def6 cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis)

Gene
Clones
gRNAs

Summary

Gene Symbol	def6
Entrez Gene ID	110957490
Full Name	DEF6, guanine nucleotide exchange factor
Gene Type	protein-coding
Organism	Acanthochromis polyacanthus(spiny chromis)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022203530.1	XP_022059222.1	differentially expressed in FDCP 6 homolog

Pathways from BioSystems
Gene Ontology

The following def6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the def6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc199616	XM_022203530.1 Latest version!	Acanthochromis polyacanthus DEF6, guanine nucleotide exchange factor (def6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

def6 ( XM_022203530.1 ) cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis) -> XP_022059222.1 Acanthochromis polyacanthus DEF6, guanine nucleotide exchange factor (def6), mRNA.

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CloneID	OAc199616
Clone ID Related Accession (Same CDS sequence)	XM_022203530.1
Accession Version	XM_022203530.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-06
Organism	Acanthochromis polyacanthus(spiny chromis)
Product	differentially expressed in FDCP 6 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019029654.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthochromis polyacanthus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGC GGTCTGAGCT GCTCAAGTCC ATCTGGTACG GTTTCACGGC CCTGGATCTG 
GAGAAAAGTG GGAAAGTGTC CAAGTCTCAG CTGAAGGTAT TATCTCACAA CCTGTGCACA 
GTTTTGTGTA TCCCACATGA CCCGGTGGCT TTGGAGGAGC ACTTCAGGGA TGATGACGAT 
GGTCCGGTTT CCAGTCAGGG TTACATGCCT TACCTCAACA AATACATCCT GGATAAGGTC 
GTGGAAGGTT CTTTTACTAA GGAAAACGTA GATGAGCTCT GTTGGACTCT CACAGCAAAG 
AAAAACTACC AGCCGGACAG AACCAGCAGC ACGATTCTGC CGGAGAGAGA CGCTTTTCGA 
CTGTGGTGCC TTTTTAATTT TCTCTCTGAA GACAAGTACC CTTTAGTGAT GGTTCCTGAT 
GAGGTGGAGT ATCTCCTCAA GAAGATCTGC ATGGCCATGA GCATCGAGTT CAACTGTGTG 
GAATTAGAGG ACTTCTTCTC CCAAGATTCA GTGCAGCAGA CCGGCATTAC CGTTTGGGTT 
TTTCTGGAGA TGATGAACTC TGGAAAAGTA ACCAGAGGAA TAGACAAGAG CATTATCAGC 
ATGGCTATAG AGGAAGTTTA CAGGGAGATA GTTGGTGACG TCCTTAAGGA GGGTTATCTG 
TGGAAAAAAG GTCAGCTGAG GAGAAACTGG AAGGAGCGCT GGTTCACGTT AAGGCCGAGC 
AACTTGGCTT ACTACACCGG GGAGGACCGC AAGGACTGCC AGGGAAACAT AGTCCTGGAT 
GGGAACTGCT GCGTGGAGGT GCTGCCCGAC CGAGATGGGA AGAGGTGCAT GTTTTGTCTT 
AAAACCCTTT CCAAGACTTA TGAAATGAGT GCATCAGACA CCAAACAGAG GCAGGAGTGG 
ACTACAGCCA TCCAAACGGC CATCAGGCTC CATGTCGAGG GGAAAAAGTC CCTTCACAAA 
GACCTGAAGC TGAAGCGTCG GGAGCAGCGA GAGCAGCGGG AGAAAAGACG GCAGGCGAAG 
GAGGAGGAGC TGCAGAGGCT TCGGGCTTTG CAGGAGGAAC GGGAGCGTAA ACTGGCCGAG 
CTGGAGCTCC TGAAGGAGGC GCAGAAGCAG GCTCAGACCC TCCTGGAGCA GGACGAGCAG 
AGGAGGCGCC AGCAGCACGA GCAGCTCCAG AGGGCCCTGG AGATCCAGCT TCGGGAGGCT 
GAGGAGGCCC GAGTCAGCAT GCAGGCGGAG ATGGCTCTGA AAGAAGAAGA GGCAGAGCGG 
CAGAGGAAGA GGATCCAGGA GCTGGAGGAG ATGCAGAAGC GTCTGGAGGA GGCGCTGCAG 
CAGGAGATTA AAGCCAGGCT GGATGAGGAG GCCTTCCGTT ACGCTCAAGC AGGGTTGCTG 
GCCGAGGAGG AGGAGAAGAT GAAGGCGCTG ATGGCCCTGC AGGAGGAGCA GGAGGAGTAC 
ATCCTGAAGA CGCAGAGGGA GAAGCAGGAG CTGAAGCAGG AGATGGAGGC CAAGTCTCGG 
TCCCTGGAGG AGGCGCAGAG GCAGCTGGAG GAGGTCCGCG CCAACCGGCA CAGGGTTGAC 
CAGGATGTAG TGGCCGCTCA GAGGAAACTT CGCCAGGCGA GCACAAACGT CAAACACTGG 
AACGTCCAGA TGAACAGACT GATGCGACCA ATAGGACCGG GCGAGAAGAG ACCGTCGCTG 
GGAAGCTCTT TCTCGGCCTT CCAGATCCCG ACACAGAGAG ACCCGGGGCT GCGCCTCAGA 
AGGAGATCGG GTTCTGAGGA TCAGGACGAG GAGAGCAAAG AAAACGTCGA CAGAAGAGCC 
GGGTGCGATC ACGAGAAACG TCACTCTCAT GCCTCTAACG GAGACATGGA CATCCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022059222.1
CDS	105..1964
Translation	MDLRSELLKSIWYGFTALDLEKSGKVSKSQLKVLSHNLCTVLCIPHDPVALEEHFRDDDDGPVSSQGYMPYLNKYILDKVVEGSFTKENVDELCWTLTAKKNYQPDRTSSTILPERDAFRLWCLFNFLSEDKYPLVMVPDEVEYLLKKICMAMSIEFNCVELEDFFSQDSVQQTGITVWVFLEMMNSGKVTRGIDKSIISMAIEEVYREIVGDVLKEGYLWKKGQLRRNWKERWFTLRPSNLAYYTGEDRKDCQGNIVLDGNCCVEVLPDRDGKRCMFCLKTLSKTYEMSASDTKQRQEWTTAIQTAIRLHVEGKKSLHKDLKLKRREQREQREKRRQAKEEELQRLRALQEERERKLAELELLKEAQKQAQTLLEQDEQRRRQQHEQLQRALEIQLREAEEARVSMQAEMALKEEEAERQRKRIQELEEMQKRLEEALQQEIKARLDEEAFRYAQAGLLAEEEEKMKALMALQEEQEEYILKTQREKQELKQEMEAKSRSLEEAQRQLEEVRANRHRVDQDVVAAQRKLRQASTNVKHWNVQMNRLMRPIGPGEKRPSLGSSFSAFQIPTQRDPGLRLRRRSGSEDQDEESKENVDRRAGCDHEKRHSHASNGDMDIP

Target ORF information:

RefSeq Version	XM_022203530.1
Organism	Acanthochromis polyacanthus(spiny chromis)
Definition	Acanthochromis polyacanthus DEF6, guanine nucleotide exchange factor (def6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022203530.1

ORF Insert Sequence:

ATGGACCTGC GGTCTGAGCT GCTCAAGTCC ATCTGGTACG GTTTCACGGC CCTGGATCTG 
GAGAAAAGTG GGAAAGTGTC CAAGTCTCAG CTGAAGGTAT TATCTCACAA CCTGTGCACA 
GTTTTGTGTA TCCCACATGA CCCGGTGGCT TTGGAGGAGC ACTTCAGGGA TGATGACGAT 
GGTCCGGTTT CCAGTCAGGG TTACATGCCT TACCTCAACA AATACATCCT GGATAAGGTC 
GTGGAAGGTT CTTTTACTAA GGAAAACGTA GATGAGCTCT GTTGGACTCT CACAGCAAAG 
AAAAACTACC AGCCGGACAG AACCAGCAGC ACGATTCTGC CGGAGAGAGA CGCTTTTCGA 
CTGTGGTGCC TTTTTAATTT TCTCTCTGAA GACAAGTACC CTTTAGTGAT GGTTCCTGAT 
GAGGTGGAGT ATCTCCTCAA GAAGATCTGC ATGGCCATGA GCATCGAGTT CAACTGTGTG 
GAATTAGAGG ACTTCTTCTC CCAAGATTCA GTGCAGCAGA CCGGCATTAC CGTTTGGGTT 
TTTCTGGAGA TGATGAACTC TGGAAAAGTA ACCAGAGGAA TAGACAAGAG CATTATCAGC 
ATGGCTATAG AGGAAGTTTA CAGGGAGATA GTTGGTGACG TCCTTAAGGA GGGTTATCTG 
TGGAAAAAAG GTCAGCTGAG GAGAAACTGG AAGGAGCGCT GGTTCACGTT AAGGCCGAGC 
AACTTGGCTT ACTACACCGG GGAGGACCGC AAGGACTGCC AGGGAAACAT AGTCCTGGAT 
GGGAACTGCT GCGTGGAGGT GCTGCCCGAC CGAGATGGGA AGAGGTGCAT GTTTTGTCTT 
AAAACCCTTT CCAAGACTTA TGAAATGAGT GCATCAGACA CCAAACAGAG GCAGGAGTGG 
ACTACAGCCA TCCAAACGGC CATCAGGCTC CATGTCGAGG GGAAAAAGTC CCTTCACAAA 
GACCTGAAGC TGAAGCGTCG GGAGCAGCGA GAGCAGCGGG AGAAAAGACG GCAGGCGAAG 
GAGGAGGAGC TGCAGAGGCT TCGGGCTTTG CAGGAGGAAC GGGAGCGTAA ACTGGCCGAG 
CTGGAGCTCC TGAAGGAGGC GCAGAAGCAG GCTCAGACCC TCCTGGAGCA GGACGAGCAG 
AGGAGGCGCC AGCAGCACGA GCAGCTCCAG AGGGCCCTGG AGATCCAGCT TCGGGAGGCT 
GAGGAGGCCC GAGTCAGCAT GCAGGCGGAG ATGGCTCTGA AAGAAGAAGA GGCAGAGCGG 
CAGAGGAAGA GGATCCAGGA GCTGGAGGAG ATGCAGAAGC GTCTGGAGGA GGCGCTGCAG 
CAGGAGATTA AAGCCAGGCT GGATGAGGAG GCCTTCCGTT ACGCTCAAGC AGGGTTGCTG 
GCCGAGGAGG AGGAGAAGAT GAAGGCGCTG ATGGCCCTGC AGGAGGAGCA GGAGGAGTAC 
ATCCTGAAGA CGCAGAGGGA GAAGCAGGAG CTGAAGCAGG AGATGGAGGC CAAGTCTCGG 
TCCCTGGAGG AGGCGCAGAG GCAGCTGGAG GAGGTCCGCG CCAACCGGCA CAGGGTTGAC 
CAGGATGTAG TGGCCGCTCA GAGGAAACTT CGCCAGGCGA GCACAAACGT CAAACACTGG 
AACGTCCAGA TGAACAGACT GATGCGACCA ATAGGACCGG GCGAGAAGAG ACCGTCGCTG 
GGAAGCTCTT TCTCGGCCTT CCAGATCCCG ACACAGAGAG ACCCGGGGCT GCGCCTCAGA 
AGGAGATCGG GTTCTGAGGA TCAGGACGAG GAGAGCAAAG AAAACGTCGA CAGAAGAGCC 
GGGTGCGATC ACGAGAAACG TCACTCTCAT GCCTCTAACG GAGACATGGA CATCCCTTAA