glis2 cDNA ORF clone, Anolis carolinensis(green anole)

Gene
Clones
gRNAs

Summary

Gene Symbol	glis2
Entrez Gene ID	103281514
Full Name	GLIS family zinc finger 2
Gene Type	protein-coding
Organism	Anolis carolinensis(green anole)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008123232.2	XP_008121439.1	zinc finger protein GLIS2

Pathways from BioSystems
Gene Ontology

The following glis2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the glis2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAn20219	XM_008123232.2 Latest version!	Anolis carolinensis GLIS family zinc finger 2 (glis2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

glis2 ( XM_008123232.2 ) cDNA ORF clone, Anolis carolinensis(green anole) -> XP_008121439.1 Anolis carolinensis GLIS family zinc finger 2 (glis2), mRNA.

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CloneID	OAn20219
Clone ID Related Accession (Same CDS sequence)	XM_008123232.2
Accession Version	XM_008123232.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-30
Organism	Anolis carolinensis(green anole)
Product	zinc finger protein GLIS2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003339403.1) and transcript sequence (GAFD01016176.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 31, 2016 this sequence version replaced XM_008123232.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Anolis carolinensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1542 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCATTCCC TGGACGAGCC CCTGGACCTC AAGCTGAGCG TTTCCAGGCT CCGGGAGAAG 
GGCGGCTGGG CCCCCGTTGG CCCCAAGCGC CTGGACCCCC GGGCCTCAGA GGAGGAGGAA 
GAAGAGGATG AAGAGGAGGA GGGGACCCCC ATCGCCAGCC CGCCCTCCCC CTGCTCCCCC 
AATAGTGGCC TCCCACTGCG GCCCCGGTGC CCCCAGGACA AGAGGGAAGC CCCCTGCCCC 
TCGCAGCCGC CAACCATTGA CCTCCGCCTC TCACCGCCCC CTCCATCCTC GGAGACCCCC 
GCAACATCCT CCTCCTTCTC TGCTTTGCGG GACCTACCCT CGCTGCGCTA CATTGAGGCC 
CTGCAGAACT CCTTCCAGTT CTTCCTGCCC CTGAATGCAG GGGGGCTGCC CCTGCCCGCC 
TCGGCATTCC TGGGCCCCCC CAAGGAGAAG CGCCTCTCTC CGGAGCTCCC TCTGCCGAAG 
CAGCAGCTGG TCTGCCAGTG GTCCAAGTGC CACCGGCCCT TTGACCTCCT GCAAGACCTA 
GTAGACCACG TCAACGACTT CCATGTCAAG CCCGAGAAGG ACACTGGGTA CTGCTGCCAC 
TGGGAGGGAT GTGCCCGCCA CGGAAGGGGC TTCAACGCCA GGTACAAGAT GCTGATCCAC 
ATCCGGACCC ACACCAACGA GAAGCCCCAC CGCTGCCCCA CCTGCAACAA GAGCTTCTCC 
CGTCTGGAGA ACCTGAAGAT CCACAACCGT TCACATACAG GGGAGAAACC TTACTTGTGC 
CCCTATGAAG GCTGCAACAA GCGCTACTCC AACTCCAGCG ACCGCTTCAA GCACACGCGC 
ACACACTACG TGGACAAGCC CTACTACTGC AAGATGCCCG GCTGCCACAA GCGCTACACG 
GACCCCAGCT CCCTGCGCAA GCACATCAAG GCCCACGGGC ACCACATCTC CCAGGAGCAG 
CAGCAGGAGA TACTCCAGGC CCAAGGGAGC CCCTCTGCCA TCATCACTGC CTCCGCTGCC 
TCCAAGGGGG GCTCCCTGGC GGGGGACGGG CCTTTCGCTG GCGCCCAGCT CCTCCTCCCC 
GGCCTCTTTG GGGGCCCTGG CTTGAGTGGC CTGCCCCTTG GATCCCCAGG CCCCCTGGAC 
CTCAGCGCCT TGGCCTGCGC TGGACTGGGC TCCACAGCAG CCATCGCTGG CTTGCCCAGT 
CCGGTCCTGG CCCCCCTGAA CCTGGCCAAG AACCCCCTTC TCTCCACCCC CTTTGCACCC 
GGCGGACTGG GGCTGCCCTT GGTCTCCCTG CTGGCCGGAG CCTCCATCGC CAAGGCTGAG 
AGGTGCCCCC ATGGGAACGG AGGCCGGCTC CTTAAAGGCG GTGGCAGTGG TGGCATTGTC 
AGGGGCAGCT GCAAAGGGCT TCTGGAGCGG ACTGACTTGG CCCGGCTGCG ACCCGGGTCC 
CCTGTGCCCG AGGAGGGTCT GCCCTTGCTC CCTGGTAGCG CCTTGGACCT CTCCACTGGG 
GTCAACTCCG GGGGGAACCG GAATAGCAGC AGCGGAGGCG AGGCCTTGCC CCCCGGATGG 
GTGGTCATCC CCACAGGATC GGTGTTGCTC AAACAGGCAG CGGTGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008121439.1
CDS	163..1773
Translation	MHSLDEPLDLKLSVSRLREKGGWAPVGPKRLDPRASEEEEEEDEEEEGTPIASPPSPCSPNSGLPLRPRCPQDKREAPCPSQPPTIDLRLSPPPPSSETPATSSSFSALRDLPSLRYIEALQNSFQFFLPLNAGGLPLPASAFLGPPKEKRLSPELPLPKQQLVCQWSKCHRPFDLLQDLVDHVNDFHVKPEKDTGYCCHWEGCARHGRGFNARYKMLIHIRTHTNEKPHRCPTCNKSFSRLENLKIHNRSHTGEKPYLCPYEGCNKRYSNSSDRFKHTRTHYVDKPYYCKMPGCHKRYTDPSSLRKHIKAHGHHISQEQQQEILQAQGSPSAIITASAASKGGSLAGDGPFAGAQLLLPGLFGGPGLSGLPLGSPGPLDLSALACAGLGSTAAIAGLPSPVLAPLNLAKNPLLSTPFAPGGLGLPLVSLLAGASIAKAERCPHGNGGRLLKGGGSGGIVRGSCKGLLERTDLARLRPGSPVPEEGLPLLPGSALDLSTGVNSGGNRNSSSGGEALPPGWVVIPTGSVLLKQAAVN

Target ORF information:

RefSeq Version	XM_008123232.2
Organism	Anolis carolinensis(green anole)
Definition	Anolis carolinensis GLIS family zinc finger 2 (glis2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008123232.2

ORF Insert Sequence:

ATGCATTCCC TGGACGAGCC CCTGGACCTC AAGCTGAGCG TTTCCAGGCT CCGGGAGAAG 
GGCGGCTGGG CCCCCGTTGG CCCCAAGCGC CTGGACCCCC GGGCCTCAGA GGAGGAGGAA 
GAAGAGGATG AAGAGGAGGA GGGGACCCCC ATCGCCAGCC CGCCCTCCCC CTGCTCCCCC 
AATAGTGGCC TCCCACTGCG GCCCCGGTGC CCCCAGGACA AGAGGGAAGC CCCCTGCCCC 
TCGCAGCCGC CAACCATTGA CCTCCGCCTC TCACCGCCCC CTCCATCCTC GGAGACCCCC 
GCAACATCCT CCTCCTTCTC TGCTTTGCGG GACCTACCCT CGCTGCGCTA CATTGAGGCC 
CTGCAGAACT CCTTCCAGTT CTTCCTGCCC CTGAATGCAG GGGGGCTGCC CCTGCCCGCC 
TCGGCATTCC TGGGCCCCCC CAAGGAGAAG CGCCTCTCTC CGGAGCTCCC TCTGCCGAAG 
CAGCAGCTGG TCTGCCAGTG GTCCAAGTGC CACCGGCCCT TTGACCTCCT GCAAGACCTA 
GTAGACCACG TCAACGACTT CCATGTCAAG CCCGAGAAGG ACACTGGGTA CTGCTGCCAC 
TGGGAGGGAT GTGCCCGCCA CGGAAGGGGC TTCAACGCCA GGTACAAGAT GCTGATCCAC 
ATCCGGACCC ACACCAACGA GAAGCCCCAC CGCTGCCCCA CCTGCAACAA GAGCTTCTCC 
CGTCTGGAGA ACCTGAAGAT CCACAACCGT TCACATACAG GGGAGAAACC TTACTTGTGC 
CCCTATGAAG GCTGCAACAA GCGCTACTCC AACTCCAGCG ACCGCTTCAA GCACACGCGC 
ACACACTACG TGGACAAGCC CTACTACTGC AAGATGCCCG GCTGCCACAA GCGCTACACG 
GACCCCAGCT CCCTGCGCAA GCACATCAAG GCCCACGGGC ACCACATCTC CCAGGAGCAG 
CAGCAGGAGA TACTCCAGGC CCAAGGGAGC CCCTCTGCCA TCATCACTGC CTCCGCTGCC 
TCCAAGGGGG GCTCCCTGGC GGGGGACGGG CCTTTCGCTG GCGCCCAGCT CCTCCTCCCC 
GGCCTCTTTG GGGGCCCTGG CTTGAGTGGC CTGCCCCTTG GATCCCCAGG CCCCCTGGAC 
CTCAGCGCCT TGGCCTGCGC TGGACTGGGC TCCACAGCAG CCATCGCTGG CTTGCCCAGT 
CCGGTCCTGG CCCCCCTGAA CCTGGCCAAG AACCCCCTTC TCTCCACCCC CTTTGCACCC 
GGCGGACTGG GGCTGCCCTT GGTCTCCCTG CTGGCCGGAG CCTCCATCGC CAAGGCTGAG 
AGGTGCCCCC ATGGGAACGG AGGCCGGCTC CTTAAAGGCG GTGGCAGTGG TGGCATTGTC 
AGGGGCAGCT GCAAAGGGCT TCTGGAGCGG ACTGACTTGG CCCGGCTGCG ACCCGGGTCC 
CCTGTGCCCG AGGAGGGTCT GCCCTTGCTC CCTGGTAGCG CCTTGGACCT CTCCACTGGG 
GTCAACTCCG GGGGGAACCG GAATAGCAGC AGCGGAGGCG AGGCCTTGCC CCCCGGATGG 
GTGGTCATCC CCACAGGATC GGTGTTGCTC AAACAGGCAG CGGTGAACTG A