DAGLA cDNA ORF clone, Alligator sinensis(Chinese alligator)

Gene
Clones
gRNAs

Summary

Gene Symbol	DAGLA
Entrez Gene ID	102388608
Full Name	diacylglycerol lipase alpha
Gene Type	protein-coding
Organism	Alligator sinensis(Chinese alligator)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025206945.1	XP_025062730.1	sn1-specific diacylglycerol lipase alpha
XM_014521047.1	XP_014376533.1	sn1-specific diacylglycerol lipase alpha

Pathways from BioSystems
Gene Ontology

The following DAGLA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DAGLA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAl20441	XM_014521047.1 Latest version!	Alligator sinensis diacylglycerol lipase, alpha (DAGLA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAl20441	XM_025206945.1 Latest version!	Alligator sinensis diacylglycerol lipase alpha (DAGLA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DAGLA ( XM_025206945.1 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_025062730.1 Alligator sinensis diacylglycerol lipase alpha (DAGLA), mRNA.

Price & Availability↑

CloneID	OAl20441
Clone ID Related Accession (Same CDS sequence)	XM_025206945.1 , XM_014521047.1
Accession Version	XM_025206945.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Alligator sinensis(Chinese alligator)
Product	sn1-specific diacylglycerol lipase alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005842176.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Alligator sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGGA TTGTGGTGTT CCGCCGTCGC TGGTCTGTAG GCAGCGATGA CCTCGTTTTG 
CCAGCCATCT TCCTTTTCCT CCTTCATACC ACCTGGTTTG TGATCCTTTC TGTGGTGCTC 
TTTGGGCTGG TGTACAATCC CAACGAGACC TGTTCCCTTA ACCTGGTGGA CCACGGCAGA 
GGCTACCTGG GCATCCTTCT CAGCTGCATG ATAGCAGAAG TGGCAATCAT CTGGCTCAGC 
ATGAGAGGCA GCATCCTGTA CACAGAGCCT CGGGATTCGA TGCAGTATGT GCTCTATGTC 
AGACTGGCCA TCCTGGTGAT CGAGTTTGTG TACGCTATAG TGGGCATTGT TTGGCTAACC 
CAGTACTACA CCTCGTGCAA TGACCTCACT GCCAAGAGTG TCACCTTGGG AATGGTAGTG 
TGCAACTGGG TCGTCATTCT GAGTGTCTGC ATCACTGTCC TGTGCGTCTT TGACCCGACA 
GGGCGAACCT TCGTCAAGCT TCGGGCCACA AAGAGAAGGC AGCGCAACCT GAGGACATAC 
AACCTGCGGC ACCGCCTGGA AGAGGGACAG GCCAGCAGCT GGACACGCCG CCTCAAAGTT 
TTTCTATGCT GCACGAGAAC AAAGGATTCC CAGTCAGATG CCTACTCTGA AATTGCCTAC 
CTGTTTGCTG AGTTTTTCCG AGACCTTGAC ATAGTCCCAT CTGACATCAT TGCAGGCCTG 
GTCCTCCTGC GGCAGCGGCA GAGGGCAAAA CGCAGCGCGG TGCTAGATGA GGCGAACAAT 
GACATTCTGG CCTTTCTGTC TGGGATGCCA GTGACAAGGA ACACCAAGTA CCTGGATCTG 
AAAAATGCAC AAGAGATGCA GCGGTACAAA GAAGTGTGTT ACTACATGCT CTTTGCCCTG 
GCTGCCTATG GCTGGCCCAT GTACCTGATG AGGAAACCCA CCTGTGGACT TTGTCGCCTG 
GCTAGATCCT GCTCATGTTG CTGTCTCTGT CCCTCACGGC CTCGCTATGC ACCTGGTGTC 
ACCATTGAGG AGGATAACTG CTGTGGGTGC AATGCTATTG CCATCCGTCG CCACTTTCTG 
GATGAAAACA TGACCTCTGT GGATATTGTC TACACCTCTT GTCATGATGC TGTCTACGAA 
ACGCCCTTCT ATGTGGCTGT GGACCATGAT AAGAAGAAAG TGGTCATCAG TATCCGAGGA 
ACTCTCTCTC CAAAGGATGC CCTGACTGAC TTAACTGGGG ATGCAGAACG CCTTCCAGTT 
GAGGGTCATC ATGGAACCTG GCTAGGACAC AAGGGAATGG TGTTGTCTGC TGAATACATC 
AAGAAGAAAC TGGAGCAGGA GATGGTTCTT TCTCAAGCCT TTGGGCATGA TTTAGGCAGA 
GGAACAAAGC ACTACGGCCT GATTGTGGTG GGACATTCCC TAGGGGCTGG CACAGCTGCC 
ATTCTGTCCT TCCTTCTGCG TCCCCAGTAC CCATCACTGA AGTGCTTTGC GTATTCTCCC 
CCAGGTGGGC TGCTGAGTGA GGATGCCATG GAGTACTCGA AAGAGTTTGT GACTGCAGTG 
GTGCTTGGCA AAGATCTAGT CCCCAGGATT GGTCTCTCTC AGCTGGAAGG ATTTCGCCGG 
CAGCTTCTTG ATGTTCTCCA GAGAAGTACC AAGCCTAAGT GGCGAATAAT TGTGGGTGCC 
ACAAAGTGTA TACCTAAGTC AGAGCTCCCT GAAGAGATAG AAGGGAATTC CGTGACCAGT 
AACCGTCTCT GGACCCACCC CAGCGACCTA ACCATTGCCC TGTCTGCCAG TACACCACTC 
TACCCACCCG GCCGCATCAT CCATGTGGTG CACAATCATC CTGTTGAGCA GTGCTGTTGC 
TGTGACCAGG AGGATCCCAC ATATTTTGCC ATTTGGGGGA ACAATAAAGC ATTTAATGAA 
GTGATCATCT CTCCAGCAAT GCTGCATGAG CACCTTCCCT ATGTGGTCAT GGAGGGACTC 
AACAAGGTCC TGGAGAACTA CAACAAGGGC AAGACTGCCT TACTTTCTGC AGCCAAAGTA 
ATGGTGAGTC CCACAGAAGT GGATCTCAAC CCAGAGTTGA TCTTCCAGAA TCAGCCCTTG 
CCCAGCGGGC CTGCAGTACA GGTTGGAACT GGAGCCATAT TAGCAGACAG AAGGAACAGC 
AGCATCAAGA GCAAATCACA ATCTGAAATC AGCTTGGAGG GGTTTTATGA GACCAAGCCC 
CTTTCTTCTG TGCAGAAGGA CCCTGTAGAG CTTCTGCTCC TGGATACCAA GGAGCGCCTG 
TCTGTGGAGC TGCAGGACCG CCGTGCTCCT CTGGCCACCA TGGAGAGTCT GTCCGATAAT 
GAGTCCATCT ACAGCTTTGA TTCACGGCGC TCCTCAGGCT TCCGGAGCAT CCGTGGCTCC 
CCCAGTCTCC ATGCTGTCAT GGAGAAGGAT GAGACGCACT GTTTTTATAT AGACCCCGTT 
ATCCCTGAGG AGAACCCCTC CCTCAGCTCG AGGACAGAGC TGCTTGCTGC TGACAGCCTC 
TCTAAACATT CACAGGAGAC ACAACCCCCT GAGACCTTGC ATAACAGTGG TGGCACTACC 
CCACAGCGAT GCTGCAGCGA GGAAGGGGCT GCCAGCGAAG GAGACAGAAT TTCCTTTACA 
GGCCGAGAGG AGCTGTCCCT CCAGAATGGA CGGCTCCCTG ATGTGCCCAG TCCACAAGTG 
CTGGAGTTTG CTGAGTTTAT AGACAGCCTC TTCAATCTGG ACAGCAAAAG CAGCTCCTTC 
CAGGACATCT ATTGCATGAT GGTATCAGAT AGCTCCAGTG ACTTTGCAGA GATGCCTAAG 
TCTGTCAGTG ATCAGGAGAT CCTGCTGAGA GCACAGTATG AGCCCAACCT GGTCCCCAAG 
CCCCCTAGGT TGTTCGCAGG CTCAACTGAT CCCTCTTCAG GCATCTCAGT CTCACCCTCC 
TTCCCTCTGA GTTCATCTGG AGAACTTATG GACATTACTC CCACAGGCAT GAGCAGCCAG 
GAATGCCTGG CCACTGACAA AATCCGGACT TCCACCCCGT CTGGTCACAT GACCAGCCCC 
ACCAAGCAGG AGGACCTCAT GATCTCAGCC CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025062730.1
CDS	175..3270
Translation	MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPNETCSLNLVDHGRGYLGILLSCMIAEVAIIWLSMRGSILYTEPRDSMQYVLYVRLAILVIEFVYAIVGIVWLTQYYTSCNDLTAKSVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQASSWTRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRSAVLDEANNDILAFLSGMPVTRNTKYLDLKNAQEMQRYKEVCYYMLFALAAYGWPMYLMRKPTCGLCRLARSCSCCCLCPSRPRYAPGVTIEEDNCCGCNAIAIRRHFLDENMTSVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGHDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPSLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGATKCIPKSELPEEIEGNSVTSNRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPVEQCCCCDQEDPTYFAIWGNNKAFNEVIISPAMLHEHLPYVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLNPELIFQNQPLPSGPAVQVGTGAILADRRNSSIKSKSQSEISLEGFYETKPLSSVQKDPVELLLLDTKERLSVELQDRRAPLATMESLSDNESIYSFDSRRSSGFRSIRGSPSLHAVMEKDETHCFYIDPVIPEENPSLSSRTELLAADSLSKHSQETQPPETLHNSGGTTPQRCCSEEGAASEGDRISFTGREELSLQNGRLPDVPSPQVLEFAEFIDSLFNLDSKSSSFQDIYCMMVSDSSSDFAEMPKSVSDQEILLRAQYEPNLVPKPPRLFAGSTDPSSGISVSPSFPLSSSGELMDITPTGMSSQECLATDKIRTSTPSGHMTSPTKQEDLMISAL

Target ORF information:

RefSeq Version	XM_025206945.1
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis diacylglycerol lipase alpha (DAGLA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025206945.1

ORF Insert Sequence:

ATGCCAGGGA TTGTGGTGTT CCGCCGTCGC TGGTCTGTAG GCAGCGATGA CCTCGTTTTG 
CCAGCCATCT TCCTTTTCCT CCTTCATACC ACCTGGTTTG TGATCCTTTC TGTGGTGCTC 
TTTGGGCTGG TGTACAATCC CAACGAGACC TGTTCCCTTA ACCTGGTGGA CCACGGCAGA 
GGCTACCTGG GCATCCTTCT CAGCTGCATG ATAGCAGAAG TGGCAATCAT CTGGCTCAGC 
ATGAGAGGCA GCATCCTGTA CACAGAGCCT CGGGATTCGA TGCAGTATGT GCTCTATGTC 
AGACTGGCCA TCCTGGTGAT CGAGTTTGTG TACGCTATAG TGGGCATTGT TTGGCTAACC 
CAGTACTACA CCTCGTGCAA TGACCTCACT GCCAAGAGTG TCACCTTGGG AATGGTAGTG 
TGCAACTGGG TCGTCATTCT GAGTGTCTGC ATCACTGTCC TGTGCGTCTT TGACCCGACA 
GGGCGAACCT TCGTCAAGCT TCGGGCCACA AAGAGAAGGC AGCGCAACCT GAGGACATAC 
AACCTGCGGC ACCGCCTGGA AGAGGGACAG GCCAGCAGCT GGACACGCCG CCTCAAAGTT 
TTTCTATGCT GCACGAGAAC AAAGGATTCC CAGTCAGATG CCTACTCTGA AATTGCCTAC 
CTGTTTGCTG AGTTTTTCCG AGACCTTGAC ATAGTCCCAT CTGACATCAT TGCAGGCCTG 
GTCCTCCTGC GGCAGCGGCA GAGGGCAAAA CGCAGCGCGG TGCTAGATGA GGCGAACAAT 
GACATTCTGG CCTTTCTGTC TGGGATGCCA GTGACAAGGA ACACCAAGTA CCTGGATCTG 
AAAAATGCAC AAGAGATGCA GCGGTACAAA GAAGTGTGTT ACTACATGCT CTTTGCCCTG 
GCTGCCTATG GCTGGCCCAT GTACCTGATG AGGAAACCCA CCTGTGGACT TTGTCGCCTG 
GCTAGATCCT GCTCATGTTG CTGTCTCTGT CCCTCACGGC CTCGCTATGC ACCTGGTGTC 
ACCATTGAGG AGGATAACTG CTGTGGGTGC AATGCTATTG CCATCCGTCG CCACTTTCTG 
GATGAAAACA TGACCTCTGT GGATATTGTC TACACCTCTT GTCATGATGC TGTCTACGAA 
ACGCCCTTCT ATGTGGCTGT GGACCATGAT AAGAAGAAAG TGGTCATCAG TATCCGAGGA 
ACTCTCTCTC CAAAGGATGC CCTGACTGAC TTAACTGGGG ATGCAGAACG CCTTCCAGTT 
GAGGGTCATC ATGGAACCTG GCTAGGACAC AAGGGAATGG TGTTGTCTGC TGAATACATC 
AAGAAGAAAC TGGAGCAGGA GATGGTTCTT TCTCAAGCCT TTGGGCATGA TTTAGGCAGA 
GGAACAAAGC ACTACGGCCT GATTGTGGTG GGACATTCCC TAGGGGCTGG CACAGCTGCC 
ATTCTGTCCT TCCTTCTGCG TCCCCAGTAC CCATCACTGA AGTGCTTTGC GTATTCTCCC 
CCAGGTGGGC TGCTGAGTGA GGATGCCATG GAGTACTCGA AAGAGTTTGT GACTGCAGTG 
GTGCTTGGCA AAGATCTAGT CCCCAGGATT GGTCTCTCTC AGCTGGAAGG ATTTCGCCGG 
CAGCTTCTTG ATGTTCTCCA GAGAAGTACC AAGCCTAAGT GGCGAATAAT TGTGGGTGCC 
ACAAAGTGTA TACCTAAGTC AGAGCTCCCT GAAGAGATAG AAGGGAATTC CGTGACCAGT 
AACCGTCTCT GGACCCACCC CAGCGACCTA ACCATTGCCC TGTCTGCCAG TACACCACTC 
TACCCACCCG GCCGCATCAT CCATGTGGTG CACAATCATC CTGTTGAGCA GTGCTGTTGC 
TGTGACCAGG AGGATCCCAC ATATTTTGCC ATTTGGGGGA ACAATAAAGC ATTTAATGAA 
GTGATCATCT CTCCAGCAAT GCTGCATGAG CACCTTCCCT ATGTGGTCAT GGAGGGACTC 
AACAAGGTCC TGGAGAACTA CAACAAGGGC AAGACTGCCT TACTTTCTGC AGCCAAAGTA 
ATGGTGAGTC CCACAGAAGT GGATCTCAAC CCAGAGTTGA TCTTCCAGAA TCAGCCCTTG 
CCCAGCGGGC CTGCAGTACA GGTTGGAACT GGAGCCATAT TAGCAGACAG AAGGAACAGC 
AGCATCAAGA GCAAATCACA ATCTGAAATC AGCTTGGAGG GGTTTTATGA GACCAAGCCC 
CTTTCTTCTG TGCAGAAGGA CCCTGTAGAG CTTCTGCTCC TGGATACCAA GGAGCGCCTG 
TCTGTGGAGC TGCAGGACCG CCGTGCTCCT CTGGCCACCA TGGAGAGTCT GTCCGATAAT 
GAGTCCATCT ACAGCTTTGA TTCACGGCGC TCCTCAGGCT TCCGGAGCAT CCGTGGCTCC 
CCCAGTCTCC ATGCTGTCAT GGAGAAGGAT GAGACGCACT GTTTTTATAT AGACCCCGTT 
ATCCCTGAGG AGAACCCCTC CCTCAGCTCG AGGACAGAGC TGCTTGCTGC TGACAGCCTC 
TCTAAACATT CACAGGAGAC ACAACCCCCT GAGACCTTGC ATAACAGTGG TGGCACTACC 
CCACAGCGAT GCTGCAGCGA GGAAGGGGCT GCCAGCGAAG GAGACAGAAT TTCCTTTACA 
GGCCGAGAGG AGCTGTCCCT CCAGAATGGA CGGCTCCCTG ATGTGCCCAG TCCACAAGTG 
CTGGAGTTTG CTGAGTTTAT AGACAGCCTC TTCAATCTGG ACAGCAAAAG CAGCTCCTTC 
CAGGACATCT ATTGCATGAT GGTATCAGAT AGCTCCAGTG ACTTTGCAGA GATGCCTAAG 
TCTGTCAGTG ATCAGGAGAT CCTGCTGAGA GCACAGTATG AGCCCAACCT GGTCCCCAAG 
CCCCCTAGGT TGTTCGCAGG CTCAACTGAT CCCTCTTCAG GCATCTCAGT CTCACCCTCC 
TTCCCTCTGA GTTCATCTGG AGAACTTATG GACATTACTC CCACAGGCAT GAGCAGCCAG 
GAATGCCTGG CCACTGACAA AATCCGGACT TCCACCCCGT CTGGTCACAT GACCAGCCCC 
ACCAAGCAGG AGGACCTCAT GATCTCAGCC CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAGLA ( XM_014521047.1 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_014376533.1 Alligator sinensis diacylglycerol lipase, alpha (DAGLA), mRNA.

Price & Availability↑

CloneID	OAl20441
Clone ID Related Accession (Same CDS sequence)	XM_025206945.1 , XM_014521047.1
Accession Version	XM_014521047.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-29
Organism	Alligator sinensis(Chinese alligator)
Product	sn1-specific diacylglycerol lipase alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005842176.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGGA TTGTGGTGTT CCGCCGTCGC TGGTCTGTAG GCAGCGATGA CCTCGTTTTG 
CCAGCCATCT TCCTTTTCCT CCTTCATACC ACCTGGTTTG TGATCCTTTC TGTGGTGCTC 
TTTGGGCTGG TGTACAATCC CAACGAGACC TGTTCCCTTA ACCTGGTGGA CCACGGCAGA 
GGCTACCTGG GCATCCTTCT CAGCTGCATG ATAGCAGAAG TGGCAATCAT CTGGCTCAGC 
ATGAGAGGCA GCATCCTGTA CACAGAGCCT CGGGATTCGA TGCAGTATGT GCTCTATGTC 
AGACTGGCCA TCCTGGTGAT CGAGTTTGTG TACGCTATAG TGGGCATTGT TTGGCTAACC 
CAGTACTACA CCTCGTGCAA TGACCTCACT GCCAAGAGTG TCACCTTGGG AATGGTAGTG 
TGCAACTGGG TCGTCATTCT GAGTGTCTGC ATCACTGTCC TGTGCGTCTT TGACCCGACA 
GGGCGAACCT TCGTCAAGCT TCGGGCCACA AAGAGAAGGC AGCGCAACCT GAGGACATAC 
AACCTGCGGC ACCGCCTGGA AGAGGGACAG GCCAGCAGCT GGACACGCCG CCTCAAAGTT 
TTTCTATGCT GCACGAGAAC AAAGGATTCC CAGTCAGATG CCTACTCTGA AATTGCCTAC 
CTGTTTGCTG AGTTTTTCCG AGACCTTGAC ATAGTCCCAT CTGACATCAT TGCAGGCCTG 
GTCCTCCTGC GGCAGCGGCA GAGGGCAAAA CGCAGCGCGG TGCTAGATGA GGCGAACAAT 
GACATTCTGG CCTTTCTGTC TGGGATGCCA GTGACAAGGA ACACCAAGTA CCTGGATCTG 
AAAAATGCAC AAGAGATGCA GCGGTACAAA GAAGTGTGTT ACTACATGCT CTTTGCCCTG 
GCTGCCTATG GCTGGCCCAT GTACCTGATG AGGAAACCCA CCTGTGGACT TTGTCGCCTG 
GCTAGATCCT GCTCATGTTG CTGTCTCTGT CCCTCACGGC CTCGCTATGC ACCTGGTGTC 
ACCATTGAGG AGGATAACTG CTGTGGGTGC AATGCTATTG CCATCCGTCG CCACTTTCTG 
GATGAAAACA TGACCTCTGT GGATATTGTC TACACCTCTT GTCATGATGC TGTCTACGAA 
ACGCCCTTCT ATGTGGCTGT GGACCATGAT AAGAAGAAAG TGGTCATCAG TATCCGAGGA 
ACTCTCTCTC CAAAGGATGC CCTGACTGAC TTAACTGGGG ATGCAGAACG CCTTCCAGTT 
GAGGGTCATC ATGGAACCTG GCTAGGACAC AAGGGAATGG TGTTGTCTGC TGAATACATC 
AAGAAGAAAC TGGAGCAGGA GATGGTTCTT TCTCAAGCCT TTGGGCATGA TTTAGGCAGA 
GGAACAAAGC ACTACGGCCT GATTGTGGTG GGACATTCCC TAGGGGCTGG CACAGCTGCC 
ATTCTGTCCT TCCTTCTGCG TCCCCAGTAC CCATCACTGA AGTGCTTTGC GTATTCTCCC 
CCAGGTGGGC TGCTGAGTGA GGATGCCATG GAGTACTCGA AAGAGTTTGT GACTGCAGTG 
GTGCTTGGCA AAGATCTAGT CCCCAGGATT GGTCTCTCTC AGCTGGAAGG ATTTCGCCGG 
CAGCTTCTTG ATGTTCTCCA GAGAAGTACC AAGCCTAAGT GGCGAATAAT TGTGGGTGCC 
ACAAAGTGTA TACCTAAGTC AGAGCTCCCT GAAGAGATAG AAGGGAATTC CGTGACCAGT 
AACCGTCTCT GGACCCACCC CAGCGACCTA ACCATTGCCC TGTCTGCCAG TACACCACTC 
TACCCACCCG GCCGCATCAT CCATGTGGTG CACAATCATC CTGTTGAGCA GTGCTGTTGC 
TGTGACCAGG AGGATCCCAC ATATTTTGCC ATTTGGGGGA ACAATAAAGC ATTTAATGAA 
GTGATCATCT CTCCAGCAAT GCTGCATGAG CACCTTCCCT ATGTGGTCAT GGAGGGACTC 
AACAAGGTCC TGGAGAACTA CAACAAGGGC AAGACTGCCT TACTTTCTGC AGCCAAAGTA 
ATGGTGAGTC CCACAGAAGT GGATCTCAAC CCAGAGTTGA TCTTCCAGAA TCAGCCCTTG 
CCCAGCGGGC CTGCAGTACA GGTTGGAACT GGAGCCATAT TAGCAGACAG AAGGAACAGC 
AGCATCAAGA GCAAATCACA ATCTGAAATC AGCTTGGAGG GGTTTTATGA GACCAAGCCC 
CTTTCTTCTG TGCAGAAGGA CCCTGTAGAG CTTCTGCTCC TGGATACCAA GGAGCGCCTG 
TCTGTGGAGC TGCAGGACCG CCGTGCTCCT CTGGCCACCA TGGAGAGTCT GTCCGATAAT 
GAGTCCATCT ACAGCTTTGA TTCACGGCGC TCCTCAGGCT TCCGGAGCAT CCGTGGCTCC 
CCCAGTCTCC ATGCTGTCAT GGAGAAGGAT GAGACGCACT GTTTTTATAT AGACCCCGTT 
ATCCCTGAGG AGAACCCCTC CCTCAGCTCG AGGACAGAGC TGCTTGCTGC TGACAGCCTC 
TCTAAACATT CACAGGAGAC ACAACCCCCT GAGACCTTGC ATAACAGTGG TGGCACTACC 
CCACAGCGAT GCTGCAGCGA GGAAGGGGCT GCCAGCGAAG GAGACAGAAT TTCCTTTACA 
GGCCGAGAGG AGCTGTCCCT CCAGAATGGA CGGCTCCCTG ATGTGCCCAG TCCACAAGTG 
CTGGAGTTTG CTGAGTTTAT AGACAGCCTC TTCAATCTGG ACAGCAAAAG CAGCTCCTTC 
CAGGACATCT ATTGCATGAT GGTATCAGAT AGCTCCAGTG ACTTTGCAGA GATGCCTAAG 
TCTGTCAGTG ATCAGGAGAT CCTGCTGAGA GCACAGTATG AGCCCAACCT GGTCCCCAAG 
CCCCCTAGGT TGTTCGCAGG CTCAACTGAT CCCTCTTCAG GCATCTCAGT CTCACCCTCC 
TTCCCTCTGA GTTCATCTGG AGAACTTATG GACATTACTC CCACAGGCAT GAGCAGCCAG 
GAATGCCTGG CCACTGACAA AATCCGGACT TCCACCCCGT CTGGTCACAT GACCAGCCCC 
ACCAAGCAGG AGGACCTCAT GATCTCAGCC CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014376533.1
CDS	112..3207
Translation	MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPNETCSLNLVDHGRGYLGILLSCMIAEVAIIWLSMRGSILYTEPRDSMQYVLYVRLAILVIEFVYAIVGIVWLTQYYTSCNDLTAKSVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQASSWTRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRSAVLDEANNDILAFLSGMPVTRNTKYLDLKNAQEMQRYKEVCYYMLFALAAYGWPMYLMRKPTCGLCRLARSCSCCCLCPSRPRYAPGVTIEEDNCCGCNAIAIRRHFLDENMTSVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGHDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPSLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGATKCIPKSELPEEIEGNSVTSNRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPVEQCCCCDQEDPTYFAIWGNNKAFNEVIISPAMLHEHLPYVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLNPELIFQNQPLPSGPAVQVGTGAILADRRNSSIKSKSQSEISLEGFYETKPLSSVQKDPVELLLLDTKERLSVELQDRRAPLATMESLSDNESIYSFDSRRSSGFRSIRGSPSLHAVMEKDETHCFYIDPVIPEENPSLSSRTELLAADSLSKHSQETQPPETLHNSGGTTPQRCCSEEGAASEGDRISFTGREELSLQNGRLPDVPSPQVLEFAEFIDSLFNLDSKSSSFQDIYCMMVSDSSSDFAEMPKSVSDQEILLRAQYEPNLVPKPPRLFAGSTDPSSGISVSPSFPLSSSGELMDITPTGMSSQECLATDKIRTSTPSGHMTSPTKQEDLMISAL

Target ORF information:

RefSeq Version	XM_014521047.1
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis diacylglycerol lipase, alpha (DAGLA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014521047.1

ORF Insert Sequence:

ATGCCAGGGA TTGTGGTGTT CCGCCGTCGC TGGTCTGTAG GCAGCGATGA CCTCGTTTTG 
CCAGCCATCT TCCTTTTCCT CCTTCATACC ACCTGGTTTG TGATCCTTTC TGTGGTGCTC 
TTTGGGCTGG TGTACAATCC CAACGAGACC TGTTCCCTTA ACCTGGTGGA CCACGGCAGA 
GGCTACCTGG GCATCCTTCT CAGCTGCATG ATAGCAGAAG TGGCAATCAT CTGGCTCAGC 
ATGAGAGGCA GCATCCTGTA CACAGAGCCT CGGGATTCGA TGCAGTATGT GCTCTATGTC 
AGACTGGCCA TCCTGGTGAT CGAGTTTGTG TACGCTATAG TGGGCATTGT TTGGCTAACC 
CAGTACTACA CCTCGTGCAA TGACCTCACT GCCAAGAGTG TCACCTTGGG AATGGTAGTG 
TGCAACTGGG TCGTCATTCT GAGTGTCTGC ATCACTGTCC TGTGCGTCTT TGACCCGACA 
GGGCGAACCT TCGTCAAGCT TCGGGCCACA AAGAGAAGGC AGCGCAACCT GAGGACATAC 
AACCTGCGGC ACCGCCTGGA AGAGGGACAG GCCAGCAGCT GGACACGCCG CCTCAAAGTT 
TTTCTATGCT GCACGAGAAC AAAGGATTCC CAGTCAGATG CCTACTCTGA AATTGCCTAC 
CTGTTTGCTG AGTTTTTCCG AGACCTTGAC ATAGTCCCAT CTGACATCAT TGCAGGCCTG 
GTCCTCCTGC GGCAGCGGCA GAGGGCAAAA CGCAGCGCGG TGCTAGATGA GGCGAACAAT 
GACATTCTGG CCTTTCTGTC TGGGATGCCA GTGACAAGGA ACACCAAGTA CCTGGATCTG 
AAAAATGCAC AAGAGATGCA GCGGTACAAA GAAGTGTGTT ACTACATGCT CTTTGCCCTG 
GCTGCCTATG GCTGGCCCAT GTACCTGATG AGGAAACCCA CCTGTGGACT TTGTCGCCTG 
GCTAGATCCT GCTCATGTTG CTGTCTCTGT CCCTCACGGC CTCGCTATGC ACCTGGTGTC 
ACCATTGAGG AGGATAACTG CTGTGGGTGC AATGCTATTG CCATCCGTCG CCACTTTCTG 
GATGAAAACA TGACCTCTGT GGATATTGTC TACACCTCTT GTCATGATGC TGTCTACGAA 
ACGCCCTTCT ATGTGGCTGT GGACCATGAT AAGAAGAAAG TGGTCATCAG TATCCGAGGA 
ACTCTCTCTC CAAAGGATGC CCTGACTGAC TTAACTGGGG ATGCAGAACG CCTTCCAGTT 
GAGGGTCATC ATGGAACCTG GCTAGGACAC AAGGGAATGG TGTTGTCTGC TGAATACATC 
AAGAAGAAAC TGGAGCAGGA GATGGTTCTT TCTCAAGCCT TTGGGCATGA TTTAGGCAGA 
GGAACAAAGC ACTACGGCCT GATTGTGGTG GGACATTCCC TAGGGGCTGG CACAGCTGCC 
ATTCTGTCCT TCCTTCTGCG TCCCCAGTAC CCATCACTGA AGTGCTTTGC GTATTCTCCC 
CCAGGTGGGC TGCTGAGTGA GGATGCCATG GAGTACTCGA AAGAGTTTGT GACTGCAGTG 
GTGCTTGGCA AAGATCTAGT CCCCAGGATT GGTCTCTCTC AGCTGGAAGG ATTTCGCCGG 
CAGCTTCTTG ATGTTCTCCA GAGAAGTACC AAGCCTAAGT GGCGAATAAT TGTGGGTGCC 
ACAAAGTGTA TACCTAAGTC AGAGCTCCCT GAAGAGATAG AAGGGAATTC CGTGACCAGT 
AACCGTCTCT GGACCCACCC CAGCGACCTA ACCATTGCCC TGTCTGCCAG TACACCACTC 
TACCCACCCG GCCGCATCAT CCATGTGGTG CACAATCATC CTGTTGAGCA GTGCTGTTGC 
TGTGACCAGG AGGATCCCAC ATATTTTGCC ATTTGGGGGA ACAATAAAGC ATTTAATGAA 
GTGATCATCT CTCCAGCAAT GCTGCATGAG CACCTTCCCT ATGTGGTCAT GGAGGGACTC 
AACAAGGTCC TGGAGAACTA CAACAAGGGC AAGACTGCCT TACTTTCTGC AGCCAAAGTA 
ATGGTGAGTC CCACAGAAGT GGATCTCAAC CCAGAGTTGA TCTTCCAGAA TCAGCCCTTG 
CCCAGCGGGC CTGCAGTACA GGTTGGAACT GGAGCCATAT TAGCAGACAG AAGGAACAGC 
AGCATCAAGA GCAAATCACA ATCTGAAATC AGCTTGGAGG GGTTTTATGA GACCAAGCCC 
CTTTCTTCTG TGCAGAAGGA CCCTGTAGAG CTTCTGCTCC TGGATACCAA GGAGCGCCTG 
TCTGTGGAGC TGCAGGACCG CCGTGCTCCT CTGGCCACCA TGGAGAGTCT GTCCGATAAT 
GAGTCCATCT ACAGCTTTGA TTCACGGCGC TCCTCAGGCT TCCGGAGCAT CCGTGGCTCC 
CCCAGTCTCC ATGCTGTCAT GGAGAAGGAT GAGACGCACT GTTTTTATAT AGACCCCGTT 
ATCCCTGAGG AGAACCCCTC CCTCAGCTCG AGGACAGAGC TGCTTGCTGC TGACAGCCTC 
TCTAAACATT CACAGGAGAC ACAACCCCCT GAGACCTTGC ATAACAGTGG TGGCACTACC 
CCACAGCGAT GCTGCAGCGA GGAAGGGGCT GCCAGCGAAG GAGACAGAAT TTCCTTTACA 
GGCCGAGAGG AGCTGTCCCT CCAGAATGGA CGGCTCCCTG ATGTGCCCAG TCCACAAGTG 
CTGGAGTTTG CTGAGTTTAT AGACAGCCTC TTCAATCTGG ACAGCAAAAG CAGCTCCTTC 
CAGGACATCT ATTGCATGAT GGTATCAGAT AGCTCCAGTG ACTTTGCAGA GATGCCTAAG 
TCTGTCAGTG ATCAGGAGAT CCTGCTGAGA GCACAGTATG AGCCCAACCT GGTCCCCAAG 
CCCCCTAGGT TGTTCGCAGG CTCAACTGAT CCCTCTTCAG GCATCTCAGT CTCACCCTCC 
TTCCCTCTGA GTTCATCTGG AGAACTTATG GACATTACTC CCACAGGCAT GAGCAGCCAG 
GAATGCCTGG CCACTGACAA AATCCGGACT TCCACCCCGT CTGGTCACAT GACCAGCCCC 
ACCAAGCAGG AGGACCTCAT GATCTCAGCC CTCTAG