HEPH cDNA ORF clone, Alligator mississippiensis(American alligator)

Gene
Clones
gRNAs

Summary

Gene Symbol	HEPH
Entrez Gene ID	102576664
Full Name	hephaestin
Gene Type	protein-coding
Organism	Alligator mississippiensis(American alligator)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019487872.1	XP_019343417.1	hephaestin
XM_019487873.1	XP_019343418.1	hephaestin

Pathways from BioSystems
Gene Ontology

The following HEPH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HEPH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAl57099	XM_019487872.1 Latest version!	Alligator mississippiensis hephaestin (HEPH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAl57099	XM_019487873.1 Latest version!	Alligator mississippiensis hephaestin (HEPH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HEPH ( XM_019487872.1 ) cDNA ORF clone, Alligator mississippiensis(American alligator) -> XP_019343417.1 Alligator mississippiensis hephaestin (HEPH), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAl57099
Clone ID Related Accession (Same CDS sequence)	XM_019487872.1 , XM_019487873.1
Accession Version	XM_019487872.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-08
Organism	Alligator mississippiensis(American alligator)
Product	hephaestin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017709938.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator mississippiensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCT GGTGGCAACT GCTACAGTCC TTGCTTTACA TCCATGTGCT GCTGCCACTC 
TTTGCTGAAG GTGTCACCAG AGTCTATTAC CTGGGAATCC ACGAGGTGAA ATGGAACTAT 
GCTCCAACAG GGAAGAACAT AATCAGCGAC CTAAACATTA CAGCTGACTT GGAAGCCTCT 
GCATTCCTCC AGCCTGGCAA GGACAGGGTA GGAAGCATAT ACAAAAAATC TGTCTATAAG 
CAATACTCAG ACTCCACGTA CACAACTGAG ATCAGCAAAC CTGCCTGGCT GGGGTTTCTT 
GGGCCCATCA TCCGAGCTGA AGTGAGCGAT ACCATTCTTG TGCACCTAAA AAATTTTGCC 
AGTCGGCCAT ACACGATCCA TCCTCATGGC GTGTTCTATG AAAAGGACTC AGAAGGGTCC 
CAGTACCCCG ATGTGTCCTC ACCGGAGCAT AAGAAGGATG ATTCCATTCC ACCAGGAGGG 
AATCATACAT ATACTTGGAC TATACCTGAA GGTCATAGCC CAACAGCTGA TGACACAAAC 
TGCTTGACCT GGATCTACCA CTCTCATATT GATGCACCAA AGGATGTTGC TTCTGGGTTA 
ATTGGGCCAT TACTCACATG CAAAGAAGGA GTGCTAACTG GCACCTCCCA AAGGAGGCAT 
GACATGGACC TTGATTTCTT TTTGATGTTC AGTGTCATAG ATGAGAACCT GAGCTGGTAC 
TTGGATGAGA ATATTGCTTC TTTCTGCACT GACCCAGGCT CTGTCAACAA GGAAGATGAG 
GAGTTTCAAG CGAGCAACAG AATGCATGCT ATTAATGGTT ACTTGTTTGG CAACCTGCCA 
GATCTGACAA TGTGTGCTGG GGATCAGGTA GCATGGCACC TCTTTGGGAT GGGCAATGAG 
ATTGACATCC ACACAGCCTT CTTCCATGGA CAGATGCTCA CCATCAGAGA CCACAAGACT 
GATGTGGCCA GCCTTTTCCC AGCTACTTTT GTGACAGCCC ACATGATTGC CATTAACCCT 
GGGAAATGGC TTCTGAGCTG TCCGGTCAAT GATCACTTCA AAGCTGGAAT GGAGGCACTC 
TATGAAGTCA GACATTGTTC TCAGAAAACT CCGGAGTCAG CCCTTACAGG GAAAGTCCGG 
ACATACTACA TAGCTGCAAA GGAAGAACAG TGGAATTATG GACCCTCAGG ACTTGACCTG 
AGCACTGAGG AGAGCTTGCA GGAGCCAGGG AGCTCTGCTG AGCAGTTCTT CAAGCGGGGC 
CCCGGTCGGA TTGGGGGAAC TTACTGGAAG GCCAGATACA CGGAATACAC TGATGCAACT 
TTCCGGGTGG AAAAAGAGCG ATTGAAAGAA GAGAAGCATC TTGGGATACT TGGTCCAGTG 
ATAAAAGCTG AAGTGGGAGA CACCATCTTG GTGATGTTTG CTAACAAAGC CTCCTGGCCC 
TTCAGCATTC AGCCCCATGG AGTACTTTAT GGGAAGGAAT GGGAAGGAGC TATCTATCAC 
AATGGCCTGT CATGGAATGG AGTCTCTGTT AAACCTCTCC ATAATTTCAC GTATCGCTGG 
ACAGTACCAC AGCATGTTGG ACCCACAGCC AGTGACCCTC CCTGTTTAAC CTGGATGTAC 
TCCTCTGCTG TGAATCCTTA CAGAGACACT AACTCAGGCT TAGTTGGCCC CCTATTGATC 
TGCAAACCTG GAACTCTGAG TGAGAACAAC AAACAGAAAG GGATCGACAA GGAGTTTTAC 
CTTCTCTTCA GTGTGTTTGA TGAGAATCTC AGCTGGTATT TAAGTGCAAA CATAAAGTAC 
CATTTGAAAG TGGAAGAGGC CTCTGTAGAA AAGAATGAAG CTTTCGAGGA GTCCAACAGA 
ATGCATGCCA TTAATGGATT TATGTTCTCT AACATGCCCA AGCTGGAGGT ATGTAAAGGA 
GACACTGTAG CCTGGCACCT GATGGGCCTG GGAACTGAGG CAGATGTGCA TGGAGCTGTG 
TTCCAGGGAA ATACCTTTCA AATTAATGGG ATGAGAAGAG ATACAGTCAA TCTCTTCCCT 
CACACATTTG CCACTGCCTT CATGCAGCCT GATAACAAAG GGAATTTTGA GATCTATTGC 
CAAACAACCA ACCACTATCT GTCTGGGATG AGGGAGCAGT ACTTTGTTTC TGAATGCGGC 
AAAAAGATTC CTGCTCTTGC CCAGCGCTAT GAAGGAGTGA GGACATACTA CATCACGGCA 
GAGGAGGTGG AGTGGGATTA TGCACCAGAT CGTAGCTGGG AGCTGAAACG ATACAACAAT 
TCTGGGAAGG AAAGCTATGG CAACATATTT CTGAGCAATG AGAATGGACT CCTTGGCTCC 
AAGTACAAGA AAGCAGTTTA CAGGGAGTAC ACTGATGGGA CATTCAAGAT ACTCAAGAAC 
AGGAGCAGTG ATGATGAGCA CTTAGGGATC TTGGGTCCTT TTATCTGGGC AGAAGTTGGA 
GATGTCCTCA ATATTGTGTT TAAGAACAGT GCTACCCGCC CCTACTCCAT TCATGCACAT 
GGGGTACTGG AAAAGAATGA TGGACAGCTG GAAGCTGCAA TGCCTGGTGA AATTGTAACA 
TACCGGTGGG ACATCCCGGA AAGGTCTGGC CCAGGATCCA ATGATTCTGC CTGTGTTTCG 
TGGGTCTACT ACTCCATGGT AGACCCAGTA AAGGATATGT ACAGTGGCCT GATTGGCCCC 
CTAAAGGTCT GCCGAAAAGG GATACTGCAC CCTAATGGGA AAAGGAAGGA CATGAACAAG 
GAGTTTGCTC TCCTTTTCCT GGTTTTTGAT GAAAATCAGT CCTGGTATCT GGAGGAAAAT 
GTGGAAACTC ATGGGGATCA GAGCAAGATC AACCTGGAAG ATGAACGATT TATAGAGAGC 
AATAAATTGC ACGCCATAAA CGGTAGGGTC TATGCAAACC TGCCTGGGCT GACTATGTAC 
CAAGGAGACT GGGTGAGCTG GTACCTACTG GGAATGGGCC AAGAGATTGA TGTGCACACA 
GTCCATTTTC ATGCCGAAAG CTTCACACAG AGGTATGGCA AGATGTACAG AGCGGATGTT 
GTGGATCTGT TTCCTGGGAC TTTTGTGATG GTGGAGATGC TGGTTGGGAA CCCTGGGACT 
TGGCTGCTTC ATTGCCATGT TTCAGATCAT ATCCACGCTG GTATGGAAAT ACTTTTCCAC 
GTCTTACACA AACCAGAGCC AGTGGCAAAA GTCAAACAGG CTGGAGGAGT GACAACTCCT 
CCGGATGATG AGTCAAAGTA TTTCAGTATG TTTGGATATA AACTGGATCC AGGGCAAGTG 
CAGACCACAA TCATCATTCT GACCGTTGCA GGAATTGTAC TGTTCCTCAC TGCCAGCTTC 
CTGCTGGGAG TGATCATCCG TCTTGAAAGG CAAAAAAAAT TAAGACGCAA CAGGAGATCC 
ATACTGGATG ATGGCTTCAA ACTCATGTCT CAAAAGAATC AAGTGATTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019343417.1
CDS	155..3625
Translation	MGSWWQLLQSLLYIHVLLPLFAEGVTRVYYLGIHEVKWNYAPTGKNIISDLNITADLEASAFLQPGKDRVGSIYKKSVYKQYSDSTYTTEISKPAWLGFLGPIIRAEVSDTILVHLKNFASRPYTIHPHGVFYEKDSEGSQYPDVSSPEHKKDDSIPPGGNHTYTWTIPEGHSPTADDTNCLTWIYHSHIDAPKDVASGLIGPLLTCKEGVLTGTSQRRHDMDLDFFLMFSVIDENLSWYLDENIASFCTDPGSVNKEDEEFQASNRMHAINGYLFGNLPDLTMCAGDQVAWHLFGMGNEIDIHTAFFHGQMLTIRDHKTDVASLFPATFVTAHMIAINPGKWLLSCPVNDHFKAGMEALYEVRHCSQKTPESALTGKVRTYYIAAKEEQWNYGPSGLDLSTEESLQEPGSSAEQFFKRGPGRIGGTYWKARYTEYTDATFRVEKERLKEEKHLGILGPVIKAEVGDTILVMFANKASWPFSIQPHGVLYGKEWEGAIYHNGLSWNGVSVKPLHNFTYRWTVPQHVGPTASDPPCLTWMYSSAVNPYRDTNSGLVGPLLICKPGTLSENNKQKGIDKEFYLLFSVFDENLSWYLSANIKYHLKVEEASVEKNEAFEESNRMHAINGFMFSNMPKLEVCKGDTVAWHLMGLGTEADVHGAVFQGNTFQINGMRRDTVNLFPHTFATAFMQPDNKGNFEIYCQTTNHYLSGMREQYFVSECGKKIPALAQRYEGVRTYYITAEEVEWDYAPDRSWELKRYNNSGKESYGNIFLSNENGLLGSKYKKAVYREYTDGTFKILKNRSSDDEHLGILGPFIWAEVGDVLNIVFKNSATRPYSIHAHGVLEKNDGQLEAAMPGEIVTYRWDIPERSGPGSNDSACVSWVYYSMVDPVKDMYSGLIGPLKVCRKGILHPNGKRKDMNKEFALLFLVFDENQSWYLEENVETHGDQSKINLEDERFIESNKLHAINGRVYANLPGLTMYQGDWVSWYLLGMGQEIDVHTVHFHAESFTQRYGKMYRADVVDLFPGTFVMVEMLVGNPGTWLLHCHVSDHIHAGMEILFHVLHKPEPVAKVKQAGGVTTPPDDESKYFSMFGYKLDPGQVQTTIIILTVAGIVLFLTASFLLGVIIRLERQKKLRRNRRSILDDGFKLMSQKNQVI

Target ORF information:

RefSeq Version	XM_019487872.1
Organism	Alligator mississippiensis(American alligator)
Definition	Alligator mississippiensis hephaestin (HEPH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019487872.1

ORF Insert Sequence:

ATGGGTTCCT GGTGGCAACT GCTACAGTCC TTGCTTTACA TCCATGTGCT GCTGCCACTC 
TTTGCTGAAG GTGTCACCAG AGTCTATTAC CTGGGAATCC ACGAGGTGAA ATGGAACTAT 
GCTCCAACAG GGAAGAACAT AATCAGCGAC CTAAACATTA CAGCTGACTT GGAAGCCTCT 
GCATTCCTCC AGCCTGGCAA GGACAGGGTA GGAAGCATAT ACAAAAAATC TGTCTATAAG 
CAATACTCAG ACTCCACGTA CACAACTGAG ATCAGCAAAC CTGCCTGGCT GGGGTTTCTT 
GGGCCCATCA TCCGAGCTGA AGTGAGCGAT ACCATTCTTG TGCACCTAAA AAATTTTGCC 
AGTCGGCCAT ACACGATCCA TCCTCATGGC GTGTTCTATG AAAAGGACTC AGAAGGGTCC 
CAGTACCCCG ATGTGTCCTC ACCGGAGCAT AAGAAGGATG ATTCCATTCC ACCAGGAGGG 
AATCATACAT ATACTTGGAC TATACCTGAA GGTCATAGCC CAACAGCTGA TGACACAAAC 
TGCTTGACCT GGATCTACCA CTCTCATATT GATGCACCAA AGGATGTTGC TTCTGGGTTA 
ATTGGGCCAT TACTCACATG CAAAGAAGGA GTGCTAACTG GCACCTCCCA AAGGAGGCAT 
GACATGGACC TTGATTTCTT TTTGATGTTC AGTGTCATAG ATGAGAACCT GAGCTGGTAC 
TTGGATGAGA ATATTGCTTC TTTCTGCACT GACCCAGGCT CTGTCAACAA GGAAGATGAG 
GAGTTTCAAG CGAGCAACAG AATGCATGCT ATTAATGGTT ACTTGTTTGG CAACCTGCCA 
GATCTGACAA TGTGTGCTGG GGATCAGGTA GCATGGCACC TCTTTGGGAT GGGCAATGAG 
ATTGACATCC ACACAGCCTT CTTCCATGGA CAGATGCTCA CCATCAGAGA CCACAAGACT 
GATGTGGCCA GCCTTTTCCC AGCTACTTTT GTGACAGCCC ACATGATTGC CATTAACCCT 
GGGAAATGGC TTCTGAGCTG TCCGGTCAAT GATCACTTCA AAGCTGGAAT GGAGGCACTC 
TATGAAGTCA GACATTGTTC TCAGAAAACT CCGGAGTCAG CCCTTACAGG GAAAGTCCGG 
ACATACTACA TAGCTGCAAA GGAAGAACAG TGGAATTATG GACCCTCAGG ACTTGACCTG 
AGCACTGAGG AGAGCTTGCA GGAGCCAGGG AGCTCTGCTG AGCAGTTCTT CAAGCGGGGC 
CCCGGTCGGA TTGGGGGAAC TTACTGGAAG GCCAGATACA CGGAATACAC TGATGCAACT 
TTCCGGGTGG AAAAAGAGCG ATTGAAAGAA GAGAAGCATC TTGGGATACT TGGTCCAGTG 
ATAAAAGCTG AAGTGGGAGA CACCATCTTG GTGATGTTTG CTAACAAAGC CTCCTGGCCC 
TTCAGCATTC AGCCCCATGG AGTACTTTAT GGGAAGGAAT GGGAAGGAGC TATCTATCAC 
AATGGCCTGT CATGGAATGG AGTCTCTGTT AAACCTCTCC ATAATTTCAC GTATCGCTGG 
ACAGTACCAC AGCATGTTGG ACCCACAGCC AGTGACCCTC CCTGTTTAAC CTGGATGTAC 
TCCTCTGCTG TGAATCCTTA CAGAGACACT AACTCAGGCT TAGTTGGCCC CCTATTGATC 
TGCAAACCTG GAACTCTGAG TGAGAACAAC AAACAGAAAG GGATCGACAA GGAGTTTTAC 
CTTCTCTTCA GTGTGTTTGA TGAGAATCTC AGCTGGTATT TAAGTGCAAA CATAAAGTAC 
CATTTGAAAG TGGAAGAGGC CTCTGTAGAA AAGAATGAAG CTTTCGAGGA GTCCAACAGA 
ATGCATGCCA TTAATGGATT TATGTTCTCT AACATGCCCA AGCTGGAGGT ATGTAAAGGA 
GACACTGTAG CCTGGCACCT GATGGGCCTG GGAACTGAGG CAGATGTGCA TGGAGCTGTG 
TTCCAGGGAA ATACCTTTCA AATTAATGGG ATGAGAAGAG ATACAGTCAA TCTCTTCCCT 
CACACATTTG CCACTGCCTT CATGCAGCCT GATAACAAAG GGAATTTTGA GATCTATTGC 
CAAACAACCA ACCACTATCT GTCTGGGATG AGGGAGCAGT ACTTTGTTTC TGAATGCGGC 
AAAAAGATTC CTGCTCTTGC CCAGCGCTAT GAAGGAGTGA GGACATACTA CATCACGGCA 
GAGGAGGTGG AGTGGGATTA TGCACCAGAT CGTAGCTGGG AGCTGAAACG ATACAACAAT 
TCTGGGAAGG AAAGCTATGG CAACATATTT CTGAGCAATG AGAATGGACT CCTTGGCTCC 
AAGTACAAGA AAGCAGTTTA CAGGGAGTAC ACTGATGGGA CATTCAAGAT ACTCAAGAAC 
AGGAGCAGTG ATGATGAGCA CTTAGGGATC TTGGGTCCTT TTATCTGGGC AGAAGTTGGA 
GATGTCCTCA ATATTGTGTT TAAGAACAGT GCTACCCGCC CCTACTCCAT TCATGCACAT 
GGGGTACTGG AAAAGAATGA TGGACAGCTG GAAGCTGCAA TGCCTGGTGA AATTGTAACA 
TACCGGTGGG ACATCCCGGA AAGGTCTGGC CCAGGATCCA ATGATTCTGC CTGTGTTTCG 
TGGGTCTACT ACTCCATGGT AGACCCAGTA AAGGATATGT ACAGTGGCCT GATTGGCCCC 
CTAAAGGTCT GCCGAAAAGG GATACTGCAC CCTAATGGGA AAAGGAAGGA CATGAACAAG 
GAGTTTGCTC TCCTTTTCCT GGTTTTTGAT GAAAATCAGT CCTGGTATCT GGAGGAAAAT 
GTGGAAACTC ATGGGGATCA GAGCAAGATC AACCTGGAAG ATGAACGATT TATAGAGAGC 
AATAAATTGC ACGCCATAAA CGGTAGGGTC TATGCAAACC TGCCTGGGCT GACTATGTAC 
CAAGGAGACT GGGTGAGCTG GTACCTACTG GGAATGGGCC AAGAGATTGA TGTGCACACA 
GTCCATTTTC ATGCCGAAAG CTTCACACAG AGGTATGGCA AGATGTACAG AGCGGATGTT 
GTGGATCTGT TTCCTGGGAC TTTTGTGATG GTGGAGATGC TGGTTGGGAA CCCTGGGACT 
TGGCTGCTTC ATTGCCATGT TTCAGATCAT ATCCACGCTG GTATGGAAAT ACTTTTCCAC 
GTCTTACACA AACCAGAGCC AGTGGCAAAA GTCAAACAGG CTGGAGGAGT GACAACTCCT 
CCGGATGATG AGTCAAAGTA TTTCAGTATG TTTGGATATA AACTGGATCC AGGGCAAGTG 
CAGACCACAA TCATCATTCT GACCGTTGCA GGAATTGTAC TGTTCCTCAC TGCCAGCTTC 
CTGCTGGGAG TGATCATCCG TCTTGAAAGG CAAAAAAAAT TAAGACGCAA CAGGAGATCC 
ATACTGGATG ATGGCTTCAA ACTCATGTCT CAAAAGAATC AAGTGATTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_019487873.1 ) cDNA ORF clone, Alligator mississippiensis(American alligator) -> XP_019343418.1 Alligator mississippiensis hephaestin (HEPH), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAl57099
Clone ID Related Accession (Same CDS sequence)	XM_019487872.1 , XM_019487873.1
Accession Version	XM_019487873.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-08
Organism	Alligator mississippiensis(American alligator)
Product	hephaestin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017709938.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator mississippiensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCT GGTGGCAACT GCTACAGTCC TTGCTTTACA TCCATGTGCT GCTGCCACTC 
TTTGCTGAAG GTGTCACCAG AGTCTATTAC CTGGGAATCC ACGAGGTGAA ATGGAACTAT 
GCTCCAACAG GGAAGAACAT AATCAGCGAC CTAAACATTA CAGCTGACTT GGAAGCCTCT 
GCATTCCTCC AGCCTGGCAA GGACAGGGTA GGAAGCATAT ACAAAAAATC TGTCTATAAG 
CAATACTCAG ACTCCACGTA CACAACTGAG ATCAGCAAAC CTGCCTGGCT GGGGTTTCTT 
GGGCCCATCA TCCGAGCTGA AGTGAGCGAT ACCATTCTTG TGCACCTAAA AAATTTTGCC 
AGTCGGCCAT ACACGATCCA TCCTCATGGC GTGTTCTATG AAAAGGACTC AGAAGGGTCC 
CAGTACCCCG ATGTGTCCTC ACCGGAGCAT AAGAAGGATG ATTCCATTCC ACCAGGAGGG 
AATCATACAT ATACTTGGAC TATACCTGAA GGTCATAGCC CAACAGCTGA TGACACAAAC 
TGCTTGACCT GGATCTACCA CTCTCATATT GATGCACCAA AGGATGTTGC TTCTGGGTTA 
ATTGGGCCAT TACTCACATG CAAAGAAGGA GTGCTAACTG GCACCTCCCA AAGGAGGCAT 
GACATGGACC TTGATTTCTT TTTGATGTTC AGTGTCATAG ATGAGAACCT GAGCTGGTAC 
TTGGATGAGA ATATTGCTTC TTTCTGCACT GACCCAGGCT CTGTCAACAA GGAAGATGAG 
GAGTTTCAAG CGAGCAACAG AATGCATGCT ATTAATGGTT ACTTGTTTGG CAACCTGCCA 
GATCTGACAA TGTGTGCTGG GGATCAGGTA GCATGGCACC TCTTTGGGAT GGGCAATGAG 
ATTGACATCC ACACAGCCTT CTTCCATGGA CAGATGCTCA CCATCAGAGA CCACAAGACT 
GATGTGGCCA GCCTTTTCCC AGCTACTTTT GTGACAGCCC ACATGATTGC CATTAACCCT 
GGGAAATGGC TTCTGAGCTG TCCGGTCAAT GATCACTTCA AAGCTGGAAT GGAGGCACTC 
TATGAAGTCA GACATTGTTC TCAGAAAACT CCGGAGTCAG CCCTTACAGG GAAAGTCCGG 
ACATACTACA TAGCTGCAAA GGAAGAACAG TGGAATTATG GACCCTCAGG ACTTGACCTG 
AGCACTGAGG AGAGCTTGCA GGAGCCAGGG AGCTCTGCTG AGCAGTTCTT CAAGCGGGGC 
CCCGGTCGGA TTGGGGGAAC TTACTGGAAG GCCAGATACA CGGAATACAC TGATGCAACT 
TTCCGGGTGG AAAAAGAGCG ATTGAAAGAA GAGAAGCATC TTGGGATACT TGGTCCAGTG 
ATAAAAGCTG AAGTGGGAGA CACCATCTTG GTGATGTTTG CTAACAAAGC CTCCTGGCCC 
TTCAGCATTC AGCCCCATGG AGTACTTTAT GGGAAGGAAT GGGAAGGAGC TATCTATCAC 
AATGGCCTGT CATGGAATGG AGTCTCTGTT AAACCTCTCC ATAATTTCAC GTATCGCTGG 
ACAGTACCAC AGCATGTTGG ACCCACAGCC AGTGACCCTC CCTGTTTAAC CTGGATGTAC 
TCCTCTGCTG TGAATCCTTA CAGAGACACT AACTCAGGCT TAGTTGGCCC CCTATTGATC 
TGCAAACCTG GAACTCTGAG TGAGAACAAC AAACAGAAAG GGATCGACAA GGAGTTTTAC 
CTTCTCTTCA GTGTGTTTGA TGAGAATCTC AGCTGGTATT TAAGTGCAAA CATAAAGTAC 
CATTTGAAAG TGGAAGAGGC CTCTGTAGAA AAGAATGAAG CTTTCGAGGA GTCCAACAGA 
ATGCATGCCA TTAATGGATT TATGTTCTCT AACATGCCCA AGCTGGAGGT ATGTAAAGGA 
GACACTGTAG CCTGGCACCT GATGGGCCTG GGAACTGAGG CAGATGTGCA TGGAGCTGTG 
TTCCAGGGAA ATACCTTTCA AATTAATGGG ATGAGAAGAG ATACAGTCAA TCTCTTCCCT 
CACACATTTG CCACTGCCTT CATGCAGCCT GATAACAAAG GGAATTTTGA GATCTATTGC 
CAAACAACCA ACCACTATCT GTCTGGGATG AGGGAGCAGT ACTTTGTTTC TGAATGCGGC 
AAAAAGATTC CTGCTCTTGC CCAGCGCTAT GAAGGAGTGA GGACATACTA CATCACGGCA 
GAGGAGGTGG AGTGGGATTA TGCACCAGAT CGTAGCTGGG AGCTGAAACG ATACAACAAT 
TCTGGGAAGG AAAGCTATGG CAACATATTT CTGAGCAATG AGAATGGACT CCTTGGCTCC 
AAGTACAAGA AAGCAGTTTA CAGGGAGTAC ACTGATGGGA CATTCAAGAT ACTCAAGAAC 
AGGAGCAGTG ATGATGAGCA CTTAGGGATC TTGGGTCCTT TTATCTGGGC AGAAGTTGGA 
GATGTCCTCA ATATTGTGTT TAAGAACAGT GCTACCCGCC CCTACTCCAT TCATGCACAT 
GGGGTACTGG AAAAGAATGA TGGACAGCTG GAAGCTGCAA TGCCTGGTGA AATTGTAACA 
TACCGGTGGG ACATCCCGGA AAGGTCTGGC CCAGGATCCA ATGATTCTGC CTGTGTTTCG 
TGGGTCTACT ACTCCATGGT AGACCCAGTA AAGGATATGT ACAGTGGCCT GATTGGCCCC 
CTAAAGGTCT GCCGAAAAGG GATACTGCAC CCTAATGGGA AAAGGAAGGA CATGAACAAG 
GAGTTTGCTC TCCTTTTCCT GGTTTTTGAT GAAAATCAGT CCTGGTATCT GGAGGAAAAT 
GTGGAAACTC ATGGGGATCA GAGCAAGATC AACCTGGAAG ATGAACGATT TATAGAGAGC 
AATAAATTGC ACGCCATAAA CGGTAGGGTC TATGCAAACC TGCCTGGGCT GACTATGTAC 
CAAGGAGACT GGGTGAGCTG GTACCTACTG GGAATGGGCC AAGAGATTGA TGTGCACACA 
GTCCATTTTC ATGCCGAAAG CTTCACACAG AGGTATGGCA AGATGTACAG AGCGGATGTT 
GTGGATCTGT TTCCTGGGAC TTTTGTGATG GTGGAGATGC TGGTTGGGAA CCCTGGGACT 
TGGCTGCTTC ATTGCCATGT TTCAGATCAT ATCCACGCTG GTATGGAAAT ACTTTTCCAC 
GTCTTACACA AACCAGAGCC AGTGGCAAAA GTCAAACAGG CTGGAGGAGT GACAACTCCT 
CCGGATGATG AGTCAAAGTA TTTCAGTATG TTTGGATATA AACTGGATCC AGGGCAAGTG 
CAGACCACAA TCATCATTCT GACCGTTGCA GGAATTGTAC TGTTCCTCAC TGCCAGCTTC 
CTGCTGGGAG TGATCATCCG TCTTGAAAGG CAAAAAAAAT TAAGACGCAA CAGGAGATCC 
ATACTGGATG ATGGCTTCAA ACTCATGTCT CAAAAGAATC AAGTGATTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019343418.1
CDS	145..3615
Translation	MGSWWQLLQSLLYIHVLLPLFAEGVTRVYYLGIHEVKWNYAPTGKNIISDLNITADLEASAFLQPGKDRVGSIYKKSVYKQYSDSTYTTEISKPAWLGFLGPIIRAEVSDTILVHLKNFASRPYTIHPHGVFYEKDSEGSQYPDVSSPEHKKDDSIPPGGNHTYTWTIPEGHSPTADDTNCLTWIYHSHIDAPKDVASGLIGPLLTCKEGVLTGTSQRRHDMDLDFFLMFSVIDENLSWYLDENIASFCTDPGSVNKEDEEFQASNRMHAINGYLFGNLPDLTMCAGDQVAWHLFGMGNEIDIHTAFFHGQMLTIRDHKTDVASLFPATFVTAHMIAINPGKWLLSCPVNDHFKAGMEALYEVRHCSQKTPESALTGKVRTYYIAAKEEQWNYGPSGLDLSTEESLQEPGSSAEQFFKRGPGRIGGTYWKARYTEYTDATFRVEKERLKEEKHLGILGPVIKAEVGDTILVMFANKASWPFSIQPHGVLYGKEWEGAIYHNGLSWNGVSVKPLHNFTYRWTVPQHVGPTASDPPCLTWMYSSAVNPYRDTNSGLVGPLLICKPGTLSENNKQKGIDKEFYLLFSVFDENLSWYLSANIKYHLKVEEASVEKNEAFEESNRMHAINGFMFSNMPKLEVCKGDTVAWHLMGLGTEADVHGAVFQGNTFQINGMRRDTVNLFPHTFATAFMQPDNKGNFEIYCQTTNHYLSGMREQYFVSECGKKIPALAQRYEGVRTYYITAEEVEWDYAPDRSWELKRYNNSGKESYGNIFLSNENGLLGSKYKKAVYREYTDGTFKILKNRSSDDEHLGILGPFIWAEVGDVLNIVFKNSATRPYSIHAHGVLEKNDGQLEAAMPGEIVTYRWDIPERSGPGSNDSACVSWVYYSMVDPVKDMYSGLIGPLKVCRKGILHPNGKRKDMNKEFALLFLVFDENQSWYLEENVETHGDQSKINLEDERFIESNKLHAINGRVYANLPGLTMYQGDWVSWYLLGMGQEIDVHTVHFHAESFTQRYGKMYRADVVDLFPGTFVMVEMLVGNPGTWLLHCHVSDHIHAGMEILFHVLHKPEPVAKVKQAGGVTTPPDDESKYFSMFGYKLDPGQVQTTIIILTVAGIVLFLTASFLLGVIIRLERQKKLRRNRRSILDDGFKLMSQKNQVI

Target ORF information:

RefSeq Version	XM_019487873.1
Organism	Alligator mississippiensis(American alligator)
Definition	Alligator mississippiensis hephaestin (HEPH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019487873.1

ORF Insert Sequence:

ATGGGTTCCT GGTGGCAACT GCTACAGTCC TTGCTTTACA TCCATGTGCT GCTGCCACTC 
TTTGCTGAAG GTGTCACCAG AGTCTATTAC CTGGGAATCC ACGAGGTGAA ATGGAACTAT 
GCTCCAACAG GGAAGAACAT AATCAGCGAC CTAAACATTA CAGCTGACTT GGAAGCCTCT 
GCATTCCTCC AGCCTGGCAA GGACAGGGTA GGAAGCATAT ACAAAAAATC TGTCTATAAG 
CAATACTCAG ACTCCACGTA CACAACTGAG ATCAGCAAAC CTGCCTGGCT GGGGTTTCTT 
GGGCCCATCA TCCGAGCTGA AGTGAGCGAT ACCATTCTTG TGCACCTAAA AAATTTTGCC 
AGTCGGCCAT ACACGATCCA TCCTCATGGC GTGTTCTATG AAAAGGACTC AGAAGGGTCC 
CAGTACCCCG ATGTGTCCTC ACCGGAGCAT AAGAAGGATG ATTCCATTCC ACCAGGAGGG 
AATCATACAT ATACTTGGAC TATACCTGAA GGTCATAGCC CAACAGCTGA TGACACAAAC 
TGCTTGACCT GGATCTACCA CTCTCATATT GATGCACCAA AGGATGTTGC TTCTGGGTTA 
ATTGGGCCAT TACTCACATG CAAAGAAGGA GTGCTAACTG GCACCTCCCA AAGGAGGCAT 
GACATGGACC TTGATTTCTT TTTGATGTTC AGTGTCATAG ATGAGAACCT GAGCTGGTAC 
TTGGATGAGA ATATTGCTTC TTTCTGCACT GACCCAGGCT CTGTCAACAA GGAAGATGAG 
GAGTTTCAAG CGAGCAACAG AATGCATGCT ATTAATGGTT ACTTGTTTGG CAACCTGCCA 
GATCTGACAA TGTGTGCTGG GGATCAGGTA GCATGGCACC TCTTTGGGAT GGGCAATGAG 
ATTGACATCC ACACAGCCTT CTTCCATGGA CAGATGCTCA CCATCAGAGA CCACAAGACT 
GATGTGGCCA GCCTTTTCCC AGCTACTTTT GTGACAGCCC ACATGATTGC CATTAACCCT 
GGGAAATGGC TTCTGAGCTG TCCGGTCAAT GATCACTTCA AAGCTGGAAT GGAGGCACTC 
TATGAAGTCA GACATTGTTC TCAGAAAACT CCGGAGTCAG CCCTTACAGG GAAAGTCCGG 
ACATACTACA TAGCTGCAAA GGAAGAACAG TGGAATTATG GACCCTCAGG ACTTGACCTG 
AGCACTGAGG AGAGCTTGCA GGAGCCAGGG AGCTCTGCTG AGCAGTTCTT CAAGCGGGGC 
CCCGGTCGGA TTGGGGGAAC TTACTGGAAG GCCAGATACA CGGAATACAC TGATGCAACT 
TTCCGGGTGG AAAAAGAGCG ATTGAAAGAA GAGAAGCATC TTGGGATACT TGGTCCAGTG 
ATAAAAGCTG AAGTGGGAGA CACCATCTTG GTGATGTTTG CTAACAAAGC CTCCTGGCCC 
TTCAGCATTC AGCCCCATGG AGTACTTTAT GGGAAGGAAT GGGAAGGAGC TATCTATCAC 
AATGGCCTGT CATGGAATGG AGTCTCTGTT AAACCTCTCC ATAATTTCAC GTATCGCTGG 
ACAGTACCAC AGCATGTTGG ACCCACAGCC AGTGACCCTC CCTGTTTAAC CTGGATGTAC 
TCCTCTGCTG TGAATCCTTA CAGAGACACT AACTCAGGCT TAGTTGGCCC CCTATTGATC 
TGCAAACCTG GAACTCTGAG TGAGAACAAC AAACAGAAAG GGATCGACAA GGAGTTTTAC 
CTTCTCTTCA GTGTGTTTGA TGAGAATCTC AGCTGGTATT TAAGTGCAAA CATAAAGTAC 
CATTTGAAAG TGGAAGAGGC CTCTGTAGAA AAGAATGAAG CTTTCGAGGA GTCCAACAGA 
ATGCATGCCA TTAATGGATT TATGTTCTCT AACATGCCCA AGCTGGAGGT ATGTAAAGGA 
GACACTGTAG CCTGGCACCT GATGGGCCTG GGAACTGAGG CAGATGTGCA TGGAGCTGTG 
TTCCAGGGAA ATACCTTTCA AATTAATGGG ATGAGAAGAG ATACAGTCAA TCTCTTCCCT 
CACACATTTG CCACTGCCTT CATGCAGCCT GATAACAAAG GGAATTTTGA GATCTATTGC 
CAAACAACCA ACCACTATCT GTCTGGGATG AGGGAGCAGT ACTTTGTTTC TGAATGCGGC 
AAAAAGATTC CTGCTCTTGC CCAGCGCTAT GAAGGAGTGA GGACATACTA CATCACGGCA 
GAGGAGGTGG AGTGGGATTA TGCACCAGAT CGTAGCTGGG AGCTGAAACG ATACAACAAT 
TCTGGGAAGG AAAGCTATGG CAACATATTT CTGAGCAATG AGAATGGACT CCTTGGCTCC 
AAGTACAAGA AAGCAGTTTA CAGGGAGTAC ACTGATGGGA CATTCAAGAT ACTCAAGAAC 
AGGAGCAGTG ATGATGAGCA CTTAGGGATC TTGGGTCCTT TTATCTGGGC AGAAGTTGGA 
GATGTCCTCA ATATTGTGTT TAAGAACAGT GCTACCCGCC CCTACTCCAT TCATGCACAT 
GGGGTACTGG AAAAGAATGA TGGACAGCTG GAAGCTGCAA TGCCTGGTGA AATTGTAACA 
TACCGGTGGG ACATCCCGGA AAGGTCTGGC CCAGGATCCA ATGATTCTGC CTGTGTTTCG 
TGGGTCTACT ACTCCATGGT AGACCCAGTA AAGGATATGT ACAGTGGCCT GATTGGCCCC 
CTAAAGGTCT GCCGAAAAGG GATACTGCAC CCTAATGGGA AAAGGAAGGA CATGAACAAG 
GAGTTTGCTC TCCTTTTCCT GGTTTTTGAT GAAAATCAGT CCTGGTATCT GGAGGAAAAT 
GTGGAAACTC ATGGGGATCA GAGCAAGATC AACCTGGAAG ATGAACGATT TATAGAGAGC 
AATAAATTGC ACGCCATAAA CGGTAGGGTC TATGCAAACC TGCCTGGGCT GACTATGTAC 
CAAGGAGACT GGGTGAGCTG GTACCTACTG GGAATGGGCC AAGAGATTGA TGTGCACACA 
GTCCATTTTC ATGCCGAAAG CTTCACACAG AGGTATGGCA AGATGTACAG AGCGGATGTT 
GTGGATCTGT TTCCTGGGAC TTTTGTGATG GTGGAGATGC TGGTTGGGAA CCCTGGGACT 
TGGCTGCTTC ATTGCCATGT TTCAGATCAT ATCCACGCTG GTATGGAAAT ACTTTTCCAC 
GTCTTACACA AACCAGAGCC AGTGGCAAAA GTCAAACAGG CTGGAGGAGT GACAACTCCT 
CCGGATGATG AGTCAAAGTA TTTCAGTATG TTTGGATATA AACTGGATCC AGGGCAAGTG 
CAGACCACAA TCATCATTCT GACCGTTGCA GGAATTGTAC TGTTCCTCAC TGCCAGCTTC 
CTGCTGGGAG TGATCATCCG TCTTGAAAGG CAAAAAAAAT TAAGACGCAA CAGGAGATCC 
ATACTGGATG ATGGCTTCAA ACTCATGTCT CAAAAGAATC AAGTGATTTA G