AgaP_AGAP000178 cDNA ORF clone, Anopheles gambiae str. PEST

The following AgaP_AGAP000178 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AgaP_AGAP000178 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OAh02136 XM_310958.5
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Anopheles gambiae str. PEST AGAP000178-RA (AgaP_AGAP000178), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OAh02136
    Clone ID Related Accession (Same CDS sequence) XM_310958.5
    Accession Version XM_310958.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3963bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-25
    Organism Anopheles gambiae str. PEST
    Product AGAP000178-PA
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_004818). On Sep 28, 2011 this sequence version replaced XM_310958.4.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAAAACA TAGCACGTAC TCACGAGGAC ATCGAGTCTC ACATCCGGGA CTTGCAGGCC 
    AAGAAGAAGG AGCTGAACGC CGAAGCGGCA AAGGATAAGG GGGTAGGTTT GGCCGACAGA
    GGGTACTTCG ACATTGATCT TTACGATGGC GGTGATACCA ACAGCAAATA CGAGGGATAC
    GTCACATCGA TCGCGCCAAA CGACGACATC GACGATGAGG AGGACGAGGG CTTGCCCATC
    GGGCGGAACA ACCGACCCAT GGGTTACACG GCACCGGCGG CACTGCTCAA CGAGATGGGC
    CAGGGCGAGG ACTACGATCC GTTTGCCGAG CGACGTAAAC CGACGGTGGC GGAGAAGGAG
    GATGAATACA GACAAAAGCG AAGACGGCTG GTCATTTCAC CGGAGCGTGT CGATCCGTTT
    GCAGATGGAG GCAAAACGCC GGATGTGGGC TCGCGATCGT ACACGGAGAT TATGCGCGAA
    CAGATGCTGA AGGGCGAGGA AGCAGAGCTT CGTAAGAAAA TCCAGGAAAA GGCGAAGGAT
    GGCTCGCTGA AAATCAATTC GACCGCGCAG GCCGCGAAAC CGGCACCGGT CGAGGCGAAG
    AAGCGTGGCC GCTGGGATCA AGCAGTCGAC GAGCAGTTTG TAGCGCCGAA GAAGCTCGCC
    GTGCCAGCGA CCCCATCGTG GGATGCCGAG AAAACACCAG CCGACCACCG GTGGGACGAA
    ACGCCCGGAC ACAAGGGCAG CGAGACGCCC GGCGCCACAC CGAACGTGCG CATCTGGGAC
    GCAACACCGG CCCACGTGTC CGGTGCGGCG ACCACGCCCG GTCGGGAAAC GCCCGCCGAA
    AAGTCGACCA GGCGCAATCG CTGGGACGAG ACGCCGAAAA CCGAGCGCGA AACGCCGGGC
    CACTCGTGGG CCGAAACTCC CCGGGCCGAT CGCGTGTCGG GCGATGGCGT ACTGCTCGAG
    GGCACGACGC CCGCCTCCAA GCGACGGTCG CGCTGGGATG AAACCCCCTC GAATGCCACC
    CCGTCGGCAA TGACGCCGTC GATTGCGATG ACGCCGACAC CGCACGGCAC GACGACGCCG
    GGTCACGCGA CCCCGCTGCT CACGCCCGGC GGCACGACGC CCATCGGGCA CAAGGCGATG
    GCGATGGCAA CGCCGACGCC GGGCCATCTC GCGTCCATGA CGCCCGAGCA GCTGCAAGCG
    TACCGGTGGG AGAAGGAGAT CGACGAGCGC AACCGGCCGT TCACGGACGA GGAGCTGGAC
    GTGATGTTCC CGCCCGGGTA CAAGATTCTG CCCCCGCCGG CCGGCTACAT TCCGATCCGG
    ACGCCGGCCC GCAAGCTGAC CGCTACGCCG ACGCCGATCG CCGGCACACC GGCCGCCTTC
    TTCATCCAGA CCGAGGACAA ATCGGCCAAG TTCGTGGACA ACCAGCCCAA GGGCAACCTG
    CCGTTCATGA AGCCGGAGGA CGCGCAGTAC TTCGACAAGC TGCTGGTGGA GGTGGACGAG
    GACGCCCTCA GCCCGGAGGA GCAGAAGGAG CGCAAGATCA TGAAGCTGCT GCTGAAGATC
    AAGAACGGCA CGCCGCCGAT GCGCAAGGCG GCCCTGCGCC AGATCACCGA CAAGTCGCGC
    GAGTTCGGCG CGGGCCCGCT GTTCAACCAG ATCCTGCCGC TGCTGATGAG CCCCACGCTC
    GAGGACCAGG AGCGCCATCT GCTGGTGAAG GTGATCGACC GCATCCTGTA CAAGCTGGAC
    GATCTGGTGC GACCGTACGT GCACAAGATA CTAGTCGTGA TCGAGCCGCT GCTGATCGAC
    GAGGACTACT ATGCGCGCGT GGAGGGGCGC GAGATCATCT CGAACCTGGC GAAGGCGGCC
    GGGCTGGCCA CGATGATCTC GACGATGCGC CCGGACATTG ACAACATCGA CGAGTACGTG
    CGCAACACGA CCGCGCGCGC GTTCGCCGTC GTCGCGTCCG CGCTGGGCAT CCCGTCGCTG
    CTGCCCTTCC TGAAGGCGGT GTGCAAGAGC AAGAAGTCGT GGCAGGCGCG CCACACCGGC
    ATCAAGATCG TGCAGCAGAT CGCGATCCTG ATGGGCTGCG CCATCCTGCC CCACCTCAAG
    TCGCTGGTCG AGATCATCGA GCACGGGCTG GTGGACGAGC AGCAGAAGGT GCGCACGATC
    ACGGCCCTGG CGCTGGCGGC GCTGGCCGAG GCGGCCACGC CGTACGGCAT CGAGTCGTTC
    GACTCGGTGC TGAAGCCGCT GTGGAAGGGC ATCCGGACGC ACCGCGGCAA GGGGCTGGCC
    GCGTTCCTGA AGGCCATCGG CTACCTGATC CCGCTGATGG ACGCCGAGTA CGCGAACTAC
    TACACGCGCG AGGTGATGCT GATCCTGATC CGCGAGTTCC AGTCGCCGGA CGAGGAGATG
    AAGAAGATTG TGCTGAAGGT GGTGAAGCAG TGCTGCGCGA CGGACGGCGT GGAGGCGCAG
    TACATCAAGG AGGAGATCCT GCCGCACTTC TTCAAGCACT TCTGGAACCA CCGGATGGCG
    CTCGACCGGC GCAACTACCG GCAGCTGGTC GACACGACGG TCGAGATCGC GAACAAGGTC
    GGCGCGTCCG AGATCGTGAA CCGCGTGGTC GACGACCTCA AGGACGAGAA CGAGCAGTAC
    CGCAAGATGG TGATGGAGAC GATCGAGAAG ATCATGGCGA ACCTGGGCGC GGCCGACATC
    GACTCGCGCC TGGAGGAGCA GCTGATCGAC GGCATACTGT ACGCGTTCCA GGAGCAGACG
    ACCGAGGACG TGGTGATGCT GAACGGGTTC GGCACGATCG TGAACCAGCT GAGCAAGCGC
    GTCAAGCCGT ACCTGCCGCA GATCTGCGGC ACCATCCTGT GGCGCCTGAA CAACAAGTCG
    GCCAAGGTGC GGCAGCAGGC GGCCGACCTG ATCTCGCGCA TCGCCGTCGT GATGAAGACG
    TGCCAGGAGG AGAAGCTGAT GGGCCATCTC GGCGTGGTGC TGTACGAGTA CCTCGGCGAG
    GAGTACCCGG AGGTGCTCGG CTCGATACTG GGCGCGCTGA AGGCGATCGT GAACGTGATC
    GGCATGACGA AGATGACGCC CCCGATCAAG GATCTGCTGC CGCGGCTGAC GCCCATCCTG
    AAGAACCGGC ACGAGAAGGT GCAGGAGAAC TGCATCGATC TGGTCGGGCG CATCGCGGAC
    CGCGGCCCCG AGTACGTGTC CGCGCGCGAG TGGATGCGCA TCTGCTTCGA GCTGCTCGAG
    CTGCTGAAGG CCCACAAGAA GGCGATCCGG CGCGCGACGG TCAACACGTT CGGGTACATC
    GCGAAGGCGA TCGGGCCGCA CGACGTGCTG GCGACGCTGC TCAACAACCT GAAGGTGCAG
    GAGCGCCAGA ACCGCGTCTG CACCACGGTC GCCATCGCGA TCGTGGCGGA AACGTGCCGC
    CCGTTCACCG TGCTGCCCGC GCTCATGAAC GAGTACCGCG TGCCGGAGCT GAACGTGCAG
    AACGGCGTGC TCAAGTCGCT CTCCTTCCTG TTCGAGTACA TCGGCGAGAT GGGCAAGGAC
    TACATCTACG CCGTCTGCCC GCTGCTGGAG GACGCGCTGA TGGATCGCGA CCTCGTCCAC
    CGGCAGACGG CCTGCGCCGC GATCAAGCAC ATGGCGCTCG GCGTGTACGG GTTCGGGTGC
    GAGGACGCGC TCATCCATCT GCTGAACTAC GTCTGGCCGA ACATCTTCGA GACGTCGCCC
    CATCTGGTGC AGGCGTTCAT GGACGCGGTC GAGGGGCTGC GGGTGGCGCT CGGCCCGATC
    AAGATACTGC AGTACACGCT GCAGGGCCTG TTCCATCCCG CCCGCAAGGT GCGCGACGTC
    TACTGGAAGA TCTACAACTC GCTGTACATC GGCGCCCAGG ACGCGCTGAT CGTCGGCTAC
    CCGCGCATCA CCAACGATCC CAAGAACCAG TACATTCGGT ACGAGCTAGA GTACAACCTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_310958.5
    CDS95..4057
    Translation

    Target ORF information:

    RefSeq Version XM_310958.5
    Organism Anopheles gambiae str. PEST
    Definition Anopheles gambiae str. PEST AGAP000178-RA (AgaP_AGAP000178), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_310958.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAAAACA TAGCACGTAC TCACGAGGAC ATCGAGTCTC ACATCCGGGA CTTGCAGGCC 
    AAGAAGAAGG AGCTGAACGC CGAAGCGGCA AAGGATAAGG GGGTAGGTTT GGCCGACAGA
    GGGTACTTCG ACATTGATCT TTACGATGGC GGTGATACCA ACAGCAAATA CGAGGGATAC
    GTCACATCGA TCGCGCCAAA CGACGACATC GACGATGAGG AGGACGAGGG CTTGCCCATC
    GGGCGGAACA ACCGACCCAT GGGTTACACG GCACCGGCGG CACTGCTCAA CGAGATGGGC
    CAGGGCGAGG ACTACGATCC GTTTGCCGAG CGACGTAAAC CGACGGTGGC GGAGAAGGAG
    GATGAATACA GACAAAAGCG AAGACGGCTG GTCATTTCAC CGGAGCGTGT CGATCCGTTT
    GCAGATGGAG GCAAAACGCC GGATGTGGGC TCGCGATCGT ACACGGAGAT TATGCGCGAA
    CAGATGCTGA AGGGCGAGGA AGCAGAGCTT CGTAAGAAAA TCCAGGAAAA GGCGAAGGAT
    GGCTCGCTGA AAATCAATTC GACCGCGCAG GCCGCGAAAC CGGCACCGGT CGAGGCGAAG
    AAGCGTGGCC GCTGGGATCA AGCAGTCGAC GAGCAGTTTG TAGCGCCGAA GAAGCTCGCC
    GTGCCAGCGA CCCCATCGTG GGATGCCGAG AAAACACCAG CCGACCACCG GTGGGACGAA
    ACGCCCGGAC ACAAGGGCAG CGAGACGCCC GGCGCCACAC CGAACGTGCG CATCTGGGAC
    GCAACACCGG CCCACGTGTC CGGTGCGGCG ACCACGCCCG GTCGGGAAAC GCCCGCCGAA
    AAGTCGACCA GGCGCAATCG CTGGGACGAG ACGCCGAAAA CCGAGCGCGA AACGCCGGGC
    CACTCGTGGG CCGAAACTCC CCGGGCCGAT CGCGTGTCGG GCGATGGCGT ACTGCTCGAG
    GGCACGACGC CCGCCTCCAA GCGACGGTCG CGCTGGGATG AAACCCCCTC GAATGCCACC
    CCGTCGGCAA TGACGCCGTC GATTGCGATG ACGCCGACAC CGCACGGCAC GACGACGCCG
    GGTCACGCGA CCCCGCTGCT CACGCCCGGC GGCACGACGC CCATCGGGCA CAAGGCGATG
    GCGATGGCAA CGCCGACGCC GGGCCATCTC GCGTCCATGA CGCCCGAGCA GCTGCAAGCG
    TACCGGTGGG AGAAGGAGAT CGACGAGCGC AACCGGCCGT TCACGGACGA GGAGCTGGAC
    GTGATGTTCC CGCCCGGGTA CAAGATTCTG CCCCCGCCGG CCGGCTACAT TCCGATCCGG
    ACGCCGGCCC GCAAGCTGAC CGCTACGCCG ACGCCGATCG CCGGCACACC GGCCGCCTTC
    TTCATCCAGA CCGAGGACAA ATCGGCCAAG TTCGTGGACA ACCAGCCCAA GGGCAACCTG
    CCGTTCATGA AGCCGGAGGA CGCGCAGTAC TTCGACAAGC TGCTGGTGGA GGTGGACGAG
    GACGCCCTCA GCCCGGAGGA GCAGAAGGAG CGCAAGATCA TGAAGCTGCT GCTGAAGATC
    AAGAACGGCA CGCCGCCGAT GCGCAAGGCG GCCCTGCGCC AGATCACCGA CAAGTCGCGC
    GAGTTCGGCG CGGGCCCGCT GTTCAACCAG ATCCTGCCGC TGCTGATGAG CCCCACGCTC
    GAGGACCAGG AGCGCCATCT GCTGGTGAAG GTGATCGACC GCATCCTGTA CAAGCTGGAC
    GATCTGGTGC GACCGTACGT GCACAAGATA CTAGTCGTGA TCGAGCCGCT GCTGATCGAC
    GAGGACTACT ATGCGCGCGT GGAGGGGCGC GAGATCATCT CGAACCTGGC GAAGGCGGCC
    GGGCTGGCCA CGATGATCTC GACGATGCGC CCGGACATTG ACAACATCGA CGAGTACGTG
    CGCAACACGA CCGCGCGCGC GTTCGCCGTC GTCGCGTCCG CGCTGGGCAT CCCGTCGCTG
    CTGCCCTTCC TGAAGGCGGT GTGCAAGAGC AAGAAGTCGT GGCAGGCGCG CCACACCGGC
    ATCAAGATCG TGCAGCAGAT CGCGATCCTG ATGGGCTGCG CCATCCTGCC CCACCTCAAG
    TCGCTGGTCG AGATCATCGA GCACGGGCTG GTGGACGAGC AGCAGAAGGT GCGCACGATC
    ACGGCCCTGG CGCTGGCGGC GCTGGCCGAG GCGGCCACGC CGTACGGCAT CGAGTCGTTC
    GACTCGGTGC TGAAGCCGCT GTGGAAGGGC ATCCGGACGC ACCGCGGCAA GGGGCTGGCC
    GCGTTCCTGA AGGCCATCGG CTACCTGATC CCGCTGATGG ACGCCGAGTA CGCGAACTAC
    TACACGCGCG AGGTGATGCT GATCCTGATC CGCGAGTTCC AGTCGCCGGA CGAGGAGATG
    AAGAAGATTG TGCTGAAGGT GGTGAAGCAG TGCTGCGCGA CGGACGGCGT GGAGGCGCAG
    TACATCAAGG AGGAGATCCT GCCGCACTTC TTCAAGCACT TCTGGAACCA CCGGATGGCG
    CTCGACCGGC GCAACTACCG GCAGCTGGTC GACACGACGG TCGAGATCGC GAACAAGGTC
    GGCGCGTCCG AGATCGTGAA CCGCGTGGTC GACGACCTCA AGGACGAGAA CGAGCAGTAC
    CGCAAGATGG TGATGGAGAC GATCGAGAAG ATCATGGCGA ACCTGGGCGC GGCCGACATC
    GACTCGCGCC TGGAGGAGCA GCTGATCGAC GGCATACTGT ACGCGTTCCA GGAGCAGACG
    ACCGAGGACG TGGTGATGCT GAACGGGTTC GGCACGATCG TGAACCAGCT GAGCAAGCGC
    GTCAAGCCGT ACCTGCCGCA GATCTGCGGC ACCATCCTGT GGCGCCTGAA CAACAAGTCG
    GCCAAGGTGC GGCAGCAGGC GGCCGACCTG ATCTCGCGCA TCGCCGTCGT GATGAAGACG
    TGCCAGGAGG AGAAGCTGAT GGGCCATCTC GGCGTGGTGC TGTACGAGTA CCTCGGCGAG
    GAGTACCCGG AGGTGCTCGG CTCGATACTG GGCGCGCTGA AGGCGATCGT GAACGTGATC
    GGCATGACGA AGATGACGCC CCCGATCAAG GATCTGCTGC CGCGGCTGAC GCCCATCCTG
    AAGAACCGGC ACGAGAAGGT GCAGGAGAAC TGCATCGATC TGGTCGGGCG CATCGCGGAC
    CGCGGCCCCG AGTACGTGTC CGCGCGCGAG TGGATGCGCA TCTGCTTCGA GCTGCTCGAG
    CTGCTGAAGG CCCACAAGAA GGCGATCCGG CGCGCGACGG TCAACACGTT CGGGTACATC
    GCGAAGGCGA TCGGGCCGCA CGACGTGCTG GCGACGCTGC TCAACAACCT GAAGGTGCAG
    GAGCGCCAGA ACCGCGTCTG CACCACGGTC GCCATCGCGA TCGTGGCGGA AACGTGCCGC
    CCGTTCACCG TGCTGCCCGC GCTCATGAAC GAGTACCGCG TGCCGGAGCT GAACGTGCAG
    AACGGCGTGC TCAAGTCGCT CTCCTTCCTG TTCGAGTACA TCGGCGAGAT GGGCAAGGAC
    TACATCTACG CCGTCTGCCC GCTGCTGGAG GACGCGCTGA TGGATCGCGA CCTCGTCCAC
    CGGCAGACGG CCTGCGCCGC GATCAAGCAC ATGGCGCTCG GCGTGTACGG GTTCGGGTGC
    GAGGACGCGC TCATCCATCT GCTGAACTAC GTCTGGCCGA ACATCTTCGA GACGTCGCCC
    CATCTGGTGC AGGCGTTCAT GGACGCGGTC GAGGGGCTGC GGGTGGCGCT CGGCCCGATC
    AAGATACTGC AGTACACGCT GCAGGGCCTG TTCCATCCCG CCCGCAAGGT GCGCGACGTC
    TACTGGAAGA TCTACAACTC GCTGTACATC GGCGCCCAGG ACGCGCTGAT CGTCGGCTAC
    CCGCGCATCA CCAACGATCC CAAGAACCAG TACATTCGGT ACGAGCTAGA GTACAACCTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The Anopheles gambiae genome: an update.
    Trends in parasitology20(2)49-52(2004 Feb)
    Mongin E,Louis C,Holt RA,Birney E,Collins FH


    The genome sequence of the malaria mosquito Anopheles gambiae.
    Science (New York, N.Y.)298(5591)129-49(2002 Oct)
    Holt RA,Subramanian GM,Halpern A,Sutton GG,Charlab R,Nusskern DR,Wincker P,Clark AG,Ribeiro JM,Wides R,Salzberg SL,Loftus B,Yandell M,Majoros WH,Rusch DB,Lai Z,Kraft CL,Abril JF,Anthouard V,Arensburger P,Atkinson PW,Baden H,de Berardinis V,Baldwin D,Benes V,Biedler J,Blass C,Bolanos R,Boscus D,Barnstead M,Cai S,Center A,Chaturverdi K,Christophides GK,Chrystal MA,Clamp M,Cravchik A,Curwen V,Dana A,Delcher A,Dew I,Evans CA,Flanigan M,Grundschober-Freimoser A,Friedli L,Gu Z,Guan P,Guigo R,Hillenmeyer ME,Hladun SL,Hogan JR,Hong YS,Hoover J,Jaillon O,Ke Z,Kodira C,Kokoza E,Koutsos A,Letunic I,Levitsky A,Liang Y,Lin JJ,Lobo NF,Lopez JR,Malek JA,McIntosh TC,Meister S,Miller J,Mobarry C,Mongin E,Murphy SD,O'Brochta DA,Pfannkoch C,Qi R,Regier MA,Remington K,Shao H,Sharakhova MV,Sitter CD,Shetty J,Smith TJ,Strong R,Sun J,Thomasova D,Ton LQ,Topalis P,Tu Z,Unger MF,Walenz B,Wang A,Wang J,Wang M,Wang X,Woodford KJ,Wortman JR,Wu M,Yao A,Zdobnov EM,Zhang H,Zhao Q,Zhao S,Zhu SC,Zhimulev I,Coluzzi M,della Torre A,Roth CW,Louis C,Kalush F,Mural RJ,Myers EW,Adams MD,Smith HO,Broder S,Gardner MJ,Fraser CM,Birney E,Bork P,Brey PT,Venter JC,Weissenbach J,Kafatos FC,Collins FH,Hoffman SL