rnf14 cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis)

Gene
Clones
gRNAs

Summary

Gene Symbol	rnf14
Entrez Gene ID	110953474
Full Name	ring finger protein 14
Gene Type	protein-coding
Organism	Acanthochromis polyacanthus(spiny chromis)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022197497.1	XP_022053189.1	E3 ubiquitin-protein ligase RNF14

Pathways from BioSystems
Gene Ontology

The following rnf14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rnf14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc194757	XM_022197497.1 Latest version!	Acanthochromis polyacanthus ring finger protein 14 (rnf14), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rnf14 ( XM_022197497.1 ) cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis) -> XP_022053189.1 Acanthochromis polyacanthus ring finger protein 14 (rnf14), mRNA.

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CloneID	OAc194757
Clone ID Related Accession (Same CDS sequence)	XM_022197497.1
Accession Version	XM_022197497.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-06
Organism	Acanthochromis polyacanthus(spiny chromis)
Product	E3 ubiquitin-protein ligase RNF14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019029413.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthochromis polyacanthus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGAGG ACAGGGAAGC CCAGGAGGAT GAGCTGCTTG CTTTAGCAAG TATCTATGAT 
GAAGAGGAGT TTCACCGCGC GGAGTCGGCT CAGGGGGGAG AGATGCAATT CTGCTTGGAG 
CTCCCTCCTG ATTTCAAAGT AGTCACCAAT GGAGAGAAGA AAACTGAATA TAATGTCTGC 
TTCTTGCCCC CTTTGGTGCT CAACTTTGAG CTTCCTGCAG ACTATCCCTC CACGTCCCCA 
CCGATCTTCA CTCTTAGCTC TAAATGGATG ACCAGAGCAC AAATGAGCTC TCTGTGCCGA 
CGCCTGGATG AGCTATGGGA GGAAAGCCAA GGCTGTGTGA TTCTTTTCAC ATGGATCCAG 
TTCCTCAAAG AGGAACCTCT GGACTTCCTG GGCATCCATT CTCCTCTTGA AGTCATCAGG 
GGTGGCAGTA AGGCAGGTGG TGAGCGAAGG AGAGCTGACC CTGCAGCTGC AGCTCTGTCA 
CAGTGCGGGA GTCATTCTGA CAAAGCAGAA GAGAAGAATG GAGAAGTGAA GAAGGAAAAG 
TCAGAACAGC AGTTATCATC ATCTTCTCAA CTGGACCCTC GGGCCGTCAC TTTGATGGAC 
CCGCGTGCCG ACCTGCTACC TCAGCTGCTG GACTTTGACG AGGCACAGCG GCAGAGGGTG 
TTCGACAGCA AGGTGTTCTG CTGTGGAATC TGCTTCGTGG AGAAGCTGGG CGCCAGCTGT 
CTGTGCTTTA AGGAGTGCCA ACATGTTTAC TGCAATGCCT GCATGAGCGA ATACTTCCAG 
ATCCAAATAC GGGACGGCAA CGTTCAGTGC CTTAACTGCC CTGAGCCCAA ATGCACCTCT 
TTAGCCACAC CGTTGCAGGT GAAACAGCTG GTGGATGAGG AGCTGTTTGC CCGATACGAC 
CGTTTGCTGC TTCAGTCCAG TTTGGACCTC ATGGCCGATG TGGTCTACTG TCCCCGTCAG 
TCCTGTGGCA CCGCTGTAAT GGTGGAGCCA GACACAACCA TGGGCATTTG CTCGGCTTGC 
CAGTACGCTT TCTGCACGCT CTGTAAGCTG GGCTATCATG GTCTTTCCCA CTGCAAACTC 
CCTGCAGAGG AGCTGCGTAA CCTAAGAGAT GAGTACCTGT CAGCCACAAC TGAAGGGCAG 
AAGTTTATGG AGCAGCGCTT TGGAAAGAGG GTGATCCAGA AAGCGGTGGA GGAGTCCTTC 
AGCAGAGACT GGCTCAATGA GAACTGCAAA TGCTGCCCTC GCTGTGGAAC CAATATACAG 
AAAGTGGATG GCTGTAACAA GATGACCTGT ACATCTTGTA AACAGTACTT CTGCTGGCTC 
TGCCTGGGCG TCCTCAGCAA AGTCAACCCA TACGGTCACT TTAACAACCC AAATTCACCC 
TGTTATAACC AACTCTTCCA GGGTGTAGAT CTTGATGAAG AAAATGCCTT CTGGAGCGAT 
GAGGACGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022053189.1
CDS	91..1542
Translation	MSEDREAQEDELLALASIYDEEEFHRAESAQGGEMQFCLELPPDFKVVTNGEKKTEYNVCFLPPLVLNFELPADYPSTSPPIFTLSSKWMTRAQMSSLCRRLDELWEESQGCVILFTWIQFLKEEPLDFLGIHSPLEVIRGGSKAGGERRRADPAAAALSQCGSHSDKAEEKNGEVKKEKSEQQLSSSSQLDPRAVTLMDPRADLLPQLLDFDEAQRQRVFDSKVFCCGICFVEKLGASCLCFKECQHVYCNACMSEYFQIQIRDGNVQCLNCPEPKCTSLATPLQVKQLVDEELFARYDRLLLQSSLDLMADVVYCPRQSCGTAVMVEPDTTMGICSACQYAFCTLCKLGYHGLSHCKLPAEELRNLRDEYLSATTEGQKFMEQRFGKRVIQKAVEESFSRDWLNENCKCCPRCGTNIQKVDGCNKMTCTSCKQYFCWLCLGVLSKVNPYGHFNNPNSPCYNQLFQGVDLDEENAFWSDEDD

Target ORF information:

RefSeq Version	XM_022197497.1
Organism	Acanthochromis polyacanthus(spiny chromis)
Definition	Acanthochromis polyacanthus ring finger protein 14 (rnf14), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022197497.1

ORF Insert Sequence:

ATGTCTGAGG ACAGGGAAGC CCAGGAGGAT GAGCTGCTTG CTTTAGCAAG TATCTATGAT 
GAAGAGGAGT TTCACCGCGC GGAGTCGGCT CAGGGGGGAG AGATGCAATT CTGCTTGGAG 
CTCCCTCCTG ATTTCAAAGT AGTCACCAAT GGAGAGAAGA AAACTGAATA TAATGTCTGC 
TTCTTGCCCC CTTTGGTGCT CAACTTTGAG CTTCCTGCAG ACTATCCCTC CACGTCCCCA 
CCGATCTTCA CTCTTAGCTC TAAATGGATG ACCAGAGCAC AAATGAGCTC TCTGTGCCGA 
CGCCTGGATG AGCTATGGGA GGAAAGCCAA GGCTGTGTGA TTCTTTTCAC ATGGATCCAG 
TTCCTCAAAG AGGAACCTCT GGACTTCCTG GGCATCCATT CTCCTCTTGA AGTCATCAGG 
GGTGGCAGTA AGGCAGGTGG TGAGCGAAGG AGAGCTGACC CTGCAGCTGC AGCTCTGTCA 
CAGTGCGGGA GTCATTCTGA CAAAGCAGAA GAGAAGAATG GAGAAGTGAA GAAGGAAAAG 
TCAGAACAGC AGTTATCATC ATCTTCTCAA CTGGACCCTC GGGCCGTCAC TTTGATGGAC 
CCGCGTGCCG ACCTGCTACC TCAGCTGCTG GACTTTGACG AGGCACAGCG GCAGAGGGTG 
TTCGACAGCA AGGTGTTCTG CTGTGGAATC TGCTTCGTGG AGAAGCTGGG CGCCAGCTGT 
CTGTGCTTTA AGGAGTGCCA ACATGTTTAC TGCAATGCCT GCATGAGCGA ATACTTCCAG 
ATCCAAATAC GGGACGGCAA CGTTCAGTGC CTTAACTGCC CTGAGCCCAA ATGCACCTCT 
TTAGCCACAC CGTTGCAGGT GAAACAGCTG GTGGATGAGG AGCTGTTTGC CCGATACGAC 
CGTTTGCTGC TTCAGTCCAG TTTGGACCTC ATGGCCGATG TGGTCTACTG TCCCCGTCAG 
TCCTGTGGCA CCGCTGTAAT GGTGGAGCCA GACACAACCA TGGGCATTTG CTCGGCTTGC 
CAGTACGCTT TCTGCACGCT CTGTAAGCTG GGCTATCATG GTCTTTCCCA CTGCAAACTC 
CCTGCAGAGG AGCTGCGTAA CCTAAGAGAT GAGTACCTGT CAGCCACAAC TGAAGGGCAG 
AAGTTTATGG AGCAGCGCTT TGGAAAGAGG GTGATCCAGA AAGCGGTGGA GGAGTCCTTC 
AGCAGAGACT GGCTCAATGA GAACTGCAAA TGCTGCCCTC GCTGTGGAAC CAATATACAG 
AAAGTGGATG GCTGTAACAA GATGACCTGT ACATCTTGTA AACAGTACTT CTGCTGGCTC 
TGCCTGGGCG TCCTCAGCAA AGTCAACCCA TACGGTCACT TTAACAACCC AAATTCACCC 
TGTTATAACC AACTCTTCCA GGGTGTAGAT CTTGATGAAG AAAATGCCTT CTGGAGCGAT 
GAGGACGACT GA