AgaP_AGAP000020 cDNA ORF clone, Anopheles gambiae str. PEST

Gene
Clones
gRNAs

Summary

Gene Symbol	AgaP_AGAP000020
Entrez Gene ID	3289598
Full Name	AGAP000020-PA
Synonyms	AgaP_ENSANGG00000023578,ENSANGG00000023578
Gene Type	protein-coding
Organism	Anopheles gambiae str. PEST

mRNA and Protein(s)

mRNA	Protein	Name
XM_566411.4	XP_566411.4	AGAP000020-PA

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The Anopheles gambiae genome: an update.
Mongin E, Louis C, Holt RA, Birney E, Collins FH
Trends in parasitology20(2)49-52(2004 Feb)

The genome sequence of the malaria mosquito Anopheles gambiae.
Holt RA, Subramanian GM, Halpern A, Sutton GG, Charlab R, Nusskern DR, Wincker P, Clark AG, Ribeiro JM, Wides R, Salzberg SL, Loftus B, Yandell M, Majoros WH, Rusch DB, Lai Z, Kraft CL, Abril JF, Anthouard V, Arensburger P, Atkinson PW, Baden H, de Berardinis V, Baldwin D, Benes V, Biedler J, Blass C, Bolanos R, Boscus D, Barnstead M, Cai S, Center A, Chaturverdi K, Christophides GK, Chrystal MA, Clamp M, Cravchik A, Curwen V, Dana A, Delcher A, Dew I, Evans CA, Flanigan M, Grundschober-Freimoser A, Friedli L, Gu Z, Guan P, Guigo R, Hillenmeyer ME, Hladun SL, Hogan JR, Hong YS, Hoover J, Jaillon O, Ke Z, Kodira C, Kokoza E, Koutsos A, Letunic I, Levitsky A, Liang Y, Lin JJ, Lobo NF, Lopez JR, Malek JA, McIntosh TC, Meister S, Miller J, Mobarry C, Mongin E, Murphy SD, O'Brochta DA, Pfannkoch C, Qi R, Regier MA, Remington K, Shao H, Sharakhova MV, Sitter CD, Shetty J, Smith TJ, Strong R, Sun J, Thomasova D, Ton LQ, Topalis P, Tu Z, Unger MF, Walenz B, Wang A, Wang J, Wang M, Wang X, Woodford KJ, Wortman JR, Wu M, Yao A, Zdobnov EM, Zhang H, Zhao Q, Zhao S, Zhu SC, Zhimulev I, Coluzzi M, della Torre A, Roth CW, Louis C, Kalush F, Mural RJ, Myers EW, Adams MD, Smith HO, Broder S, Gardner MJ, Fraser CM, Birney E, Bork P, Brey PT, Venter JC, Weissenbach J, Kafatos FC, Collins FH, Hoffman SL
Science (New York, N.Y.)298(5591)129-49(2002 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AgaP_AGAP000020 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AgaP_AGAP000020 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAh07849	XM_566411.4 Latest version!	Anopheles gambiae str. PEST AGAP000020-RA (AgaP_AGAP000020), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AgaP_AGAP000020 ( XM_566411.4 ) cDNA ORF clone, Anopheles gambiae str. PEST -> XP_566411.4 Anopheles gambiae str. PEST AGAP000020-RA (AgaP_AGAP000020), partial mRNA.

Price & Availability↑

CloneID	OAh07849
Clone ID Related Accession (Same CDS sequence)	XM_566411.4
Accession Version	XM_566411.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-25
Organism	Anopheles gambiae str. PEST
Product	AGAP000020-PA
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_004818). On Sep 28, 2011 this sequence version replaced XM_566411.3. COMPLETENESS: incomplete on both ends.

ATGGGTGGCG ATTTTGCTCC CACTGCTCCG CCCTGCAGTG CTCCAGCCGT CCCAAACCTG 
TCCACCACCA TGCTTTATCC CGATTTGAGC CAACTAAAGC TGGAAGCGAG CGACGGCGTG 
CAAACGTTAG TACGTCCTTC AACCACCAGC GCGCAACCAA TCGAACGGCC CTGCATTAGT 
GCACCGCGAA CCCGCACACT CTACCTGCAA TGCATCCAGC AGATGGAGGA TACCGCCCTA 
CAGACCGACC GGGCGCATCT GGACGCGATG GAGCAGCAGT TCGTCCGGTC CGAAAGCCCG 
CACGGCGTGG CGAAGGATCC GGGTGGGTAC GATGACGACC TGCACATAAG CATACAGACG 
TATCGGGCCG GCTACCACGG CTACGGCGAG GTCGTGCGGG AGCGCAAGGT GGCCCACCAG 
CAAATAGCCA GCCTGCAGGG TAGATGCTGG GACTTCCAAC AGGTAAAGTT CAGTGCGACG 
GGCCGGTGCG ACGACGAGCG GACGGTGAGT GTGTCGCTGA AAAGCAGAGT CGCCTCGCTG 
AACGTAGAAC GGTTTAAGGA GGCGAAGGGT ACCATGTCCT CGCTGCTGGA TCGCTGTCTG 
ACGAAGGAGA AGGAAGCACT GATACACCTG CTCCACACTC GCTCGATGGT GGAGAAGAAG 
GTCTCGGACC CGCCGAAGGC CGGCCAAAAT CCAACCGTGG CGCTACGCCA CAAGCTACGC 
ATCATTGGCA ATGCGCTGCG CATGGAAGCG TCCGGTAGCG GGTGCGACGA GGGCGAACGA 
TGCGAGTACG TGCAGGATTT GCGCAAGTGG TTTGTGCACG TCGGCACCAA CCTGTTGGGC 
GCGGGCAACG TGCACGACCG GGTCTGGCTG ATGTTTCATT TGCTGCGCTT TCCCAGCGGT 
ATCGGCACGT GGGCACACGT GCTGGTACAT CCGCTACCGG TCGGAGGTAA AGAATCGAGC 
CCCGATCTGC TGGACGGTGA GCTGCAGGCC ATTCTTACCC TGACGCACGT GCTGCTGCGT 
CCCATCCTCG AGCGCCAAAC ATTCCTAACG CCGCTGGAGG AAGCGGAGGG CCGTACCGCT 
CAAGAGAAGA TGCCGGTTAG AGAGGCGGGT GGCAGTGGGT CGAAAGCGGC CGACAACTTC 
GAATGGGTCG ATAGCGACGG GGAGGAAAGT TCGCCGGACA AGCGGGTGCG GCCGATTAAG 
GAAAGCGATC TGCTCGCACT GCTTGACCAG ATACCGTTCC AGCGGCTGTT TGGTACGATT 
ACGTCACGCG CGATTCGCCG CGTGGTGAGC GGAGACGTGC GGCAGCTGCC CACCACCGAA 
ATGCTGTGGC TAGTCACGCT CGCCAACCGG CTGGTAGCCG TACTCGGCGA GGGATTGTCG 
ACGTACGGTC GGATCGCCAG ATACAGCCAC TTTGCGCAAC GTTTGGCGCT GCTCATCAAC 
GATACGGTCA AGTTCGCGGG TGACGTGCTG CGCTACTATC GCGACACACA CGGGCCGCAT 
ACGTCCGAGG AGGTGGAGCT GGTGCAGGTA CACTTCGATA CGCTCGTGTA CGGTGCGGCC 
CGTTGCATCT ACGCAGCAGG CGTGGCCCTG GCCCGGCACC TTTCGACGCT GCCCTTCGCG 
TTGCTCAGCT CGCGCGCTTG CTGGTGGATG CTGCGTTGCC TTGTGCAGCG CGACTTTACG 
CTTGGGCCAG ACTTTGAGCA TGAAAGTAAG CCACCCGTCC CGCACAATGC CACCACTACG 
GACCAGTTCA GCAAAGCGTT GCTCGGCCAG GAGGCAGCGC ACAATCTGTA CAACGTGCTG 
AAACCATTCG TCGATCTTGC ACTCGCACGG GATCCATCCA GCGATAAGGA GTTCATCTGC 
ACAATCACGA AAACACTGTT TGAGGTGCTG GTTGCTGTCG ATGCGGCCGA ACTCGTCTGT 
GCCGAGCTCA TAATCGAGCA GATTCATACG ATCGTGGAGA AAAATGTGTT TGTGCTTTCG 
GTAATGCTAC AAACGCTAGC GGCGGACGGC ACGGCTAACC CTCAGTCCAC CGCCGATGCT 
TGGCAGGCGA AGGGGAGCAG ACTGCTGGAC GTATTCAGTA GCAAAAGGAA GTTGGTTATG 
CTGTGGGATC CAACTGAATC AGATGTAAAC GTGATGCTGG AGCTGCTGCT AAGTTATTCC 
AGAGCGCACG TGTACCACAA GCTAGCGCTG GGGCTGTTAA TTGGGTTGAA TGTGCCAAAG 
GCGCTACAAA ACCGCGTAGC GTACAGCGTC GTGGCGGCGT TTCGCAAAAA TCAATCTCCG 
GTCAAAGCAA ACTCCGACGC TACGGAGCAG AGCGAATATG AAAACTACCA GGAGCGTTGC 
CTGTTTGTGC TGCTGCAGCT GCGATTGCAC GCACTCGACC AGCCGCTGCC CATCGTGCAG 
CAGATCTTGC AAGATCCCTC CAGCGTGGAG GTGCGCAATG TGCCCGCTCT CGAGCAGCTA 
GCGGACGTGA AGGCAGCGGT TGACGAACGG TGCCCGGTTG CGTGTCTGGC CGCGATGCTT 
ACCACCACCA TCGGACACTG GGTGCCCGTC TTCTGCCAGG ACGGCGTGAA CTTGTTGCAG 
CTGCTGCTAG ATCACCACCT CGACACGGTT GTGGTGCGCT GCCTGGAGCT GATCAGTCTG 
CTGTTTACCG AGTGTTCCCA TGCGCTGCTG GGTAGCGAGC GATTCGCCGA CCTGCTGACA 
CAGCTGGTGC AGGATGACGT TGAGCTAGTT TGGACGCCGC CGAAGCCTTC CACCAGCACG 
ATCGATCCGC AACAGAGCGA ACAGATGCTC GCGCTGAAGC TGCTTGATCA GGCGAAGGTG 
GGCAAAGTGG GCACGATGCT GGTGTCGCAA GCGGTTTCGC ACTGGCGCTG TGGCTACGCA 
ACGCCGGCGG CGTTAGTCGG CATGTGGTGC GACTGGTTGA CGCAAATATC CAGCTGGACG 
AACAGTGCCA AGGTGCTGCG TTTGCTGAAC GTGCTCGCCT CGGTTTCGTT CGGCCACGCG 
GAAGCCTGGA AGGCGTTGCG CGATAAGCTG GCTCCATTTT TCAAGTCCTT CGCGCAGGTG 
AAACAAAAGC AACCGGGCAT ACACTCGCTC TGGTCTAAGA TAATCGGTAC CGAGCCGCCG 
CTGCTGTACG GCACCCTGCC AAACGATTGC GTGGCCCTGG CGCTGCTTGT GTTCGAGCTC 
GAGCACCGAC AGCTCGAACT GGACACCGAA CTCTGGCCCA AGCTGCTGCG TGCACTCAAG 
CGGAAGGAAT CGCTCAAGCT GGACGCGGCA CTGCGCGACG TGAGCGGAGG CCTGCGCACG 
CGCGAGGAAG ACTTTTGTCC TGCGGCCGAT TCGCTGGTCT ACTGCAAGCT GGCCCGCTTT 
CTGGTGCGCG TCAGCGTGGA GCATCCACTG TGTCTGGCCG TCTGGCAGCA GTTTTTCGCC 
GCCCTGCTGA CGCGCGTCAC GGACGTGGGC GACCCGGTGC ACGGTGTGTC GGATCGACTG 
TACGGCGCGG ACACCGGTCT GATGGAAAAG GTGAAACAGA CGCTGTGCCG TCTCGAGCTG 
CACTACCATA GCGCGCAAGA GACGAACAAA CCCGCGGTGG AAATGTTGCG TCTCGTCAAA 
GCGTTCCAGC TGTGGTTGCT GGACCCGGAC CTGAATCGCA TCGCGATGGA CGATCCGATC 
AATCTACCAG TGCAGTACGC CGTTCCCCAT CTGCAGGCGA CTCTGCAGGG CGTGCAGGAA 
TGTTGGCCCG AGTGCGTCAA CCAGCCGGCC CTCGTCACGC TGCACCGTCT GATGGTGGAG 
CGCTGGTACG CGCTTTACCG CGTCCATCCG GCCCAACCGC TAAAGGCCCA ACCGTCGGAA 
GGGACTGCTG CCGGCAAACC GGCGCTTACG CCAGTCGAGG CCATCCAGCG CCGGCTAGCA 
AACGCGTACA CCGATCCACT CCCAGTGCCG GTGGTCGAGG TGCGCGGCGA GCAGCAGGAT 
TTGCGCGACG CACTGGCGAA ACCGGCGGCG GTACGAGTCA GGCGCATCAC CGAGAGCCTG 
CGGATCGTGC GGCAGCACAT CGACAAAACG TACTGGACGA CGAAAGCCGA GCTGGAGCAG 
CGCCGGGACG AGCTGTTCGA GCTGTACCGG CAGCTGTACG TCAACGAGGA CAGGCAGCTG 
GTGAAGCAGG CCAAGTGTAG CGTGCTGTAC TGTGCCGGTG CCGCCACGGT CAAGGTGCAC 
ACGAAGTGTG CCACGCTGGA CAAGACCGTG CAGGAGCGCA TCGACGCACG GTTGCGCGGG 
CTGGAAGCGT TCCTGCAGAT GGCGATCAAC ATTCCACCGT ACATCGTCCA CCACACCATC 
TTGCTGCGCG AGCTGTGGAA CAGTCTGTTC GTCGACTACT GCGCCGAGAC GGACCAGACC 
GCGGTCCAGT CGCTGAATGC CATCATCCGG ACGATGCTGC GCACGCTGCT GCACGAGGTG 
TCCGATGCGA ACTTCGTGCC GCCGCTTACA TTCGCCGTTC GGCTAAGCCT CGAGACGTAC 
CGGAGCGAAC TGTCGAAGCT TATGTTCGAG GAGGTCGGGC AGCTGTTTGC GGACGCGCTC 
GAGACAGGAC ACAAACCGTC CAGCGTGATC GTTGCCCTGC TAGAGGACGG GCGCATTCCG 
TGCCGGCCGC TGCTGCAGGT GTACGGCCAG CTGGTGAAGC AGAAAACCGG CAGCCTGGAG 
CGGACCCTGT TTGTGAACAT GTTCGGCAGC AAGCTCGACC TGTCGCGGTG GCTAAAGGAG 
CATGACGTCG CGCCGGACGA GCTCGACCAG TTTGCCAAGC TGATAGTGAT CGGACTGTAC 
AAAGCGCGGC CCGCGGATAC CGTCAGCCCG GTGCGATCGG ACGAGCAAGC CGAGCAGGAT 
GAAAAGTTTG CAGACATCCT AATTTCGCAC CTGGTAACGT TGGCGAGCAG CAAATTTCCC 
GACAACTTTG GCAAACTGCT GCAGCACACG ATCAACGCGT ACTCGCAATG GCCCACGCTG 
CCGCCGGTGA TGCTGCTACG GTTGCTGAAC GTGCTGCGTT CGCGGGCCCA CCATCTGCCC 
GATCTGTGCC TGGGCATGGC GGAGGACGCA CTGCACCAGG CCCATCGCCA GTTTGCCGAA 
GCGAACTGCA CCGAGCCGGT GCTGGGGCCG GAACTGTTGC ACCATCTGCT GGTGGCCGTG 
ACCGAGCACT TTGTGCGCCA GCGCGATGGC GCTTACCCAT GGAACGGCAT GTACAGGCGG 
CACGGCGCGT ACATCGAGGT GCTGGGAATG CTGCTGGGCA TGTTTGCCCA CAGCCTGCTT 
GCCGCCGGCA TGCGTGACAA TCGCATCGGA TGCGTGCACG ACAACCTGCT GCCCACCGTG 
TATCGCATGT TTGAGCCGTG GATCGTACCG TACGGTGCGG ACGTCCATGC ACACGCCCGG 
CCCGGTGGAT ACTCGCCGTC CACACCGTAC GGCAGGACGA CCATGGCGCC CGTGATCGAC 
ACAGCGCGAC ACTGCAACAA CGATAAGGCC AAGTGGATGT TCGGCGTGCT GCTGCACTGC 
GTCGAGTACG CAATCGAGCA GGTGCGCGAA GCGTACGCCG ACACCGACCA TTGCAGCCAG 
ATACTGCTGT ACTTCCTTCA CTGGTACCTG GAATGGTTCG TCGATCCGCG CATCATGATT 
TCGGCCCTGT ACGTGTACAA CACGCTCGTG CTCGATCTGC CCTGGGAGCG GCTGCTGCCG 
TCGGAGCTGC TGCTCGAGCG TATGCACGCC ATGCTGGAGC ACCACAGCCC CGAGTGCCAC 
GAGCTGCTGG CGTGCGTGTT CGTGCGTTGC CGCTGGTTCG GCGAACCGTT CGATCCCCTG 
CCCGGCTGGA TACGGCGCAC GCACGCCCCT ACGCTCGCGA TCTGCGTACG GCTAGCGTAC 
GAGCCGGTGG TACGGTCGGA GGAGCAAACG CGCACGGCCA TGGTGCGGCT GCTGCACCAC 
TTTGCCCGCC TCGTGTGGAT GCCGATGCAC GTTGCCGAGC TGACGCCAGC ACTGGACTGG 
TTCGTAATGA CCGCGGAAGC AGGCTTTCTG CTGCGCGAGC CGAAGGTGCC GCACCGCGAC 
CTGGACGATG CGCTCATGCT CTTGCTTGAA ATCGTGACGG GAATGCGGTG CAACGAAGCG 
AACCAGCTCG TGCCGGGCGG CATGTATCTG TTGAAGCGCA AGCTGTACAT CGGCGTCGTC 
GTGCGGATGC TCATGAATGC CGGCCGGGCG GCAAGCAAGG TGGCGAACGG GAAGCCCCAG 
CTGACCGCGG CCGTACAGCG GCTGGTTGCG TCGATAGCGG ATGTACTGGC ACAATTGCCG 
GACGCGTGCG GCACCGGCTG CTCGCACTGT CCCCGGCTGC TCGAGGCGCG CGCCTTGATG 
ACTGAGCTGC TGACCAGTGT CAAAAAGTGG CAAACCGAAA ATACGCTAAG GTACAGTTCT 
TTACCCATCC TAACCTCATT TACCCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_566411.4
CDS	1..6450
Translation	MGGDFAPTAPPCSAPAVPNLSTTMLYPDLSQLKLEASDGVQTLVRPSTTSAQPIERPCISAPRTRTLYLQCIQQMEDTALQTDRAHLDAMEQQFVRSESPHGVAKDPGGYDDDLHISIQTYRAGYHGYGEVVRERKVAHQQIASLQGRCWDFQQVKFSATGRCDDERTVSVSLKSRVASLNVERFKEAKGTMSSLLDRCLTKEKEALIHLLHTRSMVEKKVSDPPKAGQNPTVALRHKLRIIGNALRMEASGSGCDEGERCEYVQDLRKWFVHVGTNLLGAGNVHDRVWLMFHLLRFPSGIGTWAHVLVHPLPVGGKESSPDLLDGELQAILTLTHVLLRPILERQTFLTPLEEAEGRTAQEKMPVREAGGSGSKAADNFEWVDSDGEESSPDKRVRPIKESDLLALLDQIPFQRLFGTITSRAIRRVVSGDVRQLPTTEMLWLVTLANRLVAVLGEGLSTYGRIARYSHFAQRLALLINDTVKFAGDVLRYYRDTHGPHTSEEVELVQVHFDTLVYGAARCIYAAGVALARHLSTLPFALLSSRACWWMLRCLVQRDFTLGPDFEHESKPPVPHNATTTDQFSKALLGQEAAHNLYNVLKPFVDLALARDPSSDKEFICTITKTLFEVLVAVDAAELVCAELIIEQIHTIVEKNVFVLSVMLQTLAADGTANPQSTADAWQAKGSRLLDVFSSKRKLVMLWDPTESDVNVMLELLLSYSRAHVYHKLALGLLIGLNVPKALQNRVAYSVVAAFRKNQSPVKANSDATEQSEYENYQERCLFVLLQLRLHALDQPLPIVQQILQDPSSVEVRNVPALEQLADVKAAVDERCPVACLAAMLTTTIGHWVPVFCQDGVNLLQLLLDHHLDTVVVRCLELISLLFTECSHALLGSERFADLLTQLVQDDVELVWTPPKPSTSTIDPQQSEQMLALKLLDQAKVGKVGTMLVSQAVSHWRCGYATPAALVGMWCDWLTQISSWTNSAKVLRLLNVLASVSFGHAEAWKALRDKLAPFFKSFAQVKQKQPGIHSLWSKIIGTEPPLLYGTLPNDCVALALLVFELEHRQLELDTELWPKLLRALKRKESLKLDAALRDVSGGLRTREEDFCPAADSLVYCKLARFLVRVSVEHPLCLAVWQQFFAALLTRVTDVGDPVHGVSDRLYGADTGLMEKVKQTLCRLELHYHSAQETNKPAVEMLRLVKAFQLWLLDPDLNRIAMDDPINLPVQYAVPHLQATLQGVQECWPECVNQPALVTLHRLMVERWYALYRVHPAQPLKAQPSEGTAAGKPALTPVEAIQRRLANAYTDPLPVPVVEVRGEQQDLRDALAKPAAVRVRRITESLRIVRQHIDKTYWTTKAELEQRRDELFELYRQLYVNEDRQLVKQAKCSVLYCAGAATVKVHTKCATLDKTVQERIDARLRGLEAFLQMAINIPPYIVHHTILLRELWNSLFVDYCAETDQTAVQSLNAIIRTMLRTLLHEVSDANFVPPLTFAVRLSLETYRSELSKLMFEEVGQLFADALETGHKPSSVIVALLEDGRIPCRPLLQVYGQLVKQKTGSLERTLFVNMFGSKLDLSRWLKEHDVAPDELDQFAKLIVIGLYKARPADTVSPVRSDEQAEQDEKFADILISHLVTLASSKFPDNFGKLLQHTINAYSQWPTLPPVMLLRLLNVLRSRAHHLPDLCLGMAEDALHQAHRQFAEANCTEPVLGPELLHHLLVAVTEHFVRQRDGAYPWNGMYRRHGAYIEVLGMLLGMFAHSLLAAGMRDNRIGCVHDNLLPTVYRMFEPWIVPYGADVHAHARPGGYSPSTPYGRTTMAPVIDTARHCNNDKAKWMFGVLLHCVEYAIEQVREAYADTDHCSQILLYFLHWYLEWFVDPRIMISALYVYNTLVLDLPWERLLPSELLLERMHAMLEHHSPECHELLACVFVRCRWFGEPFDPLPGWIRRTHAPTLAICVRLAYEPVVRSEEQTRTAMVRLLHHFARLVWMPMHVAELTPALDWFVMTAEAGFLLREPKVPHRDLDDALMLLLEIVTGMRCNEANQLVPGGMYLLKRKLYIGVVVRMLMNAGRAASKVANGKPQLTAAVQRLVASIADVLAQLPDACGTGCSHCPRLLEARALMTELLTSVKKWQTENTLRYSSLPILTSFTR

Target ORF information:

RefSeq Version	XM_566411.4
Organism	Anopheles gambiae str. PEST
Definition	Anopheles gambiae str. PEST AGAP000020-RA (AgaP_AGAP000020), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_566411.4

ORF Insert Sequence:

ATGGGTGGCG ATTTTGCTCC CACTGCTCCG CCCTGCAGTG CTCCAGCCGT CCCAAACCTG 
TCCACCACCA TGCTTTATCC CGATTTGAGC CAACTAAAGC TGGAAGCGAG CGACGGCGTG 
CAAACGTTAG TACGTCCTTC AACCACCAGC GCGCAACCAA TCGAACGGCC CTGCATTAGT 
GCACCGCGAA CCCGCACACT CTACCTGCAA TGCATCCAGC AGATGGAGGA TACCGCCCTA 
CAGACCGACC GGGCGCATCT GGACGCGATG GAGCAGCAGT TCGTCCGGTC CGAAAGCCCG 
CACGGCGTGG CGAAGGATCC GGGTGGGTAC GATGACGACC TGCACATAAG CATACAGACG 
TATCGGGCCG GCTACCACGG CTACGGCGAG GTCGTGCGGG AGCGCAAGGT GGCCCACCAG 
CAAATAGCCA GCCTGCAGGG TAGATGCTGG GACTTCCAAC AGGTAAAGTT CAGTGCGACG 
GGCCGGTGCG ACGACGAGCG GACGGTGAGT GTGTCGCTGA AAAGCAGAGT CGCCTCGCTG 
AACGTAGAAC GGTTTAAGGA GGCGAAGGGT ACCATGTCCT CGCTGCTGGA TCGCTGTCTG 
ACGAAGGAGA AGGAAGCACT GATACACCTG CTCCACACTC GCTCGATGGT GGAGAAGAAG 
GTCTCGGACC CGCCGAAGGC CGGCCAAAAT CCAACCGTGG CGCTACGCCA CAAGCTACGC 
ATCATTGGCA ATGCGCTGCG CATGGAAGCG TCCGGTAGCG GGTGCGACGA GGGCGAACGA 
TGCGAGTACG TGCAGGATTT GCGCAAGTGG TTTGTGCACG TCGGCACCAA CCTGTTGGGC 
GCGGGCAACG TGCACGACCG GGTCTGGCTG ATGTTTCATT TGCTGCGCTT TCCCAGCGGT 
ATCGGCACGT GGGCACACGT GCTGGTACAT CCGCTACCGG TCGGAGGTAA AGAATCGAGC 
CCCGATCTGC TGGACGGTGA GCTGCAGGCC ATTCTTACCC TGACGCACGT GCTGCTGCGT 
CCCATCCTCG AGCGCCAAAC ATTCCTAACG CCGCTGGAGG AAGCGGAGGG CCGTACCGCT 
CAAGAGAAGA TGCCGGTTAG AGAGGCGGGT GGCAGTGGGT CGAAAGCGGC CGACAACTTC 
GAATGGGTCG ATAGCGACGG GGAGGAAAGT TCGCCGGACA AGCGGGTGCG GCCGATTAAG 
GAAAGCGATC TGCTCGCACT GCTTGACCAG ATACCGTTCC AGCGGCTGTT TGGTACGATT 
ACGTCACGCG CGATTCGCCG CGTGGTGAGC GGAGACGTGC GGCAGCTGCC CACCACCGAA 
ATGCTGTGGC TAGTCACGCT CGCCAACCGG CTGGTAGCCG TACTCGGCGA GGGATTGTCG 
ACGTACGGTC GGATCGCCAG ATACAGCCAC TTTGCGCAAC GTTTGGCGCT GCTCATCAAC 
GATACGGTCA AGTTCGCGGG TGACGTGCTG CGCTACTATC GCGACACACA CGGGCCGCAT 
ACGTCCGAGG AGGTGGAGCT GGTGCAGGTA CACTTCGATA CGCTCGTGTA CGGTGCGGCC 
CGTTGCATCT ACGCAGCAGG CGTGGCCCTG GCCCGGCACC TTTCGACGCT GCCCTTCGCG 
TTGCTCAGCT CGCGCGCTTG CTGGTGGATG CTGCGTTGCC TTGTGCAGCG CGACTTTACG 
CTTGGGCCAG ACTTTGAGCA TGAAAGTAAG CCACCCGTCC CGCACAATGC CACCACTACG 
GACCAGTTCA GCAAAGCGTT GCTCGGCCAG GAGGCAGCGC ACAATCTGTA CAACGTGCTG 
AAACCATTCG TCGATCTTGC ACTCGCACGG GATCCATCCA GCGATAAGGA GTTCATCTGC 
ACAATCACGA AAACACTGTT TGAGGTGCTG GTTGCTGTCG ATGCGGCCGA ACTCGTCTGT 
GCCGAGCTCA TAATCGAGCA GATTCATACG ATCGTGGAGA AAAATGTGTT TGTGCTTTCG 
GTAATGCTAC AAACGCTAGC GGCGGACGGC ACGGCTAACC CTCAGTCCAC CGCCGATGCT 
TGGCAGGCGA AGGGGAGCAG ACTGCTGGAC GTATTCAGTA GCAAAAGGAA GTTGGTTATG 
CTGTGGGATC CAACTGAATC AGATGTAAAC GTGATGCTGG AGCTGCTGCT AAGTTATTCC 
AGAGCGCACG TGTACCACAA GCTAGCGCTG GGGCTGTTAA TTGGGTTGAA TGTGCCAAAG 
GCGCTACAAA ACCGCGTAGC GTACAGCGTC GTGGCGGCGT TTCGCAAAAA TCAATCTCCG 
GTCAAAGCAA ACTCCGACGC TACGGAGCAG AGCGAATATG AAAACTACCA GGAGCGTTGC 
CTGTTTGTGC TGCTGCAGCT GCGATTGCAC GCACTCGACC AGCCGCTGCC CATCGTGCAG 
CAGATCTTGC AAGATCCCTC CAGCGTGGAG GTGCGCAATG TGCCCGCTCT CGAGCAGCTA 
GCGGACGTGA AGGCAGCGGT TGACGAACGG TGCCCGGTTG CGTGTCTGGC CGCGATGCTT 
ACCACCACCA TCGGACACTG GGTGCCCGTC TTCTGCCAGG ACGGCGTGAA CTTGTTGCAG 
CTGCTGCTAG ATCACCACCT CGACACGGTT GTGGTGCGCT GCCTGGAGCT GATCAGTCTG 
CTGTTTACCG AGTGTTCCCA TGCGCTGCTG GGTAGCGAGC GATTCGCCGA CCTGCTGACA 
CAGCTGGTGC AGGATGACGT TGAGCTAGTT TGGACGCCGC CGAAGCCTTC CACCAGCACG 
ATCGATCCGC AACAGAGCGA ACAGATGCTC GCGCTGAAGC TGCTTGATCA GGCGAAGGTG 
GGCAAAGTGG GCACGATGCT GGTGTCGCAA GCGGTTTCGC ACTGGCGCTG TGGCTACGCA 
ACGCCGGCGG CGTTAGTCGG CATGTGGTGC GACTGGTTGA CGCAAATATC CAGCTGGACG 
AACAGTGCCA AGGTGCTGCG TTTGCTGAAC GTGCTCGCCT CGGTTTCGTT CGGCCACGCG 
GAAGCCTGGA AGGCGTTGCG CGATAAGCTG GCTCCATTTT TCAAGTCCTT CGCGCAGGTG 
AAACAAAAGC AACCGGGCAT ACACTCGCTC TGGTCTAAGA TAATCGGTAC CGAGCCGCCG 
CTGCTGTACG GCACCCTGCC AAACGATTGC GTGGCCCTGG CGCTGCTTGT GTTCGAGCTC 
GAGCACCGAC AGCTCGAACT GGACACCGAA CTCTGGCCCA AGCTGCTGCG TGCACTCAAG 
CGGAAGGAAT CGCTCAAGCT GGACGCGGCA CTGCGCGACG TGAGCGGAGG CCTGCGCACG 
CGCGAGGAAG ACTTTTGTCC TGCGGCCGAT TCGCTGGTCT ACTGCAAGCT GGCCCGCTTT 
CTGGTGCGCG TCAGCGTGGA GCATCCACTG TGTCTGGCCG TCTGGCAGCA GTTTTTCGCC 
GCCCTGCTGA CGCGCGTCAC GGACGTGGGC GACCCGGTGC ACGGTGTGTC GGATCGACTG 
TACGGCGCGG ACACCGGTCT GATGGAAAAG GTGAAACAGA CGCTGTGCCG TCTCGAGCTG 
CACTACCATA GCGCGCAAGA GACGAACAAA CCCGCGGTGG AAATGTTGCG TCTCGTCAAA 
GCGTTCCAGC TGTGGTTGCT GGACCCGGAC CTGAATCGCA TCGCGATGGA CGATCCGATC 
AATCTACCAG TGCAGTACGC CGTTCCCCAT CTGCAGGCGA CTCTGCAGGG CGTGCAGGAA 
TGTTGGCCCG AGTGCGTCAA CCAGCCGGCC CTCGTCACGC TGCACCGTCT GATGGTGGAG 
CGCTGGTACG CGCTTTACCG CGTCCATCCG GCCCAACCGC TAAAGGCCCA ACCGTCGGAA 
GGGACTGCTG CCGGCAAACC GGCGCTTACG CCAGTCGAGG CCATCCAGCG CCGGCTAGCA 
AACGCGTACA CCGATCCACT CCCAGTGCCG GTGGTCGAGG TGCGCGGCGA GCAGCAGGAT 
TTGCGCGACG CACTGGCGAA ACCGGCGGCG GTACGAGTCA GGCGCATCAC CGAGAGCCTG 
CGGATCGTGC GGCAGCACAT CGACAAAACG TACTGGACGA CGAAAGCCGA GCTGGAGCAG 
CGCCGGGACG AGCTGTTCGA GCTGTACCGG CAGCTGTACG TCAACGAGGA CAGGCAGCTG 
GTGAAGCAGG CCAAGTGTAG CGTGCTGTAC TGTGCCGGTG CCGCCACGGT CAAGGTGCAC 
ACGAAGTGTG CCACGCTGGA CAAGACCGTG CAGGAGCGCA TCGACGCACG GTTGCGCGGG 
CTGGAAGCGT TCCTGCAGAT GGCGATCAAC ATTCCACCGT ACATCGTCCA CCACACCATC 
TTGCTGCGCG AGCTGTGGAA CAGTCTGTTC GTCGACTACT GCGCCGAGAC GGACCAGACC 
GCGGTCCAGT CGCTGAATGC CATCATCCGG ACGATGCTGC GCACGCTGCT GCACGAGGTG 
TCCGATGCGA ACTTCGTGCC GCCGCTTACA TTCGCCGTTC GGCTAAGCCT CGAGACGTAC 
CGGAGCGAAC TGTCGAAGCT TATGTTCGAG GAGGTCGGGC AGCTGTTTGC GGACGCGCTC 
GAGACAGGAC ACAAACCGTC CAGCGTGATC GTTGCCCTGC TAGAGGACGG GCGCATTCCG 
TGCCGGCCGC TGCTGCAGGT GTACGGCCAG CTGGTGAAGC AGAAAACCGG CAGCCTGGAG 
CGGACCCTGT TTGTGAACAT GTTCGGCAGC AAGCTCGACC TGTCGCGGTG GCTAAAGGAG 
CATGACGTCG CGCCGGACGA GCTCGACCAG TTTGCCAAGC TGATAGTGAT CGGACTGTAC 
AAAGCGCGGC CCGCGGATAC CGTCAGCCCG GTGCGATCGG ACGAGCAAGC CGAGCAGGAT 
GAAAAGTTTG CAGACATCCT AATTTCGCAC CTGGTAACGT TGGCGAGCAG CAAATTTCCC 
GACAACTTTG GCAAACTGCT GCAGCACACG ATCAACGCGT ACTCGCAATG GCCCACGCTG 
CCGCCGGTGA TGCTGCTACG GTTGCTGAAC GTGCTGCGTT CGCGGGCCCA CCATCTGCCC 
GATCTGTGCC TGGGCATGGC GGAGGACGCA CTGCACCAGG CCCATCGCCA GTTTGCCGAA 
GCGAACTGCA CCGAGCCGGT GCTGGGGCCG GAACTGTTGC ACCATCTGCT GGTGGCCGTG 
ACCGAGCACT TTGTGCGCCA GCGCGATGGC GCTTACCCAT GGAACGGCAT GTACAGGCGG 
CACGGCGCGT ACATCGAGGT GCTGGGAATG CTGCTGGGCA TGTTTGCCCA CAGCCTGCTT 
GCCGCCGGCA TGCGTGACAA TCGCATCGGA TGCGTGCACG ACAACCTGCT GCCCACCGTG 
TATCGCATGT TTGAGCCGTG GATCGTACCG TACGGTGCGG ACGTCCATGC ACACGCCCGG 
CCCGGTGGAT ACTCGCCGTC CACACCGTAC GGCAGGACGA CCATGGCGCC CGTGATCGAC 
ACAGCGCGAC ACTGCAACAA CGATAAGGCC AAGTGGATGT TCGGCGTGCT GCTGCACTGC 
GTCGAGTACG CAATCGAGCA GGTGCGCGAA GCGTACGCCG ACACCGACCA TTGCAGCCAG 
ATACTGCTGT ACTTCCTTCA CTGGTACCTG GAATGGTTCG TCGATCCGCG CATCATGATT 
TCGGCCCTGT ACGTGTACAA CACGCTCGTG CTCGATCTGC CCTGGGAGCG GCTGCTGCCG 
TCGGAGCTGC TGCTCGAGCG TATGCACGCC ATGCTGGAGC ACCACAGCCC CGAGTGCCAC 
GAGCTGCTGG CGTGCGTGTT CGTGCGTTGC CGCTGGTTCG GCGAACCGTT CGATCCCCTG 
CCCGGCTGGA TACGGCGCAC GCACGCCCCT ACGCTCGCGA TCTGCGTACG GCTAGCGTAC 
GAGCCGGTGG TACGGTCGGA GGAGCAAACG CGCACGGCCA TGGTGCGGCT GCTGCACCAC 
TTTGCCCGCC TCGTGTGGAT GCCGATGCAC GTTGCCGAGC TGACGCCAGC ACTGGACTGG 
TTCGTAATGA CCGCGGAAGC AGGCTTTCTG CTGCGCGAGC CGAAGGTGCC GCACCGCGAC 
CTGGACGATG CGCTCATGCT CTTGCTTGAA ATCGTGACGG GAATGCGGTG CAACGAAGCG 
AACCAGCTCG TGCCGGGCGG CATGTATCTG TTGAAGCGCA AGCTGTACAT CGGCGTCGTC 
GTGCGGATGC TCATGAATGC CGGCCGGGCG GCAAGCAAGG TGGCGAACGG GAAGCCCCAG 
CTGACCGCGG CCGTACAGCG GCTGGTTGCG TCGATAGCGG ATGTACTGGC ACAATTGCCG 
GACGCGTGCG GCACCGGCTG CTCGCACTGT CCCCGGCTGC TCGAGGCGCG CGCCTTGATG 
ACTGAGCTGC TGACCAGTGT CAAAAAGTGG CAAACCGAAA ATACGCTAAG GTACAGTTCT 
TTACCCATCC TAACCTCATT TACCCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Anopheles gambiae genome: an update.
Trends in parasitology20(2)49-52(2004 Feb)
Mongin E,Louis C,Holt RA,Birney E,Collins FH

The genome sequence of the malaria mosquito Anopheles gambiae.
Science (New York, N.Y.)298(5591)129-49(2002 Oct)
Holt RA,Subramanian GM,Halpern A,Sutton GG,Charlab R,Nusskern DR,Wincker P,Clark AG,Ribeiro JM,Wides R,Salzberg SL,Loftus B,Yandell M,Majoros WH,Rusch DB,Lai Z,Kraft CL,Abril JF,Anthouard V,Arensburger P,Atkinson PW,Baden H,de Berardinis V,Baldwin D,Benes V,Biedler J,Blass C,Bolanos R,Boscus D,Barnstead M,Cai S,Center A,Chaturverdi K,Christophides GK,Chrystal MA,Clamp M,Cravchik A,Curwen V,Dana A,Delcher A,Dew I,Evans CA,Flanigan M,Grundschober-Freimoser A,Friedli L,Gu Z,Guan P,Guigo R,Hillenmeyer ME,Hladun SL,Hogan JR,Hong YS,Hoover J,Jaillon O,Ke Z,Kodira C,Kokoza E,Koutsos A,Letunic I,Levitsky A,Liang Y,Lin JJ,Lobo NF,Lopez JR,Malek JA,McIntosh TC,Meister S,Miller J,Mobarry C,Mongin E,Murphy SD,O'Brochta DA,Pfannkoch C,Qi R,Regier MA,Remington K,Shao H,Sharakhova MV,Sitter CD,Shetty J,Smith TJ,Strong R,Sun J,Thomasova D,Ton LQ,Topalis P,Tu Z,Unger MF,Walenz B,Wang A,Wang J,Wang M,Wang X,Woodford KJ,Wortman JR,Wu M,Yao A,Zdobnov EM,Zhang H,Zhao Q,Zhao S,Zhu SC,Zhimulev I,Coluzzi M,della Torre A,Roth CW,Louis C,Kalush F,Mural RJ,Myers EW,Adams MD,Smith HO,Broder S,Gardner MJ,Fraser CM,Birney E,Bork P,Brey PT,Venter JC,Weissenbach J,Kafatos FC,Collins FH,Hoffman SL

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AgaP_AGAP000020 cDNA ORF clone, Anopheles gambiae str. PEST

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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