MTHFD1 cDNA ORF clone, Equus caballus(horse)

Gene
Clones
gRNAs

Summary

Gene Symbol	MTHFD1
Entrez Gene ID	100062154
Full Name	methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1
General protein information	Preferred Names methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 Names C-1-tetrahydrofolate synthase, cytoplasmic
Gene Type	protein-coding
Organism	Equus caballus(horse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001499131.6	XP_001499181.2	C-1-tetrahydrofolate synthase, cytoplasmic
XM_001499131.5	XP_001499181.2	C-1-tetrahydrofolate synthase, cytoplasmic

Pathways from BioSystems
Gene Ontology

The following MTHFD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTHFD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHb13803	XM_001499131.6 Latest version!	Equus caballus methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
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OHb13803	XM_001499131.5	Equus caballus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (MTHFD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MTHFD1 ( XM_001499131.6 ) cDNA ORF clone, Equus caballus(horse) -> XP_001499181.2 Equus caballus methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), mRNA.

Price & Availability↑

CloneID	OHb13803
Clone ID Related Accession (Same CDS sequence)	XM_001499131.6 , XM_001499131.5
Accession Version	XM_001499131.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	C-1-tetrahydrofolate synthase, cytoplasmic
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009167.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_001499131.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCGG CGGAAATCCT GAACGGGAAG GTGGTCTCCG CGCAAATTAA GCAGAGACTG 
AAGAACCAGG TCACTCAGAT GAAGGAACAA GTACCGGGTT TCACACCGTG CCTGGCAATT 
TTACAGGTTG GCAACAGAGA TGATTCTAAT CTTTATATAA ACGTGAAGCT GAAGGCTGCT 
GAAGAGATTG GGATCAAAGC CACTCACATT AAGTTACCGA GAACGGCCAC AGAATCTGAG 
GTGTTGAAGT ACGTTACATC TTTGAATGAA GACTCCACTG TCCATGGGTT CATAGTGCAG 
CTGCCTTTAG ATTCAGAGAA TCCCATTAAC ACTGAAGCAG TGGTCAATGC TATTGCACCC 
GAAAAGGACG TGGATGGATT GACTAGCATC AATGCTGGAA AACTTGCTAG AGGGGACTTG 
AATGACTGTT TCATCCCTTG TACACCTAAA GGATGCTTGG AACTCATCAA AGAGACAGGG 
GTGCAGATTG CTGGAAGGCA TGCGGTGGTG GTTGGGCGCA GCAAAATAGT TGGTGCCCCG 
ATGCATGACT TGCTTCTGTG GAACCATGCC ACAGTGACCA CCTGCCACTC CAAGACTGCC 
AAGCTAGACG AGGAGGTAAA TAAAGGTGAC ATCCTCGTGG TTGCAGCTGG TCAGCCTGAA 
ATGGTGAAAG GGGAGTGGAT CAAACCTGGG GCAGTAGTCA TCGACTGTGG GATCAATTAC 
ATCCCAGATG ACACAAAACC AAATGGGAGA AAAGTTGTGG GTGATGTGGC ATACAGCGAG 
GCCAAAGAGA GGGCGGGCTT CATCACTCCT GTTCCTGGGG GTGTTGGGCC CATGACGGTG 
GCAATGCTGA TGCAGAGCAC AGTAGAGAGC GCAAAGCGTT TCCTGGAGAA ATTTAAGCCA 
GGAAAGTGGA TGATTGCGTA TAACAACCTT GACCTCAAGA CGCCTGTTCC GAGTGACATT 
CAAGTATCAC GATCTTGCAA GCCAAAGCCC ATTGGTAACC TGGCTCGAGA AATTGGTCTG 
CTGTCTGAAG AGGTGGAATT GTACGGTGAA ACAAAGGCCA AAATTCTGCT GTCTACACTG 
GAACGCCTGA AGCACCAGCC TGATGGAAAA TATGTGGTGG TGACTGGAAT AACTCCAACG 
CCCCTGGGAG AAGGGAAAAG CACAACCACA ATCGGGCTGG TGCAAGCCCT TGGCGCCCAC 
CTTCATCAGA ACGTCTTTGC GTGTGTGCGG CAGCCTTCTC AGGGCCCCAC CTTTGGAATA 
AAAGGTGGCG CTGCAGGAGG TGGCTACTCA CAGGTCATTC CAATGGAAGA GTTTAATCTC 
CACCTCACTG GTGACATCCA TGCCATCACT GCAGCTAATA ACCTTGTTGC CGCAGCCATC 
GATGCCCGGA TGTTCCATGA ACTGACCCAG ACAGACAAGG CTCTCTTTAA TCGTTTGGTA 
CCATCAGTAA ATGGAGTGAA AAAGTTCTCT GATATCCAAA TCCGAAGGTT ACAGAAACTA 
GGCATTGAAA AGACTGACCC CACCACACTG ACAGATGAAG AGATAAACAG ATTTGCAAGA 
TTGGATATTG ATCCAGAAAC GATAACTTGG CAAAGAGTGT TGGATACCAA TGATAGGTTC 
CTGAGGAAGA TCACAATTGG ACAGGCTTCA ACGGAGAAAG GGCACACACG GACGGCCCAG 
TTTGATATCT CTGTGGCCAG TGAAATCATG GCTGTCTTGG CTCTCACCAG TTCTCTAGAA 
GACATGAGAG ACAGACTGAG CAAAATGGTG GTGGCATCCA GTAAGAAAGG GGAACCCATC 
ACTACTGAAG ATCTGGGAGT GAGCGGCGCG CTGACGGTGC TCATGAAGGA TGCAATCAAG 
CCCAATCTCA TGCAGACGCT GGAGGGCACT CCAGTGTTTG TTCACGCGGG ACCGTTTGCC 
AACATCGCGC ATGGGAATTC CTCCATCATT GCAGACCGGA TTGCACTCAA GCTTGTTGGC 
CCCGAAGGGT TTGTAGTGAC TGAAGCAGGA TTTGGAGCAG ACATCGGAAT GGAAAAGTTT 
TTTAACATCA AGTGCCGGTA TTCTGGTCTC CGCCCTCACG TGGTGGTGCT TGTTGCCACT 
GTCAGGGCCC TTAAAATGCA CGGAGGCGGC CCCACGGTCA CTGCTGGGCT GCCTCTTCCC 
AAGGCTTACA CAGAGGAGAA CCTGGAGCTG GTTGAAAAAG GCTTCAGTAA CTTGAGGAAA 
CAAATTGAAA ATGCCAGAAT GTTTGGAGTT CCAGTAGTAG TGGCCGTGAA TGCATTCAGG 
ACAGATACGG AGGCCGAGCT GGACCTCATC AGCCACCTTG CCAAAGCGCA TGGGGCTTTC 
GACGCTGTGA AGTGCACTCA CTGGGCAGAA GGGGGCAGGG GGGCCCTGGC CCTGGCTCAG 
GCCGTCCAGA GAGCAGCTGA GGCACCCAGC AGCTTCCAGC TTCTTTATGA CCTCAAGCTC 
CCAGTTGAGG ATAAGATCAG GATCATTGCG CAGAAGATCT ACGGGGCAGA CGACATTGAA 
TTGCTGCCTG AAGCTCAGCA CAAAGCTGAA GTCTACACAA AGCAGGGCTT CGGGGATCTG 
CCCATCTGCA TGGCCAAAAC ACACTTGTCC TTGTCTCACA ATCCAGAGCA AAAAGGCGTG 
CCTACAGGCT TCGTCCTGCC CATTCGCGAC ATCCGTGCCA GCGTTGGGGC GGGTTTTCTG 
TACCCCCTGG TCGGAACGAT GAGCACAATG CCTGGACTCC CTACCCGGCC CTGTTTCTAT 
GATATTGATC TGGACCCTGA AACTGAGCAG GTCAATGGGC TGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001499181.2
CDS	117..2924
Translation	MAPAEILNGKVVSAQIKQRLKNQVTQMKEQVPGFTPCLAILQVGNRDDSNLYINVKLKAAEEIGIKATHIKLPRTATESEVLKYVTSLNEDSTVHGFIVQLPLDSENPINTEAVVNAIAPEKDVDGLTSINAGKLARGDLNDCFIPCTPKGCLELIKETGVQIAGRHAVVVGRSKIVGAPMHDLLLWNHATVTTCHSKTAKLDEEVNKGDILVVAAGQPEMVKGEWIKPGAVVIDCGINYIPDDTKPNGRKVVGDVAYSEAKERAGFITPVPGGVGPMTVAMLMQSTVESAKRFLEKFKPGKWMIAYNNLDLKTPVPSDIQVSRSCKPKPIGNLAREIGLLSEEVELYGETKAKILLSTLERLKHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLHQNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARMFHELTQTDKALFNRLVPSVNGVKKFSDIQIRRLQKLGIEKTDPTTLTDEEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQASTEKGHTRTAQFDISVASEIMAVLALTSSLEDMRDRLSKMVVASSKKGEPITTEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADRIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLRPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYTEENLELVEKGFSNLRKQIENARMFGVPVVVAVNAFRTDTEAELDLISHLAKAHGAFDAVKCTHWAEGGRGALALAQAVQRAAEAPSSFQLLYDLKLPVEDKIRIIAQKIYGADDIELLPEAQHKAEVYTKQGFGDLPICMAKTHLSLSHNPEQKGVPTGFVLPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDLDPETEQVNGLF

Target ORF information:

RefSeq Version	XM_001499131.6
Organism	Equus caballus(horse)
Definition	Equus caballus methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001499131.6

ORF Insert Sequence:

ATGGCGCCGG CGGAAATCCT GAACGGGAAG GTGGTCTCCG CGCAAATTAA GCAGAGACTG 
AAGAACCAGG TCACTCAGAT GAAGGAACAA GTACCGGGTT TCACACCGTG CCTGGCAATT 
TTACAGGTTG GCAACAGAGA TGATTCTAAT CTTTATATAA ACGTGAAGCT GAAGGCTGCT 
GAAGAGATTG GGATCAAAGC CACTCACATT AAGTTACCGA GAACGGCCAC AGAATCTGAG 
GTGTTGAAGT ACGTTACATC TTTGAATGAA GACTCCACTG TCCATGGGTT CATAGTGCAG 
CTGCCTTTAG ATTCAGAGAA TCCCATTAAC ACTGAAGCAG TGGTCAATGC TATTGCACCC 
GAAAAGGACG TGGATGGATT GACTAGCATC AATGCTGGAA AACTTGCTAG AGGGGACTTG 
AATGACTGTT TCATCCCTTG TACACCTAAA GGATGCTTGG AACTCATCAA AGAGACAGGG 
GTGCAGATTG CTGGAAGGCA TGCGGTGGTG GTTGGGCGCA GCAAAATAGT TGGTGCCCCG 
ATGCATGACT TGCTTCTGTG GAACCATGCC ACAGTGACCA CCTGCCACTC CAAGACTGCC 
AAGCTAGACG AGGAGGTAAA TAAAGGTGAC ATCCTCGTGG TTGCAGCTGG TCAGCCTGAA 
ATGGTGAAAG GGGAGTGGAT CAAACCTGGG GCAGTAGTCA TCGACTGTGG GATCAATTAC 
ATCCCAGATG ACACAAAACC AAATGGGAGA AAAGTTGTGG GTGATGTGGC ATACAGCGAG 
GCCAAAGAGA GGGCGGGCTT CATCACTCCT GTTCCTGGGG GTGTTGGGCC CATGACGGTG 
GCAATGCTGA TGCAGAGCAC AGTAGAGAGC GCAAAGCGTT TCCTGGAGAA ATTTAAGCCA 
GGAAAGTGGA TGATTGCGTA TAACAACCTT GACCTCAAGA CGCCTGTTCC GAGTGACATT 
CAAGTATCAC GATCTTGCAA GCCAAAGCCC ATTGGTAACC TGGCTCGAGA AATTGGTCTG 
CTGTCTGAAG AGGTGGAATT GTACGGTGAA ACAAAGGCCA AAATTCTGCT GTCTACACTG 
GAACGCCTGA AGCACCAGCC TGATGGAAAA TATGTGGTGG TGACTGGAAT AACTCCAACG 
CCCCTGGGAG AAGGGAAAAG CACAACCACA ATCGGGCTGG TGCAAGCCCT TGGCGCCCAC 
CTTCATCAGA ACGTCTTTGC GTGTGTGCGG CAGCCTTCTC AGGGCCCCAC CTTTGGAATA 
AAAGGTGGCG CTGCAGGAGG TGGCTACTCA CAGGTCATTC CAATGGAAGA GTTTAATCTC 
CACCTCACTG GTGACATCCA TGCCATCACT GCAGCTAATA ACCTTGTTGC CGCAGCCATC 
GATGCCCGGA TGTTCCATGA ACTGACCCAG ACAGACAAGG CTCTCTTTAA TCGTTTGGTA 
CCATCAGTAA ATGGAGTGAA AAAGTTCTCT GATATCCAAA TCCGAAGGTT ACAGAAACTA 
GGCATTGAAA AGACTGACCC CACCACACTG ACAGATGAAG AGATAAACAG ATTTGCAAGA 
TTGGATATTG ATCCAGAAAC GATAACTTGG CAAAGAGTGT TGGATACCAA TGATAGGTTC 
CTGAGGAAGA TCACAATTGG ACAGGCTTCA ACGGAGAAAG GGCACACACG GACGGCCCAG 
TTTGATATCT CTGTGGCCAG TGAAATCATG GCTGTCTTGG CTCTCACCAG TTCTCTAGAA 
GACATGAGAG ACAGACTGAG CAAAATGGTG GTGGCATCCA GTAAGAAAGG GGAACCCATC 
ACTACTGAAG ATCTGGGAGT GAGCGGCGCG CTGACGGTGC TCATGAAGGA TGCAATCAAG 
CCCAATCTCA TGCAGACGCT GGAGGGCACT CCAGTGTTTG TTCACGCGGG ACCGTTTGCC 
AACATCGCGC ATGGGAATTC CTCCATCATT GCAGACCGGA TTGCACTCAA GCTTGTTGGC 
CCCGAAGGGT TTGTAGTGAC TGAAGCAGGA TTTGGAGCAG ACATCGGAAT GGAAAAGTTT 
TTTAACATCA AGTGCCGGTA TTCTGGTCTC CGCCCTCACG TGGTGGTGCT TGTTGCCACT 
GTCAGGGCCC TTAAAATGCA CGGAGGCGGC CCCACGGTCA CTGCTGGGCT GCCTCTTCCC 
AAGGCTTACA CAGAGGAGAA CCTGGAGCTG GTTGAAAAAG GCTTCAGTAA CTTGAGGAAA 
CAAATTGAAA ATGCCAGAAT GTTTGGAGTT CCAGTAGTAG TGGCCGTGAA TGCATTCAGG 
ACAGATACGG AGGCCGAGCT GGACCTCATC AGCCACCTTG CCAAAGCGCA TGGGGCTTTC 
GACGCTGTGA AGTGCACTCA CTGGGCAGAA GGGGGCAGGG GGGCCCTGGC CCTGGCTCAG 
GCCGTCCAGA GAGCAGCTGA GGCACCCAGC AGCTTCCAGC TTCTTTATGA CCTCAAGCTC 
CCAGTTGAGG ATAAGATCAG GATCATTGCG CAGAAGATCT ACGGGGCAGA CGACATTGAA 
TTGCTGCCTG AAGCTCAGCA CAAAGCTGAA GTCTACACAA AGCAGGGCTT CGGGGATCTG 
CCCATCTGCA TGGCCAAAAC ACACTTGTCC TTGTCTCACA ATCCAGAGCA AAAAGGCGTG 
CCTACAGGCT TCGTCCTGCC CATTCGCGAC ATCCGTGCCA GCGTTGGGGC GGGTTTTCTG 
TACCCCCTGG TCGGAACGAT GAGCACAATG CCTGGACTCC CTACCCGGCC CTGTTTCTAT 
GATATTGATC TGGACCCTGA AACTGAGCAG GTCAATGGGC TGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFD1 ( XM_001499131.5 ) cDNA ORF clone, Equus caballus(horse) -> XP_001499181.2 Equus caballus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (MTHFD1), mRNA.

Price & Availability↑

CloneID	OHb13803
Clone ID Related Accession (Same CDS sequence)	XM_001499131.6 , XM_001499131.5
Accession Version	XM_001499131.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	C-1-tetrahydrofolate synthase, cytoplasmic
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867395.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 20, 2015 this sequence version replaced XM_001499131.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCGG CGGAAATCCT GAACGGGAAG GTGGTCTCCG CGCAAATTAA GCAGAGACTG 
AAGAACCAGG TCACTCAGAT GAAGGAACAA GTACCGGGTT TCACACCGTG CCTGGCAATT 
TTACAGGTTG GCAACAGAGA TGATTCTAAT CTTTATATAA ACGTGAAGCT GAAGGCTGCT 
GAAGAGATTG GGATCAAAGC CACTCACATT AAGTTACCGA GAACGGCCAC AGAATCTGAG 
GTGTTGAAGT ACGTTACATC TTTGAATGAA GACTCCACTG TCCATGGGTT CATAGTGCAG 
CTGCCTTTAG ATTCAGAGAA TCCCATTAAC ACTGAAGCAG TGGTCAATGC TATTGCACCC 
GAAAAGGACG TGGATGGATT GACTAGCATC AATGCTGGAA AACTTGCTAG AGGGGACTTG 
AATGACTGTT TCATCCCTTG TACACCTAAA GGATGCTTGG AACTCATCAA AGAGACAGGG 
GTGCAGATTG CTGGAAGGCA TGCGGTGGTG GTTGGGCGCA GCAAAATAGT TGGTGCCCCG 
ATGCATGACT TGCTTCTGTG GAACCATGCC ACAGTGACCA CCTGCCACTC CAAGACTGCC 
AAGCTAGACG AGGAGGTAAA TAAAGGTGAC ATCCTCGTGG TTGCAGCTGG TCAGCCTGAA 
ATGGTGAAAG GGGAGTGGAT CAAACCTGGG GCAGTAGTCA TCGACTGTGG GATCAATTAC 
ATCCCAGATG ACACAAAACC AAATGGGAGA AAAGTTGTGG GTGATGTGGC ATACAGCGAG 
GCCAAAGAGA GGGCGGGCTT CATCACTCCT GTTCCTGGGG GTGTTGGGCC CATGACGGTG 
GCAATGCTGA TGCAGAGCAC AGTAGAGAGC GCAAAGCGTT TCCTGGAGAA ATTTAAGCCA 
GGAAAGTGGA TGATTGCGTA TAACAACCTT GACCTCAAGA CGCCTGTTCC GAGTGACATT 
CAAGTATCAC GATCTTGCAA GCCAAAGCCC ATTGGTAACC TGGCTCGAGA AATTGGTCTG 
CTGTCTGAAG AGGTGGAATT GTACGGTGAA ACAAAGGCCA AAATTCTGCT GTCTACACTG 
GAACGCCTGA AGCACCAGCC TGATGGAAAA TATGTGGTGG TGACTGGAAT AACTCCAACG 
CCCCTGGGAG AAGGGAAAAG CACAACCACA ATCGGGCTGG TGCAAGCCCT TGGCGCCCAC 
CTTCATCAGA ACGTCTTTGC GTGTGTGCGG CAGCCTTCTC AGGGCCCCAC CTTTGGAATA 
AAAGGTGGCG CTGCAGGAGG TGGCTACTCA CAGGTCATTC CAATGGAAGA GTTTAATCTC 
CACCTCACTG GTGACATCCA TGCCATCACT GCAGCTAATA ACCTTGTTGC CGCAGCCATC 
GATGCCCGGA TGTTCCATGA ACTGACCCAG ACAGACAAGG CTCTCTTTAA TCGTTTGGTA 
CCATCAGTAA ATGGAGTGAA AAAGTTCTCT GATATCCAAA TCCGAAGGTT ACAGAAACTA 
GGCATTGAAA AGACTGACCC CACCACACTG ACAGATGAAG AGATAAACAG ATTTGCAAGA 
TTGGATATTG ATCCAGAAAC GATAACTTGG CAAAGAGTGT TGGATACCAA TGATAGGTTC 
CTGAGGAAGA TCACAATTGG ACAGGCTTCA ACGGAGAAAG GGCACACACG GACGGCCCAG 
TTTGATATCT CTGTGGCCAG TGAAATCATG GCTGTCTTGG CTCTCACCAG TTCTCTAGAA 
GACATGAGAG ACAGACTGAG CAAAATGGTG GTGGCATCCA GTAAGAAAGG GGAACCCATC 
ACTACTGAAG ATCTGGGAGT GAGCGGCGCG CTGACGGTGC TCATGAAGGA TGCAATCAAG 
CCCAATCTCA TGCAGACGCT GGAGGGCACT CCAGTGTTTG TTCACGCGGG ACCGTTTGCC 
AACATCGCGC ATGGGAATTC CTCCATCATT GCAGACCGGA TTGCACTCAA GCTTGTTGGC 
CCCGAAGGGT TTGTAGTGAC TGAAGCAGGA TTTGGAGCAG ACATCGGAAT GGAAAAGTTT 
TTTAACATCA AGTGCCGGTA TTCTGGTCTC CGCCCTCACG TGGTGGTGCT TGTTGCCACT 
GTCAGGGCCC TTAAAATGCA CGGAGGCGGC CCCACGGTCA CTGCTGGGCT GCCTCTTCCC 
AAGGCTTACA CAGAGGAGAA CCTGGAGCTG GTTGAAAAAG GCTTCAGTAA CTTGAGGAAA 
CAAATTGAAA ATGCCAGAAT GTTTGGAGTT CCAGTAGTAG TGGCCGTGAA TGCATTCAGG 
ACAGATACGG AGGCCGAGCT GGACCTCATC AGCCACCTTG CCAAAGCGCA TGGGGCTTTC 
GACGCTGTGA AGTGCACTCA CTGGGCAGAA GGGGGCAGGG GGGCCCTGGC CCTGGCTCAG 
GCCGTCCAGA GAGCAGCTGA GGCACCCAGC AGCTTCCAGC TTCTTTATGA CCTCAAGCTC 
CCAGTTGAGG ATAAGATCAG GATCATTGCG CAGAAGATCT ACGGGGCAGA CGACATTGAA 
TTGCTGCCTG AAGCTCAGCA CAAAGCTGAA GTCTACACAA AGCAGGGCTT CGGGGATCTG 
CCCATCTGCA TGGCCAAAAC ACACTTGTCC TTGTCTCACA ATCCAGAGCA AAAAGGCGTG 
CCTACAGGCT TCGTCCTGCC CATTCGCGAC ATCCGTGCCA GCGTTGGGGC GGGTTTTCTG 
TACCCCCTGG TCGGAACGAT GAGCACAATG CCTGGACTCC CTACCCGGCC CTGTTTCTAT 
GATATTGATC TGGACCCTGA AACTGAGCAG GTCAATGGGC TGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001499181.2
CDS	124..2931
Translation	MAPAEILNGKVVSAQIKQRLKNQVTQMKEQVPGFTPCLAILQVGNRDDSNLYINVKLKAAEEIGIKATHIKLPRTATESEVLKYVTSLNEDSTVHGFIVQLPLDSENPINTEAVVNAIAPEKDVDGLTSINAGKLARGDLNDCFIPCTPKGCLELIKETGVQIAGRHAVVVGRSKIVGAPMHDLLLWNHATVTTCHSKTAKLDEEVNKGDILVVAAGQPEMVKGEWIKPGAVVIDCGINYIPDDTKPNGRKVVGDVAYSEAKERAGFITPVPGGVGPMTVAMLMQSTVESAKRFLEKFKPGKWMIAYNNLDLKTPVPSDIQVSRSCKPKPIGNLAREIGLLSEEVELYGETKAKILLSTLERLKHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLHQNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARMFHELTQTDKALFNRLVPSVNGVKKFSDIQIRRLQKLGIEKTDPTTLTDEEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQASTEKGHTRTAQFDISVASEIMAVLALTSSLEDMRDRLSKMVVASSKKGEPITTEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADRIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLRPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYTEENLELVEKGFSNLRKQIENARMFGVPVVVAVNAFRTDTEAELDLISHLAKAHGAFDAVKCTHWAEGGRGALALAQAVQRAAEAPSSFQLLYDLKLPVEDKIRIIAQKIYGADDIELLPEAQHKAEVYTKQGFGDLPICMAKTHLSLSHNPEQKGVPTGFVLPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDLDPETEQVNGLF

Target ORF information:

RefSeq Version	XM_001499131.5
Organism	Equus caballus(horse)
Definition	Equus caballus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (MTHFD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001499131.5

ORF Insert Sequence:

ATGGCGCCGG CGGAAATCCT GAACGGGAAG GTGGTCTCCG CGCAAATTAA GCAGAGACTG 
AAGAACCAGG TCACTCAGAT GAAGGAACAA GTACCGGGTT TCACACCGTG CCTGGCAATT 
TTACAGGTTG GCAACAGAGA TGATTCTAAT CTTTATATAA ACGTGAAGCT GAAGGCTGCT 
GAAGAGATTG GGATCAAAGC CACTCACATT AAGTTACCGA GAACGGCCAC AGAATCTGAG 
GTGTTGAAGT ACGTTACATC TTTGAATGAA GACTCCACTG TCCATGGGTT CATAGTGCAG 
CTGCCTTTAG ATTCAGAGAA TCCCATTAAC ACTGAAGCAG TGGTCAATGC TATTGCACCC 
GAAAAGGACG TGGATGGATT GACTAGCATC AATGCTGGAA AACTTGCTAG AGGGGACTTG 
AATGACTGTT TCATCCCTTG TACACCTAAA GGATGCTTGG AACTCATCAA AGAGACAGGG 
GTGCAGATTG CTGGAAGGCA TGCGGTGGTG GTTGGGCGCA GCAAAATAGT TGGTGCCCCG 
ATGCATGACT TGCTTCTGTG GAACCATGCC ACAGTGACCA CCTGCCACTC CAAGACTGCC 
AAGCTAGACG AGGAGGTAAA TAAAGGTGAC ATCCTCGTGG TTGCAGCTGG TCAGCCTGAA 
ATGGTGAAAG GGGAGTGGAT CAAACCTGGG GCAGTAGTCA TCGACTGTGG GATCAATTAC 
ATCCCAGATG ACACAAAACC AAATGGGAGA AAAGTTGTGG GTGATGTGGC ATACAGCGAG 
GCCAAAGAGA GGGCGGGCTT CATCACTCCT GTTCCTGGGG GTGTTGGGCC CATGACGGTG 
GCAATGCTGA TGCAGAGCAC AGTAGAGAGC GCAAAGCGTT TCCTGGAGAA ATTTAAGCCA 
GGAAAGTGGA TGATTGCGTA TAACAACCTT GACCTCAAGA CGCCTGTTCC GAGTGACATT 
CAAGTATCAC GATCTTGCAA GCCAAAGCCC ATTGGTAACC TGGCTCGAGA AATTGGTCTG 
CTGTCTGAAG AGGTGGAATT GTACGGTGAA ACAAAGGCCA AAATTCTGCT GTCTACACTG 
GAACGCCTGA AGCACCAGCC TGATGGAAAA TATGTGGTGG TGACTGGAAT AACTCCAACG 
CCCCTGGGAG AAGGGAAAAG CACAACCACA ATCGGGCTGG TGCAAGCCCT TGGCGCCCAC 
CTTCATCAGA ACGTCTTTGC GTGTGTGCGG CAGCCTTCTC AGGGCCCCAC CTTTGGAATA 
AAAGGTGGCG CTGCAGGAGG TGGCTACTCA CAGGTCATTC CAATGGAAGA GTTTAATCTC 
CACCTCACTG GTGACATCCA TGCCATCACT GCAGCTAATA ACCTTGTTGC CGCAGCCATC 
GATGCCCGGA TGTTCCATGA ACTGACCCAG ACAGACAAGG CTCTCTTTAA TCGTTTGGTA 
CCATCAGTAA ATGGAGTGAA AAAGTTCTCT GATATCCAAA TCCGAAGGTT ACAGAAACTA 
GGCATTGAAA AGACTGACCC CACCACACTG ACAGATGAAG AGATAAACAG ATTTGCAAGA 
TTGGATATTG ATCCAGAAAC GATAACTTGG CAAAGAGTGT TGGATACCAA TGATAGGTTC 
CTGAGGAAGA TCACAATTGG ACAGGCTTCA ACGGAGAAAG GGCACACACG GACGGCCCAG 
TTTGATATCT CTGTGGCCAG TGAAATCATG GCTGTCTTGG CTCTCACCAG TTCTCTAGAA 
GACATGAGAG ACAGACTGAG CAAAATGGTG GTGGCATCCA GTAAGAAAGG GGAACCCATC 
ACTACTGAAG ATCTGGGAGT GAGCGGCGCG CTGACGGTGC TCATGAAGGA TGCAATCAAG 
CCCAATCTCA TGCAGACGCT GGAGGGCACT CCAGTGTTTG TTCACGCGGG ACCGTTTGCC 
AACATCGCGC ATGGGAATTC CTCCATCATT GCAGACCGGA TTGCACTCAA GCTTGTTGGC 
CCCGAAGGGT TTGTAGTGAC TGAAGCAGGA TTTGGAGCAG ACATCGGAAT GGAAAAGTTT 
TTTAACATCA AGTGCCGGTA TTCTGGTCTC CGCCCTCACG TGGTGGTGCT TGTTGCCACT 
GTCAGGGCCC TTAAAATGCA CGGAGGCGGC CCCACGGTCA CTGCTGGGCT GCCTCTTCCC 
AAGGCTTACA CAGAGGAGAA CCTGGAGCTG GTTGAAAAAG GCTTCAGTAA CTTGAGGAAA 
CAAATTGAAA ATGCCAGAAT GTTTGGAGTT CCAGTAGTAG TGGCCGTGAA TGCATTCAGG 
ACAGATACGG AGGCCGAGCT GGACCTCATC AGCCACCTTG CCAAAGCGCA TGGGGCTTTC 
GACGCTGTGA AGTGCACTCA CTGGGCAGAA GGGGGCAGGG GGGCCCTGGC CCTGGCTCAG 
GCCGTCCAGA GAGCAGCTGA GGCACCCAGC AGCTTCCAGC TTCTTTATGA CCTCAAGCTC 
CCAGTTGAGG ATAAGATCAG GATCATTGCG CAGAAGATCT ACGGGGCAGA CGACATTGAA 
TTGCTGCCTG AAGCTCAGCA CAAAGCTGAA GTCTACACAA AGCAGGGCTT CGGGGATCTG 
CCCATCTGCA TGGCCAAAAC ACACTTGTCC TTGTCTCACA ATCCAGAGCA AAAAGGCGTG 
CCTACAGGCT TCGTCCTGCC CATTCGCGAC ATCCGTGCCA GCGTTGGGGC GGGTTTTCTG 
TACCCCCTGG TCGGAACGAT GAGCACAATG CCTGGACTCC CTACCCGGCC CTGTTTCTAT 
GATATTGATC TGGACCCTGA AACTGAGCAG GTCAATGGGC TGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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MTHFD1 cDNA ORF clone, Equus caballus(horse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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