RALGAPA1 gene cDNA ORF clone, Ovis aries(sheep)

mRNA	Protein	Name
XM_012098817.2	XP_011954207.1	ral GTPase-activating protein subunit alpha-1 isoform X9
XM_012098818.1	XP_011954208.1	ral GTPase-activating protein subunit alpha-1 isoform X1
XM_015106613.1	XP_014962099.1	ral GTPase-activating protein subunit alpha-1 isoform X25
XM_027957249.1	XP_027813050.1	ral GTPase-activating protein subunit alpha-1 isoform X2
XM_012098813.2	XP_011954203.1	ral GTPase-activating protein subunit alpha-1 isoform X6
XM_015102009.1	XP_014957495.1	ral GTPase-activating protein subunit alpha-1 isoform X12
XM_012130791.2	XP_011986181.1	ral GTPase-activating protein subunit alpha-1 isoform X16
XM_012130792.2	XP_011986182.1	ral GTPase-activating protein subunit alpha-1 isoform X17
XM_012130799.2	XP_011986189.1	ral GTPase-activating protein subunit alpha-1 isoform X22
XM_012130803.2	XP_011986193.1	ral GTPase-activating protein subunit alpha-1 isoform X26
XM_004017895.4	XP_004017944.2	ral GTPase-activating protein subunit alpha-1 isoform X6
XM_012130804.2	XP_011986194.1	ral GTPase-activating protein subunit alpha-1 isoform X27
XM_012130793.2	XP_011986183.1	ral GTPase-activating protein subunit alpha-1 isoform X18
XM_012130795.2	XP_011986185.1	ral GTPase-activating protein subunit alpha-1 isoform X19
XM_012098812.2	XP_011954202.1	ral GTPase-activating protein subunit alpha-1 isoform X5
XM_027957251.1	XP_027813052.1	ral GTPase-activating protein subunit alpha-1 isoform X3
XM_012098821.3	XP_011954211.2	ral GTPase-activating protein subunit alpha-1 isoform X5
XM_012098819.2	XP_011954209.1	ral GTPase-activating protein subunit alpha-1 isoform X10
XM_012098821.2	XP_011954211.1	ral GTPase-activating protein subunit alpha-1 isoform X13
XM_004017895.3	XP_004017944.1	ral GTPase-activating protein subunit alpha-1 isoform X14
XM_015102010.1	XP_014957496.1	ral GTPase-activating protein subunit alpha-1 isoform X15
XM_012098809.2	XP_011954199.1	ral GTPase-activating protein subunit alpha-1 isoform X2
XM_012130796.2	XP_011986186.1	ral GTPase-activating protein subunit alpha-1 isoform X20
XM_027957250.1	XP_027813051.1	ral GTPase-activating protein subunit alpha-1 isoform X3
XM_012130797.2	XP_011986187.1	ral GTPase-activating protein subunit alpha-1 isoform X20
XM_012130798.2	XP_011986188.1	ral GTPase-activating protein subunit alpha-1 isoform X21
XM_012130800.1	XP_011986190.1	ral GTPase-activating protein subunit alpha-1 isoform X1
XM_012130801.2	XP_011986191.1	ral GTPase-activating protein subunit alpha-1 isoform X23
XM_012130802.2	XP_011986192.1	ral GTPase-activating protein subunit alpha-1 isoform X24
XM_012098815.2	XP_011954205.1	ral GTPase-activating protein subunit alpha-1 isoform X7
XM_012098811.2	XP_011954201.1	ral GTPase-activating protein subunit alpha-1 isoform X4
XM_012098814.2	XP_011954204.1	ral GTPase-activating protein subunit alpha-1 isoform X6
XM_015102008.1	XP_014957494.1	ral GTPase-activating protein subunit alpha-1 isoform X8
XM_012098820.2	XP_011954210.1	ral GTPase-activating protein subunit alpha-1 isoform X11
XM_012098810.2	XP_011954200.1	ral GTPase-activating protein subunit alpha-1 isoform X3
XM_012098811.3	XP_011954201.2	ral GTPase-activating protein subunit alpha-1 isoform X1
XM_012098820.3	XP_011954210.2	ral GTPase-activating protein subunit alpha-1 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSm53951	XM_015102010.1 Latest version!	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OOv14054	XM_012130791.2 Latest version!	Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OOv14055	XM_012130792.2 Latest version!	Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OOv14056	XM_012130793.2 Latest version!	Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OOv14057	XM_012130795.2 Latest version!	Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OOv14058	XM_012130796.2 Latest version!	Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OOv14058	XM_012130797.2 Latest version!	Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OOv14059	XM_012130798.2 Latest version!	Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OOv14060	XM_012130799.2 Latest version!	Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OOv14061	XM_012130800.1 Latest version!	Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OOv14062	XM_012130801.2 Latest version!	Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OOv14063	XM_012130802.2 Latest version!	Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OOv14064	XM_015106613.1 Latest version!	Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OOv14065	XM_012130803.2 Latest version!	Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OOv14066	XM_012130804.2 Latest version!	Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OSm53950	XM_015102009.1 Latest version!	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OSm67921	XM_012098811.3 Latest version!	Ovis aries Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSm67922	XM_027957249.1 Latest version!	Ovis aries Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSm67923	XM_027957251.1 Latest version!	Ovis aries Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSm67923	XM_027957250.1 Latest version!	Ovis aries Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSm67924	XM_012098820.3 Latest version!	Ovis aries Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSm67925	XM_012098821.3 Latest version!	Ovis aries Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSm67926	XM_004017895.4 Latest version!	Ovis aries Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSm14257	XM_004017895.3 Latest version!	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OSm41096	XM_012098809.2 Latest version!	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OSm41097	XM_012098810.2 Latest version!	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OSm41098	XM_012098811.2 Latest version!	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OSm41099	XM_012098812.2 Latest version!	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OSm41100	XM_012098814.2 Latest version!	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OSm41100	XM_012098813.2 Latest version!	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OSm41101	XM_012098815.2 Latest version!	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OSm41102	XM_015102008.1 Latest version!	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OSm41103	XM_012098817.2 Latest version!	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OSm41104	XM_012098818.1 Latest version!	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OSm41105	XM_012098819.2 Latest version!	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OSm41106	XM_012098820.2 Latest version!	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OSm41107	XM_012098821.2 Latest version!	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

RALGAPA1 ( XM_015102010.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014957496.1 Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OSm53951
Clone ID Related Accession (Same CDS sequence)	XM_015102010.1
Accession Version	XM_015102010.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	ral GTPase-activating protein subunit alpha-1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTCAT GCTTAATCCC TGGATTTTCA GCACCACAGT CTGAATATGG ACCTCGAACT 
TTAGATAACC TCATTAATCC TCCACTCAAC CTACAAGAAA CTCAAGTCAC TATAGAGGAA 
ATCACTCCAC TTGTCCCCCC ACAATCAGGA GATAAAGGAC AAGAAGATCT TACAAGCTAT 
TTTCTTGAAG CACTTTTAAA ATATATAGTA ATTCAGGTAA AAAGTTTAGA GTGGAAGAAC 
AAAGAAAACC AAGAAAAAGG ATTTTCATTT CTGTTTTCAC ATTTTAAGAA ATATTATTTG 
CCTTATATTT TCCCAAACAT CTGTAAGGAG AATAGTTTAT ATCATCCTGT ACTTGATATC 
CCACAGATGA GACCAAAGCC CCATTATGTC ATGATAAAGA AGGATGCTGA AACCAATGAG 
GCAATCTATT GTACAAAGGA GCCTTTCATT AAGGCTCGTG TTATTGTCAT TCGTTGGCTG 
GTTTCATTCT GGCTGGAGCC AAAGCCGCAT ACAGGACCTC ATATTCCTGG GATGGAAGGT 
GAAGTCTTGC CAAAAAATAT TCAGAGAGCA GCTGCTAGTT TGGTATCCAG AGAAGAAAAC 
AAAAATGATA ATGCTGATAA AACAGATAGA AGTACAGAAC CTGAACAGTC TCATTCCAAC 
ACAAGCACTC TCACGGAGCG AGAACCTAGC TCTTCTAGTC TCTGTAGTAT TGATGAAGAG 
CATCTCACAG ACATTGAAAT AGTTCGCCGA GTTTTTTCTT CTAAAAGAAG TAACGTAAAC 
TTTGTCACAG AGATATTTCG TCAGGCATTT TTATTACCAA TTTGCGAAGC AGCAGCTATG 
AGAAAAGTGG TAAAAGTATA TCAAGAATGG ATCCAGCAAG AGGAAAAACC TTTGTTCATG 
CAAGAGCCTG AAGAAATTGT GATCTCTTCT TCAGACCTTC CGTGCATTGA CAATGTCACA 
GACCATGATA TTTCAATGGA AGAAGGAGAA AAAAGAGAAG AGGAAAATGG GACCAATGTT 
GCTGATCATG TTCGAAATTC CACTTGGCCT AAAAATGGAA CCTACCAAGA TATTCTTCAT 
AATGCTTCTG AAGAAGCCAT AGAACAAAAC ATACGAGCTG GTGCCCAGGC AGTTTTGCAG 
GTGTTTATTA TAAATTCTTC AAACATATTT CTTCTTGAAC CTGCAAATGA GATAAAAAGT 
CTTCTGGATG AGCACACAGA TATGTGTAAA CGAATTCTTA ACATTTATCG GTTCATGGTT 
GTGCAAGTAT CAATGGACAA AAAGACTTGG GAACAGATGC TGCTTGTGTT GCTCAGAGTC 
ACGGAGTCTG TACTGAAGAT GCCATCACAA GCTTTTCTAC AGTTCCAGGG GAAAAAAAAC 
ATGACTTTGG CAGGTCGACT TGCAGGACCA CTTTTCCAGA CTCTTATAGT TGCCTGGATT 
AAAGCAAATC TAAATGTGTA CATCTCTCGA GAACTTTGGG ATGACTTACT CTCAGTATTG 
TCATCGTTGA CCTATTGGGA AGAGTTAGCC ACTGAATGGT CGCTAACTAT GGAGACACTA 
ACTAAAGTTT TAGCTAGAAA TTTGTATAGT TTGGATCTCA GTGATTTACC ATTGGATAAG 
CTGAGTGAAC AGAAACAAAA AAAGCACAAA GGGAAAGGAG TGGGACATGA ATTTCAGAAA 
GTTTCAGTTG ACAAGTCATT TTCTAGAGGA TGGAGTCGCG ATCAGCCTGG CCAAGCCCCA 
ATGAGGCAGA GAAGTGCAAC AACCACTGGT TCCCCAGGCA CAGAAAAGGC AAGGAGTATA 
GTACGGCAAA AAACTGTTGC CATGAGAAGC CGATCCATTG GTGAATGTGC TCTGCCATCG 
GCCTATATAC GCAGTGCTAA AAGTGCTCCT GTTCTGATCC ATACTTCCAA ACCCTTCTTG 
CCTGATATTG TTCTCACTCC CCTTTCTGAT GAGCTTTCAG ATATTGATGA TGCTCAAATA 
CTTCCTCGTC CAACTAGAGT CAGACATTTT TCGCAAAGTG AAGACACTGG AAATGAAGTT 
TTTGGTGCTT TGAGTGAGGA GCAGCCATTG CCTCGAAGTA GCAGCACTTC TGACATCTTG 
GAACCATTCA CTGTTGAACG AGCCAAAGTC AATAAAGAGG ACATGAGCAC AAAACTGCCT 
CCTCTTAATA GTGATATTGG CAGCAGCAAT GCTAATGTTC CTGATCTGAT GGATGAGTTT 
ATAGCAGAAC GACTTCGAAG TGGTAATGCC TCAACTATGA CACGAAGAGG AAGTAGTCCA 
GGTAGCCTCG AAATTCCCAA AGACCTCCCT GATATTCTAA ATAAGCAAAA CCAAATGCGT 
CCTATTGATG ACCCAGGTGT ACCCTCAGAA TGGACTTCTC CTGCCAGTGC AGGGAGCAGT 
GATCTTATCA GCTCAGATAG TCATTCGGAT TCTTTCAGCG CTTTCCAGTA TGATGGCCGA 
AAATTTGACA ATTTTGGTTT TGGAGCTGAT ACTGGGAATG CATCCTCTGC TGACATGGAT 
TCAGGTTCTG GCCATTATCA GAGTGCTGAA GAGCAAGAGG TGGCTAGTCT AACCACACTA 
CACATTGACT CAGAAACAAG CAGTCTTAAT CAACAAGCTT TCTGTACTGA AGTTGCAACG 
GTTACTGGTT CAGAAAGTGC CTCTCCAGTC CATTCAGCTC TGGGATCCAG GTCACAGACA 
CCTTCTCCTT CCACACTGAA TATAGATCAC ATGGAACAGA AGGATCTACA GCTCGATGAG 
AAGCTCCACC ACTCTGTTCT TCAGACACCA GATGACCTAG AAATCAGTGA ATTTCCATCT 
GAATGCTGTA GTGTGATGGC AGGGGGCACT CTTACAGGAT GGCATGCTGA TGTTGCTACT 
GTAATGTGGC GAAGGATGCT GGGTATTTTG GGAGATGTCA ACGCTATTAT GGATCCTGAA 
ATACATGCTC AAGTTTTTGA TTACCTCTGT GAACTTTGGC AGAATCTAGC TAAGATTAGA 
GATAATCTTG GCATTTCAAC TGATAACCTG ACCTCCCCTT CTCCACCGGT TTTAATTCCT 
CCACTGAGAA TTCTTACACC TTGGCTTTTT AAGGCAACCA TGTTAACTGA TAAATATAAA 
CAAGGTAAAT TACATGCCTA TAAACTTATT TGTAATACAA TGAAAAGAAG ACAAGATGTT 
TCTCCAAATA GAGATTTTCT AACACATTTC TACAACATAA TGCATTGTGG ATTGCTTCAC 
ATTGATCAGG ATATTGTCAA TACAATCATC AAGCACTGCT CACCTCAATT TTTCTCACTT 
GGTTTGCCTG GTGCAACAAT GCTTATTATG GATTTTATTG TAGCAGCTGG TAGAGTGGCT 
TCTTCAGCTT TTCTCAATGC ACCAAGAGTG GAAGCACAAG TTCTTCTGGG ATCTTTGGTT 
TGCTTTCCCA ACTTGTATTG TGAACTGCCT GCTCTCCATC CCAACATCCC TGATATTGCT 
GTGTCTCAAT TTACAGATGT TAAGGAACTT ATAATCAAAA CTGTGTTAAG CTCTGCAAGA 
GATGAGCCCT CTGGTCCTGC ACGATGTGTA GCACTTTGCA GTTTAGGTAT TTGGATTTGT 
GAAGAACTAG TCCATGAGTC TCATCATCCT CAAATTAAGG AAGCTCTGAA TGTAATTTGT 
GTTTCCTTAA AGTTTACTAA TAAAACAGTA GCCCATGTAG CTTGTAACAT GCTTCACATG 
CTGGTTCATT ATGTACCTAG ACTTCAGATT TACCAGCCTG ATTCTCCCTT GAAAATTATT 
CAAATTCTAA TAGCCACTAT TACCCATCTT TTGCCAAGTA CGGAAGCGTC ATCTTATGAA 
ATGGACAAGA GGTTGGTGGT ATCCTTACTT CTGTGTCTTC TGGACTGGAT CATGGCCTTA 
CCTCTTAAGA CACTGCTCCA ACCAGTCCAT GCTACAGGAG CAGAAAATGA TAAAATAGAA 
AAATCTGTTC TTAACTGCAT TTATAAGGTA TTACATGGGT GTGTTTATGG AGCTCAGTGT 
TTTAGCAATC CGAGGTATTT TCCAATAAGC CTTTCTGATT TGGCATCTGT AGATTATGAT 
CCTTTTATGC ATTTGGAAAG CCTAAAAGAG CCTGAACCTT TGCACTCTCC TGATTCGGAA 
CGATCTTCTA AACTCCAGCC AGTAACAGAA GTGAAAACTC AGATGCAGCA AGGATTAATC 
TCCATAGCTG CCCGCACTGT TATCACACAT CTGGTGAATC ACTTGGGCCA TTATCCAATG 
AGCGGTGGTC CTGCTATGTT AACAAGTCAG GTGTGTGAGA ATCACGACAA TCATTACAGT 
GAAAGCACTG AACTTTCCCC TGAACTCTTC GAGAGTCCAA ATATTCAGTT TTTTGTGTTA 
AACAATACAA CCTTAGTGTC CTGTATCCAG ATCAGAGCTG AAGAGAGTAT ACCTGGAGGA 
GGTTTATCTG CTGGTCTTGC ATCAGCCAAT TCAAATGTCA GAATTATAGT ACGTGATCTT 
TCTGGAAAAT ATTCATGGGA TTCTGCCATC CTGTATGGAC CACCTCTTGT AAGTGGTCTG 
TCAGAACCTA CATCTCTCGT ACTTTCATTG TCTCACCAAG AGAAGCCAGA AGAACCTTCT 
GCATCTAATG AATGCTTAGA AGATATAACA GTTAAAGATG GGATTTCCCT CCAGTTTAAA 
AGAAGTTTTA GAGAAACTGT ACCAACTTGG GACACAATCA GAGATGAAGA AGATGTTCTT 
GATGAGCTTT TGCAGTATTT AGGTGTAACC AGTCCTGAAT GCTTACAGAG AACTGGAGTC 
TCACTTAATA TTCCTGCTCC ACAGCCTGTG TGCATTTCTG AAAAACAGGA GAATGATGTT 
ATTAATGCTA TCCTTAAGCA GCATACAGAG GAAAAAGAAT TTGTTGAGAA ACACTTTAAT 
GACTTAAACA TGAAAGCTGC AGAACAAGAT GAACCAACAC CTCAGAAACC TCAGTCAGCA 
TTTTATTATT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCCTGGGA CAAAAGGAGG 
AGCTTTCATC TCCTGAAGAA AAATGAAAAA CTACTTAGAG AACTTAGGAA CTTGGACTCA 
AGACAGTGCC GAGAGACACA CAAGATTGCA GTATTTTATG TTGCTGAAGG ACAAGAAGAC 
AAACATTCCA TTCTCACCAA TACAGGAGGA AGTCAAGCAT ATGAAGATTT TGTAGCTGGT 
CTTGGTTGGG AGGTAAATCT TACAAACCAT TGTGGTTTCA TGGGAGGACT ACAAAAAAAT 
AAAAGCACTG GACTGACCAC ACCGTATTTT GCTACCTCTA CAGTTGAAGT AATATTTCAT 
GTGTCAACAA GAATGCCTTC AGATTCTGAT GACTCTTTGA CCAAAAAATT GAGACATTTA 
GGAAATGATG AAGTGCACAT TGTTTGGTCA GAGCATACTA GAGACTACAG GAGAGGAATT 
ATTCCTACAG AATTTGGTGA TGTCCTTATT GTTATATACC CGATGAAAAA CCACATGTTC 
AGTATCCAGA TAATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCACTCTT TGATGGTGCT 
ATTGTGAATG GAAAGGTCCT ACCTATTATG GTTCGAGCAA CAGCTATAAA TGCAAGCCGT 
GCTCTGAAAT CCCTGATTCC ATTGTATCAA AACTTCTATG AGGAGAGAGC ACGATATCTG 
CAAACGATTG TCCAGCACCA TTTAGAGCCA ACGACATTTG AAGATTTTGC AGCACAGGTT 
TTTTCTCCAG CTCCCTACCA TCATTTACCA TCTGATGCCG TGGGCTCCTA CCCAGAGATC 
CTATCCAGTG AAACTCCCAC AGCGGCGCAG GTAGACGGGG CCGACCTGGC CTCTCCAATG 
TCTCCTCGAA CGAGCAAAAG CCGCATGTCC ATGAAGCTAC GTCGCTCCTC TGGCTCCGCC 
AATAAGTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014957496.1
CDS	208..6159
Translation	MFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILSSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_015102010.1
Organism	Ovis aries(sheep)
Definition	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015102010.1

ORF Insert Sequence:

ATGTTTTCAT GCTTAATCCC TGGATTTTCA GCACCACAGT CTGAATATGG ACCTCGAACT 
TTAGATAACC TCATTAATCC TCCACTCAAC CTACAAGAAA CTCAAGTCAC TATAGAGGAA 
ATCACTCCAC TTGTCCCCCC ACAATCAGGA GATAAAGGAC AAGAAGATCT TACAAGCTAT 
TTTCTTGAAG CACTTTTAAA ATATATAGTA ATTCAGGTAA AAAGTTTAGA GTGGAAGAAC 
AAAGAAAACC AAGAAAAAGG ATTTTCATTT CTGTTTTCAC ATTTTAAGAA ATATTATTTG 
CCTTATATTT TCCCAAACAT CTGTAAGGAG AATAGTTTAT ATCATCCTGT ACTTGATATC 
CCACAGATGA GACCAAAGCC CCATTATGTC ATGATAAAGA AGGATGCTGA AACCAATGAG 
GCAATCTATT GTACAAAGGA GCCTTTCATT AAGGCTCGTG TTATTGTCAT TCGTTGGCTG 
GTTTCATTCT GGCTGGAGCC AAAGCCGCAT ACAGGACCTC ATATTCCTGG GATGGAAGGT 
GAAGTCTTGC CAAAAAATAT TCAGAGAGCA GCTGCTAGTT TGGTATCCAG AGAAGAAAAC 
AAAAATGATA ATGCTGATAA AACAGATAGA AGTACAGAAC CTGAACAGTC TCATTCCAAC 
ACAAGCACTC TCACGGAGCG AGAACCTAGC TCTTCTAGTC TCTGTAGTAT TGATGAAGAG 
CATCTCACAG ACATTGAAAT AGTTCGCCGA GTTTTTTCTT CTAAAAGAAG TAACGTAAAC 
TTTGTCACAG AGATATTTCG TCAGGCATTT TTATTACCAA TTTGCGAAGC AGCAGCTATG 
AGAAAAGTGG TAAAAGTATA TCAAGAATGG ATCCAGCAAG AGGAAAAACC TTTGTTCATG 
CAAGAGCCTG AAGAAATTGT GATCTCTTCT TCAGACCTTC CGTGCATTGA CAATGTCACA 
GACCATGATA TTTCAATGGA AGAAGGAGAA AAAAGAGAAG AGGAAAATGG GACCAATGTT 
GCTGATCATG TTCGAAATTC CACTTGGCCT AAAAATGGAA CCTACCAAGA TATTCTTCAT 
AATGCTTCTG AAGAAGCCAT AGAACAAAAC ATACGAGCTG GTGCCCAGGC AGTTTTGCAG 
GTGTTTATTA TAAATTCTTC AAACATATTT CTTCTTGAAC CTGCAAATGA GATAAAAAGT 
CTTCTGGATG AGCACACAGA TATGTGTAAA CGAATTCTTA ACATTTATCG GTTCATGGTT 
GTGCAAGTAT CAATGGACAA AAAGACTTGG GAACAGATGC TGCTTGTGTT GCTCAGAGTC 
ACGGAGTCTG TACTGAAGAT GCCATCACAA GCTTTTCTAC AGTTCCAGGG GAAAAAAAAC 
ATGACTTTGG CAGGTCGACT TGCAGGACCA CTTTTCCAGA CTCTTATAGT TGCCTGGATT 
AAAGCAAATC TAAATGTGTA CATCTCTCGA GAACTTTGGG ATGACTTACT CTCAGTATTG 
TCATCGTTGA CCTATTGGGA AGAGTTAGCC ACTGAATGGT CGCTAACTAT GGAGACACTA 
ACTAAAGTTT TAGCTAGAAA TTTGTATAGT TTGGATCTCA GTGATTTACC ATTGGATAAG 
CTGAGTGAAC AGAAACAAAA AAAGCACAAA GGGAAAGGAG TGGGACATGA ATTTCAGAAA 
GTTTCAGTTG ACAAGTCATT TTCTAGAGGA TGGAGTCGCG ATCAGCCTGG CCAAGCCCCA 
ATGAGGCAGA GAAGTGCAAC AACCACTGGT TCCCCAGGCA CAGAAAAGGC AAGGAGTATA 
GTACGGCAAA AAACTGTTGC CATGAGAAGC CGATCCATTG GTGAATGTGC TCTGCCATCG 
GCCTATATAC GCAGTGCTAA AAGTGCTCCT GTTCTGATCC ATACTTCCAA ACCCTTCTTG 
CCTGATATTG TTCTCACTCC CCTTTCTGAT GAGCTTTCAG ATATTGATGA TGCTCAAATA 
CTTCCTCGTC CAACTAGAGT CAGACATTTT TCGCAAAGTG AAGACACTGG AAATGAAGTT 
TTTGGTGCTT TGAGTGAGGA GCAGCCATTG CCTCGAAGTA GCAGCACTTC TGACATCTTG 
GAACCATTCA CTGTTGAACG AGCCAAAGTC AATAAAGAGG ACATGAGCAC AAAACTGCCT 
CCTCTTAATA GTGATATTGG CAGCAGCAAT GCTAATGTTC CTGATCTGAT GGATGAGTTT 
ATAGCAGAAC GACTTCGAAG TGGTAATGCC TCAACTATGA CACGAAGAGG AAGTAGTCCA 
GGTAGCCTCG AAATTCCCAA AGACCTCCCT GATATTCTAA ATAAGCAAAA CCAAATGCGT 
CCTATTGATG ACCCAGGTGT ACCCTCAGAA TGGACTTCTC CTGCCAGTGC AGGGAGCAGT 
GATCTTATCA GCTCAGATAG TCATTCGGAT TCTTTCAGCG CTTTCCAGTA TGATGGCCGA 
AAATTTGACA ATTTTGGTTT TGGAGCTGAT ACTGGGAATG CATCCTCTGC TGACATGGAT 
TCAGGTTCTG GCCATTATCA GAGTGCTGAA GAGCAAGAGG TGGCTAGTCT AACCACACTA 
CACATTGACT CAGAAACAAG CAGTCTTAAT CAACAAGCTT TCTGTACTGA AGTTGCAACG 
GTTACTGGTT CAGAAAGTGC CTCTCCAGTC CATTCAGCTC TGGGATCCAG GTCACAGACA 
CCTTCTCCTT CCACACTGAA TATAGATCAC ATGGAACAGA AGGATCTACA GCTCGATGAG 
AAGCTCCACC ACTCTGTTCT TCAGACACCA GATGACCTAG AAATCAGTGA ATTTCCATCT 
GAATGCTGTA GTGTGATGGC AGGGGGCACT CTTACAGGAT GGCATGCTGA TGTTGCTACT 
GTAATGTGGC GAAGGATGCT GGGTATTTTG GGAGATGTCA ACGCTATTAT GGATCCTGAA 
ATACATGCTC AAGTTTTTGA TTACCTCTGT GAACTTTGGC AGAATCTAGC TAAGATTAGA 
GATAATCTTG GCATTTCAAC TGATAACCTG ACCTCCCCTT CTCCACCGGT TTTAATTCCT 
CCACTGAGAA TTCTTACACC TTGGCTTTTT AAGGCAACCA TGTTAACTGA TAAATATAAA 
CAAGGTAAAT TACATGCCTA TAAACTTATT TGTAATACAA TGAAAAGAAG ACAAGATGTT 
TCTCCAAATA GAGATTTTCT AACACATTTC TACAACATAA TGCATTGTGG ATTGCTTCAC 
ATTGATCAGG ATATTGTCAA TACAATCATC AAGCACTGCT CACCTCAATT TTTCTCACTT 
GGTTTGCCTG GTGCAACAAT GCTTATTATG GATTTTATTG TAGCAGCTGG TAGAGTGGCT 
TCTTCAGCTT TTCTCAATGC ACCAAGAGTG GAAGCACAAG TTCTTCTGGG ATCTTTGGTT 
TGCTTTCCCA ACTTGTATTG TGAACTGCCT GCTCTCCATC CCAACATCCC TGATATTGCT 
GTGTCTCAAT TTACAGATGT TAAGGAACTT ATAATCAAAA CTGTGTTAAG CTCTGCAAGA 
GATGAGCCCT CTGGTCCTGC ACGATGTGTA GCACTTTGCA GTTTAGGTAT TTGGATTTGT 
GAAGAACTAG TCCATGAGTC TCATCATCCT CAAATTAAGG AAGCTCTGAA TGTAATTTGT 
GTTTCCTTAA AGTTTACTAA TAAAACAGTA GCCCATGTAG CTTGTAACAT GCTTCACATG 
CTGGTTCATT ATGTACCTAG ACTTCAGATT TACCAGCCTG ATTCTCCCTT GAAAATTATT 
CAAATTCTAA TAGCCACTAT TACCCATCTT TTGCCAAGTA CGGAAGCGTC ATCTTATGAA 
ATGGACAAGA GGTTGGTGGT ATCCTTACTT CTGTGTCTTC TGGACTGGAT CATGGCCTTA 
CCTCTTAAGA CACTGCTCCA ACCAGTCCAT GCTACAGGAG CAGAAAATGA TAAAATAGAA 
AAATCTGTTC TTAACTGCAT TTATAAGGTA TTACATGGGT GTGTTTATGG AGCTCAGTGT 
TTTAGCAATC CGAGGTATTT TCCAATAAGC CTTTCTGATT TGGCATCTGT AGATTATGAT 
CCTTTTATGC ATTTGGAAAG CCTAAAAGAG CCTGAACCTT TGCACTCTCC TGATTCGGAA 
CGATCTTCTA AACTCCAGCC AGTAACAGAA GTGAAAACTC AGATGCAGCA AGGATTAATC 
TCCATAGCTG CCCGCACTGT TATCACACAT CTGGTGAATC ACTTGGGCCA TTATCCAATG 
AGCGGTGGTC CTGCTATGTT AACAAGTCAG GTGTGTGAGA ATCACGACAA TCATTACAGT 
GAAAGCACTG AACTTTCCCC TGAACTCTTC GAGAGTCCAA ATATTCAGTT TTTTGTGTTA 
AACAATACAA CCTTAGTGTC CTGTATCCAG ATCAGAGCTG AAGAGAGTAT ACCTGGAGGA 
GGTTTATCTG CTGGTCTTGC ATCAGCCAAT TCAAATGTCA GAATTATAGT ACGTGATCTT 
TCTGGAAAAT ATTCATGGGA TTCTGCCATC CTGTATGGAC CACCTCTTGT AAGTGGTCTG 
TCAGAACCTA CATCTCTCGT ACTTTCATTG TCTCACCAAG AGAAGCCAGA AGAACCTTCT 
GCATCTAATG AATGCTTAGA AGATATAACA GTTAAAGATG GGATTTCCCT CCAGTTTAAA 
AGAAGTTTTA GAGAAACTGT ACCAACTTGG GACACAATCA GAGATGAAGA AGATGTTCTT 
GATGAGCTTT TGCAGTATTT AGGTGTAACC AGTCCTGAAT GCTTACAGAG AACTGGAGTC 
TCACTTAATA TTCCTGCTCC ACAGCCTGTG TGCATTTCTG AAAAACAGGA GAATGATGTT 
ATTAATGCTA TCCTTAAGCA GCATACAGAG GAAAAAGAAT TTGTTGAGAA ACACTTTAAT 
GACTTAAACA TGAAAGCTGC AGAACAAGAT GAACCAACAC CTCAGAAACC TCAGTCAGCA 
TTTTATTATT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCCTGGGA CAAAAGGAGG 
AGCTTTCATC TCCTGAAGAA AAATGAAAAA CTACTTAGAG AACTTAGGAA CTTGGACTCA 
AGACAGTGCC GAGAGACACA CAAGATTGCA GTATTTTATG TTGCTGAAGG ACAAGAAGAC 
AAACATTCCA TTCTCACCAA TACAGGAGGA AGTCAAGCAT ATGAAGATTT TGTAGCTGGT 
CTTGGTTGGG AGGTAAATCT TACAAACCAT TGTGGTTTCA TGGGAGGACT ACAAAAAAAT 
AAAAGCACTG GACTGACCAC ACCGTATTTT GCTACCTCTA CAGTTGAAGT AATATTTCAT 
GTGTCAACAA GAATGCCTTC AGATTCTGAT GACTCTTTGA CCAAAAAATT GAGACATTTA 
GGAAATGATG AAGTGCACAT TGTTTGGTCA GAGCATACTA GAGACTACAG GAGAGGAATT 
ATTCCTACAG AATTTGGTGA TGTCCTTATT GTTATATACC CGATGAAAAA CCACATGTTC 
AGTATCCAGA TAATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCACTCTT TGATGGTGCT 
ATTGTGAATG GAAAGGTCCT ACCTATTATG GTTCGAGCAA CAGCTATAAA TGCAAGCCGT 
GCTCTGAAAT CCCTGATTCC ATTGTATCAA AACTTCTATG AGGAGAGAGC ACGATATCTG 
CAAACGATTG TCCAGCACCA TTTAGAGCCA ACGACATTTG AAGATTTTGC AGCACAGGTT 
TTTTCTCCAG CTCCCTACCA TCATTTACCA TCTGATGCCG TGGGCTCCTA CCCAGAGATC 
CTATCCAGTG AAACTCCCAC AGCGGCGCAG GTAGACGGGG CCGACCTGGC CTCTCCAATG 
TCTCCTCGAA CGAGCAAAAG CCGCATGTCC ATGAAGCTAC GTCGCTCCTC TGGCTCCGCC 
AATAAGTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_012130791.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986181.1 Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X17, mRNA.

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CloneID	OOv14054
Clone ID Related Accession (Same CDS sequence)	XM_012130791.2
Accession Version	XM_012130791.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	ral GTPase-activating protein subunit alpha-1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942367.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012130791.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCGCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAATGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTGACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GCCATGAGAA GCCGATCCAT TGGTGAATGT 
GCTCTGCCAT CGGCCTATAT ACGCAGTGCT AAAAGTGCTC CTGTTCTGAT CCATACTTCC 
AAACCCTTCT TGCCTGATAT TGTTCTCACT CCCCTTTCTG ATGAGCTTTC AGATATTGAT 
GATGCTCAAA TACTTCCTCG TCCAACTAGA GTCAGACATT TTTCGCAAAG TGAAGACACT 
GGAAATGAAG TTTTTGGTGC TTTGAGTGAG GAGCAGCCAT TGCCTCGAAG TAGCAGCACT 
TCTGACATCT TGGAACCATT CACTGTTGAA CGAGCCAAAG GTGCAGTTCC TGTCATTGAC 
AGTTCATCCC GTCATGCACC AAGCTTGCAG AGTTCCACAG AGGCTTCTTC AATAACTAGA 
TCCACTGAGA GCCACATCAC TGATACTCAT AGTAGAGAGT CTTCTCTGGA AGTTGGTGAT 
AGCATATATG ACCATCTCTG TCACTTAATA GATCCAGTAG AACTTGCAGA TTCAGCTTTT 
GAACAAATCC AGTACATTGA CCTTGAAGGA GATGATGATC TTCTTTCCTC CCTGAAAGAA 
TATTTTAAGG AAACCCAAGA AAGTCACAGT AAAAATGAGT TAGATAAAGA TGCAGCTTCG 
CAGGAAGTGA TCTCTGTTGC AGTAAATAAG GATGAAAGAT CATCCTCAGA TAAATTAGAG 
TACATAGACC AGGAAAATAA ATCAGAAAAT ACCACCTCTT TTGTTGGAAC TCCTGAAAAT 
ATACAATTTC AAAAAGAACC AAACTCTACT GTCTTCACAA ATATTAGTGC ACCCAACCAG 
TTAGACAGCT TTACGAAAAC AAAAACTTCT GAAAAATGTA AACAACTAAA CAATGATAAA 
CAGTCATCGG AACCTAGTTC AGGTAGCCCT TGTGATAAAG AAAAGAGAAA ACATCTGTAT 
AGACAAACAG CCACAGAATT AGATGCCTGT ATAGATACCA CACTAGCTGA AAAGGTGAAG 
GGTGTGCAAA TTAATGAAAA AATCACTGTC CCACATGTTT TGCATGCCAA GAAAACTACA 
CTAAAAGCAC CTGTTAACCG TAGAATGCCT CATGTTACAA GCACTAGCAA GCTTTCTCCA 
ACTAAAAGGA GCTTGTCTGA AACAGTAACT CACAGAGCCA AAATCATGAA AATTGCTACT 
AAAAAGCGAA ATAGTGTTCA TGTTACTTTT AGACCTTCTA CTGAGTCAGT TCAGTTTTAT 
AATCCTCTGG AAAACAAAGA GGCTCCATGG AAGATGAGAC TGCGGAAGCT CAGTGGCTTT 
AGTGGTAGTA ATAGCAACAT CAGCAACCCC CACACCAGCT CCAACAGTGC TGCAGAGCTA 
GTAAAACCTG GTGTGTACAG GCCTCTAGAT ACAATTAGTG CAGCATCAGC AAGCAGTGAA 
ACAATTAAGG AAACGACAGA GATTCCCACC GCCATATTAC AGAAAGAAGG AATTGCAACT 
AATCAGTTAG GTAGTCGTAG TACTATTAAG TCATCAAGTC ACGAGGCAGG ACTACCGCAG 
GGCTCCCTGG GTGGCGTTTA TAAAACTGTT GTCCATGCTC TTTCGAAGCC GAAGGCAAAT 
GTTTCCCCAC AGAGGCAGAA CAGAATGCCA CCAGAGGCTC CACTGAGGGA TCTGTACAGT 
CATGTAATGG GCTATTTTGG AAGGAAAGCT GCAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAGCCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGTGGG CTCCTACCCA 
GAGATCCTAT CCAGTGAAAC TCCCACAGCG GCGCAGGTAG ACGGGGCCGA CCTGGCCTCT 
CCAATGTCTC CTCGAACGAG CAAAAGCCGC ATGTCCATGA AGCTGCGTCG CTCCTCTGGC 
TCCGCCAATA AGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986181.1
CDS	457..8274
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIGRIFTAEPPEKPREHENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQENKSENTTSFVGTPENIQFQKEPNSTVFTNISAPNQLDSFTKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDTTLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILSSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_012130791.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130791.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCGCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAATGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTGACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GCCATGAGAA GCCGATCCAT TGGTGAATGT 
GCTCTGCCAT CGGCCTATAT ACGCAGTGCT AAAAGTGCTC CTGTTCTGAT CCATACTTCC 
AAACCCTTCT TGCCTGATAT TGTTCTCACT CCCCTTTCTG ATGAGCTTTC AGATATTGAT 
GATGCTCAAA TACTTCCTCG TCCAACTAGA GTCAGACATT TTTCGCAAAG TGAAGACACT 
GGAAATGAAG TTTTTGGTGC TTTGAGTGAG GAGCAGCCAT TGCCTCGAAG TAGCAGCACT 
TCTGACATCT TGGAACCATT CACTGTTGAA CGAGCCAAAG GTGCAGTTCC TGTCATTGAC 
AGTTCATCCC GTCATGCACC AAGCTTGCAG AGTTCCACAG AGGCTTCTTC AATAACTAGA 
TCCACTGAGA GCCACATCAC TGATACTCAT AGTAGAGAGT CTTCTCTGGA AGTTGGTGAT 
AGCATATATG ACCATCTCTG TCACTTAATA GATCCAGTAG AACTTGCAGA TTCAGCTTTT 
GAACAAATCC AGTACATTGA CCTTGAAGGA GATGATGATC TTCTTTCCTC CCTGAAAGAA 
TATTTTAAGG AAACCCAAGA AAGTCACAGT AAAAATGAGT TAGATAAAGA TGCAGCTTCG 
CAGGAAGTGA TCTCTGTTGC AGTAAATAAG GATGAAAGAT CATCCTCAGA TAAATTAGAG 
TACATAGACC AGGAAAATAA ATCAGAAAAT ACCACCTCTT TTGTTGGAAC TCCTGAAAAT 
ATACAATTTC AAAAAGAACC AAACTCTACT GTCTTCACAA ATATTAGTGC ACCCAACCAG 
TTAGACAGCT TTACGAAAAC AAAAACTTCT GAAAAATGTA AACAACTAAA CAATGATAAA 
CAGTCATCGG AACCTAGTTC AGGTAGCCCT TGTGATAAAG AAAAGAGAAA ACATCTGTAT 
AGACAAACAG CCACAGAATT AGATGCCTGT ATAGATACCA CACTAGCTGA AAAGGTGAAG 
GGTGTGCAAA TTAATGAAAA AATCACTGTC CCACATGTTT TGCATGCCAA GAAAACTACA 
CTAAAAGCAC CTGTTAACCG TAGAATGCCT CATGTTACAA GCACTAGCAA GCTTTCTCCA 
ACTAAAAGGA GCTTGTCTGA AACAGTAACT CACAGAGCCA AAATCATGAA AATTGCTACT 
AAAAAGCGAA ATAGTGTTCA TGTTACTTTT AGACCTTCTA CTGAGTCAGT TCAGTTTTAT 
AATCCTCTGG AAAACAAAGA GGCTCCATGG AAGATGAGAC TGCGGAAGCT CAGTGGCTTT 
AGTGGTAGTA ATAGCAACAT CAGCAACCCC CACACCAGCT CCAACAGTGC TGCAGAGCTA 
GTAAAACCTG GTGTGTACAG GCCTCTAGAT ACAATTAGTG CAGCATCAGC AAGCAGTGAA 
ACAATTAAGG AAACGACAGA GATTCCCACC GCCATATTAC AGAAAGAAGG AATTGCAACT 
AATCAGTTAG GTAGTCGTAG TACTATTAAG TCATCAAGTC ACGAGGCAGG ACTACCGCAG 
GGCTCCCTGG GTGGCGTTTA TAAAACTGTT GTCCATGCTC TTTCGAAGCC GAAGGCAAAT 
GTTTCCCCAC AGAGGCAGAA CAGAATGCCA CCAGAGGCTC CACTGAGGGA TCTGTACAGT 
CATGTAATGG GCTATTTTGG AAGGAAAGCT GCAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAGCCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGTGGG CTCCTACCCA 
GAGATCCTAT CCAGTGAAAC TCCCACAGCG GCGCAGGTAG ACGGGGCCGA CCTGGCCTCT 
CCAATGTCTC CTCGAACGAG CAAAAGCCGC ATGTCCATGA AGCTGCGTCG CTCCTCTGGC 
TCCGCCAATA AGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_012130792.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986182.1 Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X18, mRNA.

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CloneID	OOv14055
Clone ID Related Accession (Same CDS sequence)	XM_012130792.2
Accession Version	XM_012130792.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	ral GTPase-activating protein subunit alpha-1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942367.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012130792.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCGCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAATGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTGACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GCCATGAGAA GCCGATCCAT TGGTGAATGT 
GCTCTGCCAT CGGCCTATAT ACGCAGTGCT AAAAGTGCTC CTGTTCTGAT CCATACTTCC 
AAACCCTTCT TGCCTGATAT TGTTCTCACT CCCCTTTCTG ATGAGCTTTC AGATATTGAT 
GATGCTCAAA TACTTCCTCG TCCAACTAGA GTCAGACATT TTTCGCAAAG TGAAGACACT 
GGAAATGAAG TTTTTGGTGC TTTGAGTGAG GAGCAGCCAT TGCCTCGAAG TAGCAGCACT 
TCTGACATCT TGGAACCATT CACTGTTGAA CGAGCCAAAG GTGCAGTTCC TGTCATTGAC 
AGTTCATCCC GTCATGCACC AAGCTTGCAG AGTTCCACAG AGGCTTCTTC AATAACTAGA 
TCCACTGAGA GCCACATCAC TGATACTCAT AGTAGAGAGT CTTCTCTGGA AGTTGGTGAT 
AGCATATATG ACCATCTCTG TCACTTAATA GATCCAGTAG AACTTGCAGA TTCAGCTTTT 
GAACAAATCC AGTACATTGA CCTTGAAGGA GATGATGATC TTCTTTCCTC CCTGAAAGAA 
TATTTTAAGG AAACCCAAGA AAGTCACAGT AAAAATGAGT TAGATAAAGA TGCAGCTTCG 
CAGGAAGTGA TCTCTGTTGC AGTAAATAAG GATGAAAGAT CATCCTCAGA TAAATTAGAG 
TACATAGACC AGGAAAATAA ATCAGAAAAT ACCACCTCTT TTGTTGGAAC TCCTGAAAAT 
ATACAATTTC AAAAAGAACC AAACTCTACT GTCTTCACAA ATATTAGTGC ACCCAACCAG 
TTAGACAGCT TTACGAAAAC AAAAACTTCT GAAAAATGTA AACAACTAAA CAATGATAAA 
CAGTCATCGG AACCTAGTTC AGGTAGCCCT TGTGATAAAG AAAAGAGAAA ACATCTGTAT 
AGACAAACAG CCACAGAATT AGATGCCTGT ATAGATACCA CACTAGCTGA AAAGGTGAAG 
GGTGTGCAAA TTAATGAAAA AATCACTGTC CCACATGTTT TGCATGCCAA GAAAACTACA 
CTAAAAGCAC CTGTTAACCG TAGAATGCCT CATGTTACAA GCACTAGCAA GCTTTCTCCA 
ACTAAAAGGA GCTTGTCTGA AACAGTAACT CACAGAGCCA AAATCATGAA AATTGCTACT 
AAAAAGCGAA ATAGTGTTCA TGTTACTTTT AGACCTTCTA CTGAGTCAGT TCAGTTTTAT 
AATCCTCTGG AAAACAAAGA GGCTCCATGG AAGATGAGAC TGCGGAAGCT CAGTGGCTTT 
AGTGGTAGTA ATAGCAACAT CAGCAACCCC CACACCAGCT CCAACAGTGC TGCAGAGCTA 
GTAAAACCTG GTGTGTACAG GCCTCTAGAT ACAATTAGTG CAGCATCAGC AAGCAGTGAA 
ACAATTAAGG AAACGACAGA GATTCCCACC GCCATATTAC AGAAAGAAGG AATTGCAACT 
AATCAGTTAG GTAGTCGTAG TACTATTAAG TCATCAAGTC ACGAGGCAGG ACTACCGCAG 
GGCTCCCTGG GTGGCGTTTA TAAAACTGTT GTCCATGCTC TTTCGAAGCC GAAGGCAAAT 
GTTTCCCCAC AGAGGCAGAA CAGAATGCCA CCAGAGGCTC CACTGAGGGA TCTGTACAGT 
CATGTAATGG GCTATTTTGG AAGGAAAGCT GCAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAGCCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGGCTC CTACCCAGAG 
ATCCTATCCA GTGAAACTCC CACAGCGGCG CAGGTAGACG GGGCCGACCT GGCCTCTCCA 
ATGTCTCCTC GAACGAGCAA AAGCCGCATG TCCATGAAGC TGCGTCGCTC CTCTGGCTCC 
GCCAATAAGT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986182.1
CDS	457..8271
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIGRIFTAEPPEKPREHENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQENKSENTTSFVGTPENIQFQKEPNSTVFTNISAPNQLDSFTKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDTTLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAGSYPEILSSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_012130792.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130792.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCGCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAATGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTGACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GCCATGAGAA GCCGATCCAT TGGTGAATGT 
GCTCTGCCAT CGGCCTATAT ACGCAGTGCT AAAAGTGCTC CTGTTCTGAT CCATACTTCC 
AAACCCTTCT TGCCTGATAT TGTTCTCACT CCCCTTTCTG ATGAGCTTTC AGATATTGAT 
GATGCTCAAA TACTTCCTCG TCCAACTAGA GTCAGACATT TTTCGCAAAG TGAAGACACT 
GGAAATGAAG TTTTTGGTGC TTTGAGTGAG GAGCAGCCAT TGCCTCGAAG TAGCAGCACT 
TCTGACATCT TGGAACCATT CACTGTTGAA CGAGCCAAAG GTGCAGTTCC TGTCATTGAC 
AGTTCATCCC GTCATGCACC AAGCTTGCAG AGTTCCACAG AGGCTTCTTC AATAACTAGA 
TCCACTGAGA GCCACATCAC TGATACTCAT AGTAGAGAGT CTTCTCTGGA AGTTGGTGAT 
AGCATATATG ACCATCTCTG TCACTTAATA GATCCAGTAG AACTTGCAGA TTCAGCTTTT 
GAACAAATCC AGTACATTGA CCTTGAAGGA GATGATGATC TTCTTTCCTC CCTGAAAGAA 
TATTTTAAGG AAACCCAAGA AAGTCACAGT AAAAATGAGT TAGATAAAGA TGCAGCTTCG 
CAGGAAGTGA TCTCTGTTGC AGTAAATAAG GATGAAAGAT CATCCTCAGA TAAATTAGAG 
TACATAGACC AGGAAAATAA ATCAGAAAAT ACCACCTCTT TTGTTGGAAC TCCTGAAAAT 
ATACAATTTC AAAAAGAACC AAACTCTACT GTCTTCACAA ATATTAGTGC ACCCAACCAG 
TTAGACAGCT TTACGAAAAC AAAAACTTCT GAAAAATGTA AACAACTAAA CAATGATAAA 
CAGTCATCGG AACCTAGTTC AGGTAGCCCT TGTGATAAAG AAAAGAGAAA ACATCTGTAT 
AGACAAACAG CCACAGAATT AGATGCCTGT ATAGATACCA CACTAGCTGA AAAGGTGAAG 
GGTGTGCAAA TTAATGAAAA AATCACTGTC CCACATGTTT TGCATGCCAA GAAAACTACA 
CTAAAAGCAC CTGTTAACCG TAGAATGCCT CATGTTACAA GCACTAGCAA GCTTTCTCCA 
ACTAAAAGGA GCTTGTCTGA AACAGTAACT CACAGAGCCA AAATCATGAA AATTGCTACT 
AAAAAGCGAA ATAGTGTTCA TGTTACTTTT AGACCTTCTA CTGAGTCAGT TCAGTTTTAT 
AATCCTCTGG AAAACAAAGA GGCTCCATGG AAGATGAGAC TGCGGAAGCT CAGTGGCTTT 
AGTGGTAGTA ATAGCAACAT CAGCAACCCC CACACCAGCT CCAACAGTGC TGCAGAGCTA 
GTAAAACCTG GTGTGTACAG GCCTCTAGAT ACAATTAGTG CAGCATCAGC AAGCAGTGAA 
ACAATTAAGG AAACGACAGA GATTCCCACC GCCATATTAC AGAAAGAAGG AATTGCAACT 
AATCAGTTAG GTAGTCGTAG TACTATTAAG TCATCAAGTC ACGAGGCAGG ACTACCGCAG 
GGCTCCCTGG GTGGCGTTTA TAAAACTGTT GTCCATGCTC TTTCGAAGCC GAAGGCAAAT 
GTTTCCCCAC AGAGGCAGAA CAGAATGCCA CCAGAGGCTC CACTGAGGGA TCTGTACAGT 
CATGTAATGG GCTATTTTGG AAGGAAAGCT GCAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAGCCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGGCTC CTACCCAGAG 
ATCCTATCCA GTGAAACTCC CACAGCGGCG CAGGTAGACG GGGCCGACCT GGCCTCTCCA 
ATGTCTCCTC GAACGAGCAA AAGCCGCATG TCCATGAAGC TGCGTCGCTC CTCTGGCTCC 
GCCAATAAGT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_012130793.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986183.1 Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X19, mRNA.

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CloneID	OOv14056
Clone ID Related Accession (Same CDS sequence)	XM_012130793.2
Accession Version	XM_012130793.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	ral GTPase-activating protein subunit alpha-1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942367.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012130793.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCGCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA TGAGTTAGAT 
AAAGATGCAG CTTCGCAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AATAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAATATACA ATTTCAAAAA GAACCAAACT CTACTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTACG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TACCACACTA 
GCTGAAAAGG TGAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCA 
TCAGCAAGCA GTGAAACAAT TAAGGAAACG ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC TCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAA CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTCCC TGATATTCTA 
AATAAGCAAA ACCAAATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAGT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCTGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATTGAC TCAGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG AAGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTGA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA GAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTCTG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAACATA 
ATGCATTGTG GATTGCTTCA CATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCAACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAACTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCACAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACGGAAGCGT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTT CTTAACTGCA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCTTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAGATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATCACACA TCTGGTGAAT 
CACTTGGGCC ATTATCCAAT GAGCGGTGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CTGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT GTCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATC 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GAACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTTAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACA 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCCTGGG ACAAAAGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGACTGACCA CACCGTATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA TGTGTCAACA AGAATGCCTT CAGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTTATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCCCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGATATCT GCAAACGATT GTCCAGCACC ATTTAGAGCC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GTGGGCTCCT ACCCAGAGAT CCTATCCAGT GAAACTCCCA CAGCGGCGCA GGTAGACGGG 
GCCGACCTGG CCTCTCCAAT GTCTCCTCGA ACGAGCAAAA GCCGCATGTC CATGAAGCTG 
CGTCGCTCCT CTGGCTCCGC CAATAAGTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986183.1
CDS	457..8229
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQENKSENTTSFVGTPENIQFQKEPNSTVFTNISAPNQLDSFTKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDTTLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILSSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_012130793.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130793.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCGCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA TGAGTTAGAT 
AAAGATGCAG CTTCGCAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AATAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAATATACA ATTTCAAAAA GAACCAAACT CTACTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTACG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TACCACACTA 
GCTGAAAAGG TGAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCA 
TCAGCAAGCA GTGAAACAAT TAAGGAAACG ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC TCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAA CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTCCC TGATATTCTA 
AATAAGCAAA ACCAAATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAGT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCTGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATTGAC TCAGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG AAGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTGA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA GAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTCTG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAACATA 
ATGCATTGTG GATTGCTTCA CATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCAACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAACTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCACAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACGGAAGCGT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTT CTTAACTGCA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCTTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAGATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATCACACA TCTGGTGAAT 
CACTTGGGCC ATTATCCAAT GAGCGGTGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CTGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT GTCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATC 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GAACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTTAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACA 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCCTGGG ACAAAAGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGACTGACCA CACCGTATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA TGTGTCAACA AGAATGCCTT CAGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTTATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCCCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGATATCT GCAAACGATT GTCCAGCACC ATTTAGAGCC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GTGGGCTCCT ACCCAGAGAT CCTATCCAGT GAAACTCCCA CAGCGGCGCA GGTAGACGGG 
GCCGACCTGG CCTCTCCAAT GTCTCCTCGA ACGAGCAAAA GCCGCATGTC CATGAAGCTG 
CGTCGCTCCT CTGGCTCCGC CAATAAGTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_012130795.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986185.1 Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X20, mRNA.

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CloneID	OOv14057
Clone ID Related Accession (Same CDS sequence)	XM_012130795.2
Accession Version	XM_012130795.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	ral GTPase-activating protein subunit alpha-1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942367.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012130795.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCGCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAATGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTGACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GATATTGATG ATGCTCAAAT ACTTCCTCGT 
CCAACTAGAG TCAGACATTT TTCGCAAAGT GAAGACACTG GAAATGAAGT TTTTGGTGCT 
TTGAGTGAGG AGCAGCCATT GCCTCGAAGT AGCAGCACTT CTGACATCTT GGAACCATTC 
ACTGTTGAAC GAGCCAAAGG TGCAGTTCCT GTCATTGACA GTTCATCCCG TCATGCACCA 
AGCTTGCAGA GTTCCACAGA GGCTTCTTCA ATAACTAGAT CCACTGAGAG CCACATCACT 
GATACTCATA GTAGAGAGTC TTCTCTGGAA GTTGGTGATA GCATATATGA CCATCTCTGT 
CACTTAATAG ATCCAGTAGA ACTTGCAGAT TCAGCTTTTG AACAAATCCA GTACATTGAC 
CTTGAAGGAG ATGATGATCT TCTTTCCTCC CTGAAAGAAT ATTTTAAGGA AACCCAAGAA 
AGTCACAGTA AAAATGAGTT AGATAAAGAT GCAGCTTCGC AGGAAGTGAT CTCTGTTGCA 
GTAAATAAGG ATGAAAGATC ATCCTCAGAT AAATTAGAGT ACATAGACCA GGAAAATAAA 
TCAGAAAATA CCACCTCTTT TGTTGGAACT CCTGAAAATA TACAATTTCA AAAAGAACCA 
AACTCTACTG TCTTCACAAA TATTAGTGCA CCCAACCAGT TAGACAGCTT TACGAAAACA 
AAAACTTCTG AAAAATGTAA ACAACTAAAC AATGATAAAC AGTCATCGGA ACCTAGTTCA 
GGTAGCCCTT GTGATAAAGA AAAGAGAAAA CATCTGTATA GACAAACAGC CACAGAATTA 
GATGCCTGTA TAGATACCAC ACTAGCTGAA AAGGTGAAGG GTGTGCAAAT TAATGAAAAA 
ATCACTGTCC CACATGTTTT GCATGCCAAG AAAACTACAC TAAAAGCACC TGTTAACCGT 
AGAATGCCTC ATGTTACAAG CACTAGCAAG CTTTCTCCAA CTAAAAGGAG CTTGTCTGAA 
ACAGTAACTC ACAGAGCCAA AATCATGAAA ATTGCTACTA AAAAGCGAAA TAGTGTTCAT 
GTTACTTTTA GACCTTCTAC TGAGTCAGTT CAGTTTTATA ATCCTCTGGA AAACAAAGAG 
GCTCCATGGA AGATGAGACT GCGGAAGCTC AGTGGCTTTA GTGGTAGTAA TAGCAACATC 
AGCAACCCCC ACACCAGCTC CAACAGTGCT GCAGAGCTAG TAAAACCTGG TGTGTACAGG 
CCTCTAGATA CAATTAGTGC AGCATCAGCA AGCAGTGAAA CAATTAAGGA AACGACAGAG 
ATTCCCACCG CCATATTACA GAAAGAAGGA ATTGCAACTA ATCAGTTAGG TAGTCGTAGT 
ACTATTAAGT CATCAAGTCA CGAGGCAGGA CTACCGCAGG GCTCCCTGGG TGGCGTTTAT 
AAAACTGTTG TCCATGCTCT TTCGAAGCCG AAGGCAAATG TTTCCCCACA GAGGCAGAAC 
AGAATGCCAC CAGAGGCTCC ACTGAGGGAT CTGTACAGTC ATGTAATGGG CTATTTTGGA 
AGGAAAGCTG CAGTCAATAA AGAGGACATG AGCACAAAAC TGCCTCCTCT TAATAGTGAT 
ATTGGCAGCA GCAATGCTAA TGTTCCTGAT CTGATGGATG AGTTTATAGC AGAACGACTT 
CGAAGTGGTA ATGCCTCAAC TATGACACGA AGAGGAAGTA GTCCAGGTAG CCTCGAAATT 
CCCAAAGACC TCCCTGATAT TCTAAATAAG CAAAACCAAA TGCGTCCTAT TGATGACCCA 
GGTGTACCCT CAGAATGGAC TTCTCCTGCC AGTGCAGGGA GCAGTGATCT TATCAGCTCA 
GATAGTCATT CGGATTCTTT CAGCGCTTTC CAGTATGATG GCCGAAAATT TGACAATTTT 
GGTTTTGGAG CTGATACTGG GAATGCATCC TCTGCTGACA TGGATTCAGG TTCTGGCCAT 
TATCAGAGTG CTGAAGAGCA AGAGGTGGCT AGTCTAACCA CACTACACAT TGACTCAGAA 
ACAAGCAGTC TTAATCAACA AGCTTTCTGT ACTGAAGTTG CAACGGTTAC TGGTTCAGAA 
AGTGCCTCTC CAGTCCATTC AGCTCTGGGA TCCAGGTCAC AGACACCTTC TCCTTCCACA 
CTGAATATAG ATCACATGGA ACAGAAGGAT CTACAGCTCG ATGAGAAGCT CCACCACTCT 
GTTCTTCAGA CACCAGATGA CCTAGAAATC AGTGAATTTC CATCTGAATG CTGTAGTGTG 
ATGGCAGGGG GCACTCTTAC AGGATGGCAT GCTGATGTTG CTACTGTAAT GTGGCGAAGG 
ATGCTGGGTA TTTTGGGAGA TGTCAACGCT ATTATGGATC CTGAAATACA TGCTCAAGTT 
TTTGATTACC TCTGTGAACT TTGGCAGAAT CTAGCTAAGA TTAGAGATAA TCTTGGCATT 
TCAACTGATA ACCTGACCTC CCCTTCTCCA CCGGTTTTAA TTCCTCCACT GAGAATTCTT 
ACACCTTGGC TTTTTAAGGC AACCATGTTA ACTGATAAAT ATAAACAAGG TAAATTACAT 
GCCTATAAAC TTATTTGTAA TACAATGAAA AGAAGACAAG ATGTTTCTCC AAATAGAGAT 
TTTCTAACAC ATTTCTACAA CATAATGCAT TGTGGATTGC TTCACATTGA TCAGGATATT 
GTCAATACAA TCATCAAGCA CTGCTCACCT CAATTTTTCT CACTTGGTTT GCCTGGTGCA 
ACAATGCTTA TTATGGATTT TATTGTAGCA GCTGGTAGAG TGGCTTCTTC AGCTTTTCTC 
AATGCACCAA GAGTGGAAGC ACAAGTTCTT CTGGGATCTT TGGTTTGCTT TCCCAACTTG 
TATTGTGAAC TGCCTGCTCT CCATCCCAAC ATCCCTGATA TTGCTGTGTC TCAATTTACA 
GATGTTAAGG AACTTATAAT CAAAACTGTG TTAAGCTCTG CAAGAGATGA GCCCTCTGGT 
CCTGCACGAT GTGTAGCACT TTGCAGTTTA GGTATTTGGA TTTGTGAAGA ACTAGTCCAT 
GAGTCTCATC ATCCTCAAAT TAAGGAAGCT CTGAATGTAA TTTGTGTTTC CTTAAAGTTT 
ACTAATAAAA CAGTAGCCCA TGTAGCTTGT AACATGCTTC ACATGCTGGT TCATTATGTA 
CCTAGACTTC AGATTTACCA GCCTGATTCT CCCTTGAAAA TTATTCAAAT TCTAATAGCC 
ACTATTACCC ATCTTTTGCC AAGTACGGAA GCGTCATCTT ATGAAATGGA CAAGAGGTTG 
GTGGTATCCT TACTTCTGTG TCTTCTGGAC TGGATCATGG CCTTACCTCT TAAGACACTG 
CTCCAACCAG TCCATGCTAC AGGAGCAGAA AATGATAAAA TAGAAAAATC TGTTCTTAAC 
TGCATTTATA AGGTATTACA TGGGTGTGTT TATGGAGCTC AGTGTTTTAG CAATCCGAGG 
TATTTTCCAA TAAGCCTTTC TGATTTGGCA TCTGTAGATT ATGATCCTTT TATGCATTTG 
GAAAGCCTAA AAGAGCCTGA ACCTTTGCAC TCTCCTGATT CGGAACGATC TTCTAAACTC 
CAGCCAGTAA CAGAAGTGAA AACTCAGATG CAGCAAGGAT TAATCTCCAT AGCTGCCCGC 
ACTGTTATCA CACATCTGGT GAATCACTTG GGCCATTATC CAATGAGCGG TGGTCCTGCT 
ATGTTAACAA GTCAGGTGTG TGAGAATCAC GACAATCATT ACAGTGAAAG CACTGAACTT 
TCCCCTGAAC TCTTCGAGAG TCCAAATATT CAGTTTTTTG TGTTAAACAA TACAACCTTA 
GTGTCCTGTA TCCAGATCAG AGCTGAAGAG AGTATACCTG GAGGAGGTTT ATCTGCTGGT 
CTTGCATCAG CCAATTCAAA TGTCAGAATT ATAGTACGTG ATCTTTCTGG AAAATATTCA 
TGGGATTCTG CCATCCTGTA TGGACCACCT CTTGTAAGTG GTCTGTCAGA ACCTACATCT 
CTCGTACTTT CATTGTCTCA CCAAGAGAAG CCAGAAGAAC CTTCTGCATC TAATGAATGC 
TTAGAAGATA TAACAGTTAA AGATGGGATT TCCCTCCAGT TTAAAAGAAG TTTTAGAGAA 
ACTGTACCAA CTTGGGACAC AATCAGAGAT GAAGAAGATG TTCTTGATGA GCTTTTGCAG 
TATTTAGGTG TAACCAGTCC TGAATGCTTA CAGAGAACTG GAGTCTCACT TAATATTCCT 
GCTCCACAGC CTGTGTGCAT TTCTGAAAAA CAGGAGAATG ATGTTATTAA TGCTATCCTT 
AAGCAGCATA CAGAGGAAAA AGAATTTGTT GAGAAACACT TTAATGACTT AAACATGAAA 
GCTGCAGAAC AAGATGAACC AACACCTCAG AAACCTCAGT CAGCATTTTA TTATTGCAGA 
TTGCTTCTTA GTATATTGGG AATGAATTCC TGGGACAAAA GGAGGAGCTT TCATCTCCTG 
AAGAAAAATG AAAAACTACT TAGAGAACTT AGGAACTTGG ACTCAAGACA GTGCCGAGAG 
ACACACAAGA TTGCAGTATT TTATGTTGCT GAAGGACAAG AAGACAAACA TTCCATTCTC 
ACCAATACAG GAGGAAGTCA AGCATATGAA GATTTTGTAG CTGGTCTTGG TTGGGAGGTA 
AATCTTACAA ACCATTGTGG TTTCATGGGA GGACTACAAA AAAATAAAAG CACTGGACTG 
ACCACACCGT ATTTTGCTAC CTCTACAGTT GAAGTAATAT TTCATGTGTC AACAAGAATG 
CCTTCAGATT CTGATGACTC TTTGACCAAA AAATTGAGAC ATTTAGGAAA TGATGAAGTG 
CACATTGTTT GGTCAGAGCA TACTAGAGAC TACAGGAGAG GAATTATTCC TACAGAATTT 
GGTGATGTCC TTATTGTTAT ATACCCGATG AAAAACCACA TGTTCAGTAT CCAGATAATG 
AAAAAACCAG AGGTTCCCTT CTTTGGTCCA CTCTTTGATG GTGCTATTGT GAATGGAAAG 
GTCCTACCTA TTATGGTTCG AGCAACAGCT ATAAATGCAA GCCGTGCTCT GAAATCCCTG 
ATTCCATTGT ATCAAAACTT CTATGAGGAG AGAGCACGAT ATCTGCAAAC GATTGTCCAG 
CACCATTTAG AGCCAACGAC ATTTGAAGAT TTTGCAGCAC AGGTTTTTTC TCCAGCTCCC 
TACCATCATT TACCATCTGA TGCCGTGGGC TCCTACCCAG AGATCCTATC CAGTGAAACT 
CCCACAGCGG CGCAGGTAGA CGGGGCCGAC CTGGCCTCTC CAATGTCTCC TCGAACGAGC 
AAAAGCCGCA TGTCCATGAA GCTGCGTCGC TCCTCTGGCT CCGCCAATAA GTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986185.1
CDS	457..8133
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIGRIFTAEPPEKPREHENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQENKSENTTSFVGTPENIQFQKEPNSTVFTNISAPNQLDSFTKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDTTLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILSSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_012130795.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130795.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCGCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAATGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTGACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GATATTGATG ATGCTCAAAT ACTTCCTCGT 
CCAACTAGAG TCAGACATTT TTCGCAAAGT GAAGACACTG GAAATGAAGT TTTTGGTGCT 
TTGAGTGAGG AGCAGCCATT GCCTCGAAGT AGCAGCACTT CTGACATCTT GGAACCATTC 
ACTGTTGAAC GAGCCAAAGG TGCAGTTCCT GTCATTGACA GTTCATCCCG TCATGCACCA 
AGCTTGCAGA GTTCCACAGA GGCTTCTTCA ATAACTAGAT CCACTGAGAG CCACATCACT 
GATACTCATA GTAGAGAGTC TTCTCTGGAA GTTGGTGATA GCATATATGA CCATCTCTGT 
CACTTAATAG ATCCAGTAGA ACTTGCAGAT TCAGCTTTTG AACAAATCCA GTACATTGAC 
CTTGAAGGAG ATGATGATCT TCTTTCCTCC CTGAAAGAAT ATTTTAAGGA AACCCAAGAA 
AGTCACAGTA AAAATGAGTT AGATAAAGAT GCAGCTTCGC AGGAAGTGAT CTCTGTTGCA 
GTAAATAAGG ATGAAAGATC ATCCTCAGAT AAATTAGAGT ACATAGACCA GGAAAATAAA 
TCAGAAAATA CCACCTCTTT TGTTGGAACT CCTGAAAATA TACAATTTCA AAAAGAACCA 
AACTCTACTG TCTTCACAAA TATTAGTGCA CCCAACCAGT TAGACAGCTT TACGAAAACA 
AAAACTTCTG AAAAATGTAA ACAACTAAAC AATGATAAAC AGTCATCGGA ACCTAGTTCA 
GGTAGCCCTT GTGATAAAGA AAAGAGAAAA CATCTGTATA GACAAACAGC CACAGAATTA 
GATGCCTGTA TAGATACCAC ACTAGCTGAA AAGGTGAAGG GTGTGCAAAT TAATGAAAAA 
ATCACTGTCC CACATGTTTT GCATGCCAAG AAAACTACAC TAAAAGCACC TGTTAACCGT 
AGAATGCCTC ATGTTACAAG CACTAGCAAG CTTTCTCCAA CTAAAAGGAG CTTGTCTGAA 
ACAGTAACTC ACAGAGCCAA AATCATGAAA ATTGCTACTA AAAAGCGAAA TAGTGTTCAT 
GTTACTTTTA GACCTTCTAC TGAGTCAGTT CAGTTTTATA ATCCTCTGGA AAACAAAGAG 
GCTCCATGGA AGATGAGACT GCGGAAGCTC AGTGGCTTTA GTGGTAGTAA TAGCAACATC 
AGCAACCCCC ACACCAGCTC CAACAGTGCT GCAGAGCTAG TAAAACCTGG TGTGTACAGG 
CCTCTAGATA CAATTAGTGC AGCATCAGCA AGCAGTGAAA CAATTAAGGA AACGACAGAG 
ATTCCCACCG CCATATTACA GAAAGAAGGA ATTGCAACTA ATCAGTTAGG TAGTCGTAGT 
ACTATTAAGT CATCAAGTCA CGAGGCAGGA CTACCGCAGG GCTCCCTGGG TGGCGTTTAT 
AAAACTGTTG TCCATGCTCT TTCGAAGCCG AAGGCAAATG TTTCCCCACA GAGGCAGAAC 
AGAATGCCAC CAGAGGCTCC ACTGAGGGAT CTGTACAGTC ATGTAATGGG CTATTTTGGA 
AGGAAAGCTG CAGTCAATAA AGAGGACATG AGCACAAAAC TGCCTCCTCT TAATAGTGAT 
ATTGGCAGCA GCAATGCTAA TGTTCCTGAT CTGATGGATG AGTTTATAGC AGAACGACTT 
CGAAGTGGTA ATGCCTCAAC TATGACACGA AGAGGAAGTA GTCCAGGTAG CCTCGAAATT 
CCCAAAGACC TCCCTGATAT TCTAAATAAG CAAAACCAAA TGCGTCCTAT TGATGACCCA 
GGTGTACCCT CAGAATGGAC TTCTCCTGCC AGTGCAGGGA GCAGTGATCT TATCAGCTCA 
GATAGTCATT CGGATTCTTT CAGCGCTTTC CAGTATGATG GCCGAAAATT TGACAATTTT 
GGTTTTGGAG CTGATACTGG GAATGCATCC TCTGCTGACA TGGATTCAGG TTCTGGCCAT 
TATCAGAGTG CTGAAGAGCA AGAGGTGGCT AGTCTAACCA CACTACACAT TGACTCAGAA 
ACAAGCAGTC TTAATCAACA AGCTTTCTGT ACTGAAGTTG CAACGGTTAC TGGTTCAGAA 
AGTGCCTCTC CAGTCCATTC AGCTCTGGGA TCCAGGTCAC AGACACCTTC TCCTTCCACA 
CTGAATATAG ATCACATGGA ACAGAAGGAT CTACAGCTCG ATGAGAAGCT CCACCACTCT 
GTTCTTCAGA CACCAGATGA CCTAGAAATC AGTGAATTTC CATCTGAATG CTGTAGTGTG 
ATGGCAGGGG GCACTCTTAC AGGATGGCAT GCTGATGTTG CTACTGTAAT GTGGCGAAGG 
ATGCTGGGTA TTTTGGGAGA TGTCAACGCT ATTATGGATC CTGAAATACA TGCTCAAGTT 
TTTGATTACC TCTGTGAACT TTGGCAGAAT CTAGCTAAGA TTAGAGATAA TCTTGGCATT 
TCAACTGATA ACCTGACCTC CCCTTCTCCA CCGGTTTTAA TTCCTCCACT GAGAATTCTT 
ACACCTTGGC TTTTTAAGGC AACCATGTTA ACTGATAAAT ATAAACAAGG TAAATTACAT 
GCCTATAAAC TTATTTGTAA TACAATGAAA AGAAGACAAG ATGTTTCTCC AAATAGAGAT 
TTTCTAACAC ATTTCTACAA CATAATGCAT TGTGGATTGC TTCACATTGA TCAGGATATT 
GTCAATACAA TCATCAAGCA CTGCTCACCT CAATTTTTCT CACTTGGTTT GCCTGGTGCA 
ACAATGCTTA TTATGGATTT TATTGTAGCA GCTGGTAGAG TGGCTTCTTC AGCTTTTCTC 
AATGCACCAA GAGTGGAAGC ACAAGTTCTT CTGGGATCTT TGGTTTGCTT TCCCAACTTG 
TATTGTGAAC TGCCTGCTCT CCATCCCAAC ATCCCTGATA TTGCTGTGTC TCAATTTACA 
GATGTTAAGG AACTTATAAT CAAAACTGTG TTAAGCTCTG CAAGAGATGA GCCCTCTGGT 
CCTGCACGAT GTGTAGCACT TTGCAGTTTA GGTATTTGGA TTTGTGAAGA ACTAGTCCAT 
GAGTCTCATC ATCCTCAAAT TAAGGAAGCT CTGAATGTAA TTTGTGTTTC CTTAAAGTTT 
ACTAATAAAA CAGTAGCCCA TGTAGCTTGT AACATGCTTC ACATGCTGGT TCATTATGTA 
CCTAGACTTC AGATTTACCA GCCTGATTCT CCCTTGAAAA TTATTCAAAT TCTAATAGCC 
ACTATTACCC ATCTTTTGCC AAGTACGGAA GCGTCATCTT ATGAAATGGA CAAGAGGTTG 
GTGGTATCCT TACTTCTGTG TCTTCTGGAC TGGATCATGG CCTTACCTCT TAAGACACTG 
CTCCAACCAG TCCATGCTAC AGGAGCAGAA AATGATAAAA TAGAAAAATC TGTTCTTAAC 
TGCATTTATA AGGTATTACA TGGGTGTGTT TATGGAGCTC AGTGTTTTAG CAATCCGAGG 
TATTTTCCAA TAAGCCTTTC TGATTTGGCA TCTGTAGATT ATGATCCTTT TATGCATTTG 
GAAAGCCTAA AAGAGCCTGA ACCTTTGCAC TCTCCTGATT CGGAACGATC TTCTAAACTC 
CAGCCAGTAA CAGAAGTGAA AACTCAGATG CAGCAAGGAT TAATCTCCAT AGCTGCCCGC 
ACTGTTATCA CACATCTGGT GAATCACTTG GGCCATTATC CAATGAGCGG TGGTCCTGCT 
ATGTTAACAA GTCAGGTGTG TGAGAATCAC GACAATCATT ACAGTGAAAG CACTGAACTT 
TCCCCTGAAC TCTTCGAGAG TCCAAATATT CAGTTTTTTG TGTTAAACAA TACAACCTTA 
GTGTCCTGTA TCCAGATCAG AGCTGAAGAG AGTATACCTG GAGGAGGTTT ATCTGCTGGT 
CTTGCATCAG CCAATTCAAA TGTCAGAATT ATAGTACGTG ATCTTTCTGG AAAATATTCA 
TGGGATTCTG CCATCCTGTA TGGACCACCT CTTGTAAGTG GTCTGTCAGA ACCTACATCT 
CTCGTACTTT CATTGTCTCA CCAAGAGAAG CCAGAAGAAC CTTCTGCATC TAATGAATGC 
TTAGAAGATA TAACAGTTAA AGATGGGATT TCCCTCCAGT TTAAAAGAAG TTTTAGAGAA 
ACTGTACCAA CTTGGGACAC AATCAGAGAT GAAGAAGATG TTCTTGATGA GCTTTTGCAG 
TATTTAGGTG TAACCAGTCC TGAATGCTTA CAGAGAACTG GAGTCTCACT TAATATTCCT 
GCTCCACAGC CTGTGTGCAT TTCTGAAAAA CAGGAGAATG ATGTTATTAA TGCTATCCTT 
AAGCAGCATA CAGAGGAAAA AGAATTTGTT GAGAAACACT TTAATGACTT AAACATGAAA 
GCTGCAGAAC AAGATGAACC AACACCTCAG AAACCTCAGT CAGCATTTTA TTATTGCAGA 
TTGCTTCTTA GTATATTGGG AATGAATTCC TGGGACAAAA GGAGGAGCTT TCATCTCCTG 
AAGAAAAATG AAAAACTACT TAGAGAACTT AGGAACTTGG ACTCAAGACA GTGCCGAGAG 
ACACACAAGA TTGCAGTATT TTATGTTGCT GAAGGACAAG AAGACAAACA TTCCATTCTC 
ACCAATACAG GAGGAAGTCA AGCATATGAA GATTTTGTAG CTGGTCTTGG TTGGGAGGTA 
AATCTTACAA ACCATTGTGG TTTCATGGGA GGACTACAAA AAAATAAAAG CACTGGACTG 
ACCACACCGT ATTTTGCTAC CTCTACAGTT GAAGTAATAT TTCATGTGTC AACAAGAATG 
CCTTCAGATT CTGATGACTC TTTGACCAAA AAATTGAGAC ATTTAGGAAA TGATGAAGTG 
CACATTGTTT GGTCAGAGCA TACTAGAGAC TACAGGAGAG GAATTATTCC TACAGAATTT 
GGTGATGTCC TTATTGTTAT ATACCCGATG AAAAACCACA TGTTCAGTAT CCAGATAATG 
AAAAAACCAG AGGTTCCCTT CTTTGGTCCA CTCTTTGATG GTGCTATTGT GAATGGAAAG 
GTCCTACCTA TTATGGTTCG AGCAACAGCT ATAAATGCAA GCCGTGCTCT GAAATCCCTG 
ATTCCATTGT ATCAAAACTT CTATGAGGAG AGAGCACGAT ATCTGCAAAC GATTGTCCAG 
CACCATTTAG AGCCAACGAC ATTTGAAGAT TTTGCAGCAC AGGTTTTTTC TCCAGCTCCC 
TACCATCATT TACCATCTGA TGCCGTGGGC TCCTACCCAG AGATCCTATC CAGTGAAACT 
CCCACAGCGG CGCAGGTAGA CGGGGCCGAC CTGGCCTCTC CAATGTCTCC TCGAACGAGC 
AAAAGCCGCA TGTCCATGAA GCTGCGTCGC TCCTCTGGCT CCGCCAATAA GTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_012130796.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986186.1 Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X21, mRNA.

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CloneID	OOv14058
Clone ID Related Accession (Same CDS sequence)	XM_012130796.2 , XM_012130797.2
Accession Version	XM_012130796.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	ral GTPase-activating protein subunit alpha-1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942367.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012130796.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCGCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAATGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTGACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GCCATGAGAA GCCGATCCAT TGGTGAATGT 
GCTCTGCCAT CGGCCTATAT ACGCAGTGCT AAAAGTGCTC CTGTTCTGAT CCATACTTCC 
AAACCCTTCT TGCCTGATAT TGTTCTCACT CCCCTTTCTG ATGAGCTTTC AGATATTGAT 
GATGCTCAAA TACTTCCTCG TCCAACTAGA GTCAGACATT TTTCGCAAAG TGAAGACACT 
GGAAATGAAG TTTTTGGTGC TTTGAGTGAG GAGCAGCCAT TGCCTCGAAG TAGCAGCACT 
TCTGACATCT TGGAACCATT CACTGTTGAA CGAGCCAAAG GTGCAGTTCC TGTCATTGAC 
AGTTCATCCC GTCATGCACC AAGCTTGCAG AGTTCCACAG AGGCTTCTTC AATAACTAGA 
TCCACTGAGA GCCACATCAC TGATACTCAT AGTAGAGAGT CTTCTCTGGA AGTTGGTGAT 
AGCATATATG ACCATCTCTG TCACTTAATA GATCCAGTAG AACTTGCAGA TTCAGCTTTT 
GAACAAATCC AGTACATTGA CCTTGAAGGA GATGATGATC TTCTTTCCTC CCTGAAAGAA 
TATTTTAAGG AAACCCAAGA AAGTCACAGT AAAAATGAGT TAGATAAAGA TGCAGCTTCG 
CAGGAAGTGA TCTCTGTTGC AGTAAATAAG GATGAAAGAT CATCCTCAGA TAAATTAGAG 
TACATAGACC AGGAAAATAA ATCAGAAAAT ACCACCTCTT TTGTTGGAAC TCCTGAAAAT 
ATACAATTTC AAAAAGAACC AAACTCTACT GTCTTCACAA ATATTAGTGC ACCCAACCAG 
TTAGACAGCT TTACGAAAAC AAAAACTTCT GAAAAATGTA AACAACTAAA CAATGATAAA 
CAGTCATCGG AACCTAGTTC AGGTAGCCCT TGTGATAAAG AAAAGAGAAA ACATCTGTAT 
AGACAAACAG CCACAGAATT AGATGCCTGT ATAGATACCA CACTAGCTGA AAAGGTGAAG 
GGTGTGCAAA TTAATGAAAA AATCACTGTC CCACATGTTT TGCATGCCAA GAAAACTACA 
CTAAAAGCAC CTGTTAACCG TAGAATGCCT CATGTTACAA GCACTAGCAA GCTTTCTCCA 
ACTAAAAGGA GCTTGTCTGA AACAGTAACT CACAGAGCCA AAATCATGAA AATTGCTACT 
AAAAAGCGAA ATAGTGTTCA TGTTACTTTT AGACCTTCTA CTGAGTCAGT TCAGTTTTAT 
AATCCTCTGG AAAACAAAGA GGCTCCATGG AAGATGAGAC TGCGGAAGCT CAGTGGCTTT 
AGTGGTAGTA ATAGCAACAT CAGCAACCCC CACACCAGCT CCAACAGTGC TGCAGAGCTA 
GTAAAACCTG GTGTGTACAG GCCTCTAGAT ACAATTAGTG CAGCATCAGC AAGCAGTGAA 
ACAATTAAGG AAACGACAGA GATTCCCACC GCCATATTAC AGAAAGAAGG AATTGCAACT 
AATCAGTTAG GTAGTCGTAG TACTATTAAG TCATCAAGTC ACGAGGCAGG ACTACCGCAG 
GGCTCCCTGG GTGGCGTTTA TAAAACTGTT GTCCATGCTC TTTCGAAGCC GAAGGCAAAT 
GTTTCCCCAC AGAGGCAGAA CAGAATGCCA CCAGAGGCTC CACTGAGGGA TCTGTACAGT 
CATGTAATGG GCTATTTTGG AAGGAAAGCT GCAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAGCCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGATCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986186.1
CDS	457..8130
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIGRIFTAEPPEKPREHENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQENKSENTTSFVGTPENIQFQKEPNSTVFTNISAPNQLDSFTKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDTTLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDADH

Target ORF information:

RefSeq Version	XM_012130796.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130796.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCGCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAATGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTGACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GCCATGAGAA GCCGATCCAT TGGTGAATGT 
GCTCTGCCAT CGGCCTATAT ACGCAGTGCT AAAAGTGCTC CTGTTCTGAT CCATACTTCC 
AAACCCTTCT TGCCTGATAT TGTTCTCACT CCCCTTTCTG ATGAGCTTTC AGATATTGAT 
GATGCTCAAA TACTTCCTCG TCCAACTAGA GTCAGACATT TTTCGCAAAG TGAAGACACT 
GGAAATGAAG TTTTTGGTGC TTTGAGTGAG GAGCAGCCAT TGCCTCGAAG TAGCAGCACT 
TCTGACATCT TGGAACCATT CACTGTTGAA CGAGCCAAAG GTGCAGTTCC TGTCATTGAC 
AGTTCATCCC GTCATGCACC AAGCTTGCAG AGTTCCACAG AGGCTTCTTC AATAACTAGA 
TCCACTGAGA GCCACATCAC TGATACTCAT AGTAGAGAGT CTTCTCTGGA AGTTGGTGAT 
AGCATATATG ACCATCTCTG TCACTTAATA GATCCAGTAG AACTTGCAGA TTCAGCTTTT 
GAACAAATCC AGTACATTGA CCTTGAAGGA GATGATGATC TTCTTTCCTC CCTGAAAGAA 
TATTTTAAGG AAACCCAAGA AAGTCACAGT AAAAATGAGT TAGATAAAGA TGCAGCTTCG 
CAGGAAGTGA TCTCTGTTGC AGTAAATAAG GATGAAAGAT CATCCTCAGA TAAATTAGAG 
TACATAGACC AGGAAAATAA ATCAGAAAAT ACCACCTCTT TTGTTGGAAC TCCTGAAAAT 
ATACAATTTC AAAAAGAACC AAACTCTACT GTCTTCACAA ATATTAGTGC ACCCAACCAG 
TTAGACAGCT TTACGAAAAC AAAAACTTCT GAAAAATGTA AACAACTAAA CAATGATAAA 
CAGTCATCGG AACCTAGTTC AGGTAGCCCT TGTGATAAAG AAAAGAGAAA ACATCTGTAT 
AGACAAACAG CCACAGAATT AGATGCCTGT ATAGATACCA CACTAGCTGA AAAGGTGAAG 
GGTGTGCAAA TTAATGAAAA AATCACTGTC CCACATGTTT TGCATGCCAA GAAAACTACA 
CTAAAAGCAC CTGTTAACCG TAGAATGCCT CATGTTACAA GCACTAGCAA GCTTTCTCCA 
ACTAAAAGGA GCTTGTCTGA AACAGTAACT CACAGAGCCA AAATCATGAA AATTGCTACT 
AAAAAGCGAA ATAGTGTTCA TGTTACTTTT AGACCTTCTA CTGAGTCAGT TCAGTTTTAT 
AATCCTCTGG AAAACAAAGA GGCTCCATGG AAGATGAGAC TGCGGAAGCT CAGTGGCTTT 
AGTGGTAGTA ATAGCAACAT CAGCAACCCC CACACCAGCT CCAACAGTGC TGCAGAGCTA 
GTAAAACCTG GTGTGTACAG GCCTCTAGAT ACAATTAGTG CAGCATCAGC AAGCAGTGAA 
ACAATTAAGG AAACGACAGA GATTCCCACC GCCATATTAC AGAAAGAAGG AATTGCAACT 
AATCAGTTAG GTAGTCGTAG TACTATTAAG TCATCAAGTC ACGAGGCAGG ACTACCGCAG 
GGCTCCCTGG GTGGCGTTTA TAAAACTGTT GTCCATGCTC TTTCGAAGCC GAAGGCAAAT 
GTTTCCCCAC AGAGGCAGAA CAGAATGCCA CCAGAGGCTC CACTGAGGGA TCTGTACAGT 
CATGTAATGG GCTATTTTGG AAGGAAAGCT GCAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAGCCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGATCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_012130797.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986187.1 Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X22, mRNA.

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CloneID	OOv14058
Clone ID Related Accession (Same CDS sequence)	XM_012130796.2 , XM_012130797.2
Accession Version	XM_012130797.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	ral GTPase-activating protein subunit alpha-1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942367.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012130797.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCGCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAATGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTGACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GCCATGAGAA GCCGATCCAT TGGTGAATGT 
GCTCTGCCAT CGGCCTATAT ACGCAGTGCT AAAAGTGCTC CTGTTCTGAT CCATACTTCC 
AAACCCTTCT TGCCTGATAT TGTTCTCACT CCCCTTTCTG ATGAGCTTTC AGATATTGAT 
GATGCTCAAA TACTTCCTCG TCCAACTAGA GTCAGACATT TTTCGCAAAG TGAAGACACT 
GGAAATGAAG TTTTTGGTGC TTTGAGTGAG GAGCAGCCAT TGCCTCGAAG TAGCAGCACT 
TCTGACATCT TGGAACCATT CACTGTTGAA CGAGCCAAAG GTGCAGTTCC TGTCATTGAC 
AGTTCATCCC GTCATGCACC AAGCTTGCAG AGTTCCACAG AGGCTTCTTC AATAACTAGA 
TCCACTGAGA GCCACATCAC TGATACTCAT AGTAGAGAGT CTTCTCTGGA AGTTGGTGAT 
AGCATATATG ACCATCTCTG TCACTTAATA GATCCAGTAG AACTTGCAGA TTCAGCTTTT 
GAACAAATCC AGTACATTGA CCTTGAAGGA GATGATGATC TTCTTTCCTC CCTGAAAGAA 
TATTTTAAGG AAACCCAAGA AAGTCACAGT AAAAATGAGT TAGATAAAGA TGCAGCTTCG 
CAGGAAGTGA TCTCTGTTGC AGTAAATAAG GATGAAAGAT CATCCTCAGA TAAATTAGAG 
TACATAGACC AGGAAAATAA ATCAGAAAAT ACCACCTCTT TTGTTGGAAC TCCTGAAAAT 
ATACAATTTC AAAAAGAACC AAACTCTACT GTCTTCACAA ATATTAGTGC ACCCAACCAG 
TTAGACAGCT TTACGAAAAC AAAAACTTCT GAAAAATGTA AACAACTAAA CAATGATAAA 
CAGTCATCGG AACCTAGTTC AGGTAGCCCT TGTGATAAAG AAAAGAGAAA ACATCTGTAT 
AGACAAACAG CCACAGAATT AGATGCCTGT ATAGATACCA CACTAGCTGA AAAGGTGAAG 
GGTGTGCAAA TTAATGAAAA AATCACTGTC CCACATGTTT TGCATGCCAA GAAAACTACA 
CTAAAAGCAC CTGTTAACCG TAGAATGCCT CATGTTACAA GCACTAGCAA GCTTTCTCCA 
ACTAAAAGGA GCTTGTCTGA AACAGTAACT CACAGAGCCA AAATCATGAA AATTGCTACT 
AAAAAGCGAA ATAGTGTTCA TGTTACTTTT AGACCTTCTA CTGAGTCAGT TCAGTTTTAT 
AATCCTCTGG AAAACAAAGA GGCTCCATGG AAGATGAGAC TGCGGAAGCT CAGTGGCTTT 
AGTGGTAGTA ATAGCAACAT CAGCAACCCC CACACCAGCT CCAACAGTGC TGCAGAGCTA 
GTAAAACCTG GTGTGTACAG GCCTCTAGAT ACAATTAGTG CAGCATCAGC AAGCAGTGAA 
ACAATTAAGG AAACGACAGA GATTCCCACC GCCATATTAC AGAAAGAAGG AATTGCAACT 
AATCAGTTAG GTAGTCGTAG TACTATTAAG TCATCAAGTC ACGAGGCAGG ACTACCGCAG 
GGCTCCCTGG GTGGCGTTTA TAAAACTGTT GTCCATGCTC TTTCGAAGCC GAAGGCAAAT 
GTTTCCCCAC AGAGGCAGAA CAGAATGCCA CCAGAGGCTC CACTGAGGGA TCTGTACAGT 
CATGTAATGG GCTATTTTGG AAGGAAAGCT GCAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAGCCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGATCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986187.1
CDS	457..8130
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIGRIFTAEPPEKPREHENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQENKSENTTSFVGTPENIQFQKEPNSTVFTNISAPNQLDSFTKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDTTLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDADH

Target ORF information:

RefSeq Version	XM_012130797.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130797.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCGCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAATGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTGACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GCCATGAGAA GCCGATCCAT TGGTGAATGT 
GCTCTGCCAT CGGCCTATAT ACGCAGTGCT AAAAGTGCTC CTGTTCTGAT CCATACTTCC 
AAACCCTTCT TGCCTGATAT TGTTCTCACT CCCCTTTCTG ATGAGCTTTC AGATATTGAT 
GATGCTCAAA TACTTCCTCG TCCAACTAGA GTCAGACATT TTTCGCAAAG TGAAGACACT 
GGAAATGAAG TTTTTGGTGC TTTGAGTGAG GAGCAGCCAT TGCCTCGAAG TAGCAGCACT 
TCTGACATCT TGGAACCATT CACTGTTGAA CGAGCCAAAG GTGCAGTTCC TGTCATTGAC 
AGTTCATCCC GTCATGCACC AAGCTTGCAG AGTTCCACAG AGGCTTCTTC AATAACTAGA 
TCCACTGAGA GCCACATCAC TGATACTCAT AGTAGAGAGT CTTCTCTGGA AGTTGGTGAT 
AGCATATATG ACCATCTCTG TCACTTAATA GATCCAGTAG AACTTGCAGA TTCAGCTTTT 
GAACAAATCC AGTACATTGA CCTTGAAGGA GATGATGATC TTCTTTCCTC CCTGAAAGAA 
TATTTTAAGG AAACCCAAGA AAGTCACAGT AAAAATGAGT TAGATAAAGA TGCAGCTTCG 
CAGGAAGTGA TCTCTGTTGC AGTAAATAAG GATGAAAGAT CATCCTCAGA TAAATTAGAG 
TACATAGACC AGGAAAATAA ATCAGAAAAT ACCACCTCTT TTGTTGGAAC TCCTGAAAAT 
ATACAATTTC AAAAAGAACC AAACTCTACT GTCTTCACAA ATATTAGTGC ACCCAACCAG 
TTAGACAGCT TTACGAAAAC AAAAACTTCT GAAAAATGTA AACAACTAAA CAATGATAAA 
CAGTCATCGG AACCTAGTTC AGGTAGCCCT TGTGATAAAG AAAAGAGAAA ACATCTGTAT 
AGACAAACAG CCACAGAATT AGATGCCTGT ATAGATACCA CACTAGCTGA AAAGGTGAAG 
GGTGTGCAAA TTAATGAAAA AATCACTGTC CCACATGTTT TGCATGCCAA GAAAACTACA 
CTAAAAGCAC CTGTTAACCG TAGAATGCCT CATGTTACAA GCACTAGCAA GCTTTCTCCA 
ACTAAAAGGA GCTTGTCTGA AACAGTAACT CACAGAGCCA AAATCATGAA AATTGCTACT 
AAAAAGCGAA ATAGTGTTCA TGTTACTTTT AGACCTTCTA CTGAGTCAGT TCAGTTTTAT 
AATCCTCTGG AAAACAAAGA GGCTCCATGG AAGATGAGAC TGCGGAAGCT CAGTGGCTTT 
AGTGGTAGTA ATAGCAACAT CAGCAACCCC CACACCAGCT CCAACAGTGC TGCAGAGCTA 
GTAAAACCTG GTGTGTACAG GCCTCTAGAT ACAATTAGTG CAGCATCAGC AAGCAGTGAA 
ACAATTAAGG AAACGACAGA GATTCCCACC GCCATATTAC AGAAAGAAGG AATTGCAACT 
AATCAGTTAG GTAGTCGTAG TACTATTAAG TCATCAAGTC ACGAGGCAGG ACTACCGCAG 
GGCTCCCTGG GTGGCGTTTA TAAAACTGTT GTCCATGCTC TTTCGAAGCC GAAGGCAAAT 
GTTTCCCCAC AGAGGCAGAA CAGAATGCCA CCAGAGGCTC CACTGAGGGA TCTGTACAGT 
CATGTAATGG GCTATTTTGG AAGGAAAGCT GCAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAGCCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGATCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_012130798.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986188.1 Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X23, mRNA.

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CloneID	OOv14059
Clone ID Related Accession (Same CDS sequence)	XM_012130798.2
Accession Version	XM_012130798.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	ral GTPase-activating protein subunit alpha-1 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942367.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012130798.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCGCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAATGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTGACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GCCATGAGAA GCCGATCCAT TGGTGAATGT 
GCTCTGCCAT CGGCCTATAT ACGCAGTGCT AAAAGTGCTC CTGTTCTGAT CCATACTTCC 
AAACCCTTCT TGCCTGATAT TGTTCTCACT CCCCTTTCTG ATGAGCTTTC AGATATTGAT 
GATGCTCAAA TACTTCCTCG TCCAACTAGA GTCAGACATT TTTCGCAAAG TGAAGACACT 
GGAAATGAAG TTTTTGGTGC TTTGAGTGAG GAGCAGCCAT TGCCTCGAAG TAGCAGCACT 
TCTGACATCT TGGAACCATT CACTGTTGAA CGAGCCAAAG GTGCAGTTCC TGTCATTGAC 
AGTTCATCCC GTCATGCACC AAGCTTGCAG AGTTCCACAG AGGCTTCTTC AATAACTAGA 
TCCACTGAGA GCCACATCAC TGATACTCAT AGTAGAGAGT CTTCTCTGGA AGTTGGTGAT 
AGCATATATG ACCATCTCTG TCACTTAATA GATCCAGTAG AACTTGCAGA TTCAGCTTTT 
GAACAAATCC AGTACATTGA CCTTGAAGGA GATGATGATC TTCTTTCCTC CCTGAAAGAA 
TATTTTAAGG AAACCCAAGA AAGTCACAGT AAAAATGAGT TAGATAAAGA TGCAGCTTCG 
CAGGAAGTGA TCTCTGTTGC AGTAAATAAG GATGAAAGAT CATCCTCAGA TAAATTAGAG 
TACATAGACC AGGAAAATAA ATCAGAAAAT ACCACCTCTT TTGTTGGAAC TCCTGAAAAT 
ATACAATTTC AAAAAGAACC AAACTCTACT GTCTTCACAA ATATTAGTGC ACCCAACCAG 
TTAGACAGCT TTACGAAAAC AAAAACTTCT GAAAAATGTA AACAACTAAA CAATGATAAA 
CAGTCATCGG AACCTAGTTC AGGTAGCCCT TGTGATAAAG AAAAGAGAAA ACATCTGTAT 
AGACAAACAG CCACAGAATT AGATGCCTGT ATAGATACCA CACTAGCTGA AAAGGTGAAG 
GGTGTGCAAA TTAATGAAAA AATCACTGTC CCACATGTTT TGCATGCCAA GAAAACTACA 
CTAAAAGCAC CTGTTAACCG TAGAATGCCT CATGTTACAA GCACTAGCAA GCTTTCTCCA 
ACTAAAAGGA GCTTGTCTGA AACAGTAACT CACAGAGCCA AAATCATGAA AATTGCTACT 
AAAAAGCGAA ATAGTGTTCA TGTTACTTTT AGACCTTCTA CTGAGTCAGT TCAGTTTTAT 
AATCCTCTGG AAAACAAAGA GGCTCCATGG AAGATGAGAC TGCGGAAGCT CAGTGGCTTT 
AGTGGTAGTA ATAGCAACAT CAGCAACCCC CACACCAGCT CCAACAGTGC TGCAGAGCTA 
GTAAAACCTG GTGTGTACAG GCCTCTAGAT ACAATTAGTG CAGCATCAGC AAGCAGTGAA 
ACAATTAAGG AAACGACAGA GATTCCCACC GCCATATTAC AGAAAGAAGG AATTGCAACT 
AATCAGTTAG GTAGTCGTAG TACTATTAAG TCATCAAGTC ACGAGGCAGG ACTACCGCAG 
GGCTCCCTGG GTGGCGTTTA TAAAACTGTT GTCCATGCTC TTTCGAAGCC GAAGGCAAAT 
GTTTCCCCAC AGAGGCAGAA CAGAATGCCA CCAGAGGCTC CACTGAGGGA TCTGTACAGT 
CATGTAATGG GCTATTTTGG AAGGAAAGCT GCAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TGATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986188.1
CDS	457..8004
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIGRIFTAEPPEKPREHENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQENKSENTTSFVGTPENIQFQKEPNSTVFTNISAPNQLDSFTKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDTTLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNLI

Target ORF information:

RefSeq Version	XM_012130798.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130798.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCGCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAATGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTGACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GCCATGAGAA GCCGATCCAT TGGTGAATGT 
GCTCTGCCAT CGGCCTATAT ACGCAGTGCT AAAAGTGCTC CTGTTCTGAT CCATACTTCC 
AAACCCTTCT TGCCTGATAT TGTTCTCACT CCCCTTTCTG ATGAGCTTTC AGATATTGAT 
GATGCTCAAA TACTTCCTCG TCCAACTAGA GTCAGACATT TTTCGCAAAG TGAAGACACT 
GGAAATGAAG TTTTTGGTGC TTTGAGTGAG GAGCAGCCAT TGCCTCGAAG TAGCAGCACT 
TCTGACATCT TGGAACCATT CACTGTTGAA CGAGCCAAAG GTGCAGTTCC TGTCATTGAC 
AGTTCATCCC GTCATGCACC AAGCTTGCAG AGTTCCACAG AGGCTTCTTC AATAACTAGA 
TCCACTGAGA GCCACATCAC TGATACTCAT AGTAGAGAGT CTTCTCTGGA AGTTGGTGAT 
AGCATATATG ACCATCTCTG TCACTTAATA GATCCAGTAG AACTTGCAGA TTCAGCTTTT 
GAACAAATCC AGTACATTGA CCTTGAAGGA GATGATGATC TTCTTTCCTC CCTGAAAGAA 
TATTTTAAGG AAACCCAAGA AAGTCACAGT AAAAATGAGT TAGATAAAGA TGCAGCTTCG 
CAGGAAGTGA TCTCTGTTGC AGTAAATAAG GATGAAAGAT CATCCTCAGA TAAATTAGAG 
TACATAGACC AGGAAAATAA ATCAGAAAAT ACCACCTCTT TTGTTGGAAC TCCTGAAAAT 
ATACAATTTC AAAAAGAACC AAACTCTACT GTCTTCACAA ATATTAGTGC ACCCAACCAG 
TTAGACAGCT TTACGAAAAC AAAAACTTCT GAAAAATGTA AACAACTAAA CAATGATAAA 
CAGTCATCGG AACCTAGTTC AGGTAGCCCT TGTGATAAAG AAAAGAGAAA ACATCTGTAT 
AGACAAACAG CCACAGAATT AGATGCCTGT ATAGATACCA CACTAGCTGA AAAGGTGAAG 
GGTGTGCAAA TTAATGAAAA AATCACTGTC CCACATGTTT TGCATGCCAA GAAAACTACA 
CTAAAAGCAC CTGTTAACCG TAGAATGCCT CATGTTACAA GCACTAGCAA GCTTTCTCCA 
ACTAAAAGGA GCTTGTCTGA AACAGTAACT CACAGAGCCA AAATCATGAA AATTGCTACT 
AAAAAGCGAA ATAGTGTTCA TGTTACTTTT AGACCTTCTA CTGAGTCAGT TCAGTTTTAT 
AATCCTCTGG AAAACAAAGA GGCTCCATGG AAGATGAGAC TGCGGAAGCT CAGTGGCTTT 
AGTGGTAGTA ATAGCAACAT CAGCAACCCC CACACCAGCT CCAACAGTGC TGCAGAGCTA 
GTAAAACCTG GTGTGTACAG GCCTCTAGAT ACAATTAGTG CAGCATCAGC AAGCAGTGAA 
ACAATTAAGG AAACGACAGA GATTCCCACC GCCATATTAC AGAAAGAAGG AATTGCAACT 
AATCAGTTAG GTAGTCGTAG TACTATTAAG TCATCAAGTC ACGAGGCAGG ACTACCGCAG 
GGCTCCCTGG GTGGCGTTTA TAAAACTGTT GTCCATGCTC TTTCGAAGCC GAAGGCAAAT 
GTTTCCCCAC AGAGGCAGAA CAGAATGCCA CCAGAGGCTC CACTGAGGGA TCTGTACAGT 
CATGTAATGG GCTATTTTGG AAGGAAAGCT GCAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TGATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_012130799.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986189.1 Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X24, mRNA.

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CloneID	OOv14060
Clone ID Related Accession (Same CDS sequence)	XM_012130799.2
Accession Version	XM_012130799.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	ral GTPase-activating protein subunit alpha-1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942367.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012130799.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCGCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAATGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTGACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GCCATGAGAA GCCGATCCAT TGGTGAATGT 
GCTCTGCCAT CGGCCTATAT ACGCAGTGCT AAAAGTGCTC CTGTTCTGAT CCATACTTCC 
AAACCCTTCT TGCCTGATAT TGTTCTCACT CCCCTTTCTG ATGAGCTTTC AGATATTGAT 
GATGCTCAAA TACTTCCTCG TCCAACTAGA GTCAGACATT TTTCGCAAAG TGAAGACACT 
GGAAATGAAG TTTTTGGTGC TTTGAGTGAG GAGCAGCCAT TGCCTCGAAG TAGCAGCACT 
TCTGACATCT TGGAACCATT CACTGTTGAA CGAGCCAAAG GTGCAGTTCC TGTCATTGAC 
AGTTCATCCC GTCATGCACC AAGCTTGCAG AGTTCCACAG AGGCTTCTTC AATAACTAGA 
TCCACTGAGA GCCACATCAC TGATACTCAT AGTAGAGAGT CTTCTCTGGA AGTTGGTGAT 
AGCATATATG ACCATCTCTG TCACTTAATA GATCCAGTAG AACTTGCAGA TTCAGCTTTT 
GAACAAATCC AGTACATTGA CCTTGAAGGA GATGATGATC TTCTTTCCTC CCTGAAAGAA 
TATTTTAAGG AAACCCAAGA AAGTCACAGT AAAAATGAGT TAGATAAAGA TGCAGCTTCG 
CAGGAAGTGA TCTCTGTTGC AGTAAATAAG GATGAAAGAT CATCCTCAGA TAAATTAGAG 
TACATAGACC AGGAAAATAA ATCAGAAAAT ACCACCTCTT TTGTTGGAAC TCCTGAAAAT 
ATACAATTTC AAAAAGAACC AAACTCTACT GTCTTCACAA ATATTAGTGC ACCCAACCAG 
TTAGACAGCT TTACGAAAAC AAAAACTTCT GAAAAATGTA AACAACTAAA CAATGATAAA 
CAGTCATCGG AACCTAGTTC AGGTAGCCCT TGTGATAAAG AAAAGAGAAA ACATCTGTAT 
AGACAAACAG CCACAGAATT AGATGCCTGT ATAGATACCA CACTAGCTGA AAAGGTGAAG 
GGTGTGCAAA TTAATGAAAA AATCACTGTC CCACATGTTT TGCATGCCAA GAAAACTACA 
CTAAAAGCAC CTGTTAACCG TAGAATGCCT CATGTTACAA GCACTAGCAA GCTTTCTCCA 
ACTAAAAGGA GCTTGTCTGA AACAGTAACT CACAGAGCCA AAATCATGAA AATTGCTACT 
AAAAAGCGAA ATAGTGTTCA TGTTACTTTT AGACCTTCTA CTGAGTCAGT TCAGTTTTAT 
AATCCTCTGG AAAACAAAGA GGCTCCATGG AAGATGAGAC TGCGGAAGCT CAGTGGCTTT 
AGTGGTAGTA ATAGCAACAT CAGCAACCCC CACACCAGCT CCAACAGTGC TGCAGAGCTA 
GTAAAACCTG GTGTGTACAG GCCTCTAGAT ACAATTAGTG CAGCATCAGC AAGCAGTGAA 
ACAATTAAGG AAACGACAGA GATTCCCACC GCCATATTAC AGAAAGAAGG AATTGCAACT 
AATCAGTTAG GTAGTCGTAG TACTATTAAG TCATCAAGTC ACGAGGCAGG ACTACCGCAG 
GGCTCCCTGG GTGGCGTTTA TAAAACTGTT GTCCATGCTC TTTCGAAGCC GAAGGCAAAT 
GTTTCCCCAC AGAGGCAGAA CAGAATGCCA CCAGAGGCTC CACTGAGGGA TCTGTACAGT 
CATGTAATGG GCTATTTTGG AAGGAAAGCT GCAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAAGTAATT 
GAGACATTTA GGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986189.1
CDS	457..7734
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIGRIFTAEPPEKPREHENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQENKSENTTSFVGTPENIQFQKEPNSTVFTNISAPNQLDSFTKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDTTLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKVIETFRK

Target ORF information:

RefSeq Version	XM_012130799.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130799.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCGCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAATGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTGACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GCCATGAGAA GCCGATCCAT TGGTGAATGT 
GCTCTGCCAT CGGCCTATAT ACGCAGTGCT AAAAGTGCTC CTGTTCTGAT CCATACTTCC 
AAACCCTTCT TGCCTGATAT TGTTCTCACT CCCCTTTCTG ATGAGCTTTC AGATATTGAT 
GATGCTCAAA TACTTCCTCG TCCAACTAGA GTCAGACATT TTTCGCAAAG TGAAGACACT 
GGAAATGAAG TTTTTGGTGC TTTGAGTGAG GAGCAGCCAT TGCCTCGAAG TAGCAGCACT 
TCTGACATCT TGGAACCATT CACTGTTGAA CGAGCCAAAG GTGCAGTTCC TGTCATTGAC 
AGTTCATCCC GTCATGCACC AAGCTTGCAG AGTTCCACAG AGGCTTCTTC AATAACTAGA 
TCCACTGAGA GCCACATCAC TGATACTCAT AGTAGAGAGT CTTCTCTGGA AGTTGGTGAT 
AGCATATATG ACCATCTCTG TCACTTAATA GATCCAGTAG AACTTGCAGA TTCAGCTTTT 
GAACAAATCC AGTACATTGA CCTTGAAGGA GATGATGATC TTCTTTCCTC CCTGAAAGAA 
TATTTTAAGG AAACCCAAGA AAGTCACAGT AAAAATGAGT TAGATAAAGA TGCAGCTTCG 
CAGGAAGTGA TCTCTGTTGC AGTAAATAAG GATGAAAGAT CATCCTCAGA TAAATTAGAG 
TACATAGACC AGGAAAATAA ATCAGAAAAT ACCACCTCTT TTGTTGGAAC TCCTGAAAAT 
ATACAATTTC AAAAAGAACC AAACTCTACT GTCTTCACAA ATATTAGTGC ACCCAACCAG 
TTAGACAGCT TTACGAAAAC AAAAACTTCT GAAAAATGTA AACAACTAAA CAATGATAAA 
CAGTCATCGG AACCTAGTTC AGGTAGCCCT TGTGATAAAG AAAAGAGAAA ACATCTGTAT 
AGACAAACAG CCACAGAATT AGATGCCTGT ATAGATACCA CACTAGCTGA AAAGGTGAAG 
GGTGTGCAAA TTAATGAAAA AATCACTGTC CCACATGTTT TGCATGCCAA GAAAACTACA 
CTAAAAGCAC CTGTTAACCG TAGAATGCCT CATGTTACAA GCACTAGCAA GCTTTCTCCA 
ACTAAAAGGA GCTTGTCTGA AACAGTAACT CACAGAGCCA AAATCATGAA AATTGCTACT 
AAAAAGCGAA ATAGTGTTCA TGTTACTTTT AGACCTTCTA CTGAGTCAGT TCAGTTTTAT 
AATCCTCTGG AAAACAAAGA GGCTCCATGG AAGATGAGAC TGCGGAAGCT CAGTGGCTTT 
AGTGGTAGTA ATAGCAACAT CAGCAACCCC CACACCAGCT CCAACAGTGC TGCAGAGCTA 
GTAAAACCTG GTGTGTACAG GCCTCTAGAT ACAATTAGTG CAGCATCAGC AAGCAGTGAA 
ACAATTAAGG AAACGACAGA GATTCCCACC GCCATATTAC AGAAAGAAGG AATTGCAACT 
AATCAGTTAG GTAGTCGTAG TACTATTAAG TCATCAAGTC ACGAGGCAGG ACTACCGCAG 
GGCTCCCTGG GTGGCGTTTA TAAAACTGTT GTCCATGCTC TTTCGAAGCC GAAGGCAAAT 
GTTTCCCCAC AGAGGCAGAA CAGAATGCCA CCAGAGGCTC CACTGAGGGA TCTGTACAGT 
CATGTAATGG GCTATTTTGG AAGGAAAGCT GCAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAAGTAATT 
GAGACATTTA GGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_012130800.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986190.1 Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X1, mRNA.

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CloneID	OOv14061
Clone ID Related Accession (Same CDS sequence)	XM_012130800.1
Accession Version	XM_012130800.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	ral GTPase-activating protein subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942367.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAAG TGGTAAAAGT ATATCAAGAA TGGATCCAGC AAGAGGAAAA ACCTTTGTTC 
ATGCAAGAGC CTGAAGAAAT TGTGATCTCT TCTTCAGACC TTCCGTGCAT TGACAATGTC 
ACAGACCATG ATATTTCAAT GGAAGAAGGA GAAAAAAGAG AAGAGGAAAA TGGGACCAAT 
GTTGCTGATC ATGTTCGAAA TTCCACTTGG CCTAAAAATG GAACCTACCA AGATATTCTT 
CATAATGCTT CTGAAGAAGC CATAGAACAA AACATACGAG CTGGTGCCCA GGCAGTTTTG 
CAGGTGTTTA TTATAAATTC TTCAAACATA TTTCTTCTTG AACCTGCAAA TGAGATAAAA 
AGTCTTCTGG ATGAGCACAC AGATATGTGT AAACGAATTC TTAACATTTA TCGGTTCATG 
GTTGTGCAAG TATCAATGGA CAAAAAGACT TGGGAACAGA TGCTGCTTGT GTTGCTCAGA 
GTCACGGAGT CTGTACTGAA GATGCCATCA CAAGCTTTTC TACAGTTCCA GGGGAAAAAA 
AACATGACTT TGGCAGGTCG ACTTGCAGGA CCACTTTTCC AGACTCTTAT AGTTGCCTGG 
ATTAAAGCAA ATCTAAATGT GTACATCTCT CGAGAACTTT GGGATGACTT ACTCTCAGTA 
TTGTCATCGT TGACCTATTG GGAAGAGTTA GCCACTGAAT GGTCGCTGAC TATGGAGACA 
CTAACTAAAG TTTTAGCTAG AAATTTGTAT AGTTTGGATC TCAGTGATTT ACCATTGGAT 
AAGCTGAGTG AACAGAAACA AAAAAAGCAC AAAGGGAAAG GAGTGGGACA TGAATTTCAG 
AAAGTTTCAG TTGACAAGTC ATTTTCTAGA GGATGGAGTC GCGATCAGCC TGGCCAAGCC 
CCAATGAGGC AGAGAAGTGC AACAACCACT GGTTCCCCAG GCACAGAAAA GGCAAGGAGT 
ATAGTACGGC AAAAAACTGT TGCCATGAGA AGCCGATCCA TTGGTGAATG TGCTCTGCCA 
TCGGCCTATA TACGCAGTGC TAAAAGTGCT CCTGTTCTGA TCCATACTTC CAAACCCTTC 
TTGCCTGATA TTGTTCTCAC TCCCCTTTCT GATGAGCTTT CAGATATTGA TGATGCTCAA 
ATACTTCCTC GTCCAACTAG AGTCAGACAT TTTTCGCAAA GTGAAGACAC TGGAAATGAA 
GTTTTTGGTG CTTTGAGTGA GGAGCAGCCA TTGCCTCGAA GTAGCAGCAC TTCTGACATC 
TTGGAACCAT TCACTGTTGA ACGAGCCAAA GGTGCAGTTC CTGTCATTGA CAGTTCATCC 
CGTCATGCAC CAAGCTTGCA GAGTTCCACA GAGGCTTCTT CAATAACTAG ATCCACTGAG 
AGCCACATCA CTGATACTCA TAGTAGAGAG TCTTCTCTGG AAGTTGGTGA TAGCATATAT 
GACCATCTCT GTCACTTAAT AGATCCAGTA GAACTTGCAG ATTCAGCTTT TGAACAAATC 
CAGTACATTG ACCTTGAAGG AGATGATGAT CTTCTTTCCT CCCTGAAAGA ATATTTTAAG 
GAAACCCAAG AAAGTCACAG TAAAAATGAG TTAGATAAAG ATGCAGCTTC GCAGGAAGTG 
ATCTCTGTTG CAGTAAATAA GGATGAAAGA TCATCCTCAG ATAAATTAGA GTACATAGAC 
CAGGAAAATA AATCAGAAAA TACCACCTCT TTTGTTGGAA CTCCTGAAAA TATACAATTT 
CAAAAAGAAC CAAACTCTAC TGTCTTCACA AATATTAGTG CACCCAACCA GTTAGACAGC 
TTTACGAAAA CAAAAACTTC TGAAAAATGT AAACAACTAA ACAATGATAA ACAGTCATCG 
GAACCTAGTT CAGGTAGCCC TTGTGATAAA GAAAAGAGAA AACATCTGTA TAGACAAACA 
GCCACAGAAT TAGATGCCTG TATAGATACC ACACTAGCTG AAAAGGTGAA GGGTGTGCAA 
ATTAATGAAA AAATCACTGT CCCACATGTT TTGCATGCCA AGAAAACTAC ACTAAAAGCA 
CCTGTTAACC GTAGAATGCC TCATGTTACA AGCACTAGCA AGCTTTCTCC AACTAAAAGG 
AGCTTGTCTG AAACAGTAAC TCACAGAGCC AAAATCATGA AAATTGCTAC TAAAAAGCGA 
AATAGTGTTC ATGTTACTTT TAGACCTTCT ACTGAGTCAG TTCAGTTTTA TAATCCTCTG 
GAAAACAAAG AGGCTCCATG GAAGATGAGA CTGCGGAAGC TCAGTGGCTT TAGTGGTAGT 
AATAGCAACA TCAGCAACCC CCACACCAGC TCCAACAGTG CTGCAGAGCT AGTAAAACCT 
GGTGTGTACA GGCCTCTAGA TACAATTAGT GCAGCATCAG CAAGCAGTGA AACAATTAAG 
GAAACGACAG AGATTCCCAC CGCCATATTA CAGAAAGAAG GAATTGCAAC TAATCAGTTA 
GGTAGTCGTA GTACTATTAA GTCATCAAGT CACGAGGCAG GACTACCGCA GGGCTCCCTG 
GGTGGCGTTT ATAAAACTGT TGTCCATGCT CTTTCGAAGC CGAAGGCAAA TGTTTCCCCA 
CAGAGGCAGA ACAGAATGCC ACCAGAGGCT CCACTGAGGG ATCTGTACAG TCATGTAATG 
GGCTATTTTG GAAGGAAAGC TGCAGTCAAT AAAGAGGACA TGAGCACAAA ACTGCCTCCT 
CTTAATAGTG ATATTGGCAG CAGCAATGCT AATGTTCCTG ATCTGATGGA TGAGTTTATA 
GCAGAACGAC TTCGAAGTGG TAATGCCTCA ACTATGACAC GAAGAGGAAG TAGTCCAGGT 
AGCCTCGAAA TTCCCAAAGA CCTCCCTGAT ATTCTAAATA AGCAAAACCA AATGCGTCCT 
ATTGATGACC CAGGTGTACC CTCAGAATGG ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT 
CTTATCAGCT CAGATAGTCA TTCGGATTCT TTCAGCGCTT TCCAGTATGA TGGCCGAAAA 
TTTGACAATT TTGGTTTTGG AGCTGATACT GGGAATGCAT CCTCTGCTGA CATGGATTCA 
GGTTCTGGCC ATTATCAGAG TGCTGAAGAG CAAGAGGTGG CTAGTCTAAC CACACTACAC 
ATTGACTCAG AAACAAGCAG TCTTAATCAA CAAGCTTTCT GTACTGAAGT TGCAACGGTT 
ACTGGTTCAG AAAGTGCCTC TCCAGTCCAT TCAGCTCTGG GATCCAGGTC ACAGACACCT 
TCTCCTTCCA CACTGAATAT AGATCACATG GAACAGAAGG ATCTACAGCT CGATGAGAAG 
CTCCACCACT CTGTTCTTCA GACACCAGAT GACCTAGAAA TCAGTGAATT TCCATCTGAA 
TGCTGTAGTG TGATGGCAGG GGGCACTCTT ACAGGATGGC ATGCTGATGT TGCTACTGTA 
ATGTGGCGAA GGATGCTGGG TATTTTGGGA GATGTCAACG CTATTATGGA TCCTGAAATA 
CATGCTCAAG TTTTTGATTA CCTCTGTGAA CTTTGGCAGA ATCTAGCTAA GATTAGAGAT 
AATCTTGGCA TTTCAACTGA TAACCTGACC TCCCCTTCTC CACCGGTTTT AATTCCTCCA 
CTGAGAATTC TTACACCTTG GCTTTTTAAG GCAACCATGT TAACTGATAA ATATAAACAA 
GGTAAATTAC ATGCCTATAA ACTTATTTGT AATACAATGA AAAGAAGACA AGATGTTTCT 
CCAAATAGAG ATTTTCTAAC ACATTTCTAC AACATAATGC ATTGTGGATT GCTTCACATT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCAC CTCAATTTTT CTCACTTGGT 
TTGCCTGGTG CAACAATGCT TATTATGGAT TTTATTGTAG CAGCTGGTAG AGTGGCTTCT 
TCAGCTTTTC TCAATGCACC AAGAGTGGAA GCACAAGTTC TTCTGGGATC TTTGGTTTGC 
TTTCCCAACT TGTATTGTGA ACTGCCTGCT CTCCATCCCA ACATCCCTGA TATTGCTGTG 
TCTCAATTTA CAGATGTTAA GGAACTTATA ATCAAAACTG TGTTAAGCTC TGCAAGAGAT 
GAGCCCTCTG GTCCTGCACG ATGTGTAGCA CTTTGCAGTT TAGGTATTTG GATTTGTGAA 
GAACTAGTCC ATGAGTCTCA TCATCCTCAA ATTAAGGAAG CTCTGAATGT AATTTGTGTT 
TCCTTAAAGT TTACTAATAA AACAGTAGCC CATGTAGCTT GTAACATGCT TCACATGCTG 
GTTCATTATG TACCTAGACT TCAGATTTAC CAGCCTGATT CTCCCTTGAA AATTATTCAA 
ATTCTAATAG CCACTATTAC CCATCTTTTG CCAAGTACGG AAGCGTCATC TTATGAAATG 
GACAAGAGGT TGGTGGTATC CTTACTTCTG TGTCTTCTGG ACTGGATCAT GGCCTTACCT 
CTTAAGACAC TGCTCCAACC AGTCCATGCT ACAGGAGCAG AAAATGATAA AATAGAAAAA 
TCTGTTCTTA ACTGCATTTA TAAGGTATTA CATGGGTGTG TTTATGGAGC TCAGTGTTTT 
AGCAATCCGA GGTATTTTCC AATAAGCCTT TCTGATTTGG CATCTGTAGA TTATGATCCT 
TTTATGCATT TGGAAAGCCT AAAAGAGCCT GAACCTTTGC ACTCTCCTGA TTCGGAACGA 
TCTTCTAAAC TCCAGCCAGT AACAGAAGTG AAAACTCAGA TGCAGCAAGG ATTAATCTCC 
ATAGCTGCCC GCACTGTTAT CACACATCTG GTGAATCACT TGGGCCATTA TCCAATGAGC 
GGTGGTCCTG CTATGTTAAC AAGTCAGGTG TGTGAGAATC ACGACAATCA TTACAGTGAA 
AGCACTGAAC TTTCCCCTGA ACTCTTCGAG AGTCCAAATA TTCAGTTTTT TGTGTTAAAC 
AATACAACCT TAGTGTCCTG TATCCAGATC AGAGCTGAAG AGAGTATACC TGGAGGAGGT 
TTATCTGCTG GTCTTGCATC AGCCAATTCA AATGTCAGAA TTATAGTACG TGATCTTTCT 
GGAAAATATT CATGGGATTC TGCCATCCTG TATGGACCAC CTCTTGTAAG TGGTCTGTCA 
GAACCTACAT CTCTCGTACT TTCATTGTCT CACCAAGAGA AGCCAGAAGA ACCTTCTGCA 
TCTAATGAAT GCTTAGAAGA TATAACAGTT AAAGATGGGA TTTCCCTCCA GTTTAAAAGA 
AGTTTTAGAG AAACTGTACC AACTTGGGAC ACAATCAGAG ATGAAGAAGA TGTTCTTGAT 
GAGCTTTTGC AGTATTTAGG TGTAACCAGT CCTGAATGCT TACAGAGAAC TGGAGTCTCA 
CTTAATATTC CTGCTCCACA GCCTGTGTGC ATTTCTGAAA AACAGGAGAA TGATGTTATT 
AATGCTATCC TTAAGCAGCA TACAGAGGAA AAAGAATTTG TTGAGAAACA CTTTAATGAC 
TTAAACATGA AAGCTGCAGA ACAAGATGAA CCAACACCTC AGAAACCTCA GTCAGCATTT 
TATTATTGCA GATTGCTTCT TAGTATATTG GGAATGAATT CCTGGGACAA AAGGAGGAGC 
TTTCATCTCC TGAAGAAAAA TGAAAAACTA CTTAGAGAAC TTAGGAACTT GGACTCAAGA 
CAGTGCCGAG AGACACACAA GATTGCAGTA TTTTATGTTG CTGAAGGACA AGAAGACAAA 
CATTCCATTC TCACCAATAC AGGAGGAAGT CAAGCATATG AAGATTTTGT AGCTGGTCTT 
GGTTGGGAGG TAAATCTTAC AAACCATTGT GGTTTCATGG GAGGACTACA AAAAAATAAA 
AGCACTGGAC TGACCACACC GTATTTTGCT ACCTCTACAG TTGAAGTAAT ATTTCATGTG 
TCAACAAGAA TGCCTTCAGA TTCTGATGAC TCTTTGACCA AAAAATTGAG ACATTTAGGA 
AATGATGAAG TGCACATTGT TTGGTCAGAG CATACTAGAG ACTACAGGAG AGGAATTATT 
CCTACAGAAT TTGGTGATGT CCTTATTGTT ATATACCCGA TGAAAAACCA CATGTTCAGT 
ATCCAGATAA TGAAAAAACC AGAGGTTCCC TTCTTTGGTC CACTCTTTGA TGGTGCTATT 
GTGAATGGAA AGGTCCTACC TATTATGGTT CGAGCAACAG CTATAAATGC AAGCCGTGCT 
CTGAAATCCC TGATTCCATT GTATCAAAAC TTCTATGAGG AGAGAGCACG ATATCTGCAA 
ACGATTGTCC AGCACCATTT AGAGCCAACG ACATTTGAAG ATTTTGCAGC ACAGGTTTTT 
TCTCCAGCTC CCTACCATCA TTTACCATCT GATGCCGTGG GCTCCTACCC AGAGATCCTA 
TCCAGTGAAA CTCCCACAGC GGCGCAGGTA GACGGGGCCG ACCTGGCCTC TCCAATGTCT 
CCTCGAACGA GCAAAAGCCG CATGTCCATG AAGCTGCGTC GCTCCTCTGG CTCCGCCAAT 
AAGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986190.1
CDS	172..6660
Translation	MRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQENKSENTTSFVGTPENIQFQKEPNSTVFTNISAPNQLDSFTKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDTTLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILSSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_012130800.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130800.1

ORF Insert Sequence:

ATGAGAAAAG TGGTAAAAGT ATATCAAGAA TGGATCCAGC AAGAGGAAAA ACCTTTGTTC 
ATGCAAGAGC CTGAAGAAAT TGTGATCTCT TCTTCAGACC TTCCGTGCAT TGACAATGTC 
ACAGACCATG ATATTTCAAT GGAAGAAGGA GAAAAAAGAG AAGAGGAAAA TGGGACCAAT 
GTTGCTGATC ATGTTCGAAA TTCCACTTGG CCTAAAAATG GAACCTACCA AGATATTCTT 
CATAATGCTT CTGAAGAAGC CATAGAACAA AACATACGAG CTGGTGCCCA GGCAGTTTTG 
CAGGTGTTTA TTATAAATTC TTCAAACATA TTTCTTCTTG AACCTGCAAA TGAGATAAAA 
AGTCTTCTGG ATGAGCACAC AGATATGTGT AAACGAATTC TTAACATTTA TCGGTTCATG 
GTTGTGCAAG TATCAATGGA CAAAAAGACT TGGGAACAGA TGCTGCTTGT GTTGCTCAGA 
GTCACGGAGT CTGTACTGAA GATGCCATCA CAAGCTTTTC TACAGTTCCA GGGGAAAAAA 
AACATGACTT TGGCAGGTCG ACTTGCAGGA CCACTTTTCC AGACTCTTAT AGTTGCCTGG 
ATTAAAGCAA ATCTAAATGT GTACATCTCT CGAGAACTTT GGGATGACTT ACTCTCAGTA 
TTGTCATCGT TGACCTATTG GGAAGAGTTA GCCACTGAAT GGTCGCTGAC TATGGAGACA 
CTAACTAAAG TTTTAGCTAG AAATTTGTAT AGTTTGGATC TCAGTGATTT ACCATTGGAT 
AAGCTGAGTG AACAGAAACA AAAAAAGCAC AAAGGGAAAG GAGTGGGACA TGAATTTCAG 
AAAGTTTCAG TTGACAAGTC ATTTTCTAGA GGATGGAGTC GCGATCAGCC TGGCCAAGCC 
CCAATGAGGC AGAGAAGTGC AACAACCACT GGTTCCCCAG GCACAGAAAA GGCAAGGAGT 
ATAGTACGGC AAAAAACTGT TGCCATGAGA AGCCGATCCA TTGGTGAATG TGCTCTGCCA 
TCGGCCTATA TACGCAGTGC TAAAAGTGCT CCTGTTCTGA TCCATACTTC CAAACCCTTC 
TTGCCTGATA TTGTTCTCAC TCCCCTTTCT GATGAGCTTT CAGATATTGA TGATGCTCAA 
ATACTTCCTC GTCCAACTAG AGTCAGACAT TTTTCGCAAA GTGAAGACAC TGGAAATGAA 
GTTTTTGGTG CTTTGAGTGA GGAGCAGCCA TTGCCTCGAA GTAGCAGCAC TTCTGACATC 
TTGGAACCAT TCACTGTTGA ACGAGCCAAA GGTGCAGTTC CTGTCATTGA CAGTTCATCC 
CGTCATGCAC CAAGCTTGCA GAGTTCCACA GAGGCTTCTT CAATAACTAG ATCCACTGAG 
AGCCACATCA CTGATACTCA TAGTAGAGAG TCTTCTCTGG AAGTTGGTGA TAGCATATAT 
GACCATCTCT GTCACTTAAT AGATCCAGTA GAACTTGCAG ATTCAGCTTT TGAACAAATC 
CAGTACATTG ACCTTGAAGG AGATGATGAT CTTCTTTCCT CCCTGAAAGA ATATTTTAAG 
GAAACCCAAG AAAGTCACAG TAAAAATGAG TTAGATAAAG ATGCAGCTTC GCAGGAAGTG 
ATCTCTGTTG CAGTAAATAA GGATGAAAGA TCATCCTCAG ATAAATTAGA GTACATAGAC 
CAGGAAAATA AATCAGAAAA TACCACCTCT TTTGTTGGAA CTCCTGAAAA TATACAATTT 
CAAAAAGAAC CAAACTCTAC TGTCTTCACA AATATTAGTG CACCCAACCA GTTAGACAGC 
TTTACGAAAA CAAAAACTTC TGAAAAATGT AAACAACTAA ACAATGATAA ACAGTCATCG 
GAACCTAGTT CAGGTAGCCC TTGTGATAAA GAAAAGAGAA AACATCTGTA TAGACAAACA 
GCCACAGAAT TAGATGCCTG TATAGATACC ACACTAGCTG AAAAGGTGAA GGGTGTGCAA 
ATTAATGAAA AAATCACTGT CCCACATGTT TTGCATGCCA AGAAAACTAC ACTAAAAGCA 
CCTGTTAACC GTAGAATGCC TCATGTTACA AGCACTAGCA AGCTTTCTCC AACTAAAAGG 
AGCTTGTCTG AAACAGTAAC TCACAGAGCC AAAATCATGA AAATTGCTAC TAAAAAGCGA 
AATAGTGTTC ATGTTACTTT TAGACCTTCT ACTGAGTCAG TTCAGTTTTA TAATCCTCTG 
GAAAACAAAG AGGCTCCATG GAAGATGAGA CTGCGGAAGC TCAGTGGCTT TAGTGGTAGT 
AATAGCAACA TCAGCAACCC CCACACCAGC TCCAACAGTG CTGCAGAGCT AGTAAAACCT 
GGTGTGTACA GGCCTCTAGA TACAATTAGT GCAGCATCAG CAAGCAGTGA AACAATTAAG 
GAAACGACAG AGATTCCCAC CGCCATATTA CAGAAAGAAG GAATTGCAAC TAATCAGTTA 
GGTAGTCGTA GTACTATTAA GTCATCAAGT CACGAGGCAG GACTACCGCA GGGCTCCCTG 
GGTGGCGTTT ATAAAACTGT TGTCCATGCT CTTTCGAAGC CGAAGGCAAA TGTTTCCCCA 
CAGAGGCAGA ACAGAATGCC ACCAGAGGCT CCACTGAGGG ATCTGTACAG TCATGTAATG 
GGCTATTTTG GAAGGAAAGC TGCAGTCAAT AAAGAGGACA TGAGCACAAA ACTGCCTCCT 
CTTAATAGTG ATATTGGCAG CAGCAATGCT AATGTTCCTG ATCTGATGGA TGAGTTTATA 
GCAGAACGAC TTCGAAGTGG TAATGCCTCA ACTATGACAC GAAGAGGAAG TAGTCCAGGT 
AGCCTCGAAA TTCCCAAAGA CCTCCCTGAT ATTCTAAATA AGCAAAACCA AATGCGTCCT 
ATTGATGACC CAGGTGTACC CTCAGAATGG ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT 
CTTATCAGCT CAGATAGTCA TTCGGATTCT TTCAGCGCTT TCCAGTATGA TGGCCGAAAA 
TTTGACAATT TTGGTTTTGG AGCTGATACT GGGAATGCAT CCTCTGCTGA CATGGATTCA 
GGTTCTGGCC ATTATCAGAG TGCTGAAGAG CAAGAGGTGG CTAGTCTAAC CACACTACAC 
ATTGACTCAG AAACAAGCAG TCTTAATCAA CAAGCTTTCT GTACTGAAGT TGCAACGGTT 
ACTGGTTCAG AAAGTGCCTC TCCAGTCCAT TCAGCTCTGG GATCCAGGTC ACAGACACCT 
TCTCCTTCCA CACTGAATAT AGATCACATG GAACAGAAGG ATCTACAGCT CGATGAGAAG 
CTCCACCACT CTGTTCTTCA GACACCAGAT GACCTAGAAA TCAGTGAATT TCCATCTGAA 
TGCTGTAGTG TGATGGCAGG GGGCACTCTT ACAGGATGGC ATGCTGATGT TGCTACTGTA 
ATGTGGCGAA GGATGCTGGG TATTTTGGGA GATGTCAACG CTATTATGGA TCCTGAAATA 
CATGCTCAAG TTTTTGATTA CCTCTGTGAA CTTTGGCAGA ATCTAGCTAA GATTAGAGAT 
AATCTTGGCA TTTCAACTGA TAACCTGACC TCCCCTTCTC CACCGGTTTT AATTCCTCCA 
CTGAGAATTC TTACACCTTG GCTTTTTAAG GCAACCATGT TAACTGATAA ATATAAACAA 
GGTAAATTAC ATGCCTATAA ACTTATTTGT AATACAATGA AAAGAAGACA AGATGTTTCT 
CCAAATAGAG ATTTTCTAAC ACATTTCTAC AACATAATGC ATTGTGGATT GCTTCACATT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCAC CTCAATTTTT CTCACTTGGT 
TTGCCTGGTG CAACAATGCT TATTATGGAT TTTATTGTAG CAGCTGGTAG AGTGGCTTCT 
TCAGCTTTTC TCAATGCACC AAGAGTGGAA GCACAAGTTC TTCTGGGATC TTTGGTTTGC 
TTTCCCAACT TGTATTGTGA ACTGCCTGCT CTCCATCCCA ACATCCCTGA TATTGCTGTG 
TCTCAATTTA CAGATGTTAA GGAACTTATA ATCAAAACTG TGTTAAGCTC TGCAAGAGAT 
GAGCCCTCTG GTCCTGCACG ATGTGTAGCA CTTTGCAGTT TAGGTATTTG GATTTGTGAA 
GAACTAGTCC ATGAGTCTCA TCATCCTCAA ATTAAGGAAG CTCTGAATGT AATTTGTGTT 
TCCTTAAAGT TTACTAATAA AACAGTAGCC CATGTAGCTT GTAACATGCT TCACATGCTG 
GTTCATTATG TACCTAGACT TCAGATTTAC CAGCCTGATT CTCCCTTGAA AATTATTCAA 
ATTCTAATAG CCACTATTAC CCATCTTTTG CCAAGTACGG AAGCGTCATC TTATGAAATG 
GACAAGAGGT TGGTGGTATC CTTACTTCTG TGTCTTCTGG ACTGGATCAT GGCCTTACCT 
CTTAAGACAC TGCTCCAACC AGTCCATGCT ACAGGAGCAG AAAATGATAA AATAGAAAAA 
TCTGTTCTTA ACTGCATTTA TAAGGTATTA CATGGGTGTG TTTATGGAGC TCAGTGTTTT 
AGCAATCCGA GGTATTTTCC AATAAGCCTT TCTGATTTGG CATCTGTAGA TTATGATCCT 
TTTATGCATT TGGAAAGCCT AAAAGAGCCT GAACCTTTGC ACTCTCCTGA TTCGGAACGA 
TCTTCTAAAC TCCAGCCAGT AACAGAAGTG AAAACTCAGA TGCAGCAAGG ATTAATCTCC 
ATAGCTGCCC GCACTGTTAT CACACATCTG GTGAATCACT TGGGCCATTA TCCAATGAGC 
GGTGGTCCTG CTATGTTAAC AAGTCAGGTG TGTGAGAATC ACGACAATCA TTACAGTGAA 
AGCACTGAAC TTTCCCCTGA ACTCTTCGAG AGTCCAAATA TTCAGTTTTT TGTGTTAAAC 
AATACAACCT TAGTGTCCTG TATCCAGATC AGAGCTGAAG AGAGTATACC TGGAGGAGGT 
TTATCTGCTG GTCTTGCATC AGCCAATTCA AATGTCAGAA TTATAGTACG TGATCTTTCT 
GGAAAATATT CATGGGATTC TGCCATCCTG TATGGACCAC CTCTTGTAAG TGGTCTGTCA 
GAACCTACAT CTCTCGTACT TTCATTGTCT CACCAAGAGA AGCCAGAAGA ACCTTCTGCA 
TCTAATGAAT GCTTAGAAGA TATAACAGTT AAAGATGGGA TTTCCCTCCA GTTTAAAAGA 
AGTTTTAGAG AAACTGTACC AACTTGGGAC ACAATCAGAG ATGAAGAAGA TGTTCTTGAT 
GAGCTTTTGC AGTATTTAGG TGTAACCAGT CCTGAATGCT TACAGAGAAC TGGAGTCTCA 
CTTAATATTC CTGCTCCACA GCCTGTGTGC ATTTCTGAAA AACAGGAGAA TGATGTTATT 
AATGCTATCC TTAAGCAGCA TACAGAGGAA AAAGAATTTG TTGAGAAACA CTTTAATGAC 
TTAAACATGA AAGCTGCAGA ACAAGATGAA CCAACACCTC AGAAACCTCA GTCAGCATTT 
TATTATTGCA GATTGCTTCT TAGTATATTG GGAATGAATT CCTGGGACAA AAGGAGGAGC 
TTTCATCTCC TGAAGAAAAA TGAAAAACTA CTTAGAGAAC TTAGGAACTT GGACTCAAGA 
CAGTGCCGAG AGACACACAA GATTGCAGTA TTTTATGTTG CTGAAGGACA AGAAGACAAA 
CATTCCATTC TCACCAATAC AGGAGGAAGT CAAGCATATG AAGATTTTGT AGCTGGTCTT 
GGTTGGGAGG TAAATCTTAC AAACCATTGT GGTTTCATGG GAGGACTACA AAAAAATAAA 
AGCACTGGAC TGACCACACC GTATTTTGCT ACCTCTACAG TTGAAGTAAT ATTTCATGTG 
TCAACAAGAA TGCCTTCAGA TTCTGATGAC TCTTTGACCA AAAAATTGAG ACATTTAGGA 
AATGATGAAG TGCACATTGT TTGGTCAGAG CATACTAGAG ACTACAGGAG AGGAATTATT 
CCTACAGAAT TTGGTGATGT CCTTATTGTT ATATACCCGA TGAAAAACCA CATGTTCAGT 
ATCCAGATAA TGAAAAAACC AGAGGTTCCC TTCTTTGGTC CACTCTTTGA TGGTGCTATT 
GTGAATGGAA AGGTCCTACC TATTATGGTT CGAGCAACAG CTATAAATGC AAGCCGTGCT 
CTGAAATCCC TGATTCCATT GTATCAAAAC TTCTATGAGG AGAGAGCACG ATATCTGCAA 
ACGATTGTCC AGCACCATTT AGAGCCAACG ACATTTGAAG ATTTTGCAGC ACAGGTTTTT 
TCTCCAGCTC CCTACCATCA TTTACCATCT GATGCCGTGG GCTCCTACCC AGAGATCCTA 
TCCAGTGAAA CTCCCACAGC GGCGCAGGTA GACGGGGCCG ACCTGGCCTC TCCAATGTCT 
CCTCGAACGA GCAAAAGCCG CATGTCCATG AAGCTGCGTC GCTCCTCTGG CTCCGCCAAT 
AAGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_012130801.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986191.1 Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X25, mRNA.

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CloneID	OOv14062
Clone ID Related Accession (Same CDS sequence)	XM_012130801.2
Accession Version	XM_012130801.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	ral GTPase-activating protein subunit alpha-1 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942367.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012130801.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCGCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAATGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTGACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GCCATGAGAA GCCGATCCAT TGGTGAATGT 
GCTCTGCCAT CGGCCTATAT ACGCAGTGCT AAAAGTGCTC CTGTTCTGAT CCATACTTCC 
AAACCCTTCT TGCCTGATAT TGTTCTCACT CCCCTTTCTG ATGAGCTTTC AGATATTGAT 
GATGCTCAAA TACTTCCTCG TCCAACTAGA GTCAGACATT TTTCGCAAAG TGAAGACACT 
GGAAATGAAG TTTTTGGTGC TTTGAGTGAG GAGCAGCCAT TGCCTCGAAG TAGCAGCACT 
TCTGACATCT TGGAACCATT CACTGTTGAA CGAGCCAAAG TCAATAAAGA GGACATGAGC 
ACAAAACTGC CTCCTCTTAA TAGTGATATT GGCAGCAGCA ATGCTAATGT TCCTGATCTG 
ATGGATGAGT TTATAGCAGA ACGACTTCGA AGTGGTAATG CCTCAACTAT GACACGAAGA 
GGAAGTAGTC CAGGTAGCCT CGAAATTCCC AAAGACCTCC CTGATATTCT AAATAAGCAA 
AACCAAATGC GTCCTATTGA TGACCCAGGT GTACCCTCAG AATGGACTTC TCCTGCCAGT 
GCAGGGAGCA GTGATCTTAT CAGCTCAGAT AGTCATTCGG ATTCTTTCAG CGCTTTCCAG 
TATGATGGCC GAAAATTTGA CAATTTTGGT TTTGGAGCTG ATACTGGGAA TGCATCCTCT 
GCTGACATGG ATTCAGGTTC TGGCCATTAT CAGAGTGCTG AAGAGCAAGA GGTGGCTAGT 
CTAACCACAC TACACATTGA CTCAGAAACA AGCAGTCTTA ATCAACAAGC TTTCTGTACT 
GAAGTTGCAA CGGTTACTGG TTCAGAAAGT GCCTCTCCAG TCCATTCAGC TCTGGGATCC 
AGGTCACAGA CACCTTCTCC TTCCACACTG AATATAGATC ACATGGAACA GAAGGATCTA 
CAGCTCGATG AGAAGCTCCA CCACTCTGTT CTTCAGACAC CAGATGACCT AGAAATCAGT 
GAATTTCCAT CTGAATGCTG TAGTGTGATG GCAGGGGGCA CTCTTACAGG ATGGCATGCT 
GATGTTGCTA CTGTAATGTG GCGAAGGATG CTGGGTATTT TGGGAGATGT CAACGCTATT 
ATGGATCCTG AAATACATGC TCAAGTTTTT GATTACCTCT GTGAACTTTG GCAGAATCTA 
GCTAAGATTA GAGATAATCT TGGCATTTCA ACTGATAACC TGACCTCCCC TTCTCCACCG 
GTTTTAATTC CTCCACTGAG AATTCTTACA CCTTGGCTTT TTAAGGCAAC CATGTTAACT 
GATAAATATA AACAAGGTAA ATTACATGCC TATAAACTTA TTTGTAATAC AATGAAAAGA 
AGACAAGATG TTTCTCCAAA TAGAGATTTT CTAACACATT TCTACAACAT AATGCATTGT 
GGATTGCTTC ACATTGATCA GGATATTGTC AATACAATCA TCAAGCACTG CTCACCTCAA 
TTTTTCTCAC TTGGTTTGCC TGGTGCAACA ATGCTTATTA TGGATTTTAT TGTAGCAGCT 
GGTAGAGTGG CTTCTTCAGC TTTTCTCAAT GCACCAAGAG TGGAAGCACA AGTTCTTCTG 
GGATCTTTGG TTTGCTTTCC CAACTTGTAT TGTGAACTGC CTGCTCTCCA TCCCAACATC 
CCTGATATTG CTGTGTCTCA ATTTACAGAT GTTAAGGAAC TTATAATCAA AACTGTGTTA 
AGCTCTGCAA GAGATGAGCC CTCTGGTCCT GCACGATGTG TAGCACTTTG CAGTTTAGGT 
ATTTGGATTT GTGAAGAACT AGTCCATGAG TCTCATCATC CTCAAATTAA GGAAGCTCTG 
AATGTAATTT GTGTTTCCTT AAAGTTTACT AATAAAACAG TAGCCCATGT AGCTTGTAAC 
ATGCTTCACA TGCTGGTTCA TTATGTACCT AGACTTCAGA TTTACCAGCC TGATTCTCCC 
TTGAAAATTA TTCAAATTCT AATAGCCACT ATTACCCATC TTTTGCCAAG TACGGAAGCG 
TCATCTTATG AAATGGACAA GAGGTTGGTG GTATCCTTAC TTCTGTGTCT TCTGGACTGG 
ATCATGGCCT TACCTCTTAA GACACTGCTC CAACCAGTCC ATGCTACAGG AGCAGAAAAT 
GATAAAATAG AAAAATCTGT TCTTAACTGC ATTTATAAGG TATTACATGG GTGTGTTTAT 
GGAGCTCAGT GTTTTAGCAA TCCGAGGTAT TTTCCAATAA GCCTTTCTGA TTTGGCATCT 
GTAGATTATG ATCCTTTTAT GCATTTGGAA AGCCTAAAAG AGCCTGAACC TTTGCACTCT 
CCTGATTCGG AACGATCTTC TAAACTCCAG CCAGTAACAG AAGTGAAAAC TCAGATGCAG 
CAAGGATTAA TCTCCATAGC TGCCCGCACT GTTATCACAC ATCTGGTGAA TCACTTGGGC 
CATTATCCAA TGAGCGGTGG TCCTGCTATG TTAACAAGTC AGGTGTGTGA GAATCACGAC 
AATCATTACA GTGAAAGCAC TGAACTTTCC CCTGAACTCT TCGAGAGTCC AAATATTCAG 
TTTTTTGTGT TAAACAATAC AACCTTAGTG TCCTGTATCC AGATCAGAGC TGAAGAGAGT 
ATACCTGGAG GAGGTTTATC TGCTGGTCTT GCATCAGCCA ATTCAAATGT CAGAATTATA 
GTACGTGATC TTTCTGGAAA ATATTCATGG GATTCTGCCA TCCTGTATGG ACCACCTCTT 
GTAAGTGGTC TGTCAGAACC TACATCTCTC GTACTTTCAT TGTCTCACCA AGAGAAGCCA 
GAAGAACCTT CTGCATCTAA TGAATGCTTA GAAGATATAA CAGTTAAAGA TGGGATTTCC 
CTCCAGTTTA AAAGAAGTTT TAGAGAAACT GTACCAACTT GGGACACAAT CAGAGATGAA 
GAAGATGTTC TTGATGAGCT TTTGCAGTAT TTAGGTGTAA CCAGTCCTGA ATGCTTACAG 
AGAACTGGAG TCTCACTTAA TATTCCTGCT CCACAGCCTG TGTGCATTTC TGAAAAACAG 
GAGAATGATG TTATTAATGC TATCCTTAAG CAGCATACAG AGGAAAAAGA ATTTGTTGAG 
AAACACTTTA ATGACTTAAA CATGAAAGCT GCAGAACAAG ATGAACCAAC ACCTCAGAAA 
CCTCAGTCAG CATTTTATTA TTGCAGATTG CTTCTTAGTA TATTGGGAAT GAATTCCTGG 
GACAAAAGGA GGAGCTTTCA TCTCCTGAAG AAAAATGAAA AACTACTTAG AGAACTTAGG 
AACTTGGACT CAAGACAGTG CCGAGAGACA CACAAGATTG CAGTATTTTA TGTTGCTGAA 
GGACAAGAAG ACAAACATTC CATTCTCACC AATACAGGAG GAAGTCAAGC ATATGAAGAT 
TTTGTAGCTG GTCTTGGTTG GGAGGTAAAT CTTACAAACC ATTGTGGTTT CATGGGAGGA 
CTACAAAAAA ATAAAAGCAC TGGACTGACC ACACCGTATT TTGCTACCTC TACAGTTGAA 
GTAATATTTC ATGTGTCAAC AAGAATGCCT TCAGATTCTG ATGACTCTTT GACCAAAAAA 
TTGAGACATT TAGGAAATGA TGAAGTGCAC ATTGTTTGGT CAGAGCATAC TAGAGACTAC 
AGGAGAGGAA TTATTCCTAC AGAATTTGGT GATGTCCTTA TTGTTATATA CCCGATGAAA 
AACCACATGT TCAGTATCCA GATAATGAAA AAACCAGAGG TTCCCTTCTT TGGTCCACTC 
TTTGATGGTG CTATTGTGAA TGGAAAGGTC CTACCTATTA TGGTTCGAGC AACAGCTATA 
AATGCAAGCC GTGCTCTGAA ATCCCTGATT CCATTGTATC AAAACTTCTA TGAGGAGAGA 
GCACGATATC TGCAAACGAT TGTCCAGCAC CATTTAGAGC CAACGACATT TGAAGATTTT 
GCAGCACAGG TTTTTTCTCC AGCTCCCTAC CATCATTTAC CATCTGATGC CGTGGGCTCC 
TACCCAGAGA TCCTATCCAG TGAAACTCCC ACAGCGGCGC AGGTAGACGG GGCCGACCTG 
GCCTCTCCAA TGTCTCCTCG AACGAGCAAA AGCCGCATGT CCATGAAGCT GCGTCGCTCC 
TCTGGCTCCG CCAATAAGTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986191.1
CDS	457..6900
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIGRIFTAEPPEKPREHENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILSSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_012130801.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130801.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCGCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAATGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTGACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GCCATGAGAA GCCGATCCAT TGGTGAATGT 
GCTCTGCCAT CGGCCTATAT ACGCAGTGCT AAAAGTGCTC CTGTTCTGAT CCATACTTCC 
AAACCCTTCT TGCCTGATAT TGTTCTCACT CCCCTTTCTG ATGAGCTTTC AGATATTGAT 
GATGCTCAAA TACTTCCTCG TCCAACTAGA GTCAGACATT TTTCGCAAAG TGAAGACACT 
GGAAATGAAG TTTTTGGTGC TTTGAGTGAG GAGCAGCCAT TGCCTCGAAG TAGCAGCACT 
TCTGACATCT TGGAACCATT CACTGTTGAA CGAGCCAAAG TCAATAAAGA GGACATGAGC 
ACAAAACTGC CTCCTCTTAA TAGTGATATT GGCAGCAGCA ATGCTAATGT TCCTGATCTG 
ATGGATGAGT TTATAGCAGA ACGACTTCGA AGTGGTAATG CCTCAACTAT GACACGAAGA 
GGAAGTAGTC CAGGTAGCCT CGAAATTCCC AAAGACCTCC CTGATATTCT AAATAAGCAA 
AACCAAATGC GTCCTATTGA TGACCCAGGT GTACCCTCAG AATGGACTTC TCCTGCCAGT 
GCAGGGAGCA GTGATCTTAT CAGCTCAGAT AGTCATTCGG ATTCTTTCAG CGCTTTCCAG 
TATGATGGCC GAAAATTTGA CAATTTTGGT TTTGGAGCTG ATACTGGGAA TGCATCCTCT 
GCTGACATGG ATTCAGGTTC TGGCCATTAT CAGAGTGCTG AAGAGCAAGA GGTGGCTAGT 
CTAACCACAC TACACATTGA CTCAGAAACA AGCAGTCTTA ATCAACAAGC TTTCTGTACT 
GAAGTTGCAA CGGTTACTGG TTCAGAAAGT GCCTCTCCAG TCCATTCAGC TCTGGGATCC 
AGGTCACAGA CACCTTCTCC TTCCACACTG AATATAGATC ACATGGAACA GAAGGATCTA 
CAGCTCGATG AGAAGCTCCA CCACTCTGTT CTTCAGACAC CAGATGACCT AGAAATCAGT 
GAATTTCCAT CTGAATGCTG TAGTGTGATG GCAGGGGGCA CTCTTACAGG ATGGCATGCT 
GATGTTGCTA CTGTAATGTG GCGAAGGATG CTGGGTATTT TGGGAGATGT CAACGCTATT 
ATGGATCCTG AAATACATGC TCAAGTTTTT GATTACCTCT GTGAACTTTG GCAGAATCTA 
GCTAAGATTA GAGATAATCT TGGCATTTCA ACTGATAACC TGACCTCCCC TTCTCCACCG 
GTTTTAATTC CTCCACTGAG AATTCTTACA CCTTGGCTTT TTAAGGCAAC CATGTTAACT 
GATAAATATA AACAAGGTAA ATTACATGCC TATAAACTTA TTTGTAATAC AATGAAAAGA 
AGACAAGATG TTTCTCCAAA TAGAGATTTT CTAACACATT TCTACAACAT AATGCATTGT 
GGATTGCTTC ACATTGATCA GGATATTGTC AATACAATCA TCAAGCACTG CTCACCTCAA 
TTTTTCTCAC TTGGTTTGCC TGGTGCAACA ATGCTTATTA TGGATTTTAT TGTAGCAGCT 
GGTAGAGTGG CTTCTTCAGC TTTTCTCAAT GCACCAAGAG TGGAAGCACA AGTTCTTCTG 
GGATCTTTGG TTTGCTTTCC CAACTTGTAT TGTGAACTGC CTGCTCTCCA TCCCAACATC 
CCTGATATTG CTGTGTCTCA ATTTACAGAT GTTAAGGAAC TTATAATCAA AACTGTGTTA 
AGCTCTGCAA GAGATGAGCC CTCTGGTCCT GCACGATGTG TAGCACTTTG CAGTTTAGGT 
ATTTGGATTT GTGAAGAACT AGTCCATGAG TCTCATCATC CTCAAATTAA GGAAGCTCTG 
AATGTAATTT GTGTTTCCTT AAAGTTTACT AATAAAACAG TAGCCCATGT AGCTTGTAAC 
ATGCTTCACA TGCTGGTTCA TTATGTACCT AGACTTCAGA TTTACCAGCC TGATTCTCCC 
TTGAAAATTA TTCAAATTCT AATAGCCACT ATTACCCATC TTTTGCCAAG TACGGAAGCG 
TCATCTTATG AAATGGACAA GAGGTTGGTG GTATCCTTAC TTCTGTGTCT TCTGGACTGG 
ATCATGGCCT TACCTCTTAA GACACTGCTC CAACCAGTCC ATGCTACAGG AGCAGAAAAT 
GATAAAATAG AAAAATCTGT TCTTAACTGC ATTTATAAGG TATTACATGG GTGTGTTTAT 
GGAGCTCAGT GTTTTAGCAA TCCGAGGTAT TTTCCAATAA GCCTTTCTGA TTTGGCATCT 
GTAGATTATG ATCCTTTTAT GCATTTGGAA AGCCTAAAAG AGCCTGAACC TTTGCACTCT 
CCTGATTCGG AACGATCTTC TAAACTCCAG CCAGTAACAG AAGTGAAAAC TCAGATGCAG 
CAAGGATTAA TCTCCATAGC TGCCCGCACT GTTATCACAC ATCTGGTGAA TCACTTGGGC 
CATTATCCAA TGAGCGGTGG TCCTGCTATG TTAACAAGTC AGGTGTGTGA GAATCACGAC 
AATCATTACA GTGAAAGCAC TGAACTTTCC CCTGAACTCT TCGAGAGTCC AAATATTCAG 
TTTTTTGTGT TAAACAATAC AACCTTAGTG TCCTGTATCC AGATCAGAGC TGAAGAGAGT 
ATACCTGGAG GAGGTTTATC TGCTGGTCTT GCATCAGCCA ATTCAAATGT CAGAATTATA 
GTACGTGATC TTTCTGGAAA ATATTCATGG GATTCTGCCA TCCTGTATGG ACCACCTCTT 
GTAAGTGGTC TGTCAGAACC TACATCTCTC GTACTTTCAT TGTCTCACCA AGAGAAGCCA 
GAAGAACCTT CTGCATCTAA TGAATGCTTA GAAGATATAA CAGTTAAAGA TGGGATTTCC 
CTCCAGTTTA AAAGAAGTTT TAGAGAAACT GTACCAACTT GGGACACAAT CAGAGATGAA 
GAAGATGTTC TTGATGAGCT TTTGCAGTAT TTAGGTGTAA CCAGTCCTGA ATGCTTACAG 
AGAACTGGAG TCTCACTTAA TATTCCTGCT CCACAGCCTG TGTGCATTTC TGAAAAACAG 
GAGAATGATG TTATTAATGC TATCCTTAAG CAGCATACAG AGGAAAAAGA ATTTGTTGAG 
AAACACTTTA ATGACTTAAA CATGAAAGCT GCAGAACAAG ATGAACCAAC ACCTCAGAAA 
CCTCAGTCAG CATTTTATTA TTGCAGATTG CTTCTTAGTA TATTGGGAAT GAATTCCTGG 
GACAAAAGGA GGAGCTTTCA TCTCCTGAAG AAAAATGAAA AACTACTTAG AGAACTTAGG 
AACTTGGACT CAAGACAGTG CCGAGAGACA CACAAGATTG CAGTATTTTA TGTTGCTGAA 
GGACAAGAAG ACAAACATTC CATTCTCACC AATACAGGAG GAAGTCAAGC ATATGAAGAT 
TTTGTAGCTG GTCTTGGTTG GGAGGTAAAT CTTACAAACC ATTGTGGTTT CATGGGAGGA 
CTACAAAAAA ATAAAAGCAC TGGACTGACC ACACCGTATT TTGCTACCTC TACAGTTGAA 
GTAATATTTC ATGTGTCAAC AAGAATGCCT TCAGATTCTG ATGACTCTTT GACCAAAAAA 
TTGAGACATT TAGGAAATGA TGAAGTGCAC ATTGTTTGGT CAGAGCATAC TAGAGACTAC 
AGGAGAGGAA TTATTCCTAC AGAATTTGGT GATGTCCTTA TTGTTATATA CCCGATGAAA 
AACCACATGT TCAGTATCCA GATAATGAAA AAACCAGAGG TTCCCTTCTT TGGTCCACTC 
TTTGATGGTG CTATTGTGAA TGGAAAGGTC CTACCTATTA TGGTTCGAGC AACAGCTATA 
AATGCAAGCC GTGCTCTGAA ATCCCTGATT CCATTGTATC AAAACTTCTA TGAGGAGAGA 
GCACGATATC TGCAAACGAT TGTCCAGCAC CATTTAGAGC CAACGACATT TGAAGATTTT 
GCAGCACAGG TTTTTTCTCC AGCTCCCTAC CATCATTTAC CATCTGATGC CGTGGGCTCC 
TACCCAGAGA TCCTATCCAG TGAAACTCCC ACAGCGGCGC AGGTAGACGG GGCCGACCTG 
GCCTCTCCAA TGTCTCCTCG AACGAGCAAA AGCCGCATGT CCATGAAGCT GCGTCGCTCC 
TCTGGCTCCG CCAATAAGTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_012130802.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986192.1 Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X26, mRNA.

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CloneID	OOv14063
Clone ID Related Accession (Same CDS sequence)	XM_012130802.2
Accession Version	XM_012130802.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	ral GTPase-activating protein subunit alpha-1 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942367.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012130802.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCGCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGTCAAT 
AAAGAGGACA TGAGCACAAA ACTGCCTCCT CTTAATAGTG ATATTGGCAG CAGCAATGCT 
AATGTTCCTG ATCTGATGGA TGAGTTTATA GCAGAACGAC TTCGAAGTGG TAATGCCTCA 
ACTATGACAC GAAGAGGAAG TAGTCCAGGT AGCCTCGAAA TTCCCAAAGA CCTCCCTGAT 
ATTCTAAATA AGCAAAACCA AATGCGTCCT ATTGATGACC CAGGTGTACC CTCAGAATGG 
ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT CTTATCAGCT CAGATAGTCA TTCGGATTCT 
TTCAGCGCTT TCCAGTATGA TGGCCGAAAA TTTGACAATT TTGGTTTTGG AGCTGATACT 
GGGAATGCAT CCTCTGCTGA CATGGATTCA GGTTCTGGCC ATTATCAGAG TGCTGAAGAG 
CAAGAGGTGG CTAGTCTAAC CACACTACAC ATTGACTCAG AAACAAGCAG TCTTAATCAA 
CAAGCTTTCT GTACTGAAGT TGCAACGGTT ACTGGTTCAG AAAGTGCCTC TCCAGTCCAT 
TCAGCTCTGG GATCCAGGTC ACAGACACCT TCTCCTTCCA CACTGAATAT AGATCACATG 
GAACAGAAGG ATCTACAGCT CGATGAGAAG CTCCACCACT CTGTTCTTCA GACACCAGAT 
GACCTAGAAA TCAGTGAATT TCCATCTGAA TGCTGTAGTG TGATGGCAGG GGGCACTCTT 
ACAGGATGGC ATGCTGATGT TGCTACTGTA ATGTGGCGAA GGATGCTGGG TATTTTGGGA 
GATGTCAACG CTATTATGGA TCCTGAAATA CATGCTCAAG TTTTTGATTA CCTCTGTGAA 
CTTTGGCAGA ATCTAGCTAA GATTAGAGAT AATCTTGGCA TTTCAACTGA TAACCTGACC 
TCCCCTTCTC CACCGGTTTT AATTCCTCCA CTGAGAATTC TTACACCTTG GCTTTTTAAG 
GCAACCATGT TAACTGATAA ATATAAACAA GGTAAATTAC ATGCCTATAA ACTTATTTGT 
AATACAATGA AAAGAAGACA AGATGTTTCT CCAAATAGAG ATTTTCTAAC ACATTTCTAC 
AACATAATGC ATTGTGGATT GCTTCACATT GATCAGGATA TTGTCAATAC AATCATCAAG 
CACTGCTCAC CTCAATTTTT CTCACTTGGT TTGCCTGGTG CAACAATGCT TATTATGGAT 
TTTATTGTAG CAGCTGGTAG AGTGGCTTCT TCAGCTTTTC TCAATGCACC AAGAGTGGAA 
GCACAAGTTC TTCTGGGATC TTTGGTTTGC TTTCCCAACT TGTATTGTGA ACTGCCTGCT 
CTCCATCCCA ACATCCCTGA TATTGCTGTG TCTCAATTTA CAGATGTTAA GGAACTTATA 
ATCAAAACTG TGTTAAGCTC TGCAAGAGAT GAGCCCTCTG GTCCTGCACG ATGTGTAGCA 
CTTTGCAGTT TAGGTATTTG GATTTGTGAA GAACTAGTCC ATGAGTCTCA TCATCCTCAA 
ATTAAGGAAG CTCTGAATGT AATTTGTGTT TCCTTAAAGT TTACTAATAA AACAGTAGCC 
CATGTAGCTT GTAACATGCT TCACATGCTG GTTCATTATG TACCTAGACT TCAGATTTAC 
CAGCCTGATT CTCCCTTGAA AATTATTCAA ATTCTAATAG CCACTATTAC CCATCTTTTG 
CCAAGTACGG AAGCGTCATC TTATGAAATG GACAAGAGGT TGGTGGTATC CTTACTTCTG 
TGTCTTCTGG ACTGGATCAT GGCCTTACCT CTTAAGACAC TGCTCCAACC AGTCCATGCT 
ACAGGAGCAG AAAATGATAA AATAGAAAAA TCTGTTCTTA ACTGCATTTA TAAGGTATTA 
CATGGGTGTG TTTATGGAGC TCAGTGTTTT AGCAATCCGA GGTATTTTCC AATAAGCCTT 
TCTGATTTGG CATCTGTAGA TTATGATCCT TTTATGCATT TGGAAAGCCT AAAAGAGCCT 
GAACCTTTGC ACTCTCCTGA TTCGGAACGA TCTTCTAAAC TCCAGCCAGT AACAGAAGTG 
AAAACTCAGA TGCAGCAAGG ATTAATCTCC ATAGCTGCCC GCACTGTTAT CACACATCTG 
GTGAATCACT TGGGCCATTA TCCAATGAGC GGTGGTCCTG CTATGTTAAC AAGTCAGGTG 
TGTGAGAATC ACGACAATCA TTACAGTGAA AGCACTGAAC TTTCCCCTGA ACTCTTCGAG 
AGTCCAAATA TTCAGTTTTT TGTGTTAAAC AATACAACCT TAGTGTCCTG TATCCAGATC 
AGAGCTGAAG AGAGTATACC TGGAGGAGGT TTATCTGCTG GTCTTGCATC AGCCAATTCA 
AATGTCAGAA TTATAGTACG TGATCTTTCT GGAAAATATT CATGGGATTC TGCCATCCTG 
TATGGACCAC CTCTTGTAAG TGGTCTGTCA GAACCTACAT CTCTCGTACT TTCATTGTCT 
CACCAAGAGA AGCCAGAAGA ACCTTCTGCA TCTAATGAAT GCTTAGAAGA TATAACAGTT 
AAAGATGGGA TTTCCCTCCA GTTTAAAAGA AGTTTTAGAG AAACTGTACC AACTTGGGAC 
ACAATCAGAG ATGAAGAAGA TGTTCTTGAT GAGCTTTTGC AGTATTTAGG TGTAACCAGT 
CCTGAATGCT TACAGAGAAC TGGAGTCTCA CTTAATATTC CTGCTCCACA GCCTGTGTGC 
ATTTCTGAAA AACAGGAGAA TGATGTTATT AATGCTATCC TTAAGCAGCA TACAGAGGAA 
AAAGAATTTG TTGAGAAACA CTTTAATGAC TTAAACATGA AAGCTGCAGA ACAAGATGAA 
CCAACACCTC AGAAACCTCA GTCAGCATTT TATTATTGCA GATTGCTTCT TAGTATATTG 
GGAATGAATT CCTGGGACAA AAGGAGGAGC TTTCATCTCC TGAAGAAAAA TGAAAAACTA 
CTTAGAGAAC TTAGGAACTT GGACTCAAGA CAGTGCCGAG AGACACACAA GATTGCAGTA 
TTTTATGTTG CTGAAGGACA AGAAGACAAA CATTCCATTC TCACCAATAC AGGAGGAAGT 
CAAGCATATG AAGATTTTGT AGCTGGTCTT GGTTGGGAGG TAAATCTTAC AAACCATTGT 
GGTTTCATGG GAGGACTACA AAAAAATAAA AGCACTGGAC TGACCACACC GTATTTTGCT 
ACCTCTACAG TTGAAGTAAT ATTTCATGTG TCAACAAGAA TGCCTTCAGA TTCTGATGAC 
TCTTTGACCA AAAAATTGAG ACATTTAGGA AATGATGAAG TGCACATTGT TTGGTCAGAG 
CATACTAGAG ACTACAGGAG AGGAATTATT CCTACAGAAT TTGGTGATGT CCTTATTGTT 
ATATACCCGA TGAAAAACCA CATGTTCAGT ATCCAGATAA TGAAAAAACC AGAGGTTCCC 
TTCTTTGGTC CACTCTTTGA TGGTGCTATT GTGAATGGAA AGGTCCTACC TATTATGGTT 
CGAGCAACAG CTATAAATGC AAGCCGTGCT CTGAAATCCC TGATTCCATT GTATCAAAAC 
TTCTATGAGG AGAGAGCACG ATATCTGCAA ACGATTGTCC AGCACCATTT AGAGCCAACG 
ACATTTGAAG ATTTTGCAGC ACAGGTTTTT TCTCCAGCTC CCTACCATCA TTTACCATCT 
GATGCCGTGG GCTCCTACCC AGAGATCCTA TCCAGTGAAA CTCCCACAGC GGCGCAGGTA 
GACGGGGCCG ACCTGGCCTC TCCAATGTCT CCTCGAACGA GCAAAAGCCG CATGTCCATG 
AAGCTGCGTC GCTCCTCTGG CTCCGCCAAT AAGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986192.1
CDS	457..6855
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILSSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_012130802.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130802.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCGCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGTCAAT 
AAAGAGGACA TGAGCACAAA ACTGCCTCCT CTTAATAGTG ATATTGGCAG CAGCAATGCT 
AATGTTCCTG ATCTGATGGA TGAGTTTATA GCAGAACGAC TTCGAAGTGG TAATGCCTCA 
ACTATGACAC GAAGAGGAAG TAGTCCAGGT AGCCTCGAAA TTCCCAAAGA CCTCCCTGAT 
ATTCTAAATA AGCAAAACCA AATGCGTCCT ATTGATGACC CAGGTGTACC CTCAGAATGG 
ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT CTTATCAGCT CAGATAGTCA TTCGGATTCT 
TTCAGCGCTT TCCAGTATGA TGGCCGAAAA TTTGACAATT TTGGTTTTGG AGCTGATACT 
GGGAATGCAT CCTCTGCTGA CATGGATTCA GGTTCTGGCC ATTATCAGAG TGCTGAAGAG 
CAAGAGGTGG CTAGTCTAAC CACACTACAC ATTGACTCAG AAACAAGCAG TCTTAATCAA 
CAAGCTTTCT GTACTGAAGT TGCAACGGTT ACTGGTTCAG AAAGTGCCTC TCCAGTCCAT 
TCAGCTCTGG GATCCAGGTC ACAGACACCT TCTCCTTCCA CACTGAATAT AGATCACATG 
GAACAGAAGG ATCTACAGCT CGATGAGAAG CTCCACCACT CTGTTCTTCA GACACCAGAT 
GACCTAGAAA TCAGTGAATT TCCATCTGAA TGCTGTAGTG TGATGGCAGG GGGCACTCTT 
ACAGGATGGC ATGCTGATGT TGCTACTGTA ATGTGGCGAA GGATGCTGGG TATTTTGGGA 
GATGTCAACG CTATTATGGA TCCTGAAATA CATGCTCAAG TTTTTGATTA CCTCTGTGAA 
CTTTGGCAGA ATCTAGCTAA GATTAGAGAT AATCTTGGCA TTTCAACTGA TAACCTGACC 
TCCCCTTCTC CACCGGTTTT AATTCCTCCA CTGAGAATTC TTACACCTTG GCTTTTTAAG 
GCAACCATGT TAACTGATAA ATATAAACAA GGTAAATTAC ATGCCTATAA ACTTATTTGT 
AATACAATGA AAAGAAGACA AGATGTTTCT CCAAATAGAG ATTTTCTAAC ACATTTCTAC 
AACATAATGC ATTGTGGATT GCTTCACATT GATCAGGATA TTGTCAATAC AATCATCAAG 
CACTGCTCAC CTCAATTTTT CTCACTTGGT TTGCCTGGTG CAACAATGCT TATTATGGAT 
TTTATTGTAG CAGCTGGTAG AGTGGCTTCT TCAGCTTTTC TCAATGCACC AAGAGTGGAA 
GCACAAGTTC TTCTGGGATC TTTGGTTTGC TTTCCCAACT TGTATTGTGA ACTGCCTGCT 
CTCCATCCCA ACATCCCTGA TATTGCTGTG TCTCAATTTA CAGATGTTAA GGAACTTATA 
ATCAAAACTG TGTTAAGCTC TGCAAGAGAT GAGCCCTCTG GTCCTGCACG ATGTGTAGCA 
CTTTGCAGTT TAGGTATTTG GATTTGTGAA GAACTAGTCC ATGAGTCTCA TCATCCTCAA 
ATTAAGGAAG CTCTGAATGT AATTTGTGTT TCCTTAAAGT TTACTAATAA AACAGTAGCC 
CATGTAGCTT GTAACATGCT TCACATGCTG GTTCATTATG TACCTAGACT TCAGATTTAC 
CAGCCTGATT CTCCCTTGAA AATTATTCAA ATTCTAATAG CCACTATTAC CCATCTTTTG 
CCAAGTACGG AAGCGTCATC TTATGAAATG GACAAGAGGT TGGTGGTATC CTTACTTCTG 
TGTCTTCTGG ACTGGATCAT GGCCTTACCT CTTAAGACAC TGCTCCAACC AGTCCATGCT 
ACAGGAGCAG AAAATGATAA AATAGAAAAA TCTGTTCTTA ACTGCATTTA TAAGGTATTA 
CATGGGTGTG TTTATGGAGC TCAGTGTTTT AGCAATCCGA GGTATTTTCC AATAAGCCTT 
TCTGATTTGG CATCTGTAGA TTATGATCCT TTTATGCATT TGGAAAGCCT AAAAGAGCCT 
GAACCTTTGC ACTCTCCTGA TTCGGAACGA TCTTCTAAAC TCCAGCCAGT AACAGAAGTG 
AAAACTCAGA TGCAGCAAGG ATTAATCTCC ATAGCTGCCC GCACTGTTAT CACACATCTG 
GTGAATCACT TGGGCCATTA TCCAATGAGC GGTGGTCCTG CTATGTTAAC AAGTCAGGTG 
TGTGAGAATC ACGACAATCA TTACAGTGAA AGCACTGAAC TTTCCCCTGA ACTCTTCGAG 
AGTCCAAATA TTCAGTTTTT TGTGTTAAAC AATACAACCT TAGTGTCCTG TATCCAGATC 
AGAGCTGAAG AGAGTATACC TGGAGGAGGT TTATCTGCTG GTCTTGCATC AGCCAATTCA 
AATGTCAGAA TTATAGTACG TGATCTTTCT GGAAAATATT CATGGGATTC TGCCATCCTG 
TATGGACCAC CTCTTGTAAG TGGTCTGTCA GAACCTACAT CTCTCGTACT TTCATTGTCT 
CACCAAGAGA AGCCAGAAGA ACCTTCTGCA TCTAATGAAT GCTTAGAAGA TATAACAGTT 
AAAGATGGGA TTTCCCTCCA GTTTAAAAGA AGTTTTAGAG AAACTGTACC AACTTGGGAC 
ACAATCAGAG ATGAAGAAGA TGTTCTTGAT GAGCTTTTGC AGTATTTAGG TGTAACCAGT 
CCTGAATGCT TACAGAGAAC TGGAGTCTCA CTTAATATTC CTGCTCCACA GCCTGTGTGC 
ATTTCTGAAA AACAGGAGAA TGATGTTATT AATGCTATCC TTAAGCAGCA TACAGAGGAA 
AAAGAATTTG TTGAGAAACA CTTTAATGAC TTAAACATGA AAGCTGCAGA ACAAGATGAA 
CCAACACCTC AGAAACCTCA GTCAGCATTT TATTATTGCA GATTGCTTCT TAGTATATTG 
GGAATGAATT CCTGGGACAA AAGGAGGAGC TTTCATCTCC TGAAGAAAAA TGAAAAACTA 
CTTAGAGAAC TTAGGAACTT GGACTCAAGA CAGTGCCGAG AGACACACAA GATTGCAGTA 
TTTTATGTTG CTGAAGGACA AGAAGACAAA CATTCCATTC TCACCAATAC AGGAGGAAGT 
CAAGCATATG AAGATTTTGT AGCTGGTCTT GGTTGGGAGG TAAATCTTAC AAACCATTGT 
GGTTTCATGG GAGGACTACA AAAAAATAAA AGCACTGGAC TGACCACACC GTATTTTGCT 
ACCTCTACAG TTGAAGTAAT ATTTCATGTG TCAACAAGAA TGCCTTCAGA TTCTGATGAC 
TCTTTGACCA AAAAATTGAG ACATTTAGGA AATGATGAAG TGCACATTGT TTGGTCAGAG 
CATACTAGAG ACTACAGGAG AGGAATTATT CCTACAGAAT TTGGTGATGT CCTTATTGTT 
ATATACCCGA TGAAAAACCA CATGTTCAGT ATCCAGATAA TGAAAAAACC AGAGGTTCCC 
TTCTTTGGTC CACTCTTTGA TGGTGCTATT GTGAATGGAA AGGTCCTACC TATTATGGTT 
CGAGCAACAG CTATAAATGC AAGCCGTGCT CTGAAATCCC TGATTCCATT GTATCAAAAC 
TTCTATGAGG AGAGAGCACG ATATCTGCAA ACGATTGTCC AGCACCATTT AGAGCCAACG 
ACATTTGAAG ATTTTGCAGC ACAGGTTTTT TCTCCAGCTC CCTACCATCA TTTACCATCT 
GATGCCGTGG GCTCCTACCC AGAGATCCTA TCCAGTGAAA CTCCCACAGC GGCGCAGGTA 
GACGGGGCCG ACCTGGCCTC TCCAATGTCT CCTCGAACGA GCAAAAGCCG CATGTCCATG 
AAGCTGCGTC GCTCCTCTGG CTCCGCCAAT AAGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015106613.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014962099.1 Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X27, mRNA.

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CloneID	OOv14064
Clone ID Related Accession (Same CDS sequence)	XM_015106613.1
Accession Version	XM_015106613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	ral GTPase-activating protein subunit alpha-1 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942367.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCGCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAATGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTGACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GATATTGATG ATGCTCAAAT ACTTCCTCGT 
CCAACTAGAG TCAGACATTT TTCGCAAAGT GAAGACACTG GAAATGAAGT TTTTGGTGCT 
TTGAGTGAGG AGCAGCCATT GCCTCGAAGT AGCAGCACTT CTGACATCTT GGAACCATTC 
ACTGTTGAAC GAGCCAAAGT CAATAAAGAG GACATGAGCA CAAAACTGCC TCCTCTTAAT 
AGTGATATTG GCAGCAGCAA TGCTAATGTT CCTGATCTGA TGGATGAGTT TATAGCAGAA 
CGACTTCGAA GTGGTAATGC CTCAACTATG ACACGAAGAG GAAGTAGTCC AGGTAGCCTC 
GAAATTCCCA AAGACCTCCC TGATATTCTA AATAAGCAAA ACCAAATGCG TCCTATTGAT 
GACCCAGGTG TACCCTCAGA ATGGACTTCT CCTGCCAGTG CAGGGAGCAG TGATCTTATC 
AGCTCAGATA GTCATTCGGA TTCTTTCAGC GCTTTCCAGT ATGATGGCCG AAAATTTGAC 
AATTTTGGTT TTGGAGCTGA TACTGGGAAT GCATCCTCTG CTGACATGGA TTCAGGTTCT 
GGCCATTATC AGAGTGCTGA AGAGCAAGAG GTGGCTAGTC TAACCACACT ACACATTGAC 
TCAGAAACAA GCAGTCTTAA TCAACAAGCT TTCTGTACTG AAGTTGCAAC GGTTACTGGT 
TCAGAAAGTG CCTCTCCAGT CCATTCAGCT CTGGGATCCA GGTCACAGAC ACCTTCTCCT 
TCCACACTGA ATATAGATCA CATGGAACAG AAGGATCTAC AGCTCGATGA GAAGCTCCAC 
CACTCTGTTC TTCAGACACC AGATGACCTA GAAATCAGTG AATTTCCATC TGAATGCTGT 
AGTGTGATGG CAGGGGGCAC TCTTACAGGA TGGCATGCTG ATGTTGCTAC TGTAATGTGG 
CGAAGGATGC TGGGTATTTT GGGAGATGTC AACGCTATTA TGGATCCTGA AATACATGCT 
CAAGTTTTTG ATTACCTCTG TGAACTTTGG CAGAATCTAG CTAAGATTAG AGATAATCTT 
GGCATTTCAA CTGATAACCT GACCTCCCCT TCTCCACCGG TTTTAATTCC TCCACTGAGA 
ATTCTTACAC CTTGGCTTTT TAAGGCAACC ATGTTAACTG ATAAATATAA ACAAGGTAAA 
TTACATGCCT ATAAACTTAT TTGTAATACA ATGAAAAGAA GACAAGATGT TTCTCCAAAT 
AGAGATTTTC TAACACATTT CTACAACATA ATGCATTGTG GATTGCTTCA CATTGATCAG 
GATATTGTCA ATACAATCAT CAAGCACTGC TCACCTCAAT TTTTCTCACT TGGTTTGCCT 
GGTGCAACAA TGCTTATTAT GGATTTTATT GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT 
TTTCTCAATG CACCAAGAGT GGAAGCACAA GTTCTTCTGG GATCTTTGGT TTGCTTTCCC 
AACTTGTATT GTGAACTGCC TGCTCTCCAT CCCAACATCC CTGATATTGC TGTGTCTCAA 
TTTACAGATG TTAAGGAACT TATAATCAAA ACTGTGTTAA GCTCTGCAAG AGATGAGCCC 
TCTGGTCCTG CACGATGTGT AGCACTTTGC AGTTTAGGTA TTTGGATTTG TGAAGAACTA 
GTCCATGAGT CTCATCATCC TCAAATTAAG GAAGCTCTGA ATGTAATTTG TGTTTCCTTA 
AAGTTTACTA ATAAAACAGT AGCCCATGTA GCTTGTAACA TGCTTCACAT GCTGGTTCAT 
TATGTACCTA GACTTCAGAT TTACCAGCCT GATTCTCCCT TGAAAATTAT TCAAATTCTA 
ATAGCCACTA TTACCCATCT TTTGCCAAGT ACGGAAGCGT CATCTTATGA AATGGACAAG 
AGGTTGGTGG TATCCTTACT TCTGTGTCTT CTGGACTGGA TCATGGCCTT ACCTCTTAAG 
ACACTGCTCC AACCAGTCCA TGCTACAGGA GCAGAAAATG ATAAAATAGA AAAATCTGTT 
CTTAACTGCA TTTATAAGGT ATTACATGGG TGTGTTTATG GAGCTCAGTG TTTTAGCAAT 
CCGAGGTATT TTCCAATAAG CCTTTCTGAT TTGGCATCTG TAGATTATGA TCCTTTTATG 
CATTTGGAAA GCCTAAAAGA GCCTGAACCT TTGCACTCTC CTGATTCGGA ACGATCTTCT 
AAACTCCAGC CAGTAACAGA AGTGAAAACT CAGATGCAGC AAGGATTAAT CTCCATAGCT 
GCCCGCACTG TTATCACACA TCTGGTGAAT CACTTGGGCC ATTATCCAAT GAGCGGTGGT 
CCTGCTATGT TAACAAGTCA GGTGTGTGAG AATCACGACA ATCATTACAG TGAAAGCACT 
GAACTTTCCC CTGAACTCTT CGAGAGTCCA AATATTCAGT TTTTTGTGTT AAACAATACA 
ACCTTAGTGT CCTGTATCCA GATCAGAGCT GAAGAGAGTA TACCTGGAGG AGGTTTATCT 
GCTGGTCTTG CATCAGCCAA TTCAAATGTC AGAATTATAG TACGTGATCT TTCTGGAAAA 
TATTCATGGG ATTCTGCCAT CCTGTATGGA CCACCTCTTG TAAGTGGTCT GTCAGAACCT 
ACATCTCTCG TACTTTCATT GTCTCACCAA GAGAAGCCAG AAGAACCTTC TGCATCTAAT 
GAATGCTTAG AAGATATAAC AGTTAAAGAT GGGATTTCCC TCCAGTTTAA AAGAAGTTTT 
AGAGAAACTG TACCAACTTG GGACACAATC AGAGATGAAG AAGATGTTCT TGATGAGCTT 
TTGCAGTATT TAGGTGTAAC CAGTCCTGAA TGCTTACAGA GAACTGGAGT CTCACTTAAT 
ATTCCTGCTC CACAGCCTGT GTGCATTTCT GAAAAACAGG AGAATGATGT TATTAATGCT 
ATCCTTAAGC AGCATACAGA GGAAAAAGAA TTTGTTGAGA AACACTTTAA TGACTTAAAC 
ATGAAAGCTG CAGAACAAGA TGAACCAACA CCTCAGAAAC CTCAGTCAGC ATTTTATTAT 
TGCAGATTGC TTCTTAGTAT ATTGGGAATG AATTCCTGGG ACAAAAGGAG GAGCTTTCAT 
CTCCTGAAGA AAAATGAAAA ACTACTTAGA GAACTTAGGA ACTTGGACTC AAGACAGTGC 
CGAGAGACAC ACAAGATTGC AGTATTTTAT GTTGCTGAAG GACAAGAAGA CAAACATTCC 
ATTCTCACCA ATACAGGAGG AAGTCAAGCA TATGAAGATT TTGTAGCTGG TCTTGGTTGG 
GAGGTAAATC TTACAAACCA TTGTGGTTTC ATGGGAGGAC TACAAAAAAA TAAAAGCACT 
GGACTGACCA CACCGTATTT TGCTACCTCT ACAGTTGAAG TAATATTTCA TGTGTCAACA 
AGAATGCCTT CAGATTCTGA TGACTCTTTG ACCAAAAAAT TGAGACATTT AGGAAATGAT 
GAAGTGCACA TTGTTTGGTC AGAGCATACT AGAGACTACA GGAGAGGAAT TATTCCTACA 
GAATTTGGTG ATGTCCTTAT TGTTATATAC CCGATGAAAA ACCACATGTT CAGTATCCAG 
ATAATGAAAA AACCAGAGGT TCCCTTCTTT GGTCCACTCT TTGATGGTGC TATTGTGAAT 
GGAAAGGTCC TACCTATTAT GGTTCGAGCA ACAGCTATAA ATGCAAGCCG TGCTCTGAAA 
TCCCTGATTC CATTGTATCA AAACTTCTAT GAGGAGAGAG CACGATATCT GCAAACGATT 
GTCCAGCACC ATTTAGAGCC AACGACATTT GAAGATTTTG CAGCACAGGT TTTTTCTCCA 
GCTCCCTACC ATCATTTACC ATCTGATGCC GTGGGCTCCT ACCCAGAGAT CCTATCCAGT 
GAAACTCCCA CAGCGGCGCA GGTAGACGGG GCCGACCTGG CCTCTCCAAT GTCTCCTCGA 
ACGAGCAAAA GCCGCATGTC CATGAAGCTG CGTCGCTCCT CTGGCTCCGC CAATAAGTCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014962099.1
CDS	457..6759
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIGRIFTAEPPEKPREHENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILSSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_015106613.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015106613.1

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCGCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAATGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTGACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GATATTGATG ATGCTCAAAT ACTTCCTCGT 
CCAACTAGAG TCAGACATTT TTCGCAAAGT GAAGACACTG GAAATGAAGT TTTTGGTGCT 
TTGAGTGAGG AGCAGCCATT GCCTCGAAGT AGCAGCACTT CTGACATCTT GGAACCATTC 
ACTGTTGAAC GAGCCAAAGT CAATAAAGAG GACATGAGCA CAAAACTGCC TCCTCTTAAT 
AGTGATATTG GCAGCAGCAA TGCTAATGTT CCTGATCTGA TGGATGAGTT TATAGCAGAA 
CGACTTCGAA GTGGTAATGC CTCAACTATG ACACGAAGAG GAAGTAGTCC AGGTAGCCTC 
GAAATTCCCA AAGACCTCCC TGATATTCTA AATAAGCAAA ACCAAATGCG TCCTATTGAT 
GACCCAGGTG TACCCTCAGA ATGGACTTCT CCTGCCAGTG CAGGGAGCAG TGATCTTATC 
AGCTCAGATA GTCATTCGGA TTCTTTCAGC GCTTTCCAGT ATGATGGCCG AAAATTTGAC 
AATTTTGGTT TTGGAGCTGA TACTGGGAAT GCATCCTCTG CTGACATGGA TTCAGGTTCT 
GGCCATTATC AGAGTGCTGA AGAGCAAGAG GTGGCTAGTC TAACCACACT ACACATTGAC 
TCAGAAACAA GCAGTCTTAA TCAACAAGCT TTCTGTACTG AAGTTGCAAC GGTTACTGGT 
TCAGAAAGTG CCTCTCCAGT CCATTCAGCT CTGGGATCCA GGTCACAGAC ACCTTCTCCT 
TCCACACTGA ATATAGATCA CATGGAACAG AAGGATCTAC AGCTCGATGA GAAGCTCCAC 
CACTCTGTTC TTCAGACACC AGATGACCTA GAAATCAGTG AATTTCCATC TGAATGCTGT 
AGTGTGATGG CAGGGGGCAC TCTTACAGGA TGGCATGCTG ATGTTGCTAC TGTAATGTGG 
CGAAGGATGC TGGGTATTTT GGGAGATGTC AACGCTATTA TGGATCCTGA AATACATGCT 
CAAGTTTTTG ATTACCTCTG TGAACTTTGG CAGAATCTAG CTAAGATTAG AGATAATCTT 
GGCATTTCAA CTGATAACCT GACCTCCCCT TCTCCACCGG TTTTAATTCC TCCACTGAGA 
ATTCTTACAC CTTGGCTTTT TAAGGCAACC ATGTTAACTG ATAAATATAA ACAAGGTAAA 
TTACATGCCT ATAAACTTAT TTGTAATACA ATGAAAAGAA GACAAGATGT TTCTCCAAAT 
AGAGATTTTC TAACACATTT CTACAACATA ATGCATTGTG GATTGCTTCA CATTGATCAG 
GATATTGTCA ATACAATCAT CAAGCACTGC TCACCTCAAT TTTTCTCACT TGGTTTGCCT 
GGTGCAACAA TGCTTATTAT GGATTTTATT GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT 
TTTCTCAATG CACCAAGAGT GGAAGCACAA GTTCTTCTGG GATCTTTGGT TTGCTTTCCC 
AACTTGTATT GTGAACTGCC TGCTCTCCAT CCCAACATCC CTGATATTGC TGTGTCTCAA 
TTTACAGATG TTAAGGAACT TATAATCAAA ACTGTGTTAA GCTCTGCAAG AGATGAGCCC 
TCTGGTCCTG CACGATGTGT AGCACTTTGC AGTTTAGGTA TTTGGATTTG TGAAGAACTA 
GTCCATGAGT CTCATCATCC TCAAATTAAG GAAGCTCTGA ATGTAATTTG TGTTTCCTTA 
AAGTTTACTA ATAAAACAGT AGCCCATGTA GCTTGTAACA TGCTTCACAT GCTGGTTCAT 
TATGTACCTA GACTTCAGAT TTACCAGCCT GATTCTCCCT TGAAAATTAT TCAAATTCTA 
ATAGCCACTA TTACCCATCT TTTGCCAAGT ACGGAAGCGT CATCTTATGA AATGGACAAG 
AGGTTGGTGG TATCCTTACT TCTGTGTCTT CTGGACTGGA TCATGGCCTT ACCTCTTAAG 
ACACTGCTCC AACCAGTCCA TGCTACAGGA GCAGAAAATG ATAAAATAGA AAAATCTGTT 
CTTAACTGCA TTTATAAGGT ATTACATGGG TGTGTTTATG GAGCTCAGTG TTTTAGCAAT 
CCGAGGTATT TTCCAATAAG CCTTTCTGAT TTGGCATCTG TAGATTATGA TCCTTTTATG 
CATTTGGAAA GCCTAAAAGA GCCTGAACCT TTGCACTCTC CTGATTCGGA ACGATCTTCT 
AAACTCCAGC CAGTAACAGA AGTGAAAACT CAGATGCAGC AAGGATTAAT CTCCATAGCT 
GCCCGCACTG TTATCACACA TCTGGTGAAT CACTTGGGCC ATTATCCAAT GAGCGGTGGT 
CCTGCTATGT TAACAAGTCA GGTGTGTGAG AATCACGACA ATCATTACAG TGAAAGCACT 
GAACTTTCCC CTGAACTCTT CGAGAGTCCA AATATTCAGT TTTTTGTGTT AAACAATACA 
ACCTTAGTGT CCTGTATCCA GATCAGAGCT GAAGAGAGTA TACCTGGAGG AGGTTTATCT 
GCTGGTCTTG CATCAGCCAA TTCAAATGTC AGAATTATAG TACGTGATCT TTCTGGAAAA 
TATTCATGGG ATTCTGCCAT CCTGTATGGA CCACCTCTTG TAAGTGGTCT GTCAGAACCT 
ACATCTCTCG TACTTTCATT GTCTCACCAA GAGAAGCCAG AAGAACCTTC TGCATCTAAT 
GAATGCTTAG AAGATATAAC AGTTAAAGAT GGGATTTCCC TCCAGTTTAA AAGAAGTTTT 
AGAGAAACTG TACCAACTTG GGACACAATC AGAGATGAAG AAGATGTTCT TGATGAGCTT 
TTGCAGTATT TAGGTGTAAC CAGTCCTGAA TGCTTACAGA GAACTGGAGT CTCACTTAAT 
ATTCCTGCTC CACAGCCTGT GTGCATTTCT GAAAAACAGG AGAATGATGT TATTAATGCT 
ATCCTTAAGC AGCATACAGA GGAAAAAGAA TTTGTTGAGA AACACTTTAA TGACTTAAAC 
ATGAAAGCTG CAGAACAAGA TGAACCAACA CCTCAGAAAC CTCAGTCAGC ATTTTATTAT 
TGCAGATTGC TTCTTAGTAT ATTGGGAATG AATTCCTGGG ACAAAAGGAG GAGCTTTCAT 
CTCCTGAAGA AAAATGAAAA ACTACTTAGA GAACTTAGGA ACTTGGACTC AAGACAGTGC 
CGAGAGACAC ACAAGATTGC AGTATTTTAT GTTGCTGAAG GACAAGAAGA CAAACATTCC 
ATTCTCACCA ATACAGGAGG AAGTCAAGCA TATGAAGATT TTGTAGCTGG TCTTGGTTGG 
GAGGTAAATC TTACAAACCA TTGTGGTTTC ATGGGAGGAC TACAAAAAAA TAAAAGCACT 
GGACTGACCA CACCGTATTT TGCTACCTCT ACAGTTGAAG TAATATTTCA TGTGTCAACA 
AGAATGCCTT CAGATTCTGA TGACTCTTTG ACCAAAAAAT TGAGACATTT AGGAAATGAT 
GAAGTGCACA TTGTTTGGTC AGAGCATACT AGAGACTACA GGAGAGGAAT TATTCCTACA 
GAATTTGGTG ATGTCCTTAT TGTTATATAC CCGATGAAAA ACCACATGTT CAGTATCCAG 
ATAATGAAAA AACCAGAGGT TCCCTTCTTT GGTCCACTCT TTGATGGTGC TATTGTGAAT 
GGAAAGGTCC TACCTATTAT GGTTCGAGCA ACAGCTATAA ATGCAAGCCG TGCTCTGAAA 
TCCCTGATTC CATTGTATCA AAACTTCTAT GAGGAGAGAG CACGATATCT GCAAACGATT 
GTCCAGCACC ATTTAGAGCC AACGACATTT GAAGATTTTG CAGCACAGGT TTTTTCTCCA 
GCTCCCTACC ATCATTTACC ATCTGATGCC GTGGGCTCCT ACCCAGAGAT CCTATCCAGT 
GAAACTCCCA CAGCGGCGCA GGTAGACGGG GCCGACCTGG CCTCTCCAAT GTCTCCTCGA 
ACGAGCAAAA GCCGCATGTC CATGAAGCTG CGTCGCTCCT CTGGCTCCGC CAATAAGTCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_012130803.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986193.1 Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X28, mRNA.

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CloneID	OOv14065
Clone ID Related Accession (Same CDS sequence)	XM_012130803.2
Accession Version	XM_012130803.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	ral GTPase-activating protein subunit alpha-1 isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942367.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012130803.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCGCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAGCCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGTGGG CTCCTACCCA 
GAGATCCTAT CCAGTGAAAC TCCCACAGCG GCGCAGGTAG ACGGGGCCGA CCTGGCCTCT 
CCAATGTCTC CTCGAACGAG CAAAAGCCGC ATGTCCATGA AGCTGCGTCG CTCCTCTGGC 
TCCGCCAATA AGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986193.1
CDS	456..6713
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILSSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_012130803.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130803.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCGCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAGCCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGTGGG CTCCTACCCA 
GAGATCCTAT CCAGTGAAAC TCCCACAGCG GCGCAGGTAG ACGGGGCCGA CCTGGCCTCT 
CCAATGTCTC CTCGAACGAG CAAAAGCCGC ATGTCCATGA AGCTGCGTCG CTCCTCTGGC 
TCCGCCAATA AGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_012130804.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986194.1 Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X29, mRNA.

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CloneID	OOv14066
Clone ID Related Accession (Same CDS sequence)	XM_012130804.2
Accession Version	XM_012130804.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	ral GTPase-activating protein subunit alpha-1 isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942367.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012130804.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCGCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAGCCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGGCTC CTACCCAGAG 
ATCCTATCCA GTGAAACTCC CACAGCGGCG CAGGTAGACG GGGCCGACCT GGCCTCTCCA 
ATGTCTCCTC GAACGAGCAA AAGCCGCATG TCCATGAAGC TGCGTCGCTC CTCTGGCTCC 
GCCAATAAGT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986194.1
CDS	456..6710
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAGSYPEILSSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_012130804.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130804.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCGCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAGCCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGGCTC CTACCCAGAG 
ATCCTATCCA GTGAAACTCC CACAGCGGCG CAGGTAGACG GGGCCGACCT GGCCTCTCCA 
ATGTCTCCTC GAACGAGCAA AAGCCGCATG TCCATGAAGC TGCGTCGCTC CTCTGGCTCC 
GCCAATAAGT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015102009.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014957495.1 Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X13, mRNA.

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CloneID	OSm53950
Clone ID Related Accession (Same CDS sequence)	XM_015102009.1
Accession Version	XM_015102009.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	ral GTPase-activating protein subunit alpha-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCC GGGGACAGGG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCCCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAACGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTAACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GATATTGATG ATGCTCAAAT ACTTCCTCGT 
CCAACTAGAG TCAGACATTT TTCGCAAAGT GAAGACACTG GAAATGAAGT TTTTGGTGCT 
TTGAGTGAGG AGCAGCCATT GCCTCGAAGT AGCAGCACTT CTGACATCTT GGAACCATTC 
ACTGTTGAAC GAGCCAAAGT CAATAAAGAG GACATGAGCA CAAAACTGCC TCCTCTTAAT 
AGTGATATTG GCAGCAGCAA TGCTAATGTT CCTGATCTGA TGGATGAGTT TATAGCAGAA 
CGACTTCGAA GTGGTAATGC CTCAACTATG ACACGAAGAG GAAGTAGTCC AGGTAGCCTC 
GAAATTCCCA AAGACCTCCC TGATATTCTA AATAAGCAAA ACCAAATGCG TCCTATTGAT 
GACCCAGGTG TACCCTCAGA ATGGACTTCT CCTGCCAGTG CAGGGAGCAG TGATCTTATC 
AGCTCAGATA GTCATTCGGA TTCTTTCAGC GCTTTCCAGT ATGATGGCCG AAAATTTGAC 
AATTTTGGTT TTGGAGCTGA TACTGGGAAT GCATCCTCTG CTGACATGGA TTCAGGTTCT 
GGCCATTATC AGAGTGCTGA AGAGCAAGAG GTGGCTAGTC TAACCACACT ACACATTGAC 
TCAGAAACAA GCAGTCTTAA TCAACAAGCT TTCTGTACTG AAGTTGCAAC GGTTACTGGT 
TCAGAAAGTG CCTCTCCAGT CCATTCAGCT CTGGGATCCA GGTCACAGAC ACCTTCTCCT 
TCCACACTGA ATATAGATCA CATGGAACAG AAGGATCTAC AGCTCGATGA GAAGCTCCAC 
CACTCTGTTC TTCAGACACC AGATGACCTA GAAATCAGTG AATTTCCATC TGAATGCTGT 
AGTGTGATGG CAGGGGGCAC TCTTACAGGA TGGCATGCTG ATGTTGCTAC TGTAATGTGG 
CGAAGGATGC TGGGTATTTT GGGAGATGTC AACGCTATTA TGGATCCTGA AATACATGCT 
CAAGTTTTTG ATTACCTCTG TGAACTTTGG CAGAATCTAG CTAAGATTAG AGATAATCTT 
GGCATTTCAA CTGATAACCT GACCTCCCCT TCTCCACCGG TTTTAATTCC TCCACTGAGA 
ATTCTTACAC CTTGGCTTTT TAAGGCAACC ATGTTAACTG ATAAATATAA ACAAGGTAAA 
TTACATGCCT ATAAACTTAT TTGTAATACA ATGAAAAGAA GACAAGATGT TTCTCCAAAT 
AGAGATTTTC TAACACATTT CTACAACATA ATGCATTGTG GATTGCTTCA CATTGATCAG 
GATATTGTCA ATACAATCAT CAAGCACTGC TCACCTCAAT TTTTCTCACT TGGTTTGCCT 
GGTGCAACAA TGCTTATTAT GGATTTTATT GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT 
TTTCTCAATG CACCAAGAGT GGAAGCACAA GTTCTTCTGG GATCTTTGGT TTGCTTTCCC 
AACTTGTATT GTGAACTGCC TGCTCTCCAT CCCAACATCC CTGATATTGC TGTGTCTCAA 
TTTACAGATG TTAAGGAACT TATAATCAAA ACTGTGTTAA GCTCTGCAAG AGATGAGCCC 
TCTGGTCCTG CACGATGTGT AGCACTTTGC AGTTTAGGTA TTTGGATTTG TGAAGAACTA 
GTCCATGAGT CTCATCATCC TCAAATTAAG GAAGCTCTGA ATGTAATTTG TGTTTCCTTA 
AAGTTTACTA ATAAAACAGT AGCCCATGTA GCTTGTAACA TGCTTCACAT GCTGGTTCAT 
TATGTACCTA GACTTCAGAT TTACCAGCCT GATTCTCCCT TGAAAATTAT TCAAATTCTA 
ATAGCCACTA TTACCCATCT TTTGCCAAGT ACGGAAGCGT CATCTTATGA AATGGACAAG 
AGGTTGGTGG TATCCTTACT TCTGTGTCTT CTGGACTGGA TCATGGCCTT ACCTCTTAAG 
ACACTGCTCC AACCAGTCCA TGCTACAGGA GCAGAAAATG ATAAAATAGA AAAATCTGTT 
CTTAACTGCA TTTATAAGGT ATTACATGGG TGTGTTTATG GAGCTCAGTG TTTTAGCAAT 
CCGAGGTATT TTCCAATAAG CCTTTCTGAT TTGGCATCTG TAGATTATGA TCCTTTTATG 
CATTTGGAAA GCCTAAAAGA GCCTGAACCT TTGCACTCTC CTGATTCGGA ACGATCTTCT 
AAACTCCAGC CAGTAACAGA AGTGAAAACT CAGATGCAGC AAGGATTAAT CTCCATAGCT 
GCCCGCACTG TTATCACACA TCTGGTGAAT CACTTGGGCC ATTATCCAAT GAGCGGTGGT 
CCTGCTATGT TAACAAGTCA GGTGTGTGAG AATCACGACA ATCATTACAG TGAAAGCACT 
GAACTTTCCC CTGAACTCTT CGAGAGTCCA AATATTCAGT TTTTTGTGTT AAACAATACA 
ACCTTAGTGT CCTGTATCCA GATCAGAGCT GAAGAGAGTA TACCTGGAGG AGGTTTATCT 
GCTGGTCTTG CATCAGCCAA TTCAAATGTC AGAATTATAG TACGTGATCT TTCTGGAAAA 
TATTCATGGG ATTCTGCCAT CCTGTATGGA CCACCTCTTG TAAGTGGTCT GTCAGAACCT 
ACATCTCTCG TACTTTCATT GTCTCACCAA GAGAAGCCAG AAGAACCTTC TGCATCTAAT 
GAATGCTTAG AAGATATAAC AGTTAAAGAT GGGATTTCCC TCCAGTTTAA AAGAAGTTTT 
AGAGAAACTG TACCAACTTG GGACACAATC AGAGATGAAG AAGATGTTCT TGATGAGCTT 
TTGCAGTATT TAGGTGTAAC CAGTCCTGAA TGCTTACAGA GAACTGGAGT CTCACTTAAT 
ATTCCTGCTC CACAGCCTGT GTGCATTTCT GAAAAACAGG AGAATGATGT TATTAATGCT 
ATCCTTAAGC AGCATACAGA GGAAAAAGAA TTTGTTGAGA AACACTTTAA TGACTTAAAC 
ATGAAAGCTG CAGAACAAGA TGAACCAACA CCTCAGAAAC CTCAGTCAGC ATTTTATTAT 
TGCAGATTGC TTCTTAGTAT ATTGGGAATG AATTCCTGGG ACAAAAGGAG GAGCTTTCAT 
CTCCTGAAGA AAAATGAAAA ACTACTTAGA GAACTTAGGA ACTTGGACTC AAGACAGTGC 
CGAGAGACAC ACAAGATTGC AGTATTTTAT GTTGCTGAAG GACAAGAAGA CAAACATTCC 
ATTCTCACCA ATACAGGAGG AAGTCAAGCA TATGAAGATT TTGTAGCTGG TCTTGGTTGG 
GAGGTAAATC TTACAAACCA TTGTGGTTTC ATGGGAGGAC TACAAAAAAA TAAAAGCACT 
GGACTGACCA CACCGTATTT TGCTACCTCT ACAGTTGAAG TAATATTTCA TGTGTCAACA 
AGAATGCCTT CAGATTCTGA TGACTCTTTG ACCAAAAAAT TGAGACATTT AGGAAATGAT 
GAAGTGCACA TTGTTTGGTC AGAGCATACT AGAGACTACA GGAGAGGAAT TATTCCTACA 
GAATTTGGTG ATGTCCTTAT TGTTATATAC CCGATGAAAA ACCACATGTT CAGTATCCAG 
ATAATGAAAA AACCAGAGGT TCCCTTCTTT GGTCCACTCT TTGATGGTGC TATTGTGAAT 
GGAAAGGTCC TACCTATTAT GGTTCGAGCA ACAGCTATAA ATGCAAGCCG TGCTCTGAAA 
TCCCTGATTC CATTGTATCA AAACTTCTAT GAGGAGAGAG CACGATATCT GCAAACGATT 
GTCCAGCACC ATTTAGAGCC AACGACATTT GAAGATTTTG CAGCACAGGT TTTTTCTCCA 
GCTCCCTACC ATCATTTACC ATCTGATGCC GTGGGCTCCT ACCCAGAGAT CCTATCCAGT 
GAAACTCCCA CAGCGGCGCA GGTAGACGGG GCCGACCTGG CCTCTCCAAT GTCTCCTCGA 
ACGAGCAAAA GCCGCATGTC CATGAAGCTA CGTCGCTCCT CTGGCTCCGC CAATAAGTCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014957495.1
CDS	457..6759
Translation	MFSKKPPGTGKKSTQKVLDTKKDALTRLKHLRIVIGRIFTAEPPEKPREHENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILSSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_015102009.1
Organism	Ovis aries(sheep)
Definition	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015102009.1

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCC GGGGACAGGG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCCCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAACGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTAACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GATATTGATG ATGCTCAAAT ACTTCCTCGT 
CCAACTAGAG TCAGACATTT TTCGCAAAGT GAAGACACTG GAAATGAAGT TTTTGGTGCT 
TTGAGTGAGG AGCAGCCATT GCCTCGAAGT AGCAGCACTT CTGACATCTT GGAACCATTC 
ACTGTTGAAC GAGCCAAAGT CAATAAAGAG GACATGAGCA CAAAACTGCC TCCTCTTAAT 
AGTGATATTG GCAGCAGCAA TGCTAATGTT CCTGATCTGA TGGATGAGTT TATAGCAGAA 
CGACTTCGAA GTGGTAATGC CTCAACTATG ACACGAAGAG GAAGTAGTCC AGGTAGCCTC 
GAAATTCCCA AAGACCTCCC TGATATTCTA AATAAGCAAA ACCAAATGCG TCCTATTGAT 
GACCCAGGTG TACCCTCAGA ATGGACTTCT CCTGCCAGTG CAGGGAGCAG TGATCTTATC 
AGCTCAGATA GTCATTCGGA TTCTTTCAGC GCTTTCCAGT ATGATGGCCG AAAATTTGAC 
AATTTTGGTT TTGGAGCTGA TACTGGGAAT GCATCCTCTG CTGACATGGA TTCAGGTTCT 
GGCCATTATC AGAGTGCTGA AGAGCAAGAG GTGGCTAGTC TAACCACACT ACACATTGAC 
TCAGAAACAA GCAGTCTTAA TCAACAAGCT TTCTGTACTG AAGTTGCAAC GGTTACTGGT 
TCAGAAAGTG CCTCTCCAGT CCATTCAGCT CTGGGATCCA GGTCACAGAC ACCTTCTCCT 
TCCACACTGA ATATAGATCA CATGGAACAG AAGGATCTAC AGCTCGATGA GAAGCTCCAC 
CACTCTGTTC TTCAGACACC AGATGACCTA GAAATCAGTG AATTTCCATC TGAATGCTGT 
AGTGTGATGG CAGGGGGCAC TCTTACAGGA TGGCATGCTG ATGTTGCTAC TGTAATGTGG 
CGAAGGATGC TGGGTATTTT GGGAGATGTC AACGCTATTA TGGATCCTGA AATACATGCT 
CAAGTTTTTG ATTACCTCTG TGAACTTTGG CAGAATCTAG CTAAGATTAG AGATAATCTT 
GGCATTTCAA CTGATAACCT GACCTCCCCT TCTCCACCGG TTTTAATTCC TCCACTGAGA 
ATTCTTACAC CTTGGCTTTT TAAGGCAACC ATGTTAACTG ATAAATATAA ACAAGGTAAA 
TTACATGCCT ATAAACTTAT TTGTAATACA ATGAAAAGAA GACAAGATGT TTCTCCAAAT 
AGAGATTTTC TAACACATTT CTACAACATA ATGCATTGTG GATTGCTTCA CATTGATCAG 
GATATTGTCA ATACAATCAT CAAGCACTGC TCACCTCAAT TTTTCTCACT TGGTTTGCCT 
GGTGCAACAA TGCTTATTAT GGATTTTATT GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT 
TTTCTCAATG CACCAAGAGT GGAAGCACAA GTTCTTCTGG GATCTTTGGT TTGCTTTCCC 
AACTTGTATT GTGAACTGCC TGCTCTCCAT CCCAACATCC CTGATATTGC TGTGTCTCAA 
TTTACAGATG TTAAGGAACT TATAATCAAA ACTGTGTTAA GCTCTGCAAG AGATGAGCCC 
TCTGGTCCTG CACGATGTGT AGCACTTTGC AGTTTAGGTA TTTGGATTTG TGAAGAACTA 
GTCCATGAGT CTCATCATCC TCAAATTAAG GAAGCTCTGA ATGTAATTTG TGTTTCCTTA 
AAGTTTACTA ATAAAACAGT AGCCCATGTA GCTTGTAACA TGCTTCACAT GCTGGTTCAT 
TATGTACCTA GACTTCAGAT TTACCAGCCT GATTCTCCCT TGAAAATTAT TCAAATTCTA 
ATAGCCACTA TTACCCATCT TTTGCCAAGT ACGGAAGCGT CATCTTATGA AATGGACAAG 
AGGTTGGTGG TATCCTTACT TCTGTGTCTT CTGGACTGGA TCATGGCCTT ACCTCTTAAG 
ACACTGCTCC AACCAGTCCA TGCTACAGGA GCAGAAAATG ATAAAATAGA AAAATCTGTT 
CTTAACTGCA TTTATAAGGT ATTACATGGG TGTGTTTATG GAGCTCAGTG TTTTAGCAAT 
CCGAGGTATT TTCCAATAAG CCTTTCTGAT TTGGCATCTG TAGATTATGA TCCTTTTATG 
CATTTGGAAA GCCTAAAAGA GCCTGAACCT TTGCACTCTC CTGATTCGGA ACGATCTTCT 
AAACTCCAGC CAGTAACAGA AGTGAAAACT CAGATGCAGC AAGGATTAAT CTCCATAGCT 
GCCCGCACTG TTATCACACA TCTGGTGAAT CACTTGGGCC ATTATCCAAT GAGCGGTGGT 
CCTGCTATGT TAACAAGTCA GGTGTGTGAG AATCACGACA ATCATTACAG TGAAAGCACT 
GAACTTTCCC CTGAACTCTT CGAGAGTCCA AATATTCAGT TTTTTGTGTT AAACAATACA 
ACCTTAGTGT CCTGTATCCA GATCAGAGCT GAAGAGAGTA TACCTGGAGG AGGTTTATCT 
GCTGGTCTTG CATCAGCCAA TTCAAATGTC AGAATTATAG TACGTGATCT TTCTGGAAAA 
TATTCATGGG ATTCTGCCAT CCTGTATGGA CCACCTCTTG TAAGTGGTCT GTCAGAACCT 
ACATCTCTCG TACTTTCATT GTCTCACCAA GAGAAGCCAG AAGAACCTTC TGCATCTAAT 
GAATGCTTAG AAGATATAAC AGTTAAAGAT GGGATTTCCC TCCAGTTTAA AAGAAGTTTT 
AGAGAAACTG TACCAACTTG GGACACAATC AGAGATGAAG AAGATGTTCT TGATGAGCTT 
TTGCAGTATT TAGGTGTAAC CAGTCCTGAA TGCTTACAGA GAACTGGAGT CTCACTTAAT 
ATTCCTGCTC CACAGCCTGT GTGCATTTCT GAAAAACAGG AGAATGATGT TATTAATGCT 
ATCCTTAAGC AGCATACAGA GGAAAAAGAA TTTGTTGAGA AACACTTTAA TGACTTAAAC 
ATGAAAGCTG CAGAACAAGA TGAACCAACA CCTCAGAAAC CTCAGTCAGC ATTTTATTAT 
TGCAGATTGC TTCTTAGTAT ATTGGGAATG AATTCCTGGG ACAAAAGGAG GAGCTTTCAT 
CTCCTGAAGA AAAATGAAAA ACTACTTAGA GAACTTAGGA ACTTGGACTC AAGACAGTGC 
CGAGAGACAC ACAAGATTGC AGTATTTTAT GTTGCTGAAG GACAAGAAGA CAAACATTCC 
ATTCTCACCA ATACAGGAGG AAGTCAAGCA TATGAAGATT TTGTAGCTGG TCTTGGTTGG 
GAGGTAAATC TTACAAACCA TTGTGGTTTC ATGGGAGGAC TACAAAAAAA TAAAAGCACT 
GGACTGACCA CACCGTATTT TGCTACCTCT ACAGTTGAAG TAATATTTCA TGTGTCAACA 
AGAATGCCTT CAGATTCTGA TGACTCTTTG ACCAAAAAAT TGAGACATTT AGGAAATGAT 
GAAGTGCACA TTGTTTGGTC AGAGCATACT AGAGACTACA GGAGAGGAAT TATTCCTACA 
GAATTTGGTG ATGTCCTTAT TGTTATATAC CCGATGAAAA ACCACATGTT CAGTATCCAG 
ATAATGAAAA AACCAGAGGT TCCCTTCTTT GGTCCACTCT TTGATGGTGC TATTGTGAAT 
GGAAAGGTCC TACCTATTAT GGTTCGAGCA ACAGCTATAA ATGCAAGCCG TGCTCTGAAA 
TCCCTGATTC CATTGTATCA AAACTTCTAT GAGGAGAGAG CACGATATCT GCAAACGATT 
GTCCAGCACC ATTTAGAGCC AACGACATTT GAAGATTTTG CAGCACAGGT TTTTTCTCCA 
GCTCCCTACC ATCATTTACC ATCTGATGCC GTGGGCTCCT ACCCAGAGAT CCTATCCAGT 
GAAACTCCCA CAGCGGCGCA GGTAGACGGG GCCGACCTGG CCTCTCCAAT GTCTCCTCGA 
ACGAGCAAAA GCCGCATGTC CATGAAGCTA CGTCGCTCCT CTGGCTCCGC CAATAAGTCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_012098811.3 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011954201.2 Ovis aries Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X1, mRNA.

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CloneID	OSm67921
Clone ID Related Accession (Same CDS sequence)	XM_012098811.3
Accession Version	XM_012098811.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	ral GTPase-activating protein subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040269.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_012098811.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCGA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCCCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TAACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTGTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCGCAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AATAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAATATACA ATTTCAAAAA GAACCAAACT CTACTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTACG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TACCACACTA 
GCTGAAAAGG TGAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCA 
TCAGCAAGCA GTGAAACAAT TAAGGAAACG ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC TCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAA CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTCCC TGATATTCTA 
AATAAGCAAA ACCAAATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAGT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCTGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATTGAC TCAGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG AAGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTGA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA GAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTCTG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAACATA 
ATGCATTGTG GATTGCTTCA CATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCAACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAACTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCACAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACGGAAGCGT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTT CTTAACTGCA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCTTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAGATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATCACACA TCTGGTGAAT 
CACTTGGGCC ATTATCCAAT GAGCGGTGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CTGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT GTCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATC 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GAACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTTAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACA 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCCTGGG ACAAAAGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGACTGACCA CACCGTATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA TGTGTCAACA AGAATGCCTT CAGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTTATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCCCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGATATCT GCAAACGATT GTCCAGCACC ATTTAGAGCC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GTGGGCTCCT ACCCAGAGAT CCTATCCAGT GAAACTCCCA CAGCGGCGCA GGTAGACGGG 
GCCGACCTGG CCTCTCCAAT GTCTCCTCGA ACGAGCAAAA GCCGCATGTC CATGAAGCTG 
CGTCGCTCCT CTGGCTCCGC CAATAAGTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011954201.2
CDS	88..7860
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQENKSENTTSFVGTPENIQFQKEPNSTVFTNISAPNQLDSFTKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDTTLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILSSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_012098811.3
Organism	Ovis aries(sheep)
Definition	Ovis aries Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012098811.3

ORF Insert Sequence:

ATGTTCTCGA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCCCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TAACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTGTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCGCAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AATAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAATATACA ATTTCAAAAA GAACCAAACT CTACTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTACG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TACCACACTA 
GCTGAAAAGG TGAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCA 
TCAGCAAGCA GTGAAACAAT TAAGGAAACG ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC TCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAA CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTCCC TGATATTCTA 
AATAAGCAAA ACCAAATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAGT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCTGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATTGAC TCAGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG AAGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTGA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA GAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTCTG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAACATA 
ATGCATTGTG GATTGCTTCA CATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCAACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAACTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCACAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACGGAAGCGT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTT CTTAACTGCA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCTTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAGATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATCACACA TCTGGTGAAT 
CACTTGGGCC ATTATCCAAT GAGCGGTGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CTGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT GTCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATC 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GAACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTTAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACA 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCCTGGG ACAAAAGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGACTGACCA CACCGTATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA TGTGTCAACA AGAATGCCTT CAGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTTATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCCCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGATATCT GCAAACGATT GTCCAGCACC ATTTAGAGCC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GTGGGCTCCT ACCCAGAGAT CCTATCCAGT GAAACTCCCA CAGCGGCGCA GGTAGACGGG 
GCCGACCTGG CCTCTCCAAT GTCTCCTCGA ACGAGCAAAA GCCGCATGTC CATGAAGCTG 
CGTCGCTCCT CTGGCTCCGC CAATAAGTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_027957249.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027813050.1 Ovis aries Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X2, mRNA.

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CloneID	OSm67922
Clone ID Related Accession (Same CDS sequence)	XM_027957249.1
Accession Version	XM_027957249.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	ral GTPase-activating protein subunit alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040269.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCGA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCCCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TAACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGGTGCAG TTCCTGTCAT TGACAGTTCA 
TCCCGTCATG CACCAAGCTT GCAGAGTTCC ACAGAGGCTT CTTCAATAAC TAGATCCACT 
GAGAGCCACA TCACTGATAC TCATAGTAGA GAGTCTTCTC TGGAAGTTGG TGATAGCATA 
TATGACCATC TCTGTCACTT AATAGATCCA GTAGAACTTG CAGATTCAGC TTTTGAACAA 
ATCCAGTACA TTGACCTTGA AGGAGATGAT GATCTTCTTT CCTCCCTGAA AGAATATTTT 
AAGGAAACCC AAGAAAGTCA CAGTAAAAAC GAGTTAGATA AAGATGCAGC TTCGCAGGAA 
GTGATCTCTG TTGCAGTAAA TAAGGATGAA AGATCATCCT CAGATAAATT AGAGTACATA 
GACCAGGAAA ATAAATCAGA AAATACCACC TCTTTTGTTG GAACTCCTGA AAATATACAA 
TTTCAAAAAG AACCAAACTC TACTGTCTTC ACAAATATTA GTGCACCCAA CCAGTTAGAC 
AGCTTTACGA AAACAAAAAC TTCTGAAAAA TGTAAACAAC TAAACAATGA TAAACAGTCA 
TCGGAACCTA GTTCAGGTAG CCCTTGTGAT AAAGAAAAGA GAAAACATCT GTATAGACAA 
ACAGCCACAG AATTAGATGC CTGTATAGAT ACCACACTAG CTGAAAAGGT GAAGGGTGTG 
CAAATTAATG AAAAAATCAC TGTCCCACAT GTTTTGCATG CCAAGAAAAC TACACTAAAA 
GCACCTGTTA ACCGTAGAAT GCCTCATGTT ACAAGCACTA GCAAGCTTTC TCCAACTAAA 
AGGAGCTTGT CTGAAACAGT AACTCACAGA GCCAAAATCA TGAAAATTGC TACTAAAAAG 
CGAAATAGTG TTCATGTTAC TTTTAGACCT TCTACTGAGT CAGTTCAGTT TTATAATCCT 
CTGGAAAACA AAGAGGCTCC ATGGAAGATG AGACTGCGGA AGCTCAGTGG CTTTAGTGGT 
AGTAATAGCA ACATCAGCAA CCCCCACACC AGCTCCAACA GTGCTGCAGA GCTAGTAAAA 
CCTGGTGTGT ACAGGCCTCT AGATACAATT AGTGCAGCAT CAGCAAGCAG TGAAACAATT 
AAGGAAACGA CAGAGATTCC CACCGCCATA TTACAGAAAG AAGGAATTGC AACTAATCAG 
TTAGGTAGTC GTAGTACTAT TAAGTCATCA AGTCACGAGG CAGGACTACC GCAGGGCTCC 
CTGGGTGGCG TTTATAAAAC TGTTGTCCAT GCTCTTTCGA AGCCGAAGGC AAATGTTTCC 
CCACAGAGGC AGAACAGAAT GCCACCAGAG GCTCCACTGA GGGATCTGTA CAGTCATGTA 
ATGGGCTATT TTGGAAGGAA AGCTGCAGTC AATAAAGAGG ACATGAGCAC AAAACTGCCT 
CCTCTTAATA GTGATATTGG CAGCAGCAAT GCTAATGTTC CTGATCTGAT GGATGAGTTT 
ATAGCAGAAC GACTTCGAAG TGGTAATGCC TCAACTATGA CACGAAGAGG AAGTAGTCCA 
GGTAGCCTCG AAATTCCCAA AGACCTCCCT GATATTCTAA ATAAGCAAAA CCAAATGCGT 
CCTATTGATG ACCCAGGTGT ACCCTCAGAA TGGACTTCTC CTGCCAGTGC AGGGAGCAGT 
GATCTTATCA GCTCAGATAG TCATTCGGAT TCTTTCAGCG CTTTCCAGTA TGATGGCCGA 
AAATTTGACA ATTTTGGTTT TGGAGCTGAT ACTGGGAATG CATCCTCTGC TGACATGGAT 
TCAGGTTCTG GCCATTATCA GAGTGCTGAA GAGCAAGAGG TGGCTAGTCT AACCACACTA 
CACATTGACT CAGAAACAAG CAGTCTTAAT CAACAAGCTT TCTGTACTGA AGTTGCAACG 
GTTACTGGTT CAGAAAGTGC CTCTCCAGTC CATTCAGCTC TGGGATCCAG GTCACAGACA 
CCTTCTCCTT CCACACTGAA TATAGATCAC ATGGAACAGA AGGATCTACA GCTCGATGAG 
AAGCTCCACC ACTCTGTTCT TCAGACACCA GATGACCTAG AAATCAGTGA ATTTCCATCT 
GAATGCTGTA GTGTGATGGC AGGGGGCACT CTTACAGGAT GGCATGCTGA TGTTGCTACT 
GTAATGTGGC GAAGGATGCT GGGTATTTTG GGAGATGTCA ACGCTATTAT GGATCCTGAA 
ATACATGCTC AAGTTTTTGA TTACCTCTGT GAACTTTGGC AGAATCTAGC TAAGATTAGA 
GATAATCTTG GCATTTCAAC TGATAACCTG ACCTCCCCTT CTCCACCGGT TTTAATTCCT 
CCACTGAGAA TTCTTACACC TTGGCTTTTT AAGGCAACCA TGTTAACTGA TAAATATAAA 
CAAGGTAAAT TACATGCCTA TAAACTTATT TGTAATACAA TGAAAAGAAG ACAAGATGTT 
TCTCCAAATA GAGATTTTCT AACACATTTC TACAACATAA TGCATTGTGG ATTGCTTCAC 
ATTGATCAGG ATATTGTCAA TACAATCATC AAGCACTGCT CACCTCAATT TTTCTCACTT 
GGTTTGCCTG GTGCAACAAT GCTTATTATG GATTTTATTG TAGCAGCTGG TAGAGTGGCT 
TCTTCAGCTT TTCTCAATGC ACCAAGAGTG GAAGCACAAG TTCTTCTGGG ATCTTTGGTT 
TGCTTTCCCA ACTTGTATTG TGAACTGCCT GCTCTCCATC CCAACATCCC TGATATTGCT 
GTGTCTCAAT TTACAGATGT TAAGGAACTT ATAATCAAAA CTGTGTTAAG CTCTGCAAGA 
GATGAGCCCT CTGGTCCTGC ACGATGTGTA GCACTTTGCA GTTTAGGTAT TTGGATTTGT 
GAAGAACTAG TCCATGAGTC TCATCATCCT CAAATTAAGG AAGCTCTGAA TGTAATTTGT 
GTTTCCTTAA AGTTTACTAA TAAAACAGTA GCCCATGTAG CTTGTAACAT GCTTCACATG 
CTGGTTCATT ATGTACCTAG ACTTCAGATT TACCAGCCTG ATTCTCCCTT GAAAATTATT 
CAAATTCTAA TAGCCACTAT TACCCATCTT TTGCCAAGTA CGGAAGCGTC ATCTTATGAA 
ATGGACAAGA GGTTGGTGGT ATCCTTACTT CTGTGTCTTC TGGACTGGAT CATGGCCTTA 
CCTCTTAAGA CACTGCTCCA ACCAGTCCAT GCTACAGGAG CAGAAAATGA TAAAATAGAA 
AAATCTGTTC TTAACTGCAT TTATAAGGTA TTACATGGGT GTGTTTATGG AGCTCAGTGT 
TTTAGCAATC CGAGGTATTT TCCAATAAGC CTTTCTGATT TGGCATCTGT AGATTATGAT 
CCTTTTATGC ATTTGGAAAG CCTAAAAGAG CCTGAACCTT TGCACTCTCC TGATTCGGAA 
CGATCTTCTA AACTCCAGCC AGTAACAGAA GTGAAAACTC AGATGCAGCA AGGATTAATC 
TCCATAGCTG CCCGCACTGT TATCACACAT CTGGTGAATC ACTTGGGCCA TTATCCAATG 
AGCGGTGGTC CTGCTATGTT AACAAGTCAG GTGTGTGAGA ATCACGACAA TCATTACAGT 
GAAAGCACTG AACTTTCCCC TGAACTCTTC GAGAGTCCAA ATATTCAGTT TTTTGTGTTA 
AACAATACAA CCTTAGTGTC CTGTATCCAG ATCAGAGCTG AAGAGAGTAT ACCTGGAGGA 
GGTTTATCTG CTGGTCTTGC ATCAGCCAAT TCAAATGTCA GAATTATAGT ACGTGATCTT 
TCTGGAAAAT ATTCATGGGA TTCTGCCATC CTGTATGGAC CACCTCTTGT AAGTGGTCTG 
TCAGAACCTA CATCTCTCGT ACTTTCATTG TCTCACCAAG AGAAGCCAGA AGAACCTTCT 
GCATCTAATG AATGCTTAGA AGATATAACA GTTAAAGATG GGATTTCCCT CCAGTTTAAA 
AGAAGTTTTA GAGAAACTGT ACCAACTTGG GACACAATCA GAGATGAAGA AGATGTTCTT 
GATGAGCTTT TGCAGTATTT AGGTGTAACC AGTCCTGAAT GCTTACAGAG AACTGGAGTC 
TCACTTAATA TTCCTGCTCC ACAGCCTGTG TGCATTTCTG AAAAACAGGA GAATGATGTT 
ATTAATGCTA TCCTTAAGCA GCATACAGAG GAAAAAGAAT TTGTTGAGAA ACACTTTAAT 
GACTTAAACA TGAAAGCTGC AGAACAAGAT GAACCAACAC CTCAGAAACC TCAGTCAGCA 
TTTTATTATT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCCTGGGA CAAAAGGAGG 
AGCTTTCATC TCCTGAAGAA AAATGAAAAA CTACTTAGAG AACTTAGGAA CTTGGACTCA 
AGACAGTGCC GAGAGACACA CAAGATTGCA GTATTTTATG TTGCTGAAGG ACAAGAAGAC 
AAACATTCCA TTCTCACCAA TACAGGAGGA AGTCAAGCAT ATGAAGATTT TGTAGCTGGT 
CTTGGTTGGG AGGTAAATCT TACAAACCAT TGTGGTTTCA TGGGAGGACT ACAAAAAAAT 
AAAAGCACTG GACTGACCAC ACCGTATTTT GCTACCTCTA CAGTTGAAGT AATATTTCAT 
GTGTCAACAA GAATGCCTTC AGATTCTGAT GACTCTTTGA CCAAAAAATT GAGACATTTA 
GGAAATGATG AAGTGCACAT TGTTTGGTCA GAGCATACTA GAGACTACAG GAGAGGAATT 
ATTCCTACAG AATTTGGTGA TGTCCTTATT GTTATATACC CGATGAAAAA CCACATGTTC 
AGTATCCAGA TAATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCACTCTT TGATGGTGCT 
ATTGTGAATG GAAAGGTCCT ACCTATTATG GTTCGAGCAA CAGCTATAAA TGCAAGCCGT 
GCTCTGAAAT CCCTGATTCC ATTGTATCAA AACTTCTATG AGGAGAGAGC ACGATATCTG 
CAAACGATTG TCCAGCACCA TTTAGAGCCA ACGACATTTG AAGATTTTGC AGCACAGGTT 
TTTTCTCCAG CTCCCTACCA TCATTTACCA TCTGATGCCG TGGGCTCCTA CCCAGAGATC 
CTATCCAGTG AAACTCCCAC AGCGGCGCAG GTAGACGGGG CCGACCTGGC CTCTCCAATG 
TCTCCTCGAA CGAGCAAAAG CCGCATGTCC ATGAAGCTGC GTCGCTCCTC TGGCTCCGCC 
AATAAGTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027813050.1
CDS	87..7718
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQENKSENTTSFVGTPENIQFQKEPNSTVFTNISAPNQLDSFTKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDTTLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILSSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_027957249.1
Organism	Ovis aries(sheep)
Definition	Ovis aries Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027957249.1

ORF Insert Sequence:

ATGTTCTCGA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCCCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TAACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGGTGCAG TTCCTGTCAT TGACAGTTCA 
TCCCGTCATG CACCAAGCTT GCAGAGTTCC ACAGAGGCTT CTTCAATAAC TAGATCCACT 
GAGAGCCACA TCACTGATAC TCATAGTAGA GAGTCTTCTC TGGAAGTTGG TGATAGCATA 
TATGACCATC TCTGTCACTT AATAGATCCA GTAGAACTTG CAGATTCAGC TTTTGAACAA 
ATCCAGTACA TTGACCTTGA AGGAGATGAT GATCTTCTTT CCTCCCTGAA AGAATATTTT 
AAGGAAACCC AAGAAAGTCA CAGTAAAAAC GAGTTAGATA AAGATGCAGC TTCGCAGGAA 
GTGATCTCTG TTGCAGTAAA TAAGGATGAA AGATCATCCT CAGATAAATT AGAGTACATA 
GACCAGGAAA ATAAATCAGA AAATACCACC TCTTTTGTTG GAACTCCTGA AAATATACAA 
TTTCAAAAAG AACCAAACTC TACTGTCTTC ACAAATATTA GTGCACCCAA CCAGTTAGAC 
AGCTTTACGA AAACAAAAAC TTCTGAAAAA TGTAAACAAC TAAACAATGA TAAACAGTCA 
TCGGAACCTA GTTCAGGTAG CCCTTGTGAT AAAGAAAAGA GAAAACATCT GTATAGACAA 
ACAGCCACAG AATTAGATGC CTGTATAGAT ACCACACTAG CTGAAAAGGT GAAGGGTGTG 
CAAATTAATG AAAAAATCAC TGTCCCACAT GTTTTGCATG CCAAGAAAAC TACACTAAAA 
GCACCTGTTA ACCGTAGAAT GCCTCATGTT ACAAGCACTA GCAAGCTTTC TCCAACTAAA 
AGGAGCTTGT CTGAAACAGT AACTCACAGA GCCAAAATCA TGAAAATTGC TACTAAAAAG 
CGAAATAGTG TTCATGTTAC TTTTAGACCT TCTACTGAGT CAGTTCAGTT TTATAATCCT 
CTGGAAAACA AAGAGGCTCC ATGGAAGATG AGACTGCGGA AGCTCAGTGG CTTTAGTGGT 
AGTAATAGCA ACATCAGCAA CCCCCACACC AGCTCCAACA GTGCTGCAGA GCTAGTAAAA 
CCTGGTGTGT ACAGGCCTCT AGATACAATT AGTGCAGCAT CAGCAAGCAG TGAAACAATT 
AAGGAAACGA CAGAGATTCC CACCGCCATA TTACAGAAAG AAGGAATTGC AACTAATCAG 
TTAGGTAGTC GTAGTACTAT TAAGTCATCA AGTCACGAGG CAGGACTACC GCAGGGCTCC 
CTGGGTGGCG TTTATAAAAC TGTTGTCCAT GCTCTTTCGA AGCCGAAGGC AAATGTTTCC 
CCACAGAGGC AGAACAGAAT GCCACCAGAG GCTCCACTGA GGGATCTGTA CAGTCATGTA 
ATGGGCTATT TTGGAAGGAA AGCTGCAGTC AATAAAGAGG ACATGAGCAC AAAACTGCCT 
CCTCTTAATA GTGATATTGG CAGCAGCAAT GCTAATGTTC CTGATCTGAT GGATGAGTTT 
ATAGCAGAAC GACTTCGAAG TGGTAATGCC TCAACTATGA CACGAAGAGG AAGTAGTCCA 
GGTAGCCTCG AAATTCCCAA AGACCTCCCT GATATTCTAA ATAAGCAAAA CCAAATGCGT 
CCTATTGATG ACCCAGGTGT ACCCTCAGAA TGGACTTCTC CTGCCAGTGC AGGGAGCAGT 
GATCTTATCA GCTCAGATAG TCATTCGGAT TCTTTCAGCG CTTTCCAGTA TGATGGCCGA 
AAATTTGACA ATTTTGGTTT TGGAGCTGAT ACTGGGAATG CATCCTCTGC TGACATGGAT 
TCAGGTTCTG GCCATTATCA GAGTGCTGAA GAGCAAGAGG TGGCTAGTCT AACCACACTA 
CACATTGACT CAGAAACAAG CAGTCTTAAT CAACAAGCTT TCTGTACTGA AGTTGCAACG 
GTTACTGGTT CAGAAAGTGC CTCTCCAGTC CATTCAGCTC TGGGATCCAG GTCACAGACA 
CCTTCTCCTT CCACACTGAA TATAGATCAC ATGGAACAGA AGGATCTACA GCTCGATGAG 
AAGCTCCACC ACTCTGTTCT TCAGACACCA GATGACCTAG AAATCAGTGA ATTTCCATCT 
GAATGCTGTA GTGTGATGGC AGGGGGCACT CTTACAGGAT GGCATGCTGA TGTTGCTACT 
GTAATGTGGC GAAGGATGCT GGGTATTTTG GGAGATGTCA ACGCTATTAT GGATCCTGAA 
ATACATGCTC AAGTTTTTGA TTACCTCTGT GAACTTTGGC AGAATCTAGC TAAGATTAGA 
GATAATCTTG GCATTTCAAC TGATAACCTG ACCTCCCCTT CTCCACCGGT TTTAATTCCT 
CCACTGAGAA TTCTTACACC TTGGCTTTTT AAGGCAACCA TGTTAACTGA TAAATATAAA 
CAAGGTAAAT TACATGCCTA TAAACTTATT TGTAATACAA TGAAAAGAAG ACAAGATGTT 
TCTCCAAATA GAGATTTTCT AACACATTTC TACAACATAA TGCATTGTGG ATTGCTTCAC 
ATTGATCAGG ATATTGTCAA TACAATCATC AAGCACTGCT CACCTCAATT TTTCTCACTT 
GGTTTGCCTG GTGCAACAAT GCTTATTATG GATTTTATTG TAGCAGCTGG TAGAGTGGCT 
TCTTCAGCTT TTCTCAATGC ACCAAGAGTG GAAGCACAAG TTCTTCTGGG ATCTTTGGTT 
TGCTTTCCCA ACTTGTATTG TGAACTGCCT GCTCTCCATC CCAACATCCC TGATATTGCT 
GTGTCTCAAT TTACAGATGT TAAGGAACTT ATAATCAAAA CTGTGTTAAG CTCTGCAAGA 
GATGAGCCCT CTGGTCCTGC ACGATGTGTA GCACTTTGCA GTTTAGGTAT TTGGATTTGT 
GAAGAACTAG TCCATGAGTC TCATCATCCT CAAATTAAGG AAGCTCTGAA TGTAATTTGT 
GTTTCCTTAA AGTTTACTAA TAAAACAGTA GCCCATGTAG CTTGTAACAT GCTTCACATG 
CTGGTTCATT ATGTACCTAG ACTTCAGATT TACCAGCCTG ATTCTCCCTT GAAAATTATT 
CAAATTCTAA TAGCCACTAT TACCCATCTT TTGCCAAGTA CGGAAGCGTC ATCTTATGAA 
ATGGACAAGA GGTTGGTGGT ATCCTTACTT CTGTGTCTTC TGGACTGGAT CATGGCCTTA 
CCTCTTAAGA CACTGCTCCA ACCAGTCCAT GCTACAGGAG CAGAAAATGA TAAAATAGAA 
AAATCTGTTC TTAACTGCAT TTATAAGGTA TTACATGGGT GTGTTTATGG AGCTCAGTGT 
TTTAGCAATC CGAGGTATTT TCCAATAAGC CTTTCTGATT TGGCATCTGT AGATTATGAT 
CCTTTTATGC ATTTGGAAAG CCTAAAAGAG CCTGAACCTT TGCACTCTCC TGATTCGGAA 
CGATCTTCTA AACTCCAGCC AGTAACAGAA GTGAAAACTC AGATGCAGCA AGGATTAATC 
TCCATAGCTG CCCGCACTGT TATCACACAT CTGGTGAATC ACTTGGGCCA TTATCCAATG 
AGCGGTGGTC CTGCTATGTT AACAAGTCAG GTGTGTGAGA ATCACGACAA TCATTACAGT 
GAAAGCACTG AACTTTCCCC TGAACTCTTC GAGAGTCCAA ATATTCAGTT TTTTGTGTTA 
AACAATACAA CCTTAGTGTC CTGTATCCAG ATCAGAGCTG AAGAGAGTAT ACCTGGAGGA 
GGTTTATCTG CTGGTCTTGC ATCAGCCAAT TCAAATGTCA GAATTATAGT ACGTGATCTT 
TCTGGAAAAT ATTCATGGGA TTCTGCCATC CTGTATGGAC CACCTCTTGT AAGTGGTCTG 
TCAGAACCTA CATCTCTCGT ACTTTCATTG TCTCACCAAG AGAAGCCAGA AGAACCTTCT 
GCATCTAATG AATGCTTAGA AGATATAACA GTTAAAGATG GGATTTCCCT CCAGTTTAAA 
AGAAGTTTTA GAGAAACTGT ACCAACTTGG GACACAATCA GAGATGAAGA AGATGTTCTT 
GATGAGCTTT TGCAGTATTT AGGTGTAACC AGTCCTGAAT GCTTACAGAG AACTGGAGTC 
TCACTTAATA TTCCTGCTCC ACAGCCTGTG TGCATTTCTG AAAAACAGGA GAATGATGTT 
ATTAATGCTA TCCTTAAGCA GCATACAGAG GAAAAAGAAT TTGTTGAGAA ACACTTTAAT 
GACTTAAACA TGAAAGCTGC AGAACAAGAT GAACCAACAC CTCAGAAACC TCAGTCAGCA 
TTTTATTATT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCCTGGGA CAAAAGGAGG 
AGCTTTCATC TCCTGAAGAA AAATGAAAAA CTACTTAGAG AACTTAGGAA CTTGGACTCA 
AGACAGTGCC GAGAGACACA CAAGATTGCA GTATTTTATG TTGCTGAAGG ACAAGAAGAC 
AAACATTCCA TTCTCACCAA TACAGGAGGA AGTCAAGCAT ATGAAGATTT TGTAGCTGGT 
CTTGGTTGGG AGGTAAATCT TACAAACCAT TGTGGTTTCA TGGGAGGACT ACAAAAAAAT 
AAAAGCACTG GACTGACCAC ACCGTATTTT GCTACCTCTA CAGTTGAAGT AATATTTCAT 
GTGTCAACAA GAATGCCTTC AGATTCTGAT GACTCTTTGA CCAAAAAATT GAGACATTTA 
GGAAATGATG AAGTGCACAT TGTTTGGTCA GAGCATACTA GAGACTACAG GAGAGGAATT 
ATTCCTACAG AATTTGGTGA TGTCCTTATT GTTATATACC CGATGAAAAA CCACATGTTC 
AGTATCCAGA TAATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCACTCTT TGATGGTGCT 
ATTGTGAATG GAAAGGTCCT ACCTATTATG GTTCGAGCAA CAGCTATAAA TGCAAGCCGT 
GCTCTGAAAT CCCTGATTCC ATTGTATCAA AACTTCTATG AGGAGAGAGC ACGATATCTG 
CAAACGATTG TCCAGCACCA TTTAGAGCCA ACGACATTTG AAGATTTTGC AGCACAGGTT 
TTTTCTCCAG CTCCCTACCA TCATTTACCA TCTGATGCCG TGGGCTCCTA CCCAGAGATC 
CTATCCAGTG AAACTCCCAC AGCGGCGCAG GTAGACGGGG CCGACCTGGC CTCTCCAATG 
TCTCCTCGAA CGAGCAAAAG CCGCATGTCC ATGAAGCTGC GTCGCTCCTC TGGCTCCGCC 
AATAAGTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_027957251.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027813052.1 Ovis aries Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X4, mRNA.

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CloneID	OSm67923
Clone ID Related Accession (Same CDS sequence)	XM_027957251.1 , XM_027957250.1
Accession Version	XM_027957251.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	ral GTPase-activating protein subunit alpha-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040269.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCGA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCCCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TAACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTGTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCGCAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AATAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAATATACA ATTTCAAAAA GAACCAAACT CTACTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTACG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TACCACACTA 
GCTGAAAAGG TGAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCA 
TCAGCAAGCA GTGAAACAAT TAAGGAAACG ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC TCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAA CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTCCC TGATATTCTA 
AATAAGCAAA ACCAAATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAGT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCTGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATTGAC TCAGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG AAGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTGA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA GAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTCTG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAACATA 
ATGCATTGTG GATTGCTTCA CATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCAACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAACTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCACAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACGGAAGCGT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTT CTTAACTGCA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCTTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAGATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATCACACA TCTGGTGAAT 
CACTTGGGCC ATTATCCAAT GAGCGGTGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CTGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT GTCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATC 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GAACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTTAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACA 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCCTGGG ACAAAAGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGACTGACCA CACCGTATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA TGTGTCAACA AGAATGCCTT CAGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTTATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCCCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGATATCT GCAAACGATT GTCCAGCACC ATTTAGAGCC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GATCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027813052.1
CDS	90..7718
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQENKSENTTSFVGTPENIQFQKEPNSTVFTNISAPNQLDSFTKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDTTLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDADH

Target ORF information:

RefSeq Version	XM_027957251.1
Organism	Ovis aries(sheep)
Definition	Ovis aries Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027957251.1

ORF Insert Sequence:

ATGTTCTCGA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCCCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TAACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTGTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCGCAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AATAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAATATACA ATTTCAAAAA GAACCAAACT CTACTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTACG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TACCACACTA 
GCTGAAAAGG TGAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCA 
TCAGCAAGCA GTGAAACAAT TAAGGAAACG ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC TCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAA CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTCCC TGATATTCTA 
AATAAGCAAA ACCAAATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAGT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCTGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATTGAC TCAGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG AAGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTGA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA GAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTCTG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAACATA 
ATGCATTGTG GATTGCTTCA CATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCAACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAACTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCACAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACGGAAGCGT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTT CTTAACTGCA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCTTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAGATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATCACACA TCTGGTGAAT 
CACTTGGGCC ATTATCCAAT GAGCGGTGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CTGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT GTCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATC 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GAACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTTAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACA 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCCTGGG ACAAAAGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGACTGACCA CACCGTATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA TGTGTCAACA AGAATGCCTT CAGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTTATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCCCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGATATCT GCAAACGATT GTCCAGCACC ATTTAGAGCC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GATCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_027957250.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027813051.1 Ovis aries Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X3, mRNA.

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CloneID	OSm67923
Clone ID Related Accession (Same CDS sequence)	XM_027957251.1 , XM_027957250.1
Accession Version	XM_027957250.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	ral GTPase-activating protein subunit alpha-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040269.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCGA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCCCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TAACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTGTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCGCAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AATAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAATATACA ATTTCAAAAA GAACCAAACT CTACTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTACG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TACCACACTA 
GCTGAAAAGG TGAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCA 
TCAGCAAGCA GTGAAACAAT TAAGGAAACG ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC TCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAA CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTCCC TGATATTCTA 
AATAAGCAAA ACCAAATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAGT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCTGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATTGAC TCAGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG AAGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTGA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA GAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTCTG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAACATA 
ATGCATTGTG GATTGCTTCA CATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCAACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAACTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCACAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACGGAAGCGT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTT CTTAACTGCA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCTTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAGATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATCACACA TCTGGTGAAT 
CACTTGGGCC ATTATCCAAT GAGCGGTGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CTGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT GTCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATC 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GAACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTTAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACA 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCCTGGG ACAAAAGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGACTGACCA CACCGTATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA TGTGTCAACA AGAATGCCTT CAGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTTATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCCCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGATATCT GCAAACGATT GTCCAGCACC ATTTAGAGCC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GATCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027813051.1
CDS	90..7718
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQENKSENTTSFVGTPENIQFQKEPNSTVFTNISAPNQLDSFTKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDTTLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDADH

Target ORF information:

RefSeq Version	XM_027957250.1
Organism	Ovis aries(sheep)
Definition	Ovis aries Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027957250.1

ORF Insert Sequence:

ATGTTCTCGA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCCCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TAACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTGTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCGCAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AATAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAATATACA ATTTCAAAAA GAACCAAACT CTACTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTACG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TACCACACTA 
GCTGAAAAGG TGAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCA 
TCAGCAAGCA GTGAAACAAT TAAGGAAACG ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC TCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAA CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTCCC TGATATTCTA 
AATAAGCAAA ACCAAATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAGT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCTGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATTGAC TCAGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG AAGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTGA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA GAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTCTG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAACATA 
ATGCATTGTG GATTGCTTCA CATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCAACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAACTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCACAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACGGAAGCGT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTT CTTAACTGCA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCTTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAGATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATCACACA TCTGGTGAAT 
CACTTGGGCC ATTATCCAAT GAGCGGTGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CTGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT GTCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATC 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GAACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTTAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACA 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCCTGGG ACAAAAGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGACTGACCA CACCGTATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA TGTGTCAACA AGAATGCCTT CAGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTTATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCCCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGATATCT GCAAACGATT GTCCAGCACC ATTTAGAGCC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GATCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_012098820.3 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011954210.2 Ovis aries Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X5, mRNA.

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CloneID	OSm67924
Clone ID Related Accession (Same CDS sequence)	XM_012098820.3
Accession Version	XM_012098820.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	ral GTPase-activating protein subunit alpha-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040269.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_012098820.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCGA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCCCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TAACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTGTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGTCAAT 
AAAGAGGACA TGAGCACAAA ACTGCCTCCT CTTAATAGTG ATATTGGCAG CAGCAATGCT 
AATGTTCCTG ATCTGATGGA TGAGTTTATA GCAGAACGAC TTCGAAGTGG TAATGCCTCA 
ACTATGACAC GAAGAGGAAG TAGTCCAGGT AGCCTCGAAA TTCCCAAAGA CCTCCCTGAT 
ATTCTAAATA AGCAAAACCA AATGCGTCCT ATTGATGACC CAGGTGTACC CTCAGAATGG 
ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT CTTATCAGCT CAGATAGTCA TTCGGATTCT 
TTCAGCGCTT TCCAGTATGA TGGCCGAAAA TTTGACAATT TTGGTTTTGG AGCTGATACT 
GGGAATGCAT CCTCTGCTGA CATGGATTCA GGTTCTGGCC ATTATCAGAG TGCTGAAGAG 
CAAGAGGTGG CTAGTCTAAC CACACTACAC ATTGACTCAG AAACAAGCAG TCTTAATCAA 
CAAGCTTTCT GTACTGAAGT TGCAACGGTT ACTGGTTCAG AAAGTGCCTC TCCAGTCCAT 
TCAGCTCTGG GATCCAGGTC ACAGACACCT TCTCCTTCCA CACTGAATAT AGATCACATG 
GAACAGAAGG ATCTACAGCT CGATGAGAAG CTCCACCACT CTGTTCTTCA GACACCAGAT 
GACCTAGAAA TCAGTGAATT TCCATCTGAA TGCTGTAGTG TGATGGCAGG GGGCACTCTT 
ACAGGATGGC ATGCTGATGT TGCTACTGTA ATGTGGCGAA GGATGCTGGG TATTTTGGGA 
GATGTCAACG CTATTATGGA TCCTGAAATA CATGCTCAAG TTTTTGATTA CCTCTGTGAA 
CTTTGGCAGA ATCTAGCTAA GATTAGAGAT AATCTTGGCA TTTCAACTGA TAACCTGACC 
TCCCCTTCTC CACCGGTTTT AATTCCTCCA CTGAGAATTC TTACACCTTG GCTTTTTAAG 
GCAACCATGT TAACTGATAA ATATAAACAA GGTAAATTAC ATGCCTATAA ACTTATTTGT 
AATACAATGA AAAGAAGACA AGATGTTTCT CCAAATAGAG ATTTTCTAAC ACATTTCTAC 
AACATAATGC ATTGTGGATT GCTTCACATT GATCAGGATA TTGTCAATAC AATCATCAAG 
CACTGCTCAC CTCAATTTTT CTCACTTGGT TTGCCTGGTG CAACAATGCT TATTATGGAT 
TTTATTGTAG CAGCTGGTAG AGTGGCTTCT TCAGCTTTTC TCAATGCACC AAGAGTGGAA 
GCACAAGTTC TTCTGGGATC TTTGGTTTGC TTTCCCAACT TGTATTGTGA ACTGCCTGCT 
CTCCATCCCA ACATCCCTGA TATTGCTGTG TCTCAATTTA CAGATGTTAA GGAACTTATA 
ATCAAAACTG TGTTAAGCTC TGCAAGAGAT GAGCCCTCTG GTCCTGCACG ATGTGTAGCA 
CTTTGCAGTT TAGGTATTTG GATTTGTGAA GAACTAGTCC ATGAGTCTCA TCATCCTCAA 
ATTAAGGAAG CTCTGAATGT AATTTGTGTT TCCTTAAAGT TTACTAATAA AACAGTAGCC 
CATGTAGCTT GTAACATGCT TCACATGCTG GTTCATTATG TACCTAGACT TCAGATTTAC 
CAGCCTGATT CTCCCTTGAA AATTATTCAA ATTCTAATAG CCACTATTAC CCATCTTTTG 
CCAAGTACGG AAGCGTCATC TTATGAAATG GACAAGAGGT TGGTGGTATC CTTACTTCTG 
TGTCTTCTGG ACTGGATCAT GGCCTTACCT CTTAAGACAC TGCTCCAACC AGTCCATGCT 
ACAGGAGCAG AAAATGATAA AATAGAAAAA TCTGTTCTTA ACTGCATTTA TAAGGTATTA 
CATGGGTGTG TTTATGGAGC TCAGTGTTTT AGCAATCCGA GGTATTTTCC AATAAGCCTT 
TCTGATTTGG CATCTGTAGA TTATGATCCT TTTATGCATT TGGAAAGCCT AAAAGAGCCT 
GAACCTTTGC ACTCTCCTGA TTCGGAACGA TCTTCTAAAC TCCAGCCAGT AACAGAAGTG 
AAAACTCAGA TGCAGCAAGG ATTAATCTCC ATAGCTGCCC GCACTGTTAT CACACATCTG 
GTGAATCACT TGGGCCATTA TCCAATGAGC GGTGGTCCTG CTATGTTAAC AAGTCAGGTG 
TGTGAGAATC ACGACAATCA TTACAGTGAA AGCACTGAAC TTTCCCCTGA ACTCTTCGAG 
AGTCCAAATA TTCAGTTTTT TGTGTTAAAC AATACAACCT TAGTGTCCTG TATCCAGATC 
AGAGCTGAAG AGAGTATACC TGGAGGAGGT TTATCTGCTG GTCTTGCATC AGCCAATTCA 
AATGTCAGAA TTATAGTACG TGATCTTTCT GGAAAATATT CATGGGATTC TGCCATCCTG 
TATGGACCAC CTCTTGTAAG TGGTCTGTCA GAACCTACAT CTCTCGTACT TTCATTGTCT 
CACCAAGAGA AGCCAGAAGA ACCTTCTGCA TCTAATGAAT GCTTAGAAGA TATAACAGTT 
AAAGATGGGA TTTCCCTCCA GTTTAAAAGA AGTTTTAGAG AAACTGTACC AACTTGGGAC 
ACAATCAGAG ATGAAGAAGA TGTTCTTGAT GAGCTTTTGC AGTATTTAGG TGTAACCAGT 
CCTGAATGCT TACAGAGAAC TGGAGTCTCA CTTAATATTC CTGCTCCACA GCCTGTGTGC 
ATTTCTGAAA AACAGGAGAA TGATGTTATT AATGCTATCC TTAAGCAGCA TACAGAGGAA 
AAAGAATTTG TTGAGAAACA CTTTAATGAC TTAAACATGA AAGCTGCAGA ACAAGATGAA 
CCAACACCTC AGAAACCTCA GTCAGCATTT TATTATTGCA GATTGCTTCT TAGTATATTG 
GGAATGAATT CCTGGGACAA AAGGAGGAGC TTTCATCTCC TGAAGAAAAA TGAAAAACTA 
CTTAGAGAAC TTAGGAACTT GGACTCAAGA CAGTGCCGAG AGACACACAA GATTGCAGTA 
TTTTATGTTG CTGAAGGACA AGAAGACAAA CATTCCATTC TCACCAATAC AGGAGGAAGT 
CAAGCATATG AAGATTTTGT AGCTGGTCTT GGTTGGGAGG TAAATCTTAC AAACCATTGT 
GGTTTCATGG GAGGACTACA AAAAAATAAA AGCACTGGAC TGACCACACC GTATTTTGCT 
ACCTCTACAG TTGAAGTAAT ATTTCATGTG TCAACAAGAA TGCCTTCAGA TTCTGATGAC 
TCTTTGACCA AAAAATTGAG ACATTTAGGA AATGATGAAG TGCACATTGT TTGGTCAGAG 
CATACTAGAG ACTACAGGAG AGGAATTATT CCTACAGAAT TTGGTGATGT CCTTATTGTT 
ATATACCCGA TGAAAAACCA CATGTTCAGT ATCCAGATAA TGAAAAAACC AGAGGTTCCC 
TTCTTTGGTC CACTCTTTGA TGGTGCTATT GTGAATGGAA AGGTCCTACC TATTATGGTT 
CGAGCAACAG CTATAAATGC AAGCCGTGCT CTGAAATCCC TGATTCCATT GTATCAAAAC 
TTCTATGAGG AGAGAGCACG ATATCTGCAA ACGATTGTCC AGCACCATTT AGAGCCAACG 
ACATTTGAAG ATTTTGCAGC ACAGGTTTTT TCTCCAGCTC CCTACCATCA TTTACCATCT 
GATGCCGTGG GCTCCTACCC AGAGATCCTA TCCAGTGAAA CTCCCACAGC GGCGCAGGTA 
GACGGGGCCG ACCTGGCCTC TCCAATGTCT CCTCGAACGA GCAAAAGCCG CATGTCCATG 
AAGCTGCGTC GCTCCTCTGG CTCCGCCAAT AAGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011954210.2
CDS	87..6485
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILSSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_012098820.3
Organism	Ovis aries(sheep)
Definition	Ovis aries Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012098820.3

ORF Insert Sequence:

ATGTTCTCGA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCCCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TAACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTGTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGTCAAT 
AAAGAGGACA TGAGCACAAA ACTGCCTCCT CTTAATAGTG ATATTGGCAG CAGCAATGCT 
AATGTTCCTG ATCTGATGGA TGAGTTTATA GCAGAACGAC TTCGAAGTGG TAATGCCTCA 
ACTATGACAC GAAGAGGAAG TAGTCCAGGT AGCCTCGAAA TTCCCAAAGA CCTCCCTGAT 
ATTCTAAATA AGCAAAACCA AATGCGTCCT ATTGATGACC CAGGTGTACC CTCAGAATGG 
ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT CTTATCAGCT CAGATAGTCA TTCGGATTCT 
TTCAGCGCTT TCCAGTATGA TGGCCGAAAA TTTGACAATT TTGGTTTTGG AGCTGATACT 
GGGAATGCAT CCTCTGCTGA CATGGATTCA GGTTCTGGCC ATTATCAGAG TGCTGAAGAG 
CAAGAGGTGG CTAGTCTAAC CACACTACAC ATTGACTCAG AAACAAGCAG TCTTAATCAA 
CAAGCTTTCT GTACTGAAGT TGCAACGGTT ACTGGTTCAG AAAGTGCCTC TCCAGTCCAT 
TCAGCTCTGG GATCCAGGTC ACAGACACCT TCTCCTTCCA CACTGAATAT AGATCACATG 
GAACAGAAGG ATCTACAGCT CGATGAGAAG CTCCACCACT CTGTTCTTCA GACACCAGAT 
GACCTAGAAA TCAGTGAATT TCCATCTGAA TGCTGTAGTG TGATGGCAGG GGGCACTCTT 
ACAGGATGGC ATGCTGATGT TGCTACTGTA ATGTGGCGAA GGATGCTGGG TATTTTGGGA 
GATGTCAACG CTATTATGGA TCCTGAAATA CATGCTCAAG TTTTTGATTA CCTCTGTGAA 
CTTTGGCAGA ATCTAGCTAA GATTAGAGAT AATCTTGGCA TTTCAACTGA TAACCTGACC 
TCCCCTTCTC CACCGGTTTT AATTCCTCCA CTGAGAATTC TTACACCTTG GCTTTTTAAG 
GCAACCATGT TAACTGATAA ATATAAACAA GGTAAATTAC ATGCCTATAA ACTTATTTGT 
AATACAATGA AAAGAAGACA AGATGTTTCT CCAAATAGAG ATTTTCTAAC ACATTTCTAC 
AACATAATGC ATTGTGGATT GCTTCACATT GATCAGGATA TTGTCAATAC AATCATCAAG 
CACTGCTCAC CTCAATTTTT CTCACTTGGT TTGCCTGGTG CAACAATGCT TATTATGGAT 
TTTATTGTAG CAGCTGGTAG AGTGGCTTCT TCAGCTTTTC TCAATGCACC AAGAGTGGAA 
GCACAAGTTC TTCTGGGATC TTTGGTTTGC TTTCCCAACT TGTATTGTGA ACTGCCTGCT 
CTCCATCCCA ACATCCCTGA TATTGCTGTG TCTCAATTTA CAGATGTTAA GGAACTTATA 
ATCAAAACTG TGTTAAGCTC TGCAAGAGAT GAGCCCTCTG GTCCTGCACG ATGTGTAGCA 
CTTTGCAGTT TAGGTATTTG GATTTGTGAA GAACTAGTCC ATGAGTCTCA TCATCCTCAA 
ATTAAGGAAG CTCTGAATGT AATTTGTGTT TCCTTAAAGT TTACTAATAA AACAGTAGCC 
CATGTAGCTT GTAACATGCT TCACATGCTG GTTCATTATG TACCTAGACT TCAGATTTAC 
CAGCCTGATT CTCCCTTGAA AATTATTCAA ATTCTAATAG CCACTATTAC CCATCTTTTG 
CCAAGTACGG AAGCGTCATC TTATGAAATG GACAAGAGGT TGGTGGTATC CTTACTTCTG 
TGTCTTCTGG ACTGGATCAT GGCCTTACCT CTTAAGACAC TGCTCCAACC AGTCCATGCT 
ACAGGAGCAG AAAATGATAA AATAGAAAAA TCTGTTCTTA ACTGCATTTA TAAGGTATTA 
CATGGGTGTG TTTATGGAGC TCAGTGTTTT AGCAATCCGA GGTATTTTCC AATAAGCCTT 
TCTGATTTGG CATCTGTAGA TTATGATCCT TTTATGCATT TGGAAAGCCT AAAAGAGCCT 
GAACCTTTGC ACTCTCCTGA TTCGGAACGA TCTTCTAAAC TCCAGCCAGT AACAGAAGTG 
AAAACTCAGA TGCAGCAAGG ATTAATCTCC ATAGCTGCCC GCACTGTTAT CACACATCTG 
GTGAATCACT TGGGCCATTA TCCAATGAGC GGTGGTCCTG CTATGTTAAC AAGTCAGGTG 
TGTGAGAATC ACGACAATCA TTACAGTGAA AGCACTGAAC TTTCCCCTGA ACTCTTCGAG 
AGTCCAAATA TTCAGTTTTT TGTGTTAAAC AATACAACCT TAGTGTCCTG TATCCAGATC 
AGAGCTGAAG AGAGTATACC TGGAGGAGGT TTATCTGCTG GTCTTGCATC AGCCAATTCA 
AATGTCAGAA TTATAGTACG TGATCTTTCT GGAAAATATT CATGGGATTC TGCCATCCTG 
TATGGACCAC CTCTTGTAAG TGGTCTGTCA GAACCTACAT CTCTCGTACT TTCATTGTCT 
CACCAAGAGA AGCCAGAAGA ACCTTCTGCA TCTAATGAAT GCTTAGAAGA TATAACAGTT 
AAAGATGGGA TTTCCCTCCA GTTTAAAAGA AGTTTTAGAG AAACTGTACC AACTTGGGAC 
ACAATCAGAG ATGAAGAAGA TGTTCTTGAT GAGCTTTTGC AGTATTTAGG TGTAACCAGT 
CCTGAATGCT TACAGAGAAC TGGAGTCTCA CTTAATATTC CTGCTCCACA GCCTGTGTGC 
ATTTCTGAAA AACAGGAGAA TGATGTTATT AATGCTATCC TTAAGCAGCA TACAGAGGAA 
AAAGAATTTG TTGAGAAACA CTTTAATGAC TTAAACATGA AAGCTGCAGA ACAAGATGAA 
CCAACACCTC AGAAACCTCA GTCAGCATTT TATTATTGCA GATTGCTTCT TAGTATATTG 
GGAATGAATT CCTGGGACAA AAGGAGGAGC TTTCATCTCC TGAAGAAAAA TGAAAAACTA 
CTTAGAGAAC TTAGGAACTT GGACTCAAGA CAGTGCCGAG AGACACACAA GATTGCAGTA 
TTTTATGTTG CTGAAGGACA AGAAGACAAA CATTCCATTC TCACCAATAC AGGAGGAAGT 
CAAGCATATG AAGATTTTGT AGCTGGTCTT GGTTGGGAGG TAAATCTTAC AAACCATTGT 
GGTTTCATGG GAGGACTACA AAAAAATAAA AGCACTGGAC TGACCACACC GTATTTTGCT 
ACCTCTACAG TTGAAGTAAT ATTTCATGTG TCAACAAGAA TGCCTTCAGA TTCTGATGAC 
TCTTTGACCA AAAAATTGAG ACATTTAGGA AATGATGAAG TGCACATTGT TTGGTCAGAG 
CATACTAGAG ACTACAGGAG AGGAATTATT CCTACAGAAT TTGGTGATGT CCTTATTGTT 
ATATACCCGA TGAAAAACCA CATGTTCAGT ATCCAGATAA TGAAAAAACC AGAGGTTCCC 
TTCTTTGGTC CACTCTTTGA TGGTGCTATT GTGAATGGAA AGGTCCTACC TATTATGGTT 
CGAGCAACAG CTATAAATGC AAGCCGTGCT CTGAAATCCC TGATTCCATT GTATCAAAAC 
TTCTATGAGG AGAGAGCACG ATATCTGCAA ACGATTGTCC AGCACCATTT AGAGCCAACG 
ACATTTGAAG ATTTTGCAGC ACAGGTTTTT TCTCCAGCTC CCTACCATCA TTTACCATCT 
GATGCCGTGG GCTCCTACCC AGAGATCCTA TCCAGTGAAA CTCCCACAGC GGCGCAGGTA 
GACGGGGCCG ACCTGGCCTC TCCAATGTCT CCTCGAACGA GCAAAAGCCG CATGTCCATG 
AAGCTGCGTC GCTCCTCTGG CTCCGCCAAT AAGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_012098821.3 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011954211.2 Ovis aries Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X6, mRNA.

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CloneID	OSm67925
Clone ID Related Accession (Same CDS sequence)	XM_012098821.3
Accession Version	XM_012098821.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	ral GTPase-activating protein subunit alpha-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040269.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_012098821.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCGA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCCCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TAACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAGCCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGTGGG CTCCTACCCA 
GAGATCCTAT CCAGTGAAAC TCCCACAGCG GCGCAGGTAG ACGGGGCCGA CCTGGCCTCT 
CCAATGTCTC CTCGAACGAG CAAAAGCCGC ATGTCCATGA AGCTGCGTCG CTCCTCTGGC 
TCCGCCAATA AGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011954211.2
CDS	87..6344
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILSSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_012098821.3
Organism	Ovis aries(sheep)
Definition	Ovis aries Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012098821.3

ORF Insert Sequence:

ATGTTCTCGA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCCCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TAACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAGCCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGTGGG CTCCTACCCA 
GAGATCCTAT CCAGTGAAAC TCCCACAGCG GCGCAGGTAG ACGGGGCCGA CCTGGCCTCT 
CCAATGTCTC CTCGAACGAG CAAAAGCCGC ATGTCCATGA AGCTGCGTCG CTCCTCTGGC 
TCCGCCAATA AGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_004017895.4 ) cDNA ORF clone, Ovis aries(sheep) -> XP_004017944.2 Ovis aries Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X7, mRNA.

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CloneID	OSm67926
Clone ID Related Accession (Same CDS sequence)	XM_004017895.4
Accession Version	XM_004017895.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	ral GTPase-activating protein subunit alpha-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040269.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_004017895.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCGA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCCCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TAACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAGCCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGGCTC CTACCCAGAG 
ATCCTATCCA GTGAAACTCC CACAGCGGCG CAGGTAGACG GGGCCGACCT GGCCTCTCCA 
ATGTCTCCTC GAACGAGCAA AAGCCGCATG TCCATGAAGC TGCGTCGCTC CTCTGGCTCC 
GCCAATAAGT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004017944.2
CDS	87..6341
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAGSYPEILSSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_004017895.4
Organism	Ovis aries(sheep)
Definition	Ovis aries Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004017895.4

ORF Insert Sequence:

ATGTTCTCGA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCCCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TAACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAGCCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGGCTC CTACCCAGAG 
ATCCTATCCA GTGAAACTCC CACAGCGGCG CAGGTAGACG GGGCCGACCT GGCCTCTCCA 
ATGTCTCCTC GAACGAGCAA AAGCCGCATG TCCATGAAGC TGCGTCGCTC CTCTGGCTCC 
GCCAATAAGT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_004017895.3 ) cDNA ORF clone, Ovis aries(sheep) -> XP_004017944.1 Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X15, mRNA.

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CloneID	OSm14257
Clone ID Related Accession (Same CDS sequence)	XM_004017895.3
Accession Version	XM_004017895.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	ral GTPase-activating protein subunit alpha-1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_004017895.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCC GGGGACAGGG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCCCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TAACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAGCCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGGCTC CTACCCAGAG 
ATCCTATCCA GTGAAACTCC CACAGCGGCG CAGGTAGACG GGGCCGACCT GGCCTCTCCA 
ATGTCTCCTC GAACGAGCAA AAGCCGCATG TCCATGAAGC TACGTCGCTC CTCTGGCTCC 
GCCAATAAGT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004017944.1
CDS	457..6711
Translation	MFSKKPPGTGKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAGSYPEILSSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_004017895.3
Organism	Ovis aries(sheep)
Definition	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004017895.3

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCC GGGGACAGGG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCCCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TAACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAGCCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGGCTC CTACCCAGAG 
ATCCTATCCA GTGAAACTCC CACAGCGGCG CAGGTAGACG GGGCCGACCT GGCCTCTCCA 
ATGTCTCCTC GAACGAGCAA AAGCCGCATG TCCATGAAGC TACGTCGCTC CTCTGGCTCC 
GCCAATAAGT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_012098809.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011954199.1 Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X2, mRNA.

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CloneID	OSm41096
Clone ID Related Accession (Same CDS sequence)	XM_012098809.2
Accession Version	XM_012098809.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	ral GTPase-activating protein subunit alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012098809.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCC GGGGACAGGG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCCCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAACGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTAACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GCCATGAGAA GCCGATCCAT TGGTGAATGT 
GCTCTGCCAT CGGCCTATAT ACGCAGTGCT AAAAGTGCTC CTGTTCTGAT CCATACTTCC 
AAACCCTTCT TGCCTGATAT TGTTCTCACT CCCCTTTCTG ATGAGCTTTC AGATATTGAT 
GATGCTCAAA TACTTCCTCG TCCAACTAGA GTCAGACATT TTTCGCAAAG TGAAGACACT 
GGAAATGAAG TTTTTGGTGC TTTGAGTGAG GAGCAGCCAT TGCCTCGAAG TAGCAGCACT 
TCTGACATCT TGGAACCATT CACTGTTGAA CGAGCCAAAG GTGCAGTTCC TGTCATTGAC 
AGTTCATCCC GTCATGCACC AAGCTTGCAG AGTTCCACAG AGGCTTCTTC AATAACTAGA 
TCCACTGAGA GCCACATCAC TGATACTCAT AGTAGAGAGT CTTCTCTGGA AGTTGGTGAT 
AGCATATATG ACCATCTCTG TCACTTAATA GATCCAGTAG AACTTGCAGA TTCAGCTTTT 
GAACAAATCC AGTACATTGA CCTTGAAGGA GATGATGATC TTCTTTCCTC CCTGAAAGAA 
TATTTTAAGG AAACCCAAGA AAGTCACAGT AAAAACGAGT TAGATAAAGA TGCAGCTTCG 
CAGGAAGTGA TCTCTGTTGC AGTAAATAAG GATGAAAGAT CATCCTCAGA TAAATTAGAG 
TACATAGACC AGGAAAATAA ATCAGAAAAT ACCACCTCTT TTGTTGGAAC TCCTGAAAAC 
ATACAATTTC AAAAAGAACC AAACTCTACT GTCTTCACAA ATATTAGTGC ACCCAACCAG 
TTAGACAGCT TTACGAAAAC AAAAACTTCT GAAAAATGTA AACAACTAAA CAATGATAAA 
CAGTCATCGG AACCTAGTTC AGGTAGCCCT TGTGATAAAG AAAAGAGAAA ACATCTGTAT 
AGACAAACAG CCACAGAATT AGATGCCTGT ATAGATACCA CACTAGCTGA AAAGGTGAAG 
GGTGTGCAAA TTAATGAAAA AATCACTGTC CCACATGTTT TGCATGCCAA GAAAACTACA 
CTAAAAGCAC CTGTTAACCG TAGAATGCCT CATGTTACAA GCACTAGCAA GCTTTCTCCA 
ACTAAAAGGA GCTTGTCTGA AACAGTAACT CACAGAGCCA AAATCATGAA AATTGCTACT 
AAAAAGCGAA ATAGTGTTCA TGTTACTTTT AGACCTTCTA CTGAGTCAGT TCAGTTTTAT 
AATCCTCTGG AAAACAAAGA GGCTCCATGG AAGATGAGAC TGCGGAAGCT CAGTGGCTTT 
AGTGGTAGTA ATAGCAACAT CAGCAACCCC CACACCAGCT CCAACAGTGC TGCAGAGCTA 
GTAAAACCTG GTGTGTACAG GCCTCTAGAT ACAATTAGTG CAGCATCAGC AAGCAGTGAA 
ACAATTAAGG AAACGACAGA GATTCCCACC GCCATATTAC AGAAAGAAGG AATTGCAACT 
AATCAGTTAG GTAGTCGTAG TACTATTAAG TCATCAAGTC ACGAGGCAGG ACTACCGCAG 
GGCTCCCTGG GTGGCGTTTA TAAAACTGTT GTCCATGCTC TTTCGAAGCC GAAGGCAAAT 
GTTTCCCCAC AGAGGCAGAA CAGAATGCCA CCAGAGGCTC CACTGAGGGA TCTGTACAGT 
CATGTAATGG GCTATTTTGG AAGGAAAGCT GCAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAGCCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGTGGG CTCCTACCCA 
GAGATCCTAT CCAGTGAAAC TCCCACAGCG GCGCAGGTAG ACGGGGCCGA CCTGGCCTCT 
CCAATGTCTC CTCGAACGAG CAAAAGCCGC ATGTCCATGA AGCTACGTCG CTCCTCTGGC 
TCCGCCAATA AGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011954199.1
CDS	457..8274
Translation	MFSKKPPGTGKKSTQKVLDTKKDALTRLKHLRIVIGRIFTAEPPEKPREHENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQENKSENTTSFVGTPENIQFQKEPNSTVFTNISAPNQLDSFTKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDTTLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILSSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_012098809.2
Organism	Ovis aries(sheep)
Definition	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012098809.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCC GGGGACAGGG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCCCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAACGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTAACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GCCATGAGAA GCCGATCCAT TGGTGAATGT 
GCTCTGCCAT CGGCCTATAT ACGCAGTGCT AAAAGTGCTC CTGTTCTGAT CCATACTTCC 
AAACCCTTCT TGCCTGATAT TGTTCTCACT CCCCTTTCTG ATGAGCTTTC AGATATTGAT 
GATGCTCAAA TACTTCCTCG TCCAACTAGA GTCAGACATT TTTCGCAAAG TGAAGACACT 
GGAAATGAAG TTTTTGGTGC TTTGAGTGAG GAGCAGCCAT TGCCTCGAAG TAGCAGCACT 
TCTGACATCT TGGAACCATT CACTGTTGAA CGAGCCAAAG GTGCAGTTCC TGTCATTGAC 
AGTTCATCCC GTCATGCACC AAGCTTGCAG AGTTCCACAG AGGCTTCTTC AATAACTAGA 
TCCACTGAGA GCCACATCAC TGATACTCAT AGTAGAGAGT CTTCTCTGGA AGTTGGTGAT 
AGCATATATG ACCATCTCTG TCACTTAATA GATCCAGTAG AACTTGCAGA TTCAGCTTTT 
GAACAAATCC AGTACATTGA CCTTGAAGGA GATGATGATC TTCTTTCCTC CCTGAAAGAA 
TATTTTAAGG AAACCCAAGA AAGTCACAGT AAAAACGAGT TAGATAAAGA TGCAGCTTCG 
CAGGAAGTGA TCTCTGTTGC AGTAAATAAG GATGAAAGAT CATCCTCAGA TAAATTAGAG 
TACATAGACC AGGAAAATAA ATCAGAAAAT ACCACCTCTT TTGTTGGAAC TCCTGAAAAC 
ATACAATTTC AAAAAGAACC AAACTCTACT GTCTTCACAA ATATTAGTGC ACCCAACCAG 
TTAGACAGCT TTACGAAAAC AAAAACTTCT GAAAAATGTA AACAACTAAA CAATGATAAA 
CAGTCATCGG AACCTAGTTC AGGTAGCCCT TGTGATAAAG AAAAGAGAAA ACATCTGTAT 
AGACAAACAG CCACAGAATT AGATGCCTGT ATAGATACCA CACTAGCTGA AAAGGTGAAG 
GGTGTGCAAA TTAATGAAAA AATCACTGTC CCACATGTTT TGCATGCCAA GAAAACTACA 
CTAAAAGCAC CTGTTAACCG TAGAATGCCT CATGTTACAA GCACTAGCAA GCTTTCTCCA 
ACTAAAAGGA GCTTGTCTGA AACAGTAACT CACAGAGCCA AAATCATGAA AATTGCTACT 
AAAAAGCGAA ATAGTGTTCA TGTTACTTTT AGACCTTCTA CTGAGTCAGT TCAGTTTTAT 
AATCCTCTGG AAAACAAAGA GGCTCCATGG AAGATGAGAC TGCGGAAGCT CAGTGGCTTT 
AGTGGTAGTA ATAGCAACAT CAGCAACCCC CACACCAGCT CCAACAGTGC TGCAGAGCTA 
GTAAAACCTG GTGTGTACAG GCCTCTAGAT ACAATTAGTG CAGCATCAGC AAGCAGTGAA 
ACAATTAAGG AAACGACAGA GATTCCCACC GCCATATTAC AGAAAGAAGG AATTGCAACT 
AATCAGTTAG GTAGTCGTAG TACTATTAAG TCATCAAGTC ACGAGGCAGG ACTACCGCAG 
GGCTCCCTGG GTGGCGTTTA TAAAACTGTT GTCCATGCTC TTTCGAAGCC GAAGGCAAAT 
GTTTCCCCAC AGAGGCAGAA CAGAATGCCA CCAGAGGCTC CACTGAGGGA TCTGTACAGT 
CATGTAATGG GCTATTTTGG AAGGAAAGCT GCAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAGCCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGTGGG CTCCTACCCA 
GAGATCCTAT CCAGTGAAAC TCCCACAGCG GCGCAGGTAG ACGGGGCCGA CCTGGCCTCT 
CCAATGTCTC CTCGAACGAG CAAAAGCCGC ATGTCCATGA AGCTACGTCG CTCCTCTGGC 
TCCGCCAATA AGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_012098810.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011954200.1 Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X3, mRNA.

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CloneID	OSm41097
Clone ID Related Accession (Same CDS sequence)	XM_012098810.2
Accession Version	XM_012098810.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	ral GTPase-activating protein subunit alpha-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012098810.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCC GGGGACAGGG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCCCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAACGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTAACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GCCATGAGAA GCCGATCCAT TGGTGAATGT 
GCTCTGCCAT CGGCCTATAT ACGCAGTGCT AAAAGTGCTC CTGTTCTGAT CCATACTTCC 
AAACCCTTCT TGCCTGATAT TGTTCTCACT CCCCTTTCTG ATGAGCTTTC AGATATTGAT 
GATGCTCAAA TACTTCCTCG TCCAACTAGA GTCAGACATT TTTCGCAAAG TGAAGACACT 
GGAAATGAAG TTTTTGGTGC TTTGAGTGAG GAGCAGCCAT TGCCTCGAAG TAGCAGCACT 
TCTGACATCT TGGAACCATT CACTGTTGAA CGAGCCAAAG GTGCAGTTCC TGTCATTGAC 
AGTTCATCCC GTCATGCACC AAGCTTGCAG AGTTCCACAG AGGCTTCTTC AATAACTAGA 
TCCACTGAGA GCCACATCAC TGATACTCAT AGTAGAGAGT CTTCTCTGGA AGTTGGTGAT 
AGCATATATG ACCATCTCTG TCACTTAATA GATCCAGTAG AACTTGCAGA TTCAGCTTTT 
GAACAAATCC AGTACATTGA CCTTGAAGGA GATGATGATC TTCTTTCCTC CCTGAAAGAA 
TATTTTAAGG AAACCCAAGA AAGTCACAGT AAAAACGAGT TAGATAAAGA TGCAGCTTCG 
CAGGAAGTGA TCTCTGTTGC AGTAAATAAG GATGAAAGAT CATCCTCAGA TAAATTAGAG 
TACATAGACC AGGAAAATAA ATCAGAAAAT ACCACCTCTT TTGTTGGAAC TCCTGAAAAC 
ATACAATTTC AAAAAGAACC AAACTCTACT GTCTTCACAA ATATTAGTGC ACCCAACCAG 
TTAGACAGCT TTACGAAAAC AAAAACTTCT GAAAAATGTA AACAACTAAA CAATGATAAA 
CAGTCATCGG AACCTAGTTC AGGTAGCCCT TGTGATAAAG AAAAGAGAAA ACATCTGTAT 
AGACAAACAG CCACAGAATT AGATGCCTGT ATAGATACCA CACTAGCTGA AAAGGTGAAG 
GGTGTGCAAA TTAATGAAAA AATCACTGTC CCACATGTTT TGCATGCCAA GAAAACTACA 
CTAAAAGCAC CTGTTAACCG TAGAATGCCT CATGTTACAA GCACTAGCAA GCTTTCTCCA 
ACTAAAAGGA GCTTGTCTGA AACAGTAACT CACAGAGCCA AAATCATGAA AATTGCTACT 
AAAAAGCGAA ATAGTGTTCA TGTTACTTTT AGACCTTCTA CTGAGTCAGT TCAGTTTTAT 
AATCCTCTGG AAAACAAAGA GGCTCCATGG AAGATGAGAC TGCGGAAGCT CAGTGGCTTT 
AGTGGTAGTA ATAGCAACAT CAGCAACCCC CACACCAGCT CCAACAGTGC TGCAGAGCTA 
GTAAAACCTG GTGTGTACAG GCCTCTAGAT ACAATTAGTG CAGCATCAGC AAGCAGTGAA 
ACAATTAAGG AAACGACAGA GATTCCCACC GCCATATTAC AGAAAGAAGG AATTGCAACT 
AATCAGTTAG GTAGTCGTAG TACTATTAAG TCATCAAGTC ACGAGGCAGG ACTACCGCAG 
GGCTCCCTGG GTGGCGTTTA TAAAACTGTT GTCCATGCTC TTTCGAAGCC GAAGGCAAAT 
GTTTCCCCAC AGAGGCAGAA CAGAATGCCA CCAGAGGCTC CACTGAGGGA TCTGTACAGT 
CATGTAATGG GCTATTTTGG AAGGAAAGCT GCAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAGCCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGGCTC CTACCCAGAG 
ATCCTATCCA GTGAAACTCC CACAGCGGCG CAGGTAGACG GGGCCGACCT GGCCTCTCCA 
ATGTCTCCTC GAACGAGCAA AAGCCGCATG TCCATGAAGC TACGTCGCTC CTCTGGCTCC 
GCCAATAAGT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011954200.1
CDS	457..8271
Translation	MFSKKPPGTGKKSTQKVLDTKKDALTRLKHLRIVIGRIFTAEPPEKPREHENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQENKSENTTSFVGTPENIQFQKEPNSTVFTNISAPNQLDSFTKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDTTLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAGSYPEILSSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_012098810.2
Organism	Ovis aries(sheep)
Definition	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012098810.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCC GGGGACAGGG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCCCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAACGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTAACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GCCATGAGAA GCCGATCCAT TGGTGAATGT 
GCTCTGCCAT CGGCCTATAT ACGCAGTGCT AAAAGTGCTC CTGTTCTGAT CCATACTTCC 
AAACCCTTCT TGCCTGATAT TGTTCTCACT CCCCTTTCTG ATGAGCTTTC AGATATTGAT 
GATGCTCAAA TACTTCCTCG TCCAACTAGA GTCAGACATT TTTCGCAAAG TGAAGACACT 
GGAAATGAAG TTTTTGGTGC TTTGAGTGAG GAGCAGCCAT TGCCTCGAAG TAGCAGCACT 
TCTGACATCT TGGAACCATT CACTGTTGAA CGAGCCAAAG GTGCAGTTCC TGTCATTGAC 
AGTTCATCCC GTCATGCACC AAGCTTGCAG AGTTCCACAG AGGCTTCTTC AATAACTAGA 
TCCACTGAGA GCCACATCAC TGATACTCAT AGTAGAGAGT CTTCTCTGGA AGTTGGTGAT 
AGCATATATG ACCATCTCTG TCACTTAATA GATCCAGTAG AACTTGCAGA TTCAGCTTTT 
GAACAAATCC AGTACATTGA CCTTGAAGGA GATGATGATC TTCTTTCCTC CCTGAAAGAA 
TATTTTAAGG AAACCCAAGA AAGTCACAGT AAAAACGAGT TAGATAAAGA TGCAGCTTCG 
CAGGAAGTGA TCTCTGTTGC AGTAAATAAG GATGAAAGAT CATCCTCAGA TAAATTAGAG 
TACATAGACC AGGAAAATAA ATCAGAAAAT ACCACCTCTT TTGTTGGAAC TCCTGAAAAC 
ATACAATTTC AAAAAGAACC AAACTCTACT GTCTTCACAA ATATTAGTGC ACCCAACCAG 
TTAGACAGCT TTACGAAAAC AAAAACTTCT GAAAAATGTA AACAACTAAA CAATGATAAA 
CAGTCATCGG AACCTAGTTC AGGTAGCCCT TGTGATAAAG AAAAGAGAAA ACATCTGTAT 
AGACAAACAG CCACAGAATT AGATGCCTGT ATAGATACCA CACTAGCTGA AAAGGTGAAG 
GGTGTGCAAA TTAATGAAAA AATCACTGTC CCACATGTTT TGCATGCCAA GAAAACTACA 
CTAAAAGCAC CTGTTAACCG TAGAATGCCT CATGTTACAA GCACTAGCAA GCTTTCTCCA 
ACTAAAAGGA GCTTGTCTGA AACAGTAACT CACAGAGCCA AAATCATGAA AATTGCTACT 
AAAAAGCGAA ATAGTGTTCA TGTTACTTTT AGACCTTCTA CTGAGTCAGT TCAGTTTTAT 
AATCCTCTGG AAAACAAAGA GGCTCCATGG AAGATGAGAC TGCGGAAGCT CAGTGGCTTT 
AGTGGTAGTA ATAGCAACAT CAGCAACCCC CACACCAGCT CCAACAGTGC TGCAGAGCTA 
GTAAAACCTG GTGTGTACAG GCCTCTAGAT ACAATTAGTG CAGCATCAGC AAGCAGTGAA 
ACAATTAAGG AAACGACAGA GATTCCCACC GCCATATTAC AGAAAGAAGG AATTGCAACT 
AATCAGTTAG GTAGTCGTAG TACTATTAAG TCATCAAGTC ACGAGGCAGG ACTACCGCAG 
GGCTCCCTGG GTGGCGTTTA TAAAACTGTT GTCCATGCTC TTTCGAAGCC GAAGGCAAAT 
GTTTCCCCAC AGAGGCAGAA CAGAATGCCA CCAGAGGCTC CACTGAGGGA TCTGTACAGT 
CATGTAATGG GCTATTTTGG AAGGAAAGCT GCAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAGCCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGGCTC CTACCCAGAG 
ATCCTATCCA GTGAAACTCC CACAGCGGCG CAGGTAGACG GGGCCGACCT GGCCTCTCCA 
ATGTCTCCTC GAACGAGCAA AAGCCGCATG TCCATGAAGC TACGTCGCTC CTCTGGCTCC 
GCCAATAAGT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_012098811.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011954201.1 Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X4, mRNA.

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CloneID	OSm41098
Clone ID Related Accession (Same CDS sequence)	XM_012098811.2
Accession Version	XM_012098811.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	ral GTPase-activating protein subunit alpha-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012098811.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCC GGGGACAGGG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCCCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TAACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCGCAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AATAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAACATACA ATTTCAAAAA GAACCAAACT CTACTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTACG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TACCACACTA 
GCTGAAAAGG TGAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCA 
TCAGCAAGCA GTGAAACAAT TAAGGAAACG ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC TCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAA CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTCCC TGATATTCTA 
AATAAGCAAA ACCAAATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAGT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCTGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATTGAC TCAGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG AAGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTGA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA GAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTCTG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAACATA 
ATGCATTGTG GATTGCTTCA CATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCAACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAACTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCACAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACGGAAGCGT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTT CTTAACTGCA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCTTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAGATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATCACACA TCTGGTGAAT 
CACTTGGGCC ATTATCCAAT GAGCGGTGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CTGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT GTCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATC 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GAACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTTAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACA 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCCTGGG ACAAAAGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGACTGACCA CACCGTATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA TGTGTCAACA AGAATGCCTT CAGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTTATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCCCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGATATCT GCAAACGATT GTCCAGCACC ATTTAGAGCC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GTGGGCTCCT ACCCAGAGAT CCTATCCAGT GAAACTCCCA CAGCGGCGCA GGTAGACGGG 
GCCGACCTGG CCTCTCCAAT GTCTCCTCGA ACGAGCAAAA GCCGCATGTC CATGAAGCTA 
CGTCGCTCCT CTGGCTCCGC CAATAAGTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011954201.1
CDS	457..8229
Translation	MFSKKPPGTGKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQENKSENTTSFVGTPENIQFQKEPNSTVFTNISAPNQLDSFTKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDTTLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILSSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_012098811.2
Organism	Ovis aries(sheep)
Definition	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012098811.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCC GGGGACAGGG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCCCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TAACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCGCAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AATAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAACATACA ATTTCAAAAA GAACCAAACT CTACTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTACG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TACCACACTA 
GCTGAAAAGG TGAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCA 
TCAGCAAGCA GTGAAACAAT TAAGGAAACG ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC TCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAA CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTCCC TGATATTCTA 
AATAAGCAAA ACCAAATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAGT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCTGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATTGAC TCAGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG AAGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTGA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA GAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTCTG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAACATA 
ATGCATTGTG GATTGCTTCA CATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCAACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAACTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCACAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACGGAAGCGT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTT CTTAACTGCA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCTTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAGATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATCACACA TCTGGTGAAT 
CACTTGGGCC ATTATCCAAT GAGCGGTGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CTGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT GTCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATC 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GAACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTTAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACA 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCCTGGG ACAAAAGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGACTGACCA CACCGTATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA TGTGTCAACA AGAATGCCTT CAGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTTATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCCCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGATATCT GCAAACGATT GTCCAGCACC ATTTAGAGCC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GTGGGCTCCT ACCCAGAGAT CCTATCCAGT GAAACTCCCA CAGCGGCGCA GGTAGACGGG 
GCCGACCTGG CCTCTCCAAT GTCTCCTCGA ACGAGCAAAA GCCGCATGTC CATGAAGCTA 
CGTCGCTCCT CTGGCTCCGC CAATAAGTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_012098812.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011954202.1 Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X5, mRNA.

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CloneID	OSm41099
Clone ID Related Accession (Same CDS sequence)	XM_012098812.2
Accession Version	XM_012098812.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	ral GTPase-activating protein subunit alpha-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012098812.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCC GGGGACAGGG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCCCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAACGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTAACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GATATTGATG ATGCTCAAAT ACTTCCTCGT 
CCAACTAGAG TCAGACATTT TTCGCAAAGT GAAGACACTG GAAATGAAGT TTTTGGTGCT 
TTGAGTGAGG AGCAGCCATT GCCTCGAAGT AGCAGCACTT CTGACATCTT GGAACCATTC 
ACTGTTGAAC GAGCCAAAGG TGCAGTTCCT GTCATTGACA GTTCATCCCG TCATGCACCA 
AGCTTGCAGA GTTCCACAGA GGCTTCTTCA ATAACTAGAT CCACTGAGAG CCACATCACT 
GATACTCATA GTAGAGAGTC TTCTCTGGAA GTTGGTGATA GCATATATGA CCATCTCTGT 
CACTTAATAG ATCCAGTAGA ACTTGCAGAT TCAGCTTTTG AACAAATCCA GTACATTGAC 
CTTGAAGGAG ATGATGATCT TCTTTCCTCC CTGAAAGAAT ATTTTAAGGA AACCCAAGAA 
AGTCACAGTA AAAACGAGTT AGATAAAGAT GCAGCTTCGC AGGAAGTGAT CTCTGTTGCA 
GTAAATAAGG ATGAAAGATC ATCCTCAGAT AAATTAGAGT ACATAGACCA GGAAAATAAA 
TCAGAAAATA CCACCTCTTT TGTTGGAACT CCTGAAAACA TACAATTTCA AAAAGAACCA 
AACTCTACTG TCTTCACAAA TATTAGTGCA CCCAACCAGT TAGACAGCTT TACGAAAACA 
AAAACTTCTG AAAAATGTAA ACAACTAAAC AATGATAAAC AGTCATCGGA ACCTAGTTCA 
GGTAGCCCTT GTGATAAAGA AAAGAGAAAA CATCTGTATA GACAAACAGC CACAGAATTA 
GATGCCTGTA TAGATACCAC ACTAGCTGAA AAGGTGAAGG GTGTGCAAAT TAATGAAAAA 
ATCACTGTCC CACATGTTTT GCATGCCAAG AAAACTACAC TAAAAGCACC TGTTAACCGT 
AGAATGCCTC ATGTTACAAG CACTAGCAAG CTTTCTCCAA CTAAAAGGAG CTTGTCTGAA 
ACAGTAACTC ACAGAGCCAA AATCATGAAA ATTGCTACTA AAAAGCGAAA TAGTGTTCAT 
GTTACTTTTA GACCTTCTAC TGAGTCAGTT CAGTTTTATA ATCCTCTGGA AAACAAAGAG 
GCTCCATGGA AGATGAGACT GCGGAAGCTC AGTGGCTTTA GTGGTAGTAA TAGCAACATC 
AGCAACCCCC ACACCAGCTC CAACAGTGCT GCAGAGCTAG TAAAACCTGG TGTGTACAGG 
CCTCTAGATA CAATTAGTGC AGCATCAGCA AGCAGTGAAA CAATTAAGGA AACGACAGAG 
ATTCCCACCG CCATATTACA GAAAGAAGGA ATTGCAACTA ATCAGTTAGG TAGTCGTAGT 
ACTATTAAGT CATCAAGTCA CGAGGCAGGA CTACCGCAGG GCTCCCTGGG TGGCGTTTAT 
AAAACTGTTG TCCATGCTCT TTCGAAGCCG AAGGCAAATG TTTCCCCACA GAGGCAGAAC 
AGAATGCCAC CAGAGGCTCC ACTGAGGGAT CTGTACAGTC ATGTAATGGG CTATTTTGGA 
AGGAAAGCTG CAGTCAATAA AGAGGACATG AGCACAAAAC TGCCTCCTCT TAATAGTGAT 
ATTGGCAGCA GCAATGCTAA TGTTCCTGAT CTGATGGATG AGTTTATAGC AGAACGACTT 
CGAAGTGGTA ATGCCTCAAC TATGACACGA AGAGGAAGTA GTCCAGGTAG CCTCGAAATT 
CCCAAAGACC TCCCTGATAT TCTAAATAAG CAAAACCAAA TGCGTCCTAT TGATGACCCA 
GGTGTACCCT CAGAATGGAC TTCTCCTGCC AGTGCAGGGA GCAGTGATCT TATCAGCTCA 
GATAGTCATT CGGATTCTTT CAGCGCTTTC CAGTATGATG GCCGAAAATT TGACAATTTT 
GGTTTTGGAG CTGATACTGG GAATGCATCC TCTGCTGACA TGGATTCAGG TTCTGGCCAT 
TATCAGAGTG CTGAAGAGCA AGAGGTGGCT AGTCTAACCA CACTACACAT TGACTCAGAA 
ACAAGCAGTC TTAATCAACA AGCTTTCTGT ACTGAAGTTG CAACGGTTAC TGGTTCAGAA 
AGTGCCTCTC CAGTCCATTC AGCTCTGGGA TCCAGGTCAC AGACACCTTC TCCTTCCACA 
CTGAATATAG ATCACATGGA ACAGAAGGAT CTACAGCTCG ATGAGAAGCT CCACCACTCT 
GTTCTTCAGA CACCAGATGA CCTAGAAATC AGTGAATTTC CATCTGAATG CTGTAGTGTG 
ATGGCAGGGG GCACTCTTAC AGGATGGCAT GCTGATGTTG CTACTGTAAT GTGGCGAAGG 
ATGCTGGGTA TTTTGGGAGA TGTCAACGCT ATTATGGATC CTGAAATACA TGCTCAAGTT 
TTTGATTACC TCTGTGAACT TTGGCAGAAT CTAGCTAAGA TTAGAGATAA TCTTGGCATT 
TCAACTGATA ACCTGACCTC CCCTTCTCCA CCGGTTTTAA TTCCTCCACT GAGAATTCTT 
ACACCTTGGC TTTTTAAGGC AACCATGTTA ACTGATAAAT ATAAACAAGG TAAATTACAT 
GCCTATAAAC TTATTTGTAA TACAATGAAA AGAAGACAAG ATGTTTCTCC AAATAGAGAT 
TTTCTAACAC ATTTCTACAA CATAATGCAT TGTGGATTGC TTCACATTGA TCAGGATATT 
GTCAATACAA TCATCAAGCA CTGCTCACCT CAATTTTTCT CACTTGGTTT GCCTGGTGCA 
ACAATGCTTA TTATGGATTT TATTGTAGCA GCTGGTAGAG TGGCTTCTTC AGCTTTTCTC 
AATGCACCAA GAGTGGAAGC ACAAGTTCTT CTGGGATCTT TGGTTTGCTT TCCCAACTTG 
TATTGTGAAC TGCCTGCTCT CCATCCCAAC ATCCCTGATA TTGCTGTGTC TCAATTTACA 
GATGTTAAGG AACTTATAAT CAAAACTGTG TTAAGCTCTG CAAGAGATGA GCCCTCTGGT 
CCTGCACGAT GTGTAGCACT TTGCAGTTTA GGTATTTGGA TTTGTGAAGA ACTAGTCCAT 
GAGTCTCATC ATCCTCAAAT TAAGGAAGCT CTGAATGTAA TTTGTGTTTC CTTAAAGTTT 
ACTAATAAAA CAGTAGCCCA TGTAGCTTGT AACATGCTTC ACATGCTGGT TCATTATGTA 
CCTAGACTTC AGATTTACCA GCCTGATTCT CCCTTGAAAA TTATTCAAAT TCTAATAGCC 
ACTATTACCC ATCTTTTGCC AAGTACGGAA GCGTCATCTT ATGAAATGGA CAAGAGGTTG 
GTGGTATCCT TACTTCTGTG TCTTCTGGAC TGGATCATGG CCTTACCTCT TAAGACACTG 
CTCCAACCAG TCCATGCTAC AGGAGCAGAA AATGATAAAA TAGAAAAATC TGTTCTTAAC 
TGCATTTATA AGGTATTACA TGGGTGTGTT TATGGAGCTC AGTGTTTTAG CAATCCGAGG 
TATTTTCCAA TAAGCCTTTC TGATTTGGCA TCTGTAGATT ATGATCCTTT TATGCATTTG 
GAAAGCCTAA AAGAGCCTGA ACCTTTGCAC TCTCCTGATT CGGAACGATC TTCTAAACTC 
CAGCCAGTAA CAGAAGTGAA AACTCAGATG CAGCAAGGAT TAATCTCCAT AGCTGCCCGC 
ACTGTTATCA CACATCTGGT GAATCACTTG GGCCATTATC CAATGAGCGG TGGTCCTGCT 
ATGTTAACAA GTCAGGTGTG TGAGAATCAC GACAATCATT ACAGTGAAAG CACTGAACTT 
TCCCCTGAAC TCTTCGAGAG TCCAAATATT CAGTTTTTTG TGTTAAACAA TACAACCTTA 
GTGTCCTGTA TCCAGATCAG AGCTGAAGAG AGTATACCTG GAGGAGGTTT ATCTGCTGGT 
CTTGCATCAG CCAATTCAAA TGTCAGAATT ATAGTACGTG ATCTTTCTGG AAAATATTCA 
TGGGATTCTG CCATCCTGTA TGGACCACCT CTTGTAAGTG GTCTGTCAGA ACCTACATCT 
CTCGTACTTT CATTGTCTCA CCAAGAGAAG CCAGAAGAAC CTTCTGCATC TAATGAATGC 
TTAGAAGATA TAACAGTTAA AGATGGGATT TCCCTCCAGT TTAAAAGAAG TTTTAGAGAA 
ACTGTACCAA CTTGGGACAC AATCAGAGAT GAAGAAGATG TTCTTGATGA GCTTTTGCAG 
TATTTAGGTG TAACCAGTCC TGAATGCTTA CAGAGAACTG GAGTCTCACT TAATATTCCT 
GCTCCACAGC CTGTGTGCAT TTCTGAAAAA CAGGAGAATG ATGTTATTAA TGCTATCCTT 
AAGCAGCATA CAGAGGAAAA AGAATTTGTT GAGAAACACT TTAATGACTT AAACATGAAA 
GCTGCAGAAC AAGATGAACC AACACCTCAG AAACCTCAGT CAGCATTTTA TTATTGCAGA 
TTGCTTCTTA GTATATTGGG AATGAATTCC TGGGACAAAA GGAGGAGCTT TCATCTCCTG 
AAGAAAAATG AAAAACTACT TAGAGAACTT AGGAACTTGG ACTCAAGACA GTGCCGAGAG 
ACACACAAGA TTGCAGTATT TTATGTTGCT GAAGGACAAG AAGACAAACA TTCCATTCTC 
ACCAATACAG GAGGAAGTCA AGCATATGAA GATTTTGTAG CTGGTCTTGG TTGGGAGGTA 
AATCTTACAA ACCATTGTGG TTTCATGGGA GGACTACAAA AAAATAAAAG CACTGGACTG 
ACCACACCGT ATTTTGCTAC CTCTACAGTT GAAGTAATAT TTCATGTGTC AACAAGAATG 
CCTTCAGATT CTGATGACTC TTTGACCAAA AAATTGAGAC ATTTAGGAAA TGATGAAGTG 
CACATTGTTT GGTCAGAGCA TACTAGAGAC TACAGGAGAG GAATTATTCC TACAGAATTT 
GGTGATGTCC TTATTGTTAT ATACCCGATG AAAAACCACA TGTTCAGTAT CCAGATAATG 
AAAAAACCAG AGGTTCCCTT CTTTGGTCCA CTCTTTGATG GTGCTATTGT GAATGGAAAG 
GTCCTACCTA TTATGGTTCG AGCAACAGCT ATAAATGCAA GCCGTGCTCT GAAATCCCTG 
ATTCCATTGT ATCAAAACTT CTATGAGGAG AGAGCACGAT ATCTGCAAAC GATTGTCCAG 
CACCATTTAG AGCCAACGAC ATTTGAAGAT TTTGCAGCAC AGGTTTTTTC TCCAGCTCCC 
TACCATCATT TACCATCTGA TGCCGTGGGC TCCTACCCAG AGATCCTATC CAGTGAAACT 
CCCACAGCGG CGCAGGTAGA CGGGGCCGAC CTGGCCTCTC CAATGTCTCC TCGAACGAGC 
AAAAGCCGCA TGTCCATGAA GCTACGTCGC TCCTCTGGCT CCGCCAATAA GTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011954202.1
CDS	457..8133
Translation	MFSKKPPGTGKKSTQKVLDTKKDALTRLKHLRIVIGRIFTAEPPEKPREHENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQENKSENTTSFVGTPENIQFQKEPNSTVFTNISAPNQLDSFTKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDTTLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILSSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_012098812.2
Organism	Ovis aries(sheep)
Definition	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012098812.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCC GGGGACAGGG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCCCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAACGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTAACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GATATTGATG ATGCTCAAAT ACTTCCTCGT 
CCAACTAGAG TCAGACATTT TTCGCAAAGT GAAGACACTG GAAATGAAGT TTTTGGTGCT 
TTGAGTGAGG AGCAGCCATT GCCTCGAAGT AGCAGCACTT CTGACATCTT GGAACCATTC 
ACTGTTGAAC GAGCCAAAGG TGCAGTTCCT GTCATTGACA GTTCATCCCG TCATGCACCA 
AGCTTGCAGA GTTCCACAGA GGCTTCTTCA ATAACTAGAT CCACTGAGAG CCACATCACT 
GATACTCATA GTAGAGAGTC TTCTCTGGAA GTTGGTGATA GCATATATGA CCATCTCTGT 
CACTTAATAG ATCCAGTAGA ACTTGCAGAT TCAGCTTTTG AACAAATCCA GTACATTGAC 
CTTGAAGGAG ATGATGATCT TCTTTCCTCC CTGAAAGAAT ATTTTAAGGA AACCCAAGAA 
AGTCACAGTA AAAACGAGTT AGATAAAGAT GCAGCTTCGC AGGAAGTGAT CTCTGTTGCA 
GTAAATAAGG ATGAAAGATC ATCCTCAGAT AAATTAGAGT ACATAGACCA GGAAAATAAA 
TCAGAAAATA CCACCTCTTT TGTTGGAACT CCTGAAAACA TACAATTTCA AAAAGAACCA 
AACTCTACTG TCTTCACAAA TATTAGTGCA CCCAACCAGT TAGACAGCTT TACGAAAACA 
AAAACTTCTG AAAAATGTAA ACAACTAAAC AATGATAAAC AGTCATCGGA ACCTAGTTCA 
GGTAGCCCTT GTGATAAAGA AAAGAGAAAA CATCTGTATA GACAAACAGC CACAGAATTA 
GATGCCTGTA TAGATACCAC ACTAGCTGAA AAGGTGAAGG GTGTGCAAAT TAATGAAAAA 
ATCACTGTCC CACATGTTTT GCATGCCAAG AAAACTACAC TAAAAGCACC TGTTAACCGT 
AGAATGCCTC ATGTTACAAG CACTAGCAAG CTTTCTCCAA CTAAAAGGAG CTTGTCTGAA 
ACAGTAACTC ACAGAGCCAA AATCATGAAA ATTGCTACTA AAAAGCGAAA TAGTGTTCAT 
GTTACTTTTA GACCTTCTAC TGAGTCAGTT CAGTTTTATA ATCCTCTGGA AAACAAAGAG 
GCTCCATGGA AGATGAGACT GCGGAAGCTC AGTGGCTTTA GTGGTAGTAA TAGCAACATC 
AGCAACCCCC ACACCAGCTC CAACAGTGCT GCAGAGCTAG TAAAACCTGG TGTGTACAGG 
CCTCTAGATA CAATTAGTGC AGCATCAGCA AGCAGTGAAA CAATTAAGGA AACGACAGAG 
ATTCCCACCG CCATATTACA GAAAGAAGGA ATTGCAACTA ATCAGTTAGG TAGTCGTAGT 
ACTATTAAGT CATCAAGTCA CGAGGCAGGA CTACCGCAGG GCTCCCTGGG TGGCGTTTAT 
AAAACTGTTG TCCATGCTCT TTCGAAGCCG AAGGCAAATG TTTCCCCACA GAGGCAGAAC 
AGAATGCCAC CAGAGGCTCC ACTGAGGGAT CTGTACAGTC ATGTAATGGG CTATTTTGGA 
AGGAAAGCTG CAGTCAATAA AGAGGACATG AGCACAAAAC TGCCTCCTCT TAATAGTGAT 
ATTGGCAGCA GCAATGCTAA TGTTCCTGAT CTGATGGATG AGTTTATAGC AGAACGACTT 
CGAAGTGGTA ATGCCTCAAC TATGACACGA AGAGGAAGTA GTCCAGGTAG CCTCGAAATT 
CCCAAAGACC TCCCTGATAT TCTAAATAAG CAAAACCAAA TGCGTCCTAT TGATGACCCA 
GGTGTACCCT CAGAATGGAC TTCTCCTGCC AGTGCAGGGA GCAGTGATCT TATCAGCTCA 
GATAGTCATT CGGATTCTTT CAGCGCTTTC CAGTATGATG GCCGAAAATT TGACAATTTT 
GGTTTTGGAG CTGATACTGG GAATGCATCC TCTGCTGACA TGGATTCAGG TTCTGGCCAT 
TATCAGAGTG CTGAAGAGCA AGAGGTGGCT AGTCTAACCA CACTACACAT TGACTCAGAA 
ACAAGCAGTC TTAATCAACA AGCTTTCTGT ACTGAAGTTG CAACGGTTAC TGGTTCAGAA 
AGTGCCTCTC CAGTCCATTC AGCTCTGGGA TCCAGGTCAC AGACACCTTC TCCTTCCACA 
CTGAATATAG ATCACATGGA ACAGAAGGAT CTACAGCTCG ATGAGAAGCT CCACCACTCT 
GTTCTTCAGA CACCAGATGA CCTAGAAATC AGTGAATTTC CATCTGAATG CTGTAGTGTG 
ATGGCAGGGG GCACTCTTAC AGGATGGCAT GCTGATGTTG CTACTGTAAT GTGGCGAAGG 
ATGCTGGGTA TTTTGGGAGA TGTCAACGCT ATTATGGATC CTGAAATACA TGCTCAAGTT 
TTTGATTACC TCTGTGAACT TTGGCAGAAT CTAGCTAAGA TTAGAGATAA TCTTGGCATT 
TCAACTGATA ACCTGACCTC CCCTTCTCCA CCGGTTTTAA TTCCTCCACT GAGAATTCTT 
ACACCTTGGC TTTTTAAGGC AACCATGTTA ACTGATAAAT ATAAACAAGG TAAATTACAT 
GCCTATAAAC TTATTTGTAA TACAATGAAA AGAAGACAAG ATGTTTCTCC AAATAGAGAT 
TTTCTAACAC ATTTCTACAA CATAATGCAT TGTGGATTGC TTCACATTGA TCAGGATATT 
GTCAATACAA TCATCAAGCA CTGCTCACCT CAATTTTTCT CACTTGGTTT GCCTGGTGCA 
ACAATGCTTA TTATGGATTT TATTGTAGCA GCTGGTAGAG TGGCTTCTTC AGCTTTTCTC 
AATGCACCAA GAGTGGAAGC ACAAGTTCTT CTGGGATCTT TGGTTTGCTT TCCCAACTTG 
TATTGTGAAC TGCCTGCTCT CCATCCCAAC ATCCCTGATA TTGCTGTGTC TCAATTTACA 
GATGTTAAGG AACTTATAAT CAAAACTGTG TTAAGCTCTG CAAGAGATGA GCCCTCTGGT 
CCTGCACGAT GTGTAGCACT TTGCAGTTTA GGTATTTGGA TTTGTGAAGA ACTAGTCCAT 
GAGTCTCATC ATCCTCAAAT TAAGGAAGCT CTGAATGTAA TTTGTGTTTC CTTAAAGTTT 
ACTAATAAAA CAGTAGCCCA TGTAGCTTGT AACATGCTTC ACATGCTGGT TCATTATGTA 
CCTAGACTTC AGATTTACCA GCCTGATTCT CCCTTGAAAA TTATTCAAAT TCTAATAGCC 
ACTATTACCC ATCTTTTGCC AAGTACGGAA GCGTCATCTT ATGAAATGGA CAAGAGGTTG 
GTGGTATCCT TACTTCTGTG TCTTCTGGAC TGGATCATGG CCTTACCTCT TAAGACACTG 
CTCCAACCAG TCCATGCTAC AGGAGCAGAA AATGATAAAA TAGAAAAATC TGTTCTTAAC 
TGCATTTATA AGGTATTACA TGGGTGTGTT TATGGAGCTC AGTGTTTTAG CAATCCGAGG 
TATTTTCCAA TAAGCCTTTC TGATTTGGCA TCTGTAGATT ATGATCCTTT TATGCATTTG 
GAAAGCCTAA AAGAGCCTGA ACCTTTGCAC TCTCCTGATT CGGAACGATC TTCTAAACTC 
CAGCCAGTAA CAGAAGTGAA AACTCAGATG CAGCAAGGAT TAATCTCCAT AGCTGCCCGC 
ACTGTTATCA CACATCTGGT GAATCACTTG GGCCATTATC CAATGAGCGG TGGTCCTGCT 
ATGTTAACAA GTCAGGTGTG TGAGAATCAC GACAATCATT ACAGTGAAAG CACTGAACTT 
TCCCCTGAAC TCTTCGAGAG TCCAAATATT CAGTTTTTTG TGTTAAACAA TACAACCTTA 
GTGTCCTGTA TCCAGATCAG AGCTGAAGAG AGTATACCTG GAGGAGGTTT ATCTGCTGGT 
CTTGCATCAG CCAATTCAAA TGTCAGAATT ATAGTACGTG ATCTTTCTGG AAAATATTCA 
TGGGATTCTG CCATCCTGTA TGGACCACCT CTTGTAAGTG GTCTGTCAGA ACCTACATCT 
CTCGTACTTT CATTGTCTCA CCAAGAGAAG CCAGAAGAAC CTTCTGCATC TAATGAATGC 
TTAGAAGATA TAACAGTTAA AGATGGGATT TCCCTCCAGT TTAAAAGAAG TTTTAGAGAA 
ACTGTACCAA CTTGGGACAC AATCAGAGAT GAAGAAGATG TTCTTGATGA GCTTTTGCAG 
TATTTAGGTG TAACCAGTCC TGAATGCTTA CAGAGAACTG GAGTCTCACT TAATATTCCT 
GCTCCACAGC CTGTGTGCAT TTCTGAAAAA CAGGAGAATG ATGTTATTAA TGCTATCCTT 
AAGCAGCATA CAGAGGAAAA AGAATTTGTT GAGAAACACT TTAATGACTT AAACATGAAA 
GCTGCAGAAC AAGATGAACC AACACCTCAG AAACCTCAGT CAGCATTTTA TTATTGCAGA 
TTGCTTCTTA GTATATTGGG AATGAATTCC TGGGACAAAA GGAGGAGCTT TCATCTCCTG 
AAGAAAAATG AAAAACTACT TAGAGAACTT AGGAACTTGG ACTCAAGACA GTGCCGAGAG 
ACACACAAGA TTGCAGTATT TTATGTTGCT GAAGGACAAG AAGACAAACA TTCCATTCTC 
ACCAATACAG GAGGAAGTCA AGCATATGAA GATTTTGTAG CTGGTCTTGG TTGGGAGGTA 
AATCTTACAA ACCATTGTGG TTTCATGGGA GGACTACAAA AAAATAAAAG CACTGGACTG 
ACCACACCGT ATTTTGCTAC CTCTACAGTT GAAGTAATAT TTCATGTGTC AACAAGAATG 
CCTTCAGATT CTGATGACTC TTTGACCAAA AAATTGAGAC ATTTAGGAAA TGATGAAGTG 
CACATTGTTT GGTCAGAGCA TACTAGAGAC TACAGGAGAG GAATTATTCC TACAGAATTT 
GGTGATGTCC TTATTGTTAT ATACCCGATG AAAAACCACA TGTTCAGTAT CCAGATAATG 
AAAAAACCAG AGGTTCCCTT CTTTGGTCCA CTCTTTGATG GTGCTATTGT GAATGGAAAG 
GTCCTACCTA TTATGGTTCG AGCAACAGCT ATAAATGCAA GCCGTGCTCT GAAATCCCTG 
ATTCCATTGT ATCAAAACTT CTATGAGGAG AGAGCACGAT ATCTGCAAAC GATTGTCCAG 
CACCATTTAG AGCCAACGAC ATTTGAAGAT TTTGCAGCAC AGGTTTTTTC TCCAGCTCCC 
TACCATCATT TACCATCTGA TGCCGTGGGC TCCTACCCAG AGATCCTATC CAGTGAAACT 
CCCACAGCGG CGCAGGTAGA CGGGGCCGAC CTGGCCTCTC CAATGTCTCC TCGAACGAGC 
AAAAGCCGCA TGTCCATGAA GCTACGTCGC TCCTCTGGCT CCGCCAATAA GTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_012098813.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011954203.1 Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X6, mRNA.

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CloneID	OSm41100
Clone ID Related Accession (Same CDS sequence)	XM_012098813.2 , XM_012098814.2
Accession Version	XM_012098813.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	ral GTPase-activating protein subunit alpha-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012098813.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCC GGGGACAGGG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCCCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAACGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTAACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GCCATGAGAA GCCGATCCAT TGGTGAATGT 
GCTCTGCCAT CGGCCTATAT ACGCAGTGCT AAAAGTGCTC CTGTTCTGAT CCATACTTCC 
AAACCCTTCT TGCCTGATAT TGTTCTCACT CCCCTTTCTG ATGAGCTTTC AGATATTGAT 
GATGCTCAAA TACTTCCTCG TCCAACTAGA GTCAGACATT TTTCGCAAAG TGAAGACACT 
GGAAATGAAG TTTTTGGTGC TTTGAGTGAG GAGCAGCCAT TGCCTCGAAG TAGCAGCACT 
TCTGACATCT TGGAACCATT CACTGTTGAA CGAGCCAAAG GTGCAGTTCC TGTCATTGAC 
AGTTCATCCC GTCATGCACC AAGCTTGCAG AGTTCCACAG AGGCTTCTTC AATAACTAGA 
TCCACTGAGA GCCACATCAC TGATACTCAT AGTAGAGAGT CTTCTCTGGA AGTTGGTGAT 
AGCATATATG ACCATCTCTG TCACTTAATA GATCCAGTAG AACTTGCAGA TTCAGCTTTT 
GAACAAATCC AGTACATTGA CCTTGAAGGA GATGATGATC TTCTTTCCTC CCTGAAAGAA 
TATTTTAAGG AAACCCAAGA AAGTCACAGT AAAAACGAGT TAGATAAAGA TGCAGCTTCG 
CAGGAAGTGA TCTCTGTTGC AGTAAATAAG GATGAAAGAT CATCCTCAGA TAAATTAGAG 
TACATAGACC AGGAAAATAA ATCAGAAAAT ACCACCTCTT TTGTTGGAAC TCCTGAAAAC 
ATACAATTTC AAAAAGAACC AAACTCTACT GTCTTCACAA ATATTAGTGC ACCCAACCAG 
TTAGACAGCT TTACGAAAAC AAAAACTTCT GAAAAATGTA AACAACTAAA CAATGATAAA 
CAGTCATCGG AACCTAGTTC AGGTAGCCCT TGTGATAAAG AAAAGAGAAA ACATCTGTAT 
AGACAAACAG CCACAGAATT AGATGCCTGT ATAGATACCA CACTAGCTGA AAAGGTGAAG 
GGTGTGCAAA TTAATGAAAA AATCACTGTC CCACATGTTT TGCATGCCAA GAAAACTACA 
CTAAAAGCAC CTGTTAACCG TAGAATGCCT CATGTTACAA GCACTAGCAA GCTTTCTCCA 
ACTAAAAGGA GCTTGTCTGA AACAGTAACT CACAGAGCCA AAATCATGAA AATTGCTACT 
AAAAAGCGAA ATAGTGTTCA TGTTACTTTT AGACCTTCTA CTGAGTCAGT TCAGTTTTAT 
AATCCTCTGG AAAACAAAGA GGCTCCATGG AAGATGAGAC TGCGGAAGCT CAGTGGCTTT 
AGTGGTAGTA ATAGCAACAT CAGCAACCCC CACACCAGCT CCAACAGTGC TGCAGAGCTA 
GTAAAACCTG GTGTGTACAG GCCTCTAGAT ACAATTAGTG CAGCATCAGC AAGCAGTGAA 
ACAATTAAGG AAACGACAGA GATTCCCACC GCCATATTAC AGAAAGAAGG AATTGCAACT 
AATCAGTTAG GTAGTCGTAG TACTATTAAG TCATCAAGTC ACGAGGCAGG ACTACCGCAG 
GGCTCCCTGG GTGGCGTTTA TAAAACTGTT GTCCATGCTC TTTCGAAGCC GAAGGCAAAT 
GTTTCCCCAC AGAGGCAGAA CAGAATGCCA CCAGAGGCTC CACTGAGGGA TCTGTACAGT 
CATGTAATGG GCTATTTTGG AAGGAAAGCT GCAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAGCCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGATCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011954203.1
CDS	457..8130
Translation	MFSKKPPGTGKKSTQKVLDTKKDALTRLKHLRIVIGRIFTAEPPEKPREHENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQENKSENTTSFVGTPENIQFQKEPNSTVFTNISAPNQLDSFTKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDTTLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDADH

Target ORF information:

RefSeq Version	XM_012098813.2
Organism	Ovis aries(sheep)
Definition	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012098813.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCC GGGGACAGGG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCCCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAACGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTAACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GCCATGAGAA GCCGATCCAT TGGTGAATGT 
GCTCTGCCAT CGGCCTATAT ACGCAGTGCT AAAAGTGCTC CTGTTCTGAT CCATACTTCC 
AAACCCTTCT TGCCTGATAT TGTTCTCACT CCCCTTTCTG ATGAGCTTTC AGATATTGAT 
GATGCTCAAA TACTTCCTCG TCCAACTAGA GTCAGACATT TTTCGCAAAG TGAAGACACT 
GGAAATGAAG TTTTTGGTGC TTTGAGTGAG GAGCAGCCAT TGCCTCGAAG TAGCAGCACT 
TCTGACATCT TGGAACCATT CACTGTTGAA CGAGCCAAAG GTGCAGTTCC TGTCATTGAC 
AGTTCATCCC GTCATGCACC AAGCTTGCAG AGTTCCACAG AGGCTTCTTC AATAACTAGA 
TCCACTGAGA GCCACATCAC TGATACTCAT AGTAGAGAGT CTTCTCTGGA AGTTGGTGAT 
AGCATATATG ACCATCTCTG TCACTTAATA GATCCAGTAG AACTTGCAGA TTCAGCTTTT 
GAACAAATCC AGTACATTGA CCTTGAAGGA GATGATGATC TTCTTTCCTC CCTGAAAGAA 
TATTTTAAGG AAACCCAAGA AAGTCACAGT AAAAACGAGT TAGATAAAGA TGCAGCTTCG 
CAGGAAGTGA TCTCTGTTGC AGTAAATAAG GATGAAAGAT CATCCTCAGA TAAATTAGAG 
TACATAGACC AGGAAAATAA ATCAGAAAAT ACCACCTCTT TTGTTGGAAC TCCTGAAAAC 
ATACAATTTC AAAAAGAACC AAACTCTACT GTCTTCACAA ATATTAGTGC ACCCAACCAG 
TTAGACAGCT TTACGAAAAC AAAAACTTCT GAAAAATGTA AACAACTAAA CAATGATAAA 
CAGTCATCGG AACCTAGTTC AGGTAGCCCT TGTGATAAAG AAAAGAGAAA ACATCTGTAT 
AGACAAACAG CCACAGAATT AGATGCCTGT ATAGATACCA CACTAGCTGA AAAGGTGAAG 
GGTGTGCAAA TTAATGAAAA AATCACTGTC CCACATGTTT TGCATGCCAA GAAAACTACA 
CTAAAAGCAC CTGTTAACCG TAGAATGCCT CATGTTACAA GCACTAGCAA GCTTTCTCCA 
ACTAAAAGGA GCTTGTCTGA AACAGTAACT CACAGAGCCA AAATCATGAA AATTGCTACT 
AAAAAGCGAA ATAGTGTTCA TGTTACTTTT AGACCTTCTA CTGAGTCAGT TCAGTTTTAT 
AATCCTCTGG AAAACAAAGA GGCTCCATGG AAGATGAGAC TGCGGAAGCT CAGTGGCTTT 
AGTGGTAGTA ATAGCAACAT CAGCAACCCC CACACCAGCT CCAACAGTGC TGCAGAGCTA 
GTAAAACCTG GTGTGTACAG GCCTCTAGAT ACAATTAGTG CAGCATCAGC AAGCAGTGAA 
ACAATTAAGG AAACGACAGA GATTCCCACC GCCATATTAC AGAAAGAAGG AATTGCAACT 
AATCAGTTAG GTAGTCGTAG TACTATTAAG TCATCAAGTC ACGAGGCAGG ACTACCGCAG 
GGCTCCCTGG GTGGCGTTTA TAAAACTGTT GTCCATGCTC TTTCGAAGCC GAAGGCAAAT 
GTTTCCCCAC AGAGGCAGAA CAGAATGCCA CCAGAGGCTC CACTGAGGGA TCTGTACAGT 
CATGTAATGG GCTATTTTGG AAGGAAAGCT GCAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAGCCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGATCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_012098814.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011954204.1 Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X7, mRNA.

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CloneID	OSm41100
Clone ID Related Accession (Same CDS sequence)	XM_012098813.2 , XM_012098814.2
Accession Version	XM_012098814.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	ral GTPase-activating protein subunit alpha-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012098814.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCC GGGGACAGGG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCCCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAACGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTAACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GCCATGAGAA GCCGATCCAT TGGTGAATGT 
GCTCTGCCAT CGGCCTATAT ACGCAGTGCT AAAAGTGCTC CTGTTCTGAT CCATACTTCC 
AAACCCTTCT TGCCTGATAT TGTTCTCACT CCCCTTTCTG ATGAGCTTTC AGATATTGAT 
GATGCTCAAA TACTTCCTCG TCCAACTAGA GTCAGACATT TTTCGCAAAG TGAAGACACT 
GGAAATGAAG TTTTTGGTGC TTTGAGTGAG GAGCAGCCAT TGCCTCGAAG TAGCAGCACT 
TCTGACATCT TGGAACCATT CACTGTTGAA CGAGCCAAAG GTGCAGTTCC TGTCATTGAC 
AGTTCATCCC GTCATGCACC AAGCTTGCAG AGTTCCACAG AGGCTTCTTC AATAACTAGA 
TCCACTGAGA GCCACATCAC TGATACTCAT AGTAGAGAGT CTTCTCTGGA AGTTGGTGAT 
AGCATATATG ACCATCTCTG TCACTTAATA GATCCAGTAG AACTTGCAGA TTCAGCTTTT 
GAACAAATCC AGTACATTGA CCTTGAAGGA GATGATGATC TTCTTTCCTC CCTGAAAGAA 
TATTTTAAGG AAACCCAAGA AAGTCACAGT AAAAACGAGT TAGATAAAGA TGCAGCTTCG 
CAGGAAGTGA TCTCTGTTGC AGTAAATAAG GATGAAAGAT CATCCTCAGA TAAATTAGAG 
TACATAGACC AGGAAAATAA ATCAGAAAAT ACCACCTCTT TTGTTGGAAC TCCTGAAAAC 
ATACAATTTC AAAAAGAACC AAACTCTACT GTCTTCACAA ATATTAGTGC ACCCAACCAG 
TTAGACAGCT TTACGAAAAC AAAAACTTCT GAAAAATGTA AACAACTAAA CAATGATAAA 
CAGTCATCGG AACCTAGTTC AGGTAGCCCT TGTGATAAAG AAAAGAGAAA ACATCTGTAT 
AGACAAACAG CCACAGAATT AGATGCCTGT ATAGATACCA CACTAGCTGA AAAGGTGAAG 
GGTGTGCAAA TTAATGAAAA AATCACTGTC CCACATGTTT TGCATGCCAA GAAAACTACA 
CTAAAAGCAC CTGTTAACCG TAGAATGCCT CATGTTACAA GCACTAGCAA GCTTTCTCCA 
ACTAAAAGGA GCTTGTCTGA AACAGTAACT CACAGAGCCA AAATCATGAA AATTGCTACT 
AAAAAGCGAA ATAGTGTTCA TGTTACTTTT AGACCTTCTA CTGAGTCAGT TCAGTTTTAT 
AATCCTCTGG AAAACAAAGA GGCTCCATGG AAGATGAGAC TGCGGAAGCT CAGTGGCTTT 
AGTGGTAGTA ATAGCAACAT CAGCAACCCC CACACCAGCT CCAACAGTGC TGCAGAGCTA 
GTAAAACCTG GTGTGTACAG GCCTCTAGAT ACAATTAGTG CAGCATCAGC AAGCAGTGAA 
ACAATTAAGG AAACGACAGA GATTCCCACC GCCATATTAC AGAAAGAAGG AATTGCAACT 
AATCAGTTAG GTAGTCGTAG TACTATTAAG TCATCAAGTC ACGAGGCAGG ACTACCGCAG 
GGCTCCCTGG GTGGCGTTTA TAAAACTGTT GTCCATGCTC TTTCGAAGCC GAAGGCAAAT 
GTTTCCCCAC AGAGGCAGAA CAGAATGCCA CCAGAGGCTC CACTGAGGGA TCTGTACAGT 
CATGTAATGG GCTATTTTGG AAGGAAAGCT GCAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAGCCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGATCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011954204.1
CDS	457..8130
Translation	MFSKKPPGTGKKSTQKVLDTKKDALTRLKHLRIVIGRIFTAEPPEKPREHENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQENKSENTTSFVGTPENIQFQKEPNSTVFTNISAPNQLDSFTKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDTTLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDADH

Target ORF information:

RefSeq Version	XM_012098814.2
Organism	Ovis aries(sheep)
Definition	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012098814.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCC GGGGACAGGG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCCCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAACGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTAACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GCCATGAGAA GCCGATCCAT TGGTGAATGT 
GCTCTGCCAT CGGCCTATAT ACGCAGTGCT AAAAGTGCTC CTGTTCTGAT CCATACTTCC 
AAACCCTTCT TGCCTGATAT TGTTCTCACT CCCCTTTCTG ATGAGCTTTC AGATATTGAT 
GATGCTCAAA TACTTCCTCG TCCAACTAGA GTCAGACATT TTTCGCAAAG TGAAGACACT 
GGAAATGAAG TTTTTGGTGC TTTGAGTGAG GAGCAGCCAT TGCCTCGAAG TAGCAGCACT 
TCTGACATCT TGGAACCATT CACTGTTGAA CGAGCCAAAG GTGCAGTTCC TGTCATTGAC 
AGTTCATCCC GTCATGCACC AAGCTTGCAG AGTTCCACAG AGGCTTCTTC AATAACTAGA 
TCCACTGAGA GCCACATCAC TGATACTCAT AGTAGAGAGT CTTCTCTGGA AGTTGGTGAT 
AGCATATATG ACCATCTCTG TCACTTAATA GATCCAGTAG AACTTGCAGA TTCAGCTTTT 
GAACAAATCC AGTACATTGA CCTTGAAGGA GATGATGATC TTCTTTCCTC CCTGAAAGAA 
TATTTTAAGG AAACCCAAGA AAGTCACAGT AAAAACGAGT TAGATAAAGA TGCAGCTTCG 
CAGGAAGTGA TCTCTGTTGC AGTAAATAAG GATGAAAGAT CATCCTCAGA TAAATTAGAG 
TACATAGACC AGGAAAATAA ATCAGAAAAT ACCACCTCTT TTGTTGGAAC TCCTGAAAAC 
ATACAATTTC AAAAAGAACC AAACTCTACT GTCTTCACAA ATATTAGTGC ACCCAACCAG 
TTAGACAGCT TTACGAAAAC AAAAACTTCT GAAAAATGTA AACAACTAAA CAATGATAAA 
CAGTCATCGG AACCTAGTTC AGGTAGCCCT TGTGATAAAG AAAAGAGAAA ACATCTGTAT 
AGACAAACAG CCACAGAATT AGATGCCTGT ATAGATACCA CACTAGCTGA AAAGGTGAAG 
GGTGTGCAAA TTAATGAAAA AATCACTGTC CCACATGTTT TGCATGCCAA GAAAACTACA 
CTAAAAGCAC CTGTTAACCG TAGAATGCCT CATGTTACAA GCACTAGCAA GCTTTCTCCA 
ACTAAAAGGA GCTTGTCTGA AACAGTAACT CACAGAGCCA AAATCATGAA AATTGCTACT 
AAAAAGCGAA ATAGTGTTCA TGTTACTTTT AGACCTTCTA CTGAGTCAGT TCAGTTTTAT 
AATCCTCTGG AAAACAAAGA GGCTCCATGG AAGATGAGAC TGCGGAAGCT CAGTGGCTTT 
AGTGGTAGTA ATAGCAACAT CAGCAACCCC CACACCAGCT CCAACAGTGC TGCAGAGCTA 
GTAAAACCTG GTGTGTACAG GCCTCTAGAT ACAATTAGTG CAGCATCAGC AAGCAGTGAA 
ACAATTAAGG AAACGACAGA GATTCCCACC GCCATATTAC AGAAAGAAGG AATTGCAACT 
AATCAGTTAG GTAGTCGTAG TACTATTAAG TCATCAAGTC ACGAGGCAGG ACTACCGCAG 
GGCTCCCTGG GTGGCGTTTA TAAAACTGTT GTCCATGCTC TTTCGAAGCC GAAGGCAAAT 
GTTTCCCCAC AGAGGCAGAA CAGAATGCCA CCAGAGGCTC CACTGAGGGA TCTGTACAGT 
CATGTAATGG GCTATTTTGG AAGGAAAGCT GCAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAGCCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGATCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_012098815.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011954205.1 Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X8, mRNA.

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CloneID	OSm41101
Clone ID Related Accession (Same CDS sequence)	XM_012098815.2
Accession Version	XM_012098815.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	ral GTPase-activating protein subunit alpha-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012098815.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCC GGGGACAGGG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCCCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAACGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTAACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GCCATGAGAA GCCGATCCAT TGGTGAATGT 
GCTCTGCCAT CGGCCTATAT ACGCAGTGCT AAAAGTGCTC CTGTTCTGAT CCATACTTCC 
AAACCCTTCT TGCCTGATAT TGTTCTCACT CCCCTTTCTG ATGAGCTTTC AGATATTGAT 
GATGCTCAAA TACTTCCTCG TCCAACTAGA GTCAGACATT TTTCGCAAAG TGAAGACACT 
GGAAATGAAG TTTTTGGTGC TTTGAGTGAG GAGCAGCCAT TGCCTCGAAG TAGCAGCACT 
TCTGACATCT TGGAACCATT CACTGTTGAA CGAGCCAAAG GTGCAGTTCC TGTCATTGAC 
AGTTCATCCC GTCATGCACC AAGCTTGCAG AGTTCCACAG AGGCTTCTTC AATAACTAGA 
TCCACTGAGA GCCACATCAC TGATACTCAT AGTAGAGAGT CTTCTCTGGA AGTTGGTGAT 
AGCATATATG ACCATCTCTG TCACTTAATA GATCCAGTAG AACTTGCAGA TTCAGCTTTT 
GAACAAATCC AGTACATTGA CCTTGAAGGA GATGATGATC TTCTTTCCTC CCTGAAAGAA 
TATTTTAAGG AAACCCAAGA AAGTCACAGT AAAAACGAGT TAGATAAAGA TGCAGCTTCG 
CAGGAAGTGA TCTCTGTTGC AGTAAATAAG GATGAAAGAT CATCCTCAGA TAAATTAGAG 
TACATAGACC AGGAAAATAA ATCAGAAAAT ACCACCTCTT TTGTTGGAAC TCCTGAAAAC 
ATACAATTTC AAAAAGAACC AAACTCTACT GTCTTCACAA ATATTAGTGC ACCCAACCAG 
TTAGACAGCT TTACGAAAAC AAAAACTTCT GAAAAATGTA AACAACTAAA CAATGATAAA 
CAGTCATCGG AACCTAGTTC AGGTAGCCCT TGTGATAAAG AAAAGAGAAA ACATCTGTAT 
AGACAAACAG CCACAGAATT AGATGCCTGT ATAGATACCA CACTAGCTGA AAAGGTGAAG 
GGTGTGCAAA TTAATGAAAA AATCACTGTC CCACATGTTT TGCATGCCAA GAAAACTACA 
CTAAAAGCAC CTGTTAACCG TAGAATGCCT CATGTTACAA GCACTAGCAA GCTTTCTCCA 
ACTAAAAGGA GCTTGTCTGA AACAGTAACT CACAGAGCCA AAATCATGAA AATTGCTACT 
AAAAAGCGAA ATAGTGTTCA TGTTACTTTT AGACCTTCTA CTGAGTCAGT TCAGTTTTAT 
AATCCTCTGG AAAACAAAGA GGCTCCATGG AAGATGAGAC TGCGGAAGCT CAGTGGCTTT 
AGTGGTAGTA ATAGCAACAT CAGCAACCCC CACACCAGCT CCAACAGTGC TGCAGAGCTA 
GTAAAACCTG GTGTGTACAG GCCTCTAGAT ACAATTAGTG CAGCATCAGC AAGCAGTGAA 
ACAATTAAGG AAACGACAGA GATTCCCACC GCCATATTAC AGAAAGAAGG AATTGCAACT 
AATCAGTTAG GTAGTCGTAG TACTATTAAG TCATCAAGTC ACGAGGCAGG ACTACCGCAG 
GGCTCCCTGG GTGGCGTTTA TAAAACTGTT GTCCATGCTC TTTCGAAGCC GAAGGCAAAT 
GTTTCCCCAC AGAGGCAGAA CAGAATGCCA CCAGAGGCTC CACTGAGGGA TCTGTACAGT 
CATGTAATGG GCTATTTTGG AAGGAAAGCT GCAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TGATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011954205.1
CDS	457..8004
Translation	MFSKKPPGTGKKSTQKVLDTKKDALTRLKHLRIVIGRIFTAEPPEKPREHENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQENKSENTTSFVGTPENIQFQKEPNSTVFTNISAPNQLDSFTKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDTTLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNLI

Target ORF information:

RefSeq Version	XM_012098815.2
Organism	Ovis aries(sheep)
Definition	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012098815.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCC GGGGACAGGG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCCCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAACGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTAACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GCCATGAGAA GCCGATCCAT TGGTGAATGT 
GCTCTGCCAT CGGCCTATAT ACGCAGTGCT AAAAGTGCTC CTGTTCTGAT CCATACTTCC 
AAACCCTTCT TGCCTGATAT TGTTCTCACT CCCCTTTCTG ATGAGCTTTC AGATATTGAT 
GATGCTCAAA TACTTCCTCG TCCAACTAGA GTCAGACATT TTTCGCAAAG TGAAGACACT 
GGAAATGAAG TTTTTGGTGC TTTGAGTGAG GAGCAGCCAT TGCCTCGAAG TAGCAGCACT 
TCTGACATCT TGGAACCATT CACTGTTGAA CGAGCCAAAG GTGCAGTTCC TGTCATTGAC 
AGTTCATCCC GTCATGCACC AAGCTTGCAG AGTTCCACAG AGGCTTCTTC AATAACTAGA 
TCCACTGAGA GCCACATCAC TGATACTCAT AGTAGAGAGT CTTCTCTGGA AGTTGGTGAT 
AGCATATATG ACCATCTCTG TCACTTAATA GATCCAGTAG AACTTGCAGA TTCAGCTTTT 
GAACAAATCC AGTACATTGA CCTTGAAGGA GATGATGATC TTCTTTCCTC CCTGAAAGAA 
TATTTTAAGG AAACCCAAGA AAGTCACAGT AAAAACGAGT TAGATAAAGA TGCAGCTTCG 
CAGGAAGTGA TCTCTGTTGC AGTAAATAAG GATGAAAGAT CATCCTCAGA TAAATTAGAG 
TACATAGACC AGGAAAATAA ATCAGAAAAT ACCACCTCTT TTGTTGGAAC TCCTGAAAAC 
ATACAATTTC AAAAAGAACC AAACTCTACT GTCTTCACAA ATATTAGTGC ACCCAACCAG 
TTAGACAGCT TTACGAAAAC AAAAACTTCT GAAAAATGTA AACAACTAAA CAATGATAAA 
CAGTCATCGG AACCTAGTTC AGGTAGCCCT TGTGATAAAG AAAAGAGAAA ACATCTGTAT 
AGACAAACAG CCACAGAATT AGATGCCTGT ATAGATACCA CACTAGCTGA AAAGGTGAAG 
GGTGTGCAAA TTAATGAAAA AATCACTGTC CCACATGTTT TGCATGCCAA GAAAACTACA 
CTAAAAGCAC CTGTTAACCG TAGAATGCCT CATGTTACAA GCACTAGCAA GCTTTCTCCA 
ACTAAAAGGA GCTTGTCTGA AACAGTAACT CACAGAGCCA AAATCATGAA AATTGCTACT 
AAAAAGCGAA ATAGTGTTCA TGTTACTTTT AGACCTTCTA CTGAGTCAGT TCAGTTTTAT 
AATCCTCTGG AAAACAAAGA GGCTCCATGG AAGATGAGAC TGCGGAAGCT CAGTGGCTTT 
AGTGGTAGTA ATAGCAACAT CAGCAACCCC CACACCAGCT CCAACAGTGC TGCAGAGCTA 
GTAAAACCTG GTGTGTACAG GCCTCTAGAT ACAATTAGTG CAGCATCAGC AAGCAGTGAA 
ACAATTAAGG AAACGACAGA GATTCCCACC GCCATATTAC AGAAAGAAGG AATTGCAACT 
AATCAGTTAG GTAGTCGTAG TACTATTAAG TCATCAAGTC ACGAGGCAGG ACTACCGCAG 
GGCTCCCTGG GTGGCGTTTA TAAAACTGTT GTCCATGCTC TTTCGAAGCC GAAGGCAAAT 
GTTTCCCCAC AGAGGCAGAA CAGAATGCCA CCAGAGGCTC CACTGAGGGA TCTGTACAGT 
CATGTAATGG GCTATTTTGG AAGGAAAGCT GCAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TGATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015102008.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014957494.1 Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X9, mRNA.

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CloneID	OSm41102
Clone ID Related Accession (Same CDS sequence)	XM_015102008.1
Accession Version	XM_015102008.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	ral GTPase-activating protein subunit alpha-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTCAT GCTTAATCCC TGGATTTTCA GCACCACAGT CTGAATATGG ACCTCGAACT 
TTAGATAACC TCATTAATCC TCCACTCAAC CTACAAGAAA CTCAAGTCAC TATAGAGGAA 
ATCACTCCAC TTGTCCCCCC ACAATCAGGA GATAAAGGAC AAGAAGATCT TACAAGCTAT 
TTTCTTGAAG CACTTTTAAA ATATATAGTA ATTCAGGTAA AAAGTTTAGA GTGGAAGAAC 
AAAGAAAACC AAGAAAAAGG ATTTTCATTT CTGTTTTCAC ATTTTAAGAA ATATTATTTG 
CCTTATATTT TCCCAAACAT CTGTAAGGAG AATAGTTTAT ATCATCCTGT ACTTGATATC 
CCACAGATGA GACCAAAGCC CCATTATGTC ATGATAAAGA AGGATGCTGA AACCAATGAG 
GCAATCTATT GTACAAAGGA GCCTTTCATT AAGGCTCGTG TTATTGTCAT TCGTTGGCTG 
GTTTCATTCT GGCTGGAGCC AAAGCCGCAT ACAGGACCTC ATATTCCTGG GATGGAAGGT 
GAAGTCTTGC CAAAAAATAT TCAGAGAGCA GCTGCTAGTT TGGTATCCAG AGAAGAAAAC 
AAAAATGATA ATGCTGATAA AACAGATAGA AGTACAGAAC CTGAACAGTC TCATTCCAAC 
ACAAGCACTC TCACGGAGCG AGAACCTAGC TCTTCTAGTC TCTGTAGTAT TGATGAAGAG 
CATCTCACAG ACATTGAAAT AGTTCGCCGA GTTTTTTCTT CTAAAAGAAG TAACGTAAAC 
TTTGTCACAG AGATATTTCG TCAGGCATTT TTATTACCAA TTTGCGAAGC AGCAGCTATG 
AGAAAAGTGG TAAAAGTATA TCAAGAATGG ATCCAGCAAG AGGAAAAACC TTTGTTCATG 
CAAGAGCCTG AAGAAATTGT GATCTCTTCT TCAGACCTTC CGTGCATTGA CAATGTCACA 
GACCATGATA TTTCAATGGA AGAAGGAGAA AAAAGAGAAG AGGAAAATGG GACCAATGTT 
GCTGATCATG TTCGAAATTC CACTTGGCCT AAAAATGGAA CCTACCAAGA TATTCTTCAT 
AATGCTTCTG AAGAAGCCAT AGAACAAAAC ATACGAGCTG GTGCCCAGGC AGTTTTGCAG 
GTGTTTATTA TAAATTCTTC AAACATATTT CTTCTTGAAC CTGCAAATGA GATAAAAAGT 
CTTCTGGATG AGCACACAGA TATGTGTAAA CGAATTCTTA ACATTTATCG GTTCATGGTT 
GTGCAAGTAT CAATGGACAA AAAGACTTGG GAACAGATGC TGCTTGTGTT GCTCAGAGTC 
ACGGAGTCTG TACTGAAGAT GCCATCACAA GCTTTTCTAC AGTTCCAGGG GAAAAAAAAC 
ATGACTTTGG CAGGTCGACT TGCAGGACCA CTTTTCCAGA CTCTTATAGT TGCCTGGATT 
AAAGCAAATC TAAATGTGTA CATCTCTCGA GAACTTTGGG ATGACTTACT CTCAGTATTG 
TCATCGTTGA CCTATTGGGA AGAGTTAGCC ACTGAATGGT CGCTAACTAT GGAGACACTA 
ACTAAAGTTT TAGCTAGAAA TTTGTATAGT TTGGATCTCA GTGATTTACC ATTGGATAAG 
CTGAGTGAAC AGAAACAAAA AAAGCACAAA GGGAAAGGAG TGGGACATGA ATTTCAGAAA 
GTTTCAGTTG ACAAGTCATT TTCTAGAGGA TGGAGTCGCG ATCAGCCTGG CCAAGCCCCA 
ATGAGGCAGA GAAGTGCAAC AACCACTGGT TCCCCAGGCA CAGAAAAGGC AAGGAGTATA 
GTACGGCAAA AAACTGTTGC CATGAGAAGC CGATCCATTG GTGAATGTGC TCTGCCATCG 
GCCTATATAC GCAGTGCTAA AAGTGCTCCT GTTCTGATCC ATACTTCCAA ACCCTTCTTG 
CCTGATATTG TTCTCACTCC CCTTTCTGAT GAGCTTTCAG ATATTGATGA TGCTCAAATA 
CTTCCTCGTC CAACTAGAGT CAGACATTTT TCGCAAAGTG AAGACACTGG AAATGAAGTT 
TTTGGTGCTT TGAGTGAGGA GCAGCCATTG CCTCGAAGTA GCAGCACTTC TGACATCTTG 
GAACCATTCA CTGTTGAACG AGCCAAAGGT GCAGTTCCTG TCATTGACAG TTCATCCCGT 
CATGCACCAA GCTTGCAGAG TTCCACAGAG GCTTCTTCAA TAACTAGATC CACTGAGAGC 
CACATCACTG ATACTCATAG TAGAGAGTCT TCTCTGGAAG TTGGTGATAG CATATATGAC 
CATCTCTGTC ACTTAATAGA TCCAGTAGAA CTTGCAGATT CAGCTTTTGA ACAAATCCAG 
TACATTGACC TTGAAGGAGA TGATGATCTT CTTTCCTCCC TGAAAGAATA TTTTAAGGAA 
ACCCAAGAAA GTCACAGTAA AAACGAGTTA GATAAAGATG CAGCTTCGCA GGAAGTGATC 
TCTGTTGCAG TAAATAAGGA TGAAAGATCA TCCTCAGATA AATTAGAGTA CATAGACCAG 
GAAAATAAAT CAGAAAATAC CACCTCTTTT GTTGGAACTC CTGAAAACAT ACAATTTCAA 
AAAGAACCAA ACTCTACTGT CTTCACAAAT ATTAGTGCAC CCAACCAGTT AGACAGCTTT 
ACGAAAACAA AAACTTCTGA AAAATGTAAA CAACTAAACA ATGATAAACA GTCATCGGAA 
CCTAGTTCAG GTAGCCCTTG TGATAAAGAA AAGAGAAAAC ATCTGTATAG ACAAACAGCC 
ACAGAATTAG ATGCCTGTAT AGATACCACA CTAGCTGAAA AGGTGAAGGG TGTGCAAATT 
AATGAAAAAA TCACTGTCCC ACATGTTTTG CATGCCAAGA AAACTACACT AAAAGCACCT 
GTTAACCGTA GAATGCCTCA TGTTACAAGC ACTAGCAAGC TTTCTCCAAC TAAAAGGAGC 
TTGTCTGAAA CAGTAACTCA CAGAGCCAAA ATCATGAAAA TTGCTACTAA AAAGCGAAAT 
AGTGTTCATG TTACTTTTAG ACCTTCTACT GAGTCAGTTC AGTTTTATAA TCCTCTGGAA 
AACAAAGAGG CTCCATGGAA GATGAGACTG CGGAAGCTCA GTGGCTTTAG TGGTAGTAAT 
AGCAACATCA GCAACCCCCA CACCAGCTCC AACAGTGCTG CAGAGCTAGT AAAACCTGGT 
GTGTACAGGC CTCTAGATAC AATTAGTGCA GCATCAGCAA GCAGTGAAAC AATTAAGGAA 
ACGACAGAGA TTCCCACCGC CATATTACAG AAAGAAGGAA TTGCAACTAA TCAGTTAGGT 
AGTCGTAGTA CTATTAAGTC ATCAAGTCAC GAGGCAGGAC TACCGCAGGG CTCCCTGGGT 
GGCGTTTATA AAACTGTTGT CCATGCTCTT TCGAAGCCGA AGGCAAATGT TTCCCCACAG 
AGGCAGAACA GAATGCCACC AGAGGCTCCA CTGAGGGATC TGTACAGTCA TGTAATGGGC 
TATTTTGGAA GGAAAGCTGC AGTCAATAAA GAGGACATGA GCACAAAACT GCCTCCTCTT 
AATAGTGATA TTGGCAGCAG CAATGCTAAT GTTCCTGATC TGATGGATGA GTTTATAGCA 
GAACGACTTC GAAGTGGTAA TGCCTCAACT ATGACACGAA GAGGAAGTAG TCCAGGTAGC 
CTCGAAATTC CCAAAGACCT CCCTGATATT CTAAATAAGC AAAACCAAAT GCGTCCTATT 
GATGACCCAG GTGTACCCTC AGAATGGACT TCTCCTGCCA GTGCAGGGAG CAGTGATCTT 
ATCAGCTCAG ATAGTCATTC GGATTCTTTC AGCGCTTTCC AGTATGATGG CCGAAAATTT 
GACAATTTTG GTTTTGGAGC TGATACTGGG AATGCATCCT CTGCTGACAT GGATTCAGGT 
TCTGGCCATT ATCAGAGTGC TGAAGAGCAA GAGGTGGCTA GTCTAACCAC ACTACACATT 
GACTCAGAAA CAAGCAGTCT TAATCAACAA GCTTTCTGTA CTGAAGTTGC AACGGTTACT 
GGTTCAGAAA GTGCCTCTCC AGTCCATTCA GCTCTGGGAT CCAGGTCACA GACACCTTCT 
CCTTCCACAC TGAATATAGA TCACATGGAA CAGAAGGATC TACAGCTCGA TGAGAAGCTC 
CACCACTCTG TTCTTCAGAC ACCAGATGAC CTAGAAATCA GTGAATTTCC ATCTGAATGC 
TGTAGTGTGA TGGCAGGGGG CACTCTTACA GGATGGCATG CTGATGTTGC TACTGTAATG 
TGGCGAAGGA TGCTGGGTAT TTTGGGAGAT GTCAACGCTA TTATGGATCC TGAAATACAT 
GCTCAAGTTT TTGATTACCT CTGTGAACTT TGGCAGAATC TAGCTAAGAT TAGAGATAAT 
CTTGGCATTT CAACTGATAA CCTGACCTCC CCTTCTCCAC CGGTTTTAAT TCCTCCACTG 
AGAATTCTTA CACCTTGGCT TTTTAAGGCA ACCATGTTAA CTGATAAATA TAAACAAGGT 
AAATTACATG CCTATAAACT TATTTGTAAT ACAATGAAAA GAAGACAAGA TGTTTCTCCA 
AATAGAGATT TTCTAACACA TTTCTACAAC ATAATGCATT GTGGATTGCT TCACATTGAT 
CAGGATATTG TCAATACAAT CATCAAGCAC TGCTCACCTC AATTTTTCTC ACTTGGTTTG 
CCTGGTGCAA CAATGCTTAT TATGGATTTT ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA 
GCTTTTCTCA ATGCACCAAG AGTGGAAGCA CAAGTTCTTC TGGGATCTTT GGTTTGCTTT 
CCCAACTTGT ATTGTGAACT GCCTGCTCTC CATCCCAACA TCCCTGATAT TGCTGTGTCT 
CAATTTACAG ATGTTAAGGA ACTTATAATC AAAACTGTGT TAAGCTCTGC AAGAGATGAG 
CCCTCTGGTC CTGCACGATG TGTAGCACTT TGCAGTTTAG GTATTTGGAT TTGTGAAGAA 
CTAGTCCATG AGTCTCATCA TCCTCAAATT AAGGAAGCTC TGAATGTAAT TTGTGTTTCC 
TTAAAGTTTA CTAATAAAAC AGTAGCCCAT GTAGCTTGTA ACATGCTTCA CATGCTGGTT 
CATTATGTAC CTAGACTTCA GATTTACCAG CCTGATTCTC CCTTGAAAAT TATTCAAATT 
CTAATAGCCA CTATTACCCA TCTTTTGCCA AGTACGGAAG CGTCATCTTA TGAAATGGAC 
AAGAGGTTGG TGGTATCCTT ACTTCTGTGT CTTCTGGACT GGATCATGGC CTTACCTCTT 
AAGACACTGC TCCAACCAGT CCATGCTACA GGAGCAGAAA ATGATAAAAT AGAAAAATCT 
GTTCTTAACT GCATTTATAA GGTATTACAT GGGTGTGTTT ATGGAGCTCA GTGTTTTAGC 
AATCCGAGGT ATTTTCCAAT AAGCCTTTCT GATTTGGCAT CTGTAGATTA TGATCCTTTT 
ATGCATTTGG AAAGCCTAAA AGAGCCTGAA CCTTTGCACT CTCCTGATTC GGAACGATCT 
TCTAAACTCC AGCCAGTAAC AGAAGTGAAA ACTCAGATGC AGCAAGGATT AATCTCCATA 
GCTGCCCGCA CTGTTATCAC ACATCTGGTG AATCACTTGG GCCATTATCC AATGAGCGGT 
GGTCCTGCTA TGTTAACAAG TCAGGTGTGT GAGAATCACG ACAATCATTA CAGTGAAAGC 
ACTGAACTTT CCCCTGAACT CTTCGAGAGT CCAAATATTC AGTTTTTTGT GTTAAACAAT 
ACAACCTTAG TGTCCTGTAT CCAGATCAGA GCTGAAGAGA GTATACCTGG AGGAGGTTTA 
TCTGCTGGTC TTGCATCAGC CAATTCAAAT GTCAGAATTA TAGTACGTGA TCTTTCTGGA 
AAATATTCAT GGGATTCTGC CATCCTGTAT GGACCACCTC TTGTAAGTGG TCTGTCAGAA 
CCTACATCTC TCGTACTTTC ATTGTCTCAC CAAGAGAAGC CAGAAGAACC TTCTGCATCT 
AATGAATGCT TAGAAGATAT AACAGTTAAA GATGGGATTT CCCTCCAGTT TAAAAGAAGT 
TTTAGAGAAA CTGTACCAAC TTGGGACACA ATCAGAGATG AAGAAGATGT TCTTGATGAG 
CTTTTGCAGT ATTTAGGTGT AACCAGTCCT GAATGCTTAC AGAGAACTGG AGTCTCACTT 
AATATTCCTG CTCCACAGCC TGTGTGCATT TCTGAAAAAC AGGAGAATGA TGTTATTAAT 
GCTATCCTTA AGCAGCATAC AGAGGAAAAA GAATTTGTTG AGAAACACTT TAATGACTTA 
AACATGAAAG CTGCAGAACA AGATGAACCA ACACCTCAGA AACCTCAGTC AGCATTTTAT 
TATTGCAGAT TGCTTCTTAG TATATTGGGA ATGAATTCCT GGGACAAAAG GAGGAGCTTT 
CATCTCCTGA AGAAAAATGA AAAACTACTT AGAGAACTTA GGAACTTGGA CTCAAGACAG 
TGCCGAGAGA CACACAAGAT TGCAGTATTT TATGTTGCTG AAGGACAAGA AGACAAACAT 
TCCATTCTCA CCAATACAGG AGGAAGTCAA GCATATGAAG ATTTTGTAGC TGGTCTTGGT 
TGGGAGGTAA ATCTTACAAA CCATTGTGGT TTCATGGGAG GACTACAAAA AAATAAAAGC 
ACTGGACTGA CCACACCGTA TTTTGCTACC TCTACAGTTG AAGTAATATT TCATGTGTCA 
ACAAGAATGC CTTCAGATTC TGATGACTCT TTGACCAAAA AATTGAGACA TTTAGGAAAT 
GATGAAGTGC ACATTGTTTG GTCAGAGCAT ACTAGAGACT ACAGGAGAGG AATTATTCCT 
ACAGAATTTG GTGATGTCCT TATTGTTATA TACCCGATGA AAAACCACAT GTTCAGTATC 
CAGATAATGA AAAAACCAGA GGTTCCCTTC TTTGGTCCAC TCTTTGATGG TGCTATTGTG 
AATGGAAAGG TCCTACCTAT TATGGTTCGA GCAACAGCTA TAAATGCAAG CCGTGCTCTG 
AAATCCCTGA TTCCATTGTA TCAAAACTTC TATGAGGAGA GAGCACGATA TCTGCAAACG 
ATTGTCCAGC ACCATTTAGA GCCAACGACA TTTGAAGATT TTGCAGCACA GGTTTTTTCT 
CCAGCTCCCT ACCATCATTT ACCATCTGAT GCCGTGGGCT CCTACCCAGA GATCCTATCC 
AGTGAAACTC CCACAGCGGC GCAGGTAGAC GGGGCCGACC TGGCCTCTCC AATGTCTCCT 
CGAACGAGCA AAAGCCGCAT GTCCATGAAG CTACGTCGCT CCTCTGGCTC CGCCAATAAG 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014957494.1
CDS	213..7538
Translation	MFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQENKSENTTSFVGTPENIQFQKEPNSTVFTNISAPNQLDSFTKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDTTLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILSSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_015102008.1
Organism	Ovis aries(sheep)
Definition	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015102008.1

ORF Insert Sequence:

ATGTTTTCAT GCTTAATCCC TGGATTTTCA GCACCACAGT CTGAATATGG ACCTCGAACT 
TTAGATAACC TCATTAATCC TCCACTCAAC CTACAAGAAA CTCAAGTCAC TATAGAGGAA 
ATCACTCCAC TTGTCCCCCC ACAATCAGGA GATAAAGGAC AAGAAGATCT TACAAGCTAT 
TTTCTTGAAG CACTTTTAAA ATATATAGTA ATTCAGGTAA AAAGTTTAGA GTGGAAGAAC 
AAAGAAAACC AAGAAAAAGG ATTTTCATTT CTGTTTTCAC ATTTTAAGAA ATATTATTTG 
CCTTATATTT TCCCAAACAT CTGTAAGGAG AATAGTTTAT ATCATCCTGT ACTTGATATC 
CCACAGATGA GACCAAAGCC CCATTATGTC ATGATAAAGA AGGATGCTGA AACCAATGAG 
GCAATCTATT GTACAAAGGA GCCTTTCATT AAGGCTCGTG TTATTGTCAT TCGTTGGCTG 
GTTTCATTCT GGCTGGAGCC AAAGCCGCAT ACAGGACCTC ATATTCCTGG GATGGAAGGT 
GAAGTCTTGC CAAAAAATAT TCAGAGAGCA GCTGCTAGTT TGGTATCCAG AGAAGAAAAC 
AAAAATGATA ATGCTGATAA AACAGATAGA AGTACAGAAC CTGAACAGTC TCATTCCAAC 
ACAAGCACTC TCACGGAGCG AGAACCTAGC TCTTCTAGTC TCTGTAGTAT TGATGAAGAG 
CATCTCACAG ACATTGAAAT AGTTCGCCGA GTTTTTTCTT CTAAAAGAAG TAACGTAAAC 
TTTGTCACAG AGATATTTCG TCAGGCATTT TTATTACCAA TTTGCGAAGC AGCAGCTATG 
AGAAAAGTGG TAAAAGTATA TCAAGAATGG ATCCAGCAAG AGGAAAAACC TTTGTTCATG 
CAAGAGCCTG AAGAAATTGT GATCTCTTCT TCAGACCTTC CGTGCATTGA CAATGTCACA 
GACCATGATA TTTCAATGGA AGAAGGAGAA AAAAGAGAAG AGGAAAATGG GACCAATGTT 
GCTGATCATG TTCGAAATTC CACTTGGCCT AAAAATGGAA CCTACCAAGA TATTCTTCAT 
AATGCTTCTG AAGAAGCCAT AGAACAAAAC ATACGAGCTG GTGCCCAGGC AGTTTTGCAG 
GTGTTTATTA TAAATTCTTC AAACATATTT CTTCTTGAAC CTGCAAATGA GATAAAAAGT 
CTTCTGGATG AGCACACAGA TATGTGTAAA CGAATTCTTA ACATTTATCG GTTCATGGTT 
GTGCAAGTAT CAATGGACAA AAAGACTTGG GAACAGATGC TGCTTGTGTT GCTCAGAGTC 
ACGGAGTCTG TACTGAAGAT GCCATCACAA GCTTTTCTAC AGTTCCAGGG GAAAAAAAAC 
ATGACTTTGG CAGGTCGACT TGCAGGACCA CTTTTCCAGA CTCTTATAGT TGCCTGGATT 
AAAGCAAATC TAAATGTGTA CATCTCTCGA GAACTTTGGG ATGACTTACT CTCAGTATTG 
TCATCGTTGA CCTATTGGGA AGAGTTAGCC ACTGAATGGT CGCTAACTAT GGAGACACTA 
ACTAAAGTTT TAGCTAGAAA TTTGTATAGT TTGGATCTCA GTGATTTACC ATTGGATAAG 
CTGAGTGAAC AGAAACAAAA AAAGCACAAA GGGAAAGGAG TGGGACATGA ATTTCAGAAA 
GTTTCAGTTG ACAAGTCATT TTCTAGAGGA TGGAGTCGCG ATCAGCCTGG CCAAGCCCCA 
ATGAGGCAGA GAAGTGCAAC AACCACTGGT TCCCCAGGCA CAGAAAAGGC AAGGAGTATA 
GTACGGCAAA AAACTGTTGC CATGAGAAGC CGATCCATTG GTGAATGTGC TCTGCCATCG 
GCCTATATAC GCAGTGCTAA AAGTGCTCCT GTTCTGATCC ATACTTCCAA ACCCTTCTTG 
CCTGATATTG TTCTCACTCC CCTTTCTGAT GAGCTTTCAG ATATTGATGA TGCTCAAATA 
CTTCCTCGTC CAACTAGAGT CAGACATTTT TCGCAAAGTG AAGACACTGG AAATGAAGTT 
TTTGGTGCTT TGAGTGAGGA GCAGCCATTG CCTCGAAGTA GCAGCACTTC TGACATCTTG 
GAACCATTCA CTGTTGAACG AGCCAAAGGT GCAGTTCCTG TCATTGACAG TTCATCCCGT 
CATGCACCAA GCTTGCAGAG TTCCACAGAG GCTTCTTCAA TAACTAGATC CACTGAGAGC 
CACATCACTG ATACTCATAG TAGAGAGTCT TCTCTGGAAG TTGGTGATAG CATATATGAC 
CATCTCTGTC ACTTAATAGA TCCAGTAGAA CTTGCAGATT CAGCTTTTGA ACAAATCCAG 
TACATTGACC TTGAAGGAGA TGATGATCTT CTTTCCTCCC TGAAAGAATA TTTTAAGGAA 
ACCCAAGAAA GTCACAGTAA AAACGAGTTA GATAAAGATG CAGCTTCGCA GGAAGTGATC 
TCTGTTGCAG TAAATAAGGA TGAAAGATCA TCCTCAGATA AATTAGAGTA CATAGACCAG 
GAAAATAAAT CAGAAAATAC CACCTCTTTT GTTGGAACTC CTGAAAACAT ACAATTTCAA 
AAAGAACCAA ACTCTACTGT CTTCACAAAT ATTAGTGCAC CCAACCAGTT AGACAGCTTT 
ACGAAAACAA AAACTTCTGA AAAATGTAAA CAACTAAACA ATGATAAACA GTCATCGGAA 
CCTAGTTCAG GTAGCCCTTG TGATAAAGAA AAGAGAAAAC ATCTGTATAG ACAAACAGCC 
ACAGAATTAG ATGCCTGTAT AGATACCACA CTAGCTGAAA AGGTGAAGGG TGTGCAAATT 
AATGAAAAAA TCACTGTCCC ACATGTTTTG CATGCCAAGA AAACTACACT AAAAGCACCT 
GTTAACCGTA GAATGCCTCA TGTTACAAGC ACTAGCAAGC TTTCTCCAAC TAAAAGGAGC 
TTGTCTGAAA CAGTAACTCA CAGAGCCAAA ATCATGAAAA TTGCTACTAA AAAGCGAAAT 
AGTGTTCATG TTACTTTTAG ACCTTCTACT GAGTCAGTTC AGTTTTATAA TCCTCTGGAA 
AACAAAGAGG CTCCATGGAA GATGAGACTG CGGAAGCTCA GTGGCTTTAG TGGTAGTAAT 
AGCAACATCA GCAACCCCCA CACCAGCTCC AACAGTGCTG CAGAGCTAGT AAAACCTGGT 
GTGTACAGGC CTCTAGATAC AATTAGTGCA GCATCAGCAA GCAGTGAAAC AATTAAGGAA 
ACGACAGAGA TTCCCACCGC CATATTACAG AAAGAAGGAA TTGCAACTAA TCAGTTAGGT 
AGTCGTAGTA CTATTAAGTC ATCAAGTCAC GAGGCAGGAC TACCGCAGGG CTCCCTGGGT 
GGCGTTTATA AAACTGTTGT CCATGCTCTT TCGAAGCCGA AGGCAAATGT TTCCCCACAG 
AGGCAGAACA GAATGCCACC AGAGGCTCCA CTGAGGGATC TGTACAGTCA TGTAATGGGC 
TATTTTGGAA GGAAAGCTGC AGTCAATAAA GAGGACATGA GCACAAAACT GCCTCCTCTT 
AATAGTGATA TTGGCAGCAG CAATGCTAAT GTTCCTGATC TGATGGATGA GTTTATAGCA 
GAACGACTTC GAAGTGGTAA TGCCTCAACT ATGACACGAA GAGGAAGTAG TCCAGGTAGC 
CTCGAAATTC CCAAAGACCT CCCTGATATT CTAAATAAGC AAAACCAAAT GCGTCCTATT 
GATGACCCAG GTGTACCCTC AGAATGGACT TCTCCTGCCA GTGCAGGGAG CAGTGATCTT 
ATCAGCTCAG ATAGTCATTC GGATTCTTTC AGCGCTTTCC AGTATGATGG CCGAAAATTT 
GACAATTTTG GTTTTGGAGC TGATACTGGG AATGCATCCT CTGCTGACAT GGATTCAGGT 
TCTGGCCATT ATCAGAGTGC TGAAGAGCAA GAGGTGGCTA GTCTAACCAC ACTACACATT 
GACTCAGAAA CAAGCAGTCT TAATCAACAA GCTTTCTGTA CTGAAGTTGC AACGGTTACT 
GGTTCAGAAA GTGCCTCTCC AGTCCATTCA GCTCTGGGAT CCAGGTCACA GACACCTTCT 
CCTTCCACAC TGAATATAGA TCACATGGAA CAGAAGGATC TACAGCTCGA TGAGAAGCTC 
CACCACTCTG TTCTTCAGAC ACCAGATGAC CTAGAAATCA GTGAATTTCC ATCTGAATGC 
TGTAGTGTGA TGGCAGGGGG CACTCTTACA GGATGGCATG CTGATGTTGC TACTGTAATG 
TGGCGAAGGA TGCTGGGTAT TTTGGGAGAT GTCAACGCTA TTATGGATCC TGAAATACAT 
GCTCAAGTTT TTGATTACCT CTGTGAACTT TGGCAGAATC TAGCTAAGAT TAGAGATAAT 
CTTGGCATTT CAACTGATAA CCTGACCTCC CCTTCTCCAC CGGTTTTAAT TCCTCCACTG 
AGAATTCTTA CACCTTGGCT TTTTAAGGCA ACCATGTTAA CTGATAAATA TAAACAAGGT 
AAATTACATG CCTATAAACT TATTTGTAAT ACAATGAAAA GAAGACAAGA TGTTTCTCCA 
AATAGAGATT TTCTAACACA TTTCTACAAC ATAATGCATT GTGGATTGCT TCACATTGAT 
CAGGATATTG TCAATACAAT CATCAAGCAC TGCTCACCTC AATTTTTCTC ACTTGGTTTG 
CCTGGTGCAA CAATGCTTAT TATGGATTTT ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA 
GCTTTTCTCA ATGCACCAAG AGTGGAAGCA CAAGTTCTTC TGGGATCTTT GGTTTGCTTT 
CCCAACTTGT ATTGTGAACT GCCTGCTCTC CATCCCAACA TCCCTGATAT TGCTGTGTCT 
CAATTTACAG ATGTTAAGGA ACTTATAATC AAAACTGTGT TAAGCTCTGC AAGAGATGAG 
CCCTCTGGTC CTGCACGATG TGTAGCACTT TGCAGTTTAG GTATTTGGAT TTGTGAAGAA 
CTAGTCCATG AGTCTCATCA TCCTCAAATT AAGGAAGCTC TGAATGTAAT TTGTGTTTCC 
TTAAAGTTTA CTAATAAAAC AGTAGCCCAT GTAGCTTGTA ACATGCTTCA CATGCTGGTT 
CATTATGTAC CTAGACTTCA GATTTACCAG CCTGATTCTC CCTTGAAAAT TATTCAAATT 
CTAATAGCCA CTATTACCCA TCTTTTGCCA AGTACGGAAG CGTCATCTTA TGAAATGGAC 
AAGAGGTTGG TGGTATCCTT ACTTCTGTGT CTTCTGGACT GGATCATGGC CTTACCTCTT 
AAGACACTGC TCCAACCAGT CCATGCTACA GGAGCAGAAA ATGATAAAAT AGAAAAATCT 
GTTCTTAACT GCATTTATAA GGTATTACAT GGGTGTGTTT ATGGAGCTCA GTGTTTTAGC 
AATCCGAGGT ATTTTCCAAT AAGCCTTTCT GATTTGGCAT CTGTAGATTA TGATCCTTTT 
ATGCATTTGG AAAGCCTAAA AGAGCCTGAA CCTTTGCACT CTCCTGATTC GGAACGATCT 
TCTAAACTCC AGCCAGTAAC AGAAGTGAAA ACTCAGATGC AGCAAGGATT AATCTCCATA 
GCTGCCCGCA CTGTTATCAC ACATCTGGTG AATCACTTGG GCCATTATCC AATGAGCGGT 
GGTCCTGCTA TGTTAACAAG TCAGGTGTGT GAGAATCACG ACAATCATTA CAGTGAAAGC 
ACTGAACTTT CCCCTGAACT CTTCGAGAGT CCAAATATTC AGTTTTTTGT GTTAAACAAT 
ACAACCTTAG TGTCCTGTAT CCAGATCAGA GCTGAAGAGA GTATACCTGG AGGAGGTTTA 
TCTGCTGGTC TTGCATCAGC CAATTCAAAT GTCAGAATTA TAGTACGTGA TCTTTCTGGA 
AAATATTCAT GGGATTCTGC CATCCTGTAT GGACCACCTC TTGTAAGTGG TCTGTCAGAA 
CCTACATCTC TCGTACTTTC ATTGTCTCAC CAAGAGAAGC CAGAAGAACC TTCTGCATCT 
AATGAATGCT TAGAAGATAT AACAGTTAAA GATGGGATTT CCCTCCAGTT TAAAAGAAGT 
TTTAGAGAAA CTGTACCAAC TTGGGACACA ATCAGAGATG AAGAAGATGT TCTTGATGAG 
CTTTTGCAGT ATTTAGGTGT AACCAGTCCT GAATGCTTAC AGAGAACTGG AGTCTCACTT 
AATATTCCTG CTCCACAGCC TGTGTGCATT TCTGAAAAAC AGGAGAATGA TGTTATTAAT 
GCTATCCTTA AGCAGCATAC AGAGGAAAAA GAATTTGTTG AGAAACACTT TAATGACTTA 
AACATGAAAG CTGCAGAACA AGATGAACCA ACACCTCAGA AACCTCAGTC AGCATTTTAT 
TATTGCAGAT TGCTTCTTAG TATATTGGGA ATGAATTCCT GGGACAAAAG GAGGAGCTTT 
CATCTCCTGA AGAAAAATGA AAAACTACTT AGAGAACTTA GGAACTTGGA CTCAAGACAG 
TGCCGAGAGA CACACAAGAT TGCAGTATTT TATGTTGCTG AAGGACAAGA AGACAAACAT 
TCCATTCTCA CCAATACAGG AGGAAGTCAA GCATATGAAG ATTTTGTAGC TGGTCTTGGT 
TGGGAGGTAA ATCTTACAAA CCATTGTGGT TTCATGGGAG GACTACAAAA AAATAAAAGC 
ACTGGACTGA CCACACCGTA TTTTGCTACC TCTACAGTTG AAGTAATATT TCATGTGTCA 
ACAAGAATGC CTTCAGATTC TGATGACTCT TTGACCAAAA AATTGAGACA TTTAGGAAAT 
GATGAAGTGC ACATTGTTTG GTCAGAGCAT ACTAGAGACT ACAGGAGAGG AATTATTCCT 
ACAGAATTTG GTGATGTCCT TATTGTTATA TACCCGATGA AAAACCACAT GTTCAGTATC 
CAGATAATGA AAAAACCAGA GGTTCCCTTC TTTGGTCCAC TCTTTGATGG TGCTATTGTG 
AATGGAAAGG TCCTACCTAT TATGGTTCGA GCAACAGCTA TAAATGCAAG CCGTGCTCTG 
AAATCCCTGA TTCCATTGTA TCAAAACTTC TATGAGGAGA GAGCACGATA TCTGCAAACG 
ATTGTCCAGC ACCATTTAGA GCCAACGACA TTTGAAGATT TTGCAGCACA GGTTTTTTCT 
CCAGCTCCCT ACCATCATTT ACCATCTGAT GCCGTGGGCT CCTACCCAGA GATCCTATCC 
AGTGAAACTC CCACAGCGGC GCAGGTAGAC GGGGCCGACC TGGCCTCTCC AATGTCTCCT 
CGAACGAGCA AAAGCCGCAT GTCCATGAAG CTACGTCGCT CCTCTGGCTC CGCCAATAAG 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_012098817.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011954207.1 Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X10, mRNA.

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CloneID	OSm41103
Clone ID Related Accession (Same CDS sequence)	XM_012098817.2
Accession Version	XM_012098817.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	ral GTPase-activating protein subunit alpha-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012098817.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCC GGGGACAGGG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCCCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAACGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTAACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GCCATGAGAA GCCGATCCAT TGGTGAATGT 
GCTCTGCCAT CGGCCTATAT ACGCAGTGCT AAAAGTGCTC CTGTTCTGAT CCATACTTCC 
AAACCCTTCT TGCCTGATAT TGTTCTCACT CCCCTTTCTG ATGAGCTTTC AGATATTGAT 
GATGCTCAAA TACTTCCTCG TCCAACTAGA GTCAGACATT TTTCGCAAAG TGAAGACACT 
GGAAATGAAG TTTTTGGTGC TTTGAGTGAG GAGCAGCCAT TGCCTCGAAG TAGCAGCACT 
TCTGACATCT TGGAACCATT CACTGTTGAA CGAGCCAAAG GTGCAGTTCC TGTCATTGAC 
AGTTCATCCC GTCATGCACC AAGCTTGCAG AGTTCCACAG AGGCTTCTTC AATAACTAGA 
TCCACTGAGA GCCACATCAC TGATACTCAT AGTAGAGAGT CTTCTCTGGA AGTTGGTGAT 
AGCATATATG ACCATCTCTG TCACTTAATA GATCCAGTAG AACTTGCAGA TTCAGCTTTT 
GAACAAATCC AGTACATTGA CCTTGAAGGA GATGATGATC TTCTTTCCTC CCTGAAAGAA 
TATTTTAAGG AAACCCAAGA AAGTCACAGT AAAAACGAGT TAGATAAAGA TGCAGCTTCG 
CAGGAAGTGA TCTCTGTTGC AGTAAATAAG GATGAAAGAT CATCCTCAGA TAAATTAGAG 
TACATAGACC AGGAAAATAA ATCAGAAAAT ACCACCTCTT TTGTTGGAAC TCCTGAAAAC 
ATACAATTTC AAAAAGAACC AAACTCTACT GTCTTCACAA ATATTAGTGC ACCCAACCAG 
TTAGACAGCT TTACGAAAAC AAAAACTTCT GAAAAATGTA AACAACTAAA CAATGATAAA 
CAGTCATCGG AACCTAGTTC AGGTAGCCCT TGTGATAAAG AAAAGAGAAA ACATCTGTAT 
AGACAAACAG CCACAGAATT AGATGCCTGT ATAGATACCA CACTAGCTGA AAAGGTGAAG 
GGTGTGCAAA TTAATGAAAA AATCACTGTC CCACATGTTT TGCATGCCAA GAAAACTACA 
CTAAAAGCAC CTGTTAACCG TAGAATGCCT CATGTTACAA GCACTAGCAA GCTTTCTCCA 
ACTAAAAGGA GCTTGTCTGA AACAGTAACT CACAGAGCCA AAATCATGAA AATTGCTACT 
AAAAAGCGAA ATAGTGTTCA TGTTACTTTT AGACCTTCTA CTGAGTCAGT TCAGTTTTAT 
AATCCTCTGG AAAACAAAGA GGCTCCATGG AAGATGAGAC TGCGGAAGCT CAGTGGCTTT 
AGTGGTAGTA ATAGCAACAT CAGCAACCCC CACACCAGCT CCAACAGTGC TGCAGAGCTA 
GTAAAACCTG GTGTGTACAG GCCTCTAGAT ACAATTAGTG CAGCATCAGC AAGCAGTGAA 
ACAATTAAGG AAACGACAGA GATTCCCACC GCCATATTAC AGAAAGAAGG AATTGCAACT 
AATCAGTTAG GTAGTCGTAG TACTATTAAG TCATCAAGTC ACGAGGCAGG ACTACCGCAG 
GGCTCCCTGG GTGGCGTTTA TAAAACTGTT GTCCATGCTC TTTCGAAGCC GAAGGCAAAT 
GTTTCCCCAC AGAGGCAGAA CAGAATGCCA CCAGAGGCTC CACTGAGGGA TCTGTACAGT 
CATGTAATGG GCTATTTTGG AAGGAAAGCT GCAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAAGTAATT 
GAGACATTTA GGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011954207.1
CDS	457..7734
Translation	MFSKKPPGTGKKSTQKVLDTKKDALTRLKHLRIVIGRIFTAEPPEKPREHENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQENKSENTTSFVGTPENIQFQKEPNSTVFTNISAPNQLDSFTKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDTTLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKVIETFRK

Target ORF information:

RefSeq Version	XM_012098817.2
Organism	Ovis aries(sheep)
Definition	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012098817.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCC GGGGACAGGG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCCCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAACGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTAACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GCCATGAGAA GCCGATCCAT TGGTGAATGT 
GCTCTGCCAT CGGCCTATAT ACGCAGTGCT AAAAGTGCTC CTGTTCTGAT CCATACTTCC 
AAACCCTTCT TGCCTGATAT TGTTCTCACT CCCCTTTCTG ATGAGCTTTC AGATATTGAT 
GATGCTCAAA TACTTCCTCG TCCAACTAGA GTCAGACATT TTTCGCAAAG TGAAGACACT 
GGAAATGAAG TTTTTGGTGC TTTGAGTGAG GAGCAGCCAT TGCCTCGAAG TAGCAGCACT 
TCTGACATCT TGGAACCATT CACTGTTGAA CGAGCCAAAG GTGCAGTTCC TGTCATTGAC 
AGTTCATCCC GTCATGCACC AAGCTTGCAG AGTTCCACAG AGGCTTCTTC AATAACTAGA 
TCCACTGAGA GCCACATCAC TGATACTCAT AGTAGAGAGT CTTCTCTGGA AGTTGGTGAT 
AGCATATATG ACCATCTCTG TCACTTAATA GATCCAGTAG AACTTGCAGA TTCAGCTTTT 
GAACAAATCC AGTACATTGA CCTTGAAGGA GATGATGATC TTCTTTCCTC CCTGAAAGAA 
TATTTTAAGG AAACCCAAGA AAGTCACAGT AAAAACGAGT TAGATAAAGA TGCAGCTTCG 
CAGGAAGTGA TCTCTGTTGC AGTAAATAAG GATGAAAGAT CATCCTCAGA TAAATTAGAG 
TACATAGACC AGGAAAATAA ATCAGAAAAT ACCACCTCTT TTGTTGGAAC TCCTGAAAAC 
ATACAATTTC AAAAAGAACC AAACTCTACT GTCTTCACAA ATATTAGTGC ACCCAACCAG 
TTAGACAGCT TTACGAAAAC AAAAACTTCT GAAAAATGTA AACAACTAAA CAATGATAAA 
CAGTCATCGG AACCTAGTTC AGGTAGCCCT TGTGATAAAG AAAAGAGAAA ACATCTGTAT 
AGACAAACAG CCACAGAATT AGATGCCTGT ATAGATACCA CACTAGCTGA AAAGGTGAAG 
GGTGTGCAAA TTAATGAAAA AATCACTGTC CCACATGTTT TGCATGCCAA GAAAACTACA 
CTAAAAGCAC CTGTTAACCG TAGAATGCCT CATGTTACAA GCACTAGCAA GCTTTCTCCA 
ACTAAAAGGA GCTTGTCTGA AACAGTAACT CACAGAGCCA AAATCATGAA AATTGCTACT 
AAAAAGCGAA ATAGTGTTCA TGTTACTTTT AGACCTTCTA CTGAGTCAGT TCAGTTTTAT 
AATCCTCTGG AAAACAAAGA GGCTCCATGG AAGATGAGAC TGCGGAAGCT CAGTGGCTTT 
AGTGGTAGTA ATAGCAACAT CAGCAACCCC CACACCAGCT CCAACAGTGC TGCAGAGCTA 
GTAAAACCTG GTGTGTACAG GCCTCTAGAT ACAATTAGTG CAGCATCAGC AAGCAGTGAA 
ACAATTAAGG AAACGACAGA GATTCCCACC GCCATATTAC AGAAAGAAGG AATTGCAACT 
AATCAGTTAG GTAGTCGTAG TACTATTAAG TCATCAAGTC ACGAGGCAGG ACTACCGCAG 
GGCTCCCTGG GTGGCGTTTA TAAAACTGTT GTCCATGCTC TTTCGAAGCC GAAGGCAAAT 
GTTTCCCCAC AGAGGCAGAA CAGAATGCCA CCAGAGGCTC CACTGAGGGA TCTGTACAGT 
CATGTAATGG GCTATTTTGG AAGGAAAGCT GCAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAAGTAATT 
GAGACATTTA GGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_012098818.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011954208.1 Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X1, mRNA.

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CloneID	OSm41104
Clone ID Related Accession (Same CDS sequence)	XM_012098818.1
Accession Version	XM_012098818.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	ral GTPase-activating protein subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAAG TGGTAAAAGT ATATCAAGAA TGGATCCAGC AAGAGGAAAA ACCTTTGTTC 
ATGCAAGAGC CTGAAGAAAT TGTGATCTCT TCTTCAGACC TTCCGTGCAT TGACAATGTC 
ACAGACCATG ATATTTCAAT GGAAGAAGGA GAAAAAAGAG AAGAGGAAAA TGGGACCAAT 
GTTGCTGATC ATGTTCGAAA TTCCACTTGG CCTAAAAATG GAACCTACCA AGATATTCTT 
CATAATGCTT CTGAAGAAGC CATAGAACAA AACATACGAG CTGGTGCCCA GGCAGTTTTG 
CAGGTGTTTA TTATAAATTC TTCAAACATA TTTCTTCTTG AACCTGCAAA TGAGATAAAA 
AGTCTTCTGG ATGAGCACAC AGATATGTGT AAACGAATTC TTAACATTTA TCGGTTCATG 
GTTGTGCAAG TATCAATGGA CAAAAAGACT TGGGAACAGA TGCTGCTTGT GTTGCTCAGA 
GTCACGGAGT CTGTACTGAA GATGCCATCA CAAGCTTTTC TACAGTTCCA GGGGAAAAAA 
AACATGACTT TGGCAGGTCG ACTTGCAGGA CCACTTTTCC AGACTCTTAT AGTTGCCTGG 
ATTAAAGCAA ATCTAAATGT GTACATCTCT CGAGAACTTT GGGATGACTT ACTCTCAGTA 
TTGTCATCGT TGACCTATTG GGAAGAGTTA GCCACTGAAT GGTCGCTAAC TATGGAGACA 
CTAACTAAAG TTTTAGCTAG AAATTTGTAT AGTTTGGATC TCAGTGATTT ACCATTGGAT 
AAGCTGAGTG AACAGAAACA AAAAAAGCAC AAAGGGAAAG GAGTGGGACA TGAATTTCAG 
AAAGTTTCAG TTGACAAGTC ATTTTCTAGA GGATGGAGTC GCGATCAGCC TGGCCAAGCC 
CCAATGAGGC AGAGAAGTGC AACAACCACT GGTTCCCCAG GCACAGAAAA GGCAAGGAGT 
ATAGTACGGC AAAAAACTGT TGCCATGAGA AGCCGATCCA TTGGTGAATG TGCTCTGCCA 
TCGGCCTATA TACGCAGTGC TAAAAGTGCT CCTGTTCTGA TCCATACTTC CAAACCCTTC 
TTGCCTGATA TTGTTCTCAC TCCCCTTTCT GATGAGCTTT CAGATATTGA TGATGCTCAA 
ATACTTCCTC GTCCAACTAG AGTCAGACAT TTTTCGCAAA GTGAAGACAC TGGAAATGAA 
GTTTTTGGTG CTTTGAGTGA GGAGCAGCCA TTGCCTCGAA GTAGCAGCAC TTCTGACATC 
TTGGAACCAT TCACTGTTGA ACGAGCCAAA GGTGCAGTTC CTGTCATTGA CAGTTCATCC 
CGTCATGCAC CAAGCTTGCA GAGTTCCACA GAGGCTTCTT CAATAACTAG ATCCACTGAG 
AGCCACATCA CTGATACTCA TAGTAGAGAG TCTTCTCTGG AAGTTGGTGA TAGCATATAT 
GACCATCTCT GTCACTTAAT AGATCCAGTA GAACTTGCAG ATTCAGCTTT TGAACAAATC 
CAGTACATTG ACCTTGAAGG AGATGATGAT CTTCTTTCCT CCCTGAAAGA ATATTTTAAG 
GAAACCCAAG AAAGTCACAG TAAAAACGAG TTAGATAAAG ATGCAGCTTC GCAGGAAGTG 
ATCTCTGTTG CAGTAAATAA GGATGAAAGA TCATCCTCAG ATAAATTAGA GTACATAGAC 
CAGGAAAATA AATCAGAAAA TACCACCTCT TTTGTTGGAA CTCCTGAAAA CATACAATTT 
CAAAAAGAAC CAAACTCTAC TGTCTTCACA AATATTAGTG CACCCAACCA GTTAGACAGC 
TTTACGAAAA CAAAAACTTC TGAAAAATGT AAACAACTAA ACAATGATAA ACAGTCATCG 
GAACCTAGTT CAGGTAGCCC TTGTGATAAA GAAAAGAGAA AACATCTGTA TAGACAAACA 
GCCACAGAAT TAGATGCCTG TATAGATACC ACACTAGCTG AAAAGGTGAA GGGTGTGCAA 
ATTAATGAAA AAATCACTGT CCCACATGTT TTGCATGCCA AGAAAACTAC ACTAAAAGCA 
CCTGTTAACC GTAGAATGCC TCATGTTACA AGCACTAGCA AGCTTTCTCC AACTAAAAGG 
AGCTTGTCTG AAACAGTAAC TCACAGAGCC AAAATCATGA AAATTGCTAC TAAAAAGCGA 
AATAGTGTTC ATGTTACTTT TAGACCTTCT ACTGAGTCAG TTCAGTTTTA TAATCCTCTG 
GAAAACAAAG AGGCTCCATG GAAGATGAGA CTGCGGAAGC TCAGTGGCTT TAGTGGTAGT 
AATAGCAACA TCAGCAACCC CCACACCAGC TCCAACAGTG CTGCAGAGCT AGTAAAACCT 
GGTGTGTACA GGCCTCTAGA TACAATTAGT GCAGCATCAG CAAGCAGTGA AACAATTAAG 
GAAACGACAG AGATTCCCAC CGCCATATTA CAGAAAGAAG GAATTGCAAC TAATCAGTTA 
GGTAGTCGTA GTACTATTAA GTCATCAAGT CACGAGGCAG GACTACCGCA GGGCTCCCTG 
GGTGGCGTTT ATAAAACTGT TGTCCATGCT CTTTCGAAGC CGAAGGCAAA TGTTTCCCCA 
CAGAGGCAGA ACAGAATGCC ACCAGAGGCT CCACTGAGGG ATCTGTACAG TCATGTAATG 
GGCTATTTTG GAAGGAAAGC TGCAGTCAAT AAAGAGGACA TGAGCACAAA ACTGCCTCCT 
CTTAATAGTG ATATTGGCAG CAGCAATGCT AATGTTCCTG ATCTGATGGA TGAGTTTATA 
GCAGAACGAC TTCGAAGTGG TAATGCCTCA ACTATGACAC GAAGAGGAAG TAGTCCAGGT 
AGCCTCGAAA TTCCCAAAGA CCTCCCTGAT ATTCTAAATA AGCAAAACCA AATGCGTCCT 
ATTGATGACC CAGGTGTACC CTCAGAATGG ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT 
CTTATCAGCT CAGATAGTCA TTCGGATTCT TTCAGCGCTT TCCAGTATGA TGGCCGAAAA 
TTTGACAATT TTGGTTTTGG AGCTGATACT GGGAATGCAT CCTCTGCTGA CATGGATTCA 
GGTTCTGGCC ATTATCAGAG TGCTGAAGAG CAAGAGGTGG CTAGTCTAAC CACACTACAC 
ATTGACTCAG AAACAAGCAG TCTTAATCAA CAAGCTTTCT GTACTGAAGT TGCAACGGTT 
ACTGGTTCAG AAAGTGCCTC TCCAGTCCAT TCAGCTCTGG GATCCAGGTC ACAGACACCT 
TCTCCTTCCA CACTGAATAT AGATCACATG GAACAGAAGG ATCTACAGCT CGATGAGAAG 
CTCCACCACT CTGTTCTTCA GACACCAGAT GACCTAGAAA TCAGTGAATT TCCATCTGAA 
TGCTGTAGTG TGATGGCAGG GGGCACTCTT ACAGGATGGC ATGCTGATGT TGCTACTGTA 
ATGTGGCGAA GGATGCTGGG TATTTTGGGA GATGTCAACG CTATTATGGA TCCTGAAATA 
CATGCTCAAG TTTTTGATTA CCTCTGTGAA CTTTGGCAGA ATCTAGCTAA GATTAGAGAT 
AATCTTGGCA TTTCAACTGA TAACCTGACC TCCCCTTCTC CACCGGTTTT AATTCCTCCA 
CTGAGAATTC TTACACCTTG GCTTTTTAAG GCAACCATGT TAACTGATAA ATATAAACAA 
GGTAAATTAC ATGCCTATAA ACTTATTTGT AATACAATGA AAAGAAGACA AGATGTTTCT 
CCAAATAGAG ATTTTCTAAC ACATTTCTAC AACATAATGC ATTGTGGATT GCTTCACATT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCAC CTCAATTTTT CTCACTTGGT 
TTGCCTGGTG CAACAATGCT TATTATGGAT TTTATTGTAG CAGCTGGTAG AGTGGCTTCT 
TCAGCTTTTC TCAATGCACC AAGAGTGGAA GCACAAGTTC TTCTGGGATC TTTGGTTTGC 
TTTCCCAACT TGTATTGTGA ACTGCCTGCT CTCCATCCCA ACATCCCTGA TATTGCTGTG 
TCTCAATTTA CAGATGTTAA GGAACTTATA ATCAAAACTG TGTTAAGCTC TGCAAGAGAT 
GAGCCCTCTG GTCCTGCACG ATGTGTAGCA CTTTGCAGTT TAGGTATTTG GATTTGTGAA 
GAACTAGTCC ATGAGTCTCA TCATCCTCAA ATTAAGGAAG CTCTGAATGT AATTTGTGTT 
TCCTTAAAGT TTACTAATAA AACAGTAGCC CATGTAGCTT GTAACATGCT TCACATGCTG 
GTTCATTATG TACCTAGACT TCAGATTTAC CAGCCTGATT CTCCCTTGAA AATTATTCAA 
ATTCTAATAG CCACTATTAC CCATCTTTTG CCAAGTACGG AAGCGTCATC TTATGAAATG 
GACAAGAGGT TGGTGGTATC CTTACTTCTG TGTCTTCTGG ACTGGATCAT GGCCTTACCT 
CTTAAGACAC TGCTCCAACC AGTCCATGCT ACAGGAGCAG AAAATGATAA AATAGAAAAA 
TCTGTTCTTA ACTGCATTTA TAAGGTATTA CATGGGTGTG TTTATGGAGC TCAGTGTTTT 
AGCAATCCGA GGTATTTTCC AATAAGCCTT TCTGATTTGG CATCTGTAGA TTATGATCCT 
TTTATGCATT TGGAAAGCCT AAAAGAGCCT GAACCTTTGC ACTCTCCTGA TTCGGAACGA 
TCTTCTAAAC TCCAGCCAGT AACAGAAGTG AAAACTCAGA TGCAGCAAGG ATTAATCTCC 
ATAGCTGCCC GCACTGTTAT CACACATCTG GTGAATCACT TGGGCCATTA TCCAATGAGC 
GGTGGTCCTG CTATGTTAAC AAGTCAGGTG TGTGAGAATC ACGACAATCA TTACAGTGAA 
AGCACTGAAC TTTCCCCTGA ACTCTTCGAG AGTCCAAATA TTCAGTTTTT TGTGTTAAAC 
AATACAACCT TAGTGTCCTG TATCCAGATC AGAGCTGAAG AGAGTATACC TGGAGGAGGT 
TTATCTGCTG GTCTTGCATC AGCCAATTCA AATGTCAGAA TTATAGTACG TGATCTTTCT 
GGAAAATATT CATGGGATTC TGCCATCCTG TATGGACCAC CTCTTGTAAG TGGTCTGTCA 
GAACCTACAT CTCTCGTACT TTCATTGTCT CACCAAGAGA AGCCAGAAGA ACCTTCTGCA 
TCTAATGAAT GCTTAGAAGA TATAACAGTT AAAGATGGGA TTTCCCTCCA GTTTAAAAGA 
AGTTTTAGAG AAACTGTACC AACTTGGGAC ACAATCAGAG ATGAAGAAGA TGTTCTTGAT 
GAGCTTTTGC AGTATTTAGG TGTAACCAGT CCTGAATGCT TACAGAGAAC TGGAGTCTCA 
CTTAATATTC CTGCTCCACA GCCTGTGTGC ATTTCTGAAA AACAGGAGAA TGATGTTATT 
AATGCTATCC TTAAGCAGCA TACAGAGGAA AAAGAATTTG TTGAGAAACA CTTTAATGAC 
TTAAACATGA AAGCTGCAGA ACAAGATGAA CCAACACCTC AGAAACCTCA GTCAGCATTT 
TATTATTGCA GATTGCTTCT TAGTATATTG GGAATGAATT CCTGGGACAA AAGGAGGAGC 
TTTCATCTCC TGAAGAAAAA TGAAAAACTA CTTAGAGAAC TTAGGAACTT GGACTCAAGA 
CAGTGCCGAG AGACACACAA GATTGCAGTA TTTTATGTTG CTGAAGGACA AGAAGACAAA 
CATTCCATTC TCACCAATAC AGGAGGAAGT CAAGCATATG AAGATTTTGT AGCTGGTCTT 
GGTTGGGAGG TAAATCTTAC AAACCATTGT GGTTTCATGG GAGGACTACA AAAAAATAAA 
AGCACTGGAC TGACCACACC GTATTTTGCT ACCTCTACAG TTGAAGTAAT ATTTCATGTG 
TCAACAAGAA TGCCTTCAGA TTCTGATGAC TCTTTGACCA AAAAATTGAG ACATTTAGGA 
AATGATGAAG TGCACATTGT TTGGTCAGAG CATACTAGAG ACTACAGGAG AGGAATTATT 
CCTACAGAAT TTGGTGATGT CCTTATTGTT ATATACCCGA TGAAAAACCA CATGTTCAGT 
ATCCAGATAA TGAAAAAACC AGAGGTTCCC TTCTTTGGTC CACTCTTTGA TGGTGCTATT 
GTGAATGGAA AGGTCCTACC TATTATGGTT CGAGCAACAG CTATAAATGC AAGCCGTGCT 
CTGAAATCCC TGATTCCATT GTATCAAAAC TTCTATGAGG AGAGAGCACG ATATCTGCAA 
ACGATTGTCC AGCACCATTT AGAGCCAACG ACATTTGAAG ATTTTGCAGC ACAGGTTTTT 
TCTCCAGCTC CCTACCATCA TTTACCATCT GATGCCGTGG GCTCCTACCC AGAGATCCTA 
TCCAGTGAAA CTCCCACAGC GGCGCAGGTA GACGGGGCCG ACCTGGCCTC TCCAATGTCT 
CCTCGAACGA GCAAAAGCCG CATGTCCATG AAGCTACGTC GCTCCTCTGG CTCCGCCAAT 
AAGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011954208.1
CDS	172..6660
Translation	MRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQENKSENTTSFVGTPENIQFQKEPNSTVFTNISAPNQLDSFTKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDTTLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILSSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_012098818.1
Organism	Ovis aries(sheep)
Definition	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012098818.1

ORF Insert Sequence:

ATGAGAAAAG TGGTAAAAGT ATATCAAGAA TGGATCCAGC AAGAGGAAAA ACCTTTGTTC 
ATGCAAGAGC CTGAAGAAAT TGTGATCTCT TCTTCAGACC TTCCGTGCAT TGACAATGTC 
ACAGACCATG ATATTTCAAT GGAAGAAGGA GAAAAAAGAG AAGAGGAAAA TGGGACCAAT 
GTTGCTGATC ATGTTCGAAA TTCCACTTGG CCTAAAAATG GAACCTACCA AGATATTCTT 
CATAATGCTT CTGAAGAAGC CATAGAACAA AACATACGAG CTGGTGCCCA GGCAGTTTTG 
CAGGTGTTTA TTATAAATTC TTCAAACATA TTTCTTCTTG AACCTGCAAA TGAGATAAAA 
AGTCTTCTGG ATGAGCACAC AGATATGTGT AAACGAATTC TTAACATTTA TCGGTTCATG 
GTTGTGCAAG TATCAATGGA CAAAAAGACT TGGGAACAGA TGCTGCTTGT GTTGCTCAGA 
GTCACGGAGT CTGTACTGAA GATGCCATCA CAAGCTTTTC TACAGTTCCA GGGGAAAAAA 
AACATGACTT TGGCAGGTCG ACTTGCAGGA CCACTTTTCC AGACTCTTAT AGTTGCCTGG 
ATTAAAGCAA ATCTAAATGT GTACATCTCT CGAGAACTTT GGGATGACTT ACTCTCAGTA 
TTGTCATCGT TGACCTATTG GGAAGAGTTA GCCACTGAAT GGTCGCTAAC TATGGAGACA 
CTAACTAAAG TTTTAGCTAG AAATTTGTAT AGTTTGGATC TCAGTGATTT ACCATTGGAT 
AAGCTGAGTG AACAGAAACA AAAAAAGCAC AAAGGGAAAG GAGTGGGACA TGAATTTCAG 
AAAGTTTCAG TTGACAAGTC ATTTTCTAGA GGATGGAGTC GCGATCAGCC TGGCCAAGCC 
CCAATGAGGC AGAGAAGTGC AACAACCACT GGTTCCCCAG GCACAGAAAA GGCAAGGAGT 
ATAGTACGGC AAAAAACTGT TGCCATGAGA AGCCGATCCA TTGGTGAATG TGCTCTGCCA 
TCGGCCTATA TACGCAGTGC TAAAAGTGCT CCTGTTCTGA TCCATACTTC CAAACCCTTC 
TTGCCTGATA TTGTTCTCAC TCCCCTTTCT GATGAGCTTT CAGATATTGA TGATGCTCAA 
ATACTTCCTC GTCCAACTAG AGTCAGACAT TTTTCGCAAA GTGAAGACAC TGGAAATGAA 
GTTTTTGGTG CTTTGAGTGA GGAGCAGCCA TTGCCTCGAA GTAGCAGCAC TTCTGACATC 
TTGGAACCAT TCACTGTTGA ACGAGCCAAA GGTGCAGTTC CTGTCATTGA CAGTTCATCC 
CGTCATGCAC CAAGCTTGCA GAGTTCCACA GAGGCTTCTT CAATAACTAG ATCCACTGAG 
AGCCACATCA CTGATACTCA TAGTAGAGAG TCTTCTCTGG AAGTTGGTGA TAGCATATAT 
GACCATCTCT GTCACTTAAT AGATCCAGTA GAACTTGCAG ATTCAGCTTT TGAACAAATC 
CAGTACATTG ACCTTGAAGG AGATGATGAT CTTCTTTCCT CCCTGAAAGA ATATTTTAAG 
GAAACCCAAG AAAGTCACAG TAAAAACGAG TTAGATAAAG ATGCAGCTTC GCAGGAAGTG 
ATCTCTGTTG CAGTAAATAA GGATGAAAGA TCATCCTCAG ATAAATTAGA GTACATAGAC 
CAGGAAAATA AATCAGAAAA TACCACCTCT TTTGTTGGAA CTCCTGAAAA CATACAATTT 
CAAAAAGAAC CAAACTCTAC TGTCTTCACA AATATTAGTG CACCCAACCA GTTAGACAGC 
TTTACGAAAA CAAAAACTTC TGAAAAATGT AAACAACTAA ACAATGATAA ACAGTCATCG 
GAACCTAGTT CAGGTAGCCC TTGTGATAAA GAAAAGAGAA AACATCTGTA TAGACAAACA 
GCCACAGAAT TAGATGCCTG TATAGATACC ACACTAGCTG AAAAGGTGAA GGGTGTGCAA 
ATTAATGAAA AAATCACTGT CCCACATGTT TTGCATGCCA AGAAAACTAC ACTAAAAGCA 
CCTGTTAACC GTAGAATGCC TCATGTTACA AGCACTAGCA AGCTTTCTCC AACTAAAAGG 
AGCTTGTCTG AAACAGTAAC TCACAGAGCC AAAATCATGA AAATTGCTAC TAAAAAGCGA 
AATAGTGTTC ATGTTACTTT TAGACCTTCT ACTGAGTCAG TTCAGTTTTA TAATCCTCTG 
GAAAACAAAG AGGCTCCATG GAAGATGAGA CTGCGGAAGC TCAGTGGCTT TAGTGGTAGT 
AATAGCAACA TCAGCAACCC CCACACCAGC TCCAACAGTG CTGCAGAGCT AGTAAAACCT 
GGTGTGTACA GGCCTCTAGA TACAATTAGT GCAGCATCAG CAAGCAGTGA AACAATTAAG 
GAAACGACAG AGATTCCCAC CGCCATATTA CAGAAAGAAG GAATTGCAAC TAATCAGTTA 
GGTAGTCGTA GTACTATTAA GTCATCAAGT CACGAGGCAG GACTACCGCA GGGCTCCCTG 
GGTGGCGTTT ATAAAACTGT TGTCCATGCT CTTTCGAAGC CGAAGGCAAA TGTTTCCCCA 
CAGAGGCAGA ACAGAATGCC ACCAGAGGCT CCACTGAGGG ATCTGTACAG TCATGTAATG 
GGCTATTTTG GAAGGAAAGC TGCAGTCAAT AAAGAGGACA TGAGCACAAA ACTGCCTCCT 
CTTAATAGTG ATATTGGCAG CAGCAATGCT AATGTTCCTG ATCTGATGGA TGAGTTTATA 
GCAGAACGAC TTCGAAGTGG TAATGCCTCA ACTATGACAC GAAGAGGAAG TAGTCCAGGT 
AGCCTCGAAA TTCCCAAAGA CCTCCCTGAT ATTCTAAATA AGCAAAACCA AATGCGTCCT 
ATTGATGACC CAGGTGTACC CTCAGAATGG ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT 
CTTATCAGCT CAGATAGTCA TTCGGATTCT TTCAGCGCTT TCCAGTATGA TGGCCGAAAA 
TTTGACAATT TTGGTTTTGG AGCTGATACT GGGAATGCAT CCTCTGCTGA CATGGATTCA 
GGTTCTGGCC ATTATCAGAG TGCTGAAGAG CAAGAGGTGG CTAGTCTAAC CACACTACAC 
ATTGACTCAG AAACAAGCAG TCTTAATCAA CAAGCTTTCT GTACTGAAGT TGCAACGGTT 
ACTGGTTCAG AAAGTGCCTC TCCAGTCCAT TCAGCTCTGG GATCCAGGTC ACAGACACCT 
TCTCCTTCCA CACTGAATAT AGATCACATG GAACAGAAGG ATCTACAGCT CGATGAGAAG 
CTCCACCACT CTGTTCTTCA GACACCAGAT GACCTAGAAA TCAGTGAATT TCCATCTGAA 
TGCTGTAGTG TGATGGCAGG GGGCACTCTT ACAGGATGGC ATGCTGATGT TGCTACTGTA 
ATGTGGCGAA GGATGCTGGG TATTTTGGGA GATGTCAACG CTATTATGGA TCCTGAAATA 
CATGCTCAAG TTTTTGATTA CCTCTGTGAA CTTTGGCAGA ATCTAGCTAA GATTAGAGAT 
AATCTTGGCA TTTCAACTGA TAACCTGACC TCCCCTTCTC CACCGGTTTT AATTCCTCCA 
CTGAGAATTC TTACACCTTG GCTTTTTAAG GCAACCATGT TAACTGATAA ATATAAACAA 
GGTAAATTAC ATGCCTATAA ACTTATTTGT AATACAATGA AAAGAAGACA AGATGTTTCT 
CCAAATAGAG ATTTTCTAAC ACATTTCTAC AACATAATGC ATTGTGGATT GCTTCACATT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCAC CTCAATTTTT CTCACTTGGT 
TTGCCTGGTG CAACAATGCT TATTATGGAT TTTATTGTAG CAGCTGGTAG AGTGGCTTCT 
TCAGCTTTTC TCAATGCACC AAGAGTGGAA GCACAAGTTC TTCTGGGATC TTTGGTTTGC 
TTTCCCAACT TGTATTGTGA ACTGCCTGCT CTCCATCCCA ACATCCCTGA TATTGCTGTG 
TCTCAATTTA CAGATGTTAA GGAACTTATA ATCAAAACTG TGTTAAGCTC TGCAAGAGAT 
GAGCCCTCTG GTCCTGCACG ATGTGTAGCA CTTTGCAGTT TAGGTATTTG GATTTGTGAA 
GAACTAGTCC ATGAGTCTCA TCATCCTCAA ATTAAGGAAG CTCTGAATGT AATTTGTGTT 
TCCTTAAAGT TTACTAATAA AACAGTAGCC CATGTAGCTT GTAACATGCT TCACATGCTG 
GTTCATTATG TACCTAGACT TCAGATTTAC CAGCCTGATT CTCCCTTGAA AATTATTCAA 
ATTCTAATAG CCACTATTAC CCATCTTTTG CCAAGTACGG AAGCGTCATC TTATGAAATG 
GACAAGAGGT TGGTGGTATC CTTACTTCTG TGTCTTCTGG ACTGGATCAT GGCCTTACCT 
CTTAAGACAC TGCTCCAACC AGTCCATGCT ACAGGAGCAG AAAATGATAA AATAGAAAAA 
TCTGTTCTTA ACTGCATTTA TAAGGTATTA CATGGGTGTG TTTATGGAGC TCAGTGTTTT 
AGCAATCCGA GGTATTTTCC AATAAGCCTT TCTGATTTGG CATCTGTAGA TTATGATCCT 
TTTATGCATT TGGAAAGCCT AAAAGAGCCT GAACCTTTGC ACTCTCCTGA TTCGGAACGA 
TCTTCTAAAC TCCAGCCAGT AACAGAAGTG AAAACTCAGA TGCAGCAAGG ATTAATCTCC 
ATAGCTGCCC GCACTGTTAT CACACATCTG GTGAATCACT TGGGCCATTA TCCAATGAGC 
GGTGGTCCTG CTATGTTAAC AAGTCAGGTG TGTGAGAATC ACGACAATCA TTACAGTGAA 
AGCACTGAAC TTTCCCCTGA ACTCTTCGAG AGTCCAAATA TTCAGTTTTT TGTGTTAAAC 
AATACAACCT TAGTGTCCTG TATCCAGATC AGAGCTGAAG AGAGTATACC TGGAGGAGGT 
TTATCTGCTG GTCTTGCATC AGCCAATTCA AATGTCAGAA TTATAGTACG TGATCTTTCT 
GGAAAATATT CATGGGATTC TGCCATCCTG TATGGACCAC CTCTTGTAAG TGGTCTGTCA 
GAACCTACAT CTCTCGTACT TTCATTGTCT CACCAAGAGA AGCCAGAAGA ACCTTCTGCA 
TCTAATGAAT GCTTAGAAGA TATAACAGTT AAAGATGGGA TTTCCCTCCA GTTTAAAAGA 
AGTTTTAGAG AAACTGTACC AACTTGGGAC ACAATCAGAG ATGAAGAAGA TGTTCTTGAT 
GAGCTTTTGC AGTATTTAGG TGTAACCAGT CCTGAATGCT TACAGAGAAC TGGAGTCTCA 
CTTAATATTC CTGCTCCACA GCCTGTGTGC ATTTCTGAAA AACAGGAGAA TGATGTTATT 
AATGCTATCC TTAAGCAGCA TACAGAGGAA AAAGAATTTG TTGAGAAACA CTTTAATGAC 
TTAAACATGA AAGCTGCAGA ACAAGATGAA CCAACACCTC AGAAACCTCA GTCAGCATTT 
TATTATTGCA GATTGCTTCT TAGTATATTG GGAATGAATT CCTGGGACAA AAGGAGGAGC 
TTTCATCTCC TGAAGAAAAA TGAAAAACTA CTTAGAGAAC TTAGGAACTT GGACTCAAGA 
CAGTGCCGAG AGACACACAA GATTGCAGTA TTTTATGTTG CTGAAGGACA AGAAGACAAA 
CATTCCATTC TCACCAATAC AGGAGGAAGT CAAGCATATG AAGATTTTGT AGCTGGTCTT 
GGTTGGGAGG TAAATCTTAC AAACCATTGT GGTTTCATGG GAGGACTACA AAAAAATAAA 
AGCACTGGAC TGACCACACC GTATTTTGCT ACCTCTACAG TTGAAGTAAT ATTTCATGTG 
TCAACAAGAA TGCCTTCAGA TTCTGATGAC TCTTTGACCA AAAAATTGAG ACATTTAGGA 
AATGATGAAG TGCACATTGT TTGGTCAGAG CATACTAGAG ACTACAGGAG AGGAATTATT 
CCTACAGAAT TTGGTGATGT CCTTATTGTT ATATACCCGA TGAAAAACCA CATGTTCAGT 
ATCCAGATAA TGAAAAAACC AGAGGTTCCC TTCTTTGGTC CACTCTTTGA TGGTGCTATT 
GTGAATGGAA AGGTCCTACC TATTATGGTT CGAGCAACAG CTATAAATGC AAGCCGTGCT 
CTGAAATCCC TGATTCCATT GTATCAAAAC TTCTATGAGG AGAGAGCACG ATATCTGCAA 
ACGATTGTCC AGCACCATTT AGAGCCAACG ACATTTGAAG ATTTTGCAGC ACAGGTTTTT 
TCTCCAGCTC CCTACCATCA TTTACCATCT GATGCCGTGG GCTCCTACCC AGAGATCCTA 
TCCAGTGAAA CTCCCACAGC GGCGCAGGTA GACGGGGCCG ACCTGGCCTC TCCAATGTCT 
CCTCGAACGA GCAAAAGCCG CATGTCCATG AAGCTACGTC GCTCCTCTGG CTCCGCCAAT 
AAGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_012098819.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011954209.1 Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X11, mRNA.

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CloneID	OSm41105
Clone ID Related Accession (Same CDS sequence)	XM_012098819.2
Accession Version	XM_012098819.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	ral GTPase-activating protein subunit alpha-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012098819.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCC GGGGACAGGG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCCCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAACGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTAACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GCCATGAGAA GCCGATCCAT TGGTGAATGT 
GCTCTGCCAT CGGCCTATAT ACGCAGTGCT AAAAGTGCTC CTGTTCTGAT CCATACTTCC 
AAACCCTTCT TGCCTGATAT TGTTCTCACT CCCCTTTCTG ATGAGCTTTC AGATATTGAT 
GATGCTCAAA TACTTCCTCG TCCAACTAGA GTCAGACATT TTTCGCAAAG TGAAGACACT 
GGAAATGAAG TTTTTGGTGC TTTGAGTGAG GAGCAGCCAT TGCCTCGAAG TAGCAGCACT 
TCTGACATCT TGGAACCATT CACTGTTGAA CGAGCCAAAG TCAATAAAGA GGACATGAGC 
ACAAAACTGC CTCCTCTTAA TAGTGATATT GGCAGCAGCA ATGCTAATGT TCCTGATCTG 
ATGGATGAGT TTATAGCAGA ACGACTTCGA AGTGGTAATG CCTCAACTAT GACACGAAGA 
GGAAGTAGTC CAGGTAGCCT CGAAATTCCC AAAGACCTCC CTGATATTCT AAATAAGCAA 
AACCAAATGC GTCCTATTGA TGACCCAGGT GTACCCTCAG AATGGACTTC TCCTGCCAGT 
GCAGGGAGCA GTGATCTTAT CAGCTCAGAT AGTCATTCGG ATTCTTTCAG CGCTTTCCAG 
TATGATGGCC GAAAATTTGA CAATTTTGGT TTTGGAGCTG ATACTGGGAA TGCATCCTCT 
GCTGACATGG ATTCAGGTTC TGGCCATTAT CAGAGTGCTG AAGAGCAAGA GGTGGCTAGT 
CTAACCACAC TACACATTGA CTCAGAAACA AGCAGTCTTA ATCAACAAGC TTTCTGTACT 
GAAGTTGCAA CGGTTACTGG TTCAGAAAGT GCCTCTCCAG TCCATTCAGC TCTGGGATCC 
AGGTCACAGA CACCTTCTCC TTCCACACTG AATATAGATC ACATGGAACA GAAGGATCTA 
CAGCTCGATG AGAAGCTCCA CCACTCTGTT CTTCAGACAC CAGATGACCT AGAAATCAGT 
GAATTTCCAT CTGAATGCTG TAGTGTGATG GCAGGGGGCA CTCTTACAGG ATGGCATGCT 
GATGTTGCTA CTGTAATGTG GCGAAGGATG CTGGGTATTT TGGGAGATGT CAACGCTATT 
ATGGATCCTG AAATACATGC TCAAGTTTTT GATTACCTCT GTGAACTTTG GCAGAATCTA 
GCTAAGATTA GAGATAATCT TGGCATTTCA ACTGATAACC TGACCTCCCC TTCTCCACCG 
GTTTTAATTC CTCCACTGAG AATTCTTACA CCTTGGCTTT TTAAGGCAAC CATGTTAACT 
GATAAATATA AACAAGGTAA ATTACATGCC TATAAACTTA TTTGTAATAC AATGAAAAGA 
AGACAAGATG TTTCTCCAAA TAGAGATTTT CTAACACATT TCTACAACAT AATGCATTGT 
GGATTGCTTC ACATTGATCA GGATATTGTC AATACAATCA TCAAGCACTG CTCACCTCAA 
TTTTTCTCAC TTGGTTTGCC TGGTGCAACA ATGCTTATTA TGGATTTTAT TGTAGCAGCT 
GGTAGAGTGG CTTCTTCAGC TTTTCTCAAT GCACCAAGAG TGGAAGCACA AGTTCTTCTG 
GGATCTTTGG TTTGCTTTCC CAACTTGTAT TGTGAACTGC CTGCTCTCCA TCCCAACATC 
CCTGATATTG CTGTGTCTCA ATTTACAGAT GTTAAGGAAC TTATAATCAA AACTGTGTTA 
AGCTCTGCAA GAGATGAGCC CTCTGGTCCT GCACGATGTG TAGCACTTTG CAGTTTAGGT 
ATTTGGATTT GTGAAGAACT AGTCCATGAG TCTCATCATC CTCAAATTAA GGAAGCTCTG 
AATGTAATTT GTGTTTCCTT AAAGTTTACT AATAAAACAG TAGCCCATGT AGCTTGTAAC 
ATGCTTCACA TGCTGGTTCA TTATGTACCT AGACTTCAGA TTTACCAGCC TGATTCTCCC 
TTGAAAATTA TTCAAATTCT AATAGCCACT ATTACCCATC TTTTGCCAAG TACGGAAGCG 
TCATCTTATG AAATGGACAA GAGGTTGGTG GTATCCTTAC TTCTGTGTCT TCTGGACTGG 
ATCATGGCCT TACCTCTTAA GACACTGCTC CAACCAGTCC ATGCTACAGG AGCAGAAAAT 
GATAAAATAG AAAAATCTGT TCTTAACTGC ATTTATAAGG TATTACATGG GTGTGTTTAT 
GGAGCTCAGT GTTTTAGCAA TCCGAGGTAT TTTCCAATAA GCCTTTCTGA TTTGGCATCT 
GTAGATTATG ATCCTTTTAT GCATTTGGAA AGCCTAAAAG AGCCTGAACC TTTGCACTCT 
CCTGATTCGG AACGATCTTC TAAACTCCAG CCAGTAACAG AAGTGAAAAC TCAGATGCAG 
CAAGGATTAA TCTCCATAGC TGCCCGCACT GTTATCACAC ATCTGGTGAA TCACTTGGGC 
CATTATCCAA TGAGCGGTGG TCCTGCTATG TTAACAAGTC AGGTGTGTGA GAATCACGAC 
AATCATTACA GTGAAAGCAC TGAACTTTCC CCTGAACTCT TCGAGAGTCC AAATATTCAG 
TTTTTTGTGT TAAACAATAC AACCTTAGTG TCCTGTATCC AGATCAGAGC TGAAGAGAGT 
ATACCTGGAG GAGGTTTATC TGCTGGTCTT GCATCAGCCA ATTCAAATGT CAGAATTATA 
GTACGTGATC TTTCTGGAAA ATATTCATGG GATTCTGCCA TCCTGTATGG ACCACCTCTT 
GTAAGTGGTC TGTCAGAACC TACATCTCTC GTACTTTCAT TGTCTCACCA AGAGAAGCCA 
GAAGAACCTT CTGCATCTAA TGAATGCTTA GAAGATATAA CAGTTAAAGA TGGGATTTCC 
CTCCAGTTTA AAAGAAGTTT TAGAGAAACT GTACCAACTT GGGACACAAT CAGAGATGAA 
GAAGATGTTC TTGATGAGCT TTTGCAGTAT TTAGGTGTAA CCAGTCCTGA ATGCTTACAG 
AGAACTGGAG TCTCACTTAA TATTCCTGCT CCACAGCCTG TGTGCATTTC TGAAAAACAG 
GAGAATGATG TTATTAATGC TATCCTTAAG CAGCATACAG AGGAAAAAGA ATTTGTTGAG 
AAACACTTTA ATGACTTAAA CATGAAAGCT GCAGAACAAG ATGAACCAAC ACCTCAGAAA 
CCTCAGTCAG CATTTTATTA TTGCAGATTG CTTCTTAGTA TATTGGGAAT GAATTCCTGG 
GACAAAAGGA GGAGCTTTCA TCTCCTGAAG AAAAATGAAA AACTACTTAG AGAACTTAGG 
AACTTGGACT CAAGACAGTG CCGAGAGACA CACAAGATTG CAGTATTTTA TGTTGCTGAA 
GGACAAGAAG ACAAACATTC CATTCTCACC AATACAGGAG GAAGTCAAGC ATATGAAGAT 
TTTGTAGCTG GTCTTGGTTG GGAGGTAAAT CTTACAAACC ATTGTGGTTT CATGGGAGGA 
CTACAAAAAA ATAAAAGCAC TGGACTGACC ACACCGTATT TTGCTACCTC TACAGTTGAA 
GTAATATTTC ATGTGTCAAC AAGAATGCCT TCAGATTCTG ATGACTCTTT GACCAAAAAA 
TTGAGACATT TAGGAAATGA TGAAGTGCAC ATTGTTTGGT CAGAGCATAC TAGAGACTAC 
AGGAGAGGAA TTATTCCTAC AGAATTTGGT GATGTCCTTA TTGTTATATA CCCGATGAAA 
AACCACATGT TCAGTATCCA GATAATGAAA AAACCAGAGG TTCCCTTCTT TGGTCCACTC 
TTTGATGGTG CTATTGTGAA TGGAAAGGTC CTACCTATTA TGGTTCGAGC AACAGCTATA 
AATGCAAGCC GTGCTCTGAA ATCCCTGATT CCATTGTATC AAAACTTCTA TGAGGAGAGA 
GCACGATATC TGCAAACGAT TGTCCAGCAC CATTTAGAGC CAACGACATT TGAAGATTTT 
GCAGCACAGG TTTTTTCTCC AGCTCCCTAC CATCATTTAC CATCTGATGC CGTGGGCTCC 
TACCCAGAGA TCCTATCCAG TGAAACTCCC ACAGCGGCGC AGGTAGACGG GGCCGACCTG 
GCCTCTCCAA TGTCTCCTCG AACGAGCAAA AGCCGCATGT CCATGAAGCT ACGTCGCTCC 
TCTGGCTCCG CCAATAAGTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011954209.1
CDS	457..6900
Translation	MFSKKPPGTGKKSTQKVLDTKKDALTRLKHLRIVIGRIFTAEPPEKPREHENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILSSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_012098819.2
Organism	Ovis aries(sheep)
Definition	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012098819.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCC GGGGACAGGG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGGCAG AATCTTTACC 
GCTGAGCCAC CAGAAAAGCC CAGAGAGCAT GAGAATGCAG AATCCATTGA TCTTAAACAG 
TTTTTTGACC AACATTTTTC ACATATATAT TATGTGTTTT TTGAGAATTT TGTTACTATT 
GAAGCTAGTC TTAAACAGAA AGGTCACAAG TCCCAAAGGG AGGAATTGGA TGCTATACTT 
TTTATTTTTG AGAAAATTTT ACAACTTCTT CCAGAAAGAA TTCATCAGCG ATGGCAGTTT 
CATAGTATTG GATTGATTTT AAAGAAACTA CTACACACAG GAAACTCCTT GAAGATTAGG 
CGAGAAGGTG TTCGGCTTTT CTTATTATGG TTGCAAGCTC TTCAGAATAA CTGTAGCAAA 
GAACAGCTTT GGATGTTTTC ATGCTTAATC CCTGGATTTT CAGCACCACA GTCTGAATAT 
GGACCTCGAA CTTTAGATAA CCTCATTAAT CCTCCACTCA ACCTACAAGA AACTCAAGTC 
ACTATAGAGG AAATCACTCC ACTTGTCCCC CCACAATCAG GAGATAAAGG ACAAGAAGAT 
CTTACAAGCT ATTTTCTTGA AGCACTTTTA AAATATATAG TAATTCAGGT AAAAAGTTTA 
GAGTGGAAGA ACAAAGAAAA CCAAGAAAAA GGATTTTCAT TTCTGTTTTC ACATTTTAAG 
AAATATTATT TGCCTTATAT TTTCCCAAAC ATCTGTAAGG AGAATAGTTT ATATCATCCT 
GTACTTGATA TCCCACAGAT GAGACCAAAG CCCCATTATG TCATGATAAA GAAGGATGCT 
GAAACCAATG AGGCAATCTA TTGTACAAAG GAGCCTTTCA TTAAGGCTCG TGTTATTGTC 
ATTCGTTGGC TGGTTTCATT CTGGCTGGAG CCAAAGCCGC ATACAGGACC TCATATTCCT 
GGGATGGAAG GTGAAGTCTT GCCAAAAAAT ATTCAGAGAG CAGCTGCTAG TTTGGTATCC 
AGAGAAGAAA ACAAAAATGA TAATGCTGAT AAAACAGATA GAAGTACAGA ACCTGAACAG 
TCTCATTCCA ACACAAGCAC TCTCACGGAG CGAGAACCTA GCTCTTCTAG TCTCTGTAGT 
ATTGATGAAG AGCATCTCAC AGACATTGAA ATAGTTCGCC GAGTTTTTTC TTCTAAAAGA 
AGTAACGTAA ACTTTGTCAC AGAGATATTT CGTCAGGCAT TTTTATTACC AATTTGCGAA 
GCAGCAGCTA TGAGAAAAGT GGTAAAAGTA TATCAAGAAT GGATCCAGCA AGAGGAAAAA 
CCTTTGTTCA TGCAAGAGCC TGAAGAAATT GTGATCTCTT CTTCAGACCT TCCGTGCATT 
GACAATGTCA CAGACCATGA TATTTCAATG GAAGAAGGAG AAAAAAGAGA AGAGGAAAAT 
GGGACCAATG TTGCTGATCA TGTTCGAAAT TCCACTTGGC CTAAAAATGG AACCTACCAA 
GATATTCTTC ATAATGCTTC TGAAGAAGCC ATAGAACAAA ACATACGAGC TGGTGCCCAG 
GCAGTTTTGC AGGTGTTTAT TATAAATTCT TCAAACATAT TTCTTCTTGA ACCTGCAAAT 
GAGATAAAAA GTCTTCTGGA TGAGCACACA GATATGTGTA AACGAATTCT TAACATTTAT 
CGGTTCATGG TTGTGCAAGT ATCAATGGAC AAAAAGACTT GGGAACAGAT GCTGCTTGTG 
TTGCTCAGAG TCACGGAGTC TGTACTGAAG ATGCCATCAC AAGCTTTTCT ACAGTTCCAG 
GGGAAAAAAA ACATGACTTT GGCAGGTCGA CTTGCAGGAC CACTTTTCCA GACTCTTATA 
GTTGCCTGGA TTAAAGCAAA TCTAAATGTG TACATCTCTC GAGAACTTTG GGATGACTTA 
CTCTCAGTAT TGTCATCGTT GACCTATTGG GAAGAGTTAG CCACTGAATG GTCGCTAACT 
ATGGAGACAC TAACTAAAGT TTTAGCTAGA AATTTGTATA GTTTGGATCT CAGTGATTTA 
CCATTGGATA AGCTGAGTGA ACAGAAACAA AAAAAGCACA AAGGGAAAGG AGTGGGACAT 
GAATTTCAGA AAGTTTCAGT TGACAAGTCA TTTTCTAGAG GATGGAGTCG CGATCAGCCT 
GGCCAAGCCC CAATGAGGCA GAGAAGTGCA ACAACCACTG GTTCCCCAGG CACAGAAAAG 
GCAAGGAGTA TAGTACGGCA AAAAACTGTT GCCATGAGAA GCCGATCCAT TGGTGAATGT 
GCTCTGCCAT CGGCCTATAT ACGCAGTGCT AAAAGTGCTC CTGTTCTGAT CCATACTTCC 
AAACCCTTCT TGCCTGATAT TGTTCTCACT CCCCTTTCTG ATGAGCTTTC AGATATTGAT 
GATGCTCAAA TACTTCCTCG TCCAACTAGA GTCAGACATT TTTCGCAAAG TGAAGACACT 
GGAAATGAAG TTTTTGGTGC TTTGAGTGAG GAGCAGCCAT TGCCTCGAAG TAGCAGCACT 
TCTGACATCT TGGAACCATT CACTGTTGAA CGAGCCAAAG TCAATAAAGA GGACATGAGC 
ACAAAACTGC CTCCTCTTAA TAGTGATATT GGCAGCAGCA ATGCTAATGT TCCTGATCTG 
ATGGATGAGT TTATAGCAGA ACGACTTCGA AGTGGTAATG CCTCAACTAT GACACGAAGA 
GGAAGTAGTC CAGGTAGCCT CGAAATTCCC AAAGACCTCC CTGATATTCT AAATAAGCAA 
AACCAAATGC GTCCTATTGA TGACCCAGGT GTACCCTCAG AATGGACTTC TCCTGCCAGT 
GCAGGGAGCA GTGATCTTAT CAGCTCAGAT AGTCATTCGG ATTCTTTCAG CGCTTTCCAG 
TATGATGGCC GAAAATTTGA CAATTTTGGT TTTGGAGCTG ATACTGGGAA TGCATCCTCT 
GCTGACATGG ATTCAGGTTC TGGCCATTAT CAGAGTGCTG AAGAGCAAGA GGTGGCTAGT 
CTAACCACAC TACACATTGA CTCAGAAACA AGCAGTCTTA ATCAACAAGC TTTCTGTACT 
GAAGTTGCAA CGGTTACTGG TTCAGAAAGT GCCTCTCCAG TCCATTCAGC TCTGGGATCC 
AGGTCACAGA CACCTTCTCC TTCCACACTG AATATAGATC ACATGGAACA GAAGGATCTA 
CAGCTCGATG AGAAGCTCCA CCACTCTGTT CTTCAGACAC CAGATGACCT AGAAATCAGT 
GAATTTCCAT CTGAATGCTG TAGTGTGATG GCAGGGGGCA CTCTTACAGG ATGGCATGCT 
GATGTTGCTA CTGTAATGTG GCGAAGGATG CTGGGTATTT TGGGAGATGT CAACGCTATT 
ATGGATCCTG AAATACATGC TCAAGTTTTT GATTACCTCT GTGAACTTTG GCAGAATCTA 
GCTAAGATTA GAGATAATCT TGGCATTTCA ACTGATAACC TGACCTCCCC TTCTCCACCG 
GTTTTAATTC CTCCACTGAG AATTCTTACA CCTTGGCTTT TTAAGGCAAC CATGTTAACT 
GATAAATATA AACAAGGTAA ATTACATGCC TATAAACTTA TTTGTAATAC AATGAAAAGA 
AGACAAGATG TTTCTCCAAA TAGAGATTTT CTAACACATT TCTACAACAT AATGCATTGT 
GGATTGCTTC ACATTGATCA GGATATTGTC AATACAATCA TCAAGCACTG CTCACCTCAA 
TTTTTCTCAC TTGGTTTGCC TGGTGCAACA ATGCTTATTA TGGATTTTAT TGTAGCAGCT 
GGTAGAGTGG CTTCTTCAGC TTTTCTCAAT GCACCAAGAG TGGAAGCACA AGTTCTTCTG 
GGATCTTTGG TTTGCTTTCC CAACTTGTAT TGTGAACTGC CTGCTCTCCA TCCCAACATC 
CCTGATATTG CTGTGTCTCA ATTTACAGAT GTTAAGGAAC TTATAATCAA AACTGTGTTA 
AGCTCTGCAA GAGATGAGCC CTCTGGTCCT GCACGATGTG TAGCACTTTG CAGTTTAGGT 
ATTTGGATTT GTGAAGAACT AGTCCATGAG TCTCATCATC CTCAAATTAA GGAAGCTCTG 
AATGTAATTT GTGTTTCCTT AAAGTTTACT AATAAAACAG TAGCCCATGT AGCTTGTAAC 
ATGCTTCACA TGCTGGTTCA TTATGTACCT AGACTTCAGA TTTACCAGCC TGATTCTCCC 
TTGAAAATTA TTCAAATTCT AATAGCCACT ATTACCCATC TTTTGCCAAG TACGGAAGCG 
TCATCTTATG AAATGGACAA GAGGTTGGTG GTATCCTTAC TTCTGTGTCT TCTGGACTGG 
ATCATGGCCT TACCTCTTAA GACACTGCTC CAACCAGTCC ATGCTACAGG AGCAGAAAAT 
GATAAAATAG AAAAATCTGT TCTTAACTGC ATTTATAAGG TATTACATGG GTGTGTTTAT 
GGAGCTCAGT GTTTTAGCAA TCCGAGGTAT TTTCCAATAA GCCTTTCTGA TTTGGCATCT 
GTAGATTATG ATCCTTTTAT GCATTTGGAA AGCCTAAAAG AGCCTGAACC TTTGCACTCT 
CCTGATTCGG AACGATCTTC TAAACTCCAG CCAGTAACAG AAGTGAAAAC TCAGATGCAG 
CAAGGATTAA TCTCCATAGC TGCCCGCACT GTTATCACAC ATCTGGTGAA TCACTTGGGC 
CATTATCCAA TGAGCGGTGG TCCTGCTATG TTAACAAGTC AGGTGTGTGA GAATCACGAC 
AATCATTACA GTGAAAGCAC TGAACTTTCC CCTGAACTCT TCGAGAGTCC AAATATTCAG 
TTTTTTGTGT TAAACAATAC AACCTTAGTG TCCTGTATCC AGATCAGAGC TGAAGAGAGT 
ATACCTGGAG GAGGTTTATC TGCTGGTCTT GCATCAGCCA ATTCAAATGT CAGAATTATA 
GTACGTGATC TTTCTGGAAA ATATTCATGG GATTCTGCCA TCCTGTATGG ACCACCTCTT 
GTAAGTGGTC TGTCAGAACC TACATCTCTC GTACTTTCAT TGTCTCACCA AGAGAAGCCA 
GAAGAACCTT CTGCATCTAA TGAATGCTTA GAAGATATAA CAGTTAAAGA TGGGATTTCC 
CTCCAGTTTA AAAGAAGTTT TAGAGAAACT GTACCAACTT GGGACACAAT CAGAGATGAA 
GAAGATGTTC TTGATGAGCT TTTGCAGTAT TTAGGTGTAA CCAGTCCTGA ATGCTTACAG 
AGAACTGGAG TCTCACTTAA TATTCCTGCT CCACAGCCTG TGTGCATTTC TGAAAAACAG 
GAGAATGATG TTATTAATGC TATCCTTAAG CAGCATACAG AGGAAAAAGA ATTTGTTGAG 
AAACACTTTA ATGACTTAAA CATGAAAGCT GCAGAACAAG ATGAACCAAC ACCTCAGAAA 
CCTCAGTCAG CATTTTATTA TTGCAGATTG CTTCTTAGTA TATTGGGAAT GAATTCCTGG 
GACAAAAGGA GGAGCTTTCA TCTCCTGAAG AAAAATGAAA AACTACTTAG AGAACTTAGG 
AACTTGGACT CAAGACAGTG CCGAGAGACA CACAAGATTG CAGTATTTTA TGTTGCTGAA 
GGACAAGAAG ACAAACATTC CATTCTCACC AATACAGGAG GAAGTCAAGC ATATGAAGAT 
TTTGTAGCTG GTCTTGGTTG GGAGGTAAAT CTTACAAACC ATTGTGGTTT CATGGGAGGA 
CTACAAAAAA ATAAAAGCAC TGGACTGACC ACACCGTATT TTGCTACCTC TACAGTTGAA 
GTAATATTTC ATGTGTCAAC AAGAATGCCT TCAGATTCTG ATGACTCTTT GACCAAAAAA 
TTGAGACATT TAGGAAATGA TGAAGTGCAC ATTGTTTGGT CAGAGCATAC TAGAGACTAC 
AGGAGAGGAA TTATTCCTAC AGAATTTGGT GATGTCCTTA TTGTTATATA CCCGATGAAA 
AACCACATGT TCAGTATCCA GATAATGAAA AAACCAGAGG TTCCCTTCTT TGGTCCACTC 
TTTGATGGTG CTATTGTGAA TGGAAAGGTC CTACCTATTA TGGTTCGAGC AACAGCTATA 
AATGCAAGCC GTGCTCTGAA ATCCCTGATT CCATTGTATC AAAACTTCTA TGAGGAGAGA 
GCACGATATC TGCAAACGAT TGTCCAGCAC CATTTAGAGC CAACGACATT TGAAGATTTT 
GCAGCACAGG TTTTTTCTCC AGCTCCCTAC CATCATTTAC CATCTGATGC CGTGGGCTCC 
TACCCAGAGA TCCTATCCAG TGAAACTCCC ACAGCGGCGC AGGTAGACGG GGCCGACCTG 
GCCTCTCCAA TGTCTCCTCG AACGAGCAAA AGCCGCATGT CCATGAAGCT ACGTCGCTCC 
TCTGGCTCCG CCAATAAGTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_012098820.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011954210.1 Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X12, mRNA.

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CloneID	OSm41106
Clone ID Related Accession (Same CDS sequence)	XM_012098820.2
Accession Version	XM_012098820.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	ral GTPase-activating protein subunit alpha-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012098820.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCC GGGGACAGGG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCCCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TAACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGTCAAT 
AAAGAGGACA TGAGCACAAA ACTGCCTCCT CTTAATAGTG ATATTGGCAG CAGCAATGCT 
AATGTTCCTG ATCTGATGGA TGAGTTTATA GCAGAACGAC TTCGAAGTGG TAATGCCTCA 
ACTATGACAC GAAGAGGAAG TAGTCCAGGT AGCCTCGAAA TTCCCAAAGA CCTCCCTGAT 
ATTCTAAATA AGCAAAACCA AATGCGTCCT ATTGATGACC CAGGTGTACC CTCAGAATGG 
ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT CTTATCAGCT CAGATAGTCA TTCGGATTCT 
TTCAGCGCTT TCCAGTATGA TGGCCGAAAA TTTGACAATT TTGGTTTTGG AGCTGATACT 
GGGAATGCAT CCTCTGCTGA CATGGATTCA GGTTCTGGCC ATTATCAGAG TGCTGAAGAG 
CAAGAGGTGG CTAGTCTAAC CACACTACAC ATTGACTCAG AAACAAGCAG TCTTAATCAA 
CAAGCTTTCT GTACTGAAGT TGCAACGGTT ACTGGTTCAG AAAGTGCCTC TCCAGTCCAT 
TCAGCTCTGG GATCCAGGTC ACAGACACCT TCTCCTTCCA CACTGAATAT AGATCACATG 
GAACAGAAGG ATCTACAGCT CGATGAGAAG CTCCACCACT CTGTTCTTCA GACACCAGAT 
GACCTAGAAA TCAGTGAATT TCCATCTGAA TGCTGTAGTG TGATGGCAGG GGGCACTCTT 
ACAGGATGGC ATGCTGATGT TGCTACTGTA ATGTGGCGAA GGATGCTGGG TATTTTGGGA 
GATGTCAACG CTATTATGGA TCCTGAAATA CATGCTCAAG TTTTTGATTA CCTCTGTGAA 
CTTTGGCAGA ATCTAGCTAA GATTAGAGAT AATCTTGGCA TTTCAACTGA TAACCTGACC 
TCCCCTTCTC CACCGGTTTT AATTCCTCCA CTGAGAATTC TTACACCTTG GCTTTTTAAG 
GCAACCATGT TAACTGATAA ATATAAACAA GGTAAATTAC ATGCCTATAA ACTTATTTGT 
AATACAATGA AAAGAAGACA AGATGTTTCT CCAAATAGAG ATTTTCTAAC ACATTTCTAC 
AACATAATGC ATTGTGGATT GCTTCACATT GATCAGGATA TTGTCAATAC AATCATCAAG 
CACTGCTCAC CTCAATTTTT CTCACTTGGT TTGCCTGGTG CAACAATGCT TATTATGGAT 
TTTATTGTAG CAGCTGGTAG AGTGGCTTCT TCAGCTTTTC TCAATGCACC AAGAGTGGAA 
GCACAAGTTC TTCTGGGATC TTTGGTTTGC TTTCCCAACT TGTATTGTGA ACTGCCTGCT 
CTCCATCCCA ACATCCCTGA TATTGCTGTG TCTCAATTTA CAGATGTTAA GGAACTTATA 
ATCAAAACTG TGTTAAGCTC TGCAAGAGAT GAGCCCTCTG GTCCTGCACG ATGTGTAGCA 
CTTTGCAGTT TAGGTATTTG GATTTGTGAA GAACTAGTCC ATGAGTCTCA TCATCCTCAA 
ATTAAGGAAG CTCTGAATGT AATTTGTGTT TCCTTAAAGT TTACTAATAA AACAGTAGCC 
CATGTAGCTT GTAACATGCT TCACATGCTG GTTCATTATG TACCTAGACT TCAGATTTAC 
CAGCCTGATT CTCCCTTGAA AATTATTCAA ATTCTAATAG CCACTATTAC CCATCTTTTG 
CCAAGTACGG AAGCGTCATC TTATGAAATG GACAAGAGGT TGGTGGTATC CTTACTTCTG 
TGTCTTCTGG ACTGGATCAT GGCCTTACCT CTTAAGACAC TGCTCCAACC AGTCCATGCT 
ACAGGAGCAG AAAATGATAA AATAGAAAAA TCTGTTCTTA ACTGCATTTA TAAGGTATTA 
CATGGGTGTG TTTATGGAGC TCAGTGTTTT AGCAATCCGA GGTATTTTCC AATAAGCCTT 
TCTGATTTGG CATCTGTAGA TTATGATCCT TTTATGCATT TGGAAAGCCT AAAAGAGCCT 
GAACCTTTGC ACTCTCCTGA TTCGGAACGA TCTTCTAAAC TCCAGCCAGT AACAGAAGTG 
AAAACTCAGA TGCAGCAAGG ATTAATCTCC ATAGCTGCCC GCACTGTTAT CACACATCTG 
GTGAATCACT TGGGCCATTA TCCAATGAGC GGTGGTCCTG CTATGTTAAC AAGTCAGGTG 
TGTGAGAATC ACGACAATCA TTACAGTGAA AGCACTGAAC TTTCCCCTGA ACTCTTCGAG 
AGTCCAAATA TTCAGTTTTT TGTGTTAAAC AATACAACCT TAGTGTCCTG TATCCAGATC 
AGAGCTGAAG AGAGTATACC TGGAGGAGGT TTATCTGCTG GTCTTGCATC AGCCAATTCA 
AATGTCAGAA TTATAGTACG TGATCTTTCT GGAAAATATT CATGGGATTC TGCCATCCTG 
TATGGACCAC CTCTTGTAAG TGGTCTGTCA GAACCTACAT CTCTCGTACT TTCATTGTCT 
CACCAAGAGA AGCCAGAAGA ACCTTCTGCA TCTAATGAAT GCTTAGAAGA TATAACAGTT 
AAAGATGGGA TTTCCCTCCA GTTTAAAAGA AGTTTTAGAG AAACTGTACC AACTTGGGAC 
ACAATCAGAG ATGAAGAAGA TGTTCTTGAT GAGCTTTTGC AGTATTTAGG TGTAACCAGT 
CCTGAATGCT TACAGAGAAC TGGAGTCTCA CTTAATATTC CTGCTCCACA GCCTGTGTGC 
ATTTCTGAAA AACAGGAGAA TGATGTTATT AATGCTATCC TTAAGCAGCA TACAGAGGAA 
AAAGAATTTG TTGAGAAACA CTTTAATGAC TTAAACATGA AAGCTGCAGA ACAAGATGAA 
CCAACACCTC AGAAACCTCA GTCAGCATTT TATTATTGCA GATTGCTTCT TAGTATATTG 
GGAATGAATT CCTGGGACAA AAGGAGGAGC TTTCATCTCC TGAAGAAAAA TGAAAAACTA 
CTTAGAGAAC TTAGGAACTT GGACTCAAGA CAGTGCCGAG AGACACACAA GATTGCAGTA 
TTTTATGTTG CTGAAGGACA AGAAGACAAA CATTCCATTC TCACCAATAC AGGAGGAAGT 
CAAGCATATG AAGATTTTGT AGCTGGTCTT GGTTGGGAGG TAAATCTTAC AAACCATTGT 
GGTTTCATGG GAGGACTACA AAAAAATAAA AGCACTGGAC TGACCACACC GTATTTTGCT 
ACCTCTACAG TTGAAGTAAT ATTTCATGTG TCAACAAGAA TGCCTTCAGA TTCTGATGAC 
TCTTTGACCA AAAAATTGAG ACATTTAGGA AATGATGAAG TGCACATTGT TTGGTCAGAG 
CATACTAGAG ACTACAGGAG AGGAATTATT CCTACAGAAT TTGGTGATGT CCTTATTGTT 
ATATACCCGA TGAAAAACCA CATGTTCAGT ATCCAGATAA TGAAAAAACC AGAGGTTCCC 
TTCTTTGGTC CACTCTTTGA TGGTGCTATT GTGAATGGAA AGGTCCTACC TATTATGGTT 
CGAGCAACAG CTATAAATGC AAGCCGTGCT CTGAAATCCC TGATTCCATT GTATCAAAAC 
TTCTATGAGG AGAGAGCACG ATATCTGCAA ACGATTGTCC AGCACCATTT AGAGCCAACG 
ACATTTGAAG ATTTTGCAGC ACAGGTTTTT TCTCCAGCTC CCTACCATCA TTTACCATCT 
GATGCCGTGG GCTCCTACCC AGAGATCCTA TCCAGTGAAA CTCCCACAGC GGCGCAGGTA 
GACGGGGCCG ACCTGGCCTC TCCAATGTCT CCTCGAACGA GCAAAAGCCG CATGTCCATG 
AAGCTACGTC GCTCCTCTGG CTCCGCCAAT AAGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011954210.1
CDS	457..6855
Translation	MFSKKPPGTGKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILSSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_012098820.2
Organism	Ovis aries(sheep)
Definition	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012098820.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCC GGGGACAGGG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCCCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TAACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGTCAAT 
AAAGAGGACA TGAGCACAAA ACTGCCTCCT CTTAATAGTG ATATTGGCAG CAGCAATGCT 
AATGTTCCTG ATCTGATGGA TGAGTTTATA GCAGAACGAC TTCGAAGTGG TAATGCCTCA 
ACTATGACAC GAAGAGGAAG TAGTCCAGGT AGCCTCGAAA TTCCCAAAGA CCTCCCTGAT 
ATTCTAAATA AGCAAAACCA AATGCGTCCT ATTGATGACC CAGGTGTACC CTCAGAATGG 
ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT CTTATCAGCT CAGATAGTCA TTCGGATTCT 
TTCAGCGCTT TCCAGTATGA TGGCCGAAAA TTTGACAATT TTGGTTTTGG AGCTGATACT 
GGGAATGCAT CCTCTGCTGA CATGGATTCA GGTTCTGGCC ATTATCAGAG TGCTGAAGAG 
CAAGAGGTGG CTAGTCTAAC CACACTACAC ATTGACTCAG AAACAAGCAG TCTTAATCAA 
CAAGCTTTCT GTACTGAAGT TGCAACGGTT ACTGGTTCAG AAAGTGCCTC TCCAGTCCAT 
TCAGCTCTGG GATCCAGGTC ACAGACACCT TCTCCTTCCA CACTGAATAT AGATCACATG 
GAACAGAAGG ATCTACAGCT CGATGAGAAG CTCCACCACT CTGTTCTTCA GACACCAGAT 
GACCTAGAAA TCAGTGAATT TCCATCTGAA TGCTGTAGTG TGATGGCAGG GGGCACTCTT 
ACAGGATGGC ATGCTGATGT TGCTACTGTA ATGTGGCGAA GGATGCTGGG TATTTTGGGA 
GATGTCAACG CTATTATGGA TCCTGAAATA CATGCTCAAG TTTTTGATTA CCTCTGTGAA 
CTTTGGCAGA ATCTAGCTAA GATTAGAGAT AATCTTGGCA TTTCAACTGA TAACCTGACC 
TCCCCTTCTC CACCGGTTTT AATTCCTCCA CTGAGAATTC TTACACCTTG GCTTTTTAAG 
GCAACCATGT TAACTGATAA ATATAAACAA GGTAAATTAC ATGCCTATAA ACTTATTTGT 
AATACAATGA AAAGAAGACA AGATGTTTCT CCAAATAGAG ATTTTCTAAC ACATTTCTAC 
AACATAATGC ATTGTGGATT GCTTCACATT GATCAGGATA TTGTCAATAC AATCATCAAG 
CACTGCTCAC CTCAATTTTT CTCACTTGGT TTGCCTGGTG CAACAATGCT TATTATGGAT 
TTTATTGTAG CAGCTGGTAG AGTGGCTTCT TCAGCTTTTC TCAATGCACC AAGAGTGGAA 
GCACAAGTTC TTCTGGGATC TTTGGTTTGC TTTCCCAACT TGTATTGTGA ACTGCCTGCT 
CTCCATCCCA ACATCCCTGA TATTGCTGTG TCTCAATTTA CAGATGTTAA GGAACTTATA 
ATCAAAACTG TGTTAAGCTC TGCAAGAGAT GAGCCCTCTG GTCCTGCACG ATGTGTAGCA 
CTTTGCAGTT TAGGTATTTG GATTTGTGAA GAACTAGTCC ATGAGTCTCA TCATCCTCAA 
ATTAAGGAAG CTCTGAATGT AATTTGTGTT TCCTTAAAGT TTACTAATAA AACAGTAGCC 
CATGTAGCTT GTAACATGCT TCACATGCTG GTTCATTATG TACCTAGACT TCAGATTTAC 
CAGCCTGATT CTCCCTTGAA AATTATTCAA ATTCTAATAG CCACTATTAC CCATCTTTTG 
CCAAGTACGG AAGCGTCATC TTATGAAATG GACAAGAGGT TGGTGGTATC CTTACTTCTG 
TGTCTTCTGG ACTGGATCAT GGCCTTACCT CTTAAGACAC TGCTCCAACC AGTCCATGCT 
ACAGGAGCAG AAAATGATAA AATAGAAAAA TCTGTTCTTA ACTGCATTTA TAAGGTATTA 
CATGGGTGTG TTTATGGAGC TCAGTGTTTT AGCAATCCGA GGTATTTTCC AATAAGCCTT 
TCTGATTTGG CATCTGTAGA TTATGATCCT TTTATGCATT TGGAAAGCCT AAAAGAGCCT 
GAACCTTTGC ACTCTCCTGA TTCGGAACGA TCTTCTAAAC TCCAGCCAGT AACAGAAGTG 
AAAACTCAGA TGCAGCAAGG ATTAATCTCC ATAGCTGCCC GCACTGTTAT CACACATCTG 
GTGAATCACT TGGGCCATTA TCCAATGAGC GGTGGTCCTG CTATGTTAAC AAGTCAGGTG 
TGTGAGAATC ACGACAATCA TTACAGTGAA AGCACTGAAC TTTCCCCTGA ACTCTTCGAG 
AGTCCAAATA TTCAGTTTTT TGTGTTAAAC AATACAACCT TAGTGTCCTG TATCCAGATC 
AGAGCTGAAG AGAGTATACC TGGAGGAGGT TTATCTGCTG GTCTTGCATC AGCCAATTCA 
AATGTCAGAA TTATAGTACG TGATCTTTCT GGAAAATATT CATGGGATTC TGCCATCCTG 
TATGGACCAC CTCTTGTAAG TGGTCTGTCA GAACCTACAT CTCTCGTACT TTCATTGTCT 
CACCAAGAGA AGCCAGAAGA ACCTTCTGCA TCTAATGAAT GCTTAGAAGA TATAACAGTT 
AAAGATGGGA TTTCCCTCCA GTTTAAAAGA AGTTTTAGAG AAACTGTACC AACTTGGGAC 
ACAATCAGAG ATGAAGAAGA TGTTCTTGAT GAGCTTTTGC AGTATTTAGG TGTAACCAGT 
CCTGAATGCT TACAGAGAAC TGGAGTCTCA CTTAATATTC CTGCTCCACA GCCTGTGTGC 
ATTTCTGAAA AACAGGAGAA TGATGTTATT AATGCTATCC TTAAGCAGCA TACAGAGGAA 
AAAGAATTTG TTGAGAAACA CTTTAATGAC TTAAACATGA AAGCTGCAGA ACAAGATGAA 
CCAACACCTC AGAAACCTCA GTCAGCATTT TATTATTGCA GATTGCTTCT TAGTATATTG 
GGAATGAATT CCTGGGACAA AAGGAGGAGC TTTCATCTCC TGAAGAAAAA TGAAAAACTA 
CTTAGAGAAC TTAGGAACTT GGACTCAAGA CAGTGCCGAG AGACACACAA GATTGCAGTA 
TTTTATGTTG CTGAAGGACA AGAAGACAAA CATTCCATTC TCACCAATAC AGGAGGAAGT 
CAAGCATATG AAGATTTTGT AGCTGGTCTT GGTTGGGAGG TAAATCTTAC AAACCATTGT 
GGTTTCATGG GAGGACTACA AAAAAATAAA AGCACTGGAC TGACCACACC GTATTTTGCT 
ACCTCTACAG TTGAAGTAAT ATTTCATGTG TCAACAAGAA TGCCTTCAGA TTCTGATGAC 
TCTTTGACCA AAAAATTGAG ACATTTAGGA AATGATGAAG TGCACATTGT TTGGTCAGAG 
CATACTAGAG ACTACAGGAG AGGAATTATT CCTACAGAAT TTGGTGATGT CCTTATTGTT 
ATATACCCGA TGAAAAACCA CATGTTCAGT ATCCAGATAA TGAAAAAACC AGAGGTTCCC 
TTCTTTGGTC CACTCTTTGA TGGTGCTATT GTGAATGGAA AGGTCCTACC TATTATGGTT 
CGAGCAACAG CTATAAATGC AAGCCGTGCT CTGAAATCCC TGATTCCATT GTATCAAAAC 
TTCTATGAGG AGAGAGCACG ATATCTGCAA ACGATTGTCC AGCACCATTT AGAGCCAACG 
ACATTTGAAG ATTTTGCAGC ACAGGTTTTT TCTCCAGCTC CCTACCATCA TTTACCATCT 
GATGCCGTGG GCTCCTACCC AGAGATCCTA TCCAGTGAAA CTCCCACAGC GGCGCAGGTA 
GACGGGGCCG ACCTGGCCTC TCCAATGTCT CCTCGAACGA GCAAAAGCCG CATGTCCATG 
AAGCTACGTC GCTCCTCTGG CTCCGCCAAT AAGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_012098821.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011954211.1 Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X14, mRNA.

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CloneID	OSm41107
Clone ID Related Accession (Same CDS sequence)	XM_012098821.2
Accession Version	XM_012098821.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	ral GTPase-activating protein subunit alpha-1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012098821.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCC GGGGACAGGG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCCCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TAACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAGCCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGTGGG CTCCTACCCA 
GAGATCCTAT CCAGTGAAAC TCCCACAGCG GCGCAGGTAG ACGGGGCCGA CCTGGCCTCT 
CCAATGTCTC CTCGAACGAG CAAAAGCCGC ATGTCCATGA AGCTACGTCG CTCCTCTGGC 
TCCGCCAATA AGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011954211.1
CDS	457..6714
Translation	MFSKKPPGTGKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTDHDISMEEGEKREEENGTNVADHVRNSTWPKNGTYQDILHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILSSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_012098821.2
Organism	Ovis aries(sheep)
Definition	Ovis aries Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012098821.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCC GGGGACAGGG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCCCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGAGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTA 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAAAGGATT TTCATTTCTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCCCA TTATGTCATG 
ATAAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCGT GCATTGACAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGGGAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGAACCT ACCAAGATAT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ATTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAGTCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTACAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGAGAA 
CTTTGGGATG ACTTACTCTC AGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TAACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT GTATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG 
AAAGGAGTGG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCTCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAATAA GCAAAACCAA 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAGTATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCTGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TTGACTCAGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGAAGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTGAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ACATAATGCA TTGTGGATTG 
CTTCACATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC AACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACGGA AGCGTCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTTCTTAA CTGCATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAGAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATC ACACATCTGG TGAATCACTT GGGCCATTAT 
CCAATGAGCG GTGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCTGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTGTCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATCAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGAACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTTAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACACCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA 
AGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGACT GACCACACCG TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCATGTGT CAACAAGAAT GCCTTCAGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTTA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCCCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAGCCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGTGGG CTCCTACCCA 
GAGATCCTAT CCAGTGAAAC TCCCACAGCG GCGCAGGTAG ACGGGGCCGA CCTGGCCTCT 
CCAATGTCTC CTCGAACGAG CAAAAGCCGC ATGTCCATGA AGCTACGTCG CTCCTCTGGC 
TCCGCCAATA AGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	RALGAPA1
Entrez Gene ID	101120113
Full Name	Ral GTPase activating protein catalytic alpha subunit 1
Gene Type	protein-coding
Organism	Ovis aries(sheep)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

RALGAPA1 cDNA ORF clone, Ovis aries(sheep)

Gene

Clones

gRNAs

Target ORF information:

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