klhl12 cDNA ORF clone, Anolis carolinensis(green anole)

Gene
Clones
gRNAs

Summary

Gene Symbol	klhl12
Entrez Gene ID	100561958
Full Name	kelch like family member 12
Gene Type	protein-coding
Organism	Anolis carolinensis(green anole)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003220465.3	XP_003220513.1	kelch-like protein 12

Pathways from BioSystems
Gene Ontology

The following klhl12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the klhl12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAn34379	XM_003220465.3 Latest version!	Anolis carolinensis kelch like family member 12 (klhl12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

klhl12 ( XM_003220465.3 ) cDNA ORF clone, Anolis carolinensis(green anole) -> XP_003220513.1 Anolis carolinensis kelch like family member 12 (klhl12), transcript variant X1, mRNA.

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CloneID	OAn34379
Clone ID Related Accession (Same CDS sequence)	XM_003220465.3
Accession Version	XM_003220465.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-30
Organism	Anolis carolinensis(green anole)
Product	kelch-like protein 12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_014779.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 31, 2016 this sequence version replaced XM_003220465.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Anolis carolinensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCCTA AAGATATCAT GACTAATTCT CATGCCAAAT CCATTCTCAA TGCAATGAAT 
TCTCTGCGAA AAAGTAACAC ATTATGTGAT GTGACCCTTC GTGTGGAACA AAAATACTTT 
CCAGCCCATC GGATTGTGCT AGCTGCTTGC AGTGACTACT TCTGTGCTAT GTTCACCAGT 
GAGCTTTCAG AGAAGGATAA ACCCTATGTA GATATCCAAG GTCTAACAGC CTCAACAATG 
GAAATACTGC TGGACTTTGT GTACACAGAA ACTGTTCATG TCACAGTGGA GAATGTTCAA 
GAGTTGCTCC CAGCAGCCTG TCTACTTCAG CTAAAAGGAG TAAAGCAGGC TTGTTGTGAA 
TTCTTGGAAA GCCAACTGGA TCCATCCAAC TGTCTGGGCA TCCGAGATTT TGCTGAAACA 
CACAATTGTG TTGACTTAAT GCAGGCAGCT GAAGTCTTCA GCCAGAAGCA CTTCCCAGAT 
GTAGTTCAGC ATGAGGAGTT TATACTTCTG AATCAAGAAG AAGTGGAAAA GCTCATCAAA 
TGTGATGAAA TACAGGTTGA TTCTGAAGAG CCTGTCTTTG AGGCAGTTAT TAATTGGGTG 
AAGCACTCAA AGAAAGAACG GGAAGCATCC CTGCCGGAGC TGCTACAATA TGTCCGCATG 
CCACTTCTCA CCCCACGCTA CATCACTGAC GTCATAGACA CTGAGCCTTT TATTAGATGC 
AGTTTGCAAT GCAGAGACCT TGTAGATGAA GCTAAGAAGT TTCATTTAAG GCCTGAGCTT 
CGAAGCCAAA TGCAGGGACC TAGAACCAGA GCACGTTTAG GTGCCAATGA GGTGCTTTTA 
GTAATTGGAG GTTTTGGAAG TCAGCAGTCA CCTATTGATG TGGTGGAGAA ATATGATCCA 
AAAACACAGG AATGGAGCTT CTTACCCAGC ATCAGCCGTA AAAGACGCTA TGTTGCCACA 
GTTTCCTTGC ATGATCGGAT ATATGTAATC GGTGGTTACG ATGGCCGTTC TCGTCTTAGC 
TCCGTAGAAT GTTTGGATTA TACATCTGAT GAAGACAGTA TCTGGTACTC TGTGGCTCCT 
ATGAATGTTC GACGGGGCCT CGCAGGGGCC ACTACTTTGG GAGACATGAT CTATGTTTCT 
GGAGGTTTTG ATGGAAGCCG GCGTCACACC AGTATGGAAA GATATGATCC CAACATTGAT 
CAGTGGAGCA TGCTGGGAGA TATGCAGACA GCTCGAGAAG GAGCAGGGCT GGTGGTTGCT 
AATAATGTTA TCTACTGTCT TGGTGGCTAT GATGGTCTAA ACATCTTAAA TTCTGTGGAG 
CGATATGATC CTCACACGGG ACATTGGACA AATGTCACTC CGATGGCCAC CAAACGTTCA 
GGAGCTGGAG TAGCCTTGTT GAATGATCAT ATTTATGTGG TTGGGGGTTT CGATGGAACA 
GCACATCTGT CCTCTGTAGA AGCATATAAC ATTCGCACTG ACTCCTGGAC AACAGTGACC 
AGCATGACTA CTCCTCGCTG TTATGTGGGT GCAACAGTGC TGAGGGGAAG GCTTTATGCC 
ATAGCAGGGT ATGACGGGAA CTCCCTCCTG AGCAGCATTG AGTGTTACGA CCCTATTATT 
GACAGCTGGG AAGTGGTAAC ATCACTGGGG ACCCAACGTT GTGATGCTGG TGTTTGTGTG 
CTGCGGGAAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003220513.1
CDS	338..2032
Translation	MAPKDIMTNSHAKSILNAMNSLRKSNTLCDVTLRVEQKYFPAHRIVLAACSDYFCAMFTSELSEKDKPYVDIQGLTASTMEILLDFVYTETVHVTVENVQELLPAACLLQLKGVKQACCEFLESQLDPSNCLGIRDFAETHNCVDLMQAAEVFSQKHFPDVVQHEEFILLNQEEVEKLIKCDEIQVDSEEPVFEAVINWVKHSKKEREASLPELLQYVRMPLLTPRYITDVIDTEPFIRCSLQCRDLVDEAKKFHLRPELRSQMQGPRTRARLGANEVLLVIGGFGSQQSPIDVVEKYDPKTQEWSFLPSISRKRRYVATVSLHDRIYVIGGYDGRSRLSSVECLDYTSDEDSIWYSVAPMNVRRGLAGATTLGDMIYVSGGFDGSRRHTSMERYDPNIDQWSMLGDMQTAREGAGLVVANNVIYCLGGYDGLNILNSVERYDPHTGHWTNVTPMATKRSGAGVALLNDHIYVVGGFDGTAHLSSVEAYNIRTDSWTTVTSMTTPRCYVGATVLRGRLYAIAGYDGNSLLSSIECYDPIIDSWEVVTSLGTQRCDAGVCVLREK

Target ORF information:

RefSeq Version	XM_003220465.3
Organism	Anolis carolinensis(green anole)
Definition	Anolis carolinensis kelch like family member 12 (klhl12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003220465.3

ORF Insert Sequence:

ATGGCTCCTA AAGATATCAT GACTAATTCT CATGCCAAAT CCATTCTCAA TGCAATGAAT 
TCTCTGCGAA AAAGTAACAC ATTATGTGAT GTGACCCTTC GTGTGGAACA AAAATACTTT 
CCAGCCCATC GGATTGTGCT AGCTGCTTGC AGTGACTACT TCTGTGCTAT GTTCACCAGT 
GAGCTTTCAG AGAAGGATAA ACCCTATGTA GATATCCAAG GTCTAACAGC CTCAACAATG 
GAAATACTGC TGGACTTTGT GTACACAGAA ACTGTTCATG TCACAGTGGA GAATGTTCAA 
GAGTTGCTCC CAGCAGCCTG TCTACTTCAG CTAAAAGGAG TAAAGCAGGC TTGTTGTGAA 
TTCTTGGAAA GCCAACTGGA TCCATCCAAC TGTCTGGGCA TCCGAGATTT TGCTGAAACA 
CACAATTGTG TTGACTTAAT GCAGGCAGCT GAAGTCTTCA GCCAGAAGCA CTTCCCAGAT 
GTAGTTCAGC ATGAGGAGTT TATACTTCTG AATCAAGAAG AAGTGGAAAA GCTCATCAAA 
TGTGATGAAA TACAGGTTGA TTCTGAAGAG CCTGTCTTTG AGGCAGTTAT TAATTGGGTG 
AAGCACTCAA AGAAAGAACG GGAAGCATCC CTGCCGGAGC TGCTACAATA TGTCCGCATG 
CCACTTCTCA CCCCACGCTA CATCACTGAC GTCATAGACA CTGAGCCTTT TATTAGATGC 
AGTTTGCAAT GCAGAGACCT TGTAGATGAA GCTAAGAAGT TTCATTTAAG GCCTGAGCTT 
CGAAGCCAAA TGCAGGGACC TAGAACCAGA GCACGTTTAG GTGCCAATGA GGTGCTTTTA 
GTAATTGGAG GTTTTGGAAG TCAGCAGTCA CCTATTGATG TGGTGGAGAA ATATGATCCA 
AAAACACAGG AATGGAGCTT CTTACCCAGC ATCAGCCGTA AAAGACGCTA TGTTGCCACA 
GTTTCCTTGC ATGATCGGAT ATATGTAATC GGTGGTTACG ATGGCCGTTC TCGTCTTAGC 
TCCGTAGAAT GTTTGGATTA TACATCTGAT GAAGACAGTA TCTGGTACTC TGTGGCTCCT 
ATGAATGTTC GACGGGGCCT CGCAGGGGCC ACTACTTTGG GAGACATGAT CTATGTTTCT 
GGAGGTTTTG ATGGAAGCCG GCGTCACACC AGTATGGAAA GATATGATCC CAACATTGAT 
CAGTGGAGCA TGCTGGGAGA TATGCAGACA GCTCGAGAAG GAGCAGGGCT GGTGGTTGCT 
AATAATGTTA TCTACTGTCT TGGTGGCTAT GATGGTCTAA ACATCTTAAA TTCTGTGGAG 
CGATATGATC CTCACACGGG ACATTGGACA AATGTCACTC CGATGGCCAC CAAACGTTCA 
GGAGCTGGAG TAGCCTTGTT GAATGATCAT ATTTATGTGG TTGGGGGTTT CGATGGAACA 
GCACATCTGT CCTCTGTAGA AGCATATAAC ATTCGCACTG ACTCCTGGAC AACAGTGACC 
AGCATGACTA CTCCTCGCTG TTATGTGGGT GCAACAGTGC TGAGGGGAAG GCTTTATGCC 
ATAGCAGGGT ATGACGGGAA CTCCCTCCTG AGCAGCATTG AGTGTTACGA CCCTATTATT 
GACAGCTGGG AAGTGGTAAC ATCACTGGGG ACCCAACGTT GTGATGCTGG TGTTTGTGTG 
CTGCGGGAAA AGTGA