HECW2 cDNA ORF clone, Alligator mississippiensis(American alligator)

Gene
Clones
gRNAs

Summary

Gene Symbol	HECW2
Entrez Gene ID	106736862
Full Name	HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2
Gene Type	protein-coding
Organism	Alligator mississippiensis(American alligator)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019492111.1	XP_019347656.1	E3 ubiquitin-protein ligase HECW2 isoform X1
XM_019492110.1	XP_019347655.1	E3 ubiquitin-protein ligase HECW2 isoform X1
XM_019492113.1	XP_019347658.1	E3 ubiquitin-protein ligase HECW2 isoform X2
XM_019492109.1	XP_019347654.1	E3 ubiquitin-protein ligase HECW2 isoform X1
XM_019492112.1	XP_019347657.1	E3 ubiquitin-protein ligase HECW2 isoform X1

Pathways from BioSystems
Gene Ontology

The following HECW2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECW2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAl62121	XM_019492113.1 Latest version!	Alligator mississippiensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OAl62120	XM_019492111.1 Latest version!	Alligator mississippiensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAl62120	XM_019492109.1 Latest version!	Alligator mississippiensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAl62120	XM_019492112.1 Latest version!	Alligator mississippiensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAl62120	XM_019492110.1 Latest version!	Alligator mississippiensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HECW2 ( XM_019492113.1 ) cDNA ORF clone, Alligator mississippiensis(American alligator) -> XP_019347658.1 Alligator mississippiensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OAl62121
Clone ID Related Accession (Same CDS sequence)	XM_019492113.1
Accession Version	XM_019492113.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-08
Organism	Alligator mississippiensis(American alligator)
Product	E3 ubiquitin-protein ligase HECW2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017711096.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator mississippiensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGCCG GACATCATGA CACACATCCA GTTTGTTCAA ATGGTTCAGT TCTTGAGGAA 
TCTTCTGGAG AGGCAATACC AAAGCATCAT TTCAGGACTA GTACGGCTCT AGAAACAGAC 
CAGGAGGAAT TAACTTCTTC TACTTCAAGG TCATCACCTA CCAGAGGTCG CCAGGACTCA 
CTGAATGACT ATCTGGATGC AATAGAACAC AGCAGTCATC CGAGACCTGG AACATCTGGT 
GGCAGTGAGC GTTCACTTGG GGCCTCCCCA AAACTAAGAA GTAGCTTTCC CACTGACACA 
AGACTTAATG CTATGCTCCA CATTGATTCA GATGAAGAGG AGCATGAGTT GCATCAAGAT 
ATGGGTTTTC AGTCTTCCTT GGAAGAAGAA GGTGGTTTAG TGATGCTTAA TGGTGCTACA 
AGGAATGTTG AAGAACATTG TTCAAGTCAC TCATCAAATC AGGTAATGCA GGGTTCAGCA 
GACAATGGAA AAGTTTTATC TTTTGAAACT CCTTTGCAGC CATCTGATGA CCAGCAAGAG 
ACTCTCCAAA GGCTTCCACC ATCAGACGTG GCAATTGAAG GTGAAAACCA GCAAGACTGT 
CAAAATGAAG TGCCAGTAGA CCAGGCTGGC ATTGAATTGT GTGCCTGTCA GGAGGCAGAA 
CAGCCTACTA GCAGTGCTGA TACAGGAGCT ATTGACTCTG CTGTTGGTAA TAGAAGAGGT 
ATTAGTGAAA CAGAATCTAT TGACCAAGGG TCTGAACCTT CTCAGGTGTC ATCAGAAACT 
GAGCACAGTG ACCCTGCTCG CACAGAGAGT GTTAGTGAGG CTAGTACTAG GCCAGAAGGG 
GAGAGTGATG TTGAAGGAGC AGACAGCTCT TGTAATGAAA GTGTGACCAC ACGGCTGTCG 
TCAGTTGAAA CACGATGTTC ATCTTTTGAA AGTGCCAGGT TTCCTGAGAC TCCTGCCTTT 
TCATCTCAGG AAGAGGAGGA GGGTGCTTGT GCCACTCATG TGGCTAGAAG TGAGCCTGCT 
GCAGAAACAC AGGAGACTAT GCGCTGTCCT GGCTCTTTAC CTGTAGTGAG ATTACCAAGC 
ACTCAAGAGG AGGTGCAGCT GGCTGAAGGA GGTAGTTTAC AGGATGGAGA GGAAGCAGAA 
GAGATTTGGC AAAGAAGGAG GAGCCTGCAG GCTGCTGCTG CTGCTGCTGC TGCTGCAAGC 
AGCCAGTCCC AGGATGAAGA AACTGGAGAT GCCCAGGAAG CTTGTGAGGG AGCAGCTGCA 
CCAATTGAAG GAGCTACTGG AGGTTCTCAA GCAAATGGCC ACCAAGCTTT GAGGTCACTA 
CCTTCGGTGC GTCAAGATGT CAGCCGATAT CAAAGAGTGG ATGAGGCTCT ACCACCAAAT 
TGGGAGGCTC GGATCGACAG CCATGGAAGG ATATTCTATG TGGACCATGT AAACAGAACT 
ACAACATGGC AGCGACCCAC AGCTCCCCCT GCCCCACAAA TCCTCCAGAG GTCAAACTCT 
ATTCAGCAAA TGGAGCAGCT GAACCGCCGG TATCAGAGTA TCCGACGAAC AATGACTAAT 
GACAGGCCTG AAGAGCACGC AAACACTGTT GATGGAGCTG GAGAGGAAAC TGACCTCCAC 
CCTTCTAATG CAGATTTTCG AAGGGAGAAT GTGCTGCCTC ACTCTACCTC CAGGTCCAGG 
CTTACTTTGT TGCTACAGTC TCCTCCTGTG AAATTTCTTA TCAGTCCAGA ATTCTTTCCA 
GTGCTGCATT CTAACCCTAG TGCCTACCGC ATGTTTACAA ACAACACGTG TTTAAAGCAC 
ATGATCACCA AAGTCCGGCG GGACACCCAC CATTTTGAGC GCTACCAACA TAATCGTGAT 
CTGGTGGGCT TCCTTAATAT GTTTGCTAAC AAACAGCTGG AGCTGCCAAG AGGCTGGGAA 
ATGAAACATG ACCATCAGGG CAAGGCTTTT TTTGTTGACC ACAATTCTCG CACCACCACA 
TTCATCGATC CCCGGCTTCC CTTGCAGAGT AGCAGGCCCA CTAGTGCATT AGTTCATCGG 
CAGCATTTAA CCAGACAACG CAGCCATAGT GCGGGTGAGG TAGGAGAGGA CTCCCGTCAT 
TCAGGACCAC CAGTTTTACC AAGACCATCA AGCACATTTA ATACAGTCAG TAGAGCGCAG 
TATCAGGACA TTGTTCCAGT GGCCTATAAT GACAAGATTG TTGCATTCCT GCGACAGCCC 
AATATCTTTG AAATTCTACA GGAGCGTCAA CCAGATCTCA CCAGGAATCA TTCACTCAGG 
GAGAAGATTC AGTTTATTCG AACAGAGGGA ACCCCAGGGC TGGTGCGTCT ATCCAGTGAT 
GCAGACCTTG TCATGTTACT GAGTTTGTTT GAAGAAGAGA TAATGTCATA TGTGCCGCCA 
CATGCCTTAC TTCATCCCAG TTATTGCCAG TCCCCAAGAG GCTCCCCAGT GTCCTCACCT 
CAGAACTCTC CAGGTACTCA GCGTGCCAAT GCCCGAGCTC CAGCTCCTTA TAAAAGAGAT 
TTTGAAGCCA AGCTGAGGAA CTTCTACAGG AAGCTAGAGA CTAAAGGATA TGGACAAGGC 
CCAGGGAAAC TAAAGTTAAT CATCAGAAGA GACCACTTGC TGGAAGATGC CTTTAACCAG 
ATTATGGGTT ACTCAAGAAA AGACCTACAG AGAAATAAAC TCTATGTCAC GTTTGTTGGG 
GAGGAAGGGT TGGATTACAG TGGACCTTCA AGAGAATTTT TCTTTCTGGT GTCCAGAGAA 
CTATTCAATC CATATTATGG TTTATTTGAA TATTCAGCCA ATGATACCTA CACAGTTCAA 
ATTAGTCCCA TGTCTGCTTT TGTGGACAAT CACCATGAGT GGTTCAGGTT TAGTGGTCGA 
ATCCTTGGTC TTGCTCTGAT ACATCAATAT CTGCTAGATG CCTTCTTTAC CCGGCCCTTT 
TATAAAGCCC TCCTCCGAAT ACTATGTGAC CTGAGTGACT TGGAATACCT TGATGAGGAG 
TTCCACCAGA GTTTGCAGTG GATGAAAGAC AACGATATAC ATGATATCCT AGATCTCACA 
TTCACTGTAA ATGAAGAAGT TTTTGGGCAG ATCACTGAAC GGGAATTAAA GCCAGGAGGT 
GCCAACATCC CAGTCACAGA GAAAAACAAA AAAGAATACA TAGAGAGAAT GGTGAAATGG 
AGAATTGAGA GAGGGGTTGT GCAGCAAACA GAAAGTCTAG TTCGTGGTTT CTATGAGGTG 
GTTGATGCTC GACTTGTGTC TGTATTTGAT GCAAGAGAAC TGGAACTGGT TATTGCTGGC 
ACAGCAGAAA TTGACTTAAG TGACTGGAGA AATAACACAG AGTATAGAGG AGGTTACCAT 
GACAATCATA TTGTAATTCG CTGGTTCTGG GCTACTGTAG AAAGGTTCAA CAATGAACAA 
AGACTAAGGC TGTTACAGTT TGTTACAGGC ACCTCCAGCA TACCTTATGA AGGCTTTGCC 
TCTCTGAGAG GGAGCAATGG GCCAAGAAGA TTCTGTGTAG AAAAATGGGG GAAAATCACT 
GCTCTTCCAA GGGCTCATAC TTGTTTCAAT CGTCTTGATC TTCCCCCTTA CCCATCCTTC 
TCCATGCTGT ATGAGAAACT GTTGACAGCA GTTGAAGAGA CAAGTACTTT TGGACTTGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019347658.1
CDS	202..3864
Translation	MPAGHHDTHPVCSNGSVLEESSGEAIPKHHFRTSTALETDQEELTSSTSRSSPTRGRQDSLNDYLDAIEHSSHPRPGTSGGSERSLGASPKLRSSFPTDTRLNAMLHIDSDEEEHELHQDMGFQSSLEEEGGLVMLNGATRNVEEHCSSHSSNQVMQGSADNGKVLSFETPLQPSDDQQETLQRLPPSDVAIEGENQQDCQNEVPVDQAGIELCACQEAEQPTSSADTGAIDSAVGNRRGISETESIDQGSEPSQVSSETEHSDPARTESVSEASTRPEGESDVEGADSSCNESVTTRLSSVETRCSSFESARFPETPAFSSQEEEEGACATHVARSEPAAETQETMRCPGSLPVVRLPSTQEEVQLAEGGSLQDGEEAEEIWQRRRSLQAAAAAAAAASSQSQDEETGDAQEACEGAAAPIEGATGGSQANGHQALRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQILQRSNSIQQMEQLNRRYQSIRRTMTNDRPEEHANTVDGAGEETDLHPSNADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFPVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHSGPPVLPRPSSTFNTVSRAQYQDIVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWATVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_019492113.1
Organism	Alligator mississippiensis(American alligator)
Definition	Alligator mississippiensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019492113.1

ORF Insert Sequence:

ATGCCAGCCG GACATCATGA CACACATCCA GTTTGTTCAA ATGGTTCAGT TCTTGAGGAA 
TCTTCTGGAG AGGCAATACC AAAGCATCAT TTCAGGACTA GTACGGCTCT AGAAACAGAC 
CAGGAGGAAT TAACTTCTTC TACTTCAAGG TCATCACCTA CCAGAGGTCG CCAGGACTCA 
CTGAATGACT ATCTGGATGC AATAGAACAC AGCAGTCATC CGAGACCTGG AACATCTGGT 
GGCAGTGAGC GTTCACTTGG GGCCTCCCCA AAACTAAGAA GTAGCTTTCC CACTGACACA 
AGACTTAATG CTATGCTCCA CATTGATTCA GATGAAGAGG AGCATGAGTT GCATCAAGAT 
ATGGGTTTTC AGTCTTCCTT GGAAGAAGAA GGTGGTTTAG TGATGCTTAA TGGTGCTACA 
AGGAATGTTG AAGAACATTG TTCAAGTCAC TCATCAAATC AGGTAATGCA GGGTTCAGCA 
GACAATGGAA AAGTTTTATC TTTTGAAACT CCTTTGCAGC CATCTGATGA CCAGCAAGAG 
ACTCTCCAAA GGCTTCCACC ATCAGACGTG GCAATTGAAG GTGAAAACCA GCAAGACTGT 
CAAAATGAAG TGCCAGTAGA CCAGGCTGGC ATTGAATTGT GTGCCTGTCA GGAGGCAGAA 
CAGCCTACTA GCAGTGCTGA TACAGGAGCT ATTGACTCTG CTGTTGGTAA TAGAAGAGGT 
ATTAGTGAAA CAGAATCTAT TGACCAAGGG TCTGAACCTT CTCAGGTGTC ATCAGAAACT 
GAGCACAGTG ACCCTGCTCG CACAGAGAGT GTTAGTGAGG CTAGTACTAG GCCAGAAGGG 
GAGAGTGATG TTGAAGGAGC AGACAGCTCT TGTAATGAAA GTGTGACCAC ACGGCTGTCG 
TCAGTTGAAA CACGATGTTC ATCTTTTGAA AGTGCCAGGT TTCCTGAGAC TCCTGCCTTT 
TCATCTCAGG AAGAGGAGGA GGGTGCTTGT GCCACTCATG TGGCTAGAAG TGAGCCTGCT 
GCAGAAACAC AGGAGACTAT GCGCTGTCCT GGCTCTTTAC CTGTAGTGAG ATTACCAAGC 
ACTCAAGAGG AGGTGCAGCT GGCTGAAGGA GGTAGTTTAC AGGATGGAGA GGAAGCAGAA 
GAGATTTGGC AAAGAAGGAG GAGCCTGCAG GCTGCTGCTG CTGCTGCTGC TGCTGCAAGC 
AGCCAGTCCC AGGATGAAGA AACTGGAGAT GCCCAGGAAG CTTGTGAGGG AGCAGCTGCA 
CCAATTGAAG GAGCTACTGG AGGTTCTCAA GCAAATGGCC ACCAAGCTTT GAGGTCACTA 
CCTTCGGTGC GTCAAGATGT CAGCCGATAT CAAAGAGTGG ATGAGGCTCT ACCACCAAAT 
TGGGAGGCTC GGATCGACAG CCATGGAAGG ATATTCTATG TGGACCATGT AAACAGAACT 
ACAACATGGC AGCGACCCAC AGCTCCCCCT GCCCCACAAA TCCTCCAGAG GTCAAACTCT 
ATTCAGCAAA TGGAGCAGCT GAACCGCCGG TATCAGAGTA TCCGACGAAC AATGACTAAT 
GACAGGCCTG AAGAGCACGC AAACACTGTT GATGGAGCTG GAGAGGAAAC TGACCTCCAC 
CCTTCTAATG CAGATTTTCG AAGGGAGAAT GTGCTGCCTC ACTCTACCTC CAGGTCCAGG 
CTTACTTTGT TGCTACAGTC TCCTCCTGTG AAATTTCTTA TCAGTCCAGA ATTCTTTCCA 
GTGCTGCATT CTAACCCTAG TGCCTACCGC ATGTTTACAA ACAACACGTG TTTAAAGCAC 
ATGATCACCA AAGTCCGGCG GGACACCCAC CATTTTGAGC GCTACCAACA TAATCGTGAT 
CTGGTGGGCT TCCTTAATAT GTTTGCTAAC AAACAGCTGG AGCTGCCAAG AGGCTGGGAA 
ATGAAACATG ACCATCAGGG CAAGGCTTTT TTTGTTGACC ACAATTCTCG CACCACCACA 
TTCATCGATC CCCGGCTTCC CTTGCAGAGT AGCAGGCCCA CTAGTGCATT AGTTCATCGG 
CAGCATTTAA CCAGACAACG CAGCCATAGT GCGGGTGAGG TAGGAGAGGA CTCCCGTCAT 
TCAGGACCAC CAGTTTTACC AAGACCATCA AGCACATTTA ATACAGTCAG TAGAGCGCAG 
TATCAGGACA TTGTTCCAGT GGCCTATAAT GACAAGATTG TTGCATTCCT GCGACAGCCC 
AATATCTTTG AAATTCTACA GGAGCGTCAA CCAGATCTCA CCAGGAATCA TTCACTCAGG 
GAGAAGATTC AGTTTATTCG AACAGAGGGA ACCCCAGGGC TGGTGCGTCT ATCCAGTGAT 
GCAGACCTTG TCATGTTACT GAGTTTGTTT GAAGAAGAGA TAATGTCATA TGTGCCGCCA 
CATGCCTTAC TTCATCCCAG TTATTGCCAG TCCCCAAGAG GCTCCCCAGT GTCCTCACCT 
CAGAACTCTC CAGGTACTCA GCGTGCCAAT GCCCGAGCTC CAGCTCCTTA TAAAAGAGAT 
TTTGAAGCCA AGCTGAGGAA CTTCTACAGG AAGCTAGAGA CTAAAGGATA TGGACAAGGC 
CCAGGGAAAC TAAAGTTAAT CATCAGAAGA GACCACTTGC TGGAAGATGC CTTTAACCAG 
ATTATGGGTT ACTCAAGAAA AGACCTACAG AGAAATAAAC TCTATGTCAC GTTTGTTGGG 
GAGGAAGGGT TGGATTACAG TGGACCTTCA AGAGAATTTT TCTTTCTGGT GTCCAGAGAA 
CTATTCAATC CATATTATGG TTTATTTGAA TATTCAGCCA ATGATACCTA CACAGTTCAA 
ATTAGTCCCA TGTCTGCTTT TGTGGACAAT CACCATGAGT GGTTCAGGTT TAGTGGTCGA 
ATCCTTGGTC TTGCTCTGAT ACATCAATAT CTGCTAGATG CCTTCTTTAC CCGGCCCTTT 
TATAAAGCCC TCCTCCGAAT ACTATGTGAC CTGAGTGACT TGGAATACCT TGATGAGGAG 
TTCCACCAGA GTTTGCAGTG GATGAAAGAC AACGATATAC ATGATATCCT AGATCTCACA 
TTCACTGTAA ATGAAGAAGT TTTTGGGCAG ATCACTGAAC GGGAATTAAA GCCAGGAGGT 
GCCAACATCC CAGTCACAGA GAAAAACAAA AAAGAATACA TAGAGAGAAT GGTGAAATGG 
AGAATTGAGA GAGGGGTTGT GCAGCAAACA GAAAGTCTAG TTCGTGGTTT CTATGAGGTG 
GTTGATGCTC GACTTGTGTC TGTATTTGAT GCAAGAGAAC TGGAACTGGT TATTGCTGGC 
ACAGCAGAAA TTGACTTAAG TGACTGGAGA AATAACACAG AGTATAGAGG AGGTTACCAT 
GACAATCATA TTGTAATTCG CTGGTTCTGG GCTACTGTAG AAAGGTTCAA CAATGAACAA 
AGACTAAGGC TGTTACAGTT TGTTACAGGC ACCTCCAGCA TACCTTATGA AGGCTTTGCC 
TCTCTGAGAG GGAGCAATGG GCCAAGAAGA TTCTGTGTAG AAAAATGGGG GAAAATCACT 
GCTCTTCCAA GGGCTCATAC TTGTTTCAAT CGTCTTGATC TTCCCCCTTA CCCATCCTTC 
TCCATGCTGT ATGAGAAACT GTTGACAGCA GTTGAAGAGA CAAGTACTTT TGGACTTGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_019492111.1 ) cDNA ORF clone, Alligator mississippiensis(American alligator) -> XP_019347656.1 Alligator mississippiensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OAl62120
Clone ID Related Accession (Same CDS sequence)	XM_019492111.1 , XM_019492110.1 , XM_019492109.1 , XM_019492112.1
Accession Version	XM_019492111.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-08
Organism	Alligator mississippiensis(American alligator)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017711096.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator mississippiensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGTT CGGCCCGGGA CCACCTACTT TTTGTGCGAC GTCGCAACCC CCAACTGCGG 
TATACTTTGA GCCCAGAGAA CCTGCAGAGT CTGGCATCTC AGGGCTCCAT GGCTGAGAAT 
ATGAATTTAC AGCGTGCCAA CAGTGACACT GATCTAGTGA CCTCTGATAG CCGCTCCAGT 
CTGACAGCTA GTATGTATGA GTATACCCTT GGGCACTCTC AGAATCTCAT TATCTTCTGG 
GATATTAAAG AAGAAGTGGA CCCAACAGAT TGGATAGGAC TTTATCATAT AGATGAGAAC 
TCTCCAGCTA ATTTCTGGGA TTCCAAGAAC CGGGGAGTGA CTGGCACACA AAAAGGGCAA 
ATTGTATGGC GAATTGAACC TGGCCCTTAT TTTATGGAAC CTGAAATAAA GATTTGTTTT 
AAATACTACC ATGGCATTAG TGGAGCCCTC CGAGCTACTA CCCCATGTAT CACTGTGAAA 
AATCCTGCTG TGATGATGGG GATGGAAGGC ACAGAGGAAG GTGCTTCTGG TCAGCATTCT 
CGGAAATTAG TCAGCTTTAC ACTTTCAGAT ATTAGGGCAG TTGGGCTAAA AAAGGGGATG 
TTCTTCAACC CTGATCCTTA CCTAAAGATG TCGATTCAAC CAGGGAAGAA AAGTACATTC 
CCTACGTTTG CCCATCATGG TCAGGAAAGA CGGTCTACTA TCATCAGTAA CACAACTAAT 
CCTGTTTGGC ACAGAGAGAA ATATTCTTTT GTTGCACTTC TAACAGATGT CTTGGAGATT 
GAAATTAAAG ACAAATTTGC CAAGAGTCGT CCTATCATCA AGCGCTTTCT TGGGAAGTTA 
ACCATCCCAG TCCAGAGGCT ATTGGAGAGA CATGCAATAG GAGACCAAAT TCTCAGCTAC 
AATCTTGGCA GAAGGCTCCC AGCTGACCAT GTAAGTGGGT ATCTGCAGTT CAAAGTGGAG 
ATTACATCTT CTATTCATGA AGATGCTTCA CCAGAAGCAG TTGGTACTTT GCTAGGAGTG 
AATTCCGTAA ATGGAGATTT GGGAAGCCCC TCTGATGATG AGGACATGCC AGCCGGACAT 
CATGACACAC ATCCAGTTTG TTCAAATGGT TCAGTTCTTG AGGAATCTTC TGGAGAGGCA 
ATACCAAAGC ATCATTTCAG GACTAGTACG GCTCTAGAAA CAGACCAGGA GGAATTAACT 
TCTTCTACTT CAAGGTCATC ACCTACCAGA GGTCGCCAGG ACTCACTGAA TGACTATCTG 
GATGCAATAG AACACAGCAG TCATCCGAGA CCTGGAACAT CTGGTGGCAG TGAGCGTTCA 
CTTGGGGCCT CCCCAAAACT AAGAAGTAGC TTTCCCACTG ACACAAGACT TAATGCTATG 
CTCCACATTG ATTCAGATGA AGAGGAGCAT GAGTTGCATC AAGATATGGG TTTTCAGTCT 
TCCTTGGAAG AAGAAGGTGG TTTAGTGATG CTTAATGGTG CTACAAGGAA TGTTGAAGAA 
CATTGTTCAA GTCACTCATC AAATCAGGTA ATGCAGGGTT CAGCAGACAA TGGAAAAGTT 
TTATCTTTTG AAACTCCTTT GCAGCCATCT GATGACCAGC AAGAGACTCT CCAAAGGCTT 
CCACCATCAG ACGTGGCAAT TGAAGGTGAA AACCAGCAAG ACTGTCAAAA TGAAGTGCCA 
GTAGACCAGG CTGGCATTGA ATTGTGTGCC TGTCAGGAGG CAGAACAGCC TACTAGCAGT 
GCTGATACAG GAGCTATTGA CTCTGCTGTT GGTAATAGAA GAGGTATTAG TGAAACAGAA 
TCTATTGACC AAGGGTCTGA ACCTTCTCAG GTGTCATCAG AAACTGAGCA CAGTGACCCT 
GCTCGCACAG AGAGTGTTAG TGAGGCTAGT ACTAGGCCAG AAGGGGAGAG TGATGTTGAA 
GGAGCAGACA GCTCTTGTAA TGAAAGTGTG ACCACACGGC TGTCGTCAGT TGAAACACGA 
TGTTCATCTT TTGAAAGTGC CAGGTTTCCT GAGACTCCTG CCTTTTCATC TCAGGAAGAG 
GAGGAGGGTG CTTGTGCCAC TCATGTGGCT AGAAGTGAGC CTGCTGCAGA AACACAGGAG 
ACTATGCGCT GTCCTGGCTC TTTACCTGTA GTGAGATTAC CAAGCACTCA AGAGGAGGTG 
CAGCTGGCTG AAGGAGGTAG TTTACAGGAT GGAGAGGAAG CAGAAGAGAT TTGGCAAAGA 
AGGAGGAGCC TGCAGGCTGC TGCTGCTGCT GCTGCTGCTG CAAGCAGCCA GTCCCAGGAT 
GAAGAAACTG GAGATGCCCA GGAAGCTTGT GAGGGAGCAG CTGCACCAAT TGAAGGAGCT 
ACTGGAGGTT CTCAAGCAAA TGGCCACCAA GCTTTGAGGT CACTACCTTC GGTGCGTCAA 
GATGTCAGCC GATATCAAAG AGTGGATGAG GCTCTACCAC CAAATTGGGA GGCTCGGATC 
GACAGCCATG GAAGGATATT CTATGTGGAC CATGTAAACA GAACTACAAC ATGGCAGCGA 
CCCACAGCTC CCCCTGCCCC ACAAATCCTC CAGAGGTCAA ACTCTATTCA GCAAATGGAG 
CAGCTGAACC GCCGGTATCA GAGTATCCGA CGAACAATGA CTAATGACAG GCCTGAAGAG 
CACGCAAACA CTGTTGATGG AGCTGGAGAG GAAACTGACC TCCACCCTTC TAATGCAGAT 
TTTCGAAGGG AGAATGTGCT GCCTCACTCT ACCTCCAGGT CCAGGCTTAC TTTGTTGCTA 
CAGTCTCCTC CTGTGAAATT TCTTATCAGT CCAGAATTCT TTCCAGTGCT GCATTCTAAC 
CCTAGTGCCT ACCGCATGTT TACAAACAAC ACGTGTTTAA AGCACATGAT CACCAAAGTC 
CGGCGGGACA CCCACCATTT TGAGCGCTAC CAACATAATC GTGATCTGGT GGGCTTCCTT 
AATATGTTTG CTAACAAACA GCTGGAGCTG CCAAGAGGCT GGGAAATGAA ACATGACCAT 
CAGGGCAAGG CTTTTTTTGT TGACCACAAT TCTCGCACCA CCACATTCAT CGATCCCCGG 
CTTCCCTTGC AGAGTAGCAG GCCCACTAGT GCATTAGTTC ATCGGCAGCA TTTAACCAGA 
CAACGCAGCC ATAGTGCGGG TGAGGTAGGA GAGGACTCCC GTCATTCAGG ACCACCAGTT 
TTACCAAGAC CATCAAGCAC ATTTAATACA GTCAGTAGAG CGCAGTATCA GGACATTGTT 
CCAGTGGCCT ATAATGACAA GATTGTTGCA TTCCTGCGAC AGCCCAATAT CTTTGAAATT 
CTACAGGAGC GTCAACCAGA TCTCACCAGG AATCATTCAC TCAGGGAGAA GATTCAGTTT 
ATTCGAACAG AGGGAACCCC AGGGCTGGTG CGTCTATCCA GTGATGCAGA CCTTGTCATG 
TTACTGAGTT TGTTTGAAGA AGAGATAATG TCATATGTGC CGCCACATGC CTTACTTCAT 
CCCAGTTATT GCCAGTCCCC AAGAGGCTCC CCAGTGTCCT CACCTCAGAA CTCTCCAGGT 
ACTCAGCGTG CCAATGCCCG AGCTCCAGCT CCTTATAAAA GAGATTTTGA AGCCAAGCTG 
AGGAACTTCT ACAGGAAGCT AGAGACTAAA GGATATGGAC AAGGCCCAGG GAAACTAAAG 
TTAATCATCA GAAGAGACCA CTTGCTGGAA GATGCCTTTA ACCAGATTAT GGGTTACTCA 
AGAAAAGACC TACAGAGAAA TAAACTCTAT GTCACGTTTG TTGGGGAGGA AGGGTTGGAT 
TACAGTGGAC CTTCAAGAGA ATTTTTCTTT CTGGTGTCCA GAGAACTATT CAATCCATAT 
TATGGTTTAT TTGAATATTC AGCCAATGAT ACCTACACAG TTCAAATTAG TCCCATGTCT 
GCTTTTGTGG ACAATCACCA TGAGTGGTTC AGGTTTAGTG GTCGAATCCT TGGTCTTGCT 
CTGATACATC AATATCTGCT AGATGCCTTC TTTACCCGGC CCTTTTATAA AGCCCTCCTC 
CGAATACTAT GTGACCTGAG TGACTTGGAA TACCTTGATG AGGAGTTCCA CCAGAGTTTG 
CAGTGGATGA AAGACAACGA TATACATGAT ATCCTAGATC TCACATTCAC TGTAAATGAA 
GAAGTTTTTG GGCAGATCAC TGAACGGGAA TTAAAGCCAG GAGGTGCCAA CATCCCAGTC 
ACAGAGAAAA ACAAAAAAGA ATACATAGAG AGAATGGTGA AATGGAGAAT TGAGAGAGGG 
GTTGTGCAGC AAACAGAAAG TCTAGTTCGT GGTTTCTATG AGGTGGTTGA TGCTCGACTT 
GTGTCTGTAT TTGATGCAAG AGAACTGGAA CTGGTTATTG CTGGCACAGC AGAAATTGAC 
TTAAGTGACT GGAGAAATAA CACAGAGTAT AGAGGAGGTT ACCATGACAA TCATATTGTA 
ATTCGCTGGT TCTGGGCTAC TGTAGAAAGG TTCAACAATG AACAAAGACT AAGGCTGTTA 
CAGTTTGTTA CAGGCACCTC CAGCATACCT TATGAAGGCT TTGCCTCTCT GAGAGGGAGC 
AATGGGCCAA GAAGATTCTG TGTAGAAAAA TGGGGGAAAA TCACTGCTCT TCCAAGGGCT 
CATACTTGTT TCAATCGTCT TGATCTTCCC CCTTACCCAT CCTTCTCCAT GCTGTATGAG 
AAACTGTTGA CAGCAGTTGA AGAGACAAGT ACTTTTGGAC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019347656.1
CDS	328..5055
Translation	MASSARDHLLFVRRRNPQLRYTLSPENLQSLASQGSMAENMNLQRANSDTDLVTSDSRSSLTASMYEYTLGHSQNLIIFWDIKEEVDPTDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGMEGTEEGASGQHSRKLVSFTLSDIRAVGLKKGMFFNPDPYLKMSIQPGKKSTFPTFAHHGQERRSTIISNTTNPVWHREKYSFVALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERHAIGDQILSYNLGRRLPADHVSGYLQFKVEITSSIHEDASPEAVGTLLGVNSVNGDLGSPSDDEDMPAGHHDTHPVCSNGSVLEESSGEAIPKHHFRTSTALETDQEELTSSTSRSSPTRGRQDSLNDYLDAIEHSSHPRPGTSGGSERSLGASPKLRSSFPTDTRLNAMLHIDSDEEEHELHQDMGFQSSLEEEGGLVMLNGATRNVEEHCSSHSSNQVMQGSADNGKVLSFETPLQPSDDQQETLQRLPPSDVAIEGENQQDCQNEVPVDQAGIELCACQEAEQPTSSADTGAIDSAVGNRRGISETESIDQGSEPSQVSSETEHSDPARTESVSEASTRPEGESDVEGADSSCNESVTTRLSSVETRCSSFESARFPETPAFSSQEEEEGACATHVARSEPAAETQETMRCPGSLPVVRLPSTQEEVQLAEGGSLQDGEEAEEIWQRRRSLQAAAAAAAAASSQSQDEETGDAQEACEGAAAPIEGATGGSQANGHQALRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQILQRSNSIQQMEQLNRRYQSIRRTMTNDRPEEHANTVDGAGEETDLHPSNADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFPVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHSGPPVLPRPSSTFNTVSRAQYQDIVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWATVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_019492111.1
Organism	Alligator mississippiensis(American alligator)
Definition	Alligator mississippiensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019492111.1

ORF Insert Sequence:

ATGGCCAGTT CGGCCCGGGA CCACCTACTT TTTGTGCGAC GTCGCAACCC CCAACTGCGG 
TATACTTTGA GCCCAGAGAA CCTGCAGAGT CTGGCATCTC AGGGCTCCAT GGCTGAGAAT 
ATGAATTTAC AGCGTGCCAA CAGTGACACT GATCTAGTGA CCTCTGATAG CCGCTCCAGT 
CTGACAGCTA GTATGTATGA GTATACCCTT GGGCACTCTC AGAATCTCAT TATCTTCTGG 
GATATTAAAG AAGAAGTGGA CCCAACAGAT TGGATAGGAC TTTATCATAT AGATGAGAAC 
TCTCCAGCTA ATTTCTGGGA TTCCAAGAAC CGGGGAGTGA CTGGCACACA AAAAGGGCAA 
ATTGTATGGC GAATTGAACC TGGCCCTTAT TTTATGGAAC CTGAAATAAA GATTTGTTTT 
AAATACTACC ATGGCATTAG TGGAGCCCTC CGAGCTACTA CCCCATGTAT CACTGTGAAA 
AATCCTGCTG TGATGATGGG GATGGAAGGC ACAGAGGAAG GTGCTTCTGG TCAGCATTCT 
CGGAAATTAG TCAGCTTTAC ACTTTCAGAT ATTAGGGCAG TTGGGCTAAA AAAGGGGATG 
TTCTTCAACC CTGATCCTTA CCTAAAGATG TCGATTCAAC CAGGGAAGAA AAGTACATTC 
CCTACGTTTG CCCATCATGG TCAGGAAAGA CGGTCTACTA TCATCAGTAA CACAACTAAT 
CCTGTTTGGC ACAGAGAGAA ATATTCTTTT GTTGCACTTC TAACAGATGT CTTGGAGATT 
GAAATTAAAG ACAAATTTGC CAAGAGTCGT CCTATCATCA AGCGCTTTCT TGGGAAGTTA 
ACCATCCCAG TCCAGAGGCT ATTGGAGAGA CATGCAATAG GAGACCAAAT TCTCAGCTAC 
AATCTTGGCA GAAGGCTCCC AGCTGACCAT GTAAGTGGGT ATCTGCAGTT CAAAGTGGAG 
ATTACATCTT CTATTCATGA AGATGCTTCA CCAGAAGCAG TTGGTACTTT GCTAGGAGTG 
AATTCCGTAA ATGGAGATTT GGGAAGCCCC TCTGATGATG AGGACATGCC AGCCGGACAT 
CATGACACAC ATCCAGTTTG TTCAAATGGT TCAGTTCTTG AGGAATCTTC TGGAGAGGCA 
ATACCAAAGC ATCATTTCAG GACTAGTACG GCTCTAGAAA CAGACCAGGA GGAATTAACT 
TCTTCTACTT CAAGGTCATC ACCTACCAGA GGTCGCCAGG ACTCACTGAA TGACTATCTG 
GATGCAATAG AACACAGCAG TCATCCGAGA CCTGGAACAT CTGGTGGCAG TGAGCGTTCA 
CTTGGGGCCT CCCCAAAACT AAGAAGTAGC TTTCCCACTG ACACAAGACT TAATGCTATG 
CTCCACATTG ATTCAGATGA AGAGGAGCAT GAGTTGCATC AAGATATGGG TTTTCAGTCT 
TCCTTGGAAG AAGAAGGTGG TTTAGTGATG CTTAATGGTG CTACAAGGAA TGTTGAAGAA 
CATTGTTCAA GTCACTCATC AAATCAGGTA ATGCAGGGTT CAGCAGACAA TGGAAAAGTT 
TTATCTTTTG AAACTCCTTT GCAGCCATCT GATGACCAGC AAGAGACTCT CCAAAGGCTT 
CCACCATCAG ACGTGGCAAT TGAAGGTGAA AACCAGCAAG ACTGTCAAAA TGAAGTGCCA 
GTAGACCAGG CTGGCATTGA ATTGTGTGCC TGTCAGGAGG CAGAACAGCC TACTAGCAGT 
GCTGATACAG GAGCTATTGA CTCTGCTGTT GGTAATAGAA GAGGTATTAG TGAAACAGAA 
TCTATTGACC AAGGGTCTGA ACCTTCTCAG GTGTCATCAG AAACTGAGCA CAGTGACCCT 
GCTCGCACAG AGAGTGTTAG TGAGGCTAGT ACTAGGCCAG AAGGGGAGAG TGATGTTGAA 
GGAGCAGACA GCTCTTGTAA TGAAAGTGTG ACCACACGGC TGTCGTCAGT TGAAACACGA 
TGTTCATCTT TTGAAAGTGC CAGGTTTCCT GAGACTCCTG CCTTTTCATC TCAGGAAGAG 
GAGGAGGGTG CTTGTGCCAC TCATGTGGCT AGAAGTGAGC CTGCTGCAGA AACACAGGAG 
ACTATGCGCT GTCCTGGCTC TTTACCTGTA GTGAGATTAC CAAGCACTCA AGAGGAGGTG 
CAGCTGGCTG AAGGAGGTAG TTTACAGGAT GGAGAGGAAG CAGAAGAGAT TTGGCAAAGA 
AGGAGGAGCC TGCAGGCTGC TGCTGCTGCT GCTGCTGCTG CAAGCAGCCA GTCCCAGGAT 
GAAGAAACTG GAGATGCCCA GGAAGCTTGT GAGGGAGCAG CTGCACCAAT TGAAGGAGCT 
ACTGGAGGTT CTCAAGCAAA TGGCCACCAA GCTTTGAGGT CACTACCTTC GGTGCGTCAA 
GATGTCAGCC GATATCAAAG AGTGGATGAG GCTCTACCAC CAAATTGGGA GGCTCGGATC 
GACAGCCATG GAAGGATATT CTATGTGGAC CATGTAAACA GAACTACAAC ATGGCAGCGA 
CCCACAGCTC CCCCTGCCCC ACAAATCCTC CAGAGGTCAA ACTCTATTCA GCAAATGGAG 
CAGCTGAACC GCCGGTATCA GAGTATCCGA CGAACAATGA CTAATGACAG GCCTGAAGAG 
CACGCAAACA CTGTTGATGG AGCTGGAGAG GAAACTGACC TCCACCCTTC TAATGCAGAT 
TTTCGAAGGG AGAATGTGCT GCCTCACTCT ACCTCCAGGT CCAGGCTTAC TTTGTTGCTA 
CAGTCTCCTC CTGTGAAATT TCTTATCAGT CCAGAATTCT TTCCAGTGCT GCATTCTAAC 
CCTAGTGCCT ACCGCATGTT TACAAACAAC ACGTGTTTAA AGCACATGAT CACCAAAGTC 
CGGCGGGACA CCCACCATTT TGAGCGCTAC CAACATAATC GTGATCTGGT GGGCTTCCTT 
AATATGTTTG CTAACAAACA GCTGGAGCTG CCAAGAGGCT GGGAAATGAA ACATGACCAT 
CAGGGCAAGG CTTTTTTTGT TGACCACAAT TCTCGCACCA CCACATTCAT CGATCCCCGG 
CTTCCCTTGC AGAGTAGCAG GCCCACTAGT GCATTAGTTC ATCGGCAGCA TTTAACCAGA 
CAACGCAGCC ATAGTGCGGG TGAGGTAGGA GAGGACTCCC GTCATTCAGG ACCACCAGTT 
TTACCAAGAC CATCAAGCAC ATTTAATACA GTCAGTAGAG CGCAGTATCA GGACATTGTT 
CCAGTGGCCT ATAATGACAA GATTGTTGCA TTCCTGCGAC AGCCCAATAT CTTTGAAATT 
CTACAGGAGC GTCAACCAGA TCTCACCAGG AATCATTCAC TCAGGGAGAA GATTCAGTTT 
ATTCGAACAG AGGGAACCCC AGGGCTGGTG CGTCTATCCA GTGATGCAGA CCTTGTCATG 
TTACTGAGTT TGTTTGAAGA AGAGATAATG TCATATGTGC CGCCACATGC CTTACTTCAT 
CCCAGTTATT GCCAGTCCCC AAGAGGCTCC CCAGTGTCCT CACCTCAGAA CTCTCCAGGT 
ACTCAGCGTG CCAATGCCCG AGCTCCAGCT CCTTATAAAA GAGATTTTGA AGCCAAGCTG 
AGGAACTTCT ACAGGAAGCT AGAGACTAAA GGATATGGAC AAGGCCCAGG GAAACTAAAG 
TTAATCATCA GAAGAGACCA CTTGCTGGAA GATGCCTTTA ACCAGATTAT GGGTTACTCA 
AGAAAAGACC TACAGAGAAA TAAACTCTAT GTCACGTTTG TTGGGGAGGA AGGGTTGGAT 
TACAGTGGAC CTTCAAGAGA ATTTTTCTTT CTGGTGTCCA GAGAACTATT CAATCCATAT 
TATGGTTTAT TTGAATATTC AGCCAATGAT ACCTACACAG TTCAAATTAG TCCCATGTCT 
GCTTTTGTGG ACAATCACCA TGAGTGGTTC AGGTTTAGTG GTCGAATCCT TGGTCTTGCT 
CTGATACATC AATATCTGCT AGATGCCTTC TTTACCCGGC CCTTTTATAA AGCCCTCCTC 
CGAATACTAT GTGACCTGAG TGACTTGGAA TACCTTGATG AGGAGTTCCA CCAGAGTTTG 
CAGTGGATGA AAGACAACGA TATACATGAT ATCCTAGATC TCACATTCAC TGTAAATGAA 
GAAGTTTTTG GGCAGATCAC TGAACGGGAA TTAAAGCCAG GAGGTGCCAA CATCCCAGTC 
ACAGAGAAAA ACAAAAAAGA ATACATAGAG AGAATGGTGA AATGGAGAAT TGAGAGAGGG 
GTTGTGCAGC AAACAGAAAG TCTAGTTCGT GGTTTCTATG AGGTGGTTGA TGCTCGACTT 
GTGTCTGTAT TTGATGCAAG AGAACTGGAA CTGGTTATTG CTGGCACAGC AGAAATTGAC 
TTAAGTGACT GGAGAAATAA CACAGAGTAT AGAGGAGGTT ACCATGACAA TCATATTGTA 
ATTCGCTGGT TCTGGGCTAC TGTAGAAAGG TTCAACAATG AACAAAGACT AAGGCTGTTA 
CAGTTTGTTA CAGGCACCTC CAGCATACCT TATGAAGGCT TTGCCTCTCT GAGAGGGAGC 
AATGGGCCAA GAAGATTCTG TGTAGAAAAA TGGGGGAAAA TCACTGCTCT TCCAAGGGCT 
CATACTTGTT TCAATCGTCT TGATCTTCCC CCTTACCCAT CCTTCTCCAT GCTGTATGAG 
AAACTGTTGA CAGCAGTTGA AGAGACAAGT ACTTTTGGAC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_019492110.1 ) cDNA ORF clone, Alligator mississippiensis(American alligator) -> XP_019347655.1 Alligator mississippiensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.

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CloneID	OAl62120
Clone ID Related Accession (Same CDS sequence)	XM_019492111.1 , XM_019492110.1 , XM_019492109.1 , XM_019492112.1
Accession Version	XM_019492110.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-08
Organism	Alligator mississippiensis(American alligator)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017711096.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator mississippiensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGTT CGGCCCGGGA CCACCTACTT TTTGTGCGAC GTCGCAACCC CCAACTGCGG 
TATACTTTGA GCCCAGAGAA CCTGCAGAGT CTGGCATCTC AGGGCTCCAT GGCTGAGAAT 
ATGAATTTAC AGCGTGCCAA CAGTGACACT GATCTAGTGA CCTCTGATAG CCGCTCCAGT 
CTGACAGCTA GTATGTATGA GTATACCCTT GGGCACTCTC AGAATCTCAT TATCTTCTGG 
GATATTAAAG AAGAAGTGGA CCCAACAGAT TGGATAGGAC TTTATCATAT AGATGAGAAC 
TCTCCAGCTA ATTTCTGGGA TTCCAAGAAC CGGGGAGTGA CTGGCACACA AAAAGGGCAA 
ATTGTATGGC GAATTGAACC TGGCCCTTAT TTTATGGAAC CTGAAATAAA GATTTGTTTT 
AAATACTACC ATGGCATTAG TGGAGCCCTC CGAGCTACTA CCCCATGTAT CACTGTGAAA 
AATCCTGCTG TGATGATGGG GATGGAAGGC ACAGAGGAAG GTGCTTCTGG TCAGCATTCT 
CGGAAATTAG TCAGCTTTAC ACTTTCAGAT ATTAGGGCAG TTGGGCTAAA AAAGGGGATG 
TTCTTCAACC CTGATCCTTA CCTAAAGATG TCGATTCAAC CAGGGAAGAA AAGTACATTC 
CCTACGTTTG CCCATCATGG TCAGGAAAGA CGGTCTACTA TCATCAGTAA CACAACTAAT 
CCTGTTTGGC ACAGAGAGAA ATATTCTTTT GTTGCACTTC TAACAGATGT CTTGGAGATT 
GAAATTAAAG ACAAATTTGC CAAGAGTCGT CCTATCATCA AGCGCTTTCT TGGGAAGTTA 
ACCATCCCAG TCCAGAGGCT ATTGGAGAGA CATGCAATAG GAGACCAAAT TCTCAGCTAC 
AATCTTGGCA GAAGGCTCCC AGCTGACCAT GTAAGTGGGT ATCTGCAGTT CAAAGTGGAG 
ATTACATCTT CTATTCATGA AGATGCTTCA CCAGAAGCAG TTGGTACTTT GCTAGGAGTG 
AATTCCGTAA ATGGAGATTT GGGAAGCCCC TCTGATGATG AGGACATGCC AGCCGGACAT 
CATGACACAC ATCCAGTTTG TTCAAATGGT TCAGTTCTTG AGGAATCTTC TGGAGAGGCA 
ATACCAAAGC ATCATTTCAG GACTAGTACG GCTCTAGAAA CAGACCAGGA GGAATTAACT 
TCTTCTACTT CAAGGTCATC ACCTACCAGA GGTCGCCAGG ACTCACTGAA TGACTATCTG 
GATGCAATAG AACACAGCAG TCATCCGAGA CCTGGAACAT CTGGTGGCAG TGAGCGTTCA 
CTTGGGGCCT CCCCAAAACT AAGAAGTAGC TTTCCCACTG ACACAAGACT TAATGCTATG 
CTCCACATTG ATTCAGATGA AGAGGAGCAT GAGTTGCATC AAGATATGGG TTTTCAGTCT 
TCCTTGGAAG AAGAAGGTGG TTTAGTGATG CTTAATGGTG CTACAAGGAA TGTTGAAGAA 
CATTGTTCAA GTCACTCATC AAATCAGGTA ATGCAGGGTT CAGCAGACAA TGGAAAAGTT 
TTATCTTTTG AAACTCCTTT GCAGCCATCT GATGACCAGC AAGAGACTCT CCAAAGGCTT 
CCACCATCAG ACGTGGCAAT TGAAGGTGAA AACCAGCAAG ACTGTCAAAA TGAAGTGCCA 
GTAGACCAGG CTGGCATTGA ATTGTGTGCC TGTCAGGAGG CAGAACAGCC TACTAGCAGT 
GCTGATACAG GAGCTATTGA CTCTGCTGTT GGTAATAGAA GAGGTATTAG TGAAACAGAA 
TCTATTGACC AAGGGTCTGA ACCTTCTCAG GTGTCATCAG AAACTGAGCA CAGTGACCCT 
GCTCGCACAG AGAGTGTTAG TGAGGCTAGT ACTAGGCCAG AAGGGGAGAG TGATGTTGAA 
GGAGCAGACA GCTCTTGTAA TGAAAGTGTG ACCACACGGC TGTCGTCAGT TGAAACACGA 
TGTTCATCTT TTGAAAGTGC CAGGTTTCCT GAGACTCCTG CCTTTTCATC TCAGGAAGAG 
GAGGAGGGTG CTTGTGCCAC TCATGTGGCT AGAAGTGAGC CTGCTGCAGA AACACAGGAG 
ACTATGCGCT GTCCTGGCTC TTTACCTGTA GTGAGATTAC CAAGCACTCA AGAGGAGGTG 
CAGCTGGCTG AAGGAGGTAG TTTACAGGAT GGAGAGGAAG CAGAAGAGAT TTGGCAAAGA 
AGGAGGAGCC TGCAGGCTGC TGCTGCTGCT GCTGCTGCTG CAAGCAGCCA GTCCCAGGAT 
GAAGAAACTG GAGATGCCCA GGAAGCTTGT GAGGGAGCAG CTGCACCAAT TGAAGGAGCT 
ACTGGAGGTT CTCAAGCAAA TGGCCACCAA GCTTTGAGGT CACTACCTTC GGTGCGTCAA 
GATGTCAGCC GATATCAAAG AGTGGATGAG GCTCTACCAC CAAATTGGGA GGCTCGGATC 
GACAGCCATG GAAGGATATT CTATGTGGAC CATGTAAACA GAACTACAAC ATGGCAGCGA 
CCCACAGCTC CCCCTGCCCC ACAAATCCTC CAGAGGTCAA ACTCTATTCA GCAAATGGAG 
CAGCTGAACC GCCGGTATCA GAGTATCCGA CGAACAATGA CTAATGACAG GCCTGAAGAG 
CACGCAAACA CTGTTGATGG AGCTGGAGAG GAAACTGACC TCCACCCTTC TAATGCAGAT 
TTTCGAAGGG AGAATGTGCT GCCTCACTCT ACCTCCAGGT CCAGGCTTAC TTTGTTGCTA 
CAGTCTCCTC CTGTGAAATT TCTTATCAGT CCAGAATTCT TTCCAGTGCT GCATTCTAAC 
CCTAGTGCCT ACCGCATGTT TACAAACAAC ACGTGTTTAA AGCACATGAT CACCAAAGTC 
CGGCGGGACA CCCACCATTT TGAGCGCTAC CAACATAATC GTGATCTGGT GGGCTTCCTT 
AATATGTTTG CTAACAAACA GCTGGAGCTG CCAAGAGGCT GGGAAATGAA ACATGACCAT 
CAGGGCAAGG CTTTTTTTGT TGACCACAAT TCTCGCACCA CCACATTCAT CGATCCCCGG 
CTTCCCTTGC AGAGTAGCAG GCCCACTAGT GCATTAGTTC ATCGGCAGCA TTTAACCAGA 
CAACGCAGCC ATAGTGCGGG TGAGGTAGGA GAGGACTCCC GTCATTCAGG ACCACCAGTT 
TTACCAAGAC CATCAAGCAC ATTTAATACA GTCAGTAGAG CGCAGTATCA GGACATTGTT 
CCAGTGGCCT ATAATGACAA GATTGTTGCA TTCCTGCGAC AGCCCAATAT CTTTGAAATT 
CTACAGGAGC GTCAACCAGA TCTCACCAGG AATCATTCAC TCAGGGAGAA GATTCAGTTT 
ATTCGAACAG AGGGAACCCC AGGGCTGGTG CGTCTATCCA GTGATGCAGA CCTTGTCATG 
TTACTGAGTT TGTTTGAAGA AGAGATAATG TCATATGTGC CGCCACATGC CTTACTTCAT 
CCCAGTTATT GCCAGTCCCC AAGAGGCTCC CCAGTGTCCT CACCTCAGAA CTCTCCAGGT 
ACTCAGCGTG CCAATGCCCG AGCTCCAGCT CCTTATAAAA GAGATTTTGA AGCCAAGCTG 
AGGAACTTCT ACAGGAAGCT AGAGACTAAA GGATATGGAC AAGGCCCAGG GAAACTAAAG 
TTAATCATCA GAAGAGACCA CTTGCTGGAA GATGCCTTTA ACCAGATTAT GGGTTACTCA 
AGAAAAGACC TACAGAGAAA TAAACTCTAT GTCACGTTTG TTGGGGAGGA AGGGTTGGAT 
TACAGTGGAC CTTCAAGAGA ATTTTTCTTT CTGGTGTCCA GAGAACTATT CAATCCATAT 
TATGGTTTAT TTGAATATTC AGCCAATGAT ACCTACACAG TTCAAATTAG TCCCATGTCT 
GCTTTTGTGG ACAATCACCA TGAGTGGTTC AGGTTTAGTG GTCGAATCCT TGGTCTTGCT 
CTGATACATC AATATCTGCT AGATGCCTTC TTTACCCGGC CCTTTTATAA AGCCCTCCTC 
CGAATACTAT GTGACCTGAG TGACTTGGAA TACCTTGATG AGGAGTTCCA CCAGAGTTTG 
CAGTGGATGA AAGACAACGA TATACATGAT ATCCTAGATC TCACATTCAC TGTAAATGAA 
GAAGTTTTTG GGCAGATCAC TGAACGGGAA TTAAAGCCAG GAGGTGCCAA CATCCCAGTC 
ACAGAGAAAA ACAAAAAAGA ATACATAGAG AGAATGGTGA AATGGAGAAT TGAGAGAGGG 
GTTGTGCAGC AAACAGAAAG TCTAGTTCGT GGTTTCTATG AGGTGGTTGA TGCTCGACTT 
GTGTCTGTAT TTGATGCAAG AGAACTGGAA CTGGTTATTG CTGGCACAGC AGAAATTGAC 
TTAAGTGACT GGAGAAATAA CACAGAGTAT AGAGGAGGTT ACCATGACAA TCATATTGTA 
ATTCGCTGGT TCTGGGCTAC TGTAGAAAGG TTCAACAATG AACAAAGACT AAGGCTGTTA 
CAGTTTGTTA CAGGCACCTC CAGCATACCT TATGAAGGCT TTGCCTCTCT GAGAGGGAGC 
AATGGGCCAA GAAGATTCTG TGTAGAAAAA TGGGGGAAAA TCACTGCTCT TCCAAGGGCT 
CATACTTGTT TCAATCGTCT TGATCTTCCC CCTTACCCAT CCTTCTCCAT GCTGTATGAG 
AAACTGTTGA CAGCAGTTGA AGAGACAAGT ACTTTTGGAC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019347655.1
CDS	451..5178
Translation	MASSARDHLLFVRRRNPQLRYTLSPENLQSLASQGSMAENMNLQRANSDTDLVTSDSRSSLTASMYEYTLGHSQNLIIFWDIKEEVDPTDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGMEGTEEGASGQHSRKLVSFTLSDIRAVGLKKGMFFNPDPYLKMSIQPGKKSTFPTFAHHGQERRSTIISNTTNPVWHREKYSFVALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERHAIGDQILSYNLGRRLPADHVSGYLQFKVEITSSIHEDASPEAVGTLLGVNSVNGDLGSPSDDEDMPAGHHDTHPVCSNGSVLEESSGEAIPKHHFRTSTALETDQEELTSSTSRSSPTRGRQDSLNDYLDAIEHSSHPRPGTSGGSERSLGASPKLRSSFPTDTRLNAMLHIDSDEEEHELHQDMGFQSSLEEEGGLVMLNGATRNVEEHCSSHSSNQVMQGSADNGKVLSFETPLQPSDDQQETLQRLPPSDVAIEGENQQDCQNEVPVDQAGIELCACQEAEQPTSSADTGAIDSAVGNRRGISETESIDQGSEPSQVSSETEHSDPARTESVSEASTRPEGESDVEGADSSCNESVTTRLSSVETRCSSFESARFPETPAFSSQEEEEGACATHVARSEPAAETQETMRCPGSLPVVRLPSTQEEVQLAEGGSLQDGEEAEEIWQRRRSLQAAAAAAAAASSQSQDEETGDAQEACEGAAAPIEGATGGSQANGHQALRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQILQRSNSIQQMEQLNRRYQSIRRTMTNDRPEEHANTVDGAGEETDLHPSNADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFPVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHSGPPVLPRPSSTFNTVSRAQYQDIVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWATVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_019492110.1
Organism	Alligator mississippiensis(American alligator)
Definition	Alligator mississippiensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019492110.1

ORF Insert Sequence:

ATGGCCAGTT CGGCCCGGGA CCACCTACTT TTTGTGCGAC GTCGCAACCC CCAACTGCGG 
TATACTTTGA GCCCAGAGAA CCTGCAGAGT CTGGCATCTC AGGGCTCCAT GGCTGAGAAT 
ATGAATTTAC AGCGTGCCAA CAGTGACACT GATCTAGTGA CCTCTGATAG CCGCTCCAGT 
CTGACAGCTA GTATGTATGA GTATACCCTT GGGCACTCTC AGAATCTCAT TATCTTCTGG 
GATATTAAAG AAGAAGTGGA CCCAACAGAT TGGATAGGAC TTTATCATAT AGATGAGAAC 
TCTCCAGCTA ATTTCTGGGA TTCCAAGAAC CGGGGAGTGA CTGGCACACA AAAAGGGCAA 
ATTGTATGGC GAATTGAACC TGGCCCTTAT TTTATGGAAC CTGAAATAAA GATTTGTTTT 
AAATACTACC ATGGCATTAG TGGAGCCCTC CGAGCTACTA CCCCATGTAT CACTGTGAAA 
AATCCTGCTG TGATGATGGG GATGGAAGGC ACAGAGGAAG GTGCTTCTGG TCAGCATTCT 
CGGAAATTAG TCAGCTTTAC ACTTTCAGAT ATTAGGGCAG TTGGGCTAAA AAAGGGGATG 
TTCTTCAACC CTGATCCTTA CCTAAAGATG TCGATTCAAC CAGGGAAGAA AAGTACATTC 
CCTACGTTTG CCCATCATGG TCAGGAAAGA CGGTCTACTA TCATCAGTAA CACAACTAAT 
CCTGTTTGGC ACAGAGAGAA ATATTCTTTT GTTGCACTTC TAACAGATGT CTTGGAGATT 
GAAATTAAAG ACAAATTTGC CAAGAGTCGT CCTATCATCA AGCGCTTTCT TGGGAAGTTA 
ACCATCCCAG TCCAGAGGCT ATTGGAGAGA CATGCAATAG GAGACCAAAT TCTCAGCTAC 
AATCTTGGCA GAAGGCTCCC AGCTGACCAT GTAAGTGGGT ATCTGCAGTT CAAAGTGGAG 
ATTACATCTT CTATTCATGA AGATGCTTCA CCAGAAGCAG TTGGTACTTT GCTAGGAGTG 
AATTCCGTAA ATGGAGATTT GGGAAGCCCC TCTGATGATG AGGACATGCC AGCCGGACAT 
CATGACACAC ATCCAGTTTG TTCAAATGGT TCAGTTCTTG AGGAATCTTC TGGAGAGGCA 
ATACCAAAGC ATCATTTCAG GACTAGTACG GCTCTAGAAA CAGACCAGGA GGAATTAACT 
TCTTCTACTT CAAGGTCATC ACCTACCAGA GGTCGCCAGG ACTCACTGAA TGACTATCTG 
GATGCAATAG AACACAGCAG TCATCCGAGA CCTGGAACAT CTGGTGGCAG TGAGCGTTCA 
CTTGGGGCCT CCCCAAAACT AAGAAGTAGC TTTCCCACTG ACACAAGACT TAATGCTATG 
CTCCACATTG ATTCAGATGA AGAGGAGCAT GAGTTGCATC AAGATATGGG TTTTCAGTCT 
TCCTTGGAAG AAGAAGGTGG TTTAGTGATG CTTAATGGTG CTACAAGGAA TGTTGAAGAA 
CATTGTTCAA GTCACTCATC AAATCAGGTA ATGCAGGGTT CAGCAGACAA TGGAAAAGTT 
TTATCTTTTG AAACTCCTTT GCAGCCATCT GATGACCAGC AAGAGACTCT CCAAAGGCTT 
CCACCATCAG ACGTGGCAAT TGAAGGTGAA AACCAGCAAG ACTGTCAAAA TGAAGTGCCA 
GTAGACCAGG CTGGCATTGA ATTGTGTGCC TGTCAGGAGG CAGAACAGCC TACTAGCAGT 
GCTGATACAG GAGCTATTGA CTCTGCTGTT GGTAATAGAA GAGGTATTAG TGAAACAGAA 
TCTATTGACC AAGGGTCTGA ACCTTCTCAG GTGTCATCAG AAACTGAGCA CAGTGACCCT 
GCTCGCACAG AGAGTGTTAG TGAGGCTAGT ACTAGGCCAG AAGGGGAGAG TGATGTTGAA 
GGAGCAGACA GCTCTTGTAA TGAAAGTGTG ACCACACGGC TGTCGTCAGT TGAAACACGA 
TGTTCATCTT TTGAAAGTGC CAGGTTTCCT GAGACTCCTG CCTTTTCATC TCAGGAAGAG 
GAGGAGGGTG CTTGTGCCAC TCATGTGGCT AGAAGTGAGC CTGCTGCAGA AACACAGGAG 
ACTATGCGCT GTCCTGGCTC TTTACCTGTA GTGAGATTAC CAAGCACTCA AGAGGAGGTG 
CAGCTGGCTG AAGGAGGTAG TTTACAGGAT GGAGAGGAAG CAGAAGAGAT TTGGCAAAGA 
AGGAGGAGCC TGCAGGCTGC TGCTGCTGCT GCTGCTGCTG CAAGCAGCCA GTCCCAGGAT 
GAAGAAACTG GAGATGCCCA GGAAGCTTGT GAGGGAGCAG CTGCACCAAT TGAAGGAGCT 
ACTGGAGGTT CTCAAGCAAA TGGCCACCAA GCTTTGAGGT CACTACCTTC GGTGCGTCAA 
GATGTCAGCC GATATCAAAG AGTGGATGAG GCTCTACCAC CAAATTGGGA GGCTCGGATC 
GACAGCCATG GAAGGATATT CTATGTGGAC CATGTAAACA GAACTACAAC ATGGCAGCGA 
CCCACAGCTC CCCCTGCCCC ACAAATCCTC CAGAGGTCAA ACTCTATTCA GCAAATGGAG 
CAGCTGAACC GCCGGTATCA GAGTATCCGA CGAACAATGA CTAATGACAG GCCTGAAGAG 
CACGCAAACA CTGTTGATGG AGCTGGAGAG GAAACTGACC TCCACCCTTC TAATGCAGAT 
TTTCGAAGGG AGAATGTGCT GCCTCACTCT ACCTCCAGGT CCAGGCTTAC TTTGTTGCTA 
CAGTCTCCTC CTGTGAAATT TCTTATCAGT CCAGAATTCT TTCCAGTGCT GCATTCTAAC 
CCTAGTGCCT ACCGCATGTT TACAAACAAC ACGTGTTTAA AGCACATGAT CACCAAAGTC 
CGGCGGGACA CCCACCATTT TGAGCGCTAC CAACATAATC GTGATCTGGT GGGCTTCCTT 
AATATGTTTG CTAACAAACA GCTGGAGCTG CCAAGAGGCT GGGAAATGAA ACATGACCAT 
CAGGGCAAGG CTTTTTTTGT TGACCACAAT TCTCGCACCA CCACATTCAT CGATCCCCGG 
CTTCCCTTGC AGAGTAGCAG GCCCACTAGT GCATTAGTTC ATCGGCAGCA TTTAACCAGA 
CAACGCAGCC ATAGTGCGGG TGAGGTAGGA GAGGACTCCC GTCATTCAGG ACCACCAGTT 
TTACCAAGAC CATCAAGCAC ATTTAATACA GTCAGTAGAG CGCAGTATCA GGACATTGTT 
CCAGTGGCCT ATAATGACAA GATTGTTGCA TTCCTGCGAC AGCCCAATAT CTTTGAAATT 
CTACAGGAGC GTCAACCAGA TCTCACCAGG AATCATTCAC TCAGGGAGAA GATTCAGTTT 
ATTCGAACAG AGGGAACCCC AGGGCTGGTG CGTCTATCCA GTGATGCAGA CCTTGTCATG 
TTACTGAGTT TGTTTGAAGA AGAGATAATG TCATATGTGC CGCCACATGC CTTACTTCAT 
CCCAGTTATT GCCAGTCCCC AAGAGGCTCC CCAGTGTCCT CACCTCAGAA CTCTCCAGGT 
ACTCAGCGTG CCAATGCCCG AGCTCCAGCT CCTTATAAAA GAGATTTTGA AGCCAAGCTG 
AGGAACTTCT ACAGGAAGCT AGAGACTAAA GGATATGGAC AAGGCCCAGG GAAACTAAAG 
TTAATCATCA GAAGAGACCA CTTGCTGGAA GATGCCTTTA ACCAGATTAT GGGTTACTCA 
AGAAAAGACC TACAGAGAAA TAAACTCTAT GTCACGTTTG TTGGGGAGGA AGGGTTGGAT 
TACAGTGGAC CTTCAAGAGA ATTTTTCTTT CTGGTGTCCA GAGAACTATT CAATCCATAT 
TATGGTTTAT TTGAATATTC AGCCAATGAT ACCTACACAG TTCAAATTAG TCCCATGTCT 
GCTTTTGTGG ACAATCACCA TGAGTGGTTC AGGTTTAGTG GTCGAATCCT TGGTCTTGCT 
CTGATACATC AATATCTGCT AGATGCCTTC TTTACCCGGC CCTTTTATAA AGCCCTCCTC 
CGAATACTAT GTGACCTGAG TGACTTGGAA TACCTTGATG AGGAGTTCCA CCAGAGTTTG 
CAGTGGATGA AAGACAACGA TATACATGAT ATCCTAGATC TCACATTCAC TGTAAATGAA 
GAAGTTTTTG GGCAGATCAC TGAACGGGAA TTAAAGCCAG GAGGTGCCAA CATCCCAGTC 
ACAGAGAAAA ACAAAAAAGA ATACATAGAG AGAATGGTGA AATGGAGAAT TGAGAGAGGG 
GTTGTGCAGC AAACAGAAAG TCTAGTTCGT GGTTTCTATG AGGTGGTTGA TGCTCGACTT 
GTGTCTGTAT TTGATGCAAG AGAACTGGAA CTGGTTATTG CTGGCACAGC AGAAATTGAC 
TTAAGTGACT GGAGAAATAA CACAGAGTAT AGAGGAGGTT ACCATGACAA TCATATTGTA 
ATTCGCTGGT TCTGGGCTAC TGTAGAAAGG TTCAACAATG AACAAAGACT AAGGCTGTTA 
CAGTTTGTTA CAGGCACCTC CAGCATACCT TATGAAGGCT TTGCCTCTCT GAGAGGGAGC 
AATGGGCCAA GAAGATTCTG TGTAGAAAAA TGGGGGAAAA TCACTGCTCT TCCAAGGGCT 
CATACTTGTT TCAATCGTCT TGATCTTCCC CCTTACCCAT CCTTCTCCAT GCTGTATGAG 
AAACTGTTGA CAGCAGTTGA AGAGACAAGT ACTTTTGGAC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_019492109.1 ) cDNA ORF clone, Alligator mississippiensis(American alligator) -> XP_019347654.1 Alligator mississippiensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.

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CloneID	OAl62120
Clone ID Related Accession (Same CDS sequence)	XM_019492111.1 , XM_019492110.1 , XM_019492109.1 , XM_019492112.1
Accession Version	XM_019492109.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-08
Organism	Alligator mississippiensis(American alligator)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017711096.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator mississippiensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGTT CGGCCCGGGA CCACCTACTT TTTGTGCGAC GTCGCAACCC CCAACTGCGG 
TATACTTTGA GCCCAGAGAA CCTGCAGAGT CTGGCATCTC AGGGCTCCAT GGCTGAGAAT 
ATGAATTTAC AGCGTGCCAA CAGTGACACT GATCTAGTGA CCTCTGATAG CCGCTCCAGT 
CTGACAGCTA GTATGTATGA GTATACCCTT GGGCACTCTC AGAATCTCAT TATCTTCTGG 
GATATTAAAG AAGAAGTGGA CCCAACAGAT TGGATAGGAC TTTATCATAT AGATGAGAAC 
TCTCCAGCTA ATTTCTGGGA TTCCAAGAAC CGGGGAGTGA CTGGCACACA AAAAGGGCAA 
ATTGTATGGC GAATTGAACC TGGCCCTTAT TTTATGGAAC CTGAAATAAA GATTTGTTTT 
AAATACTACC ATGGCATTAG TGGAGCCCTC CGAGCTACTA CCCCATGTAT CACTGTGAAA 
AATCCTGCTG TGATGATGGG GATGGAAGGC ACAGAGGAAG GTGCTTCTGG TCAGCATTCT 
CGGAAATTAG TCAGCTTTAC ACTTTCAGAT ATTAGGGCAG TTGGGCTAAA AAAGGGGATG 
TTCTTCAACC CTGATCCTTA CCTAAAGATG TCGATTCAAC CAGGGAAGAA AAGTACATTC 
CCTACGTTTG CCCATCATGG TCAGGAAAGA CGGTCTACTA TCATCAGTAA CACAACTAAT 
CCTGTTTGGC ACAGAGAGAA ATATTCTTTT GTTGCACTTC TAACAGATGT CTTGGAGATT 
GAAATTAAAG ACAAATTTGC CAAGAGTCGT CCTATCATCA AGCGCTTTCT TGGGAAGTTA 
ACCATCCCAG TCCAGAGGCT ATTGGAGAGA CATGCAATAG GAGACCAAAT TCTCAGCTAC 
AATCTTGGCA GAAGGCTCCC AGCTGACCAT GTAAGTGGGT ATCTGCAGTT CAAAGTGGAG 
ATTACATCTT CTATTCATGA AGATGCTTCA CCAGAAGCAG TTGGTACTTT GCTAGGAGTG 
AATTCCGTAA ATGGAGATTT GGGAAGCCCC TCTGATGATG AGGACATGCC AGCCGGACAT 
CATGACACAC ATCCAGTTTG TTCAAATGGT TCAGTTCTTG AGGAATCTTC TGGAGAGGCA 
ATACCAAAGC ATCATTTCAG GACTAGTACG GCTCTAGAAA CAGACCAGGA GGAATTAACT 
TCTTCTACTT CAAGGTCATC ACCTACCAGA GGTCGCCAGG ACTCACTGAA TGACTATCTG 
GATGCAATAG AACACAGCAG TCATCCGAGA CCTGGAACAT CTGGTGGCAG TGAGCGTTCA 
CTTGGGGCCT CCCCAAAACT AAGAAGTAGC TTTCCCACTG ACACAAGACT TAATGCTATG 
CTCCACATTG ATTCAGATGA AGAGGAGCAT GAGTTGCATC AAGATATGGG TTTTCAGTCT 
TCCTTGGAAG AAGAAGGTGG TTTAGTGATG CTTAATGGTG CTACAAGGAA TGTTGAAGAA 
CATTGTTCAA GTCACTCATC AAATCAGGTA ATGCAGGGTT CAGCAGACAA TGGAAAAGTT 
TTATCTTTTG AAACTCCTTT GCAGCCATCT GATGACCAGC AAGAGACTCT CCAAAGGCTT 
CCACCATCAG ACGTGGCAAT TGAAGGTGAA AACCAGCAAG ACTGTCAAAA TGAAGTGCCA 
GTAGACCAGG CTGGCATTGA ATTGTGTGCC TGTCAGGAGG CAGAACAGCC TACTAGCAGT 
GCTGATACAG GAGCTATTGA CTCTGCTGTT GGTAATAGAA GAGGTATTAG TGAAACAGAA 
TCTATTGACC AAGGGTCTGA ACCTTCTCAG GTGTCATCAG AAACTGAGCA CAGTGACCCT 
GCTCGCACAG AGAGTGTTAG TGAGGCTAGT ACTAGGCCAG AAGGGGAGAG TGATGTTGAA 
GGAGCAGACA GCTCTTGTAA TGAAAGTGTG ACCACACGGC TGTCGTCAGT TGAAACACGA 
TGTTCATCTT TTGAAAGTGC CAGGTTTCCT GAGACTCCTG CCTTTTCATC TCAGGAAGAG 
GAGGAGGGTG CTTGTGCCAC TCATGTGGCT AGAAGTGAGC CTGCTGCAGA AACACAGGAG 
ACTATGCGCT GTCCTGGCTC TTTACCTGTA GTGAGATTAC CAAGCACTCA AGAGGAGGTG 
CAGCTGGCTG AAGGAGGTAG TTTACAGGAT GGAGAGGAAG CAGAAGAGAT TTGGCAAAGA 
AGGAGGAGCC TGCAGGCTGC TGCTGCTGCT GCTGCTGCTG CAAGCAGCCA GTCCCAGGAT 
GAAGAAACTG GAGATGCCCA GGAAGCTTGT GAGGGAGCAG CTGCACCAAT TGAAGGAGCT 
ACTGGAGGTT CTCAAGCAAA TGGCCACCAA GCTTTGAGGT CACTACCTTC GGTGCGTCAA 
GATGTCAGCC GATATCAAAG AGTGGATGAG GCTCTACCAC CAAATTGGGA GGCTCGGATC 
GACAGCCATG GAAGGATATT CTATGTGGAC CATGTAAACA GAACTACAAC ATGGCAGCGA 
CCCACAGCTC CCCCTGCCCC ACAAATCCTC CAGAGGTCAA ACTCTATTCA GCAAATGGAG 
CAGCTGAACC GCCGGTATCA GAGTATCCGA CGAACAATGA CTAATGACAG GCCTGAAGAG 
CACGCAAACA CTGTTGATGG AGCTGGAGAG GAAACTGACC TCCACCCTTC TAATGCAGAT 
TTTCGAAGGG AGAATGTGCT GCCTCACTCT ACCTCCAGGT CCAGGCTTAC TTTGTTGCTA 
CAGTCTCCTC CTGTGAAATT TCTTATCAGT CCAGAATTCT TTCCAGTGCT GCATTCTAAC 
CCTAGTGCCT ACCGCATGTT TACAAACAAC ACGTGTTTAA AGCACATGAT CACCAAAGTC 
CGGCGGGACA CCCACCATTT TGAGCGCTAC CAACATAATC GTGATCTGGT GGGCTTCCTT 
AATATGTTTG CTAACAAACA GCTGGAGCTG CCAAGAGGCT GGGAAATGAA ACATGACCAT 
CAGGGCAAGG CTTTTTTTGT TGACCACAAT TCTCGCACCA CCACATTCAT CGATCCCCGG 
CTTCCCTTGC AGAGTAGCAG GCCCACTAGT GCATTAGTTC ATCGGCAGCA TTTAACCAGA 
CAACGCAGCC ATAGTGCGGG TGAGGTAGGA GAGGACTCCC GTCATTCAGG ACCACCAGTT 
TTACCAAGAC CATCAAGCAC ATTTAATACA GTCAGTAGAG CGCAGTATCA GGACATTGTT 
CCAGTGGCCT ATAATGACAA GATTGTTGCA TTCCTGCGAC AGCCCAATAT CTTTGAAATT 
CTACAGGAGC GTCAACCAGA TCTCACCAGG AATCATTCAC TCAGGGAGAA GATTCAGTTT 
ATTCGAACAG AGGGAACCCC AGGGCTGGTG CGTCTATCCA GTGATGCAGA CCTTGTCATG 
TTACTGAGTT TGTTTGAAGA AGAGATAATG TCATATGTGC CGCCACATGC CTTACTTCAT 
CCCAGTTATT GCCAGTCCCC AAGAGGCTCC CCAGTGTCCT CACCTCAGAA CTCTCCAGGT 
ACTCAGCGTG CCAATGCCCG AGCTCCAGCT CCTTATAAAA GAGATTTTGA AGCCAAGCTG 
AGGAACTTCT ACAGGAAGCT AGAGACTAAA GGATATGGAC AAGGCCCAGG GAAACTAAAG 
TTAATCATCA GAAGAGACCA CTTGCTGGAA GATGCCTTTA ACCAGATTAT GGGTTACTCA 
AGAAAAGACC TACAGAGAAA TAAACTCTAT GTCACGTTTG TTGGGGAGGA AGGGTTGGAT 
TACAGTGGAC CTTCAAGAGA ATTTTTCTTT CTGGTGTCCA GAGAACTATT CAATCCATAT 
TATGGTTTAT TTGAATATTC AGCCAATGAT ACCTACACAG TTCAAATTAG TCCCATGTCT 
GCTTTTGTGG ACAATCACCA TGAGTGGTTC AGGTTTAGTG GTCGAATCCT TGGTCTTGCT 
CTGATACATC AATATCTGCT AGATGCCTTC TTTACCCGGC CCTTTTATAA AGCCCTCCTC 
CGAATACTAT GTGACCTGAG TGACTTGGAA TACCTTGATG AGGAGTTCCA CCAGAGTTTG 
CAGTGGATGA AAGACAACGA TATACATGAT ATCCTAGATC TCACATTCAC TGTAAATGAA 
GAAGTTTTTG GGCAGATCAC TGAACGGGAA TTAAAGCCAG GAGGTGCCAA CATCCCAGTC 
ACAGAGAAAA ACAAAAAAGA ATACATAGAG AGAATGGTGA AATGGAGAAT TGAGAGAGGG 
GTTGTGCAGC AAACAGAAAG TCTAGTTCGT GGTTTCTATG AGGTGGTTGA TGCTCGACTT 
GTGTCTGTAT TTGATGCAAG AGAACTGGAA CTGGTTATTG CTGGCACAGC AGAAATTGAC 
TTAAGTGACT GGAGAAATAA CACAGAGTAT AGAGGAGGTT ACCATGACAA TCATATTGTA 
ATTCGCTGGT TCTGGGCTAC TGTAGAAAGG TTCAACAATG AACAAAGACT AAGGCTGTTA 
CAGTTTGTTA CAGGCACCTC CAGCATACCT TATGAAGGCT TTGCCTCTCT GAGAGGGAGC 
AATGGGCCAA GAAGATTCTG TGTAGAAAAA TGGGGGAAAA TCACTGCTCT TCCAAGGGCT 
CATACTTGTT TCAATCGTCT TGATCTTCCC CCTTACCCAT CCTTCTCCAT GCTGTATGAG 
AAACTGTTGA CAGCAGTTGA AGAGACAAGT ACTTTTGGAC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019347654.1
CDS	570..5297
Translation	MASSARDHLLFVRRRNPQLRYTLSPENLQSLASQGSMAENMNLQRANSDTDLVTSDSRSSLTASMYEYTLGHSQNLIIFWDIKEEVDPTDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGMEGTEEGASGQHSRKLVSFTLSDIRAVGLKKGMFFNPDPYLKMSIQPGKKSTFPTFAHHGQERRSTIISNTTNPVWHREKYSFVALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERHAIGDQILSYNLGRRLPADHVSGYLQFKVEITSSIHEDASPEAVGTLLGVNSVNGDLGSPSDDEDMPAGHHDTHPVCSNGSVLEESSGEAIPKHHFRTSTALETDQEELTSSTSRSSPTRGRQDSLNDYLDAIEHSSHPRPGTSGGSERSLGASPKLRSSFPTDTRLNAMLHIDSDEEEHELHQDMGFQSSLEEEGGLVMLNGATRNVEEHCSSHSSNQVMQGSADNGKVLSFETPLQPSDDQQETLQRLPPSDVAIEGENQQDCQNEVPVDQAGIELCACQEAEQPTSSADTGAIDSAVGNRRGISETESIDQGSEPSQVSSETEHSDPARTESVSEASTRPEGESDVEGADSSCNESVTTRLSSVETRCSSFESARFPETPAFSSQEEEEGACATHVARSEPAAETQETMRCPGSLPVVRLPSTQEEVQLAEGGSLQDGEEAEEIWQRRRSLQAAAAAAAAASSQSQDEETGDAQEACEGAAAPIEGATGGSQANGHQALRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQILQRSNSIQQMEQLNRRYQSIRRTMTNDRPEEHANTVDGAGEETDLHPSNADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFPVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHSGPPVLPRPSSTFNTVSRAQYQDIVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWATVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_019492109.1
Organism	Alligator mississippiensis(American alligator)
Definition	Alligator mississippiensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019492109.1

ORF Insert Sequence:

ATGGCCAGTT CGGCCCGGGA CCACCTACTT TTTGTGCGAC GTCGCAACCC CCAACTGCGG 
TATACTTTGA GCCCAGAGAA CCTGCAGAGT CTGGCATCTC AGGGCTCCAT GGCTGAGAAT 
ATGAATTTAC AGCGTGCCAA CAGTGACACT GATCTAGTGA CCTCTGATAG CCGCTCCAGT 
CTGACAGCTA GTATGTATGA GTATACCCTT GGGCACTCTC AGAATCTCAT TATCTTCTGG 
GATATTAAAG AAGAAGTGGA CCCAACAGAT TGGATAGGAC TTTATCATAT AGATGAGAAC 
TCTCCAGCTA ATTTCTGGGA TTCCAAGAAC CGGGGAGTGA CTGGCACACA AAAAGGGCAA 
ATTGTATGGC GAATTGAACC TGGCCCTTAT TTTATGGAAC CTGAAATAAA GATTTGTTTT 
AAATACTACC ATGGCATTAG TGGAGCCCTC CGAGCTACTA CCCCATGTAT CACTGTGAAA 
AATCCTGCTG TGATGATGGG GATGGAAGGC ACAGAGGAAG GTGCTTCTGG TCAGCATTCT 
CGGAAATTAG TCAGCTTTAC ACTTTCAGAT ATTAGGGCAG TTGGGCTAAA AAAGGGGATG 
TTCTTCAACC CTGATCCTTA CCTAAAGATG TCGATTCAAC CAGGGAAGAA AAGTACATTC 
CCTACGTTTG CCCATCATGG TCAGGAAAGA CGGTCTACTA TCATCAGTAA CACAACTAAT 
CCTGTTTGGC ACAGAGAGAA ATATTCTTTT GTTGCACTTC TAACAGATGT CTTGGAGATT 
GAAATTAAAG ACAAATTTGC CAAGAGTCGT CCTATCATCA AGCGCTTTCT TGGGAAGTTA 
ACCATCCCAG TCCAGAGGCT ATTGGAGAGA CATGCAATAG GAGACCAAAT TCTCAGCTAC 
AATCTTGGCA GAAGGCTCCC AGCTGACCAT GTAAGTGGGT ATCTGCAGTT CAAAGTGGAG 
ATTACATCTT CTATTCATGA AGATGCTTCA CCAGAAGCAG TTGGTACTTT GCTAGGAGTG 
AATTCCGTAA ATGGAGATTT GGGAAGCCCC TCTGATGATG AGGACATGCC AGCCGGACAT 
CATGACACAC ATCCAGTTTG TTCAAATGGT TCAGTTCTTG AGGAATCTTC TGGAGAGGCA 
ATACCAAAGC ATCATTTCAG GACTAGTACG GCTCTAGAAA CAGACCAGGA GGAATTAACT 
TCTTCTACTT CAAGGTCATC ACCTACCAGA GGTCGCCAGG ACTCACTGAA TGACTATCTG 
GATGCAATAG AACACAGCAG TCATCCGAGA CCTGGAACAT CTGGTGGCAG TGAGCGTTCA 
CTTGGGGCCT CCCCAAAACT AAGAAGTAGC TTTCCCACTG ACACAAGACT TAATGCTATG 
CTCCACATTG ATTCAGATGA AGAGGAGCAT GAGTTGCATC AAGATATGGG TTTTCAGTCT 
TCCTTGGAAG AAGAAGGTGG TTTAGTGATG CTTAATGGTG CTACAAGGAA TGTTGAAGAA 
CATTGTTCAA GTCACTCATC AAATCAGGTA ATGCAGGGTT CAGCAGACAA TGGAAAAGTT 
TTATCTTTTG AAACTCCTTT GCAGCCATCT GATGACCAGC AAGAGACTCT CCAAAGGCTT 
CCACCATCAG ACGTGGCAAT TGAAGGTGAA AACCAGCAAG ACTGTCAAAA TGAAGTGCCA 
GTAGACCAGG CTGGCATTGA ATTGTGTGCC TGTCAGGAGG CAGAACAGCC TACTAGCAGT 
GCTGATACAG GAGCTATTGA CTCTGCTGTT GGTAATAGAA GAGGTATTAG TGAAACAGAA 
TCTATTGACC AAGGGTCTGA ACCTTCTCAG GTGTCATCAG AAACTGAGCA CAGTGACCCT 
GCTCGCACAG AGAGTGTTAG TGAGGCTAGT ACTAGGCCAG AAGGGGAGAG TGATGTTGAA 
GGAGCAGACA GCTCTTGTAA TGAAAGTGTG ACCACACGGC TGTCGTCAGT TGAAACACGA 
TGTTCATCTT TTGAAAGTGC CAGGTTTCCT GAGACTCCTG CCTTTTCATC TCAGGAAGAG 
GAGGAGGGTG CTTGTGCCAC TCATGTGGCT AGAAGTGAGC CTGCTGCAGA AACACAGGAG 
ACTATGCGCT GTCCTGGCTC TTTACCTGTA GTGAGATTAC CAAGCACTCA AGAGGAGGTG 
CAGCTGGCTG AAGGAGGTAG TTTACAGGAT GGAGAGGAAG CAGAAGAGAT TTGGCAAAGA 
AGGAGGAGCC TGCAGGCTGC TGCTGCTGCT GCTGCTGCTG CAAGCAGCCA GTCCCAGGAT 
GAAGAAACTG GAGATGCCCA GGAAGCTTGT GAGGGAGCAG CTGCACCAAT TGAAGGAGCT 
ACTGGAGGTT CTCAAGCAAA TGGCCACCAA GCTTTGAGGT CACTACCTTC GGTGCGTCAA 
GATGTCAGCC GATATCAAAG AGTGGATGAG GCTCTACCAC CAAATTGGGA GGCTCGGATC 
GACAGCCATG GAAGGATATT CTATGTGGAC CATGTAAACA GAACTACAAC ATGGCAGCGA 
CCCACAGCTC CCCCTGCCCC ACAAATCCTC CAGAGGTCAA ACTCTATTCA GCAAATGGAG 
CAGCTGAACC GCCGGTATCA GAGTATCCGA CGAACAATGA CTAATGACAG GCCTGAAGAG 
CACGCAAACA CTGTTGATGG AGCTGGAGAG GAAACTGACC TCCACCCTTC TAATGCAGAT 
TTTCGAAGGG AGAATGTGCT GCCTCACTCT ACCTCCAGGT CCAGGCTTAC TTTGTTGCTA 
CAGTCTCCTC CTGTGAAATT TCTTATCAGT CCAGAATTCT TTCCAGTGCT GCATTCTAAC 
CCTAGTGCCT ACCGCATGTT TACAAACAAC ACGTGTTTAA AGCACATGAT CACCAAAGTC 
CGGCGGGACA CCCACCATTT TGAGCGCTAC CAACATAATC GTGATCTGGT GGGCTTCCTT 
AATATGTTTG CTAACAAACA GCTGGAGCTG CCAAGAGGCT GGGAAATGAA ACATGACCAT 
CAGGGCAAGG CTTTTTTTGT TGACCACAAT TCTCGCACCA CCACATTCAT CGATCCCCGG 
CTTCCCTTGC AGAGTAGCAG GCCCACTAGT GCATTAGTTC ATCGGCAGCA TTTAACCAGA 
CAACGCAGCC ATAGTGCGGG TGAGGTAGGA GAGGACTCCC GTCATTCAGG ACCACCAGTT 
TTACCAAGAC CATCAAGCAC ATTTAATACA GTCAGTAGAG CGCAGTATCA GGACATTGTT 
CCAGTGGCCT ATAATGACAA GATTGTTGCA TTCCTGCGAC AGCCCAATAT CTTTGAAATT 
CTACAGGAGC GTCAACCAGA TCTCACCAGG AATCATTCAC TCAGGGAGAA GATTCAGTTT 
ATTCGAACAG AGGGAACCCC AGGGCTGGTG CGTCTATCCA GTGATGCAGA CCTTGTCATG 
TTACTGAGTT TGTTTGAAGA AGAGATAATG TCATATGTGC CGCCACATGC CTTACTTCAT 
CCCAGTTATT GCCAGTCCCC AAGAGGCTCC CCAGTGTCCT CACCTCAGAA CTCTCCAGGT 
ACTCAGCGTG CCAATGCCCG AGCTCCAGCT CCTTATAAAA GAGATTTTGA AGCCAAGCTG 
AGGAACTTCT ACAGGAAGCT AGAGACTAAA GGATATGGAC AAGGCCCAGG GAAACTAAAG 
TTAATCATCA GAAGAGACCA CTTGCTGGAA GATGCCTTTA ACCAGATTAT GGGTTACTCA 
AGAAAAGACC TACAGAGAAA TAAACTCTAT GTCACGTTTG TTGGGGAGGA AGGGTTGGAT 
TACAGTGGAC CTTCAAGAGA ATTTTTCTTT CTGGTGTCCA GAGAACTATT CAATCCATAT 
TATGGTTTAT TTGAATATTC AGCCAATGAT ACCTACACAG TTCAAATTAG TCCCATGTCT 
GCTTTTGTGG ACAATCACCA TGAGTGGTTC AGGTTTAGTG GTCGAATCCT TGGTCTTGCT 
CTGATACATC AATATCTGCT AGATGCCTTC TTTACCCGGC CCTTTTATAA AGCCCTCCTC 
CGAATACTAT GTGACCTGAG TGACTTGGAA TACCTTGATG AGGAGTTCCA CCAGAGTTTG 
CAGTGGATGA AAGACAACGA TATACATGAT ATCCTAGATC TCACATTCAC TGTAAATGAA 
GAAGTTTTTG GGCAGATCAC TGAACGGGAA TTAAAGCCAG GAGGTGCCAA CATCCCAGTC 
ACAGAGAAAA ACAAAAAAGA ATACATAGAG AGAATGGTGA AATGGAGAAT TGAGAGAGGG 
GTTGTGCAGC AAACAGAAAG TCTAGTTCGT GGTTTCTATG AGGTGGTTGA TGCTCGACTT 
GTGTCTGTAT TTGATGCAAG AGAACTGGAA CTGGTTATTG CTGGCACAGC AGAAATTGAC 
TTAAGTGACT GGAGAAATAA CACAGAGTAT AGAGGAGGTT ACCATGACAA TCATATTGTA 
ATTCGCTGGT TCTGGGCTAC TGTAGAAAGG TTCAACAATG AACAAAGACT AAGGCTGTTA 
CAGTTTGTTA CAGGCACCTC CAGCATACCT TATGAAGGCT TTGCCTCTCT GAGAGGGAGC 
AATGGGCCAA GAAGATTCTG TGTAGAAAAA TGGGGGAAAA TCACTGCTCT TCCAAGGGCT 
CATACTTGTT TCAATCGTCT TGATCTTCCC CCTTACCCAT CCTTCTCCAT GCTGTATGAG 
AAACTGTTGA CAGCAGTTGA AGAGACAAGT ACTTTTGGAC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_019492112.1 ) cDNA ORF clone, Alligator mississippiensis(American alligator) -> XP_019347657.1 Alligator mississippiensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X4, mRNA.

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CloneID	OAl62120
Clone ID Related Accession (Same CDS sequence)	XM_019492111.1 , XM_019492110.1 , XM_019492109.1 , XM_019492112.1
Accession Version	XM_019492112.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-08
Organism	Alligator mississippiensis(American alligator)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017711096.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator mississippiensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGTT CGGCCCGGGA CCACCTACTT TTTGTGCGAC GTCGCAACCC CCAACTGCGG 
TATACTTTGA GCCCAGAGAA CCTGCAGAGT CTGGCATCTC AGGGCTCCAT GGCTGAGAAT 
ATGAATTTAC AGCGTGCCAA CAGTGACACT GATCTAGTGA CCTCTGATAG CCGCTCCAGT 
CTGACAGCTA GTATGTATGA GTATACCCTT GGGCACTCTC AGAATCTCAT TATCTTCTGG 
GATATTAAAG AAGAAGTGGA CCCAACAGAT TGGATAGGAC TTTATCATAT AGATGAGAAC 
TCTCCAGCTA ATTTCTGGGA TTCCAAGAAC CGGGGAGTGA CTGGCACACA AAAAGGGCAA 
ATTGTATGGC GAATTGAACC TGGCCCTTAT TTTATGGAAC CTGAAATAAA GATTTGTTTT 
AAATACTACC ATGGCATTAG TGGAGCCCTC CGAGCTACTA CCCCATGTAT CACTGTGAAA 
AATCCTGCTG TGATGATGGG GATGGAAGGC ACAGAGGAAG GTGCTTCTGG TCAGCATTCT 
CGGAAATTAG TCAGCTTTAC ACTTTCAGAT ATTAGGGCAG TTGGGCTAAA AAAGGGGATG 
TTCTTCAACC CTGATCCTTA CCTAAAGATG TCGATTCAAC CAGGGAAGAA AAGTACATTC 
CCTACGTTTG CCCATCATGG TCAGGAAAGA CGGTCTACTA TCATCAGTAA CACAACTAAT 
CCTGTTTGGC ACAGAGAGAA ATATTCTTTT GTTGCACTTC TAACAGATGT CTTGGAGATT 
GAAATTAAAG ACAAATTTGC CAAGAGTCGT CCTATCATCA AGCGCTTTCT TGGGAAGTTA 
ACCATCCCAG TCCAGAGGCT ATTGGAGAGA CATGCAATAG GAGACCAAAT TCTCAGCTAC 
AATCTTGGCA GAAGGCTCCC AGCTGACCAT GTAAGTGGGT ATCTGCAGTT CAAAGTGGAG 
ATTACATCTT CTATTCATGA AGATGCTTCA CCAGAAGCAG TTGGTACTTT GCTAGGAGTG 
AATTCCGTAA ATGGAGATTT GGGAAGCCCC TCTGATGATG AGGACATGCC AGCCGGACAT 
CATGACACAC ATCCAGTTTG TTCAAATGGT TCAGTTCTTG AGGAATCTTC TGGAGAGGCA 
ATACCAAAGC ATCATTTCAG GACTAGTACG GCTCTAGAAA CAGACCAGGA GGAATTAACT 
TCTTCTACTT CAAGGTCATC ACCTACCAGA GGTCGCCAGG ACTCACTGAA TGACTATCTG 
GATGCAATAG AACACAGCAG TCATCCGAGA CCTGGAACAT CTGGTGGCAG TGAGCGTTCA 
CTTGGGGCCT CCCCAAAACT AAGAAGTAGC TTTCCCACTG ACACAAGACT TAATGCTATG 
CTCCACATTG ATTCAGATGA AGAGGAGCAT GAGTTGCATC AAGATATGGG TTTTCAGTCT 
TCCTTGGAAG AAGAAGGTGG TTTAGTGATG CTTAATGGTG CTACAAGGAA TGTTGAAGAA 
CATTGTTCAA GTCACTCATC AAATCAGGTA ATGCAGGGTT CAGCAGACAA TGGAAAAGTT 
TTATCTTTTG AAACTCCTTT GCAGCCATCT GATGACCAGC AAGAGACTCT CCAAAGGCTT 
CCACCATCAG ACGTGGCAAT TGAAGGTGAA AACCAGCAAG ACTGTCAAAA TGAAGTGCCA 
GTAGACCAGG CTGGCATTGA ATTGTGTGCC TGTCAGGAGG CAGAACAGCC TACTAGCAGT 
GCTGATACAG GAGCTATTGA CTCTGCTGTT GGTAATAGAA GAGGTATTAG TGAAACAGAA 
TCTATTGACC AAGGGTCTGA ACCTTCTCAG GTGTCATCAG AAACTGAGCA CAGTGACCCT 
GCTCGCACAG AGAGTGTTAG TGAGGCTAGT ACTAGGCCAG AAGGGGAGAG TGATGTTGAA 
GGAGCAGACA GCTCTTGTAA TGAAAGTGTG ACCACACGGC TGTCGTCAGT TGAAACACGA 
TGTTCATCTT TTGAAAGTGC CAGGTTTCCT GAGACTCCTG CCTTTTCATC TCAGGAAGAG 
GAGGAGGGTG CTTGTGCCAC TCATGTGGCT AGAAGTGAGC CTGCTGCAGA AACACAGGAG 
ACTATGCGCT GTCCTGGCTC TTTACCTGTA GTGAGATTAC CAAGCACTCA AGAGGAGGTG 
CAGCTGGCTG AAGGAGGTAG TTTACAGGAT GGAGAGGAAG CAGAAGAGAT TTGGCAAAGA 
AGGAGGAGCC TGCAGGCTGC TGCTGCTGCT GCTGCTGCTG CAAGCAGCCA GTCCCAGGAT 
GAAGAAACTG GAGATGCCCA GGAAGCTTGT GAGGGAGCAG CTGCACCAAT TGAAGGAGCT 
ACTGGAGGTT CTCAAGCAAA TGGCCACCAA GCTTTGAGGT CACTACCTTC GGTGCGTCAA 
GATGTCAGCC GATATCAAAG AGTGGATGAG GCTCTACCAC CAAATTGGGA GGCTCGGATC 
GACAGCCATG GAAGGATATT CTATGTGGAC CATGTAAACA GAACTACAAC ATGGCAGCGA 
CCCACAGCTC CCCCTGCCCC ACAAATCCTC CAGAGGTCAA ACTCTATTCA GCAAATGGAG 
CAGCTGAACC GCCGGTATCA GAGTATCCGA CGAACAATGA CTAATGACAG GCCTGAAGAG 
CACGCAAACA CTGTTGATGG AGCTGGAGAG GAAACTGACC TCCACCCTTC TAATGCAGAT 
TTTCGAAGGG AGAATGTGCT GCCTCACTCT ACCTCCAGGT CCAGGCTTAC TTTGTTGCTA 
CAGTCTCCTC CTGTGAAATT TCTTATCAGT CCAGAATTCT TTCCAGTGCT GCATTCTAAC 
CCTAGTGCCT ACCGCATGTT TACAAACAAC ACGTGTTTAA AGCACATGAT CACCAAAGTC 
CGGCGGGACA CCCACCATTT TGAGCGCTAC CAACATAATC GTGATCTGGT GGGCTTCCTT 
AATATGTTTG CTAACAAACA GCTGGAGCTG CCAAGAGGCT GGGAAATGAA ACATGACCAT 
CAGGGCAAGG CTTTTTTTGT TGACCACAAT TCTCGCACCA CCACATTCAT CGATCCCCGG 
CTTCCCTTGC AGAGTAGCAG GCCCACTAGT GCATTAGTTC ATCGGCAGCA TTTAACCAGA 
CAACGCAGCC ATAGTGCGGG TGAGGTAGGA GAGGACTCCC GTCATTCAGG ACCACCAGTT 
TTACCAAGAC CATCAAGCAC ATTTAATACA GTCAGTAGAG CGCAGTATCA GGACATTGTT 
CCAGTGGCCT ATAATGACAA GATTGTTGCA TTCCTGCGAC AGCCCAATAT CTTTGAAATT 
CTACAGGAGC GTCAACCAGA TCTCACCAGG AATCATTCAC TCAGGGAGAA GATTCAGTTT 
ATTCGAACAG AGGGAACCCC AGGGCTGGTG CGTCTATCCA GTGATGCAGA CCTTGTCATG 
TTACTGAGTT TGTTTGAAGA AGAGATAATG TCATATGTGC CGCCACATGC CTTACTTCAT 
CCCAGTTATT GCCAGTCCCC AAGAGGCTCC CCAGTGTCCT CACCTCAGAA CTCTCCAGGT 
ACTCAGCGTG CCAATGCCCG AGCTCCAGCT CCTTATAAAA GAGATTTTGA AGCCAAGCTG 
AGGAACTTCT ACAGGAAGCT AGAGACTAAA GGATATGGAC AAGGCCCAGG GAAACTAAAG 
TTAATCATCA GAAGAGACCA CTTGCTGGAA GATGCCTTTA ACCAGATTAT GGGTTACTCA 
AGAAAAGACC TACAGAGAAA TAAACTCTAT GTCACGTTTG TTGGGGAGGA AGGGTTGGAT 
TACAGTGGAC CTTCAAGAGA ATTTTTCTTT CTGGTGTCCA GAGAACTATT CAATCCATAT 
TATGGTTTAT TTGAATATTC AGCCAATGAT ACCTACACAG TTCAAATTAG TCCCATGTCT 
GCTTTTGTGG ACAATCACCA TGAGTGGTTC AGGTTTAGTG GTCGAATCCT TGGTCTTGCT 
CTGATACATC AATATCTGCT AGATGCCTTC TTTACCCGGC CCTTTTATAA AGCCCTCCTC 
CGAATACTAT GTGACCTGAG TGACTTGGAA TACCTTGATG AGGAGTTCCA CCAGAGTTTG 
CAGTGGATGA AAGACAACGA TATACATGAT ATCCTAGATC TCACATTCAC TGTAAATGAA 
GAAGTTTTTG GGCAGATCAC TGAACGGGAA TTAAAGCCAG GAGGTGCCAA CATCCCAGTC 
ACAGAGAAAA ACAAAAAAGA ATACATAGAG AGAATGGTGA AATGGAGAAT TGAGAGAGGG 
GTTGTGCAGC AAACAGAAAG TCTAGTTCGT GGTTTCTATG AGGTGGTTGA TGCTCGACTT 
GTGTCTGTAT TTGATGCAAG AGAACTGGAA CTGGTTATTG CTGGCACAGC AGAAATTGAC 
TTAAGTGACT GGAGAAATAA CACAGAGTAT AGAGGAGGTT ACCATGACAA TCATATTGTA 
ATTCGCTGGT TCTGGGCTAC TGTAGAAAGG TTCAACAATG AACAAAGACT AAGGCTGTTA 
CAGTTTGTTA CAGGCACCTC CAGCATACCT TATGAAGGCT TTGCCTCTCT GAGAGGGAGC 
AATGGGCCAA GAAGATTCTG TGTAGAAAAA TGGGGGAAAA TCACTGCTCT TCCAAGGGCT 
CATACTTGTT TCAATCGTCT TGATCTTCCC CCTTACCCAT CCTTCTCCAT GCTGTATGAG 
AAACTGTTGA CAGCAGTTGA AGAGACAAGT ACTTTTGGAC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019347657.1
CDS	691..5418
Translation	MASSARDHLLFVRRRNPQLRYTLSPENLQSLASQGSMAENMNLQRANSDTDLVTSDSRSSLTASMYEYTLGHSQNLIIFWDIKEEVDPTDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGMEGTEEGASGQHSRKLVSFTLSDIRAVGLKKGMFFNPDPYLKMSIQPGKKSTFPTFAHHGQERRSTIISNTTNPVWHREKYSFVALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERHAIGDQILSYNLGRRLPADHVSGYLQFKVEITSSIHEDASPEAVGTLLGVNSVNGDLGSPSDDEDMPAGHHDTHPVCSNGSVLEESSGEAIPKHHFRTSTALETDQEELTSSTSRSSPTRGRQDSLNDYLDAIEHSSHPRPGTSGGSERSLGASPKLRSSFPTDTRLNAMLHIDSDEEEHELHQDMGFQSSLEEEGGLVMLNGATRNVEEHCSSHSSNQVMQGSADNGKVLSFETPLQPSDDQQETLQRLPPSDVAIEGENQQDCQNEVPVDQAGIELCACQEAEQPTSSADTGAIDSAVGNRRGISETESIDQGSEPSQVSSETEHSDPARTESVSEASTRPEGESDVEGADSSCNESVTTRLSSVETRCSSFESARFPETPAFSSQEEEEGACATHVARSEPAAETQETMRCPGSLPVVRLPSTQEEVQLAEGGSLQDGEEAEEIWQRRRSLQAAAAAAAAASSQSQDEETGDAQEACEGAAAPIEGATGGSQANGHQALRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQILQRSNSIQQMEQLNRRYQSIRRTMTNDRPEEHANTVDGAGEETDLHPSNADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFPVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHSGPPVLPRPSSTFNTVSRAQYQDIVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWATVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_019492112.1
Organism	Alligator mississippiensis(American alligator)
Definition	Alligator mississippiensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019492112.1

ORF Insert Sequence:

ATGGCCAGTT CGGCCCGGGA CCACCTACTT TTTGTGCGAC GTCGCAACCC CCAACTGCGG 
TATACTTTGA GCCCAGAGAA CCTGCAGAGT CTGGCATCTC AGGGCTCCAT GGCTGAGAAT 
ATGAATTTAC AGCGTGCCAA CAGTGACACT GATCTAGTGA CCTCTGATAG CCGCTCCAGT 
CTGACAGCTA GTATGTATGA GTATACCCTT GGGCACTCTC AGAATCTCAT TATCTTCTGG 
GATATTAAAG AAGAAGTGGA CCCAACAGAT TGGATAGGAC TTTATCATAT AGATGAGAAC 
TCTCCAGCTA ATTTCTGGGA TTCCAAGAAC CGGGGAGTGA CTGGCACACA AAAAGGGCAA 
ATTGTATGGC GAATTGAACC TGGCCCTTAT TTTATGGAAC CTGAAATAAA GATTTGTTTT 
AAATACTACC ATGGCATTAG TGGAGCCCTC CGAGCTACTA CCCCATGTAT CACTGTGAAA 
AATCCTGCTG TGATGATGGG GATGGAAGGC ACAGAGGAAG GTGCTTCTGG TCAGCATTCT 
CGGAAATTAG TCAGCTTTAC ACTTTCAGAT ATTAGGGCAG TTGGGCTAAA AAAGGGGATG 
TTCTTCAACC CTGATCCTTA CCTAAAGATG TCGATTCAAC CAGGGAAGAA AAGTACATTC 
CCTACGTTTG CCCATCATGG TCAGGAAAGA CGGTCTACTA TCATCAGTAA CACAACTAAT 
CCTGTTTGGC ACAGAGAGAA ATATTCTTTT GTTGCACTTC TAACAGATGT CTTGGAGATT 
GAAATTAAAG ACAAATTTGC CAAGAGTCGT CCTATCATCA AGCGCTTTCT TGGGAAGTTA 
ACCATCCCAG TCCAGAGGCT ATTGGAGAGA CATGCAATAG GAGACCAAAT TCTCAGCTAC 
AATCTTGGCA GAAGGCTCCC AGCTGACCAT GTAAGTGGGT ATCTGCAGTT CAAAGTGGAG 
ATTACATCTT CTATTCATGA AGATGCTTCA CCAGAAGCAG TTGGTACTTT GCTAGGAGTG 
AATTCCGTAA ATGGAGATTT GGGAAGCCCC TCTGATGATG AGGACATGCC AGCCGGACAT 
CATGACACAC ATCCAGTTTG TTCAAATGGT TCAGTTCTTG AGGAATCTTC TGGAGAGGCA 
ATACCAAAGC ATCATTTCAG GACTAGTACG GCTCTAGAAA CAGACCAGGA GGAATTAACT 
TCTTCTACTT CAAGGTCATC ACCTACCAGA GGTCGCCAGG ACTCACTGAA TGACTATCTG 
GATGCAATAG AACACAGCAG TCATCCGAGA CCTGGAACAT CTGGTGGCAG TGAGCGTTCA 
CTTGGGGCCT CCCCAAAACT AAGAAGTAGC TTTCCCACTG ACACAAGACT TAATGCTATG 
CTCCACATTG ATTCAGATGA AGAGGAGCAT GAGTTGCATC AAGATATGGG TTTTCAGTCT 
TCCTTGGAAG AAGAAGGTGG TTTAGTGATG CTTAATGGTG CTACAAGGAA TGTTGAAGAA 
CATTGTTCAA GTCACTCATC AAATCAGGTA ATGCAGGGTT CAGCAGACAA TGGAAAAGTT 
TTATCTTTTG AAACTCCTTT GCAGCCATCT GATGACCAGC AAGAGACTCT CCAAAGGCTT 
CCACCATCAG ACGTGGCAAT TGAAGGTGAA AACCAGCAAG ACTGTCAAAA TGAAGTGCCA 
GTAGACCAGG CTGGCATTGA ATTGTGTGCC TGTCAGGAGG CAGAACAGCC TACTAGCAGT 
GCTGATACAG GAGCTATTGA CTCTGCTGTT GGTAATAGAA GAGGTATTAG TGAAACAGAA 
TCTATTGACC AAGGGTCTGA ACCTTCTCAG GTGTCATCAG AAACTGAGCA CAGTGACCCT 
GCTCGCACAG AGAGTGTTAG TGAGGCTAGT ACTAGGCCAG AAGGGGAGAG TGATGTTGAA 
GGAGCAGACA GCTCTTGTAA TGAAAGTGTG ACCACACGGC TGTCGTCAGT TGAAACACGA 
TGTTCATCTT TTGAAAGTGC CAGGTTTCCT GAGACTCCTG CCTTTTCATC TCAGGAAGAG 
GAGGAGGGTG CTTGTGCCAC TCATGTGGCT AGAAGTGAGC CTGCTGCAGA AACACAGGAG 
ACTATGCGCT GTCCTGGCTC TTTACCTGTA GTGAGATTAC CAAGCACTCA AGAGGAGGTG 
CAGCTGGCTG AAGGAGGTAG TTTACAGGAT GGAGAGGAAG CAGAAGAGAT TTGGCAAAGA 
AGGAGGAGCC TGCAGGCTGC TGCTGCTGCT GCTGCTGCTG CAAGCAGCCA GTCCCAGGAT 
GAAGAAACTG GAGATGCCCA GGAAGCTTGT GAGGGAGCAG CTGCACCAAT TGAAGGAGCT 
ACTGGAGGTT CTCAAGCAAA TGGCCACCAA GCTTTGAGGT CACTACCTTC GGTGCGTCAA 
GATGTCAGCC GATATCAAAG AGTGGATGAG GCTCTACCAC CAAATTGGGA GGCTCGGATC 
GACAGCCATG GAAGGATATT CTATGTGGAC CATGTAAACA GAACTACAAC ATGGCAGCGA 
CCCACAGCTC CCCCTGCCCC ACAAATCCTC CAGAGGTCAA ACTCTATTCA GCAAATGGAG 
CAGCTGAACC GCCGGTATCA GAGTATCCGA CGAACAATGA CTAATGACAG GCCTGAAGAG 
CACGCAAACA CTGTTGATGG AGCTGGAGAG GAAACTGACC TCCACCCTTC TAATGCAGAT 
TTTCGAAGGG AGAATGTGCT GCCTCACTCT ACCTCCAGGT CCAGGCTTAC TTTGTTGCTA 
CAGTCTCCTC CTGTGAAATT TCTTATCAGT CCAGAATTCT TTCCAGTGCT GCATTCTAAC 
CCTAGTGCCT ACCGCATGTT TACAAACAAC ACGTGTTTAA AGCACATGAT CACCAAAGTC 
CGGCGGGACA CCCACCATTT TGAGCGCTAC CAACATAATC GTGATCTGGT GGGCTTCCTT 
AATATGTTTG CTAACAAACA GCTGGAGCTG CCAAGAGGCT GGGAAATGAA ACATGACCAT 
CAGGGCAAGG CTTTTTTTGT TGACCACAAT TCTCGCACCA CCACATTCAT CGATCCCCGG 
CTTCCCTTGC AGAGTAGCAG GCCCACTAGT GCATTAGTTC ATCGGCAGCA TTTAACCAGA 
CAACGCAGCC ATAGTGCGGG TGAGGTAGGA GAGGACTCCC GTCATTCAGG ACCACCAGTT 
TTACCAAGAC CATCAAGCAC ATTTAATACA GTCAGTAGAG CGCAGTATCA GGACATTGTT 
CCAGTGGCCT ATAATGACAA GATTGTTGCA TTCCTGCGAC AGCCCAATAT CTTTGAAATT 
CTACAGGAGC GTCAACCAGA TCTCACCAGG AATCATTCAC TCAGGGAGAA GATTCAGTTT 
ATTCGAACAG AGGGAACCCC AGGGCTGGTG CGTCTATCCA GTGATGCAGA CCTTGTCATG 
TTACTGAGTT TGTTTGAAGA AGAGATAATG TCATATGTGC CGCCACATGC CTTACTTCAT 
CCCAGTTATT GCCAGTCCCC AAGAGGCTCC CCAGTGTCCT CACCTCAGAA CTCTCCAGGT 
ACTCAGCGTG CCAATGCCCG AGCTCCAGCT CCTTATAAAA GAGATTTTGA AGCCAAGCTG 
AGGAACTTCT ACAGGAAGCT AGAGACTAAA GGATATGGAC AAGGCCCAGG GAAACTAAAG 
TTAATCATCA GAAGAGACCA CTTGCTGGAA GATGCCTTTA ACCAGATTAT GGGTTACTCA 
AGAAAAGACC TACAGAGAAA TAAACTCTAT GTCACGTTTG TTGGGGAGGA AGGGTTGGAT 
TACAGTGGAC CTTCAAGAGA ATTTTTCTTT CTGGTGTCCA GAGAACTATT CAATCCATAT 
TATGGTTTAT TTGAATATTC AGCCAATGAT ACCTACACAG TTCAAATTAG TCCCATGTCT 
GCTTTTGTGG ACAATCACCA TGAGTGGTTC AGGTTTAGTG GTCGAATCCT TGGTCTTGCT 
CTGATACATC AATATCTGCT AGATGCCTTC TTTACCCGGC CCTTTTATAA AGCCCTCCTC 
CGAATACTAT GTGACCTGAG TGACTTGGAA TACCTTGATG AGGAGTTCCA CCAGAGTTTG 
CAGTGGATGA AAGACAACGA TATACATGAT ATCCTAGATC TCACATTCAC TGTAAATGAA 
GAAGTTTTTG GGCAGATCAC TGAACGGGAA TTAAAGCCAG GAGGTGCCAA CATCCCAGTC 
ACAGAGAAAA ACAAAAAAGA ATACATAGAG AGAATGGTGA AATGGAGAAT TGAGAGAGGG 
GTTGTGCAGC AAACAGAAAG TCTAGTTCGT GGTTTCTATG AGGTGGTTGA TGCTCGACTT 
GTGTCTGTAT TTGATGCAAG AGAACTGGAA CTGGTTATTG CTGGCACAGC AGAAATTGAC 
TTAAGTGACT GGAGAAATAA CACAGAGTAT AGAGGAGGTT ACCATGACAA TCATATTGTA 
ATTCGCTGGT TCTGGGCTAC TGTAGAAAGG TTCAACAATG AACAAAGACT AAGGCTGTTA 
CAGTTTGTTA CAGGCACCTC CAGCATACCT TATGAAGGCT TTGCCTCTCT GAGAGGGAGC 
AATGGGCCAA GAAGATTCTG TGTAGAAAAA TGGGGGAAAA TCACTGCTCT TCCAAGGGCT 
CATACTTGTT TCAATCGTCT TGATCTTCCC CCTTACCCAT CCTTCTCCAT GCTGTATGAG 
AAACTGTTGA CAGCAGTTGA AGAGACAAGT ACTTTTGGAC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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HECW2 cDNA ORF clone, Alligator mississippiensis(American alligator)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム