EARS2 cDNA ORF clone, Acanthisitta chloris(rifleman)

Gene
Clones
gRNAs

Summary

Gene Symbol	EARS2
Entrez Gene ID	103803744
Full Name	glutamyl-tRNA synthetase 2, mitochondrial
Gene Type	protein-coding
Organism	Acanthisitta chloris(rifleman)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009076317.1	XP_009074565.1	probable glutamate--tRNA ligase, mitochondrial

Pathways from BioSystems
Gene Ontology

The following EARS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EARS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc119609	XM_009076317.1 Latest version!	Acanthisitta chloris glutamyl-tRNA synthetase 2, mitochondrial (EARS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EARS2 ( XM_009076317.1 ) cDNA ORF clone, Acanthisitta chloris(rifleman) -> XP_009074565.1 Acanthisitta chloris glutamyl-tRNA synthetase 2, mitochondrial (EARS2), mRNA.

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CloneID	OAc119609
Clone ID Related Accession (Same CDS sequence)	XM_009076317.1
Accession Version	XM_009076317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-04
Organism	Acanthisitta chloris(rifleman)
Product	probable glutamate--tRNA ligase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008676929.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthisitta chloris Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

ATGGTCTTTG TTGTGACGAT CTCCTCTGAT TTCCCCACGG TTACAGGGTT TCTGCACTTG 
GGTGGCCTTC GGACTGCTCT GTACAATTAC ATCTTTGCTA AAAAGCACCA AGGGACCTTC 
ATTTTGAGAG TGGAGGATAC AGACCAGAAC CGAGTGGTAC CTGGAGCTGC AGAAGGGATA 
GAAGATATGT TGAACTGGGC AGGTATTCCC CCAGACGAGA GCCCTGGCCG TGGGGGCCCC 
ATGGGGCCCT ATGTGCAGTC CCTGAGGCTG GAGCTGTACA GCCAAGTTGT GGTGGCACTG 
CTGGCCAGTG GAGCTGCCTA TCGATGCTTC TGCACCCCCC AGCGCCTGGA GCTGCTCAGG 
AAGGAGGCCC TGCGCAGCCA GCAGACCCCA CGGTATGACA ATCGGTGTCG GCACCTGACG 
CCCACAGAAG TGGCTCAGAA GCTGTCACAG GGCCTCGACT CCGTCGTGCG CTTCCGTCTG 
GAGAAGGGGG TAGAACCCTT CCAGGACCTG GTCTATGGCT GGAGCAAGCA TGAGGTGGCT 
GAGGTGGAAG GGGACCCAGT GATTCTCAAG GGCGATGGCT TCCCCACGTA CCACCTGGCA 
AACGTGGTGG ACGACCACCA CATGGGCATC ACCCACGTCC TGCGAGGAAC TGAGTGGTTG 
ACTTCAACCT CCAAGCACCT GCTGCTCTAC AAAGCCCTTG GCTGGGAGCC CCCTCAGTTT 
GGCCACCTCC CACTGCTGCT GAACAAGGAT GGGGGGAAGC TGTCCAAGAG GCAAGGGGAC 
ATCTTCCTGG AGCGCTTTGC TCGGGATGGC TTCCTGCCAG AGGCACTGCT GGACATCATC 
ACTAACTGCG GCTCAGGGTT CCCAGAGAAG CAAATGGGGA GGACTTTGAA GGAGCTGATC 
TCACAGTTTG AAGTAGGCAG AATTACCACC CATTCTGCCC TCCTGGACCT TGAAACTCTC 
CCAGAATTCA ACAGGATTCA CCTCACCCGT CACATCGAGG ACGAAGGGCT GCGCCAGAAG 
CTCGTTAGGG AGCTGCAGGG GCTGGTGGAG TGTACCTATA GGGATCAGCA GGTGGATCCA 
GAGGTTCTGG AAAAGGAATA TGTGGAGCGA GTCCTCCTGC TGAGAAAAGG TCACATAAGC 
TTCCTGAAGA ACCTGGTGTC GAGTGATTAC TCTTACCTGT GGGTCAGGCC CTCAGTGTCC 
CGAGAGCAGC TCCAAAGCAT TTCTGCAGAA GCAGATGAAA TAGGAAAACT AGTCTTAGGG 
CTCATGACAG GGCAGGCAGC TGCCTCCACA ATGGAGGAGT TGAACAAAGA CCTGAGAGGC 
CTCCAGAAGC AAACCAGAGA GACCAAATAC AGCAGCATGA TGCAGCTCCT CCGCTTGGCA 
CTCAGTGGGC TGCAGCATGG GCCAAGTGTG GCCGAGATGA TGGTGACTCT GGGACCCAAA 
GAGGTGTGTG GTCGGATACA CAGAGCTCTG TCCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009074565.1
CDS	1..1479
Translation	MVFVVTISSDFPTVTGFLHLGGLRTALYNYIFAKKHQGTFILRVEDTDQNRVVPGAAEGIEDMLNWAGIPPDESPGRGGPMGPYVQSLRLELYSQVVVALLASGAAYRCFCTPQRLELLRKEALRSQQTPRYDNRCRHLTPTEVAQKLSQGLDSVVRFRLEKGVEPFQDLVYGWSKHEVAEVEGDPVILKGDGFPTYHLANVVDDHHMGITHVLRGTEWLTSTSKHLLLYKALGWEPPQFGHLPLLLNKDGGKLSKRQGDIFLERFARDGFLPEALLDIITNCGSGFPEKQMGRTLKELISQFEVGRITTHSALLDLETLPEFNRIHLTRHIEDEGLRQKLVRELQGLVECTYRDQQVDPEVLEKEYVERVLLLRKGHISFLKNLVSSDYSYLWVRPSVSREQLQSISAEADEIGKLVLGLMTGQAAASTMEELNKDLRGLQKQTRETKYSSMMQLLRLALSGLQHGPSVAEMMVTLGPKEVCGRIHRALSS

Target ORF information:

RefSeq Version	XM_009076317.1
Organism	Acanthisitta chloris(rifleman)
Definition	Acanthisitta chloris glutamyl-tRNA synthetase 2, mitochondrial (EARS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009076317.1

ORF Insert Sequence:

ATGGTCTTTG TTGTGACGAT CTCCTCTGAT TTCCCCACGG TTACAGGGTT TCTGCACTTG 
GGTGGCCTTC GGACTGCTCT GTACAATTAC ATCTTTGCTA AAAAGCACCA AGGGACCTTC 
ATTTTGAGAG TGGAGGATAC AGACCAGAAC CGAGTGGTAC CTGGAGCTGC AGAAGGGATA 
GAAGATATGT TGAACTGGGC AGGTATTCCC CCAGACGAGA GCCCTGGCCG TGGGGGCCCC 
ATGGGGCCCT ATGTGCAGTC CCTGAGGCTG GAGCTGTACA GCCAAGTTGT GGTGGCACTG 
CTGGCCAGTG GAGCTGCCTA TCGATGCTTC TGCACCCCCC AGCGCCTGGA GCTGCTCAGG 
AAGGAGGCCC TGCGCAGCCA GCAGACCCCA CGGTATGACA ATCGGTGTCG GCACCTGACG 
CCCACAGAAG TGGCTCAGAA GCTGTCACAG GGCCTCGACT CCGTCGTGCG CTTCCGTCTG 
GAGAAGGGGG TAGAACCCTT CCAGGACCTG GTCTATGGCT GGAGCAAGCA TGAGGTGGCT 
GAGGTGGAAG GGGACCCAGT GATTCTCAAG GGCGATGGCT TCCCCACGTA CCACCTGGCA 
AACGTGGTGG ACGACCACCA CATGGGCATC ACCCACGTCC TGCGAGGAAC TGAGTGGTTG 
ACTTCAACCT CCAAGCACCT GCTGCTCTAC AAAGCCCTTG GCTGGGAGCC CCCTCAGTTT 
GGCCACCTCC CACTGCTGCT GAACAAGGAT GGGGGGAAGC TGTCCAAGAG GCAAGGGGAC 
ATCTTCCTGG AGCGCTTTGC TCGGGATGGC TTCCTGCCAG AGGCACTGCT GGACATCATC 
ACTAACTGCG GCTCAGGGTT CCCAGAGAAG CAAATGGGGA GGACTTTGAA GGAGCTGATC 
TCACAGTTTG AAGTAGGCAG AATTACCACC CATTCTGCCC TCCTGGACCT TGAAACTCTC 
CCAGAATTCA ACAGGATTCA CCTCACCCGT CACATCGAGG ACGAAGGGCT GCGCCAGAAG 
CTCGTTAGGG AGCTGCAGGG GCTGGTGGAG TGTACCTATA GGGATCAGCA GGTGGATCCA 
GAGGTTCTGG AAAAGGAATA TGTGGAGCGA GTCCTCCTGC TGAGAAAAGG TCACATAAGC 
TTCCTGAAGA ACCTGGTGTC GAGTGATTAC TCTTACCTGT GGGTCAGGCC CTCAGTGTCC 
CGAGAGCAGC TCCAAAGCAT TTCTGCAGAA GCAGATGAAA TAGGAAAACT AGTCTTAGGG 
CTCATGACAG GGCAGGCAGC TGCCTCCACA ATGGAGGAGT TGAACAAAGA CCTGAGAGGC 
CTCCAGAAGC AAACCAGAGA GACCAAATAC AGCAGCATGA TGCAGCTCCT CCGCTTGGCA 
CTCAGTGGGC TGCAGCATGG GCCAAGTGTG GCCGAGATGA TGGTGACTCT GGGACCCAAA 
GAGGTGTGTG GTCGGATACA CAGAGCTCTG TCCAGCTGA