGCTAAA AGGTTTGATC 
GATTCAAAGA AGGAGATAGA GAAACTTAGC AAAAAACAGG AGCAATTAAT AGATGTTATT 
CATAAAATTA AGCAAACAAT GGAAATTCCT GATTATAATG TTAAAGTACC TTTGGATGTA 
CAAGATAATA ACACAGAGAA ATTAACAAAC AATGAAGGAG AATTGCAACG AATCATTGAT 
GCATTGTCGG TATTACGGGC AATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC107996013 cDNA ORF clone, Apis cerana(Asiatic honeybee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム