fem1b cDNA ORF clone, Anolis carolinensis(green anole)

Gene
Clones
gRNAs

Summary

Gene Symbol	fem1b
Entrez Gene ID	100560992
Full Name	fem-1 homolog B
Gene Type	protein-coding
Organism	Anolis carolinensis(green anole)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003227245.3	XP_003227293.1	protein fem-1 homolog B

Pathways from BioSystems
Gene Ontology

The following fem1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fem1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAn16945	XM_003227245.3 Latest version!	Anolis carolinensis fem-1 homolog B (fem1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fem1b ( XM_003227245.3 ) cDNA ORF clone, Anolis carolinensis(green anole) -> XP_003227293.1 Anolis carolinensis fem-1 homolog B (fem1b), mRNA.

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CloneID	OAn16945
Clone ID Related Accession (Same CDS sequence)	XM_003227245.3
Accession Version	XM_003227245.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-30
Organism	Anolis carolinensis(green anole)
Product	protein fem-1 homolog B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003339014.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 31, 2016 this sequence version replaced XM_003227245.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Anolis carolinensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGCC TGGCCGGCTA CGTCTACAAG GCGGCCAGTG AGGGGCGGGT GCTGACGCTG 
GCCGCCTTGC TCCTGAACCG TTCCGAGAGC GATGTCCGCT ACCTGCTGGG CTTCGTCACC 
CACCAAGGCG GGCAGCGCTC CACGCCGCTC ATCATCGCCG CCCGCAACGG ACACACCAAA 
GTGGTGCGGC TGCTCCTAGA ACACTACCAC GTCCAGACCC AGCAGACAGG CACCGTCCGG 
TTTGATGGCT ACGTCATCGA CGGTGCCACG GCACTGTGGT GCGCAGCTGG AGCCGGCCAC 
TTTGAAGTCG TGAAGCTCCT GGTGAGCCAT GGGGCCAACG TCAACCACAC TACAGTGACC 
AACTCCACTC CGCTGCGGGC CGCCTGCTTT GACGGCAGAC TTGACATCGT GAGGTACCTG 
GTAGAGAACC ATGCCAACAT CAGCATCGCC AACAAGTATG ATAACACTTG CCTTATGATC 
GCTGCGTACA AGGGGCACGT GGATGTGGTG CGCTACCTCC TGGAGCAGAA TGCTGACCCC 
AACGCCAAAG CTCACTGTGG CGCCACGGCC TTACATTTTG CTGCCGAGGC AGGCCACTTG 
GAGATCGTCC GCGAACTGGT GAAGTGGAAG TCCGCCATGA TGGTGAATGG CCACGGCATG 
ACGCCCTTGA AAGTGGCTGC TGAGAGCTGC AAGGCGGACG TGGTGGAGAT GCTGCTGGCT 
CATGCCGACT GTACCCGCAA GAGCCGGATC GAAGCTTTGG AGCTGCTGGG CGCCTCGTTC 
GCCAACGACA GAGAGAACTA CGACATAATG AAAACCTACC ACTATTTATA TTTAGCCATG 
CTGGAGCGAT ACAGGGACAG CGAGAACATT ATTGAGAAGG AGGTCCTGCC TCCGATAGAA 
GCCTATGGTG GCAGGACCGA GTGCCGGACT CCTCAGGAGC TGGAGGTCAT TCGGCAGGAC 
AGAGACGCCC TCCACATGGA GGGCCTCATT GTCCGGGAGC GGATTCTGGG CTCCGACAAC 
ATTGACGTCT CGCACCCCAT CATCTACCGC GGCGCCGTGT ACGCGGACAA CATGCAGTTT 
GAGCAGTGCA TCAAGCTCTG GCTCCACGCC TTGCATCTCC GGCAGAAGGG CAACAGGAAC 
ACTCACAAGG ACCTCCTCCG GTTCGCTCAG GTCTTCTCTC AGATGATCCA CTTGAATGAG 
CCGGTGAGGG CCAAGGACAT CGAGAGCGTC TTGAGGTGCA GCGTCTTGGA GATCGAGCAG 
GGCATGGCCC GGGTTAAAAG CTCCCAAGAG GCCGAGCTCC ACACCGCCCT GGACAACTAC 
GAGTGCAACA TCTTCACCTT CTTGTACTTG GTGTGCATCT CCACCAAGAC GCAGTGCCGG 
GAGGAGGAGC AAGCACGGAT CAACAAGCAG ATTTATACTT TGATCCAGCT GGACCCCCGG 
ACGCGAGACG GGTCCAGCTT GCTCCACCAC GCGGTCAACT CCGGTACGCC GGTGGACGAC 
TTCCACACCA ACGATGTCTG CAGCTTCCCC AACGCGCTGG TCACCAAGCT GCTCCTGGAC 
TGTGGGGCCA GCGTCAACGC AGCAGACCTC GAAGGCAACA CGCCGCTCCA CGTCATCGTC 
CAGTACAACC GGCCCATCAG TGACTTCTTG ACTTTGCACT CCATCATTAT CGGCCTGGTG 
GAGGCCGGGG CGCACACGGA CATGGCCAAC AAGCAGAAGA AGACGCCGCT GGACAAGAGC 
ACCACCGGCG TCTCCGAAAT CCTCCTGAAA ACTCAAATGA AGCTGAGTCT GAAGTGCCTG 
GCCGCCCGAG CCGTGCGGGC CCATCATATC AGCTATCGCA ACCAGATCCC CAAGACACTG 
GAAGAGTTTG TAGAGTTTCA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003227293.1
CDS	603..2486
Translation	MESLAGYVYKAASEGRVLTLAALLLNRSESDVRYLLGFVTHQGGQRSTPLIIAARNGHTKVVRLLLEHYHVQTQQTGTVRFDGYVIDGATALWCAAGAGHFEVVKLLVSHGANVNHTTVTNSTPLRAACFDGRLDIVRYLVENHANISIANKYDNTCLMIAAYKGHVDVVRYLLEQNADPNAKAHCGATALHFAAEAGHLEIVRELVKWKSAMMVNGHGMTPLKVAAESCKADVVEMLLAHADCTRKSRIEALELLGASFANDRENYDIMKTYHYLYLAMLERYRDSENIIEKEVLPPIEAYGGRTECRTPQELEVIRQDRDALHMEGLIVRERILGSDNIDVSHPIIYRGAVYADNMQFEQCIKLWLHALHLRQKGNRNTHKDLLRFAQVFSQMIHLNEPVRAKDIESVLRCSVLEIEQGMARVKSSQEAELHTALDNYECNIFTFLYLVCISTKTQCREEEQARINKQIYTLIQLDPRTRDGSSLLHHAVNSGTPVDDFHTNDVCSFPNALVTKLLLDCGASVNAADLEGNTPLHVIVQYNRPISDFLTLHSIIIGLVEAGAHTDMANKQKKTPLDKSTTGVSEILLKTQMKLSLKCLAARAVRAHHISYRNQIPKTLEEFVEFH

Target ORF information:

RefSeq Version	XM_003227245.3
Organism	Anolis carolinensis(green anole)
Definition	Anolis carolinensis fem-1 homolog B (fem1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003227245.3

ORF Insert Sequence:

ATGGAGAGCC TGGCCGGCTA CGTCTACAAG GCGGCCAGTG AGGGGCGGGT GCTGACGCTG 
GCCGCCTTGC TCCTGAACCG TTCCGAGAGC GATGTCCGCT ACCTGCTGGG CTTCGTCACC 
CACCAAGGCG GGCAGCGCTC CACGCCGCTC ATCATCGCCG CCCGCAACGG ACACACCAAA 
GTGGTGCGGC TGCTCCTAGA ACACTACCAC GTCCAGACCC AGCAGACAGG CACCGTCCGG 
TTTGATGGCT ACGTCATCGA CGGTGCCACG GCACTGTGGT GCGCAGCTGG AGCCGGCCAC 
TTTGAAGTCG TGAAGCTCCT GGTGAGCCAT GGGGCCAACG TCAACCACAC TACAGTGACC 
AACTCCACTC CGCTGCGGGC CGCCTGCTTT GACGGCAGAC TTGACATCGT GAGGTACCTG 
GTAGAGAACC ATGCCAACAT CAGCATCGCC AACAAGTATG ATAACACTTG CCTTATGATC 
GCTGCGTACA AGGGGCACGT GGATGTGGTG CGCTACCTCC TGGAGCAGAA TGCTGACCCC 
AACGCCAAAG CTCACTGTGG CGCCACGGCC TTACATTTTG CTGCCGAGGC AGGCCACTTG 
GAGATCGTCC GCGAACTGGT GAAGTGGAAG TCCGCCATGA TGGTGAATGG CCACGGCATG 
ACGCCCTTGA AAGTGGCTGC TGAGAGCTGC AAGGCGGACG TGGTGGAGAT GCTGCTGGCT 
CATGCCGACT GTACCCGCAA GAGCCGGATC GAAGCTTTGG AGCTGCTGGG CGCCTCGTTC 
GCCAACGACA GAGAGAACTA CGACATAATG AAAACCTACC ACTATTTATA TTTAGCCATG 
CTGGAGCGAT ACAGGGACAG CGAGAACATT ATTGAGAAGG AGGTCCTGCC TCCGATAGAA 
GCCTATGGTG GCAGGACCGA GTGCCGGACT CCTCAGGAGC TGGAGGTCAT TCGGCAGGAC 
AGAGACGCCC TCCACATGGA GGGCCTCATT GTCCGGGAGC GGATTCTGGG CTCCGACAAC 
ATTGACGTCT CGCACCCCAT CATCTACCGC GGCGCCGTGT ACGCGGACAA CATGCAGTTT 
GAGCAGTGCA TCAAGCTCTG GCTCCACGCC TTGCATCTCC GGCAGAAGGG CAACAGGAAC 
ACTCACAAGG ACCTCCTCCG GTTCGCTCAG GTCTTCTCTC AGATGATCCA CTTGAATGAG 
CCGGTGAGGG CCAAGGACAT CGAGAGCGTC TTGAGGTGCA GCGTCTTGGA GATCGAGCAG 
GGCATGGCCC GGGTTAAAAG CTCCCAAGAG GCCGAGCTCC ACACCGCCCT GGACAACTAC 
GAGTGCAACA TCTTCACCTT CTTGTACTTG GTGTGCATCT CCACCAAGAC GCAGTGCCGG 
GAGGAGGAGC AAGCACGGAT CAACAAGCAG ATTTATACTT TGATCCAGCT GGACCCCCGG 
ACGCGAGACG GGTCCAGCTT GCTCCACCAC GCGGTCAACT CCGGTACGCC GGTGGACGAC 
TTCCACACCA ACGATGTCTG CAGCTTCCCC AACGCGCTGG TCACCAAGCT GCTCCTGGAC 
TGTGGGGCCA GCGTCAACGC AGCAGACCTC GAAGGCAACA CGCCGCTCCA CGTCATCGTC 
CAGTACAACC GGCCCATCAG TGACTTCTTG ACTTTGCACT CCATCATTAT CGGCCTGGTG 
GAGGCCGGGG CGCACACGGA CATGGCCAAC AAGCAGAAGA AGACGCCGCT GGACAAGAGC 
ACCACCGGCG TCTCCGAAAT CCTCCTGAAA ACTCAAATGA AGCTGAGTCT GAAGTGCCTG 
GCCGCCCGAG CCGTGCGGGC CCATCATATC AGCTATCGCA ACCAGATCCC CAAGACACTG 
GAAGAGTTTG TAGAGTTTCA CTAG