EEF2 cDNA ORF clone, Acanthisitta chloris(rifleman)

Gene
Clones
gRNAs

Summary

Gene Symbol	EEF2
Entrez Gene ID	103804871
Full Name	eukaryotic translation elongation factor 2
Gene Type	protein-coding
Organism	Acanthisitta chloris(rifleman)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009077505.1	XP_009075753.1	elongation factor 2

Pathways from BioSystems
Gene Ontology

The following EEF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EEF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc120149	XM_009077505.1 Latest version!	Acanthisitta chloris eukaryotic translation elongation factor 2 (EEF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EEF2 ( XM_009077505.1 ) cDNA ORF clone, Acanthisitta chloris(rifleman) -> XP_009075753.1 Acanthisitta chloris eukaryotic translation elongation factor 2 (EEF2), mRNA.

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CloneID	OAc120149
Clone ID Related Accession (Same CDS sequence)	XM_009077505.1
Accession Version	XM_009077505.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-04
Organism	Acanthisitta chloris(rifleman)
Product	elongation factor 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008679873.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthisitta chloris Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAAAA AGGCCAACAT CAGGAACATG TCCGTGATCG CCCACGTGGA CCACGGCAAA 
TCCACGCTGA CGGATTCCCT GGTGTGCAAG GCTGGGATCA TCGCCTCGGC GCGCGCCGGG 
GAGACGCGTT TCACCGACAC GCGCAAGGAC GAGCAGGAGC GCTGCATCAC CATCAAATCC 
ACAGCTATTT CTCTGTTTTA TGAGCTCTCT GAGAACGATT TGGCCTTCAT CAAGCAGAGC 
AAGGATGGAT CTGGTTTCTT GATCAACCTG ATTGACTCCC CTGGGCATGT GGATTTCTCC 
TCTGAGGTCA CTGCTGCTCT CCGTGTCACC GACGGTGCCC TGGTCGTCGT GGACTGTGTC 
TCTGGTGTGT GTGTGCAGAC AGAGACTGTG CTGCGTCAGG CCATTGCTGA GAGGATCAAA 
CCTGTGCTGA TGATGAACAA AATGGACAGA GCTTTGCTGG AGCTGCAGCT GGAACCAGAG 
GAGCTCTACC AGACCTTCCA GCGCATTGTG GAGAACGTCA ACGTCATCAT CTCCACCTAT 
GGAGAGGGAG AAAGTGGCCC CATGGGAAAT ATCATGATTG ATCCAGTGCT GGGCACCGTG 
GGCTTTGGTT CTGGCCTGCA TGGTTGGGCC TTCACCCTGA AACAGTTTGC TGAGATGTAC 
GTGGCAAAGT TTGCTGCCAA GGGAGATGCC CAGCTGAACC CGTCGGAGCG TGCCAAGAAA 
GTAGAAGACA TGATGAAGAA GCTGTGGGGA GACAGGTATT TTGATCCTGC TACTGGCAAA 
TTCAGCAAAT CTGCTACTAG CCCTGATGGA AAGAAACTGC CCAGGACCTT CTGCCAGCTC 
ATCCTTGATC CCATCTTCAA GGTGTTCGAT GCCATCATGA ACTTCAAGAA AGAAGAGGCA 
GCCAAACTCA TTGAGAAACT GGACATCAAG CTGGACAGTG AAGACAAGGA CAAGGAGGGC 
AAACCCCTGC TGAAGGCCGT GATGAGGAGG TGGCTGCCTG CTGGAGATGC TCTGCTGCAG 
ATGATCACCA TTCACCTGCC TTCCCCTGTC ACAGCTCAGA AGTATCGCTG TGAGCTGCTC 
TACGAGGGTC CCCCCGACGA TGAGGCTGCC ATAGGCATCA AGAACTGTGA CCCCAAAGGG 
CCCCTGATGA TGTACATCTC TAAAATGGTG CCAACCTCTG ACAAGGGACG TTTCTACGCT 
TTCGGACGTG TCTTCTCTGG TCTCGTCTCG ACTGGCTTGA AAGTCAGAAT CATGGGACCA 
AACTACACCC CTGGCAAGAA GGAGGATCTG TACCTGAAGC CAATTCAAAG GACCATTCTC 
ATGATGGGTC GCTACGTGGA GCCCATCGAG GACGTGCCTT GTGGAAACAT TGTGGGGTTG 
GTGGGTGTCG ACCAGTTCCT CGTGAAGACT GGAACCATCA CCACCTTTGA GCACGCTCAC 
AACATGAGGG TCATGAAGTT CAGCGTCAGC CCCGTCGTGC GTGTGGCCGT GGAGGCCAAG 
AACCCTGCTG ACCTGCCCAA GCTGGTGGAG GGGCTGAAGA GACTGGCCAA GTCTGACCCC 
ATGGTGCAGT GCATCATTGA GGAGTCTGGA GAGCACATCA TCGCTGGTGC TGGGGAGCTG 
CACCTGGAGA TCTGCCTCAA GGACCTGGAG GAGGACCATG CCTGCATCCC CATTAAGAAA 
TCAGACCCGG TGGTGTCCTA CCGCGAGACG GTCAGCGAGG AGTCCAACGT GATGTGCCTT 
TCCAAGTCCC CCAACAAACA CAACAGGCTC TACATGAAGG CCAGACCCTT CCCTGACGGC 
CTGGCCGAGG ACATCGACAA GGGGGAGGTG TCAGCCCGGC AGGAGCTGAA GCAGAGGGCG 
CGTTACCTGG CCGAGAAGTA CGAGTGGGAC GTCACCGAGG CCAGGAAGAT CTGGTGCTTC 
GGTCCTGATG GCACCGGCCC CAACATCCTG ACTGACATCA CCAAGGGAGT GCAGTACCTG 
AACGAGATCA AGGACAGCGT GGTGGCAGGA TTCCAGTGGG CCACCAAGGA GGGAGTCCTG 
TGCGAGGAGA ACATGCGTGC GGTGCGTTTC GACGTCCACG ACGTGACCCT CCACGCCGAC 
GCCATCCACC GCGGCGGTGG CCAGATCATC CCCACGGCTC GGCGCTGCCT CTACGCCTGC 
GTGCTCACCG CCCAGCCACG CCTCATGGAG CCCATCTACC TGGTGGAGAT CCAGTGCCCG 
GAGCAGGTGG TTGGTGGCAT CTATGGTGTG CTGAACAGGA AGCGTGGGCA CGTGTTCGAG 
GAGACCCAAG TGGCTGGGAC ACCCATGTTT GTGGTCAAGG CCTATCTGCC TGTCAACGAG 
TCCTTTGGTT TCACAGCAGA TTTGAGGTCC AACACAGGGG GCCAAGCCTT CCCCCAGTGT 
GTGTTTGACC ACTGGCAGAT CCTGCCTGGT GATCCCTTCG ACAGTGCCAG CAGACCCTGC 
CAGGTGGTGG CCGAGACCCG CAAGCGTAAA GGGCTCAAGG AAGGAATCCC TGCCCTTGAC 
AACTTCCTGG ATAAACTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009075753.1
CDS	49..2589
Translation	MDKKANIRNMSVIAHVDHGKSTLTDSLVCKAGIIASARAGETRFTDTRKDEQERCITIKSTAISLFYELSENDLAFIKQSKDGSGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPVLMMNKMDRALLELQLEPEELYQTFQRIVENVNVIISTYGEGESGPMGNIMIDPVLGTVGFGSGLHGWAFTLKQFAEMYVAKFAAKGDAQLNPSERAKKVEDMMKKLWGDRYFDPATGKFSKSATSPDGKKLPRTFCQLILDPIFKVFDAIMNFKKEEAAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDALLQMITIHLPSPVTAQKYRCELLYEGPPDDEAAIGIKNCDPKGPLMMYISKMVPTSDKGRFYAFGRVFSGLVSTGLKVRIMGPNYTPGKKEDLYLKPIQRTILMMGRYVEPIEDVPCGNIVGLVGVDQFLVKTGTITTFEHAHNMRVMKFSVSPVVRVAVEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVSEESNVMCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKQRARYLAEKYEWDVTEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGVLCEENMRAVRFDVHDVTLHADAIHRGGGQIIPTARRCLYACVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEETQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDSASRPCQVVAETRKRKGLKEGIPALDNFLDKL

Target ORF information:

RefSeq Version	XM_009077505.1
Organism	Acanthisitta chloris(rifleman)
Definition	Acanthisitta chloris eukaryotic translation elongation factor 2 (EEF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009077505.1

ORF Insert Sequence:

ATGGACAAAA AGGCCAACAT CAGGAACATG TCCGTGATCG CCCACGTGGA CCACGGCAAA 
TCCACGCTGA CGGATTCCCT GGTGTGCAAG GCTGGGATCA TCGCCTCGGC GCGCGCCGGG 
GAGACGCGTT TCACCGACAC GCGCAAGGAC GAGCAGGAGC GCTGCATCAC CATCAAATCC 
ACAGCTATTT CTCTGTTTTA TGAGCTCTCT GAGAACGATT TGGCCTTCAT CAAGCAGAGC 
AAGGATGGAT CTGGTTTCTT GATCAACCTG ATTGACTCCC CTGGGCATGT GGATTTCTCC 
TCTGAGGTCA CTGCTGCTCT CCGTGTCACC GACGGTGCCC TGGTCGTCGT GGACTGTGTC 
TCTGGTGTGT GTGTGCAGAC AGAGACTGTG CTGCGTCAGG CCATTGCTGA GAGGATCAAA 
CCTGTGCTGA TGATGAACAA AATGGACAGA GCTTTGCTGG AGCTGCAGCT GGAACCAGAG 
GAGCTCTACC AGACCTTCCA GCGCATTGTG GAGAACGTCA ACGTCATCAT CTCCACCTAT 
GGAGAGGGAG AAAGTGGCCC CATGGGAAAT ATCATGATTG ATCCAGTGCT GGGCACCGTG 
GGCTTTGGTT CTGGCCTGCA TGGTTGGGCC TTCACCCTGA AACAGTTTGC TGAGATGTAC 
GTGGCAAAGT TTGCTGCCAA GGGAGATGCC CAGCTGAACC CGTCGGAGCG TGCCAAGAAA 
GTAGAAGACA TGATGAAGAA GCTGTGGGGA GACAGGTATT TTGATCCTGC TACTGGCAAA 
TTCAGCAAAT CTGCTACTAG CCCTGATGGA AAGAAACTGC CCAGGACCTT CTGCCAGCTC 
ATCCTTGATC CCATCTTCAA GGTGTTCGAT GCCATCATGA ACTTCAAGAA AGAAGAGGCA 
GCCAAACTCA TTGAGAAACT GGACATCAAG CTGGACAGTG AAGACAAGGA CAAGGAGGGC 
AAACCCCTGC TGAAGGCCGT GATGAGGAGG TGGCTGCCTG CTGGAGATGC TCTGCTGCAG 
ATGATCACCA TTCACCTGCC TTCCCCTGTC ACAGCTCAGA AGTATCGCTG TGAGCTGCTC 
TACGAGGGTC CCCCCGACGA TGAGGCTGCC ATAGGCATCA AGAACTGTGA CCCCAAAGGG 
CCCCTGATGA TGTACATCTC TAAAATGGTG CCAACCTCTG ACAAGGGACG TTTCTACGCT 
TTCGGACGTG TCTTCTCTGG TCTCGTCTCG ACTGGCTTGA AAGTCAGAAT CATGGGACCA 
AACTACACCC CTGGCAAGAA GGAGGATCTG TACCTGAAGC CAATTCAAAG GACCATTCTC 
ATGATGGGTC GCTACGTGGA GCCCATCGAG GACGTGCCTT GTGGAAACAT TGTGGGGTTG 
GTGGGTGTCG ACCAGTTCCT CGTGAAGACT GGAACCATCA CCACCTTTGA GCACGCTCAC 
AACATGAGGG TCATGAAGTT CAGCGTCAGC CCCGTCGTGC GTGTGGCCGT GGAGGCCAAG 
AACCCTGCTG ACCTGCCCAA GCTGGTGGAG GGGCTGAAGA GACTGGCCAA GTCTGACCCC 
ATGGTGCAGT GCATCATTGA GGAGTCTGGA GAGCACATCA TCGCTGGTGC TGGGGAGCTG 
CACCTGGAGA TCTGCCTCAA GGACCTGGAG GAGGACCATG CCTGCATCCC CATTAAGAAA 
TCAGACCCGG TGGTGTCCTA CCGCGAGACG GTCAGCGAGG AGTCCAACGT GATGTGCCTT 
TCCAAGTCCC CCAACAAACA CAACAGGCTC TACATGAAGG CCAGACCCTT CCCTGACGGC 
CTGGCCGAGG ACATCGACAA GGGGGAGGTG TCAGCCCGGC AGGAGCTGAA GCAGAGGGCG 
CGTTACCTGG CCGAGAAGTA CGAGTGGGAC GTCACCGAGG CCAGGAAGAT CTGGTGCTTC 
GGTCCTGATG GCACCGGCCC CAACATCCTG ACTGACATCA CCAAGGGAGT GCAGTACCTG 
AACGAGATCA AGGACAGCGT GGTGGCAGGA TTCCAGTGGG CCACCAAGGA GGGAGTCCTG 
TGCGAGGAGA ACATGCGTGC GGTGCGTTTC GACGTCCACG ACGTGACCCT CCACGCCGAC 
GCCATCCACC GCGGCGGTGG CCAGATCATC CCCACGGCTC GGCGCTGCCT CTACGCCTGC 
GTGCTCACCG CCCAGCCACG CCTCATGGAG CCCATCTACC TGGTGGAGAT CCAGTGCCCG 
GAGCAGGTGG TTGGTGGCAT CTATGGTGTG CTGAACAGGA AGCGTGGGCA CGTGTTCGAG 
GAGACCCAAG TGGCTGGGAC ACCCATGTTT GTGGTCAAGG CCTATCTGCC TGTCAACGAG 
TCCTTTGGTT TCACAGCAGA TTTGAGGTCC AACACAGGGG GCCAAGCCTT CCCCCAGTGT 
GTGTTTGACC ACTGGCAGAT CCTGCCTGGT GATCCCTTCG ACAGTGCCAG CAGACCCTGC 
CAGGTGGTGG CCGAGACCCG CAAGCGTAAA GGGCTCAAGG AAGGAATCCC TGCCCTTGAC 
AACTTCCTGG ATAAACTCTA A