swt1 cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis)

Gene
Clones
gRNAs

Summary

Gene Symbol	swt1
Entrez Gene ID	110954989
Full Name	SWT1, RNA endoribonuclease homolog
Gene Type	protein-coding
Organism	Acanthochromis polyacanthus(spiny chromis)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022199760.1	XP_022055452.1	transcriptional protein SWT1
XM_022199761.1	XP_022055453.1	transcriptional protein SWT1

Pathways from BioSystems
Gene Ontology

The following swt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the swt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc196581	XM_022199760.1 Latest version!	Acanthochromis polyacanthus SWT1, RNA endoribonuclease homolog (swt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAc196581	XM_022199761.1 Latest version!	Acanthochromis polyacanthus SWT1, RNA endoribonuclease homolog (swt1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

swt1 ( XM_022199760.1 ) cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis) -> XP_022055452.1 Acanthochromis polyacanthus SWT1, RNA endoribonuclease homolog (swt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAc196581
Clone ID Related Accession (Same CDS sequence)	XM_022199760.1 , XM_022199761.1
Accession Version	XM_022199760.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2901bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-06
Organism	Acanthochromis polyacanthus(spiny chromis)
Product	transcriptional protein SWT1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019029498.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthochromis polyacanthus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAAA AGTCAAAAAA GAGAAGACAC AAGAAGTCTT CATCCTCAAG TGAAGAAGAT 
GAGAAGGTAT CAACGGAACA AGATGGCAAG AGAAAGAAAG CTGTAAAGAG TCACGAGAGG 
AGCCATAAGG AAAAAGAAAA ATCTTTTCCC TCTACTGTTA AAGATGACTC CCAAAGCAGC 
CGCCAGATCA AAAAGCCTGT CTACAGATTG GCAAACACAC AAGCAACAGA ACAGAAACCC 
ATTAAGAAGG AAGAACAATG TACTAAAACA AAAAGTGTCT CTGCGCCATT CCGTGGGGAA 
AATTGCTCCA GCAAAGCAAA ACATGATGTT TCAGGGAAAG GTAAAACTGT CAGTGGAAGT 
AAGACTGTGG AAAGGGAACC CTCAACACAC TTGAACACTA GCAGTTTACA TACACCTCCT 
CTGAGAACAG AGAAGACGTC TAAAGACAGG TTAGATGAAA AGTCTTCCCA GAGGAGCCCA 
TCCAAATTAA GGAAGTGCTC AACAAGTCCC AGTTCAGTCT CTGCTGAACA CAAGCGGAGG 
CACGATTCAC ACAGGAGGAA GCATATAGCT AAGGAACCGT CAAGAACCAG AAATGACAGT 
AGCACTCACA AGACCTGGGA ACCTACCTCT TCTTCCTCCA AGGATTCTCT AGAGCAAAGA 
AGAAGGGAGC TGGTCAAAGA TGAGCACCAC AGGCTTCAAG AAAAGGAGTC AAAGGCCAGA 
AGGAGCTTGT GTGCAGAGGC AGAATCTTCC TCTGCCTCTG TCCCAAAGAC GTCTTCCAGC 
TTAGCCAAGC ATACTTCTTC AGAGAGTTAC TCTCAGGTGC TGAAGAAAGA CACTTCAGTT 
TCATGGACTG TCACATCAGG GTCACACAAA ACAGCTCAGT CATCATCTAC TCAAACACAA 
ATACCTCCAC TCATACCGTC GATGCCTCCT GATTTTAAGA TACCAAAAAA GGTTCAGCCA 
AGACAAGTTG ATGACAATAA AACAATTTCC ACAATCACAA ATGTCAAACA CGGAGTTAAG 
CTCTCAGACT CAGAGAAGGT TTCAGTGAGC AGCACGGAGC AGGAAACTGT CCAACAAGCT 
CACAGCTGTT TGGATGTTGC ACATAAGTTT GAAGGACAAG ATAAAAAGTC GTCTTTGCCT 
CACCAGCTCC CAGCCACCAC CACTAAGCCT TGGCATGACC AGATGCAAGT GGTTGAAGAG 
CTTCATCTTG CTCGCTCTGA GAAGAGACTG GAGGTCAGTA TCATGCAGAG CTATGGGGAG 
CTCACCTGCA TGGACGTAGA TTCTGAAGGA GAACCACCCG CAGATACACA ATGCAGACGG 
CCTCCTCAGC AAGGCCTGAT CCTCGTTCTG GACACCAACA TTCTCCTCAG TCACCTGGAT 
TATGTGAAGA AGATCAGATT TCATGGCCTT GGAGCCCTGG GCTTCCCTGT CATCCTGATC 
CCCTGGGTGG TGCTTCAGGA GTTGGATTCC CTAAAAAGAG GAAAAGGCTT TGCAGGATCT 
GTGGCCCACC TGGCAACTCC TGCTATCTCG TACATTTACA ACTCGCTGAA GAGCCGAGAA 
CCTCACCTCT GGGGGCAGTC GATGCAGCAG GCCGCTGAGA GCATCAATGG TCTGAATGCT 
GAGAATAATG ATGACAGAGT GCTGCAGTGC TGCCTGCAAT ACCAGAATCT GTATCCAGAG 
TGTGCTCTTA TCCTGTGCAC CAATGATAAG AATCTGTGCA GTAAAGGTCT CCTGAGTGGG 
GTGCATACCC TCTGCAAGAG TGATTTGGAG TCAAAGGTTC AGAGATCCAG ACATGGCCTT 
GACATGCTGG AAAACATTCA GGCTCCCATG CTGCCTCACG TCAGCCCCCA GGTCTCATCA 
TCAATGCTAA GCAGGAGCAG CACACCAGTT CAGCCACACA GTCAAGAGAA AGCAGGCTGT 
TCTGTCGGAC TCACAGAGAA GGATATCAAG CGGCTCGGTG AAAGAGACGA GGAAAAGTCA 
AAATGGGACC TTAGAAGAAA TGTTTCTGGA CTAGAGGACT GTTTACTTGA GGTGCTGTCT 
GATGTGTTAG AGGCGGAGAT GAAGGCTGCT TACGAAGACC TCTGGATAGA GGTAGTTTAT 
CAAAAGCCAC CCTGGACTCT GAAAGATGTG CTGCAGTGTT TTAAAAAGCA CTGGATTGCT 
GTTTTTGGGT TCTGTGTCCC ACGCAGTAAG CTGCAAACTG TTTTGAATCT CATCAACTTC 
TTTAACTCAG GTGAAACAGT GGACTGTAGC ACTACCTCAG TGGCAATTCA AGAAGCCAAG 
GAGCTTGTGA AAGCTTTCTC GAAAAGTTCG ACCCTTGTTC CCAATGCCAT CTTTGTAATG 
GACGACATAT TCAATAAGCT GCAATCTCAG CTGCATCCAT CTGCGGAGGG CGAGTCTTCT 
TCCTCTGATG TTGTCATGAA TGATGATGAT GAAGAAGAGC AGCCCACACC TGCTCAGGTT 
TCTCACCAGG AAGTGTGGGC TGTGTTTGAG GGCATCTGGT CTAAAGTTTA CCCAATGAGC 
TTGGAAGTGT TCAAAGCTCT GGGCTTTGAC CCTTACACCA TGCAGAGTGC TCAGCCAGTG 
GGAGGTCCCC CACCGCCACA GGACGCCGTA GCCTGTTTGC ACAAACTGTC CTCCATGGTC 
TCACAGCTGC TCCAAGCCTT CAGCAGTGTT TTGTCCTCAG CTCCTGGTTT GGAGGAAGTC 
CAGAATCTGC TCAGCATCAT CCAGTCCAAT AAGCTTACTA ATGAAGAGTC CAGACTAACA 
GCCAAAGACC TTCTTGACTG TTTCTTACAA CCAGACTACA GGGAGAAGCT CAGAGTCGGA 
GGGAGCCAGT TAATGGAGCT GCAGCAGGCC TTGGATCGTT GTGTCTGGGC CACGAGTCAA 
CACACCACCT TCCCCATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022055452.1
CDS	47..2947
Translation	MSKKSKKRRHKKSSSSSEEDEKVSTEQDGKRKKAVKSHERSHKEKEKSFPSTVKDDSQSSRQIKKPVYRLANTQATEQKPIKKEEQCTKTKSVSAPFRGENCSSKAKHDVSGKGKTVSGSKTVEREPSTHLNTSSLHTPPLRTEKTSKDRLDEKSSQRSPSKLRKCSTSPSSVSAEHKRRHDSHRRKHIAKEPSRTRNDSSTHKTWEPTSSSSKDSLEQRRRELVKDEHHRLQEKESKARRSLCAEAESSSASVPKTSSSLAKHTSSESYSQVLKKDTSVSWTVTSGSHKTAQSSSTQTQIPPLIPSMPPDFKIPKKVQPRQVDDNKTISTITNVKHGVKLSDSEKVSVSSTEQETVQQAHSCLDVAHKFEGQDKKSSLPHQLPATTTKPWHDQMQVVEELHLARSEKRLEVSIMQSYGELTCMDVDSEGEPPADTQCRRPPQQGLILVLDTNILLSHLDYVKKIRFHGLGALGFPVILIPWVVLQELDSLKRGKGFAGSVAHLATPAISYIYNSLKSREPHLWGQSMQQAAESINGLNAENNDDRVLQCCLQYQNLYPECALILCTNDKNLCSKGLLSGVHTLCKSDLESKVQRSRHGLDMLENIQAPMLPHVSPQVSSSMLSRSSTPVQPHSQEKAGCSVGLTEKDIKRLGERDEEKSKWDLRRNVSGLEDCLLEVLSDVLEAEMKAAYEDLWIEVVYQKPPWTLKDVLQCFKKHWIAVFGFCVPRSKLQTVLNLINFFNSGETVDCSTTSVAIQEAKELVKAFSKSSTLVPNAIFVMDDIFNKLQSQLHPSAEGESSSSDVVMNDDDEEEQPTPAQVSHQEVWAVFEGIWSKVYPMSLEVFKALGFDPYTMQSAQPVGGPPPPQDAVACLHKLSSMVSQLLQAFSSVLSSAPGLEEVQNLLSIIQSNKLTNEESRLTAKDLLDCFLQPDYREKLRVGGSQLMELQQALDRCVWATSQHTTFPM

Target ORF information:

RefSeq Version	XM_022199760.1
Organism	Acanthochromis polyacanthus(spiny chromis)
Definition	Acanthochromis polyacanthus SWT1, RNA endoribonuclease homolog (swt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022199760.1

ORF Insert Sequence:

ATGTCCAAAA AGTCAAAAAA GAGAAGACAC AAGAAGTCTT CATCCTCAAG TGAAGAAGAT 
GAGAAGGTAT CAACGGAACA AGATGGCAAG AGAAAGAAAG CTGTAAAGAG TCACGAGAGG 
AGCCATAAGG AAAAAGAAAA ATCTTTTCCC TCTACTGTTA AAGATGACTC CCAAAGCAGC 
CGCCAGATCA AAAAGCCTGT CTACAGATTG GCAAACACAC AAGCAACAGA ACAGAAACCC 
ATTAAGAAGG AAGAACAATG TACTAAAACA AAAAGTGTCT CTGCGCCATT CCGTGGGGAA 
AATTGCTCCA GCAAAGCAAA ACATGATGTT TCAGGGAAAG GTAAAACTGT CAGTGGAAGT 
AAGACTGTGG AAAGGGAACC CTCAACACAC TTGAACACTA GCAGTTTACA TACACCTCCT 
CTGAGAACAG AGAAGACGTC TAAAGACAGG TTAGATGAAA AGTCTTCCCA GAGGAGCCCA 
TCCAAATTAA GGAAGTGCTC AACAAGTCCC AGTTCAGTCT CTGCTGAACA CAAGCGGAGG 
CACGATTCAC ACAGGAGGAA GCATATAGCT AAGGAACCGT CAAGAACCAG AAATGACAGT 
AGCACTCACA AGACCTGGGA ACCTACCTCT TCTTCCTCCA AGGATTCTCT AGAGCAAAGA 
AGAAGGGAGC TGGTCAAAGA TGAGCACCAC AGGCTTCAAG AAAAGGAGTC AAAGGCCAGA 
AGGAGCTTGT GTGCAGAGGC AGAATCTTCC TCTGCCTCTG TCCCAAAGAC GTCTTCCAGC 
TTAGCCAAGC ATACTTCTTC AGAGAGTTAC TCTCAGGTGC TGAAGAAAGA CACTTCAGTT 
TCATGGACTG TCACATCAGG GTCACACAAA ACAGCTCAGT CATCATCTAC TCAAACACAA 
ATACCTCCAC TCATACCGTC GATGCCTCCT GATTTTAAGA TACCAAAAAA GGTTCAGCCA 
AGACAAGTTG ATGACAATAA AACAATTTCC ACAATCACAA ATGTCAAACA CGGAGTTAAG 
CTCTCAGACT CAGAGAAGGT TTCAGTGAGC AGCACGGAGC AGGAAACTGT CCAACAAGCT 
CACAGCTGTT TGGATGTTGC ACATAAGTTT GAAGGACAAG ATAAAAAGTC GTCTTTGCCT 
CACCAGCTCC CAGCCACCAC CACTAAGCCT TGGCATGACC AGATGCAAGT GGTTGAAGAG 
CTTCATCTTG CTCGCTCTGA GAAGAGACTG GAGGTCAGTA TCATGCAGAG CTATGGGGAG 
CTCACCTGCA TGGACGTAGA TTCTGAAGGA GAACCACCCG CAGATACACA ATGCAGACGG 
CCTCCTCAGC AAGGCCTGAT CCTCGTTCTG GACACCAACA TTCTCCTCAG TCACCTGGAT 
TATGTGAAGA AGATCAGATT TCATGGCCTT GGAGCCCTGG GCTTCCCTGT CATCCTGATC 
CCCTGGGTGG TGCTTCAGGA GTTGGATTCC CTAAAAAGAG GAAAAGGCTT TGCAGGATCT 
GTGGCCCACC TGGCAACTCC TGCTATCTCG TACATTTACA ACTCGCTGAA GAGCCGAGAA 
CCTCACCTCT GGGGGCAGTC GATGCAGCAG GCCGCTGAGA GCATCAATGG TCTGAATGCT 
GAGAATAATG ATGACAGAGT GCTGCAGTGC TGCCTGCAAT ACCAGAATCT GTATCCAGAG 
TGTGCTCTTA TCCTGTGCAC CAATGATAAG AATCTGTGCA GTAAAGGTCT CCTGAGTGGG 
GTGCATACCC TCTGCAAGAG TGATTTGGAG TCAAAGGTTC AGAGATCCAG ACATGGCCTT 
GACATGCTGG AAAACATTCA GGCTCCCATG CTGCCTCACG TCAGCCCCCA GGTCTCATCA 
TCAATGCTAA GCAGGAGCAG CACACCAGTT CAGCCACACA GTCAAGAGAA AGCAGGCTGT 
TCTGTCGGAC TCACAGAGAA GGATATCAAG CGGCTCGGTG AAAGAGACGA GGAAAAGTCA 
AAATGGGACC TTAGAAGAAA TGTTTCTGGA CTAGAGGACT GTTTACTTGA GGTGCTGTCT 
GATGTGTTAG AGGCGGAGAT GAAGGCTGCT TACGAAGACC TCTGGATAGA GGTAGTTTAT 
CAAAAGCCAC CCTGGACTCT GAAAGATGTG CTGCAGTGTT TTAAAAAGCA CTGGATTGCT 
GTTTTTGGGT TCTGTGTCCC ACGCAGTAAG CTGCAAACTG TTTTGAATCT CATCAACTTC 
TTTAACTCAG GTGAAACAGT GGACTGTAGC ACTACCTCAG TGGCAATTCA AGAAGCCAAG 
GAGCTTGTGA AAGCTTTCTC GAAAAGTTCG ACCCTTGTTC CCAATGCCAT CTTTGTAATG 
GACGACATAT TCAATAAGCT GCAATCTCAG CTGCATCCAT CTGCGGAGGG CGAGTCTTCT 
TCCTCTGATG TTGTCATGAA TGATGATGAT GAAGAAGAGC AGCCCACACC TGCTCAGGTT 
TCTCACCAGG AAGTGTGGGC TGTGTTTGAG GGCATCTGGT CTAAAGTTTA CCCAATGAGC 
TTGGAAGTGT TCAAAGCTCT GGGCTTTGAC CCTTACACCA TGCAGAGTGC TCAGCCAGTG 
GGAGGTCCCC CACCGCCACA GGACGCCGTA GCCTGTTTGC ACAAACTGTC CTCCATGGTC 
TCACAGCTGC TCCAAGCCTT CAGCAGTGTT TTGTCCTCAG CTCCTGGTTT GGAGGAAGTC 
CAGAATCTGC TCAGCATCAT CCAGTCCAAT AAGCTTACTA ATGAAGAGTC CAGACTAACA 
GCCAAAGACC TTCTTGACTG TTTCTTACAA CCAGACTACA GGGAGAAGCT CAGAGTCGGA 
GGGAGCCAGT TAATGGAGCT GCAGCAGGCC TTGGATCGTT GTGTCTGGGC CACGAGTCAA 
CACACCACCT TCCCCATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

swt1 ( XM_022199761.1 ) cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis) -> XP_022055453.1 Acanthochromis polyacanthus SWT1, RNA endoribonuclease homolog (swt1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAc196581
Clone ID Related Accession (Same CDS sequence)	XM_022199760.1 , XM_022199761.1
Accession Version	XM_022199761.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2901bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-06
Organism	Acanthochromis polyacanthus(spiny chromis)
Product	transcriptional protein SWT1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019029498.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthochromis polyacanthus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAAA AGTCAAAAAA GAGAAGACAC AAGAAGTCTT CATCCTCAAG TGAAGAAGAT 
GAGAAGGTAT CAACGGAACA AGATGGCAAG AGAAAGAAAG CTGTAAAGAG TCACGAGAGG 
AGCCATAAGG AAAAAGAAAA ATCTTTTCCC TCTACTGTTA AAGATGACTC CCAAAGCAGC 
CGCCAGATCA AAAAGCCTGT CTACAGATTG GCAAACACAC AAGCAACAGA ACAGAAACCC 
ATTAAGAAGG AAGAACAATG TACTAAAACA AAAAGTGTCT CTGCGCCATT CCGTGGGGAA 
AATTGCTCCA GCAAAGCAAA ACATGATGTT TCAGGGAAAG GTAAAACTGT CAGTGGAAGT 
AAGACTGTGG AAAGGGAACC CTCAACACAC TTGAACACTA GCAGTTTACA TACACCTCCT 
CTGAGAACAG AGAAGACGTC TAAAGACAGG TTAGATGAAA AGTCTTCCCA GAGGAGCCCA 
TCCAAATTAA GGAAGTGCTC AACAAGTCCC AGTTCAGTCT CTGCTGAACA CAAGCGGAGG 
CACGATTCAC ACAGGAGGAA GCATATAGCT AAGGAACCGT CAAGAACCAG AAATGACAGT 
AGCACTCACA AGACCTGGGA ACCTACCTCT TCTTCCTCCA AGGATTCTCT AGAGCAAAGA 
AGAAGGGAGC TGGTCAAAGA TGAGCACCAC AGGCTTCAAG AAAAGGAGTC AAAGGCCAGA 
AGGAGCTTGT GTGCAGAGGC AGAATCTTCC TCTGCCTCTG TCCCAAAGAC GTCTTCCAGC 
TTAGCCAAGC ATACTTCTTC AGAGAGTTAC TCTCAGGTGC TGAAGAAAGA CACTTCAGTT 
TCATGGACTG TCACATCAGG GTCACACAAA ACAGCTCAGT CATCATCTAC TCAAACACAA 
ATACCTCCAC TCATACCGTC GATGCCTCCT GATTTTAAGA TACCAAAAAA GGTTCAGCCA 
AGACAAGTTG ATGACAATAA AACAATTTCC ACAATCACAA ATGTCAAACA CGGAGTTAAG 
CTCTCAGACT CAGAGAAGGT TTCAGTGAGC AGCACGGAGC AGGAAACTGT CCAACAAGCT 
CACAGCTGTT TGGATGTTGC ACATAAGTTT GAAGGACAAG ATAAAAAGTC GTCTTTGCCT 
CACCAGCTCC CAGCCACCAC CACTAAGCCT TGGCATGACC AGATGCAAGT GGTTGAAGAG 
CTTCATCTTG CTCGCTCTGA GAAGAGACTG GAGGTCAGTA TCATGCAGAG CTATGGGGAG 
CTCACCTGCA TGGACGTAGA TTCTGAAGGA GAACCACCCG CAGATACACA ATGCAGACGG 
CCTCCTCAGC AAGGCCTGAT CCTCGTTCTG GACACCAACA TTCTCCTCAG TCACCTGGAT 
TATGTGAAGA AGATCAGATT TCATGGCCTT GGAGCCCTGG GCTTCCCTGT CATCCTGATC 
CCCTGGGTGG TGCTTCAGGA GTTGGATTCC CTAAAAAGAG GAAAAGGCTT TGCAGGATCT 
GTGGCCCACC TGGCAACTCC TGCTATCTCG TACATTTACA ACTCGCTGAA GAGCCGAGAA 
CCTCACCTCT GGGGGCAGTC GATGCAGCAG GCCGCTGAGA GCATCAATGG TCTGAATGCT 
GAGAATAATG ATGACAGAGT GCTGCAGTGC TGCCTGCAAT ACCAGAATCT GTATCCAGAG 
TGTGCTCTTA TCCTGTGCAC CAATGATAAG AATCTGTGCA GTAAAGGTCT CCTGAGTGGG 
GTGCATACCC TCTGCAAGAG TGATTTGGAG TCAAAGGTTC AGAGATCCAG ACATGGCCTT 
GACATGCTGG AAAACATTCA GGCTCCCATG CTGCCTCACG TCAGCCCCCA GGTCTCATCA 
TCAATGCTAA GCAGGAGCAG CACACCAGTT CAGCCACACA GTCAAGAGAA AGCAGGCTGT 
TCTGTCGGAC TCACAGAGAA GGATATCAAG CGGCTCGGTG AAAGAGACGA GGAAAAGTCA 
AAATGGGACC TTAGAAGAAA TGTTTCTGGA CTAGAGGACT GTTTACTTGA GGTGCTGTCT 
GATGTGTTAG AGGCGGAGAT GAAGGCTGCT TACGAAGACC TCTGGATAGA GGTAGTTTAT 
CAAAAGCCAC CCTGGACTCT GAAAGATGTG CTGCAGTGTT TTAAAAAGCA CTGGATTGCT 
GTTTTTGGGT TCTGTGTCCC ACGCAGTAAG CTGCAAACTG TTTTGAATCT CATCAACTTC 
TTTAACTCAG GTGAAACAGT GGACTGTAGC ACTACCTCAG TGGCAATTCA AGAAGCCAAG 
GAGCTTGTGA AAGCTTTCTC GAAAAGTTCG ACCCTTGTTC CCAATGCCAT CTTTGTAATG 
GACGACATAT TCAATAAGCT GCAATCTCAG CTGCATCCAT CTGCGGAGGG CGAGTCTTCT 
TCCTCTGATG TTGTCATGAA TGATGATGAT GAAGAAGAGC AGCCCACACC TGCTCAGGTT 
TCTCACCAGG AAGTGTGGGC TGTGTTTGAG GGCATCTGGT CTAAAGTTTA CCCAATGAGC 
TTGGAAGTGT TCAAAGCTCT GGGCTTTGAC CCTTACACCA TGCAGAGTGC TCAGCCAGTG 
GGAGGTCCCC CACCGCCACA GGACGCCGTA GCCTGTTTGC ACAAACTGTC CTCCATGGTC 
TCACAGCTGC TCCAAGCCTT CAGCAGTGTT TTGTCCTCAG CTCCTGGTTT GGAGGAAGTC 
CAGAATCTGC TCAGCATCAT CCAGTCCAAT AAGCTTACTA ATGAAGAGTC CAGACTAACA 
GCCAAAGACC TTCTTGACTG TTTCTTACAA CCAGACTACA GGGAGAAGCT CAGAGTCGGA 
GGGAGCCAGT TAATGGAGCT GCAGCAGGCC TTGGATCGTT GTGTCTGGGC CACGAGTCAA 
CACACCACCT TCCCCATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022055453.1
CDS	131..3031
Translation	MSKKSKKRRHKKSSSSSEEDEKVSTEQDGKRKKAVKSHERSHKEKEKSFPSTVKDDSQSSRQIKKPVYRLANTQATEQKPIKKEEQCTKTKSVSAPFRGENCSSKAKHDVSGKGKTVSGSKTVEREPSTHLNTSSLHTPPLRTEKTSKDRLDEKSSQRSPSKLRKCSTSPSSVSAEHKRRHDSHRRKHIAKEPSRTRNDSSTHKTWEPTSSSSKDSLEQRRRELVKDEHHRLQEKESKARRSLCAEAESSSASVPKTSSSLAKHTSSESYSQVLKKDTSVSWTVTSGSHKTAQSSSTQTQIPPLIPSMPPDFKIPKKVQPRQVDDNKTISTITNVKHGVKLSDSEKVSVSSTEQETVQQAHSCLDVAHKFEGQDKKSSLPHQLPATTTKPWHDQMQVVEELHLARSEKRLEVSIMQSYGELTCMDVDSEGEPPADTQCRRPPQQGLILVLDTNILLSHLDYVKKIRFHGLGALGFPVILIPWVVLQELDSLKRGKGFAGSVAHLATPAISYIYNSLKSREPHLWGQSMQQAAESINGLNAENNDDRVLQCCLQYQNLYPECALILCTNDKNLCSKGLLSGVHTLCKSDLESKVQRSRHGLDMLENIQAPMLPHVSPQVSSSMLSRSSTPVQPHSQEKAGCSVGLTEKDIKRLGERDEEKSKWDLRRNVSGLEDCLLEVLSDVLEAEMKAAYEDLWIEVVYQKPPWTLKDVLQCFKKHWIAVFGFCVPRSKLQTVLNLINFFNSGETVDCSTTSVAIQEAKELVKAFSKSSTLVPNAIFVMDDIFNKLQSQLHPSAEGESSSSDVVMNDDDEEEQPTPAQVSHQEVWAVFEGIWSKVYPMSLEVFKALGFDPYTMQSAQPVGGPPPPQDAVACLHKLSSMVSQLLQAFSSVLSSAPGLEEVQNLLSIIQSNKLTNEESRLTAKDLLDCFLQPDYREKLRVGGSQLMELQQALDRCVWATSQHTTFPM

Target ORF information:

RefSeq Version	XM_022199761.1
Organism	Acanthochromis polyacanthus(spiny chromis)
Definition	Acanthochromis polyacanthus SWT1, RNA endoribonuclease homolog (swt1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022199761.1

ORF Insert Sequence:

ATGTCCAAAA AGTCAAAAAA GAGAAGACAC AAGAAGTCTT CATCCTCAAG TGAAGAAGAT 
GAGAAGGTAT CAACGGAACA AGATGGCAAG AGAAAGAAAG CTGTAAAGAG TCACGAGAGG 
AGCCATAAGG AAAAAGAAAA ATCTTTTCCC TCTACTGTTA AAGATGACTC CCAAAGCAGC 
CGCCAGATCA AAAAGCCTGT CTACAGATTG GCAAACACAC AAGCAACAGA ACAGAAACCC 
ATTAAGAAGG AAGAACAATG TACTAAAACA AAAAGTGTCT CTGCGCCATT CCGTGGGGAA 
AATTGCTCCA GCAAAGCAAA ACATGATGTT TCAGGGAAAG GTAAAACTGT CAGTGGAAGT 
AAGACTGTGG AAAGGGAACC CTCAACACAC TTGAACACTA GCAGTTTACA TACACCTCCT 
CTGAGAACAG AGAAGACGTC TAAAGACAGG TTAGATGAAA AGTCTTCCCA GAGGAGCCCA 
TCCAAATTAA GGAAGTGCTC AACAAGTCCC AGTTCAGTCT CTGCTGAACA CAAGCGGAGG 
CACGATTCAC ACAGGAGGAA GCATATAGCT AAGGAACCGT CAAGAACCAG AAATGACAGT 
AGCACTCACA AGACCTGGGA ACCTACCTCT TCTTCCTCCA AGGATTCTCT AGAGCAAAGA 
AGAAGGGAGC TGGTCAAAGA TGAGCACCAC AGGCTTCAAG AAAAGGAGTC AAAGGCCAGA 
AGGAGCTTGT GTGCAGAGGC AGAATCTTCC TCTGCCTCTG TCCCAAAGAC GTCTTCCAGC 
TTAGCCAAGC ATACTTCTTC AGAGAGTTAC TCTCAGGTGC TGAAGAAAGA CACTTCAGTT 
TCATGGACTG TCACATCAGG GTCACACAAA ACAGCTCAGT CATCATCTAC TCAAACACAA 
ATACCTCCAC TCATACCGTC GATGCCTCCT GATTTTAAGA TACCAAAAAA GGTTCAGCCA 
AGACAAGTTG ATGACAATAA AACAATTTCC ACAATCACAA ATGTCAAACA CGGAGTTAAG 
CTCTCAGACT CAGAGAAGGT TTCAGTGAGC AGCACGGAGC AGGAAACTGT CCAACAAGCT 
CACAGCTGTT TGGATGTTGC ACATAAGTTT GAAGGACAAG ATAAAAAGTC GTCTTTGCCT 
CACCAGCTCC CAGCCACCAC CACTAAGCCT TGGCATGACC AGATGCAAGT GGTTGAAGAG 
CTTCATCTTG CTCGCTCTGA GAAGAGACTG GAGGTCAGTA TCATGCAGAG CTATGGGGAG 
CTCACCTGCA TGGACGTAGA TTCTGAAGGA GAACCACCCG CAGATACACA ATGCAGACGG 
CCTCCTCAGC AAGGCCTGAT CCTCGTTCTG GACACCAACA TTCTCCTCAG TCACCTGGAT 
TATGTGAAGA AGATCAGATT TCATGGCCTT GGAGCCCTGG GCTTCCCTGT CATCCTGATC 
CCCTGGGTGG TGCTTCAGGA GTTGGATTCC CTAAAAAGAG GAAAAGGCTT TGCAGGATCT 
GTGGCCCACC TGGCAACTCC TGCTATCTCG TACATTTACA ACTCGCTGAA GAGCCGAGAA 
CCTCACCTCT GGGGGCAGTC GATGCAGCAG GCCGCTGAGA GCATCAATGG TCTGAATGCT 
GAGAATAATG ATGACAGAGT GCTGCAGTGC TGCCTGCAAT ACCAGAATCT GTATCCAGAG 
TGTGCTCTTA TCCTGTGCAC CAATGATAAG AATCTGTGCA GTAAAGGTCT CCTGAGTGGG 
GTGCATACCC TCTGCAAGAG TGATTTGGAG TCAAAGGTTC AGAGATCCAG ACATGGCCTT 
GACATGCTGG AAAACATTCA GGCTCCCATG CTGCCTCACG TCAGCCCCCA GGTCTCATCA 
TCAATGCTAA GCAGGAGCAG CACACCAGTT CAGCCACACA GTCAAGAGAA AGCAGGCTGT 
TCTGTCGGAC TCACAGAGAA GGATATCAAG CGGCTCGGTG AAAGAGACGA GGAAAAGTCA 
AAATGGGACC TTAGAAGAAA TGTTTCTGGA CTAGAGGACT GTTTACTTGA GGTGCTGTCT 
GATGTGTTAG AGGCGGAGAT GAAGGCTGCT TACGAAGACC TCTGGATAGA GGTAGTTTAT 
CAAAAGCCAC CCTGGACTCT GAAAGATGTG CTGCAGTGTT TTAAAAAGCA CTGGATTGCT 
GTTTTTGGGT TCTGTGTCCC ACGCAGTAAG CTGCAAACTG TTTTGAATCT CATCAACTTC 
TTTAACTCAG GTGAAACAGT GGACTGTAGC ACTACCTCAG TGGCAATTCA AGAAGCCAAG 
GAGCTTGTGA AAGCTTTCTC GAAAAGTTCG ACCCTTGTTC CCAATGCCAT CTTTGTAATG 
GACGACATAT TCAATAAGCT GCAATCTCAG CTGCATCCAT CTGCGGAGGG CGAGTCTTCT 
TCCTCTGATG TTGTCATGAA TGATGATGAT GAAGAAGAGC AGCCCACACC TGCTCAGGTT 
TCTCACCAGG AAGTGTGGGC TGTGTTTGAG GGCATCTGGT CTAAAGTTTA CCCAATGAGC 
TTGGAAGTGT TCAAAGCTCT GGGCTTTGAC CCTTACACCA TGCAGAGTGC TCAGCCAGTG 
GGAGGTCCCC CACCGCCACA GGACGCCGTA GCCTGTTTGC ACAAACTGTC CTCCATGGTC 
TCACAGCTGC TCCAAGCCTT CAGCAGTGTT TTGTCCTCAG CTCCTGGTTT GGAGGAAGTC 
CAGAATCTGC TCAGCATCAT CCAGTCCAAT AAGCTTACTA ATGAAGAGTC CAGACTAACA 
GCCAAAGACC TTCTTGACTG TTTCTTACAA CCAGACTACA GGGAGAAGCT CAGAGTCGGA 
GGGAGCCAGT TAATGGAGCT GCAGCAGGCC TTGGATCGTT GTGTCTGGGC CACGAGTCAA 
CACACCACCT TCCCCATGTA G