DICER1 gene cDNA ORF clone, Ovis aries(sheep)

mRNA	Protein	Name
XM_012112869.2	XP_011968259.1	endoribonuclease Dicer isoform X1
XM_012112871.2	XP_011968261.1	endoribonuclease Dicer isoform X1
XM_004017979.3	XP_004018028.1	endoribonuclease Dicer isoform X1
XM_012112870.2	XP_011968260.1	endoribonuclease Dicer isoform X1
XM_012112873.2	XP_011968263.1	endoribonuclease Dicer isoform X2
XM_027957365.1	XP_027813166.1	endoribonuclease Dicer
XM_015088705.1	XP_014944191.1	endoribonuclease Dicer isoform X2
XM_015102037.1	XP_014957523.1	endoribonuclease Dicer isoform X1
XM_027957367.1	XP_027813168.1	endoribonuclease Dicer
XM_012099009.2	XP_011954399.1	endoribonuclease Dicer isoform X1
XM_015102036.1	XP_014957522.1	endoribonuclease Dicer isoform X1
XM_015088696.1	XP_014944182.1	endoribonuclease Dicer isoform X1
XM_015088702.1	XP_014944188.1	endoribonuclease Dicer isoform X1
XM_012112872.2	XP_011968262.1	endoribonuclease Dicer isoform X1
XM_015088703.1	XP_014944189.1	endoribonuclease Dicer isoform X2
XM_027957364.1	XP_027813165.1	endoribonuclease Dicer

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOv02800	XM_012112871.2 Latest version!	Ovis aries musimon dicer 1, ribonuclease type III (DICER1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv02800	XM_012112872.2 Latest version!	Ovis aries musimon dicer 1, ribonuclease type III (DICER1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv02800	XM_012112870.2 Latest version!	Ovis aries musimon dicer 1, ribonuclease type III (DICER1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv02800	XM_015088702.1 Latest version!	Ovis aries musimon dicer 1, ribonuclease type III (DICER1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv02800	XM_012112869.2 Latest version!	Ovis aries musimon dicer 1, ribonuclease type III (DICER1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv02800	XM_015088696.1 Latest version!	Ovis aries musimon dicer 1, ribonuclease type III (DICER1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv02801	XM_012112873.2 Latest version!	Ovis aries musimon dicer 1, ribonuclease type III (DICER1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv02801	XM_015088703.1 Latest version!	Ovis aries musimon dicer 1, ribonuclease type III (DICER1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv02801	XM_015088705.1 Latest version!	Ovis aries musimon dicer 1, ribonuclease type III (DICER1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm14330	XM_015102036.1 Latest version!	Ovis aries dicer 1, ribonuclease type III (DICER1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm14330	XM_004017979.3 Latest version!	Ovis aries dicer 1, ribonuclease type III (DICER1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm14330	XM_012099009.2 Latest version!	Ovis aries dicer 1, ribonuclease type III (DICER1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm14330	XM_015102037.1 Latest version!	Ovis aries dicer 1, ribonuclease type III (DICER1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm68060	XM_027957367.1 Latest version!	Ovis aries dicer 1, ribonuclease III (DICER1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm68060	XM_027957364.1 Latest version!	Ovis aries dicer 1, ribonuclease III (DICER1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm68060	XM_027957365.1 Latest version!	Ovis aries dicer 1, ribonuclease III (DICER1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

DICER1 ( XM_012112869.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011968259.1 Ovis aries musimon dicer 1, ribonuclease type III (DICER1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OOv02800
Clone ID Related Accession (Same CDS sequence)	XM_012112869.2 , XM_012112871.2 , XM_012112870.2 , XM_015088696.1 , XM_015088702.1 , XM_012112872.2
Accession Version	XM_012112869.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942251.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012112869.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTGCTGCT CACTAAAGAG 
CTGTCTTACC AGATCAGGGG AGACTTCAAC AGAAGTGGCA GGAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAACT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC GTCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTT AGGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCGTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTA GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTACAG AAACACATCA AACACGAGCA AGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTAAGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CACCTGCCTC CCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAGTA CAAGCCGTAC GAGCGGCAGC AGTTTGAAAG CGTGGAATGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGTG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC ATTTACCAAT 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CGGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT TGATCTGCCC 
ACAGAGTATC GATCCTATGT TCAGTCTAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGATACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCAG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGACGA TGACGATGTG TTCCCACCAT ACGTGCTGAG GCCTGAGGAC 
GGTCCTCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG ATTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAGA 
CTGCACAAAA TTGGTGAACT GGATGACCAT TTGATGCCCG TTGGGAAGGA GACTGTTAAA 
TATGAGGAGG AGCTGGATCT GCACGATGAA GAAGAGACTA GTGTTCCAGG AAGACCGGGC 
TCCACGAAGC GAAGGCAGTG CTACCCGAAA GCAATCCCCG AATGTTTGAG GGAGAGCTAC 
CCCAAGCCCG GTCAGCCCTG TTACCTGTAC GTGATCGGCA TGGTCCTGAC GACGCCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CCGAGGACAC CACGAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATTACAAG ACTTCATCAG TATATATTCT CGCATATTCT TCGGCTTGAA 
AAACCTGCCC TAGAATTTAA ACCCGCAGAC GCGGACTCAG CCTACTGTGT TCTACCTCTT 
AACGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGCATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCCTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTTAA TCTTTTGACA 
CCTCGCCATT TGAATCAGAA GGGGAAAGCT CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AAAGCCTGCA GAATAAACAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTCTGTCTCC CCAGCATACT CTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACAG CCAGCGATGC TGGTGTGGGA 
GTCAGATCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAGTTGCT AACTCCTCTT CAGCAGAAAA TGAGAATTAC 
TGTAAGCATA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGCTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAGTTGA AGTTGCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCCGCC 
GTGCCTGGTA CTACAGAGGC TGGGGAGGCG CCCCCGGACA GGACGGCTTC TGAGCAGAGC 
CCTTCTCCTG GGCACTCCTC GAGGACGCTC GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
CTGACCCTTT CGAATGCCAG CGACGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTGA AGCACGCCAT CACCACGTAT CTCTTCTGCA CGTACCCCGA CGCGCACGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAGCTGG ACGATGACTT TGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCCCAAGG AGGACGCCGA CGATGAGGAC 
GACTTCCTGG AGTACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT CCTTTTTCCG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTGGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCGTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC TGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGCGT CGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA GAGCATAGCT 
GACTGCGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGCG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTATGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ATGCAGACAG GACGCTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAACACCA TCTTCGCGTC GTTGGCTGTG AAATATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA CGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC CGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTTGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011968259.1
CDS	505..6273
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRSGRRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVTASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILRSNAETATDLVVVDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCERLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPADADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISVANSSSAENENYCKHSTIVVPENAAHQGANRTSSLENHDQMSVNCRTLFSESPGKLQVEVATDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTEAGEAPPDRTASEQSPSPGHSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAPKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRMAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_012112869.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon dicer 1, ribonuclease type III (DICER1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012112869.2

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTGCTGCT CACTAAAGAG 
CTGTCTTACC AGATCAGGGG AGACTTCAAC AGAAGTGGCA GGAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAACT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC GTCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTT AGGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCGTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTA GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTACAG AAACACATCA AACACGAGCA AGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTAAGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CACCTGCCTC CCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAGTA CAAGCCGTAC GAGCGGCAGC AGTTTGAAAG CGTGGAATGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGTG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC ATTTACCAAT 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CGGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT TGATCTGCCC 
ACAGAGTATC GATCCTATGT TCAGTCTAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGATACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCAG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGACGA TGACGATGTG TTCCCACCAT ACGTGCTGAG GCCTGAGGAC 
GGTCCTCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG ATTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAGA 
CTGCACAAAA TTGGTGAACT GGATGACCAT TTGATGCCCG TTGGGAAGGA GACTGTTAAA 
TATGAGGAGG AGCTGGATCT GCACGATGAA GAAGAGACTA GTGTTCCAGG AAGACCGGGC 
TCCACGAAGC GAAGGCAGTG CTACCCGAAA GCAATCCCCG AATGTTTGAG GGAGAGCTAC 
CCCAAGCCCG GTCAGCCCTG TTACCTGTAC GTGATCGGCA TGGTCCTGAC GACGCCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CCGAGGACAC CACGAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATTACAAG ACTTCATCAG TATATATTCT CGCATATTCT TCGGCTTGAA 
AAACCTGCCC TAGAATTTAA ACCCGCAGAC GCGGACTCAG CCTACTGTGT TCTACCTCTT 
AACGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGCATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCCTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTTAA TCTTTTGACA 
CCTCGCCATT TGAATCAGAA GGGGAAAGCT CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AAAGCCTGCA GAATAAACAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTCTGTCTCC CCAGCATACT CTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACAG CCAGCGATGC TGGTGTGGGA 
GTCAGATCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAGTTGCT AACTCCTCTT CAGCAGAAAA TGAGAATTAC 
TGTAAGCATA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGCTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAGTTGA AGTTGCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCCGCC 
GTGCCTGGTA CTACAGAGGC TGGGGAGGCG CCCCCGGACA GGACGGCTTC TGAGCAGAGC 
CCTTCTCCTG GGCACTCCTC GAGGACGCTC GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
CTGACCCTTT CGAATGCCAG CGACGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTGA AGCACGCCAT CACCACGTAT CTCTTCTGCA CGTACCCCGA CGCGCACGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAGCTGG ACGATGACTT TGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCCCAAGG AGGACGCCGA CGATGAGGAC 
GACTTCCTGG AGTACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT CCTTTTTCCG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTGGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCGTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC TGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGCGT CGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA GAGCATAGCT 
GACTGCGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGCG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTATGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ATGCAGACAG GACGCTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAACACCA TCTTCGCGTC GTTGGCTGTG AAATATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA CGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC CGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTTGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_012112871.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011968261.1 Ovis aries musimon dicer 1, ribonuclease type III (DICER1), transcript variant X8, mRNA.

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CloneID	OOv02800
Clone ID Related Accession (Same CDS sequence)	XM_012112869.2 , XM_012112871.2 , XM_012112870.2 , XM_015088696.1 , XM_015088702.1 , XM_012112872.2
Accession Version	XM_012112871.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942251.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012112871.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTGCTGCT CACTAAAGAG 
CTGTCTTACC AGATCAGGGG AGACTTCAAC AGAAGTGGCA GGAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAACT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC GTCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTT AGGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCGTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTA GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTACAG AAACACATCA AACACGAGCA AGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTAAGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CACCTGCCTC CCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAGTA CAAGCCGTAC GAGCGGCAGC AGTTTGAAAG CGTGGAATGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGTG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC ATTTACCAAT 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CGGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT TGATCTGCCC 
ACAGAGTATC GATCCTATGT TCAGTCTAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGATACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCAG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGACGA TGACGATGTG TTCCCACCAT ACGTGCTGAG GCCTGAGGAC 
GGTCCTCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG ATTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAGA 
CTGCACAAAA TTGGTGAACT GGATGACCAT TTGATGCCCG TTGGGAAGGA GACTGTTAAA 
TATGAGGAGG AGCTGGATCT GCACGATGAA GAAGAGACTA GTGTTCCAGG AAGACCGGGC 
TCCACGAAGC GAAGGCAGTG CTACCCGAAA GCAATCCCCG AATGTTTGAG GGAGAGCTAC 
CCCAAGCCCG GTCAGCCCTG TTACCTGTAC GTGATCGGCA TGGTCCTGAC GACGCCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CCGAGGACAC CACGAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATTACAAG ACTTCATCAG TATATATTCT CGCATATTCT TCGGCTTGAA 
AAACCTGCCC TAGAATTTAA ACCCGCAGAC GCGGACTCAG CCTACTGTGT TCTACCTCTT 
AACGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGCATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCCTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTTAA TCTTTTGACA 
CCTCGCCATT TGAATCAGAA GGGGAAAGCT CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AAAGCCTGCA GAATAAACAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTCTGTCTCC CCAGCATACT CTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACAG CCAGCGATGC TGGTGTGGGA 
GTCAGATCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAGTTGCT AACTCCTCTT CAGCAGAAAA TGAGAATTAC 
TGTAAGCATA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGCTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAGTTGA AGTTGCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCCGCC 
GTGCCTGGTA CTACAGAGGC TGGGGAGGCG CCCCCGGACA GGACGGCTTC TGAGCAGAGC 
CCTTCTCCTG GGCACTCCTC GAGGACGCTC GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
CTGACCCTTT CGAATGCCAG CGACGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTGA AGCACGCCAT CACCACGTAT CTCTTCTGCA CGTACCCCGA CGCGCACGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAGCTGG ACGATGACTT TGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCCCAAGG AGGACGCCGA CGATGAGGAC 
GACTTCCTGG AGTACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT CCTTTTTCCG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTGGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCGTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC TGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGCGT CGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA GAGCATAGCT 
GACTGCGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGCG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTATGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ATGCAGACAG GACGCTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAACACCA TCTTCGCGTC GTTGGCTGTG AAATATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA CGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC CGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTTGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011968261.1
CDS	533..6301
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRSGRRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVTASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILRSNAETATDLVVVDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCERLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPADADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISVANSSSAENENYCKHSTIVVPENAAHQGANRTSSLENHDQMSVNCRTLFSESPGKLQVEVATDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTEAGEAPPDRTASEQSPSPGHSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAPKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRMAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_012112871.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon dicer 1, ribonuclease type III (DICER1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012112871.2

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTGCTGCT CACTAAAGAG 
CTGTCTTACC AGATCAGGGG AGACTTCAAC AGAAGTGGCA GGAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAACT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC GTCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTT AGGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCGTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTA GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTACAG AAACACATCA AACACGAGCA AGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTAAGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CACCTGCCTC CCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAGTA CAAGCCGTAC GAGCGGCAGC AGTTTGAAAG CGTGGAATGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGTG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC ATTTACCAAT 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CGGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT TGATCTGCCC 
ACAGAGTATC GATCCTATGT TCAGTCTAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGATACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCAG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGACGA TGACGATGTG TTCCCACCAT ACGTGCTGAG GCCTGAGGAC 
GGTCCTCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG ATTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAGA 
CTGCACAAAA TTGGTGAACT GGATGACCAT TTGATGCCCG TTGGGAAGGA GACTGTTAAA 
TATGAGGAGG AGCTGGATCT GCACGATGAA GAAGAGACTA GTGTTCCAGG AAGACCGGGC 
TCCACGAAGC GAAGGCAGTG CTACCCGAAA GCAATCCCCG AATGTTTGAG GGAGAGCTAC 
CCCAAGCCCG GTCAGCCCTG TTACCTGTAC GTGATCGGCA TGGTCCTGAC GACGCCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CCGAGGACAC CACGAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATTACAAG ACTTCATCAG TATATATTCT CGCATATTCT TCGGCTTGAA 
AAACCTGCCC TAGAATTTAA ACCCGCAGAC GCGGACTCAG CCTACTGTGT TCTACCTCTT 
AACGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGCATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCCTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTTAA TCTTTTGACA 
CCTCGCCATT TGAATCAGAA GGGGAAAGCT CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AAAGCCTGCA GAATAAACAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTCTGTCTCC CCAGCATACT CTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACAG CCAGCGATGC TGGTGTGGGA 
GTCAGATCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAGTTGCT AACTCCTCTT CAGCAGAAAA TGAGAATTAC 
TGTAAGCATA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGCTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAGTTGA AGTTGCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCCGCC 
GTGCCTGGTA CTACAGAGGC TGGGGAGGCG CCCCCGGACA GGACGGCTTC TGAGCAGAGC 
CCTTCTCCTG GGCACTCCTC GAGGACGCTC GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
CTGACCCTTT CGAATGCCAG CGACGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTGA AGCACGCCAT CACCACGTAT CTCTTCTGCA CGTACCCCGA CGCGCACGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAGCTGG ACGATGACTT TGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCCCAAGG AGGACGCCGA CGATGAGGAC 
GACTTCCTGG AGTACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT CCTTTTTCCG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTGGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCGTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC TGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGCGT CGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA GAGCATAGCT 
GACTGCGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGCG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTATGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ATGCAGACAG GACGCTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAACACCA TCTTCGCGTC GTTGGCTGTG AAATATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA CGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC CGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTTGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_012112870.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011968260.1 Ovis aries musimon dicer 1, ribonuclease type III (DICER1), transcript variant X6, mRNA.

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CloneID	OOv02800
Clone ID Related Accession (Same CDS sequence)	XM_012112869.2 , XM_012112871.2 , XM_012112870.2 , XM_015088696.1 , XM_015088702.1 , XM_012112872.2
Accession Version	XM_012112870.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942251.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012112870.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTGCTGCT CACTAAAGAG 
CTGTCTTACC AGATCAGGGG AGACTTCAAC AGAAGTGGCA GGAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAACT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC GTCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTT AGGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCGTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTA GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTACAG AAACACATCA AACACGAGCA AGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTAAGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CACCTGCCTC CCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAGTA CAAGCCGTAC GAGCGGCAGC AGTTTGAAAG CGTGGAATGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGTG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC ATTTACCAAT 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CGGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT TGATCTGCCC 
ACAGAGTATC GATCCTATGT TCAGTCTAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGATACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCAG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGACGA TGACGATGTG TTCCCACCAT ACGTGCTGAG GCCTGAGGAC 
GGTCCTCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG ATTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAGA 
CTGCACAAAA TTGGTGAACT GGATGACCAT TTGATGCCCG TTGGGAAGGA GACTGTTAAA 
TATGAGGAGG AGCTGGATCT GCACGATGAA GAAGAGACTA GTGTTCCAGG AAGACCGGGC 
TCCACGAAGC GAAGGCAGTG CTACCCGAAA GCAATCCCCG AATGTTTGAG GGAGAGCTAC 
CCCAAGCCCG GTCAGCCCTG TTACCTGTAC GTGATCGGCA TGGTCCTGAC GACGCCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CCGAGGACAC CACGAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATTACAAG ACTTCATCAG TATATATTCT CGCATATTCT TCGGCTTGAA 
AAACCTGCCC TAGAATTTAA ACCCGCAGAC GCGGACTCAG CCTACTGTGT TCTACCTCTT 
AACGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGCATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCCTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTTAA TCTTTTGACA 
CCTCGCCATT TGAATCAGAA GGGGAAAGCT CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AAAGCCTGCA GAATAAACAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTCTGTCTCC CCAGCATACT CTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACAG CCAGCGATGC TGGTGTGGGA 
GTCAGATCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAGTTGCT AACTCCTCTT CAGCAGAAAA TGAGAATTAC 
TGTAAGCATA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGCTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAGTTGA AGTTGCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCCGCC 
GTGCCTGGTA CTACAGAGGC TGGGGAGGCG CCCCCGGACA GGACGGCTTC TGAGCAGAGC 
CCTTCTCCTG GGCACTCCTC GAGGACGCTC GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
CTGACCCTTT CGAATGCCAG CGACGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTGA AGCACGCCAT CACCACGTAT CTCTTCTGCA CGTACCCCGA CGCGCACGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAGCTGG ACGATGACTT TGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCCCAAGG AGGACGCCGA CGATGAGGAC 
GACTTCCTGG AGTACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT CCTTTTTCCG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTGGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCGTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC TGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGCGT CGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA GAGCATAGCT 
GACTGCGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGCG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTATGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ATGCAGACAG GACGCTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAACACCA TCTTCGCGTC GTTGGCTGTG AAATATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA CGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC CGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTTGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011968260.1
CDS	3837..9605
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRSGRRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVTASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILRSNAETATDLVVVDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCERLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPADADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISVANSSSAENENYCKHSTIVVPENAAHQGANRTSSLENHDQMSVNCRTLFSESPGKLQVEVATDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTEAGEAPPDRTASEQSPSPGHSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAPKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRMAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_012112870.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon dicer 1, ribonuclease type III (DICER1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012112870.2

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTGCTGCT CACTAAAGAG 
CTGTCTTACC AGATCAGGGG AGACTTCAAC AGAAGTGGCA GGAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAACT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC GTCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTT AGGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCGTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTA GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTACAG AAACACATCA AACACGAGCA AGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTAAGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CACCTGCCTC CCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAGTA CAAGCCGTAC GAGCGGCAGC AGTTTGAAAG CGTGGAATGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGTG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC ATTTACCAAT 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CGGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT TGATCTGCCC 
ACAGAGTATC GATCCTATGT TCAGTCTAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGATACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCAG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGACGA TGACGATGTG TTCCCACCAT ACGTGCTGAG GCCTGAGGAC 
GGTCCTCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG ATTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAGA 
CTGCACAAAA TTGGTGAACT GGATGACCAT TTGATGCCCG TTGGGAAGGA GACTGTTAAA 
TATGAGGAGG AGCTGGATCT GCACGATGAA GAAGAGACTA GTGTTCCAGG AAGACCGGGC 
TCCACGAAGC GAAGGCAGTG CTACCCGAAA GCAATCCCCG AATGTTTGAG GGAGAGCTAC 
CCCAAGCCCG GTCAGCCCTG TTACCTGTAC GTGATCGGCA TGGTCCTGAC GACGCCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CCGAGGACAC CACGAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATTACAAG ACTTCATCAG TATATATTCT CGCATATTCT TCGGCTTGAA 
AAACCTGCCC TAGAATTTAA ACCCGCAGAC GCGGACTCAG CCTACTGTGT TCTACCTCTT 
AACGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGCATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCCTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTTAA TCTTTTGACA 
CCTCGCCATT TGAATCAGAA GGGGAAAGCT CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AAAGCCTGCA GAATAAACAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTCTGTCTCC CCAGCATACT CTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACAG CCAGCGATGC TGGTGTGGGA 
GTCAGATCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAGTTGCT AACTCCTCTT CAGCAGAAAA TGAGAATTAC 
TGTAAGCATA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGCTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAGTTGA AGTTGCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCCGCC 
GTGCCTGGTA CTACAGAGGC TGGGGAGGCG CCCCCGGACA GGACGGCTTC TGAGCAGAGC 
CCTTCTCCTG GGCACTCCTC GAGGACGCTC GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
CTGACCCTTT CGAATGCCAG CGACGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTGA AGCACGCCAT CACCACGTAT CTCTTCTGCA CGTACCCCGA CGCGCACGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAGCTGG ACGATGACTT TGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCCCAAGG AGGACGCCGA CGATGAGGAC 
GACTTCCTGG AGTACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT CCTTTTTCCG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTGGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCGTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC TGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGCGT CGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA GAGCATAGCT 
GACTGCGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGCG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTATGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ATGCAGACAG GACGCTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAACACCA TCTTCGCGTC GTTGGCTGTG AAATATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA CGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC CGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTTGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_015088696.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014944182.1 Ovis aries musimon dicer 1, ribonuclease type III (DICER1), transcript variant X1, mRNA.

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CloneID	OOv02800
Clone ID Related Accession (Same CDS sequence)	XM_012112869.2 , XM_012112871.2 , XM_012112870.2 , XM_015088696.1 , XM_015088702.1 , XM_012112872.2
Accession Version	XM_015088696.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942251.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTGCTGCT CACTAAAGAG 
CTGTCTTACC AGATCAGGGG AGACTTCAAC AGAAGTGGCA GGAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAACT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC GTCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTT AGGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCGTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTA GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTACAG AAACACATCA AACACGAGCA AGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTAAGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CACCTGCCTC CCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAGTA CAAGCCGTAC GAGCGGCAGC AGTTTGAAAG CGTGGAATGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGTG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC ATTTACCAAT 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CGGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT TGATCTGCCC 
ACAGAGTATC GATCCTATGT TCAGTCTAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGATACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCAG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGACGA TGACGATGTG TTCCCACCAT ACGTGCTGAG GCCTGAGGAC 
GGTCCTCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG ATTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAGA 
CTGCACAAAA TTGGTGAACT GGATGACCAT TTGATGCCCG TTGGGAAGGA GACTGTTAAA 
TATGAGGAGG AGCTGGATCT GCACGATGAA GAAGAGACTA GTGTTCCAGG AAGACCGGGC 
TCCACGAAGC GAAGGCAGTG CTACCCGAAA GCAATCCCCG AATGTTTGAG GGAGAGCTAC 
CCCAAGCCCG GTCAGCCCTG TTACCTGTAC GTGATCGGCA TGGTCCTGAC GACGCCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CCGAGGACAC CACGAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATTACAAG ACTTCATCAG TATATATTCT CGCATATTCT TCGGCTTGAA 
AAACCTGCCC TAGAATTTAA ACCCGCAGAC GCGGACTCAG CCTACTGTGT TCTACCTCTT 
AACGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGCATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCCTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTTAA TCTTTTGACA 
CCTCGCCATT TGAATCAGAA GGGGAAAGCT CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AAAGCCTGCA GAATAAACAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTCTGTCTCC CCAGCATACT CTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACAG CCAGCGATGC TGGTGTGGGA 
GTCAGATCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAGTTGCT AACTCCTCTT CAGCAGAAAA TGAGAATTAC 
TGTAAGCATA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGCTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAGTTGA AGTTGCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCCGCC 
GTGCCTGGTA CTACAGAGGC TGGGGAGGCG CCCCCGGACA GGACGGCTTC TGAGCAGAGC 
CCTTCTCCTG GGCACTCCTC GAGGACGCTC GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
CTGACCCTTT CGAATGCCAG CGACGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTGA AGCACGCCAT CACCACGTAT CTCTTCTGCA CGTACCCCGA CGCGCACGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAGCTGG ACGATGACTT TGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCCCAAGG AGGACGCCGA CGATGAGGAC 
GACTTCCTGG AGTACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT CCTTTTTCCG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTGGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCGTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC TGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGCGT CGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA GAGCATAGCT 
GACTGCGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGCG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTATGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ATGCAGACAG GACGCTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAACACCA TCTTCGCGTC GTTGGCTGTG AAATATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA CGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC CGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTTGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014944182.1
CDS	2770..8538
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRSGRRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVTASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILRSNAETATDLVVVDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCERLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPADADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISVANSSSAENENYCKHSTIVVPENAAHQGANRTSSLENHDQMSVNCRTLFSESPGKLQVEVATDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTEAGEAPPDRTASEQSPSPGHSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAPKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRMAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_015088696.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon dicer 1, ribonuclease type III (DICER1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015088696.1

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTGCTGCT CACTAAAGAG 
CTGTCTTACC AGATCAGGGG AGACTTCAAC AGAAGTGGCA GGAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAACT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC GTCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTT AGGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCGTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTA GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTACAG AAACACATCA AACACGAGCA AGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTAAGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CACCTGCCTC CCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAGTA CAAGCCGTAC GAGCGGCAGC AGTTTGAAAG CGTGGAATGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGTG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC ATTTACCAAT 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CGGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT TGATCTGCCC 
ACAGAGTATC GATCCTATGT TCAGTCTAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGATACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCAG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGACGA TGACGATGTG TTCCCACCAT ACGTGCTGAG GCCTGAGGAC 
GGTCCTCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG ATTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAGA 
CTGCACAAAA TTGGTGAACT GGATGACCAT TTGATGCCCG TTGGGAAGGA GACTGTTAAA 
TATGAGGAGG AGCTGGATCT GCACGATGAA GAAGAGACTA GTGTTCCAGG AAGACCGGGC 
TCCACGAAGC GAAGGCAGTG CTACCCGAAA GCAATCCCCG AATGTTTGAG GGAGAGCTAC 
CCCAAGCCCG GTCAGCCCTG TTACCTGTAC GTGATCGGCA TGGTCCTGAC GACGCCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CCGAGGACAC CACGAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATTACAAG ACTTCATCAG TATATATTCT CGCATATTCT TCGGCTTGAA 
AAACCTGCCC TAGAATTTAA ACCCGCAGAC GCGGACTCAG CCTACTGTGT TCTACCTCTT 
AACGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGCATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCCTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTTAA TCTTTTGACA 
CCTCGCCATT TGAATCAGAA GGGGAAAGCT CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AAAGCCTGCA GAATAAACAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTCTGTCTCC CCAGCATACT CTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACAG CCAGCGATGC TGGTGTGGGA 
GTCAGATCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAGTTGCT AACTCCTCTT CAGCAGAAAA TGAGAATTAC 
TGTAAGCATA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGCTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAGTTGA AGTTGCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCCGCC 
GTGCCTGGTA CTACAGAGGC TGGGGAGGCG CCCCCGGACA GGACGGCTTC TGAGCAGAGC 
CCTTCTCCTG GGCACTCCTC GAGGACGCTC GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
CTGACCCTTT CGAATGCCAG CGACGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTGA AGCACGCCAT CACCACGTAT CTCTTCTGCA CGTACCCCGA CGCGCACGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAGCTGG ACGATGACTT TGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCCCAAGG AGGACGCCGA CGATGAGGAC 
GACTTCCTGG AGTACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT CCTTTTTCCG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTGGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCGTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC TGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGCGT CGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA GAGCATAGCT 
GACTGCGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGCG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTATGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ATGCAGACAG GACGCTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAACACCA TCTTCGCGTC GTTGGCTGTG AAATATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA CGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC CGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTTGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_015088702.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014944188.1 Ovis aries musimon dicer 1, ribonuclease type III (DICER1), transcript variant X7, mRNA.

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CloneID	OOv02800
Clone ID Related Accession (Same CDS sequence)	XM_012112869.2 , XM_012112871.2 , XM_012112870.2 , XM_015088696.1 , XM_015088702.1 , XM_012112872.2
Accession Version	XM_015088702.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942251.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTGCTGCT CACTAAAGAG 
CTGTCTTACC AGATCAGGGG AGACTTCAAC AGAAGTGGCA GGAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAACT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC GTCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTT AGGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCGTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTA GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTACAG AAACACATCA AACACGAGCA AGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTAAGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CACCTGCCTC CCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAGTA CAAGCCGTAC GAGCGGCAGC AGTTTGAAAG CGTGGAATGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGTG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC ATTTACCAAT 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CGGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT TGATCTGCCC 
ACAGAGTATC GATCCTATGT TCAGTCTAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGATACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCAG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGACGA TGACGATGTG TTCCCACCAT ACGTGCTGAG GCCTGAGGAC 
GGTCCTCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG ATTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAGA 
CTGCACAAAA TTGGTGAACT GGATGACCAT TTGATGCCCG TTGGGAAGGA GACTGTTAAA 
TATGAGGAGG AGCTGGATCT GCACGATGAA GAAGAGACTA GTGTTCCAGG AAGACCGGGC 
TCCACGAAGC GAAGGCAGTG CTACCCGAAA GCAATCCCCG AATGTTTGAG GGAGAGCTAC 
CCCAAGCCCG GTCAGCCCTG TTACCTGTAC GTGATCGGCA TGGTCCTGAC GACGCCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CCGAGGACAC CACGAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATTACAAG ACTTCATCAG TATATATTCT CGCATATTCT TCGGCTTGAA 
AAACCTGCCC TAGAATTTAA ACCCGCAGAC GCGGACTCAG CCTACTGTGT TCTACCTCTT 
AACGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGCATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCCTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTTAA TCTTTTGACA 
CCTCGCCATT TGAATCAGAA GGGGAAAGCT CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AAAGCCTGCA GAATAAACAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTCTGTCTCC CCAGCATACT CTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACAG CCAGCGATGC TGGTGTGGGA 
GTCAGATCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAGTTGCT AACTCCTCTT CAGCAGAAAA TGAGAATTAC 
TGTAAGCATA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGCTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAGTTGA AGTTGCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCCGCC 
GTGCCTGGTA CTACAGAGGC TGGGGAGGCG CCCCCGGACA GGACGGCTTC TGAGCAGAGC 
CCTTCTCCTG GGCACTCCTC GAGGACGCTC GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
CTGACCCTTT CGAATGCCAG CGACGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTGA AGCACGCCAT CACCACGTAT CTCTTCTGCA CGTACCCCGA CGCGCACGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAGCTGG ACGATGACTT TGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCCCAAGG AGGACGCCGA CGATGAGGAC 
GACTTCCTGG AGTACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT CCTTTTTCCG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTGGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCGTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC TGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGCGT CGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA GAGCATAGCT 
GACTGCGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGCG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTATGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ATGCAGACAG GACGCTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAACACCA TCTTCGCGTC GTTGGCTGTG AAATATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA CGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC CGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTTGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014944188.1
CDS	3863..9631
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRSGRRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVTASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILRSNAETATDLVVVDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCERLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPADADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISVANSSSAENENYCKHSTIVVPENAAHQGANRTSSLENHDQMSVNCRTLFSESPGKLQVEVATDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTEAGEAPPDRTASEQSPSPGHSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAPKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRMAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_015088702.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon dicer 1, ribonuclease type III (DICER1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015088702.1

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTGCTGCT CACTAAAGAG 
CTGTCTTACC AGATCAGGGG AGACTTCAAC AGAAGTGGCA GGAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAACT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC GTCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTT AGGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCGTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTA GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTACAG AAACACATCA AACACGAGCA AGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTAAGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CACCTGCCTC CCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAGTA CAAGCCGTAC GAGCGGCAGC AGTTTGAAAG CGTGGAATGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGTG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC ATTTACCAAT 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CGGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT TGATCTGCCC 
ACAGAGTATC GATCCTATGT TCAGTCTAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGATACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCAG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGACGA TGACGATGTG TTCCCACCAT ACGTGCTGAG GCCTGAGGAC 
GGTCCTCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG ATTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAGA 
CTGCACAAAA TTGGTGAACT GGATGACCAT TTGATGCCCG TTGGGAAGGA GACTGTTAAA 
TATGAGGAGG AGCTGGATCT GCACGATGAA GAAGAGACTA GTGTTCCAGG AAGACCGGGC 
TCCACGAAGC GAAGGCAGTG CTACCCGAAA GCAATCCCCG AATGTTTGAG GGAGAGCTAC 
CCCAAGCCCG GTCAGCCCTG TTACCTGTAC GTGATCGGCA TGGTCCTGAC GACGCCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CCGAGGACAC CACGAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATTACAAG ACTTCATCAG TATATATTCT CGCATATTCT TCGGCTTGAA 
AAACCTGCCC TAGAATTTAA ACCCGCAGAC GCGGACTCAG CCTACTGTGT TCTACCTCTT 
AACGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGCATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCCTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTTAA TCTTTTGACA 
CCTCGCCATT TGAATCAGAA GGGGAAAGCT CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AAAGCCTGCA GAATAAACAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTCTGTCTCC CCAGCATACT CTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACAG CCAGCGATGC TGGTGTGGGA 
GTCAGATCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAGTTGCT AACTCCTCTT CAGCAGAAAA TGAGAATTAC 
TGTAAGCATA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGCTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAGTTGA AGTTGCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCCGCC 
GTGCCTGGTA CTACAGAGGC TGGGGAGGCG CCCCCGGACA GGACGGCTTC TGAGCAGAGC 
CCTTCTCCTG GGCACTCCTC GAGGACGCTC GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
CTGACCCTTT CGAATGCCAG CGACGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTGA AGCACGCCAT CACCACGTAT CTCTTCTGCA CGTACCCCGA CGCGCACGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAGCTGG ACGATGACTT TGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCCCAAGG AGGACGCCGA CGATGAGGAC 
GACTTCCTGG AGTACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT CCTTTTTCCG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTGGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCGTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC TGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGCGT CGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA GAGCATAGCT 
GACTGCGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGCG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTATGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ATGCAGACAG GACGCTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAACACCA TCTTCGCGTC GTTGGCTGTG AAATATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA CGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC CGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTTGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_012112872.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011968262.1 Ovis aries musimon dicer 1, ribonuclease type III (DICER1), transcript variant X9, mRNA.

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CloneID	OOv02800
Clone ID Related Accession (Same CDS sequence)	XM_012112869.2 , XM_012112871.2 , XM_012112870.2 , XM_015088696.1 , XM_015088702.1 , XM_012112872.2
Accession Version	XM_012112872.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942251.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012112872.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTGCTGCT CACTAAAGAG 
CTGTCTTACC AGATCAGGGG AGACTTCAAC AGAAGTGGCA GGAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAACT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC GTCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTT AGGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCGTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTA GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTACAG AAACACATCA AACACGAGCA AGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTAAGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CACCTGCCTC CCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAGTA CAAGCCGTAC GAGCGGCAGC AGTTTGAAAG CGTGGAATGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGTG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC ATTTACCAAT 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CGGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT TGATCTGCCC 
ACAGAGTATC GATCCTATGT TCAGTCTAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGATACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCAG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGACGA TGACGATGTG TTCCCACCAT ACGTGCTGAG GCCTGAGGAC 
GGTCCTCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG ATTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAGA 
CTGCACAAAA TTGGTGAACT GGATGACCAT TTGATGCCCG TTGGGAAGGA GACTGTTAAA 
TATGAGGAGG AGCTGGATCT GCACGATGAA GAAGAGACTA GTGTTCCAGG AAGACCGGGC 
TCCACGAAGC GAAGGCAGTG CTACCCGAAA GCAATCCCCG AATGTTTGAG GGAGAGCTAC 
CCCAAGCCCG GTCAGCCCTG TTACCTGTAC GTGATCGGCA TGGTCCTGAC GACGCCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CCGAGGACAC CACGAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATTACAAG ACTTCATCAG TATATATTCT CGCATATTCT TCGGCTTGAA 
AAACCTGCCC TAGAATTTAA ACCCGCAGAC GCGGACTCAG CCTACTGTGT TCTACCTCTT 
AACGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGCATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCCTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTTAA TCTTTTGACA 
CCTCGCCATT TGAATCAGAA GGGGAAAGCT CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AAAGCCTGCA GAATAAACAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTCTGTCTCC CCAGCATACT CTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACAG CCAGCGATGC TGGTGTGGGA 
GTCAGATCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAGTTGCT AACTCCTCTT CAGCAGAAAA TGAGAATTAC 
TGTAAGCATA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGCTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAGTTGA AGTTGCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCCGCC 
GTGCCTGGTA CTACAGAGGC TGGGGAGGCG CCCCCGGACA GGACGGCTTC TGAGCAGAGC 
CCTTCTCCTG GGCACTCCTC GAGGACGCTC GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
CTGACCCTTT CGAATGCCAG CGACGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTGA AGCACGCCAT CACCACGTAT CTCTTCTGCA CGTACCCCGA CGCGCACGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAGCTGG ACGATGACTT TGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCCCAAGG AGGACGCCGA CGATGAGGAC 
GACTTCCTGG AGTACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT CCTTTTTCCG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTGGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCGTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC TGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGCGT CGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA GAGCATAGCT 
GACTGCGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGCG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTATGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ATGCAGACAG GACGCTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAACACCA TCTTCGCGTC GTTGGCTGTG AAATATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA CGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC CGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTTGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011968262.1
CDS	340..6108
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRSGRRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVTASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILRSNAETATDLVVVDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCERLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPADADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISVANSSSAENENYCKHSTIVVPENAAHQGANRTSSLENHDQMSVNCRTLFSESPGKLQVEVATDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTEAGEAPPDRTASEQSPSPGHSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAPKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRMAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_012112872.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon dicer 1, ribonuclease type III (DICER1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012112872.2

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTGCTGCT CACTAAAGAG 
CTGTCTTACC AGATCAGGGG AGACTTCAAC AGAAGTGGCA GGAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAACT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC GTCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTT AGGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCGTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTA GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTACAG AAACACATCA AACACGAGCA AGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTAAGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CACCTGCCTC CCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAGTA CAAGCCGTAC GAGCGGCAGC AGTTTGAAAG CGTGGAATGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGTG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC ATTTACCAAT 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CGGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT TGATCTGCCC 
ACAGAGTATC GATCCTATGT TCAGTCTAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGATACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCAG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGACGA TGACGATGTG TTCCCACCAT ACGTGCTGAG GCCTGAGGAC 
GGTCCTCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG ATTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAGA 
CTGCACAAAA TTGGTGAACT GGATGACCAT TTGATGCCCG TTGGGAAGGA GACTGTTAAA 
TATGAGGAGG AGCTGGATCT GCACGATGAA GAAGAGACTA GTGTTCCAGG AAGACCGGGC 
TCCACGAAGC GAAGGCAGTG CTACCCGAAA GCAATCCCCG AATGTTTGAG GGAGAGCTAC 
CCCAAGCCCG GTCAGCCCTG TTACCTGTAC GTGATCGGCA TGGTCCTGAC GACGCCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CCGAGGACAC CACGAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATTACAAG ACTTCATCAG TATATATTCT CGCATATTCT TCGGCTTGAA 
AAACCTGCCC TAGAATTTAA ACCCGCAGAC GCGGACTCAG CCTACTGTGT TCTACCTCTT 
AACGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGCATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCCTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTTAA TCTTTTGACA 
CCTCGCCATT TGAATCAGAA GGGGAAAGCT CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AAAGCCTGCA GAATAAACAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTCTGTCTCC CCAGCATACT CTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACAG CCAGCGATGC TGGTGTGGGA 
GTCAGATCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAGTTGCT AACTCCTCTT CAGCAGAAAA TGAGAATTAC 
TGTAAGCATA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGCTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAGTTGA AGTTGCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCCGCC 
GTGCCTGGTA CTACAGAGGC TGGGGAGGCG CCCCCGGACA GGACGGCTTC TGAGCAGAGC 
CCTTCTCCTG GGCACTCCTC GAGGACGCTC GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
CTGACCCTTT CGAATGCCAG CGACGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTGA AGCACGCCAT CACCACGTAT CTCTTCTGCA CGTACCCCGA CGCGCACGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAGCTGG ACGATGACTT TGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCCCAAGG AGGACGCCGA CGATGAGGAC 
GACTTCCTGG AGTACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT CCTTTTTCCG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTGGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCGTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC TGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGCGT CGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA GAGCATAGCT 
GACTGCGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGCG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTATGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ATGCAGACAG GACGCTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAACACCA TCTTCGCGTC GTTGGCTGTG AAATATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA CGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC CGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTTGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_012112873.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011968263.1 Ovis aries musimon dicer 1, ribonuclease type III (DICER1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OOv02801
Clone ID Related Accession (Same CDS sequence)	XM_012112873.2 , XM_015088705.1 , XM_015088703.1
Accession Version	XM_012112873.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	endoribonuclease Dicer isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942251.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012112873.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAAT GCAGTTCAGA CAACAGCAGC ACAGAGATCT CAAACATTAA GACGGTTGAA 
CTGCTTGAAG CAGCTCTGGA TCATAATACC ATAGTCTGTT TAAACACTGG CTCAGGGAAG 
ACGTTTATTG CAGTGCTGCT CACTAAAGAG CTGTCTTACC AGATCAGGGG AGACTTCAAC 
AGAAGTGGCA GGAGGACGGT GTTCTTGGTC AACTCTGCAA ACCAGGTTGC TCAACAAGTG 
TCAGCTGTCA GAACTCACTC AGATCTCAAG GTCGGGGAAT ACTCAAACCT AGAAGTAACT 
GCATCTTGGA CAAAAGAGAA ATGGAACCAA GAGTTTACTA AACATCAGGT TCTCGTTATG 
ACTTGCTATG TCGCCTTGAA TGTTTTGAAA AATGGTTACT TATCACTGTC AGACATTAAC 
CTTTTGGTGT TTGATGAGTG TCATCTTGCA ATCCTAGACC ACCCCTACCG AGAAATTATG 
AAGCTTTGTG AAAATTGTCC GTCATGTCCT CGTATTTTGG GACTAACTGC TTCCATTTTA 
AATGGGAAAT GTGATCCAGA GGAATTGGAA GAAAAGATTC AGAAACTGGA GAAAATTCTT 
AGGAGTAATG CTGAAACTGC AACTGACTTG GTGGTCGTAG ACAGATATAC TTCTCAGCCA 
TGTGAGATTG TGGTAGACTG TGGACCATTT ACTGACAGAA GTGGGCTTTA TGAAAGACTG 
CTGATGGAGT TAGAAGAAGC ACTTAATTTT ATCAATGACT GTAACATATC TGTACATTCA 
AAAGAAAGAG ATTCTACTTT AATTTCTAAA CAGATACTCT CAGACTGCCG TGCGGTCCTA 
GTTGTCCTGG GACCCTGGTG TGCCGATAAA GTAGCTGGAA TGATGGTCAG AGAGCTACAG 
AAACACATCA AACACGAGCA AGAGGAGCTG CACCGGAAGT TTCTGTTGTT CACAGACACT 
TTCCTAAGGA AAATCCACGC CCTGTGTGAA GAGCACTTCT CACCTGCCTC CCTTGACCTG 
AAGTTTGTCA CTCCTAAAGT AATAAAGCTG CTCGAGATCT TACGCAAGTA CAAGCCGTAC 
GAGCGGCAGC AGTTTGAAAG CGTGGAATGG TATAATAATA GGAACCAGGA TAATTACGTG 
TCCTGGAGTG ATTCTGAGGA TGACGAGGAA GATGAAGAGA TTGAAGAGAA AGAGAAGCCA 
GAGACAAATT TTCCTTCTCC ATTTACCAAT ATTTTGTGTG GAATTATTTT TGTGGAAAGA 
AGATACACAG CGGTGGTCTT AAACAGATTG ATAAAGGAAG CTGGCAAACA AGATCCAGAG 
CTGGCTTACA TCAGCAGCAA TTTTATAACT GGACATGGCA TTGGAAAGAA TCAGCCTCGG 
AACAAACAGA TGGAAGCGGA ATTCAGAAAA CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA 
CATGAAACCA ACCTGCTTAT TGCAACAAGT ATTGTGGAAG AGGGTGTTGA CATACCGAAA 
TGCAACTTGG TGGTTCGTTT TGATCTGCCC ACAGAGTATC GATCCTATGT TCAGTCTAAG 
GGAAGAGCGA GGGCACCCAT CTCTAATTAT GTAATGTTAG CAGATACAGA TAAAATAAAG 
AGTTTTGAAG AAGACCTTAA AACATACAAA GCTATTGAAA AGATCTTGCG AAACAAATGT 
TCCAAGTCAG TTGATACCGG GGAGGCCGAC ACGGAGCCCG TGGTGGACGA TGACGATGTG 
TTCCCACCAT ACGTGCTGAG GCCTGAGGAC GGTCCTCGCG TCACGATCAA CACGGCCATT 
GGGCACGTCA ACAGATACTG TGCTAGATTA CCAAGTGATC CGTTTACTCA TCTGGCTCCT 
AAATGTAGAA CCCGAGAGTT GCCTGATGGT ACATTTTATT CAACTCTTTA TCTGCCAATT 
AACTCACCTC TTCGAGCCTC CATTGTTGGT CCCCCAATGA GCTGCATTCG ATTGGCTGAA 
AGAGTCGTGG CTCTCATTTG CTGTGAAAGA CTGCACAAAA TTGGTGAACT GGATGACCAT 
TTGATGCCCG TTGGGAAGGA GACTGTTAAA TATGAGGAGG AGCTGGATCT GCACGATGAA 
GAAGAGACTA GTGTTCCAGG AAGACCGGGC TCCACGAAGC GAAGGCAGTG CTACCCGAAA 
GCAATCCCCG AATGTTTGAG GGAGAGCTAC CCCAAGCCCG GTCAGCCCTG TTACCTGTAC 
GTGATCGGCA TGGTCCTGAC GACGCCTTTA CCTGATGAGC TCAACTTCAG AAGGCGGAAG 
CTCTACCCCC CCGAGGACAC CACGAGATGC TTTGGAATAC TGACAGCCAA GCCCATACCT 
CAGATCCCAC ACTTTCCTGT GTACACGCGC TCTGGAGAGG TGACCATATC TATTGAGTTG 
AAGAAGTCCG GCTTCACGTT ATCTCTGCAA ATGCTTGAGT TGATTACAAG ACTTCATCAG 
TATATATTCT CGCATATTCT TCGGCTTGAA AAACCTGCCC TAGAATTTAA ACCCGCAGAC 
GCGGACTCAG CCTACTGTGT TCTACCTCTT AACGTTGTTA ATGACTCCAG CACTTTGGAC 
ATTGACTTTA AATTCATGGA AGATATCGAG AAATCTGAAG CTCGCATAGG CATTCCCAGC 
ACAAAGTATT CAAAAGAAAC TCCCTTTGTT TTCAAATTAG AAGATTACCA AGATGCAGTT 
ATCATTCCAA GATACCGCAA TTTTGATCAG CCTCATCGAT TTTATGTCGC TGACGTGTAC 
ACTGATCTTA CCCCCCTGAG TAAATTTCCT TCCCCTGAGT ATGAAACGTT TGCGGAGTAT 
TATAAAACAA AGTATAACCT TGACCTGACC AATCTCAACC AGCCGCTGCT GGACGTGGAC 
CACACGTCTT CACGACTTAA TCTTTTGACA CCTCGCCATT TGAATCAGAA GGGGAAAGCT 
CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA GCCAAGTGGG AAAGCCTGCA GAATAAACAG 
ATACTGGTTC CAGAACTCTG TGCTATACAT CCAATTCCAG CATCACTGTG GAGAAAAGCC 
GTCTGTCTCC CCAGCATACT CTACCGCCTT CACTGCCTTC TGACTGCAGA GGAGCTCAGA 
GCCCAGACAG CCAGCGATGC TGGTGTGGGA GTCAGATCAC TTCCTGTGGA TTTTAGATAC 
CCTAACTTAG ACTTCGGGTG GAAAAAATCT ATCGACAGCA AATCTTTCAT CTCAGTTGCT 
AACTCCTCTT CAGCAGAAAA TGAGAATTAC TGTAAGCATA GCACAATTGT CGTCCCTGAA 
AATGCTGCAC ATCAAGGTGC TAATAGAACC TCCTCTCTAG AAAATCATGA CCAAATGTCT 
GTGAACTGCA GAACGCTGTT CAGCGAGTCA CCTGGTAAGC TCCAAGTTGA AGTTGCAACA 
GATCTGACAG CAATTAATGG TCTTTCTTAC AATAAAAGTC TTGCCAATGG CAGTTACGAT 
TTAGCTAACA GAGACTTTTG CCAAGGAAAC CATCTGAATT ACTACAAGCA GGAAATACCT 
GTACAACCAA CTACCTCATA TCCCATTCAG AATTTATACA ATTACGAGAA CCAGCCCAAG 
CCCAGCGATG AATGTACTCT ACTGAGTAAT AAATACCTTG ATGGAAATGC TAACACATCT 
ACCTCAGATG GAAGTCCCGT GACGGCCGCC GTGCCTGGTA CTACAGAGGC TGGGGAGGCG 
CCCCCGGACA GGACGGCTTC TGAGCAGAGC CCTTCTCCTG GGCACTCCTC GAGGACGCTC 
GGCCCCAACC CTGGGCTCAT TCTTCAGGCT CTGACCCTTT CGAATGCCAG CGACGGATTT 
AACCTGGAGC GGCTCGAGAT GCTTGGCGAC TCCTTCCTGA AGCACGCCAT CACCACGTAT 
CTCTTCTGCA CGTACCCCGA CGCGCACGAG GGCCGCCTTT CCTACATGAG AAGCAAAAAG 
GTCAGCAACT GTAATCTGTA TCGGCTTGGA AAAAAGAAGG GGCTGCCCAG CCGCATGGTG 
GTGTCCATAT TTGACCCCCC TGTGAATTGG CTTCCTCCTG GTTACGTAGT AAATCAAGAC 
AAAAGTAACA CAGAGAAATG GGAAAAAGAT GAAATGACAA AAGACTGCAT GTTGGCTAAT 
GGCAAGCTGG ACGATGACTT TGAGGAGGAG GAAGAGGAGG AGGAGGACCT GATGTGGAGG 
GCTCCCAAGG AGGACGCCGA CGATGAGGAC GACTTCCTGG AGTACGATCA GGAGCACATC 
AAGTTCATAG ATAACATGCT GATGGGGTCA GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT 
CCTTTTTCCG CCACCGATTC TGCATACGAG TGGAAAATGC CCAAAAAGTC CTCCCTGGGT 
AGTCTGCCAT TCTCATCCGA TTTTGAGGAT TTCGACTACA GCTCGTGGGA TGCGATGTGC 
TATCTGGATC CGAGCAAAGC TGTTGAAGAG GATGACTTTG TGGTGGGGTT CTGGAATCCA 
TCAGAAGAAA ACTGTGGCGT CGACACAGGG AAGCAGTCCA TCTCTTACGA CCTGCACACC 
GAGCAGTGCA TTGCCGACAA GAGCATAGCT GACTGCGTGG AAGCCCTGCT GGGCTGCTAT 
TTAACCAGCT GTGGCGAGCG GGCTGCTCAG CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG 
CTCCCGGTAA TTAAAAGGAC TGATCGGGAA AAGGCCATGT GCCCGACCAG GGAGAATTTC 
ACCAGCCAAC AAAAGAACCT TTCAGGGAGC CGTATGGCCG CCTCTGGAGC CGGCTACCGA 
GCCTCTGTGT TGAAAGACTT GGAGTATGGG TGTTTGAAGA TCCCCCCGAG GTGTATGTTT 
GATCACCCCG ATGCAGACAG GACGCTGCGT CACCTGATCT CGGGTTTTGA AAATTTCGAA 
AAGAAAATCA ACTACAGATT CAAGAATAAG GCTTACCTTC TACAGGCTTT TACACACGCC 
TCCTACCACT ACAATACGAT CACGGATTGT TACCAGCGCT TAGAATTCCT GGGAGATGCA 
ATTTTGGACT ACCTCATAAC CAAGCACCTT TATGAAGACC CACGGCAGCA CTCCCCAGGG 
GTCCTGACTG ACCTGCGCTC TGCTCTGGTC AACAACACCA TCTTCGCGTC GTTGGCTGTG 
AAATATGACT ACCACAAGTA CTTTAAGGCC GTTTCTCCCG AGCTCTTCCA CGTCATCGAC 
GATTTCGTGC AGTTTCAGCT CGAGAAGAAC GAAATGCAAG GAATGGATTC CGAGCTTAGG 
AGATCCGAGG AGGATGAAGA GAAAGAAGAG GATATTGAAG TTCCAAAGGC CATGGGGGAT 
ATTTTCGAGT CACTTGCGGG CGCCATTTAC ATGGACAGCG GGATGTCGCT GGAGACGGTC 
TGGCAGGTGT ACTATCCCAT GATGCGGCCG CTGATCGAAA AATTTTCTGC AAATGTGCCC 
CGTTCCCCTG TGCGAGAATT GCTTGAAATG GAACCAGAAA CTGCCAAATT CAGCCCAGCT 
GAGAGAACCT ACGATGGCAA GGTCAGAGTC ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC 
AAAGGCGTTG GCCGAAGTTA CAGGATTGCC AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA 
AGCCTCAAAG CTAATCAACC TCAGGTTCCC AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011968263.1
CDS	406..6084
Translation	MSKCSSDNSSTEISNIKTVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRSGRRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVTASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILRSNAETATDLVVVDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCERLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPADADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISVANSSSAENENYCKHSTIVVPENAAHQGANRTSSLENHDQMSVNCRTLFSESPGKLQVEVATDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTEAGEAPPDRTASEQSPSPGHSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAPKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRMAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_012112873.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon dicer 1, ribonuclease type III (DICER1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012112873.2

ORF Insert Sequence:

ATGTCAAAAT GCAGTTCAGA CAACAGCAGC ACAGAGATCT CAAACATTAA GACGGTTGAA 
CTGCTTGAAG CAGCTCTGGA TCATAATACC ATAGTCTGTT TAAACACTGG CTCAGGGAAG 
ACGTTTATTG CAGTGCTGCT CACTAAAGAG CTGTCTTACC AGATCAGGGG AGACTTCAAC 
AGAAGTGGCA GGAGGACGGT GTTCTTGGTC AACTCTGCAA ACCAGGTTGC TCAACAAGTG 
TCAGCTGTCA GAACTCACTC AGATCTCAAG GTCGGGGAAT ACTCAAACCT AGAAGTAACT 
GCATCTTGGA CAAAAGAGAA ATGGAACCAA GAGTTTACTA AACATCAGGT TCTCGTTATG 
ACTTGCTATG TCGCCTTGAA TGTTTTGAAA AATGGTTACT TATCACTGTC AGACATTAAC 
CTTTTGGTGT TTGATGAGTG TCATCTTGCA ATCCTAGACC ACCCCTACCG AGAAATTATG 
AAGCTTTGTG AAAATTGTCC GTCATGTCCT CGTATTTTGG GACTAACTGC TTCCATTTTA 
AATGGGAAAT GTGATCCAGA GGAATTGGAA GAAAAGATTC AGAAACTGGA GAAAATTCTT 
AGGAGTAATG CTGAAACTGC AACTGACTTG GTGGTCGTAG ACAGATATAC TTCTCAGCCA 
TGTGAGATTG TGGTAGACTG TGGACCATTT ACTGACAGAA GTGGGCTTTA TGAAAGACTG 
CTGATGGAGT TAGAAGAAGC ACTTAATTTT ATCAATGACT GTAACATATC TGTACATTCA 
AAAGAAAGAG ATTCTACTTT AATTTCTAAA CAGATACTCT CAGACTGCCG TGCGGTCCTA 
GTTGTCCTGG GACCCTGGTG TGCCGATAAA GTAGCTGGAA TGATGGTCAG AGAGCTACAG 
AAACACATCA AACACGAGCA AGAGGAGCTG CACCGGAAGT TTCTGTTGTT CACAGACACT 
TTCCTAAGGA AAATCCACGC CCTGTGTGAA GAGCACTTCT CACCTGCCTC CCTTGACCTG 
AAGTTTGTCA CTCCTAAAGT AATAAAGCTG CTCGAGATCT TACGCAAGTA CAAGCCGTAC 
GAGCGGCAGC AGTTTGAAAG CGTGGAATGG TATAATAATA GGAACCAGGA TAATTACGTG 
TCCTGGAGTG ATTCTGAGGA TGACGAGGAA GATGAAGAGA TTGAAGAGAA AGAGAAGCCA 
GAGACAAATT TTCCTTCTCC ATTTACCAAT ATTTTGTGTG GAATTATTTT TGTGGAAAGA 
AGATACACAG CGGTGGTCTT AAACAGATTG ATAAAGGAAG CTGGCAAACA AGATCCAGAG 
CTGGCTTACA TCAGCAGCAA TTTTATAACT GGACATGGCA TTGGAAAGAA TCAGCCTCGG 
AACAAACAGA TGGAAGCGGA ATTCAGAAAA CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA 
CATGAAACCA ACCTGCTTAT TGCAACAAGT ATTGTGGAAG AGGGTGTTGA CATACCGAAA 
TGCAACTTGG TGGTTCGTTT TGATCTGCCC ACAGAGTATC GATCCTATGT TCAGTCTAAG 
GGAAGAGCGA GGGCACCCAT CTCTAATTAT GTAATGTTAG CAGATACAGA TAAAATAAAG 
AGTTTTGAAG AAGACCTTAA AACATACAAA GCTATTGAAA AGATCTTGCG AAACAAATGT 
TCCAAGTCAG TTGATACCGG GGAGGCCGAC ACGGAGCCCG TGGTGGACGA TGACGATGTG 
TTCCCACCAT ACGTGCTGAG GCCTGAGGAC GGTCCTCGCG TCACGATCAA CACGGCCATT 
GGGCACGTCA ACAGATACTG TGCTAGATTA CCAAGTGATC CGTTTACTCA TCTGGCTCCT 
AAATGTAGAA CCCGAGAGTT GCCTGATGGT ACATTTTATT CAACTCTTTA TCTGCCAATT 
AACTCACCTC TTCGAGCCTC CATTGTTGGT CCCCCAATGA GCTGCATTCG ATTGGCTGAA 
AGAGTCGTGG CTCTCATTTG CTGTGAAAGA CTGCACAAAA TTGGTGAACT GGATGACCAT 
TTGATGCCCG TTGGGAAGGA GACTGTTAAA TATGAGGAGG AGCTGGATCT GCACGATGAA 
GAAGAGACTA GTGTTCCAGG AAGACCGGGC TCCACGAAGC GAAGGCAGTG CTACCCGAAA 
GCAATCCCCG AATGTTTGAG GGAGAGCTAC CCCAAGCCCG GTCAGCCCTG TTACCTGTAC 
GTGATCGGCA TGGTCCTGAC GACGCCTTTA CCTGATGAGC TCAACTTCAG AAGGCGGAAG 
CTCTACCCCC CCGAGGACAC CACGAGATGC TTTGGAATAC TGACAGCCAA GCCCATACCT 
CAGATCCCAC ACTTTCCTGT GTACACGCGC TCTGGAGAGG TGACCATATC TATTGAGTTG 
AAGAAGTCCG GCTTCACGTT ATCTCTGCAA ATGCTTGAGT TGATTACAAG ACTTCATCAG 
TATATATTCT CGCATATTCT TCGGCTTGAA AAACCTGCCC TAGAATTTAA ACCCGCAGAC 
GCGGACTCAG CCTACTGTGT TCTACCTCTT AACGTTGTTA ATGACTCCAG CACTTTGGAC 
ATTGACTTTA AATTCATGGA AGATATCGAG AAATCTGAAG CTCGCATAGG CATTCCCAGC 
ACAAAGTATT CAAAAGAAAC TCCCTTTGTT TTCAAATTAG AAGATTACCA AGATGCAGTT 
ATCATTCCAA GATACCGCAA TTTTGATCAG CCTCATCGAT TTTATGTCGC TGACGTGTAC 
ACTGATCTTA CCCCCCTGAG TAAATTTCCT TCCCCTGAGT ATGAAACGTT TGCGGAGTAT 
TATAAAACAA AGTATAACCT TGACCTGACC AATCTCAACC AGCCGCTGCT GGACGTGGAC 
CACACGTCTT CACGACTTAA TCTTTTGACA CCTCGCCATT TGAATCAGAA GGGGAAAGCT 
CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA GCCAAGTGGG AAAGCCTGCA GAATAAACAG 
ATACTGGTTC CAGAACTCTG TGCTATACAT CCAATTCCAG CATCACTGTG GAGAAAAGCC 
GTCTGTCTCC CCAGCATACT CTACCGCCTT CACTGCCTTC TGACTGCAGA GGAGCTCAGA 
GCCCAGACAG CCAGCGATGC TGGTGTGGGA GTCAGATCAC TTCCTGTGGA TTTTAGATAC 
CCTAACTTAG ACTTCGGGTG GAAAAAATCT ATCGACAGCA AATCTTTCAT CTCAGTTGCT 
AACTCCTCTT CAGCAGAAAA TGAGAATTAC TGTAAGCATA GCACAATTGT CGTCCCTGAA 
AATGCTGCAC ATCAAGGTGC TAATAGAACC TCCTCTCTAG AAAATCATGA CCAAATGTCT 
GTGAACTGCA GAACGCTGTT CAGCGAGTCA CCTGGTAAGC TCCAAGTTGA AGTTGCAACA 
GATCTGACAG CAATTAATGG TCTTTCTTAC AATAAAAGTC TTGCCAATGG CAGTTACGAT 
TTAGCTAACA GAGACTTTTG CCAAGGAAAC CATCTGAATT ACTACAAGCA GGAAATACCT 
GTACAACCAA CTACCTCATA TCCCATTCAG AATTTATACA ATTACGAGAA CCAGCCCAAG 
CCCAGCGATG AATGTACTCT ACTGAGTAAT AAATACCTTG ATGGAAATGC TAACACATCT 
ACCTCAGATG GAAGTCCCGT GACGGCCGCC GTGCCTGGTA CTACAGAGGC TGGGGAGGCG 
CCCCCGGACA GGACGGCTTC TGAGCAGAGC CCTTCTCCTG GGCACTCCTC GAGGACGCTC 
GGCCCCAACC CTGGGCTCAT TCTTCAGGCT CTGACCCTTT CGAATGCCAG CGACGGATTT 
AACCTGGAGC GGCTCGAGAT GCTTGGCGAC TCCTTCCTGA AGCACGCCAT CACCACGTAT 
CTCTTCTGCA CGTACCCCGA CGCGCACGAG GGCCGCCTTT CCTACATGAG AAGCAAAAAG 
GTCAGCAACT GTAATCTGTA TCGGCTTGGA AAAAAGAAGG GGCTGCCCAG CCGCATGGTG 
GTGTCCATAT TTGACCCCCC TGTGAATTGG CTTCCTCCTG GTTACGTAGT AAATCAAGAC 
AAAAGTAACA CAGAGAAATG GGAAAAAGAT GAAATGACAA AAGACTGCAT GTTGGCTAAT 
GGCAAGCTGG ACGATGACTT TGAGGAGGAG GAAGAGGAGG AGGAGGACCT GATGTGGAGG 
GCTCCCAAGG AGGACGCCGA CGATGAGGAC GACTTCCTGG AGTACGATCA GGAGCACATC 
AAGTTCATAG ATAACATGCT GATGGGGTCA GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT 
CCTTTTTCCG CCACCGATTC TGCATACGAG TGGAAAATGC CCAAAAAGTC CTCCCTGGGT 
AGTCTGCCAT TCTCATCCGA TTTTGAGGAT TTCGACTACA GCTCGTGGGA TGCGATGTGC 
TATCTGGATC CGAGCAAAGC TGTTGAAGAG GATGACTTTG TGGTGGGGTT CTGGAATCCA 
TCAGAAGAAA ACTGTGGCGT CGACACAGGG AAGCAGTCCA TCTCTTACGA CCTGCACACC 
GAGCAGTGCA TTGCCGACAA GAGCATAGCT GACTGCGTGG AAGCCCTGCT GGGCTGCTAT 
TTAACCAGCT GTGGCGAGCG GGCTGCTCAG CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG 
CTCCCGGTAA TTAAAAGGAC TGATCGGGAA AAGGCCATGT GCCCGACCAG GGAGAATTTC 
ACCAGCCAAC AAAAGAACCT TTCAGGGAGC CGTATGGCCG CCTCTGGAGC CGGCTACCGA 
GCCTCTGTGT TGAAAGACTT GGAGTATGGG TGTTTGAAGA TCCCCCCGAG GTGTATGTTT 
GATCACCCCG ATGCAGACAG GACGCTGCGT CACCTGATCT CGGGTTTTGA AAATTTCGAA 
AAGAAAATCA ACTACAGATT CAAGAATAAG GCTTACCTTC TACAGGCTTT TACACACGCC 
TCCTACCACT ACAATACGAT CACGGATTGT TACCAGCGCT TAGAATTCCT GGGAGATGCA 
ATTTTGGACT ACCTCATAAC CAAGCACCTT TATGAAGACC CACGGCAGCA CTCCCCAGGG 
GTCCTGACTG ACCTGCGCTC TGCTCTGGTC AACAACACCA TCTTCGCGTC GTTGGCTGTG 
AAATATGACT ACCACAAGTA CTTTAAGGCC GTTTCTCCCG AGCTCTTCCA CGTCATCGAC 
GATTTCGTGC AGTTTCAGCT CGAGAAGAAC GAAATGCAAG GAATGGATTC CGAGCTTAGG 
AGATCCGAGG AGGATGAAGA GAAAGAAGAG GATATTGAAG TTCCAAAGGC CATGGGGGAT 
ATTTTCGAGT CACTTGCGGG CGCCATTTAC ATGGACAGCG GGATGTCGCT GGAGACGGTC 
TGGCAGGTGT ACTATCCCAT GATGCGGCCG CTGATCGAAA AATTTTCTGC AAATGTGCCC 
CGTTCCCCTG TGCGAGAATT GCTTGAAATG GAACCAGAAA CTGCCAAATT CAGCCCAGCT 
GAGAGAACCT ACGATGGCAA GGTCAGAGTC ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC 
AAAGGCGTTG GCCGAAGTTA CAGGATTGCC AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA 
AGCCTCAAAG CTAATCAACC TCAGGTTCCC AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_015088705.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014944191.1 Ovis aries musimon dicer 1, ribonuclease type III (DICER1), transcript variant X12, mRNA.

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CloneID	OOv02801
Clone ID Related Accession (Same CDS sequence)	XM_012112873.2 , XM_015088705.1 , XM_015088703.1
Accession Version	XM_015088705.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	endoribonuclease Dicer isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942251.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAAT GCAGTTCAGA CAACAGCAGC ACAGAGATCT CAAACATTAA GACGGTTGAA 
CTGCTTGAAG CAGCTCTGGA TCATAATACC ATAGTCTGTT TAAACACTGG CTCAGGGAAG 
ACGTTTATTG CAGTGCTGCT CACTAAAGAG CTGTCTTACC AGATCAGGGG AGACTTCAAC 
AGAAGTGGCA GGAGGACGGT GTTCTTGGTC AACTCTGCAA ACCAGGTTGC TCAACAAGTG 
TCAGCTGTCA GAACTCACTC AGATCTCAAG GTCGGGGAAT ACTCAAACCT AGAAGTAACT 
GCATCTTGGA CAAAAGAGAA ATGGAACCAA GAGTTTACTA AACATCAGGT TCTCGTTATG 
ACTTGCTATG TCGCCTTGAA TGTTTTGAAA AATGGTTACT TATCACTGTC AGACATTAAC 
CTTTTGGTGT TTGATGAGTG TCATCTTGCA ATCCTAGACC ACCCCTACCG AGAAATTATG 
AAGCTTTGTG AAAATTGTCC GTCATGTCCT CGTATTTTGG GACTAACTGC TTCCATTTTA 
AATGGGAAAT GTGATCCAGA GGAATTGGAA GAAAAGATTC AGAAACTGGA GAAAATTCTT 
AGGAGTAATG CTGAAACTGC AACTGACTTG GTGGTCGTAG ACAGATATAC TTCTCAGCCA 
TGTGAGATTG TGGTAGACTG TGGACCATTT ACTGACAGAA GTGGGCTTTA TGAAAGACTG 
CTGATGGAGT TAGAAGAAGC ACTTAATTTT ATCAATGACT GTAACATATC TGTACATTCA 
AAAGAAAGAG ATTCTACTTT AATTTCTAAA CAGATACTCT CAGACTGCCG TGCGGTCCTA 
GTTGTCCTGG GACCCTGGTG TGCCGATAAA GTAGCTGGAA TGATGGTCAG AGAGCTACAG 
AAACACATCA AACACGAGCA AGAGGAGCTG CACCGGAAGT TTCTGTTGTT CACAGACACT 
TTCCTAAGGA AAATCCACGC CCTGTGTGAA GAGCACTTCT CACCTGCCTC CCTTGACCTG 
AAGTTTGTCA CTCCTAAAGT AATAAAGCTG CTCGAGATCT TACGCAAGTA CAAGCCGTAC 
GAGCGGCAGC AGTTTGAAAG CGTGGAATGG TATAATAATA GGAACCAGGA TAATTACGTG 
TCCTGGAGTG ATTCTGAGGA TGACGAGGAA GATGAAGAGA TTGAAGAGAA AGAGAAGCCA 
GAGACAAATT TTCCTTCTCC ATTTACCAAT ATTTTGTGTG GAATTATTTT TGTGGAAAGA 
AGATACACAG CGGTGGTCTT AAACAGATTG ATAAAGGAAG CTGGCAAACA AGATCCAGAG 
CTGGCTTACA TCAGCAGCAA TTTTATAACT GGACATGGCA TTGGAAAGAA TCAGCCTCGG 
AACAAACAGA TGGAAGCGGA ATTCAGAAAA CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA 
CATGAAACCA ACCTGCTTAT TGCAACAAGT ATTGTGGAAG AGGGTGTTGA CATACCGAAA 
TGCAACTTGG TGGTTCGTTT TGATCTGCCC ACAGAGTATC GATCCTATGT TCAGTCTAAG 
GGAAGAGCGA GGGCACCCAT CTCTAATTAT GTAATGTTAG CAGATACAGA TAAAATAAAG 
AGTTTTGAAG AAGACCTTAA AACATACAAA GCTATTGAAA AGATCTTGCG AAACAAATGT 
TCCAAGTCAG TTGATACCGG GGAGGCCGAC ACGGAGCCCG TGGTGGACGA TGACGATGTG 
TTCCCACCAT ACGTGCTGAG GCCTGAGGAC GGTCCTCGCG TCACGATCAA CACGGCCATT 
GGGCACGTCA ACAGATACTG TGCTAGATTA CCAAGTGATC CGTTTACTCA TCTGGCTCCT 
AAATGTAGAA CCCGAGAGTT GCCTGATGGT ACATTTTATT CAACTCTTTA TCTGCCAATT 
AACTCACCTC TTCGAGCCTC CATTGTTGGT CCCCCAATGA GCTGCATTCG ATTGGCTGAA 
AGAGTCGTGG CTCTCATTTG CTGTGAAAGA CTGCACAAAA TTGGTGAACT GGATGACCAT 
TTGATGCCCG TTGGGAAGGA GACTGTTAAA TATGAGGAGG AGCTGGATCT GCACGATGAA 
GAAGAGACTA GTGTTCCAGG AAGACCGGGC TCCACGAAGC GAAGGCAGTG CTACCCGAAA 
GCAATCCCCG AATGTTTGAG GGAGAGCTAC CCCAAGCCCG GTCAGCCCTG TTACCTGTAC 
GTGATCGGCA TGGTCCTGAC GACGCCTTTA CCTGATGAGC TCAACTTCAG AAGGCGGAAG 
CTCTACCCCC CCGAGGACAC CACGAGATGC TTTGGAATAC TGACAGCCAA GCCCATACCT 
CAGATCCCAC ACTTTCCTGT GTACACGCGC TCTGGAGAGG TGACCATATC TATTGAGTTG 
AAGAAGTCCG GCTTCACGTT ATCTCTGCAA ATGCTTGAGT TGATTACAAG ACTTCATCAG 
TATATATTCT CGCATATTCT TCGGCTTGAA AAACCTGCCC TAGAATTTAA ACCCGCAGAC 
GCGGACTCAG CCTACTGTGT TCTACCTCTT AACGTTGTTA ATGACTCCAG CACTTTGGAC 
ATTGACTTTA AATTCATGGA AGATATCGAG AAATCTGAAG CTCGCATAGG CATTCCCAGC 
ACAAAGTATT CAAAAGAAAC TCCCTTTGTT TTCAAATTAG AAGATTACCA AGATGCAGTT 
ATCATTCCAA GATACCGCAA TTTTGATCAG CCTCATCGAT TTTATGTCGC TGACGTGTAC 
ACTGATCTTA CCCCCCTGAG TAAATTTCCT TCCCCTGAGT ATGAAACGTT TGCGGAGTAT 
TATAAAACAA AGTATAACCT TGACCTGACC AATCTCAACC AGCCGCTGCT GGACGTGGAC 
CACACGTCTT CACGACTTAA TCTTTTGACA CCTCGCCATT TGAATCAGAA GGGGAAAGCT 
CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA GCCAAGTGGG AAAGCCTGCA GAATAAACAG 
ATACTGGTTC CAGAACTCTG TGCTATACAT CCAATTCCAG CATCACTGTG GAGAAAAGCC 
GTCTGTCTCC CCAGCATACT CTACCGCCTT CACTGCCTTC TGACTGCAGA GGAGCTCAGA 
GCCCAGACAG CCAGCGATGC TGGTGTGGGA GTCAGATCAC TTCCTGTGGA TTTTAGATAC 
CCTAACTTAG ACTTCGGGTG GAAAAAATCT ATCGACAGCA AATCTTTCAT CTCAGTTGCT 
AACTCCTCTT CAGCAGAAAA TGAGAATTAC TGTAAGCATA GCACAATTGT CGTCCCTGAA 
AATGCTGCAC ATCAAGGTGC TAATAGAACC TCCTCTCTAG AAAATCATGA CCAAATGTCT 
GTGAACTGCA GAACGCTGTT CAGCGAGTCA CCTGGTAAGC TCCAAGTTGA AGTTGCAACA 
GATCTGACAG CAATTAATGG TCTTTCTTAC AATAAAAGTC TTGCCAATGG CAGTTACGAT 
TTAGCTAACA GAGACTTTTG CCAAGGAAAC CATCTGAATT ACTACAAGCA GGAAATACCT 
GTACAACCAA CTACCTCATA TCCCATTCAG AATTTATACA ATTACGAGAA CCAGCCCAAG 
CCCAGCGATG AATGTACTCT ACTGAGTAAT AAATACCTTG ATGGAAATGC TAACACATCT 
ACCTCAGATG GAAGTCCCGT GACGGCCGCC GTGCCTGGTA CTACAGAGGC TGGGGAGGCG 
CCCCCGGACA GGACGGCTTC TGAGCAGAGC CCTTCTCCTG GGCACTCCTC GAGGACGCTC 
GGCCCCAACC CTGGGCTCAT TCTTCAGGCT CTGACCCTTT CGAATGCCAG CGACGGATTT 
AACCTGGAGC GGCTCGAGAT GCTTGGCGAC TCCTTCCTGA AGCACGCCAT CACCACGTAT 
CTCTTCTGCA CGTACCCCGA CGCGCACGAG GGCCGCCTTT CCTACATGAG AAGCAAAAAG 
GTCAGCAACT GTAATCTGTA TCGGCTTGGA AAAAAGAAGG GGCTGCCCAG CCGCATGGTG 
GTGTCCATAT TTGACCCCCC TGTGAATTGG CTTCCTCCTG GTTACGTAGT AAATCAAGAC 
AAAAGTAACA CAGAGAAATG GGAAAAAGAT GAAATGACAA AAGACTGCAT GTTGGCTAAT 
GGCAAGCTGG ACGATGACTT TGAGGAGGAG GAAGAGGAGG AGGAGGACCT GATGTGGAGG 
GCTCCCAAGG AGGACGCCGA CGATGAGGAC GACTTCCTGG AGTACGATCA GGAGCACATC 
AAGTTCATAG ATAACATGCT GATGGGGTCA GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT 
CCTTTTTCCG CCACCGATTC TGCATACGAG TGGAAAATGC CCAAAAAGTC CTCCCTGGGT 
AGTCTGCCAT TCTCATCCGA TTTTGAGGAT TTCGACTACA GCTCGTGGGA TGCGATGTGC 
TATCTGGATC CGAGCAAAGC TGTTGAAGAG GATGACTTTG TGGTGGGGTT CTGGAATCCA 
TCAGAAGAAA ACTGTGGCGT CGACACAGGG AAGCAGTCCA TCTCTTACGA CCTGCACACC 
GAGCAGTGCA TTGCCGACAA GAGCATAGCT GACTGCGTGG AAGCCCTGCT GGGCTGCTAT 
TTAACCAGCT GTGGCGAGCG GGCTGCTCAG CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG 
CTCCCGGTAA TTAAAAGGAC TGATCGGGAA AAGGCCATGT GCCCGACCAG GGAGAATTTC 
ACCAGCCAAC AAAAGAACCT TTCAGGGAGC CGTATGGCCG CCTCTGGAGC CGGCTACCGA 
GCCTCTGTGT TGAAAGACTT GGAGTATGGG TGTTTGAAGA TCCCCCCGAG GTGTATGTTT 
GATCACCCCG ATGCAGACAG GACGCTGCGT CACCTGATCT CGGGTTTTGA AAATTTCGAA 
AAGAAAATCA ACTACAGATT CAAGAATAAG GCTTACCTTC TACAGGCTTT TACACACGCC 
TCCTACCACT ACAATACGAT CACGGATTGT TACCAGCGCT TAGAATTCCT GGGAGATGCA 
ATTTTGGACT ACCTCATAAC CAAGCACCTT TATGAAGACC CACGGCAGCA CTCCCCAGGG 
GTCCTGACTG ACCTGCGCTC TGCTCTGGTC AACAACACCA TCTTCGCGTC GTTGGCTGTG 
AAATATGACT ACCACAAGTA CTTTAAGGCC GTTTCTCCCG AGCTCTTCCA CGTCATCGAC 
GATTTCGTGC AGTTTCAGCT CGAGAAGAAC GAAATGCAAG GAATGGATTC CGAGCTTAGG 
AGATCCGAGG AGGATGAAGA GAAAGAAGAG GATATTGAAG TTCCAAAGGC CATGGGGGAT 
ATTTTCGAGT CACTTGCGGG CGCCATTTAC ATGGACAGCG GGATGTCGCT GGAGACGGTC 
TGGCAGGTGT ACTATCCCAT GATGCGGCCG CTGATCGAAA AATTTTCTGC AAATGTGCCC 
CGTTCCCCTG TGCGAGAATT GCTTGAAATG GAACCAGAAA CTGCCAAATT CAGCCCAGCT 
GAGAGAACCT ACGATGGCAA GGTCAGAGTC ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC 
AAAGGCGTTG GCCGAAGTTA CAGGATTGCC AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA 
AGCCTCAAAG CTAATCAACC TCAGGTTCCC AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014944191.1
CDS	2671..8349
Translation	MSKCSSDNSSTEISNIKTVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRSGRRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVTASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILRSNAETATDLVVVDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCERLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPADADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISVANSSSAENENYCKHSTIVVPENAAHQGANRTSSLENHDQMSVNCRTLFSESPGKLQVEVATDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTEAGEAPPDRTASEQSPSPGHSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAPKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRMAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_015088705.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon dicer 1, ribonuclease type III (DICER1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015088705.1

ORF Insert Sequence:

ATGTCAAAAT GCAGTTCAGA CAACAGCAGC ACAGAGATCT CAAACATTAA GACGGTTGAA 
CTGCTTGAAG CAGCTCTGGA TCATAATACC ATAGTCTGTT TAAACACTGG CTCAGGGAAG 
ACGTTTATTG CAGTGCTGCT CACTAAAGAG CTGTCTTACC AGATCAGGGG AGACTTCAAC 
AGAAGTGGCA GGAGGACGGT GTTCTTGGTC AACTCTGCAA ACCAGGTTGC TCAACAAGTG 
TCAGCTGTCA GAACTCACTC AGATCTCAAG GTCGGGGAAT ACTCAAACCT AGAAGTAACT 
GCATCTTGGA CAAAAGAGAA ATGGAACCAA GAGTTTACTA AACATCAGGT TCTCGTTATG 
ACTTGCTATG TCGCCTTGAA TGTTTTGAAA AATGGTTACT TATCACTGTC AGACATTAAC 
CTTTTGGTGT TTGATGAGTG TCATCTTGCA ATCCTAGACC ACCCCTACCG AGAAATTATG 
AAGCTTTGTG AAAATTGTCC GTCATGTCCT CGTATTTTGG GACTAACTGC TTCCATTTTA 
AATGGGAAAT GTGATCCAGA GGAATTGGAA GAAAAGATTC AGAAACTGGA GAAAATTCTT 
AGGAGTAATG CTGAAACTGC AACTGACTTG GTGGTCGTAG ACAGATATAC TTCTCAGCCA 
TGTGAGATTG TGGTAGACTG TGGACCATTT ACTGACAGAA GTGGGCTTTA TGAAAGACTG 
CTGATGGAGT TAGAAGAAGC ACTTAATTTT ATCAATGACT GTAACATATC TGTACATTCA 
AAAGAAAGAG ATTCTACTTT AATTTCTAAA CAGATACTCT CAGACTGCCG TGCGGTCCTA 
GTTGTCCTGG GACCCTGGTG TGCCGATAAA GTAGCTGGAA TGATGGTCAG AGAGCTACAG 
AAACACATCA AACACGAGCA AGAGGAGCTG CACCGGAAGT TTCTGTTGTT CACAGACACT 
TTCCTAAGGA AAATCCACGC CCTGTGTGAA GAGCACTTCT CACCTGCCTC CCTTGACCTG 
AAGTTTGTCA CTCCTAAAGT AATAAAGCTG CTCGAGATCT TACGCAAGTA CAAGCCGTAC 
GAGCGGCAGC AGTTTGAAAG CGTGGAATGG TATAATAATA GGAACCAGGA TAATTACGTG 
TCCTGGAGTG ATTCTGAGGA TGACGAGGAA GATGAAGAGA TTGAAGAGAA AGAGAAGCCA 
GAGACAAATT TTCCTTCTCC ATTTACCAAT ATTTTGTGTG GAATTATTTT TGTGGAAAGA 
AGATACACAG CGGTGGTCTT AAACAGATTG ATAAAGGAAG CTGGCAAACA AGATCCAGAG 
CTGGCTTACA TCAGCAGCAA TTTTATAACT GGACATGGCA TTGGAAAGAA TCAGCCTCGG 
AACAAACAGA TGGAAGCGGA ATTCAGAAAA CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA 
CATGAAACCA ACCTGCTTAT TGCAACAAGT ATTGTGGAAG AGGGTGTTGA CATACCGAAA 
TGCAACTTGG TGGTTCGTTT TGATCTGCCC ACAGAGTATC GATCCTATGT TCAGTCTAAG 
GGAAGAGCGA GGGCACCCAT CTCTAATTAT GTAATGTTAG CAGATACAGA TAAAATAAAG 
AGTTTTGAAG AAGACCTTAA AACATACAAA GCTATTGAAA AGATCTTGCG AAACAAATGT 
TCCAAGTCAG TTGATACCGG GGAGGCCGAC ACGGAGCCCG TGGTGGACGA TGACGATGTG 
TTCCCACCAT ACGTGCTGAG GCCTGAGGAC GGTCCTCGCG TCACGATCAA CACGGCCATT 
GGGCACGTCA ACAGATACTG TGCTAGATTA CCAAGTGATC CGTTTACTCA TCTGGCTCCT 
AAATGTAGAA CCCGAGAGTT GCCTGATGGT ACATTTTATT CAACTCTTTA TCTGCCAATT 
AACTCACCTC TTCGAGCCTC CATTGTTGGT CCCCCAATGA GCTGCATTCG ATTGGCTGAA 
AGAGTCGTGG CTCTCATTTG CTGTGAAAGA CTGCACAAAA TTGGTGAACT GGATGACCAT 
TTGATGCCCG TTGGGAAGGA GACTGTTAAA TATGAGGAGG AGCTGGATCT GCACGATGAA 
GAAGAGACTA GTGTTCCAGG AAGACCGGGC TCCACGAAGC GAAGGCAGTG CTACCCGAAA 
GCAATCCCCG AATGTTTGAG GGAGAGCTAC CCCAAGCCCG GTCAGCCCTG TTACCTGTAC 
GTGATCGGCA TGGTCCTGAC GACGCCTTTA CCTGATGAGC TCAACTTCAG AAGGCGGAAG 
CTCTACCCCC CCGAGGACAC CACGAGATGC TTTGGAATAC TGACAGCCAA GCCCATACCT 
CAGATCCCAC ACTTTCCTGT GTACACGCGC TCTGGAGAGG TGACCATATC TATTGAGTTG 
AAGAAGTCCG GCTTCACGTT ATCTCTGCAA ATGCTTGAGT TGATTACAAG ACTTCATCAG 
TATATATTCT CGCATATTCT TCGGCTTGAA AAACCTGCCC TAGAATTTAA ACCCGCAGAC 
GCGGACTCAG CCTACTGTGT TCTACCTCTT AACGTTGTTA ATGACTCCAG CACTTTGGAC 
ATTGACTTTA AATTCATGGA AGATATCGAG AAATCTGAAG CTCGCATAGG CATTCCCAGC 
ACAAAGTATT CAAAAGAAAC TCCCTTTGTT TTCAAATTAG AAGATTACCA AGATGCAGTT 
ATCATTCCAA GATACCGCAA TTTTGATCAG CCTCATCGAT TTTATGTCGC TGACGTGTAC 
ACTGATCTTA CCCCCCTGAG TAAATTTCCT TCCCCTGAGT ATGAAACGTT TGCGGAGTAT 
TATAAAACAA AGTATAACCT TGACCTGACC AATCTCAACC AGCCGCTGCT GGACGTGGAC 
CACACGTCTT CACGACTTAA TCTTTTGACA CCTCGCCATT TGAATCAGAA GGGGAAAGCT 
CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA GCCAAGTGGG AAAGCCTGCA GAATAAACAG 
ATACTGGTTC CAGAACTCTG TGCTATACAT CCAATTCCAG CATCACTGTG GAGAAAAGCC 
GTCTGTCTCC CCAGCATACT CTACCGCCTT CACTGCCTTC TGACTGCAGA GGAGCTCAGA 
GCCCAGACAG CCAGCGATGC TGGTGTGGGA GTCAGATCAC TTCCTGTGGA TTTTAGATAC 
CCTAACTTAG ACTTCGGGTG GAAAAAATCT ATCGACAGCA AATCTTTCAT CTCAGTTGCT 
AACTCCTCTT CAGCAGAAAA TGAGAATTAC TGTAAGCATA GCACAATTGT CGTCCCTGAA 
AATGCTGCAC ATCAAGGTGC TAATAGAACC TCCTCTCTAG AAAATCATGA CCAAATGTCT 
GTGAACTGCA GAACGCTGTT CAGCGAGTCA CCTGGTAAGC TCCAAGTTGA AGTTGCAACA 
GATCTGACAG CAATTAATGG TCTTTCTTAC AATAAAAGTC TTGCCAATGG CAGTTACGAT 
TTAGCTAACA GAGACTTTTG CCAAGGAAAC CATCTGAATT ACTACAAGCA GGAAATACCT 
GTACAACCAA CTACCTCATA TCCCATTCAG AATTTATACA ATTACGAGAA CCAGCCCAAG 
CCCAGCGATG AATGTACTCT ACTGAGTAAT AAATACCTTG ATGGAAATGC TAACACATCT 
ACCTCAGATG GAAGTCCCGT GACGGCCGCC GTGCCTGGTA CTACAGAGGC TGGGGAGGCG 
CCCCCGGACA GGACGGCTTC TGAGCAGAGC CCTTCTCCTG GGCACTCCTC GAGGACGCTC 
GGCCCCAACC CTGGGCTCAT TCTTCAGGCT CTGACCCTTT CGAATGCCAG CGACGGATTT 
AACCTGGAGC GGCTCGAGAT GCTTGGCGAC TCCTTCCTGA AGCACGCCAT CACCACGTAT 
CTCTTCTGCA CGTACCCCGA CGCGCACGAG GGCCGCCTTT CCTACATGAG AAGCAAAAAG 
GTCAGCAACT GTAATCTGTA TCGGCTTGGA AAAAAGAAGG GGCTGCCCAG CCGCATGGTG 
GTGTCCATAT TTGACCCCCC TGTGAATTGG CTTCCTCCTG GTTACGTAGT AAATCAAGAC 
AAAAGTAACA CAGAGAAATG GGAAAAAGAT GAAATGACAA AAGACTGCAT GTTGGCTAAT 
GGCAAGCTGG ACGATGACTT TGAGGAGGAG GAAGAGGAGG AGGAGGACCT GATGTGGAGG 
GCTCCCAAGG AGGACGCCGA CGATGAGGAC GACTTCCTGG AGTACGATCA GGAGCACATC 
AAGTTCATAG ATAACATGCT GATGGGGTCA GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT 
CCTTTTTCCG CCACCGATTC TGCATACGAG TGGAAAATGC CCAAAAAGTC CTCCCTGGGT 
AGTCTGCCAT TCTCATCCGA TTTTGAGGAT TTCGACTACA GCTCGTGGGA TGCGATGTGC 
TATCTGGATC CGAGCAAAGC TGTTGAAGAG GATGACTTTG TGGTGGGGTT CTGGAATCCA 
TCAGAAGAAA ACTGTGGCGT CGACACAGGG AAGCAGTCCA TCTCTTACGA CCTGCACACC 
GAGCAGTGCA TTGCCGACAA GAGCATAGCT GACTGCGTGG AAGCCCTGCT GGGCTGCTAT 
TTAACCAGCT GTGGCGAGCG GGCTGCTCAG CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG 
CTCCCGGTAA TTAAAAGGAC TGATCGGGAA AAGGCCATGT GCCCGACCAG GGAGAATTTC 
ACCAGCCAAC AAAAGAACCT TTCAGGGAGC CGTATGGCCG CCTCTGGAGC CGGCTACCGA 
GCCTCTGTGT TGAAAGACTT GGAGTATGGG TGTTTGAAGA TCCCCCCGAG GTGTATGTTT 
GATCACCCCG ATGCAGACAG GACGCTGCGT CACCTGATCT CGGGTTTTGA AAATTTCGAA 
AAGAAAATCA ACTACAGATT CAAGAATAAG GCTTACCTTC TACAGGCTTT TACACACGCC 
TCCTACCACT ACAATACGAT CACGGATTGT TACCAGCGCT TAGAATTCCT GGGAGATGCA 
ATTTTGGACT ACCTCATAAC CAAGCACCTT TATGAAGACC CACGGCAGCA CTCCCCAGGG 
GTCCTGACTG ACCTGCGCTC TGCTCTGGTC AACAACACCA TCTTCGCGTC GTTGGCTGTG 
AAATATGACT ACCACAAGTA CTTTAAGGCC GTTTCTCCCG AGCTCTTCCA CGTCATCGAC 
GATTTCGTGC AGTTTCAGCT CGAGAAGAAC GAAATGCAAG GAATGGATTC CGAGCTTAGG 
AGATCCGAGG AGGATGAAGA GAAAGAAGAG GATATTGAAG TTCCAAAGGC CATGGGGGAT 
ATTTTCGAGT CACTTGCGGG CGCCATTTAC ATGGACAGCG GGATGTCGCT GGAGACGGTC 
TGGCAGGTGT ACTATCCCAT GATGCGGCCG CTGATCGAAA AATTTTCTGC AAATGTGCCC 
CGTTCCCCTG TGCGAGAATT GCTTGAAATG GAACCAGAAA CTGCCAAATT CAGCCCAGCT 
GAGAGAACCT ACGATGGCAA GGTCAGAGTC ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC 
AAAGGCGTTG GCCGAAGTTA CAGGATTGCC AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA 
AGCCTCAAAG CTAATCAACC TCAGGTTCCC AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_015088703.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014944189.1 Ovis aries musimon dicer 1, ribonuclease type III (DICER1), transcript variant X11, mRNA.

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CloneID	OOv02801
Clone ID Related Accession (Same CDS sequence)	XM_012112873.2 , XM_015088705.1 , XM_015088703.1
Accession Version	XM_015088703.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	endoribonuclease Dicer isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942251.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAAT GCAGTTCAGA CAACAGCAGC ACAGAGATCT CAAACATTAA GACGGTTGAA 
CTGCTTGAAG CAGCTCTGGA TCATAATACC ATAGTCTGTT TAAACACTGG CTCAGGGAAG 
ACGTTTATTG CAGTGCTGCT CACTAAAGAG CTGTCTTACC AGATCAGGGG AGACTTCAAC 
AGAAGTGGCA GGAGGACGGT GTTCTTGGTC AACTCTGCAA ACCAGGTTGC TCAACAAGTG 
TCAGCTGTCA GAACTCACTC AGATCTCAAG GTCGGGGAAT ACTCAAACCT AGAAGTAACT 
GCATCTTGGA CAAAAGAGAA ATGGAACCAA GAGTTTACTA AACATCAGGT TCTCGTTATG 
ACTTGCTATG TCGCCTTGAA TGTTTTGAAA AATGGTTACT TATCACTGTC AGACATTAAC 
CTTTTGGTGT TTGATGAGTG TCATCTTGCA ATCCTAGACC ACCCCTACCG AGAAATTATG 
AAGCTTTGTG AAAATTGTCC GTCATGTCCT CGTATTTTGG GACTAACTGC TTCCATTTTA 
AATGGGAAAT GTGATCCAGA GGAATTGGAA GAAAAGATTC AGAAACTGGA GAAAATTCTT 
AGGAGTAATG CTGAAACTGC AACTGACTTG GTGGTCGTAG ACAGATATAC TTCTCAGCCA 
TGTGAGATTG TGGTAGACTG TGGACCATTT ACTGACAGAA GTGGGCTTTA TGAAAGACTG 
CTGATGGAGT TAGAAGAAGC ACTTAATTTT ATCAATGACT GTAACATATC TGTACATTCA 
AAAGAAAGAG ATTCTACTTT AATTTCTAAA CAGATACTCT CAGACTGCCG TGCGGTCCTA 
GTTGTCCTGG GACCCTGGTG TGCCGATAAA GTAGCTGGAA TGATGGTCAG AGAGCTACAG 
AAACACATCA AACACGAGCA AGAGGAGCTG CACCGGAAGT TTCTGTTGTT CACAGACACT 
TTCCTAAGGA AAATCCACGC CCTGTGTGAA GAGCACTTCT CACCTGCCTC CCTTGACCTG 
AAGTTTGTCA CTCCTAAAGT AATAAAGCTG CTCGAGATCT TACGCAAGTA CAAGCCGTAC 
GAGCGGCAGC AGTTTGAAAG CGTGGAATGG TATAATAATA GGAACCAGGA TAATTACGTG 
TCCTGGAGTG ATTCTGAGGA TGACGAGGAA GATGAAGAGA TTGAAGAGAA AGAGAAGCCA 
GAGACAAATT TTCCTTCTCC ATTTACCAAT ATTTTGTGTG GAATTATTTT TGTGGAAAGA 
AGATACACAG CGGTGGTCTT AAACAGATTG ATAAAGGAAG CTGGCAAACA AGATCCAGAG 
CTGGCTTACA TCAGCAGCAA TTTTATAACT GGACATGGCA TTGGAAAGAA TCAGCCTCGG 
AACAAACAGA TGGAAGCGGA ATTCAGAAAA CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA 
CATGAAACCA ACCTGCTTAT TGCAACAAGT ATTGTGGAAG AGGGTGTTGA CATACCGAAA 
TGCAACTTGG TGGTTCGTTT TGATCTGCCC ACAGAGTATC GATCCTATGT TCAGTCTAAG 
GGAAGAGCGA GGGCACCCAT CTCTAATTAT GTAATGTTAG CAGATACAGA TAAAATAAAG 
AGTTTTGAAG AAGACCTTAA AACATACAAA GCTATTGAAA AGATCTTGCG AAACAAATGT 
TCCAAGTCAG TTGATACCGG GGAGGCCGAC ACGGAGCCCG TGGTGGACGA TGACGATGTG 
TTCCCACCAT ACGTGCTGAG GCCTGAGGAC GGTCCTCGCG TCACGATCAA CACGGCCATT 
GGGCACGTCA ACAGATACTG TGCTAGATTA CCAAGTGATC CGTTTACTCA TCTGGCTCCT 
AAATGTAGAA CCCGAGAGTT GCCTGATGGT ACATTTTATT CAACTCTTTA TCTGCCAATT 
AACTCACCTC TTCGAGCCTC CATTGTTGGT CCCCCAATGA GCTGCATTCG ATTGGCTGAA 
AGAGTCGTGG CTCTCATTTG CTGTGAAAGA CTGCACAAAA TTGGTGAACT GGATGACCAT 
TTGATGCCCG TTGGGAAGGA GACTGTTAAA TATGAGGAGG AGCTGGATCT GCACGATGAA 
GAAGAGACTA GTGTTCCAGG AAGACCGGGC TCCACGAAGC GAAGGCAGTG CTACCCGAAA 
GCAATCCCCG AATGTTTGAG GGAGAGCTAC CCCAAGCCCG GTCAGCCCTG TTACCTGTAC 
GTGATCGGCA TGGTCCTGAC GACGCCTTTA CCTGATGAGC TCAACTTCAG AAGGCGGAAG 
CTCTACCCCC CCGAGGACAC CACGAGATGC TTTGGAATAC TGACAGCCAA GCCCATACCT 
CAGATCCCAC ACTTTCCTGT GTACACGCGC TCTGGAGAGG TGACCATATC TATTGAGTTG 
AAGAAGTCCG GCTTCACGTT ATCTCTGCAA ATGCTTGAGT TGATTACAAG ACTTCATCAG 
TATATATTCT CGCATATTCT TCGGCTTGAA AAACCTGCCC TAGAATTTAA ACCCGCAGAC 
GCGGACTCAG CCTACTGTGT TCTACCTCTT AACGTTGTTA ATGACTCCAG CACTTTGGAC 
ATTGACTTTA AATTCATGGA AGATATCGAG AAATCTGAAG CTCGCATAGG CATTCCCAGC 
ACAAAGTATT CAAAAGAAAC TCCCTTTGTT TTCAAATTAG AAGATTACCA AGATGCAGTT 
ATCATTCCAA GATACCGCAA TTTTGATCAG CCTCATCGAT TTTATGTCGC TGACGTGTAC 
ACTGATCTTA CCCCCCTGAG TAAATTTCCT TCCCCTGAGT ATGAAACGTT TGCGGAGTAT 
TATAAAACAA AGTATAACCT TGACCTGACC AATCTCAACC AGCCGCTGCT GGACGTGGAC 
CACACGTCTT CACGACTTAA TCTTTTGACA CCTCGCCATT TGAATCAGAA GGGGAAAGCT 
CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA GCCAAGTGGG AAAGCCTGCA GAATAAACAG 
ATACTGGTTC CAGAACTCTG TGCTATACAT CCAATTCCAG CATCACTGTG GAGAAAAGCC 
GTCTGTCTCC CCAGCATACT CTACCGCCTT CACTGCCTTC TGACTGCAGA GGAGCTCAGA 
GCCCAGACAG CCAGCGATGC TGGTGTGGGA GTCAGATCAC TTCCTGTGGA TTTTAGATAC 
CCTAACTTAG ACTTCGGGTG GAAAAAATCT ATCGACAGCA AATCTTTCAT CTCAGTTGCT 
AACTCCTCTT CAGCAGAAAA TGAGAATTAC TGTAAGCATA GCACAATTGT CGTCCCTGAA 
AATGCTGCAC ATCAAGGTGC TAATAGAACC TCCTCTCTAG AAAATCATGA CCAAATGTCT 
GTGAACTGCA GAACGCTGTT CAGCGAGTCA CCTGGTAAGC TCCAAGTTGA AGTTGCAACA 
GATCTGACAG CAATTAATGG TCTTTCTTAC AATAAAAGTC TTGCCAATGG CAGTTACGAT 
TTAGCTAACA GAGACTTTTG CCAAGGAAAC CATCTGAATT ACTACAAGCA GGAAATACCT 
GTACAACCAA CTACCTCATA TCCCATTCAG AATTTATACA ATTACGAGAA CCAGCCCAAG 
CCCAGCGATG AATGTACTCT ACTGAGTAAT AAATACCTTG ATGGAAATGC TAACACATCT 
ACCTCAGATG GAAGTCCCGT GACGGCCGCC GTGCCTGGTA CTACAGAGGC TGGGGAGGCG 
CCCCCGGACA GGACGGCTTC TGAGCAGAGC CCTTCTCCTG GGCACTCCTC GAGGACGCTC 
GGCCCCAACC CTGGGCTCAT TCTTCAGGCT CTGACCCTTT CGAATGCCAG CGACGGATTT 
AACCTGGAGC GGCTCGAGAT GCTTGGCGAC TCCTTCCTGA AGCACGCCAT CACCACGTAT 
CTCTTCTGCA CGTACCCCGA CGCGCACGAG GGCCGCCTTT CCTACATGAG AAGCAAAAAG 
GTCAGCAACT GTAATCTGTA TCGGCTTGGA AAAAAGAAGG GGCTGCCCAG CCGCATGGTG 
GTGTCCATAT TTGACCCCCC TGTGAATTGG CTTCCTCCTG GTTACGTAGT AAATCAAGAC 
AAAAGTAACA CAGAGAAATG GGAAAAAGAT GAAATGACAA AAGACTGCAT GTTGGCTAAT 
GGCAAGCTGG ACGATGACTT TGAGGAGGAG GAAGAGGAGG AGGAGGACCT GATGTGGAGG 
GCTCCCAAGG AGGACGCCGA CGATGAGGAC GACTTCCTGG AGTACGATCA GGAGCACATC 
AAGTTCATAG ATAACATGCT GATGGGGTCA GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT 
CCTTTTTCCG CCACCGATTC TGCATACGAG TGGAAAATGC CCAAAAAGTC CTCCCTGGGT 
AGTCTGCCAT TCTCATCCGA TTTTGAGGAT TTCGACTACA GCTCGTGGGA TGCGATGTGC 
TATCTGGATC CGAGCAAAGC TGTTGAAGAG GATGACTTTG TGGTGGGGTT CTGGAATCCA 
TCAGAAGAAA ACTGTGGCGT CGACACAGGG AAGCAGTCCA TCTCTTACGA CCTGCACACC 
GAGCAGTGCA TTGCCGACAA GAGCATAGCT GACTGCGTGG AAGCCCTGCT GGGCTGCTAT 
TTAACCAGCT GTGGCGAGCG GGCTGCTCAG CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG 
CTCCCGGTAA TTAAAAGGAC TGATCGGGAA AAGGCCATGT GCCCGACCAG GGAGAATTTC 
ACCAGCCAAC AAAAGAACCT TTCAGGGAGC CGTATGGCCG CCTCTGGAGC CGGCTACCGA 
GCCTCTGTGT TGAAAGACTT GGAGTATGGG TGTTTGAAGA TCCCCCCGAG GTGTATGTTT 
GATCACCCCG ATGCAGACAG GACGCTGCGT CACCTGATCT CGGGTTTTGA AAATTTCGAA 
AAGAAAATCA ACTACAGATT CAAGAATAAG GCTTACCTTC TACAGGCTTT TACACACGCC 
TCCTACCACT ACAATACGAT CACGGATTGT TACCAGCGCT TAGAATTCCT GGGAGATGCA 
ATTTTGGACT ACCTCATAAC CAAGCACCTT TATGAAGACC CACGGCAGCA CTCCCCAGGG 
GTCCTGACTG ACCTGCGCTC TGCTCTGGTC AACAACACCA TCTTCGCGTC GTTGGCTGTG 
AAATATGACT ACCACAAGTA CTTTAAGGCC GTTTCTCCCG AGCTCTTCCA CGTCATCGAC 
GATTTCGTGC AGTTTCAGCT CGAGAAGAAC GAAATGCAAG GAATGGATTC CGAGCTTAGG 
AGATCCGAGG AGGATGAAGA GAAAGAAGAG GATATTGAAG TTCCAAAGGC CATGGGGGAT 
ATTTTCGAGT CACTTGCGGG CGCCATTTAC ATGGACAGCG GGATGTCGCT GGAGACGGTC 
TGGCAGGTGT ACTATCCCAT GATGCGGCCG CTGATCGAAA AATTTTCTGC AAATGTGCCC 
CGTTCCCCTG TGCGAGAATT GCTTGAAATG GAACCAGAAA CTGCCAAATT CAGCCCAGCT 
GAGAGAACCT ACGATGGCAA GGTCAGAGTC ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC 
AAAGGCGTTG GCCGAAGTTA CAGGATTGCC AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA 
AGCCTCAAAG CTAATCAACC TCAGGTTCCC AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014944189.1
CDS	3710..9388
Translation	MSKCSSDNSSTEISNIKTVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRSGRRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVTASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILRSNAETATDLVVVDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCERLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPADADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISVANSSSAENENYCKHSTIVVPENAAHQGANRTSSLENHDQMSVNCRTLFSESPGKLQVEVATDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTEAGEAPPDRTASEQSPSPGHSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAPKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRMAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_015088703.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon dicer 1, ribonuclease type III (DICER1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015088703.1

ORF Insert Sequence:

ATGTCAAAAT GCAGTTCAGA CAACAGCAGC ACAGAGATCT CAAACATTAA GACGGTTGAA 
CTGCTTGAAG CAGCTCTGGA TCATAATACC ATAGTCTGTT TAAACACTGG CTCAGGGAAG 
ACGTTTATTG CAGTGCTGCT CACTAAAGAG CTGTCTTACC AGATCAGGGG AGACTTCAAC 
AGAAGTGGCA GGAGGACGGT GTTCTTGGTC AACTCTGCAA ACCAGGTTGC TCAACAAGTG 
TCAGCTGTCA GAACTCACTC AGATCTCAAG GTCGGGGAAT ACTCAAACCT AGAAGTAACT 
GCATCTTGGA CAAAAGAGAA ATGGAACCAA GAGTTTACTA AACATCAGGT TCTCGTTATG 
ACTTGCTATG TCGCCTTGAA TGTTTTGAAA AATGGTTACT TATCACTGTC AGACATTAAC 
CTTTTGGTGT TTGATGAGTG TCATCTTGCA ATCCTAGACC ACCCCTACCG AGAAATTATG 
AAGCTTTGTG AAAATTGTCC GTCATGTCCT CGTATTTTGG GACTAACTGC TTCCATTTTA 
AATGGGAAAT GTGATCCAGA GGAATTGGAA GAAAAGATTC AGAAACTGGA GAAAATTCTT 
AGGAGTAATG CTGAAACTGC AACTGACTTG GTGGTCGTAG ACAGATATAC TTCTCAGCCA 
TGTGAGATTG TGGTAGACTG TGGACCATTT ACTGACAGAA GTGGGCTTTA TGAAAGACTG 
CTGATGGAGT TAGAAGAAGC ACTTAATTTT ATCAATGACT GTAACATATC TGTACATTCA 
AAAGAAAGAG ATTCTACTTT AATTTCTAAA CAGATACTCT CAGACTGCCG TGCGGTCCTA 
GTTGTCCTGG GACCCTGGTG TGCCGATAAA GTAGCTGGAA TGATGGTCAG AGAGCTACAG 
AAACACATCA AACACGAGCA AGAGGAGCTG CACCGGAAGT TTCTGTTGTT CACAGACACT 
TTCCTAAGGA AAATCCACGC CCTGTGTGAA GAGCACTTCT CACCTGCCTC CCTTGACCTG 
AAGTTTGTCA CTCCTAAAGT AATAAAGCTG CTCGAGATCT TACGCAAGTA CAAGCCGTAC 
GAGCGGCAGC AGTTTGAAAG CGTGGAATGG TATAATAATA GGAACCAGGA TAATTACGTG 
TCCTGGAGTG ATTCTGAGGA TGACGAGGAA GATGAAGAGA TTGAAGAGAA AGAGAAGCCA 
GAGACAAATT TTCCTTCTCC ATTTACCAAT ATTTTGTGTG GAATTATTTT TGTGGAAAGA 
AGATACACAG CGGTGGTCTT AAACAGATTG ATAAAGGAAG CTGGCAAACA AGATCCAGAG 
CTGGCTTACA TCAGCAGCAA TTTTATAACT GGACATGGCA TTGGAAAGAA TCAGCCTCGG 
AACAAACAGA TGGAAGCGGA ATTCAGAAAA CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA 
CATGAAACCA ACCTGCTTAT TGCAACAAGT ATTGTGGAAG AGGGTGTTGA CATACCGAAA 
TGCAACTTGG TGGTTCGTTT TGATCTGCCC ACAGAGTATC GATCCTATGT TCAGTCTAAG 
GGAAGAGCGA GGGCACCCAT CTCTAATTAT GTAATGTTAG CAGATACAGA TAAAATAAAG 
AGTTTTGAAG AAGACCTTAA AACATACAAA GCTATTGAAA AGATCTTGCG AAACAAATGT 
TCCAAGTCAG TTGATACCGG GGAGGCCGAC ACGGAGCCCG TGGTGGACGA TGACGATGTG 
TTCCCACCAT ACGTGCTGAG GCCTGAGGAC GGTCCTCGCG TCACGATCAA CACGGCCATT 
GGGCACGTCA ACAGATACTG TGCTAGATTA CCAAGTGATC CGTTTACTCA TCTGGCTCCT 
AAATGTAGAA CCCGAGAGTT GCCTGATGGT ACATTTTATT CAACTCTTTA TCTGCCAATT 
AACTCACCTC TTCGAGCCTC CATTGTTGGT CCCCCAATGA GCTGCATTCG ATTGGCTGAA 
AGAGTCGTGG CTCTCATTTG CTGTGAAAGA CTGCACAAAA TTGGTGAACT GGATGACCAT 
TTGATGCCCG TTGGGAAGGA GACTGTTAAA TATGAGGAGG AGCTGGATCT GCACGATGAA 
GAAGAGACTA GTGTTCCAGG AAGACCGGGC TCCACGAAGC GAAGGCAGTG CTACCCGAAA 
GCAATCCCCG AATGTTTGAG GGAGAGCTAC CCCAAGCCCG GTCAGCCCTG TTACCTGTAC 
GTGATCGGCA TGGTCCTGAC GACGCCTTTA CCTGATGAGC TCAACTTCAG AAGGCGGAAG 
CTCTACCCCC CCGAGGACAC CACGAGATGC TTTGGAATAC TGACAGCCAA GCCCATACCT 
CAGATCCCAC ACTTTCCTGT GTACACGCGC TCTGGAGAGG TGACCATATC TATTGAGTTG 
AAGAAGTCCG GCTTCACGTT ATCTCTGCAA ATGCTTGAGT TGATTACAAG ACTTCATCAG 
TATATATTCT CGCATATTCT TCGGCTTGAA AAACCTGCCC TAGAATTTAA ACCCGCAGAC 
GCGGACTCAG CCTACTGTGT TCTACCTCTT AACGTTGTTA ATGACTCCAG CACTTTGGAC 
ATTGACTTTA AATTCATGGA AGATATCGAG AAATCTGAAG CTCGCATAGG CATTCCCAGC 
ACAAAGTATT CAAAAGAAAC TCCCTTTGTT TTCAAATTAG AAGATTACCA AGATGCAGTT 
ATCATTCCAA GATACCGCAA TTTTGATCAG CCTCATCGAT TTTATGTCGC TGACGTGTAC 
ACTGATCTTA CCCCCCTGAG TAAATTTCCT TCCCCTGAGT ATGAAACGTT TGCGGAGTAT 
TATAAAACAA AGTATAACCT TGACCTGACC AATCTCAACC AGCCGCTGCT GGACGTGGAC 
CACACGTCTT CACGACTTAA TCTTTTGACA CCTCGCCATT TGAATCAGAA GGGGAAAGCT 
CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA GCCAAGTGGG AAAGCCTGCA GAATAAACAG 
ATACTGGTTC CAGAACTCTG TGCTATACAT CCAATTCCAG CATCACTGTG GAGAAAAGCC 
GTCTGTCTCC CCAGCATACT CTACCGCCTT CACTGCCTTC TGACTGCAGA GGAGCTCAGA 
GCCCAGACAG CCAGCGATGC TGGTGTGGGA GTCAGATCAC TTCCTGTGGA TTTTAGATAC 
CCTAACTTAG ACTTCGGGTG GAAAAAATCT ATCGACAGCA AATCTTTCAT CTCAGTTGCT 
AACTCCTCTT CAGCAGAAAA TGAGAATTAC TGTAAGCATA GCACAATTGT CGTCCCTGAA 
AATGCTGCAC ATCAAGGTGC TAATAGAACC TCCTCTCTAG AAAATCATGA CCAAATGTCT 
GTGAACTGCA GAACGCTGTT CAGCGAGTCA CCTGGTAAGC TCCAAGTTGA AGTTGCAACA 
GATCTGACAG CAATTAATGG TCTTTCTTAC AATAAAAGTC TTGCCAATGG CAGTTACGAT 
TTAGCTAACA GAGACTTTTG CCAAGGAAAC CATCTGAATT ACTACAAGCA GGAAATACCT 
GTACAACCAA CTACCTCATA TCCCATTCAG AATTTATACA ATTACGAGAA CCAGCCCAAG 
CCCAGCGATG AATGTACTCT ACTGAGTAAT AAATACCTTG ATGGAAATGC TAACACATCT 
ACCTCAGATG GAAGTCCCGT GACGGCCGCC GTGCCTGGTA CTACAGAGGC TGGGGAGGCG 
CCCCCGGACA GGACGGCTTC TGAGCAGAGC CCTTCTCCTG GGCACTCCTC GAGGACGCTC 
GGCCCCAACC CTGGGCTCAT TCTTCAGGCT CTGACCCTTT CGAATGCCAG CGACGGATTT 
AACCTGGAGC GGCTCGAGAT GCTTGGCGAC TCCTTCCTGA AGCACGCCAT CACCACGTAT 
CTCTTCTGCA CGTACCCCGA CGCGCACGAG GGCCGCCTTT CCTACATGAG AAGCAAAAAG 
GTCAGCAACT GTAATCTGTA TCGGCTTGGA AAAAAGAAGG GGCTGCCCAG CCGCATGGTG 
GTGTCCATAT TTGACCCCCC TGTGAATTGG CTTCCTCCTG GTTACGTAGT AAATCAAGAC 
AAAAGTAACA CAGAGAAATG GGAAAAAGAT GAAATGACAA AAGACTGCAT GTTGGCTAAT 
GGCAAGCTGG ACGATGACTT TGAGGAGGAG GAAGAGGAGG AGGAGGACCT GATGTGGAGG 
GCTCCCAAGG AGGACGCCGA CGATGAGGAC GACTTCCTGG AGTACGATCA GGAGCACATC 
AAGTTCATAG ATAACATGCT GATGGGGTCA GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT 
CCTTTTTCCG CCACCGATTC TGCATACGAG TGGAAAATGC CCAAAAAGTC CTCCCTGGGT 
AGTCTGCCAT TCTCATCCGA TTTTGAGGAT TTCGACTACA GCTCGTGGGA TGCGATGTGC 
TATCTGGATC CGAGCAAAGC TGTTGAAGAG GATGACTTTG TGGTGGGGTT CTGGAATCCA 
TCAGAAGAAA ACTGTGGCGT CGACACAGGG AAGCAGTCCA TCTCTTACGA CCTGCACACC 
GAGCAGTGCA TTGCCGACAA GAGCATAGCT GACTGCGTGG AAGCCCTGCT GGGCTGCTAT 
TTAACCAGCT GTGGCGAGCG GGCTGCTCAG CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG 
CTCCCGGTAA TTAAAAGGAC TGATCGGGAA AAGGCCATGT GCCCGACCAG GGAGAATTTC 
ACCAGCCAAC AAAAGAACCT TTCAGGGAGC CGTATGGCCG CCTCTGGAGC CGGCTACCGA 
GCCTCTGTGT TGAAAGACTT GGAGTATGGG TGTTTGAAGA TCCCCCCGAG GTGTATGTTT 
GATCACCCCG ATGCAGACAG GACGCTGCGT CACCTGATCT CGGGTTTTGA AAATTTCGAA 
AAGAAAATCA ACTACAGATT CAAGAATAAG GCTTACCTTC TACAGGCTTT TACACACGCC 
TCCTACCACT ACAATACGAT CACGGATTGT TACCAGCGCT TAGAATTCCT GGGAGATGCA 
ATTTTGGACT ACCTCATAAC CAAGCACCTT TATGAAGACC CACGGCAGCA CTCCCCAGGG 
GTCCTGACTG ACCTGCGCTC TGCTCTGGTC AACAACACCA TCTTCGCGTC GTTGGCTGTG 
AAATATGACT ACCACAAGTA CTTTAAGGCC GTTTCTCCCG AGCTCTTCCA CGTCATCGAC 
GATTTCGTGC AGTTTCAGCT CGAGAAGAAC GAAATGCAAG GAATGGATTC CGAGCTTAGG 
AGATCCGAGG AGGATGAAGA GAAAGAAGAG GATATTGAAG TTCCAAAGGC CATGGGGGAT 
ATTTTCGAGT CACTTGCGGG CGCCATTTAC ATGGACAGCG GGATGTCGCT GGAGACGGTC 
TGGCAGGTGT ACTATCCCAT GATGCGGCCG CTGATCGAAA AATTTTCTGC AAATGTGCCC 
CGTTCCCCTG TGCGAGAATT GCTTGAAATG GAACCAGAAA CTGCCAAATT CAGCCCAGCT 
GAGAGAACCT ACGATGGCAA GGTCAGAGTC ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC 
AAAGGCGTTG GCCGAAGTTA CAGGATTGCC AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA 
AGCCTCAAAG CTAATCAACC TCAGGTTCCC AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_004017979.3 ) cDNA ORF clone, Ovis aries(sheep) -> XP_004018028.1 Ovis aries dicer 1, ribonuclease type III (DICER1), transcript variant X1, mRNA.

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CloneID	OSm14330
Clone ID Related Accession (Same CDS sequence)	XM_004017979.3 , XM_015102037.1 , XM_012099009.2 , XM_015102036.1
Accession Version	XM_004017979.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_004017979.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTGCTGCT CACTAAAGAG 
CTGTCTTACC AGATCAGGGG AGACTTCAAC AGAAGTGGCA GGAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAACT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC GTCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTT AGGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCGTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTA GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTACAG AAACACATCA AACACGAGCA AGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTAAGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CACCTGCCTC CCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAGTA CAAGCCGTAC GAGCGGCAGC AGTTTGAAAG CGTGGAATGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGTG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC ATTTACCAAT 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CGGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT TGATCTGCCC 
ACAGAGTATC GATCCTATGT TCAGTCTAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGATACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCAG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGACGA TGACGATGTG TTCCCACCAT ACGTGCTGAG GCCTGAGGAC 
GGTCCTCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG ATTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAGA 
CTGCACAAAA TTGGTGAACT GGATGACCAT TTGATGCCCG TTGGGAAGGA GACTGTTAAA 
TATGAGGAGG AGCTGGATCT GCACGATGAA GAAGAGACTA GTGTTCCAGG AAGACCGGGC 
TCCACGAAGC GAAGGCAGTG CTACCCGAAA GCAATCCCCG AATGTTTGAG GGAGAGCTAC 
CCCAAGCCCG GTCAGCCCTG TTACCTGTAC GTGATCGGCA TGGTCCTGAC GACGCCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CCGAGGACAC CACGAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATTACAAG ACTTCACCAG TATATATTCT CGCATATTCT TCGGCTTGAA 
AAACCTGCCC TAGAATTTAA ACCCGCAGAC GCGGACTCAG CCTACTGTGT TCTACCTCTT 
AACGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGCATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCCTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTTAA TCTTTTGACA 
CCTCGCCATT TGAATCAGAA GGGGAAAGCT CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AAAGCCTGCA GAATAAACAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTCTGTCTCC CCAGCATACT CTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACAG CCAGCGATGC TGGTGTGGGA 
GTCAGATCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAGTTGCT AACTCCTCTT CAGCAGAAAA TGAGAATTAC 
TGTAAGCATA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGCTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAGTTGA AGTTGCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCCGCC 
GTGCCTGGTA CTACAGAGGC TGGGGAGGCG CCCCCGGACA GGACGGCTTC TGAGCAGAGC 
CCTTCTCCTG GGCACTCCTC GAGGACGCTC GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
CTGACCCTTT CGAATGCCAG CGACGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTGA AGCACGCCAT CACCACGTAT CTCTTCTGCA CGTACCCCGA CGCGCACGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAGCTGG ACGATGACTT TGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCCCAAGG AGGACGCCGA CGATGAGGAC 
GACTTCCTGG AGTACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT CCTTTTTCCG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTGGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCGTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC TGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGCGT CGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA GAGCATAGCT 
GACTGCGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGCG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTACGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ATGCAGACAG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAACACCA TCTTCGCGTC GTTGGCTGTG AAATATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA CGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC CGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTTGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004018028.1
CDS	2768..8536
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRSGRRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVTASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILRSNAETATDLVVVDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCERLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPADADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISVANSSSAENENYCKHSTIVVPENAAHQGANRTSSLENHDQMSVNCRTLFSESPGKLQVEVATDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTEAGEAPPDRTASEQSPSPGHSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAPKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRTAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_004017979.3
Organism	Ovis aries(sheep)
Definition	Ovis aries dicer 1, ribonuclease type III (DICER1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004017979.3

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTGCTGCT CACTAAAGAG 
CTGTCTTACC AGATCAGGGG AGACTTCAAC AGAAGTGGCA GGAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAACT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC GTCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTT AGGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCGTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTA GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTACAG AAACACATCA AACACGAGCA AGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTAAGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CACCTGCCTC CCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAGTA CAAGCCGTAC GAGCGGCAGC AGTTTGAAAG CGTGGAATGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGTG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC ATTTACCAAT 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CGGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT TGATCTGCCC 
ACAGAGTATC GATCCTATGT TCAGTCTAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGATACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCAG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGACGA TGACGATGTG TTCCCACCAT ACGTGCTGAG GCCTGAGGAC 
GGTCCTCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG ATTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAGA 
CTGCACAAAA TTGGTGAACT GGATGACCAT TTGATGCCCG TTGGGAAGGA GACTGTTAAA 
TATGAGGAGG AGCTGGATCT GCACGATGAA GAAGAGACTA GTGTTCCAGG AAGACCGGGC 
TCCACGAAGC GAAGGCAGTG CTACCCGAAA GCAATCCCCG AATGTTTGAG GGAGAGCTAC 
CCCAAGCCCG GTCAGCCCTG TTACCTGTAC GTGATCGGCA TGGTCCTGAC GACGCCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CCGAGGACAC CACGAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATTACAAG ACTTCACCAG TATATATTCT CGCATATTCT TCGGCTTGAA 
AAACCTGCCC TAGAATTTAA ACCCGCAGAC GCGGACTCAG CCTACTGTGT TCTACCTCTT 
AACGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGCATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCCTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTTAA TCTTTTGACA 
CCTCGCCATT TGAATCAGAA GGGGAAAGCT CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AAAGCCTGCA GAATAAACAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTCTGTCTCC CCAGCATACT CTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACAG CCAGCGATGC TGGTGTGGGA 
GTCAGATCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAGTTGCT AACTCCTCTT CAGCAGAAAA TGAGAATTAC 
TGTAAGCATA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGCTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAGTTGA AGTTGCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCCGCC 
GTGCCTGGTA CTACAGAGGC TGGGGAGGCG CCCCCGGACA GGACGGCTTC TGAGCAGAGC 
CCTTCTCCTG GGCACTCCTC GAGGACGCTC GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
CTGACCCTTT CGAATGCCAG CGACGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTGA AGCACGCCAT CACCACGTAT CTCTTCTGCA CGTACCCCGA CGCGCACGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAGCTGG ACGATGACTT TGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCCCAAGG AGGACGCCGA CGATGAGGAC 
GACTTCCTGG AGTACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT CCTTTTTCCG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTGGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCGTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC TGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGCGT CGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA GAGCATAGCT 
GACTGCGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGCG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTACGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ATGCAGACAG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAACACCA TCTTCGCGTC GTTGGCTGTG AAATATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA CGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC CGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTTGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_015102037.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014957523.1 Ovis aries dicer 1, ribonuclease type III (DICER1), transcript variant X4, mRNA.

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CloneID	OSm14330
Clone ID Related Accession (Same CDS sequence)	XM_004017979.3 , XM_015102037.1 , XM_012099009.2 , XM_015102036.1
Accession Version	XM_015102037.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTGCTGCT CACTAAAGAG 
CTGTCTTACC AGATCAGGGG AGACTTCAAC AGAAGTGGCA GGAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAACT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC GTCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTT AGGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCGTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTA GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTACAG AAACACATCA AACACGAGCA AGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTAAGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CACCTGCCTC CCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAGTA CAAGCCGTAC GAGCGGCAGC AGTTTGAAAG CGTGGAATGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGTG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC ATTTACCAAT 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CGGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT TGATCTGCCC 
ACAGAGTATC GATCCTATGT TCAGTCTAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGATACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCAG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGACGA TGACGATGTG TTCCCACCAT ACGTGCTGAG GCCTGAGGAC 
GGTCCTCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG ATTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAGA 
CTGCACAAAA TTGGTGAACT GGATGACCAT TTGATGCCCG TTGGGAAGGA GACTGTTAAA 
TATGAGGAGG AGCTGGATCT GCACGATGAA GAAGAGACTA GTGTTCCAGG AAGACCGGGC 
TCCACGAAGC GAAGGCAGTG CTACCCGAAA GCAATCCCCG AATGTTTGAG GGAGAGCTAC 
CCCAAGCCCG GTCAGCCCTG TTACCTGTAC GTGATCGGCA TGGTCCTGAC GACGCCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CCGAGGACAC CACGAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATTACAAG ACTTCACCAG TATATATTCT CGCATATTCT TCGGCTTGAA 
AAACCTGCCC TAGAATTTAA ACCCGCAGAC GCGGACTCAG CCTACTGTGT TCTACCTCTT 
AACGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGCATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCCTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTTAA TCTTTTGACA 
CCTCGCCATT TGAATCAGAA GGGGAAAGCT CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AAAGCCTGCA GAATAAACAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTCTGTCTCC CCAGCATACT CTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACAG CCAGCGATGC TGGTGTGGGA 
GTCAGATCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAGTTGCT AACTCCTCTT CAGCAGAAAA TGAGAATTAC 
TGTAAGCATA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGCTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAGTTGA AGTTGCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCCGCC 
GTGCCTGGTA CTACAGAGGC TGGGGAGGCG CCCCCGGACA GGACGGCTTC TGAGCAGAGC 
CCTTCTCCTG GGCACTCCTC GAGGACGCTC GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
CTGACCCTTT CGAATGCCAG CGACGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTGA AGCACGCCAT CACCACGTAT CTCTTCTGCA CGTACCCCGA CGCGCACGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAGCTGG ACGATGACTT TGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCCCAAGG AGGACGCCGA CGATGAGGAC 
GACTTCCTGG AGTACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT CCTTTTTCCG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTGGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCGTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC TGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGCGT CGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA GAGCATAGCT 
GACTGCGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGCG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTACGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ATGCAGACAG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAACACCA TCTTCGCGTC GTTGGCTGTG AAATATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA CGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC CGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTTGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014957523.1
CDS	3319..9087
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRSGRRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVTASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILRSNAETATDLVVVDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCERLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPADADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISVANSSSAENENYCKHSTIVVPENAAHQGANRTSSLENHDQMSVNCRTLFSESPGKLQVEVATDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTEAGEAPPDRTASEQSPSPGHSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAPKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRTAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_015102037.1
Organism	Ovis aries(sheep)
Definition	Ovis aries dicer 1, ribonuclease type III (DICER1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015102037.1

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTGCTGCT CACTAAAGAG 
CTGTCTTACC AGATCAGGGG AGACTTCAAC AGAAGTGGCA GGAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAACT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC GTCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTT AGGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCGTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTA GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTACAG AAACACATCA AACACGAGCA AGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTAAGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CACCTGCCTC CCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAGTA CAAGCCGTAC GAGCGGCAGC AGTTTGAAAG CGTGGAATGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGTG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC ATTTACCAAT 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CGGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT TGATCTGCCC 
ACAGAGTATC GATCCTATGT TCAGTCTAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGATACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCAG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGACGA TGACGATGTG TTCCCACCAT ACGTGCTGAG GCCTGAGGAC 
GGTCCTCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG ATTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAGA 
CTGCACAAAA TTGGTGAACT GGATGACCAT TTGATGCCCG TTGGGAAGGA GACTGTTAAA 
TATGAGGAGG AGCTGGATCT GCACGATGAA GAAGAGACTA GTGTTCCAGG AAGACCGGGC 
TCCACGAAGC GAAGGCAGTG CTACCCGAAA GCAATCCCCG AATGTTTGAG GGAGAGCTAC 
CCCAAGCCCG GTCAGCCCTG TTACCTGTAC GTGATCGGCA TGGTCCTGAC GACGCCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CCGAGGACAC CACGAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATTACAAG ACTTCACCAG TATATATTCT CGCATATTCT TCGGCTTGAA 
AAACCTGCCC TAGAATTTAA ACCCGCAGAC GCGGACTCAG CCTACTGTGT TCTACCTCTT 
AACGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGCATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCCTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTTAA TCTTTTGACA 
CCTCGCCATT TGAATCAGAA GGGGAAAGCT CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AAAGCCTGCA GAATAAACAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTCTGTCTCC CCAGCATACT CTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACAG CCAGCGATGC TGGTGTGGGA 
GTCAGATCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAGTTGCT AACTCCTCTT CAGCAGAAAA TGAGAATTAC 
TGTAAGCATA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGCTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAGTTGA AGTTGCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCCGCC 
GTGCCTGGTA CTACAGAGGC TGGGGAGGCG CCCCCGGACA GGACGGCTTC TGAGCAGAGC 
CCTTCTCCTG GGCACTCCTC GAGGACGCTC GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
CTGACCCTTT CGAATGCCAG CGACGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTGA AGCACGCCAT CACCACGTAT CTCTTCTGCA CGTACCCCGA CGCGCACGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAGCTGG ACGATGACTT TGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCCCAAGG AGGACGCCGA CGATGAGGAC 
GACTTCCTGG AGTACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT CCTTTTTCCG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTGGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCGTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC TGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGCGT CGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA GAGCATAGCT 
GACTGCGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGCG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTACGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ATGCAGACAG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAACACCA TCTTCGCGTC GTTGGCTGTG AAATATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA CGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC CGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTTGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_012099009.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011954399.1 Ovis aries dicer 1, ribonuclease type III (DICER1), transcript variant X2, mRNA.

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CloneID	OSm14330
Clone ID Related Accession (Same CDS sequence)	XM_004017979.3 , XM_015102037.1 , XM_012099009.2 , XM_015102036.1
Accession Version	XM_012099009.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012099009.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTGCTGCT CACTAAAGAG 
CTGTCTTACC AGATCAGGGG AGACTTCAAC AGAAGTGGCA GGAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAACT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC GTCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTT AGGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCGTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTA GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTACAG AAACACATCA AACACGAGCA AGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTAAGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CACCTGCCTC CCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAGTA CAAGCCGTAC GAGCGGCAGC AGTTTGAAAG CGTGGAATGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGTG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC ATTTACCAAT 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CGGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT TGATCTGCCC 
ACAGAGTATC GATCCTATGT TCAGTCTAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGATACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCAG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGACGA TGACGATGTG TTCCCACCAT ACGTGCTGAG GCCTGAGGAC 
GGTCCTCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG ATTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAGA 
CTGCACAAAA TTGGTGAACT GGATGACCAT TTGATGCCCG TTGGGAAGGA GACTGTTAAA 
TATGAGGAGG AGCTGGATCT GCACGATGAA GAAGAGACTA GTGTTCCAGG AAGACCGGGC 
TCCACGAAGC GAAGGCAGTG CTACCCGAAA GCAATCCCCG AATGTTTGAG GGAGAGCTAC 
CCCAAGCCCG GTCAGCCCTG TTACCTGTAC GTGATCGGCA TGGTCCTGAC GACGCCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CCGAGGACAC CACGAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATTACAAG ACTTCACCAG TATATATTCT CGCATATTCT TCGGCTTGAA 
AAACCTGCCC TAGAATTTAA ACCCGCAGAC GCGGACTCAG CCTACTGTGT TCTACCTCTT 
AACGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGCATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCCTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTTAA TCTTTTGACA 
CCTCGCCATT TGAATCAGAA GGGGAAAGCT CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AAAGCCTGCA GAATAAACAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTCTGTCTCC CCAGCATACT CTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACAG CCAGCGATGC TGGTGTGGGA 
GTCAGATCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAGTTGCT AACTCCTCTT CAGCAGAAAA TGAGAATTAC 
TGTAAGCATA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGCTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAGTTGA AGTTGCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCCGCC 
GTGCCTGGTA CTACAGAGGC TGGGGAGGCG CCCCCGGACA GGACGGCTTC TGAGCAGAGC 
CCTTCTCCTG GGCACTCCTC GAGGACGCTC GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
CTGACCCTTT CGAATGCCAG CGACGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTGA AGCACGCCAT CACCACGTAT CTCTTCTGCA CGTACCCCGA CGCGCACGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAGCTGG ACGATGACTT TGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCCCAAGG AGGACGCCGA CGATGAGGAC 
GACTTCCTGG AGTACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT CCTTTTTCCG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTGGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCGTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC TGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGCGT CGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA GAGCATAGCT 
GACTGCGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGCG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTACGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ATGCAGACAG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAACACCA TCTTCGCGTC GTTGGCTGTG AAATATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA CGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC CGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTTGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011954399.1
CDS	2796..8564
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRSGRRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVTASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILRSNAETATDLVVVDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCERLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPADADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISVANSSSAENENYCKHSTIVVPENAAHQGANRTSSLENHDQMSVNCRTLFSESPGKLQVEVATDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTEAGEAPPDRTASEQSPSPGHSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAPKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRTAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_012099009.2
Organism	Ovis aries(sheep)
Definition	Ovis aries dicer 1, ribonuclease type III (DICER1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012099009.2

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTGCTGCT CACTAAAGAG 
CTGTCTTACC AGATCAGGGG AGACTTCAAC AGAAGTGGCA GGAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAACT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC GTCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTT AGGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCGTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTA GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTACAG AAACACATCA AACACGAGCA AGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTAAGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CACCTGCCTC CCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAGTA CAAGCCGTAC GAGCGGCAGC AGTTTGAAAG CGTGGAATGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGTG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC ATTTACCAAT 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CGGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT TGATCTGCCC 
ACAGAGTATC GATCCTATGT TCAGTCTAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGATACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCAG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGACGA TGACGATGTG TTCCCACCAT ACGTGCTGAG GCCTGAGGAC 
GGTCCTCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG ATTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAGA 
CTGCACAAAA TTGGTGAACT GGATGACCAT TTGATGCCCG TTGGGAAGGA GACTGTTAAA 
TATGAGGAGG AGCTGGATCT GCACGATGAA GAAGAGACTA GTGTTCCAGG AAGACCGGGC 
TCCACGAAGC GAAGGCAGTG CTACCCGAAA GCAATCCCCG AATGTTTGAG GGAGAGCTAC 
CCCAAGCCCG GTCAGCCCTG TTACCTGTAC GTGATCGGCA TGGTCCTGAC GACGCCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CCGAGGACAC CACGAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATTACAAG ACTTCACCAG TATATATTCT CGCATATTCT TCGGCTTGAA 
AAACCTGCCC TAGAATTTAA ACCCGCAGAC GCGGACTCAG CCTACTGTGT TCTACCTCTT 
AACGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGCATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCCTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTTAA TCTTTTGACA 
CCTCGCCATT TGAATCAGAA GGGGAAAGCT CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AAAGCCTGCA GAATAAACAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTCTGTCTCC CCAGCATACT CTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACAG CCAGCGATGC TGGTGTGGGA 
GTCAGATCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAGTTGCT AACTCCTCTT CAGCAGAAAA TGAGAATTAC 
TGTAAGCATA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGCTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAGTTGA AGTTGCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCCGCC 
GTGCCTGGTA CTACAGAGGC TGGGGAGGCG CCCCCGGACA GGACGGCTTC TGAGCAGAGC 
CCTTCTCCTG GGCACTCCTC GAGGACGCTC GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
CTGACCCTTT CGAATGCCAG CGACGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTGA AGCACGCCAT CACCACGTAT CTCTTCTGCA CGTACCCCGA CGCGCACGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAGCTGG ACGATGACTT TGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCCCAAGG AGGACGCCGA CGATGAGGAC 
GACTTCCTGG AGTACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT CCTTTTTCCG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTGGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCGTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC TGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGCGT CGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA GAGCATAGCT 
GACTGCGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGCG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTACGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ATGCAGACAG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAACACCA TCTTCGCGTC GTTGGCTGTG AAATATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA CGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC CGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTTGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_015102036.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014957522.1 Ovis aries dicer 1, ribonuclease type III (DICER1), transcript variant X3, mRNA.

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CloneID	OSm14330
Clone ID Related Accession (Same CDS sequence)	XM_004017979.3 , XM_015102037.1 , XM_012099009.2 , XM_015102036.1
Accession Version	XM_015102036.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTGCTGCT CACTAAAGAG 
CTGTCTTACC AGATCAGGGG AGACTTCAAC AGAAGTGGCA GGAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAACT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC GTCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTT AGGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCGTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTA GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTACAG AAACACATCA AACACGAGCA AGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTAAGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CACCTGCCTC CCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAGTA CAAGCCGTAC GAGCGGCAGC AGTTTGAAAG CGTGGAATGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGTG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC ATTTACCAAT 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CGGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT TGATCTGCCC 
ACAGAGTATC GATCCTATGT TCAGTCTAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGATACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCAG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGACGA TGACGATGTG TTCCCACCAT ACGTGCTGAG GCCTGAGGAC 
GGTCCTCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG ATTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAGA 
CTGCACAAAA TTGGTGAACT GGATGACCAT TTGATGCCCG TTGGGAAGGA GACTGTTAAA 
TATGAGGAGG AGCTGGATCT GCACGATGAA GAAGAGACTA GTGTTCCAGG AAGACCGGGC 
TCCACGAAGC GAAGGCAGTG CTACCCGAAA GCAATCCCCG AATGTTTGAG GGAGAGCTAC 
CCCAAGCCCG GTCAGCCCTG TTACCTGTAC GTGATCGGCA TGGTCCTGAC GACGCCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CCGAGGACAC CACGAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATTACAAG ACTTCACCAG TATATATTCT CGCATATTCT TCGGCTTGAA 
AAACCTGCCC TAGAATTTAA ACCCGCAGAC GCGGACTCAG CCTACTGTGT TCTACCTCTT 
AACGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGCATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCCTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTTAA TCTTTTGACA 
CCTCGCCATT TGAATCAGAA GGGGAAAGCT CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AAAGCCTGCA GAATAAACAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTCTGTCTCC CCAGCATACT CTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACAG CCAGCGATGC TGGTGTGGGA 
GTCAGATCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAGTTGCT AACTCCTCTT CAGCAGAAAA TGAGAATTAC 
TGTAAGCATA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGCTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAGTTGA AGTTGCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCCGCC 
GTGCCTGGTA CTACAGAGGC TGGGGAGGCG CCCCCGGACA GGACGGCTTC TGAGCAGAGC 
CCTTCTCCTG GGCACTCCTC GAGGACGCTC GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
CTGACCCTTT CGAATGCCAG CGACGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTGA AGCACGCCAT CACCACGTAT CTCTTCTGCA CGTACCCCGA CGCGCACGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAGCTGG ACGATGACTT TGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCCCAAGG AGGACGCCGA CGATGAGGAC 
GACTTCCTGG AGTACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT CCTTTTTCCG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTGGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCGTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC TGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGCGT CGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA GAGCATAGCT 
GACTGCGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGCG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTACGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ATGCAGACAG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAACACCA TCTTCGCGTC GTTGGCTGTG AAATATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA CGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC CGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTTGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014957522.1
CDS	3832..9600
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRSGRRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVTASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILRSNAETATDLVVVDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCERLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPADADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISVANSSSAENENYCKHSTIVVPENAAHQGANRTSSLENHDQMSVNCRTLFSESPGKLQVEVATDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTEAGEAPPDRTASEQSPSPGHSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAPKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRTAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_015102036.1
Organism	Ovis aries(sheep)
Definition	Ovis aries dicer 1, ribonuclease type III (DICER1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015102036.1

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTGCTGCT CACTAAAGAG 
CTGTCTTACC AGATCAGGGG AGACTTCAAC AGAAGTGGCA GGAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAACT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC GTCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTT AGGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCGTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTA GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTACAG AAACACATCA AACACGAGCA AGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTAAGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CACCTGCCTC CCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAGTA CAAGCCGTAC GAGCGGCAGC AGTTTGAAAG CGTGGAATGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGTG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC ATTTACCAAT 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CGGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT TGATCTGCCC 
ACAGAGTATC GATCCTATGT TCAGTCTAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGATACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCAG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGACGA TGACGATGTG TTCCCACCAT ACGTGCTGAG GCCTGAGGAC 
GGTCCTCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG ATTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAGA 
CTGCACAAAA TTGGTGAACT GGATGACCAT TTGATGCCCG TTGGGAAGGA GACTGTTAAA 
TATGAGGAGG AGCTGGATCT GCACGATGAA GAAGAGACTA GTGTTCCAGG AAGACCGGGC 
TCCACGAAGC GAAGGCAGTG CTACCCGAAA GCAATCCCCG AATGTTTGAG GGAGAGCTAC 
CCCAAGCCCG GTCAGCCCTG TTACCTGTAC GTGATCGGCA TGGTCCTGAC GACGCCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CCGAGGACAC CACGAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATTACAAG ACTTCACCAG TATATATTCT CGCATATTCT TCGGCTTGAA 
AAACCTGCCC TAGAATTTAA ACCCGCAGAC GCGGACTCAG CCTACTGTGT TCTACCTCTT 
AACGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGCATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCCTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTTAA TCTTTTGACA 
CCTCGCCATT TGAATCAGAA GGGGAAAGCT CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AAAGCCTGCA GAATAAACAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTCTGTCTCC CCAGCATACT CTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACAG CCAGCGATGC TGGTGTGGGA 
GTCAGATCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAGTTGCT AACTCCTCTT CAGCAGAAAA TGAGAATTAC 
TGTAAGCATA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGCTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAGTTGA AGTTGCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCCGCC 
GTGCCTGGTA CTACAGAGGC TGGGGAGGCG CCCCCGGACA GGACGGCTTC TGAGCAGAGC 
CCTTCTCCTG GGCACTCCTC GAGGACGCTC GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
CTGACCCTTT CGAATGCCAG CGACGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTGA AGCACGCCAT CACCACGTAT CTCTTCTGCA CGTACCCCGA CGCGCACGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAGCTGG ACGATGACTT TGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCCCAAGG AGGACGCCGA CGATGAGGAC 
GACTTCCTGG AGTACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT CCTTTTTCCG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTGGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCGTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC TGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGCGT CGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA GAGCATAGCT 
GACTGCGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGCG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTACGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ATGCAGACAG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAACACCA TCTTCGCGTC GTTGGCTGTG AAATATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA CGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC CGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTTGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_027957365.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027813166.1 Ovis aries dicer 1, ribonuclease III (DICER1), transcript variant X2, mRNA.

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CloneID	OSm68060
Clone ID Related Accession (Same CDS sequence)	XM_027957365.1 , XM_027957367.1 , XM_027957364.1
Accession Version	XM_027957365.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	endoribonuclease Dicer
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040269.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTGCTGCT CACTAAAGAG 
CTGTCTTACC AGATCAGGGG AGACTTCAAC AGAAGTGGCA GGAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAACT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTT AGGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCGTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTA GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTACAG AAACACATCA AACACGAGCA AGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTAAGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CACCTGCCTC CCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAGTA CAAGCCGTAC GAGCGGCAGC AGTTTGAAAG CGTGGAATGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGTG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC ATTTACCAAT 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CGGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT TGATCTGCCC 
ACAGAGTATC GATCCTATGT TCAGTCTAAG GGGAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGATACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCAG TTGATACCGG GGAGGCTGAC 
ACGGAGCCCG TGGTGGACGA TGACGATGTG TTCCCACCAT ACGTGCTGAG GCCTGAGGAC 
GGTCCTCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG ATTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAGA 
CTGCACAAAA TTGGTGAACT GGATGACCAT TTGATGCCCG TTGGGAAGGA GACTGTTAAA 
TATGAGGAGG AGCTGGATCT GCACGATGAA GAAGAGACTA GTGTTCCAGG AAGACCGGGC 
TCCACGAAGC GAAGGCAGTG CTACCCGAAA GCAATCCCCG AATGTTTGAG GGAGAGCTAC 
CCCAAGCCCG GTCAGCCCTG TTACCTGTAC GTGATCGGCA TGGTCCTGAC GACGCCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CCGAGGACAC CACGAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATTACAAG ACTTCACCAG TATATATTCT CGCATATTCT TCGGCTTGAA 
AAACCTGCCC TAGAATTTAA ACCCGCAGAC GCGGACTCAG CCTACTGTGT TCTACCTCTT 
AACGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGCATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCCTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTTAA TCTTTTGACA 
CCTCGCCATT TGAATCAGAA GGGGAAAGCT CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AAAGCCTGCA GAATAAACAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTCTGTCTCC CCAGCATACT CTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACAG CCAGCGATGC TGGTGTGGGA 
GTCAGATCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAGTTGCT AACTCCTCTT CAGCAGAAAA TGAGAATTAC 
TGTAAGCATA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGCTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAGTTGA AGTTGCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCCGCC 
GTGCCTGGTA CTACAGAGGC TGGGGAGGCG CCCCCGGACA GGACGGCTTC TGAGCAGAGC 
CCTTCTCCTG GGCACTCCTC GAGGACGCTC GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
CTGACCCTTT CGAATGCCAG CGACGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTGA AGCACGCCAT CACCACGTAT CTCTTCTGCA CGTACCCCGA CGCGCACGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAGCTGG ACGATGACTT TGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCCCAAGG AGGACGCCGA CGATGAGGAC 
GACTTCCTGG AGTACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT CCTTTTTCCG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTGGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCGTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC TGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGCGT CGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA GAGCATAGCT 
GACTGCGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGCG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTACGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ATGCAGACAG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAACACCA TCTTCGCGTC GTTGGCTGTG AAATATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA CGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC CGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTTGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027813166.1
CDS	510..6278
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRSGRRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVTASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILRSNAETATDLVVVDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCERLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPADADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISVANSSSAENENYCKHSTIVVPENAAHQGANRTSSLENHDQMSVNCRTLFSESPGKLQVEVATDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTEAGEAPPDRTASEQSPSPGHSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAPKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRTAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_027957365.1
Organism	Ovis aries(sheep)
Definition	Ovis aries dicer 1, ribonuclease III (DICER1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027957365.1

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTGCTGCT CACTAAAGAG 
CTGTCTTACC AGATCAGGGG AGACTTCAAC AGAAGTGGCA GGAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAACT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTT AGGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCGTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTA GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTACAG AAACACATCA AACACGAGCA AGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTAAGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CACCTGCCTC CCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAGTA CAAGCCGTAC GAGCGGCAGC AGTTTGAAAG CGTGGAATGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGTG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC ATTTACCAAT 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CGGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT TGATCTGCCC 
ACAGAGTATC GATCCTATGT TCAGTCTAAG GGGAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGATACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCAG TTGATACCGG GGAGGCTGAC 
ACGGAGCCCG TGGTGGACGA TGACGATGTG TTCCCACCAT ACGTGCTGAG GCCTGAGGAC 
GGTCCTCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG ATTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAGA 
CTGCACAAAA TTGGTGAACT GGATGACCAT TTGATGCCCG TTGGGAAGGA GACTGTTAAA 
TATGAGGAGG AGCTGGATCT GCACGATGAA GAAGAGACTA GTGTTCCAGG AAGACCGGGC 
TCCACGAAGC GAAGGCAGTG CTACCCGAAA GCAATCCCCG AATGTTTGAG GGAGAGCTAC 
CCCAAGCCCG GTCAGCCCTG TTACCTGTAC GTGATCGGCA TGGTCCTGAC GACGCCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CCGAGGACAC CACGAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATTACAAG ACTTCACCAG TATATATTCT CGCATATTCT TCGGCTTGAA 
AAACCTGCCC TAGAATTTAA ACCCGCAGAC GCGGACTCAG CCTACTGTGT TCTACCTCTT 
AACGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGCATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCCTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTTAA TCTTTTGACA 
CCTCGCCATT TGAATCAGAA GGGGAAAGCT CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AAAGCCTGCA GAATAAACAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTCTGTCTCC CCAGCATACT CTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACAG CCAGCGATGC TGGTGTGGGA 
GTCAGATCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAGTTGCT AACTCCTCTT CAGCAGAAAA TGAGAATTAC 
TGTAAGCATA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGCTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAGTTGA AGTTGCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCCGCC 
GTGCCTGGTA CTACAGAGGC TGGGGAGGCG CCCCCGGACA GGACGGCTTC TGAGCAGAGC 
CCTTCTCCTG GGCACTCCTC GAGGACGCTC GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
CTGACCCTTT CGAATGCCAG CGACGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTGA AGCACGCCAT CACCACGTAT CTCTTCTGCA CGTACCCCGA CGCGCACGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAGCTGG ACGATGACTT TGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCCCAAGG AGGACGCCGA CGATGAGGAC 
GACTTCCTGG AGTACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT CCTTTTTCCG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTGGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCGTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC TGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGCGT CGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA GAGCATAGCT 
GACTGCGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGCG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTACGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ATGCAGACAG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAACACCA TCTTCGCGTC GTTGGCTGTG AAATATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA CGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC CGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTTGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_027957367.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027813168.1 Ovis aries dicer 1, ribonuclease III (DICER1), transcript variant X3, mRNA.

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CloneID	OSm68060
Clone ID Related Accession (Same CDS sequence)	XM_027957365.1 , XM_027957367.1 , XM_027957364.1
Accession Version	XM_027957367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	endoribonuclease Dicer
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040269.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTGCTGCT CACTAAAGAG 
CTGTCTTACC AGATCAGGGG AGACTTCAAC AGAAGTGGCA GGAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAACT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTT AGGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCGTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTA GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTACAG AAACACATCA AACACGAGCA AGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTAAGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CACCTGCCTC CCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAGTA CAAGCCGTAC GAGCGGCAGC AGTTTGAAAG CGTGGAATGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGTG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC ATTTACCAAT 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CGGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT TGATCTGCCC 
ACAGAGTATC GATCCTATGT TCAGTCTAAG GGGAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGATACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCAG TTGATACCGG GGAGGCTGAC 
ACGGAGCCCG TGGTGGACGA TGACGATGTG TTCCCACCAT ACGTGCTGAG GCCTGAGGAC 
GGTCCTCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG ATTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAGA 
CTGCACAAAA TTGGTGAACT GGATGACCAT TTGATGCCCG TTGGGAAGGA GACTGTTAAA 
TATGAGGAGG AGCTGGATCT GCACGATGAA GAAGAGACTA GTGTTCCAGG AAGACCGGGC 
TCCACGAAGC GAAGGCAGTG CTACCCGAAA GCAATCCCCG AATGTTTGAG GGAGAGCTAC 
CCCAAGCCCG GTCAGCCCTG TTACCTGTAC GTGATCGGCA TGGTCCTGAC GACGCCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CCGAGGACAC CACGAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATTACAAG ACTTCACCAG TATATATTCT CGCATATTCT TCGGCTTGAA 
AAACCTGCCC TAGAATTTAA ACCCGCAGAC GCGGACTCAG CCTACTGTGT TCTACCTCTT 
AACGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGCATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCCTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTTAA TCTTTTGACA 
CCTCGCCATT TGAATCAGAA GGGGAAAGCT CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AAAGCCTGCA GAATAAACAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTCTGTCTCC CCAGCATACT CTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACAG CCAGCGATGC TGGTGTGGGA 
GTCAGATCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAGTTGCT AACTCCTCTT CAGCAGAAAA TGAGAATTAC 
TGTAAGCATA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGCTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAGTTGA AGTTGCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCCGCC 
GTGCCTGGTA CTACAGAGGC TGGGGAGGCG CCCCCGGACA GGACGGCTTC TGAGCAGAGC 
CCTTCTCCTG GGCACTCCTC GAGGACGCTC GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
CTGACCCTTT CGAATGCCAG CGACGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTGA AGCACGCCAT CACCACGTAT CTCTTCTGCA CGTACCCCGA CGCGCACGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAGCTGG ACGATGACTT TGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCCCAAGG AGGACGCCGA CGATGAGGAC 
GACTTCCTGG AGTACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT CCTTTTTCCG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTGGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCGTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC TGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGCGT CGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA GAGCATAGCT 
GACTGCGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGCG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTACGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ATGCAGACAG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAACACCA TCTTCGCGTC GTTGGCTGTG AAATATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA CGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC CGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTTGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027813168.1
CDS	317..6085
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRSGRRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVTASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILRSNAETATDLVVVDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCERLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPADADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISVANSSSAENENYCKHSTIVVPENAAHQGANRTSSLENHDQMSVNCRTLFSESPGKLQVEVATDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTEAGEAPPDRTASEQSPSPGHSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAPKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRTAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_027957367.1
Organism	Ovis aries(sheep)
Definition	Ovis aries dicer 1, ribonuclease III (DICER1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027957367.1

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTGCTGCT CACTAAAGAG 
CTGTCTTACC AGATCAGGGG AGACTTCAAC AGAAGTGGCA GGAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAACT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTT AGGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCGTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTA GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTACAG AAACACATCA AACACGAGCA AGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTAAGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CACCTGCCTC CCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAGTA CAAGCCGTAC GAGCGGCAGC AGTTTGAAAG CGTGGAATGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGTG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC ATTTACCAAT 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CGGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT TGATCTGCCC 
ACAGAGTATC GATCCTATGT TCAGTCTAAG GGGAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGATACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCAG TTGATACCGG GGAGGCTGAC 
ACGGAGCCCG TGGTGGACGA TGACGATGTG TTCCCACCAT ACGTGCTGAG GCCTGAGGAC 
GGTCCTCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG ATTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAGA 
CTGCACAAAA TTGGTGAACT GGATGACCAT TTGATGCCCG TTGGGAAGGA GACTGTTAAA 
TATGAGGAGG AGCTGGATCT GCACGATGAA GAAGAGACTA GTGTTCCAGG AAGACCGGGC 
TCCACGAAGC GAAGGCAGTG CTACCCGAAA GCAATCCCCG AATGTTTGAG GGAGAGCTAC 
CCCAAGCCCG GTCAGCCCTG TTACCTGTAC GTGATCGGCA TGGTCCTGAC GACGCCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CCGAGGACAC CACGAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATTACAAG ACTTCACCAG TATATATTCT CGCATATTCT TCGGCTTGAA 
AAACCTGCCC TAGAATTTAA ACCCGCAGAC GCGGACTCAG CCTACTGTGT TCTACCTCTT 
AACGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGCATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCCTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTTAA TCTTTTGACA 
CCTCGCCATT TGAATCAGAA GGGGAAAGCT CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AAAGCCTGCA GAATAAACAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTCTGTCTCC CCAGCATACT CTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACAG CCAGCGATGC TGGTGTGGGA 
GTCAGATCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAGTTGCT AACTCCTCTT CAGCAGAAAA TGAGAATTAC 
TGTAAGCATA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGCTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAGTTGA AGTTGCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCCGCC 
GTGCCTGGTA CTACAGAGGC TGGGGAGGCG CCCCCGGACA GGACGGCTTC TGAGCAGAGC 
CCTTCTCCTG GGCACTCCTC GAGGACGCTC GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
CTGACCCTTT CGAATGCCAG CGACGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTGA AGCACGCCAT CACCACGTAT CTCTTCTGCA CGTACCCCGA CGCGCACGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAGCTGG ACGATGACTT TGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCCCAAGG AGGACGCCGA CGATGAGGAC 
GACTTCCTGG AGTACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT CCTTTTTCCG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTGGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCGTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC TGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGCGT CGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA GAGCATAGCT 
GACTGCGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGCG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTACGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ATGCAGACAG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAACACCA TCTTCGCGTC GTTGGCTGTG AAATATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA CGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC CGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTTGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_027957364.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027813165.1 Ovis aries dicer 1, ribonuclease III (DICER1), transcript variant X1, mRNA.

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CloneID	OSm68060
Clone ID Related Accession (Same CDS sequence)	XM_027957365.1 , XM_027957367.1 , XM_027957364.1
Accession Version	XM_027957364.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	endoribonuclease Dicer
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040269.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTGCTGCT CACTAAAGAG 
CTGTCTTACC AGATCAGGGG AGACTTCAAC AGAAGTGGCA GGAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAACT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTT AGGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCGTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTA GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTACAG AAACACATCA AACACGAGCA AGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTAAGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CACCTGCCTC CCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAGTA CAAGCCGTAC GAGCGGCAGC AGTTTGAAAG CGTGGAATGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGTG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC ATTTACCAAT 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CGGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT TGATCTGCCC 
ACAGAGTATC GATCCTATGT TCAGTCTAAG GGGAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGATACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCAG TTGATACCGG GGAGGCTGAC 
ACGGAGCCCG TGGTGGACGA TGACGATGTG TTCCCACCAT ACGTGCTGAG GCCTGAGGAC 
GGTCCTCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG ATTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAGA 
CTGCACAAAA TTGGTGAACT GGATGACCAT TTGATGCCCG TTGGGAAGGA GACTGTTAAA 
TATGAGGAGG AGCTGGATCT GCACGATGAA GAAGAGACTA GTGTTCCAGG AAGACCGGGC 
TCCACGAAGC GAAGGCAGTG CTACCCGAAA GCAATCCCCG AATGTTTGAG GGAGAGCTAC 
CCCAAGCCCG GTCAGCCCTG TTACCTGTAC GTGATCGGCA TGGTCCTGAC GACGCCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CCGAGGACAC CACGAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATTACAAG ACTTCACCAG TATATATTCT CGCATATTCT TCGGCTTGAA 
AAACCTGCCC TAGAATTTAA ACCCGCAGAC GCGGACTCAG CCTACTGTGT TCTACCTCTT 
AACGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGCATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCCTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTTAA TCTTTTGACA 
CCTCGCCATT TGAATCAGAA GGGGAAAGCT CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AAAGCCTGCA GAATAAACAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTCTGTCTCC CCAGCATACT CTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACAG CCAGCGATGC TGGTGTGGGA 
GTCAGATCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAGTTGCT AACTCCTCTT CAGCAGAAAA TGAGAATTAC 
TGTAAGCATA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGCTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAGTTGA AGTTGCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCCGCC 
GTGCCTGGTA CTACAGAGGC TGGGGAGGCG CCCCCGGACA GGACGGCTTC TGAGCAGAGC 
CCTTCTCCTG GGCACTCCTC GAGGACGCTC GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
CTGACCCTTT CGAATGCCAG CGACGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTGA AGCACGCCAT CACCACGTAT CTCTTCTGCA CGTACCCCGA CGCGCACGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAGCTGG ACGATGACTT TGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCCCAAGG AGGACGCCGA CGATGAGGAC 
GACTTCCTGG AGTACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT CCTTTTTCCG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTGGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCGTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC TGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGCGT CGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA GAGCATAGCT 
GACTGCGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGCG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTACGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ATGCAGACAG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAACACCA TCTTCGCGTC GTTGGCTGTG AAATATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA CGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC CGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTTGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027813165.1
CDS	482..6250
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRSGRRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVTASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILRSNAETATDLVVVDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCERLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPADADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISVANSSSAENENYCKHSTIVVPENAAHQGANRTSSLENHDQMSVNCRTLFSESPGKLQVEVATDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTEAGEAPPDRTASEQSPSPGHSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAPKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRTAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_027957364.1
Organism	Ovis aries(sheep)
Definition	Ovis aries dicer 1, ribonuclease III (DICER1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027957364.1

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTGCTGCT CACTAAAGAG 
CTGTCTTACC AGATCAGGGG AGACTTCAAC AGAAGTGGCA GGAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAACT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTT AGGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCGTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTA GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTACAG AAACACATCA AACACGAGCA AGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTAAGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CACCTGCCTC CCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAGTA CAAGCCGTAC GAGCGGCAGC AGTTTGAAAG CGTGGAATGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGTG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC ATTTACCAAT 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CGGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TCCTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT TGATCTGCCC 
ACAGAGTATC GATCCTATGT TCAGTCTAAG GGGAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGATACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCAG TTGATACCGG GGAGGCTGAC 
ACGGAGCCCG TGGTGGACGA TGACGATGTG TTCCCACCAT ACGTGCTGAG GCCTGAGGAC 
GGTCCTCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG ATTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAGA 
CTGCACAAAA TTGGTGAACT GGATGACCAT TTGATGCCCG TTGGGAAGGA GACTGTTAAA 
TATGAGGAGG AGCTGGATCT GCACGATGAA GAAGAGACTA GTGTTCCAGG AAGACCGGGC 
TCCACGAAGC GAAGGCAGTG CTACCCGAAA GCAATCCCCG AATGTTTGAG GGAGAGCTAC 
CCCAAGCCCG GTCAGCCCTG TTACCTGTAC GTGATCGGCA TGGTCCTGAC GACGCCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CCGAGGACAC CACGAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATTACAAG ACTTCACCAG TATATATTCT CGCATATTCT TCGGCTTGAA 
AAACCTGCCC TAGAATTTAA ACCCGCAGAC GCGGACTCAG CCTACTGTGT TCTACCTCTT 
AACGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGCATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCCTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTTAA TCTTTTGACA 
CCTCGCCATT TGAATCAGAA GGGGAAAGCT CTTCCTCTAA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AAAGCCTGCA GAATAAACAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTCTGTCTCC CCAGCATACT CTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACAG CCAGCGATGC TGGTGTGGGA 
GTCAGATCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAGTTGCT AACTCCTCTT CAGCAGAAAA TGAGAATTAC 
TGTAAGCATA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGCTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAGTTGA AGTTGCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCCGCC 
GTGCCTGGTA CTACAGAGGC TGGGGAGGCG CCCCCGGACA GGACGGCTTC TGAGCAGAGC 
CCTTCTCCTG GGCACTCCTC GAGGACGCTC GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
CTGACCCTTT CGAATGCCAG CGACGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTGA AGCACGCCAT CACCACGTAT CTCTTCTGCA CGTACCCCGA CGCGCACGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAGCTGG ACGATGACTT TGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCCCAAGG AGGACGCCGA CGATGAGGAC 
GACTTCCTGG AGTACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCGCTTTCT CCTTTTTCCG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTGGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCGTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC TGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGCGT CGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA GAGCATAGCT 
GACTGCGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGCG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTACGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ATGCAGACAG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAACACCA TCTTCGCGTC GTTGGCTGTG AAATATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA CGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC CGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTTGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCCG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DICER1
Entrez Gene ID	101120020
Full Name	dicer 1, ribonuclease III
Gene Type	protein-coding
Organism	Ovis aries(sheep)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DICER1 cDNA ORF clone, Ovis aries(sheep)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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