DYSF cDNA ORF clone, Equus caballus(horse)

The following DYSF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DYSF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHb06056 XM_001916966.4
Latest version!
Equus caballus dysferlin (DYSF), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OHb49775 XM_023618907.1
Latest version!
Equus caballus dysferlin (DYSF), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHb49776 XM_023618908.1
Latest version!
Equus caballus dysferlin (DYSF), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHb49777 XM_023618909.1
Latest version!
Equus caballus dysferlin (DYSF), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHb49778 XM_023618910.1
Latest version!
Equus caballus dysferlin (DYSF), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHb49779 XM_023618911.1
Latest version!
Equus caballus dysferlin (DYSF), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHb49780 XM_023618912.1
Latest version!
Equus caballus dysferlin (DYSF), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHb49781 XM_023618913.1
Latest version!
Equus caballus dysferlin (DYSF), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHb49782 XM_023618914.1
Latest version!
Equus caballus dysferlin (DYSF), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHb49783 XM_023618915.1
Latest version!
Equus caballus dysferlin (DYSF), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHb49784 XM_023618916.1
Latest version!
Equus caballus dysferlin (DYSF), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHb49785 XM_023618917.1
Latest version!
Equus caballus dysferlin (DYSF), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHb49786 XM_023618918.1
Latest version!
Equus caballus dysferlin (DYSF), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHb49787 XM_023618919.1
Latest version!
Equus caballus dysferlin (DYSF), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHb49788 XM_023618920.1
Latest version!
Equus caballus dysferlin (DYSF), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHb49789 XM_023618921.1
Latest version!
Equus caballus dysferlin (DYSF), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHb49790 XM_023618922.1
Latest version!
Equus caballus dysferlin (DYSF), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHb49791 XM_023618923.1
Latest version!
Equus caballus dysferlin (DYSF), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHb49792 XM_023618924.1
Latest version!
Equus caballus dysferlin (DYSF), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHb49793 XM_023618925.1
Latest version!
Equus caballus dysferlin (DYSF), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHb49794 XM_023618926.1
Latest version!
Equus caballus dysferlin (DYSF), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHb49795 XM_023618927.1
Latest version!
Equus caballus dysferlin (DYSF), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHb06056
    Clone ID Related Accession (Same CDS sequence) XM_001916966.4
    Accession Version XM_001916966.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6345bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product LOW QUALITY PROTEIN: dysferlin
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867379.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 20, 2015 this sequence version replaced XM_001916966.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 2 indels ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGTGCT GCCTGCTAGT GAAGGCCAGC AACCTTCCCA CTGTGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAA
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG GGTCCCCCTG
    GACCAGAGCT CTGAGCTTCA CGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
    TTCCTGGGAG AAGCCAAGGT CCCACTCCGG GAGGTCCTTG CCACCCCCAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACGCCTCT CCCTGGAGCT GTGCCCCTGT TCCCACCCTC TGTGCCGCTG
    GAACCCTCCC CGACTCTGCC CGATGTGGAT ATGCTGGCTG GTGGGGGACA GAGCCGGGCC
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAATGG
    CAGGCCCCCA CGGATACAGG AGGAGAGGAG GACACCGAGG ACCAGGGGCT CACGGGAGAT
    GAGGCAGAGC CATTCTTGGA TCAGAACGGA GCCCCAGGCC CCGGGGCTCC CACCACCCTG
    AAGAAGCCAC CTTCCCATCC TCCCCCCTAC CATCCTGGGG GGAAAAGGAA GAGAAGCACG
    CCTGCGCCCA GAAAGCTGCT TTCGGATAAA CCACAGGACT TCCAGATCAG GGTCCAGGTG
    ATCGAGGGGC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCGGCC
    AGGCAGACCA AGCGGACTCG GATTCACAGG GGAAACAGCC CGCTCTTCAA CGAGACTCTC
    TTCTTCAACG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGGCCGTCTT TATCACGGTG
    GTAGACTCCT GTTCGCTCCG GACAGATGCC CTCATCGGGG AGTTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCT
    GACGATTTCT CTGCTGGGCC CAAAGGCTAC CTGAAAGCAA GCCTTTGTGT GCTGGGGCCT
    GGAGACGAAG CTCCGCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAGC
    AATCTGCTCA GGCCAACTGG CATGGCCCTT CGAGGAGCGC ACTTCTGTCT GAAGGTCTTC
    AGGGCTGAGG ACTTACCACA GATGGACGAT GCCGTGGTGG ACAGCGTGAA GCAGATCTTC
    GGCTTTGACA GTAACAAGAA GAACCTGGTG GATCCCTTCA TCGAGGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ATGGCCAACC CTCAGTGGAA CCAGAGCATC
    ACGCTGCCTG TCATGTTTCC CTCCATGTCT GAAAAAATGA GGATTCGTGT CATAGACTGG
    GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAATATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGC AGAGGAGCCT GCAGGTGTGG TCAAGCCTCC TCCAGCCACG
    GACCTGGACG ACCACCTGGG CTTCCTCCCC ACCTTCGGGC CCTGCTATGT CAACCTCTAC
    GGCAGCCCCA GGGAGTTCAC TGGCTTCCCA GACCCCTATG CAGAGCTCAA CACGGGCAAG
    GGAGAAGGCG TGGCCTACCG AGGCCGGCTT CTGGTCTCCC TGGAGACCAA ACTGGTGGAG
    CAAGGCGAGC AGAAGGTGGA GGATCTCGCT GCAGATGACA TCCTTCGGGT GGAGAAGTAC
    CTCCGGAGGC GCAAGTACTC CCTCTTTGCT GCCTTCTACT CGGCCTGCAT GCTGCAGGAT
    GTCGACGATG CCATCCAGTT CGAGGTCAGC ATCGGAAACT ACGGGAACAA GTTTGACACG
    ACCTGCCTGC CGCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA CGGGTGCCAC
    TACTACTACT TACCCTGGGG CAACGTGAAG CCTGTGGTGG TGCTGTCATC CTACTGGGAA
    GACATCAGCC ACAGAGTCGA CACTCAGAAC CAGCTGCTCC GGATCGCCGA CCGGCTGGAA
    GCCGGCCTGG AGCAGGTCCA CCTGGCCCTG AAGGCGCGGT GCTCCACCGA GGATGTGGAC
    TCCCTGGTGG CTCAGCTGAT GGATGAGCTC ATTGCAGACT GCAGCCAGCC CCTGTGCAAC
    GTCCAAGAGA CGCCCTCTGC CACCCACCTG GACCAGTACC TGTACCGGCT GCGCACCCGT
    CACCTGAGCC AGATCAGTGA GGCGGCCCTG GCCTTGAAGC TCGGCCACAG TGAGCTCCCC
    GCCGCCCTTG AGCAGGCTGA GGACTGGCTC CTGCGTCTCC GTGCCCTGGC GGAGGAGCCC
    CAGAACAGCC TGCCTGATGT GGTCATCTGG ATGCTGCAGG GAGACAAGCG GGTGGCGTAC
    CAGCGGGTGC CCGCCCACGA GGTCCTCTTC TCCCGGCGGG GCGCCAACTA CTGTGGCAAG
    AATTGTGGGA AGCTGCAGAC GATCTTTCTG AAGTATCCAA TGGAAGGGGT GCCGGGAGCC
    CGGATGCCGG TGCAGATACG GGTGAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAG
    TTCAATCAGT TTGCTGAGGG CAAGCTCTCT GTCTTCGCTG AAACCTACGA GAACCAGACC
    AAGCTGGCCC TAGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAC
    GTCACAGGCA AGATCAAGCT ACCCAAGGAC AGCTTCCGCC CCTCGGCCGG CTGGGCCTGG
    GCTGGAGAGT GGTTCGTGTG TCCGGAGAAG ACCCTGCTCC ACGACACCGA TGCGGGCCAC
    CTGAGCTTTG TGGAGGAGGT GTTTGAGAAC CAGACCCGGC TGCCCGGAGG CCAGTGGATC
    TACATGAGTG ACAACTACAC AGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC
    GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCC
    GTTGACGAGC AAGGCTGGGA GTACAGCATC ACCATCCCTC CGGAACGGAA GCCAAGGCAC
    TGGGTCCCCG CCGAGAAGAT GTACTACACA CACCGTCGGC GGCGCTGGGT CCGCCTGCGC
    AGGAGGGACC TCAGCCAGAT GGAGGCGCTG AAGAGGCACA GGCAGGCGGA GGCGGAGGGC
    GAGGGCTGGG AGTACGCCTC GCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACC
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CACTGGAGAA GACAGGGCCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCGTGG TGGACGACAG GAGTGAAGAT
    TCGGTGTCCG TCTCCACCTT GAGCTTCGGC GTGAACAGAC CCACGATTTC CTGCATCTTC
    GACTACGGGA ACCGCTACCA TCTACGCTGC TACATGTACC AGGCCCGGGA CCTGCCCGCC
    ATGGACAAGG ACTCTTTTTC AGATCCCTAC GCAATCGTCT CCTTCCTGCA CCAGAGCCAG
    AAGACAGTGG TGGTGAAGAA CACCCTGAAC CCCACCTGGG ACCAGACACT CATCTTCTAT
    GAGATCGAGA TCTTTGGTGA GCCGCCCAGC ATCGCCGAGC AGCCGCCCAG CATCGTGNAG
    GAGCTATACG ACCATGACAC CTACGGTGCA GATGAGTTTA TGGGACGCTG CATCTGTCAG
    CCGACTCTGG AACGGATGCC CCGGCTGACC TGGTTCCCAC TGACTAGGGG CAGCCAGCCG
    GCGGGCGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCGGC CATCTACCAC
    ATTCCTGGTT TTGAGGTGCA GGATACATCA GGGATCCTGG AAGAGTCGGA GGACACAGAC
    CTGCCCTACC CGCCACCCCA GAGAGAGGCC AACATCTACA TGGTTCCCCA GAACATCAAG
    CCTGCTCTCC AGCGGACGGC CATCGAGATC CTGGCATGGG GCCTGCGGAA CATGAAGAGT
    TACCACCTGG CCAGTGTCTC CTCCCCCAGC CTCGTGGTGG AGTGCGGGGG CCAGACGGTG
    CAGTCCTGTG TCATCAGGAA CCTCCAGAAG AACCCCAACT TCGACGTCTG CACCCTCTTC
    ATGGAAGTGA TGCTGCCCAG GGAGGAGCTC TACTGTCCCC CCATCGTGGT CAAGGTCATC
    GATAACTGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCTG CTCCCTGGAG
    AGCTTCCTGT GTGACCCCTA CTCAGAGGAG AGTCCGTCCC CACAGGGCGG CCCAGATGAT
    GTGAGCTTAC TCAGTCCTGG GGAAGATGTG CTCATTGACA TTGATGACAA GGAGCCCCTC
    ATCCCTATGC AGGAGGAGGA GTTCATCGAT TGGTGGAGCA AATTCTTTGC CTCGATAGGG
    GAGAGGGAAA AGTGCGGGTC CTACTTGGAG AAGGACTTTG ACACTCTAAA GGTCTATGAC
    ACACAACTGG AGAATGTGGA GGCCTTTGAG GGCCTGTCTG ACTTTTGTAA CACCTTCAAG
    CTCTACCGGG GCAAGACTCA GGAGGAGATG GAAGACCCAT CTGTCGTTGG GGAATTTAAG
    GGCCTCTTCA AAATTTATCC CCTCCCAGAA GACCCGGCCA TCCCCATGCC CCCAAGACAG
    TTCCACCAGC TAGCTGCCCA GGGACCCCAG GAGTGCCTGG TCCGCATCTA CATCATCCGG
    GCATTCGGCC TGCAGCCCAA GGACCCCAAT GGGAAGTGTG ACCCTTACAT CAAGATCTCC
    ATAGGGAAGA AATCCGTGAG CGACCAGGAT AACTACATCC CCTGCACTCT GGAGCCTGTG
    TTTGGAAAGA TGTTCGAACT GACCTGCACT CTGCCTCTGG AGAAGGACCT GAAGATCACT
    CTCTATGACT ATGACCTCCT CTCCAAGGAC GAGATGATCG GGGAGACGGT CATCGACCTG
    GAGAATAGGC TGCTGTCCAA GTTTGGAGCT CGCTGCGGAC TCCCACAGAC CTACTGTGTC
    TCTGGACCGA ACCAGTGGAG GGACCAGCTC CGCCCCTCCC AGCTTCTCCA CCTGTTCTGC
    CAGCAGCAGA GAGTCAAGGC CCCCGTGTAC CAGACCGACC GTGTGGTGTT TGGGAATAAA
    GAATACACGA TTGAAGAAAT AGAGGCTGGG AGGGTCCTGA ATCCACACCT GGGCCCAGTG
    GAAGAGCGCC TGGCCTTGCA CGTCCTCCAG CAGCAGGGCT TGGTGCCCGA GCATGTGGAG
    TCCCGGCCCC TCTATAGCCC CCTGCAGCCG GACATTGAGC AGGGGAAGCT GCAGATGTGG
    ATCGACCTGT TTCCAAAGGC CCTGGGGCGG CCTGGACCTC CCTTCAACAT CACTCCACGG
    AGAGCCAGAA GGTTTTTCCT GCGTTGCATT ATCTGGAACA CCAAGGATGT GATCCTGGAC
    GACCTCAGCA TCACAGGAGA GAAGATGAGC GACATTTATG TGAAAGGTTG GATGGTTGGC
    TTTGAAGAAC ACAAGCAAAA GACAGATGTG CATTATCGGT CCCTGGGAGG CGAAGGGAAC
    TTTAACTGGA GGTTCATTTT CCCCTTTGAC TACCTGCTGG CTGAGCAAGT CTGCACTGTC
    TCCAAGAAGG ATGCTTTCTG GAAGCTGGAC AAGACCGAGA GCAAAATCCC AGCACGAGTG
    GTATTCCAGA TCTGGGACAA TGACAAGTTC TCCTTTGATG ATTTCCTGGG CTCCTTGCAG
    CTGGATCTCA ACCGCATGCC CAAGCCTGCC AAGACAGCCG AGAAGTGCTC CCTGGACCAG
    CTGGATGACA CTTTCCACCC AGAGTGGTTT GTGTCCCTTT TTGAACAGAA AACTGTGAAG
    GGCTGGTGGC CCTGTGTGGC GGAAGAGGGT GAGAAGAAGA AGCTGGCGGG CAAGCTGGAG
    ATGACCTTGG AGATTGTGGC GGAGAGTGAG CATGAGGAGC GGCCCGCTGG CCAGGGCCGG
    GATGAGCCCA ACATGAACCC CAAGCTCGAG GACCCAAGGC GCCCTGACAC CTCCTTCCTC
    TGGTTCACCT CCCCGTACAA GACTATGAAG TTCATCCTGT GGCGGCGTTT CCGGTGTGCC
    ATCATCCTCT TCATCATCCT CTTCATCCTA CTGCTGTTCT TGGGCATCTT CGTTTACGCC
    TTCCCGAACT ATGCTGCCAT GAAGCTGGTG AAGCCCTTCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001917001.2
    CDS179..6523
    Translation

    Target ORF information:

    RefSeq Version XM_001916966.4
    Organism Equus caballus(horse)
    Definition Equus caballus dysferlin (DYSF), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001916966.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGTGCT GCCTGCTAGT GAAGGCCAGC AACCTTCCCA CTGTGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAA
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG GGTCCCCCTG
    GACCAGAGCT CTGAGCTTCA CGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
    TTCCTGGGAG AAGCCAAGGT CCCACTCCGG GAGGTCCTTG CCACCCCCAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACGCCTCT CCCTGGAGCT GTGCCCCTGT TCCCACCCTC TGTGCCGCTG
    GAACCCTCCC CGACTCTGCC CGATGTGGAT ATGCTGGCTG GTGGGGGACA GAGCCGGGCC
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAATGG
    CAGGCCCCCA CGGATACAGG AGGAGAGGAG GACACCGAGG ACCAGGGGCT CACGGGAGAT
    GAGGCAGAGC CATTCTTGGA TCAGAACGGA GCCCCAGGCC CCGGGGCTCC CACCACCCTG
    AAGAAGCCAC CTTCCCATCC TCCCCCCTAC CATCCTGGGG GGAAAAGGAA GAGAAGCACG
    CCTGCGCCCA GAAAGCTGCT TTCGGATAAA CCACAGGACT TCCAGATCAG GGTCCAGGTG
    ATCGAGGGGC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCGGCC
    AGGCAGACCA AGCGGACTCG GATTCACAGG GGAAACAGCC CGCTCTTCAA CGAGACTCTC
    TTCTTCAACG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGGCCGTCTT TATCACGGTG
    GTAGACTCCT GTTCGCTCCG GACAGATGCC CTCATCGGGG AGTTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCT
    GACGATTTCT CTGCTGGGCC CAAAGGCTAC CTGAAAGCAA GCCTTTGTGT GCTGGGGCCT
    GGAGACGAAG CTCCGCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAGC
    AATCTGCTCA GGCCAACTGG CATGGCCCTT CGAGGAGCGC ACTTCTGTCT GAAGGTCTTC
    AGGGCTGAGG ACTTACCACA GATGGACGAT GCCGTGGTGG ACAGCGTGAA GCAGATCTTC
    GGCTTTGACA GTAACAAGAA GAACCTGGTG GATCCCTTCA TCGAGGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ATGGCCAACC CTCAGTGGAA CCAGAGCATC
    ACGCTGCCTG TCATGTTTCC CTCCATGTCT GAAAAAATGA GGATTCGTGT CATAGACTGG
    GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAATATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGC AGAGGAGCCT GCAGGTGTGG TCAAGCCTCC TCCAGCCACG
    GACCTGGACG ACCACCTGGG CTTCCTCCCC ACCTTCGGGC CCTGCTATGT CAACCTCTAC
    GGCAGCCCCA GGGAGTTCAC TGGCTTCCCA GACCCCTATG CAGAGCTCAA CACGGGCAAG
    GGAGAAGGCG TGGCCTACCG AGGCCGGCTT CTGGTCTCCC TGGAGACCAA ACTGGTGGAG
    CAAGGCGAGC AGAAGGTGGA GGATCTCGCT GCAGATGACA TCCTTCGGGT GGAGAAGTAC
    CTCCGGAGGC GCAAGTACTC CCTCTTTGCT GCCTTCTACT CGGCCTGCAT GCTGCAGGAT
    GTCGACGATG CCATCCAGTT CGAGGTCAGC ATCGGAAACT ACGGGAACAA GTTTGACACG
    ACCTGCCTGC CGCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA CGGGTGCCAC
    TACTACTACT TACCCTGGGG CAACGTGAAG CCTGTGGTGG TGCTGTCATC CTACTGGGAA
    GACATCAGCC ACAGAGTCGA CACTCAGAAC CAGCTGCTCC GGATCGCCGA CCGGCTGGAA
    GCCGGCCTGG AGCAGGTCCA CCTGGCCCTG AAGGCGCGGT GCTCCACCGA GGATGTGGAC
    TCCCTGGTGG CTCAGCTGAT GGATGAGCTC ATTGCAGACT GCAGCCAGCC CCTGTGCAAC
    GTCCAAGAGA CGCCCTCTGC CACCCACCTG GACCAGTACC TGTACCGGCT GCGCACCCGT
    CACCTGAGCC AGATCAGTGA GGCGGCCCTG GCCTTGAAGC TCGGCCACAG TGAGCTCCCC
    GCCGCCCTTG AGCAGGCTGA GGACTGGCTC CTGCGTCTCC GTGCCCTGGC GGAGGAGCCC
    CAGAACAGCC TGCCTGATGT GGTCATCTGG ATGCTGCAGG GAGACAAGCG GGTGGCGTAC
    CAGCGGGTGC CCGCCCACGA GGTCCTCTTC TCCCGGCGGG GCGCCAACTA CTGTGGCAAG
    AATTGTGGGA AGCTGCAGAC GATCTTTCTG AAGTATCCAA TGGAAGGGGT GCCGGGAGCC
    CGGATGCCGG TGCAGATACG GGTGAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAG
    TTCAATCAGT TTGCTGAGGG CAAGCTCTCT GTCTTCGCTG AAACCTACGA GAACCAGACC
    AAGCTGGCCC TAGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAC
    GTCACAGGCA AGATCAAGCT ACCCAAGGAC AGCTTCCGCC CCTCGGCCGG CTGGGCCTGG
    GCTGGAGAGT GGTTCGTGTG TCCGGAGAAG ACCCTGCTCC ACGACACCGA TGCGGGCCAC
    CTGAGCTTTG TGGAGGAGGT GTTTGAGAAC CAGACCCGGC TGCCCGGAGG CCAGTGGATC
    TACATGAGTG ACAACTACAC AGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC
    GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCC
    GTTGACGAGC AAGGCTGGGA GTACAGCATC ACCATCCCTC CGGAACGGAA GCCAAGGCAC
    TGGGTCCCCG CCGAGAAGAT GTACTACACA CACCGTCGGC GGCGCTGGGT CCGCCTGCGC
    AGGAGGGACC TCAGCCAGAT GGAGGCGCTG AAGAGGCACA GGCAGGCGGA GGCGGAGGGC
    GAGGGCTGGG AGTACGCCTC GCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACC
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CACTGGAGAA GACAGGGCCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCGTGG TGGACGACAG GAGTGAAGAT
    TCGGTGTCCG TCTCCACCTT GAGCTTCGGC GTGAACAGAC CCACGATTTC CTGCATCTTC
    GACTACGGGA ACCGCTACCA TCTACGCTGC TACATGTACC AGGCCCGGGA CCTGCCCGCC
    ATGGACAAGG ACTCTTTTTC AGATCCCTAC GCAATCGTCT CCTTCCTGCA CCAGAGCCAG
    AAGACAGTGG TGGTGAAGAA CACCCTGAAC CCCACCTGGG ACCAGACACT CATCTTCTAT
    GAGATCGAGA TCTTTGGTGA GCCGCCCAGC ATCGCCGAGC AGCCGCCCAG CATCGTGNAG
    GAGCTATACG ACCATGACAC CTACGGTGCA GATGAGTTTA TGGGACGCTG CATCTGTCAG
    CCGACTCTGG AACGGATGCC CCGGCTGACC TGGTTCCCAC TGACTAGGGG CAGCCAGCCG
    GCGGGCGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCGGC CATCTACCAC
    ATTCCTGGTT TTGAGGTGCA GGATACATCA GGGATCCTGG AAGAGTCGGA GGACACAGAC
    CTGCCCTACC CGCCACCCCA GAGAGAGGCC AACATCTACA TGGTTCCCCA GAACATCAAG
    CCTGCTCTCC AGCGGACGGC CATCGAGATC CTGGCATGGG GCCTGCGGAA CATGAAGAGT
    TACCACCTGG CCAGTGTCTC CTCCCCCAGC CTCGTGGTGG AGTGCGGGGG CCAGACGGTG
    CAGTCCTGTG TCATCAGGAA CCTCCAGAAG AACCCCAACT TCGACGTCTG CACCCTCTTC
    ATGGAAGTGA TGCTGCCCAG GGAGGAGCTC TACTGTCCCC CCATCGTGGT CAAGGTCATC
    GATAACTGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCTG CTCCCTGGAG
    AGCTTCCTGT GTGACCCCTA CTCAGAGGAG AGTCCGTCCC CACAGGGCGG CCCAGATGAT
    GTGAGCTTAC TCAGTCCTGG GGAAGATGTG CTCATTGACA TTGATGACAA GGAGCCCCTC
    ATCCCTATGC AGGAGGAGGA GTTCATCGAT TGGTGGAGCA AATTCTTTGC CTCGATAGGG
    GAGAGGGAAA AGTGCGGGTC CTACTTGGAG AAGGACTTTG ACACTCTAAA GGTCTATGAC
    ACACAACTGG AGAATGTGGA GGCCTTTGAG GGCCTGTCTG ACTTTTGTAA CACCTTCAAG
    CTCTACCGGG GCAAGACTCA GGAGGAGATG GAAGACCCAT CTGTCGTTGG GGAATTTAAG
    GGCCTCTTCA AAATTTATCC CCTCCCAGAA GACCCGGCCA TCCCCATGCC CCCAAGACAG
    TTCCACCAGC TAGCTGCCCA GGGACCCCAG GAGTGCCTGG TCCGCATCTA CATCATCCGG
    GCATTCGGCC TGCAGCCCAA GGACCCCAAT GGGAAGTGTG ACCCTTACAT CAAGATCTCC
    ATAGGGAAGA AATCCGTGAG CGACCAGGAT AACTACATCC CCTGCACTCT GGAGCCTGTG
    TTTGGAAAGA TGTTCGAACT GACCTGCACT CTGCCTCTGG AGAAGGACCT GAAGATCACT
    CTCTATGACT ATGACCTCCT CTCCAAGGAC GAGATGATCG GGGAGACGGT CATCGACCTG
    GAGAATAGGC TGCTGTCCAA GTTTGGAGCT CGCTGCGGAC TCCCACAGAC CTACTGTGTC
    TCTGGACCGA ACCAGTGGAG GGACCAGCTC CGCCCCTCCC AGCTTCTCCA CCTGTTCTGC
    CAGCAGCAGA GAGTCAAGGC CCCCGTGTAC CAGACCGACC GTGTGGTGTT TGGGAATAAA
    GAATACACGA TTGAAGAAAT AGAGGCTGGG AGGGTCCTGA ATCCACACCT GGGCCCAGTG
    GAAGAGCGCC TGGCCTTGCA CGTCCTCCAG CAGCAGGGCT TGGTGCCCGA GCATGTGGAG
    TCCCGGCCCC TCTATAGCCC CCTGCAGCCG GACATTGAGC AGGGGAAGCT GCAGATGTGG
    ATCGACCTGT TTCCAAAGGC CCTGGGGCGG CCTGGACCTC CCTTCAACAT CACTCCACGG
    AGAGCCAGAA GGTTTTTCCT GCGTTGCATT ATCTGGAACA CCAAGGATGT GATCCTGGAC
    GACCTCAGCA TCACAGGAGA GAAGATGAGC GACATTTATG TGAAAGGTTG GATGGTTGGC
    TTTGAAGAAC ACAAGCAAAA GACAGATGTG CATTATCGGT CCCTGGGAGG CGAAGGGAAC
    TTTAACTGGA GGTTCATTTT CCCCTTTGAC TACCTGCTGG CTGAGCAAGT CTGCACTGTC
    TCCAAGAAGG ATGCTTTCTG GAAGCTGGAC AAGACCGAGA GCAAAATCCC AGCACGAGTG
    GTATTCCAGA TCTGGGACAA TGACAAGTTC TCCTTTGATG ATTTCCTGGG CTCCTTGCAG
    CTGGATCTCA ACCGCATGCC CAAGCCTGCC AAGACAGCCG AGAAGTGCTC CCTGGACCAG
    CTGGATGACA CTTTCCACCC AGAGTGGTTT GTGTCCCTTT TTGAACAGAA AACTGTGAAG
    GGCTGGTGGC CCTGTGTGGC GGAAGAGGGT GAGAAGAAGA AGCTGGCGGG CAAGCTGGAG
    ATGACCTTGG AGATTGTGGC GGAGAGTGAG CATGAGGAGC GGCCCGCTGG CCAGGGCCGG
    GATGAGCCCA ACATGAACCC CAAGCTCGAG GACCCAAGGC GCCCTGACAC CTCCTTCCTC
    TGGTTCACCT CCCCGTACAA GACTATGAAG TTCATCCTGT GGCGGCGTTT CCGGTGTGCC
    ATCATCCTCT TCATCATCCT CTTCATCCTA CTGCTGTTCT TGGGCATCTT CGTTTACGCC
    TTCCCGAACT ATGCTGCCAT GAAGCTGGTG AAGCCCTTCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb49775
    Clone ID Related Accession (Same CDS sequence) XM_023618907.1
    Accession Version XM_023618907.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6450bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product dysferlin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009158.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGTGCT GCCTGCTAGT GAAGGCCAGC AACCTTCCCA CTGTGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAA
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG GGTCCCCCTG
    GACCAGAGCT CTGAGCTTCA CGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
    TTCCTGGGAG AAGCCAAGGT CCCACTCCGG GAGGTCCTTG CCACCCCCAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACGCCTCT CCCTGGAGCT GTGCCCCTGT TCCCACCCTC TGTGCCGCTG
    GAACCCTCCC CGACTCTGCC CGATGTGGAT ATGCTGGCTG GTGGGGGACA GAGCCGGGCC
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAATGG
    CAGGCCCCCA CGGATACAGG AGGAGAGGAG GACACCGAGG ACCAGGGGCT CACGGGAGAT
    GAGGCAGAGC CATTCTTGGA TCAGAACGGA GCCCCAGGCC CCGGGGCTCC CACCACCCTG
    AAGAAGCCAC CTTCCCATCC TCCCCCCTAC CATCCTGGGG GGAAAAGGAA GAGAAGCACG
    CCTGCGCCCA GAAAGCTGCT TTCGGATAAA CCACAGGACT TCCAGATCAG GGTCCAGGTG
    ATCGAGGGGC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCGGCC
    AGGCAGACCA AGCGGACTCG GATTCACAGG GGAAACAGCC CGCTCTTCAA CGAGACTCTC
    TTCTTCAACG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGGCCGTCTT TATCACGGTG
    GTAGACTCCT GTTCGCTCCG GACAGATGCC CTCATCGGGG AGTTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCT
    GACGATTTCT CTGCTGGGCC CAAAGGCTAC CTGAAAGCAA GCCTTTGTGT GCTGGGGCCT
    GGAGACGAAG CTCCGCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAGC
    AATCTGCTCA GGCCAACTGG CATGGCCCTT CGAGGAGCGC ACTTCTGTCT GAAGGTCTTC
    AGGGCTGAGG ACTTACCACA GATGGACGAT GCCGTGGTGG ACAGCGTGAA GCAGATCTTC
    GGCTTTGACA GTAACAAGAA GAACCTGGTG GATCCCTTCG TCGAGGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ATGGCCAACC CTCAGTGGAA CCAGAGCATC
    ACGCTGCCTG TCATGTTTCC CTCCATGTCT GAAAAAATGA GGATTCGTGT CATAGACTGG
    GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAATATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGC AGAGGAGCCT GCAGGTGTGG TCAAGCCTCC TCCAGCCACG
    GACCTGGACG ACCACCTGGG CTTCCTCCCC ACCTTCGGGC CCTGCTATGT CAACCTCTAC
    GGCAGCCCCA GGGAGTTCAC TGGCTTCCCA GACCCCTATG CAGAGCTCAA CACGGGCAAG
    GGAGAAGGCG TGGCCTACCG AGGCCGGCTT CTGGTCTCCC TGGAGACCAA ACTGGTGGAG
    CAAGGCGAGC AGAAGGTGGA GGATCTCGCT GCAGATGACA TCCTTCGGGT GGAGAAGTAC
    CTCCGGAGGC GCAAGTACTC CCTCTTTGCT GCCTTCTACT CGGCCTGCAT GCTGCAGGAT
    GTCGACGATG CCATCCAGTT CGAGGTCAGC ATCGGAAACT ACGGGAACAA GTTTGACACG
    ACCTGCCTGC CGCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA CGGGTGCCAC
    TACTACTACT TACCCTGGGG CAACGTGAAG CCTGTGGTGG TGCTGTCATC CTACTGGGAA
    GACATCAGCC ACAGAGTCGA CACTCAGAAC CAGCTGCTCC GGATCGCCGA CCGGCTGGAA
    GCCGGCCTGG AGCAGGTCCA CCTGGCCCTG AAGGCGCGGT GCTCCACCGA GGATGTGGAC
    TCCCTGGTGG CTCAGCTGAT GGATGAGCTC ATTGCAGACT GCAGCCAGCC CCTGTGCAAC
    GTCCAAGAGA CGCCCTCTGC CACCCACCTG GACCAGTACC TGTACCGGCT GCGCACCCGT
    CACCTGAGCC AGATCAGTGA GGCGGCCCTG GCCTTGAAGC TCGGCCACAG TGAGCTCCCC
    GCCGCCCTTG AGCAGGCTGA GGACTGGCTC CTGCGTCTCC GTGCCCTGGC GGAGGAGCCC
    CAGAACAGCC TGCCTGACGT GGTCATCTGG ATGCTGCAGG GAGACAAGCG GGTGGCGTAC
    CAGCGGGTGC CCGCCCACGA GGTCCTCTTC TCCCGGCGGG GCGCCAACTA CTGTGGCAAG
    AATTGTGGGA AGCTGCAGAC GATCTTTCTG AAGTATCCAA TGGAAGGGGT GCCGGGAGCC
    CGGATGCCGG TGCAGATACG GGTGAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAG
    TTCAATCAGT TTGCTGAGGG CAAGCTCTCT GTCTTCGCTG AAACCTACGA GAACCAGACC
    AAGCTGGCCC TAGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAC
    GTCACAGGCA AGATCAAGCT ACCCAAGGAC AGCTTCCGCC CCTCGGCCGG CTGGGCCTGG
    GCTGGAGAGT GGTTCGTGTG TCCGGAGAAG ACCCTACTCC ACGACACCGA TGCGGGCCAC
    CTGAGCTTTG TGGAGGAGGT GTTTGAGAAC CAGACCCGGC TGCCCGGAGG CCAGTGGATC
    TACATGAGTG ACAACTACAC AGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC
    GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCC
    GTTGACGAGC AAGGCTGGGA GTACAGCATC ACCATCCCTC CGGAACGGAA GCCAAGGCAC
    TGGGTCCCTG CCGAGAAGAT GTACTACACA CACCGTCGGC GGCGCTGGGT CCGCCTGCGC
    AGGAGGGACC TCAGCCAGAT GGAGGCGCTG AAGAGGCACA GGCAGGCGGA GGCGGAGGGC
    GAGGGCTGGG AGTACGCCTC GCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACC
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CACTGGAGAA GACAGGGCCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCGTGG TGGACGACAG GAGTGAAGAT
    TCGGTGTCCG TCTCCACCTT GAGCTTCGGC GTGAACAGAC CCACGATTTC CTGCATCTTC
    GACTACGGGA ACCGCTACCA TCTACGCTGC TACATGTACC AGGCCCGGGA CCTGCCCGCC
    ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCAATCGTCT CCTTCCTGCA CCAGAGCCAG
    AAGACAGTGG TGGTGAAGAA CACCCTGAAC CCCACCTGGG ACCAGACACT CATCTTCTAT
    GAGATCGAGA TCTTTGGTGA GCCGCCCAGC ATCGCCGAGC AGCCGCCCAG CATCGTGGTG
    GAGCTATACG ACCATGACAC CTACGGTGCA GATGAGTTTA TGGGACGCTG CATCTGTCAG
    CCGACTCTGG AACGGATGCC CCGGCTGACC TGGTTCCCAC TGACTAGGGG CAGCCAGCCG
    GCGGGCGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCGGC CATCTACCAC
    ATTCCTGGTT TTGAGGTGCA GGATACATCA GGGATCCTGG AAGAGCTGTG TGCACCTGCC
    TTCTTCCTCC AGGGCCTCCT CCAAACGTCG GAGGACACAG ACCTGCCCTA CCCGCCACCC
    CAGAGAGAGG CCAACATCTA CATGGTTCCC CAGAACATCA AGCCTGCTCT CCAGCGGACG
    GCCATCGAGA TCCTGGCATG GGGCCTGCGG AACATGAAGA GTTACCACCT GGCCAGTGTC
    TCCTCCCCCA GCCTCGTGGT GGAGTGCGGG GGCCAGACGG TGCAGTCCTG TGTCATCAGG
    AACCTCCAGA AGAACCCCAA CTTCGACGTC TGCACCCTCT TCATGGAAGT GATGCTGCCC
    AGGGAGGAGC TCTACTGTCC CCCCATCGTG GTCAAGGTCA TCGATAACTG CCAGTTTGGC
    CGCCGGCCTG TGGTGGGCCA GTGTACCATC TGCTCCCTGG AGAGCTTCCT GTGTGACCCC
    TACTCAGAGG AGAGTCCGTC CCCACAGGGC GGCCCAGATG ATGTGAGCTT ACTCAGTCCT
    GGGGAAGATG TGCTCATTGA CATTGATGAC AAGGAGCCCC TCATCCCTAT GCAGCTTGCA
    GATGGTCTGT CGGGCTCGGC CCCCACTAAC ATGGCATCTT CTCCGTCCAG TCCTCATGAG
    GAGGAGTTCA TCGATTGGTG GAGCAAATTC TTTGCCTCGA TAGGGGAGAG GGAAAAGTGC
    GGGTCCTACT TGGAGAAGGA CTTTGACACT CTAAAGGTCT ATGACACACA ACTGGAGAAT
    GTGGAGGCCT TTGAGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG
    ACTCAGGAGG AGATGGAAGA CCCATCTGTC GTTGGGGAAT TTAAGGGCCT CTTCAAAATT
    TATCCCCTCC CAGAAGACCC GGCCATCCCC ATGCCCCCAA GACAGTTCCA CCAGCTAGCT
    GCCCAGGGAC CCCAGGAGTG CCTGGTCCGC ATCTACATCA TCCGGGCATT CGGCCTGCAG
    CCCAAGGACC CCAATGGGAA GTGTGACCCT TACATCAAGA TCTCCATAGG GAAGAAATCC
    GTGAGCGACC AGGATAACTA CATCCCCTGC ACTCTGGAGC CTGTGTTTGG AAAGATGTTC
    GAACTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACTCTCTA TGACTATGAC
    CTCCTCTCCA AGGACGAGAT GATCGGGGAG ACGGTCATCG ACCTGGAGAA TAGGCTGCTG
    TCCAAGTTTG GAGCTCGCTG CGGACTCCCA CAGACCTACT GTGTCTCTGG ACCGAACCAG
    TGGAGGGACC AGCTCCGCCC CTCCCAGCTT CTCCACCTGT TCTGCCAGCA GCAGAGAGTC
    AAGGCCCCCG TGTACCAGAC CGACCGTGTG GTGTTTGGGA ATAAAGAATA CACGATTGAA
    GAAATAGAGG CTGGGAGGGT CCTGAATCCA CACCTGGGCC CAGTGGAAGA GCGCCTGGCC
    TTGCACGTCC TCCAGCAGCA GGGCTTGGTG CCCGAGCATG TGGAGTCCCG GCCCCTCTAT
    AGCCCCCTGC AGCCGGACAT TGAGCAGGGG AAGCTGCAGA TGTGGATCGA CCTGTTTCCA
    AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC AACATCACTC CACGGAGAGC CAGAAGGTTT
    TTCCTGCGTT GCATTATCTG GAACACCAAG GATGTGATCC TGGACGACCT CAGCATCACA
    GGAGAGAAGA TGAGCGACAT TTATGTGAAA GGTTGGATGG TTGGCTTTGA AGAACACAAG
    CAAAAGACAG ATGTGCATTA TCGGTCCCTG GGAGGCGAAG GGAACTTTAA CTGGAGGTTC
    ATTTTCCCCT TTGACTACCT GCTGGCTGAG CAAGTCTGCA CCGTCTCCAA GAAGGATGCT
    TTCTGGAAGC TGGACAAGAC CGAGAGCAAA ATCCCAGCAC GAGTGGTATT CCAGATCTGG
    GACAATGACA AGTTCTCCTT TGATGATTTC CTGGGCTCCT TGCAGCTGGA TCTCAACCGC
    ATGCCCAAGC CTGCCAAGAC AGCCGAGAAG TGCTCCCTGG ACCAGCTGGA TGACACTTTC
    CACCCAGAGT GGTTTGTGTC CCTTTTTGAA CAGAAAACTG TGAAGGGCTG GTGGCCCTGT
    GTGGCGGAAG AGGGTGAGAA GAAGAAGCTG GCGGGCAAGC TGGAGATGAC CTTGGAGATT
    GTGGCGGAGA GTGAGCATGA GGAGCGGCCC GCTGGCCAGG GCCGGGATGA GCCCAACATG
    AACCCCAAGC TCGAGGACCC AAGGCGCCCT GACACCTCCT TCCTCTGGTT CACCTCCCCG
    TACAAGACTA TGAAGTTCAT CCTGTGGCGG CGTTTCCGGT GTGCCATCAT CCTCTTCATC
    ATCCTCTTCA TCCTACTGCT GTTCTTGGGC ATCTTCGTTT ACGCCTTCCC GAACTATGCT
    GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023474675.1
    CDS211..6660
    Translation

    Target ORF information:

    RefSeq Version XM_023618907.1
    Organism Equus caballus(horse)
    Definition Equus caballus dysferlin (DYSF), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023618907.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGTGCT GCCTGCTAGT GAAGGCCAGC AACCTTCCCA CTGTGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAA
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG GGTCCCCCTG
    GACCAGAGCT CTGAGCTTCA CGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
    TTCCTGGGAG AAGCCAAGGT CCCACTCCGG GAGGTCCTTG CCACCCCCAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACGCCTCT CCCTGGAGCT GTGCCCCTGT TCCCACCCTC TGTGCCGCTG
    GAACCCTCCC CGACTCTGCC CGATGTGGAT ATGCTGGCTG GTGGGGGACA GAGCCGGGCC
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAATGG
    CAGGCCCCCA CGGATACAGG AGGAGAGGAG GACACCGAGG ACCAGGGGCT CACGGGAGAT
    GAGGCAGAGC CATTCTTGGA TCAGAACGGA GCCCCAGGCC CCGGGGCTCC CACCACCCTG
    AAGAAGCCAC CTTCCCATCC TCCCCCCTAC CATCCTGGGG GGAAAAGGAA GAGAAGCACG
    CCTGCGCCCA GAAAGCTGCT TTCGGATAAA CCACAGGACT TCCAGATCAG GGTCCAGGTG
    ATCGAGGGGC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCGGCC
    AGGCAGACCA AGCGGACTCG GATTCACAGG GGAAACAGCC CGCTCTTCAA CGAGACTCTC
    TTCTTCAACG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGGCCGTCTT TATCACGGTG
    GTAGACTCCT GTTCGCTCCG GACAGATGCC CTCATCGGGG AGTTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCT
    GACGATTTCT CTGCTGGGCC CAAAGGCTAC CTGAAAGCAA GCCTTTGTGT GCTGGGGCCT
    GGAGACGAAG CTCCGCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAGC
    AATCTGCTCA GGCCAACTGG CATGGCCCTT CGAGGAGCGC ACTTCTGTCT GAAGGTCTTC
    AGGGCTGAGG ACTTACCACA GATGGACGAT GCCGTGGTGG ACAGCGTGAA GCAGATCTTC
    GGCTTTGACA GTAACAAGAA GAACCTGGTG GATCCCTTCG TCGAGGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ATGGCCAACC CTCAGTGGAA CCAGAGCATC
    ACGCTGCCTG TCATGTTTCC CTCCATGTCT GAAAAAATGA GGATTCGTGT CATAGACTGG
    GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAATATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGC AGAGGAGCCT GCAGGTGTGG TCAAGCCTCC TCCAGCCACG
    GACCTGGACG ACCACCTGGG CTTCCTCCCC ACCTTCGGGC CCTGCTATGT CAACCTCTAC
    GGCAGCCCCA GGGAGTTCAC TGGCTTCCCA GACCCCTATG CAGAGCTCAA CACGGGCAAG
    GGAGAAGGCG TGGCCTACCG AGGCCGGCTT CTGGTCTCCC TGGAGACCAA ACTGGTGGAG
    CAAGGCGAGC AGAAGGTGGA GGATCTCGCT GCAGATGACA TCCTTCGGGT GGAGAAGTAC
    CTCCGGAGGC GCAAGTACTC CCTCTTTGCT GCCTTCTACT CGGCCTGCAT GCTGCAGGAT
    GTCGACGATG CCATCCAGTT CGAGGTCAGC ATCGGAAACT ACGGGAACAA GTTTGACACG
    ACCTGCCTGC CGCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA CGGGTGCCAC
    TACTACTACT TACCCTGGGG CAACGTGAAG CCTGTGGTGG TGCTGTCATC CTACTGGGAA
    GACATCAGCC ACAGAGTCGA CACTCAGAAC CAGCTGCTCC GGATCGCCGA CCGGCTGGAA
    GCCGGCCTGG AGCAGGTCCA CCTGGCCCTG AAGGCGCGGT GCTCCACCGA GGATGTGGAC
    TCCCTGGTGG CTCAGCTGAT GGATGAGCTC ATTGCAGACT GCAGCCAGCC CCTGTGCAAC
    GTCCAAGAGA CGCCCTCTGC CACCCACCTG GACCAGTACC TGTACCGGCT GCGCACCCGT
    CACCTGAGCC AGATCAGTGA GGCGGCCCTG GCCTTGAAGC TCGGCCACAG TGAGCTCCCC
    GCCGCCCTTG AGCAGGCTGA GGACTGGCTC CTGCGTCTCC GTGCCCTGGC GGAGGAGCCC
    CAGAACAGCC TGCCTGACGT GGTCATCTGG ATGCTGCAGG GAGACAAGCG GGTGGCGTAC
    CAGCGGGTGC CCGCCCACGA GGTCCTCTTC TCCCGGCGGG GCGCCAACTA CTGTGGCAAG
    AATTGTGGGA AGCTGCAGAC GATCTTTCTG AAGTATCCAA TGGAAGGGGT GCCGGGAGCC
    CGGATGCCGG TGCAGATACG GGTGAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAG
    TTCAATCAGT TTGCTGAGGG CAAGCTCTCT GTCTTCGCTG AAACCTACGA GAACCAGACC
    AAGCTGGCCC TAGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAC
    GTCACAGGCA AGATCAAGCT ACCCAAGGAC AGCTTCCGCC CCTCGGCCGG CTGGGCCTGG
    GCTGGAGAGT GGTTCGTGTG TCCGGAGAAG ACCCTACTCC ACGACACCGA TGCGGGCCAC
    CTGAGCTTTG TGGAGGAGGT GTTTGAGAAC CAGACCCGGC TGCCCGGAGG CCAGTGGATC
    TACATGAGTG ACAACTACAC AGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC
    GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCC
    GTTGACGAGC AAGGCTGGGA GTACAGCATC ACCATCCCTC CGGAACGGAA GCCAAGGCAC
    TGGGTCCCTG CCGAGAAGAT GTACTACACA CACCGTCGGC GGCGCTGGGT CCGCCTGCGC
    AGGAGGGACC TCAGCCAGAT GGAGGCGCTG AAGAGGCACA GGCAGGCGGA GGCGGAGGGC
    GAGGGCTGGG AGTACGCCTC GCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACC
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CACTGGAGAA GACAGGGCCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCGTGG TGGACGACAG GAGTGAAGAT
    TCGGTGTCCG TCTCCACCTT GAGCTTCGGC GTGAACAGAC CCACGATTTC CTGCATCTTC
    GACTACGGGA ACCGCTACCA TCTACGCTGC TACATGTACC AGGCCCGGGA CCTGCCCGCC
    ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCAATCGTCT CCTTCCTGCA CCAGAGCCAG
    AAGACAGTGG TGGTGAAGAA CACCCTGAAC CCCACCTGGG ACCAGACACT CATCTTCTAT
    GAGATCGAGA TCTTTGGTGA GCCGCCCAGC ATCGCCGAGC AGCCGCCCAG CATCGTGGTG
    GAGCTATACG ACCATGACAC CTACGGTGCA GATGAGTTTA TGGGACGCTG CATCTGTCAG
    CCGACTCTGG AACGGATGCC CCGGCTGACC TGGTTCCCAC TGACTAGGGG CAGCCAGCCG
    GCGGGCGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCGGC CATCTACCAC
    ATTCCTGGTT TTGAGGTGCA GGATACATCA GGGATCCTGG AAGAGCTGTG TGCACCTGCC
    TTCTTCCTCC AGGGCCTCCT CCAAACGTCG GAGGACACAG ACCTGCCCTA CCCGCCACCC
    CAGAGAGAGG CCAACATCTA CATGGTTCCC CAGAACATCA AGCCTGCTCT CCAGCGGACG
    GCCATCGAGA TCCTGGCATG GGGCCTGCGG AACATGAAGA GTTACCACCT GGCCAGTGTC
    TCCTCCCCCA GCCTCGTGGT GGAGTGCGGG GGCCAGACGG TGCAGTCCTG TGTCATCAGG
    AACCTCCAGA AGAACCCCAA CTTCGACGTC TGCACCCTCT TCATGGAAGT GATGCTGCCC
    AGGGAGGAGC TCTACTGTCC CCCCATCGTG GTCAAGGTCA TCGATAACTG CCAGTTTGGC
    CGCCGGCCTG TGGTGGGCCA GTGTACCATC TGCTCCCTGG AGAGCTTCCT GTGTGACCCC
    TACTCAGAGG AGAGTCCGTC CCCACAGGGC GGCCCAGATG ATGTGAGCTT ACTCAGTCCT
    GGGGAAGATG TGCTCATTGA CATTGATGAC AAGGAGCCCC TCATCCCTAT GCAGCTTGCA
    GATGGTCTGT CGGGCTCGGC CCCCACTAAC ATGGCATCTT CTCCGTCCAG TCCTCATGAG
    GAGGAGTTCA TCGATTGGTG GAGCAAATTC TTTGCCTCGA TAGGGGAGAG GGAAAAGTGC
    GGGTCCTACT TGGAGAAGGA CTTTGACACT CTAAAGGTCT ATGACACACA ACTGGAGAAT
    GTGGAGGCCT TTGAGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG
    ACTCAGGAGG AGATGGAAGA CCCATCTGTC GTTGGGGAAT TTAAGGGCCT CTTCAAAATT
    TATCCCCTCC CAGAAGACCC GGCCATCCCC ATGCCCCCAA GACAGTTCCA CCAGCTAGCT
    GCCCAGGGAC CCCAGGAGTG CCTGGTCCGC ATCTACATCA TCCGGGCATT CGGCCTGCAG
    CCCAAGGACC CCAATGGGAA GTGTGACCCT TACATCAAGA TCTCCATAGG GAAGAAATCC
    GTGAGCGACC AGGATAACTA CATCCCCTGC ACTCTGGAGC CTGTGTTTGG AAAGATGTTC
    GAACTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACTCTCTA TGACTATGAC
    CTCCTCTCCA AGGACGAGAT GATCGGGGAG ACGGTCATCG ACCTGGAGAA TAGGCTGCTG
    TCCAAGTTTG GAGCTCGCTG CGGACTCCCA CAGACCTACT GTGTCTCTGG ACCGAACCAG
    TGGAGGGACC AGCTCCGCCC CTCCCAGCTT CTCCACCTGT TCTGCCAGCA GCAGAGAGTC
    AAGGCCCCCG TGTACCAGAC CGACCGTGTG GTGTTTGGGA ATAAAGAATA CACGATTGAA
    GAAATAGAGG CTGGGAGGGT CCTGAATCCA CACCTGGGCC CAGTGGAAGA GCGCCTGGCC
    TTGCACGTCC TCCAGCAGCA GGGCTTGGTG CCCGAGCATG TGGAGTCCCG GCCCCTCTAT
    AGCCCCCTGC AGCCGGACAT TGAGCAGGGG AAGCTGCAGA TGTGGATCGA CCTGTTTCCA
    AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC AACATCACTC CACGGAGAGC CAGAAGGTTT
    TTCCTGCGTT GCATTATCTG GAACACCAAG GATGTGATCC TGGACGACCT CAGCATCACA
    GGAGAGAAGA TGAGCGACAT TTATGTGAAA GGTTGGATGG TTGGCTTTGA AGAACACAAG
    CAAAAGACAG ATGTGCATTA TCGGTCCCTG GGAGGCGAAG GGAACTTTAA CTGGAGGTTC
    ATTTTCCCCT TTGACTACCT GCTGGCTGAG CAAGTCTGCA CCGTCTCCAA GAAGGATGCT
    TTCTGGAAGC TGGACAAGAC CGAGAGCAAA ATCCCAGCAC GAGTGGTATT CCAGATCTGG
    GACAATGACA AGTTCTCCTT TGATGATTTC CTGGGCTCCT TGCAGCTGGA TCTCAACCGC
    ATGCCCAAGC CTGCCAAGAC AGCCGAGAAG TGCTCCCTGG ACCAGCTGGA TGACACTTTC
    CACCCAGAGT GGTTTGTGTC CCTTTTTGAA CAGAAAACTG TGAAGGGCTG GTGGCCCTGT
    GTGGCGGAAG AGGGTGAGAA GAAGAAGCTG GCGGGCAAGC TGGAGATGAC CTTGGAGATT
    GTGGCGGAGA GTGAGCATGA GGAGCGGCCC GCTGGCCAGG GCCGGGATGA GCCCAACATG
    AACCCCAAGC TCGAGGACCC AAGGCGCCCT GACACCTCCT TCCTCTGGTT CACCTCCCCG
    TACAAGACTA TGAAGTTCAT CCTGTGGCGG CGTTTCCGGT GTGCCATCAT CCTCTTCATC
    ATCCTCTTCA TCCTACTGCT GTTCTTGGGC ATCTTCGTTT ACGCCTTCCC GAACTATGCT
    GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb49776
    Clone ID Related Accession (Same CDS sequence) XM_023618908.1
    Accession Version XM_023618908.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6408bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product dysferlin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009158.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGTGCT GCCTGCTAGT GAAGGCCAGC AACCTTCCCA CTGTGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAA
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG GGTCCCCCTG
    GACCAGAGCT CTGAGCTTCA CGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
    TTCCTGGGAG AAGCCAAGGT CCCACTCCGG GAGGTCCTTG CCACCCCCAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACGCCTCT CCCTGGAGCT GTGCCCCTGT TCCCACCCTC TGTGCCGCTG
    GAACCCTCCC CGACTCTGCC CGATGTGGAT ATGCTGGCTG GTGGGGGACA GAGCCGGGCC
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAATGG
    CAGGCCCCCA CGGATACAGG AGGAGAGGAG GACACCGAGG ACCAGGGGCT CACGGGAGAT
    GAGGCAGAGC CATTCTTGGA TCAGAACGGA GCCCCAGGCC CCGGGGCTCC CACCACCCTG
    AAGAAGCCAC CTTCCCATCC TCCCCCCTAC CATCCTGGGG GGAAAAGGAA GAGAAGCACG
    CCTGCGCCCA GAAAGCTGCT TTCGGATAAA CCACAGGACT TCCAGATCAG GGTCCAGGTG
    ATCGAGGGGC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCGGCC
    AGGCAGACCA AGCGGACTCG GATTCACAGG GGAAACAGCC CGCTCTTCAA CGAGACTCTC
    TTCTTCAACG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGGCCGTCTT TATCACGGTG
    GTAGACTCCT GTTCGCTCCG GACAGATGCC CTCATCGGGG AGTTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCT
    GACGATTTCT CTGCTGGGCC CAAAGGCTAC CTGAAAGCAA GCCTTTGTGT GCTGGGGCCT
    GGAGACGAAG CTCCGCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAGC
    AATCTGCTCA GGCCAACTGG CATGGCCCTT CGAGGAGCGC ACTTCTGTCT GAAGGTCTTC
    AGGGCTGAGG ACTTACCACA GATGGACGAT GCCGTGGTGG ACAGCGTGAA GCAGATCTTC
    GGCTTTGACA GTAACAAGAA GAACCTGGTG GATCCCTTCG TCGAGGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ATGGCCAACC CTCAGTGGAA CCAGAGCATC
    ACGCTGCCTG TCATGTTTCC CTCCATGTCT GAAAAAATGA GGATTCGTGT CATAGACTGG
    GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAATATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGC AGAGGAGCCT GCAGGTGTGG TCAAGCCTCC TCCAGCCACG
    GACCTGGACG ACCACCTGGG CTTCCTCCCC ACCTTCGGGC CCTGCTATGT CAACCTCTAC
    GGCAGCCCCA GGGAGTTCAC TGGCTTCCCA GACCCCTATG CAGAGCTCAA CACGGGCAAG
    GGAGAAGGCG TGGCCTACCG AGGCCGGCTT CTGGTCTCCC TGGAGACCAA ACTGGTGGAG
    CAAGGCGAGC AGAAGGTGGA GGATCTCGCT GCAGATGACA TCCTTCGGGT GGAGAAGTAC
    CTCCGGAGGC GCAAGTACTC CCTCTTTGCT GCCTTCTACT CGGCCTGCAT GCTGCAGGAT
    GTCGACGATG CCATCCAGTT CGAGGTCAGC ATCGGAAACT ACGGGAACAA GTTTGACACG
    ACCTGCCTGC CGCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA CGGGTGCCAC
    TACTACTACT TACCCTGGGG CAACGTGAAG CCTGTGGTGG TGCTGTCATC CTACTGGGAA
    GACATCAGCC ACAGAGTCGA CACTCAGAAC CAGCTGCTCC GGATCGCCGA CCGGCTGGAA
    GCCGGCCTGG AGCAGGTCCA CCTGGCCCTG AAGGCGCGGT GCTCCACCGA GGATGTGGAC
    TCCCTGGTGG CTCAGCTGAT GGATGAGCTC ATTGCAGACT GCAGCCAGCC CCTGTGCAAC
    GTCCAAGAGA CGCCCTCTGC CACCCACCTG GACCAGTACC TGTACCGGCT GCGCACCCGT
    CACCTGAGCC AGATCAGTGA GGCGGCCCTG GCCTTGAAGC TCGGCCACAG TGAGCTCCCC
    GCCGCCCTTG AGCAGGCTGA GGACTGGCTC CTGCGTCTCC GTGCCCTGGC GGAGGAGCCC
    CAGAACAGCC TGCCTGACGT GGTCATCTGG ATGCTGCAGG GAGACAAGCG GGTGGCGTAC
    CAGCGGGTGC CCGCCCACGA GGTCCTCTTC TCCCGGCGGG GCGCCAACTA CTGTGGCAAG
    AATTGTGGGA AGCTGCAGAC GATCTTTCTG AAGTATCCAA TGGAAGGGGT GCCGGGAGCC
    CGGATGCCGG TGCAGATACG GGTGAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAG
    TTCAATCAGT TTGCTGAGGG CAAGCTCTCT GTCTTCGCTG AAACCTACGA GAACCAGACC
    AAGCTGGCCC TAGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAC
    GTCACAGGCA AGATCAAGCT ACCCAAGGAC AGCTTCCGCC CCTCGGCCGG CTGGGCCTGG
    GCTGGAGAGT GGTTCGTGTG TCCGGAGAAG ACCCTACTCC ACGACACCGA TGCGGGCCAC
    CTGAGCTTTG TGGAGGAGGT GTTTGAGAAC CAGACCCGGC TGCCCGGAGG CCAGTGGATC
    TACATGAGTG ACAACTACAC AGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC
    GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCC
    GTTGACGAGC AAGGCTGGGA GTACAGCATC ACCATCCCTC CGGAACGGAA GCCAAGGCAC
    TGGGTCCCTG CCGAGAAGAT GTACTACACA CACCGTCGGC GGCGCTGGGT CCGCCTGCGC
    AGGAGGGACC TCAGCCAGAT GGAGGCGCTG AAGAGGCACA GGCAGGCGGA GGCGGAGGGC
    GAGGGCTGGG AGTACGCCTC GCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACC
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CACTGGAGAA GACAGGGCCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCGTGG TGGACGACAG GAGTGAAGAT
    TCGGTGTCCG TCTCCACCTT GAGCTTCGGC GTGAACAGAC CCACGATTTC CTGCATCTTC
    GACTACGGGA ACCGCTACCA TCTACGCTGC TACATGTACC AGGCCCGGGA CCTGCCCGCC
    ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCAATCGTCT CCTTCCTGCA CCAGAGCCAG
    AAGACAGTGG TGGTGAAGAA CACCCTGAAC CCCACCTGGG ACCAGACACT CATCTTCTAT
    GAGATCGAGA TCTTTGGTGA GCCGCCCAGC ATCGCCGAGC AGCCGCCCAG CATCGTGGTG
    GAGCTATACG ACCATGACAC CTACGGTGCA GATGAGTTTA TGGGACGCTG CATCTGTCAG
    CCGACTCTGG AACGGATGCC CCGGCTGACC TGGTTCCCAC TGACTAGGGG CAGCCAGCCG
    GCGGGCGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCGGC CATCTACCAC
    ATTCCTGGTT TTGAGGTGCA GGATACATCA GGGATCCTGG AAGAGTCGGA GGACACAGAC
    CTGCCCTACC CGCCACCCCA GAGAGAGGCC AACATCTACA TGGTTCCCCA GAACATCAAG
    CCTGCTCTCC AGCGGACGGC CATCGAGATC CTGGCATGGG GCCTGCGGAA CATGAAGAGT
    TACCACCTGG CCAGTGTCTC CTCCCCCAGC CTCGTGGTGG AGTGCGGGGG CCAGACGGTG
    CAGTCCTGTG TCATCAGGAA CCTCCAGAAG AACCCCAACT TCGACGTCTG CACCCTCTTC
    ATGGAAGTGA TGCTGCCCAG GGAGGAGCTC TACTGTCCCC CCATCGTGGT CAAGGTCATC
    GATAACTGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCTG CTCCCTGGAG
    AGCTTCCTGT GTGACCCCTA CTCAGAGGAG AGTCCGTCCC CACAGGGCGG CCCAGATGAT
    GTGAGCTTAC TCAGTCCTGG GGAAGATGTG CTCATTGACA TTGATGACAA GGAGCCCCTC
    ATCCCTATGC AGCTTGCAGA TGGTCTGTCG GGCTCGGCCC CCACTAACAT GGCATCTTCT
    CCGTCCAGTC CTCATGAGGA GGAGTTCATC GATTGGTGGA GCAAATTCTT TGCCTCGATA
    GGGGAGAGGG AAAAGTGCGG GTCCTACTTG GAGAAGGACT TTGACACTCT AAAGGTCTAT
    GACACACAAC TGGAGAATGT GGAGGCCTTT GAGGGCCTGT CTGACTTTTG TAACACCTTC
    AAGCTCTACC GGGGCAAGAC TCAGGAGGAG ATGGAAGACC CATCTGTCGT TGGGGAATTT
    AAGGGCCTCT TCAAAATTTA TCCCCTCCCA GAAGACCCGG CCATCCCCAT GCCCCCAAGA
    CAGTTCCACC AGCTAGCTGC CCAGGGACCC CAGGAGTGCC TGGTCCGCAT CTACATCATC
    CGGGCATTCG GCCTGCAGCC CAAGGACCCC AATGGGAAGT GTGACCCTTA CATCAAGATC
    TCCATAGGGA AGAAATCCGT GAGCGACCAG GATAACTACA TCCCCTGCAC TCTGGAGCCT
    GTGTTTGGAA AGATGTTCGA ACTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTGAAGATC
    ACTCTCTATG ACTATGACCT CCTCTCCAAG GACGAGATGA TCGGGGAGAC GGTCATCGAC
    CTGGAGAATA GGCTGCTGTC CAAGTTTGGA GCTCGCTGCG GACTCCCACA GACCTACTGT
    GTCTCTGGAC CGAACCAGTG GAGGGACCAG CTCCGCCCCT CCCAGCTTCT CCACCTGTTC
    TGCCAGCAGC AGAGAGTCAA GGCCCCCGTG TACCAGACCG ACCGTGTGGT GTTTGGGAAT
    AAAGAATACA CGATTGAAGA AATAGAGGCT GGGAGGGTCC TGAATCCACA CCTGGGCCCA
    GTGGAAGAGC GCCTGGCCTT GCACGTCCTC CAGCAGCAGG GCTTGGTGCC CGAGCATGTG
    GAGTCCCGGC CCCTCTATAG CCCCCTGCAG CCGGACATTG AGCAGGGGAA GCTGCAGATG
    TGGATCGACC TGTTTCCAAA GGCCCTGGGG CGGCCTGGAC CTCCCTTCAA CATCACTCCA
    CGGAGAGCCA GAAGGTTTTT CCTGCGTTGC ATTATCTGGA ACACCAAGGA TGTGATCCTG
    GACGACCTCA GCATCACAGG AGAGAAGATG AGCGACATTT ATGTGAAAGG TTGGATGGTT
    GGCTTTGAAG AACACAAGCA AAAGACAGAT GTGCATTATC GGTCCCTGGG AGGCGAAGGG
    AACTTTAACT GGAGGTTCAT TTTCCCCTTT GACTACCTGC TGGCTGAGCA AGTCTGCACC
    GTCTCCAAGA AGGATGCTTT CTGGAAGCTG GACAAGACCG AGAGCAAAAT CCCAGCACGA
    GTGGTATTCC AGATCTGGGA CAATGACAAG TTCTCCTTTG ATGATTTCCT GGGCTCCTTG
    CAGCTGGATC TCAACCGCAT GCCCAAGCCT GCCAAGACAG CCGAGAAGTG CTCCCTGGAC
    CAGCTGGATG ACACTTTCCA CCCAGAGTGG TTTGTGTCCC TTTTTGAACA GAAAACTGTG
    AAGGGCTGGT GGCCCTGTGT GGCGGAAGAG GGTGAGAAGA AGAAGCTGGC GGGCAAGCTG
    GAGATGACCT TGGAGATTGT GGCGGAGAGT GAGCATGAGG AGCGGCCCGC TGGCCAGGGC
    CGGGATGAGC CCAACATGAA CCCCAAGCTC GAGGACCCAA GGCGCCCTGA CACCTCCTTC
    CTCTGGTTCA CCTCCCCGTA CAAGACTATG AAGTTCATCC TGTGGCGGCG TTTCCGGTGT
    GCCATCATCC TCTTCATCAT CCTCTTCATC CTACTGCTGT TCTTGGGCAT CTTCGTTTAC
    GCCTTCCCGA ACTATGCTGC CATGAAGCTG GTGAAGCCCT TCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023474676.1
    CDS211..6618
    Translation

    Target ORF information:

    RefSeq Version XM_023618908.1
    Organism Equus caballus(horse)
    Definition Equus caballus dysferlin (DYSF), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023618908.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGTGCT GCCTGCTAGT GAAGGCCAGC AACCTTCCCA CTGTGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAA
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG GGTCCCCCTG
    GACCAGAGCT CTGAGCTTCA CGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
    TTCCTGGGAG AAGCCAAGGT CCCACTCCGG GAGGTCCTTG CCACCCCCAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACGCCTCT CCCTGGAGCT GTGCCCCTGT TCCCACCCTC TGTGCCGCTG
    GAACCCTCCC CGACTCTGCC CGATGTGGAT ATGCTGGCTG GTGGGGGACA GAGCCGGGCC
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAATGG
    CAGGCCCCCA CGGATACAGG AGGAGAGGAG GACACCGAGG ACCAGGGGCT CACGGGAGAT
    GAGGCAGAGC CATTCTTGGA TCAGAACGGA GCCCCAGGCC CCGGGGCTCC CACCACCCTG
    AAGAAGCCAC CTTCCCATCC TCCCCCCTAC CATCCTGGGG GGAAAAGGAA GAGAAGCACG
    CCTGCGCCCA GAAAGCTGCT TTCGGATAAA CCACAGGACT TCCAGATCAG GGTCCAGGTG
    ATCGAGGGGC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCGGCC
    AGGCAGACCA AGCGGACTCG GATTCACAGG GGAAACAGCC CGCTCTTCAA CGAGACTCTC
    TTCTTCAACG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGGCCGTCTT TATCACGGTG
    GTAGACTCCT GTTCGCTCCG GACAGATGCC CTCATCGGGG AGTTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCT
    GACGATTTCT CTGCTGGGCC CAAAGGCTAC CTGAAAGCAA GCCTTTGTGT GCTGGGGCCT
    GGAGACGAAG CTCCGCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAGC
    AATCTGCTCA GGCCAACTGG CATGGCCCTT CGAGGAGCGC ACTTCTGTCT GAAGGTCTTC
    AGGGCTGAGG ACTTACCACA GATGGACGAT GCCGTGGTGG ACAGCGTGAA GCAGATCTTC
    GGCTTTGACA GTAACAAGAA GAACCTGGTG GATCCCTTCG TCGAGGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ATGGCCAACC CTCAGTGGAA CCAGAGCATC
    ACGCTGCCTG TCATGTTTCC CTCCATGTCT GAAAAAATGA GGATTCGTGT CATAGACTGG
    GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAATATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGC AGAGGAGCCT GCAGGTGTGG TCAAGCCTCC TCCAGCCACG
    GACCTGGACG ACCACCTGGG CTTCCTCCCC ACCTTCGGGC CCTGCTATGT CAACCTCTAC
    GGCAGCCCCA GGGAGTTCAC TGGCTTCCCA GACCCCTATG CAGAGCTCAA CACGGGCAAG
    GGAGAAGGCG TGGCCTACCG AGGCCGGCTT CTGGTCTCCC TGGAGACCAA ACTGGTGGAG
    CAAGGCGAGC AGAAGGTGGA GGATCTCGCT GCAGATGACA TCCTTCGGGT GGAGAAGTAC
    CTCCGGAGGC GCAAGTACTC CCTCTTTGCT GCCTTCTACT CGGCCTGCAT GCTGCAGGAT
    GTCGACGATG CCATCCAGTT CGAGGTCAGC ATCGGAAACT ACGGGAACAA GTTTGACACG
    ACCTGCCTGC CGCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA CGGGTGCCAC
    TACTACTACT TACCCTGGGG CAACGTGAAG CCTGTGGTGG TGCTGTCATC CTACTGGGAA
    GACATCAGCC ACAGAGTCGA CACTCAGAAC CAGCTGCTCC GGATCGCCGA CCGGCTGGAA
    GCCGGCCTGG AGCAGGTCCA CCTGGCCCTG AAGGCGCGGT GCTCCACCGA GGATGTGGAC
    TCCCTGGTGG CTCAGCTGAT GGATGAGCTC ATTGCAGACT GCAGCCAGCC CCTGTGCAAC
    GTCCAAGAGA CGCCCTCTGC CACCCACCTG GACCAGTACC TGTACCGGCT GCGCACCCGT
    CACCTGAGCC AGATCAGTGA GGCGGCCCTG GCCTTGAAGC TCGGCCACAG TGAGCTCCCC
    GCCGCCCTTG AGCAGGCTGA GGACTGGCTC CTGCGTCTCC GTGCCCTGGC GGAGGAGCCC
    CAGAACAGCC TGCCTGACGT GGTCATCTGG ATGCTGCAGG GAGACAAGCG GGTGGCGTAC
    CAGCGGGTGC CCGCCCACGA GGTCCTCTTC TCCCGGCGGG GCGCCAACTA CTGTGGCAAG
    AATTGTGGGA AGCTGCAGAC GATCTTTCTG AAGTATCCAA TGGAAGGGGT GCCGGGAGCC
    CGGATGCCGG TGCAGATACG GGTGAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAG
    TTCAATCAGT TTGCTGAGGG CAAGCTCTCT GTCTTCGCTG AAACCTACGA GAACCAGACC
    AAGCTGGCCC TAGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAC
    GTCACAGGCA AGATCAAGCT ACCCAAGGAC AGCTTCCGCC CCTCGGCCGG CTGGGCCTGG
    GCTGGAGAGT GGTTCGTGTG TCCGGAGAAG ACCCTACTCC ACGACACCGA TGCGGGCCAC
    CTGAGCTTTG TGGAGGAGGT GTTTGAGAAC CAGACCCGGC TGCCCGGAGG CCAGTGGATC
    TACATGAGTG ACAACTACAC AGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC
    GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCC
    GTTGACGAGC AAGGCTGGGA GTACAGCATC ACCATCCCTC CGGAACGGAA GCCAAGGCAC
    TGGGTCCCTG CCGAGAAGAT GTACTACACA CACCGTCGGC GGCGCTGGGT CCGCCTGCGC
    AGGAGGGACC TCAGCCAGAT GGAGGCGCTG AAGAGGCACA GGCAGGCGGA GGCGGAGGGC
    GAGGGCTGGG AGTACGCCTC GCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACC
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CACTGGAGAA GACAGGGCCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCGTGG TGGACGACAG GAGTGAAGAT
    TCGGTGTCCG TCTCCACCTT GAGCTTCGGC GTGAACAGAC CCACGATTTC CTGCATCTTC
    GACTACGGGA ACCGCTACCA TCTACGCTGC TACATGTACC AGGCCCGGGA CCTGCCCGCC
    ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCAATCGTCT CCTTCCTGCA CCAGAGCCAG
    AAGACAGTGG TGGTGAAGAA CACCCTGAAC CCCACCTGGG ACCAGACACT CATCTTCTAT
    GAGATCGAGA TCTTTGGTGA GCCGCCCAGC ATCGCCGAGC AGCCGCCCAG CATCGTGGTG
    GAGCTATACG ACCATGACAC CTACGGTGCA GATGAGTTTA TGGGACGCTG CATCTGTCAG
    CCGACTCTGG AACGGATGCC CCGGCTGACC TGGTTCCCAC TGACTAGGGG CAGCCAGCCG
    GCGGGCGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCGGC CATCTACCAC
    ATTCCTGGTT TTGAGGTGCA GGATACATCA GGGATCCTGG AAGAGTCGGA GGACACAGAC
    CTGCCCTACC CGCCACCCCA GAGAGAGGCC AACATCTACA TGGTTCCCCA GAACATCAAG
    CCTGCTCTCC AGCGGACGGC CATCGAGATC CTGGCATGGG GCCTGCGGAA CATGAAGAGT
    TACCACCTGG CCAGTGTCTC CTCCCCCAGC CTCGTGGTGG AGTGCGGGGG CCAGACGGTG
    CAGTCCTGTG TCATCAGGAA CCTCCAGAAG AACCCCAACT TCGACGTCTG CACCCTCTTC
    ATGGAAGTGA TGCTGCCCAG GGAGGAGCTC TACTGTCCCC CCATCGTGGT CAAGGTCATC
    GATAACTGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCTG CTCCCTGGAG
    AGCTTCCTGT GTGACCCCTA CTCAGAGGAG AGTCCGTCCC CACAGGGCGG CCCAGATGAT
    GTGAGCTTAC TCAGTCCTGG GGAAGATGTG CTCATTGACA TTGATGACAA GGAGCCCCTC
    ATCCCTATGC AGCTTGCAGA TGGTCTGTCG GGCTCGGCCC CCACTAACAT GGCATCTTCT
    CCGTCCAGTC CTCATGAGGA GGAGTTCATC GATTGGTGGA GCAAATTCTT TGCCTCGATA
    GGGGAGAGGG AAAAGTGCGG GTCCTACTTG GAGAAGGACT TTGACACTCT AAAGGTCTAT
    GACACACAAC TGGAGAATGT GGAGGCCTTT GAGGGCCTGT CTGACTTTTG TAACACCTTC
    AAGCTCTACC GGGGCAAGAC TCAGGAGGAG ATGGAAGACC CATCTGTCGT TGGGGAATTT
    AAGGGCCTCT TCAAAATTTA TCCCCTCCCA GAAGACCCGG CCATCCCCAT GCCCCCAAGA
    CAGTTCCACC AGCTAGCTGC CCAGGGACCC CAGGAGTGCC TGGTCCGCAT CTACATCATC
    CGGGCATTCG GCCTGCAGCC CAAGGACCCC AATGGGAAGT GTGACCCTTA CATCAAGATC
    TCCATAGGGA AGAAATCCGT GAGCGACCAG GATAACTACA TCCCCTGCAC TCTGGAGCCT
    GTGTTTGGAA AGATGTTCGA ACTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTGAAGATC
    ACTCTCTATG ACTATGACCT CCTCTCCAAG GACGAGATGA TCGGGGAGAC GGTCATCGAC
    CTGGAGAATA GGCTGCTGTC CAAGTTTGGA GCTCGCTGCG GACTCCCACA GACCTACTGT
    GTCTCTGGAC CGAACCAGTG GAGGGACCAG CTCCGCCCCT CCCAGCTTCT CCACCTGTTC
    TGCCAGCAGC AGAGAGTCAA GGCCCCCGTG TACCAGACCG ACCGTGTGGT GTTTGGGAAT
    AAAGAATACA CGATTGAAGA AATAGAGGCT GGGAGGGTCC TGAATCCACA CCTGGGCCCA
    GTGGAAGAGC GCCTGGCCTT GCACGTCCTC CAGCAGCAGG GCTTGGTGCC CGAGCATGTG
    GAGTCCCGGC CCCTCTATAG CCCCCTGCAG CCGGACATTG AGCAGGGGAA GCTGCAGATG
    TGGATCGACC TGTTTCCAAA GGCCCTGGGG CGGCCTGGAC CTCCCTTCAA CATCACTCCA
    CGGAGAGCCA GAAGGTTTTT CCTGCGTTGC ATTATCTGGA ACACCAAGGA TGTGATCCTG
    GACGACCTCA GCATCACAGG AGAGAAGATG AGCGACATTT ATGTGAAAGG TTGGATGGTT
    GGCTTTGAAG AACACAAGCA AAAGACAGAT GTGCATTATC GGTCCCTGGG AGGCGAAGGG
    AACTTTAACT GGAGGTTCAT TTTCCCCTTT GACTACCTGC TGGCTGAGCA AGTCTGCACC
    GTCTCCAAGA AGGATGCTTT CTGGAAGCTG GACAAGACCG AGAGCAAAAT CCCAGCACGA
    GTGGTATTCC AGATCTGGGA CAATGACAAG TTCTCCTTTG ATGATTTCCT GGGCTCCTTG
    CAGCTGGATC TCAACCGCAT GCCCAAGCCT GCCAAGACAG CCGAGAAGTG CTCCCTGGAC
    CAGCTGGATG ACACTTTCCA CCCAGAGTGG TTTGTGTCCC TTTTTGAACA GAAAACTGTG
    AAGGGCTGGT GGCCCTGTGT GGCGGAAGAG GGTGAGAAGA AGAAGCTGGC GGGCAAGCTG
    GAGATGACCT TGGAGATTGT GGCGGAGAGT GAGCATGAGG AGCGGCCCGC TGGCCAGGGC
    CGGGATGAGC CCAACATGAA CCCCAAGCTC GAGGACCCAA GGCGCCCTGA CACCTCCTTC
    CTCTGGTTCA CCTCCCCGTA CAAGACTATG AAGTTCATCC TGTGGCGGCG TTTCCGGTGT
    GCCATCATCC TCTTCATCAT CCTCTTCATC CTACTGCTGT TCTTGGGCAT CTTCGTTTAC
    GCCTTCCCGA ACTATGCTGC CATGAAGCTG GTGAAGCCCT TCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb49777
    Clone ID Related Accession (Same CDS sequence) XM_023618909.1
    Accession Version XM_023618909.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6408bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product dysferlin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009158.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGTGCT GCCTGCTAGT GAAGGCCAGC AACCTTCCCA CTGTGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAA
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG GGTCCCCCTG
    GACCAGAGCT CTGAGCTTCA CGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
    TTCCTGGGAG AAGCCAAGGT CCCACTCCGG GAGGTCCTTG CCACCCCCAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACGCCTCT CCCTGGAGCT GTGCCCCTGT TCCCACCCTC TGTGCCGCTG
    GAACCCTCCC CGACTCTGCC CGATGTGGAT ATGCTGGCTG GTGGGGGACA GAGCCGGGCC
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAATGG
    CAGGCCCCCA CGGATACAGG AGGAGAGGAG GACACCGAGG ACCAGGGGCT CACGGGAGAT
    GAGGCAGAGC CATTCTTGGA TCAGAACGGA GCCCCAGGCC CCGGGGCTCC CACCACCCTG
    AAGAAGCCAC CTTCCCATCC TCCCCCCTAC CATCCTGGGG GGAAAAGGAA GAGAAGCACG
    CCTGCGCCCA GAAAGCTGCT TTCGGATAAA CCACAGGACT TCCAGATCAG GGTCCAGGTG
    ATCGAGGGGC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCGGCC
    AGGCAGACCA AGCGGACTCG GATTCACAGG GGAAACAGCC CGCTCTTCAA CGAGACTCTC
    TTCTTCAACG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGGCCGTCTT TATCACGGTG
    GTAGACTCCT GTTCGCTCCG GACAGATGCC CTCATCGGGG AGTTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCT
    GACGATTTCT CTGCTGGGCC CAAAGGCTAC CTGAAAGCAA GCCTTTGTGT GCTGGGGCCT
    GGAGACGAAG CTCCGCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAGC
    AATCTGCTCA GGCCAACTGG CATGGCCCTT CGAGGAGCGC ACTTCTGTCT GAAGGTCTTC
    AGGGCTGAGG ACTTACCACA GATGGACGAT GCCGTGGTGG ACAGCGTGAA GCAGATCTTC
    GGCTTTGACA GTAACAAGAA GAACCTGGTG GATCCCTTCG TCGAGGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ATGGCCAACC CTCAGTGGAA CCAGAGCATC
    ACGCTGCCTG TCATGTTTCC CTCCATGTCT GAAAAAATGA GGATTCGTGT CATAGACTGG
    GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAATATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGC AGTGGACGAC CACCTGGGCT TCCTCCCCAC CTTCGGGCCC
    TGCTATGTCA ACCTCTACGG CAGCCCCAGG GAGTTCACTG GCTTCCCAGA CCCCTATGCA
    GAGCTCAACA CGGGCAAGGG AGAAGGCGTG GCCTACCGAG GCCGGCTTCT GGTCTCCCTG
    GAGACCAAAC TGGTGGAGCA AGGCGAGCAG AAGGTGGAGG ATCTCGCTGC AGATGACATC
    CTTCGGGTGG AGAAGTACCT CCGGAGGCGC AAGTACTCCC TCTTTGCTGC CTTCTACTCG
    GCCTGCATGC TGCAGGATGT CGACGATGCC ATCCAGTTCG AGGTCAGCAT CGGAAACTAC
    GGGAACAAGT TTGACACGAC CTGCCTGCCG CTGGCCTCCA CCACCCAGTA CAGCCGGGCG
    GTCTTTGACG GGTGCCACTA CTACTACTTA CCCTGGGGCA ACGTGAAGCC TGTGGTGGTG
    CTGTCATCCT ACTGGGAAGA CATCAGCCAC AGAGTCGACA CTCAGAACCA GCTGCTCCGG
    ATCGCCGACC GGCTGGAAGC CGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCGGTGC
    TCCACCGAGG ATGTGGACTC CCTGGTGGCT CAGCTGATGG ATGAGCTCAT TGCAGACTGC
    AGCCAGCCCC TGTGCAACGT CCAAGAGACG CCCTCTGCCA CCCACCTGGA CCAGTACCTG
    TACCGGCTGC GCACCCGTCA CCTGAGCCAG ATCAGTGAGG CGGCCCTGGC CTTGAAGCTC
    GGCCACAGTG AGCTCCCCGC CGCCCTTGAG CAGGCTGAGG ACTGGCTCCT GCGTCTCCGT
    GCCCTGGCGG AGGAGCCCCA GAACAGCCTG CCTGACGTGG TCATCTGGAT GCTGCAGGGA
    GACAAGCGGG TGGCGTACCA GCGGGTGCCC GCCCACGAGG TCCTCTTCTC CCGGCGGGGC
    GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTGCAGACGA TCTTTCTGAA GTATCCAATG
    GAAGGGGTGC CGGGAGCCCG GATGCCGGTG CAGATACGGG TGAAGCTCTG GTTCGGGCTC
    TCTGTGGATG AGAAGGAGTT CAATCAGTTT GCTGAGGGCA AGCTCTCTGT CTTCGCTGAA
    ACCTACGAGA ACCAGACCAA GCTGGCCCTA GTCGGGAACT GGGGCACAAC GGGCCTCACC
    TACCCCAAGT TTTCTGACGT CACAGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC
    TCGGCCGGCT GGGCCTGGGC TGGAGAGTGG TTCGTGTGTC CGGAGAAGAC CCTACTCCAC
    GACACCGATG CGGGCCACCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTG
    CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACAG ATGTGAACGG GGAGAAGGTG
    CTTCCCAAGG ATGACATCGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA AGAATGGTCC
    ACAGACCTCA ATCGGGCCGT TGACGAGCAA GGCTGGGAGT ACAGCATCAC CATCCCTCCG
    GAACGGAAGC CAAGGCACTG GGTCCCTGCC GAGAAGATGT ACTACACACA CCGTCGGCGG
    CGCTGGGTCC GCCTGCGCAG GAGGGACCTC AGCCAGATGG AGGCGCTGAA GAGGCACAGG
    CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCGC TCTTCGGCTG GAAGTTCCAC
    CTGGAGTACC GCAAGACCGA TGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCA
    CTGGAGAAGA CAGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCCCTGGG CGGCGTGGTG
    GACGACAGGA GTGAAGATTC GGTGTCCGTC TCCACCTTGA GCTTCGGCGT GAACAGACCC
    ACGATTTCCT GCATCTTCGA CTACGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG
    GCCCGGGACC TGCCCGCCAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC AATCGTCTCC
    TTCCTGCACC AGAGCCAGAA GACAGTGGTG GTGAAGAACA CCCTGAACCC CACCTGGGAC
    CAGACACTCA TCTTCTATGA GATCGAGATC TTTGGTGAGC CGCCCAGCAT CGCCGAGCAG
    CCGCCCAGCA TCGTGGTGGA GCTATACGAC CATGACACCT ACGGTGCAGA TGAGTTTATG
    GGACGCTGCA TCTGTCAGCC GACTCTGGAA CGGATGCCCC GGCTGACCTG GTTCCCACTG
    ACTAGGGGCA GCCAGCCGGC GGGCGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG
    AAGCCGGCCA TCTACCACAT TCCTGGTTTT GAGGTGCAGG ATACATCAGG GATCCTGGAA
    GAGCTGTGTG CACCTGCCTT CTTCCTCCAG GGCCTCCTCC AAACGTCGGA GGACACAGAC
    CTGCCCTACC CGCCACCCCA GAGAGAGGCC AACATCTACA TGGTTCCCCA GAACATCAAG
    CCTGCTCTCC AGCGGACGGC CATCGAGATC CTGGCATGGG GCCTGCGGAA CATGAAGAGT
    TACCACCTGG CCAGTGTCTC CTCCCCCAGC CTCGTGGTGG AGTGCGGGGG CCAGACGGTG
    CAGTCCTGTG TCATCAGGAA CCTCCAGAAG AACCCCAACT TCGACGTCTG CACCCTCTTC
    ATGGAAGTGA TGCTGCCCAG GGAGGAGCTC TACTGTCCCC CCATCGTGGT CAAGGTCATC
    GATAACTGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCTG CTCCCTGGAG
    AGCTTCCTGT GTGACCCCTA CTCAGAGGAG AGTCCGTCCC CACAGGGCGG CCCAGATGAT
    GTGAGCTTAC TCAGTCCTGG GGAAGATGTG CTCATTGACA TTGATGACAA GGAGCCCCTC
    ATCCCTATGC AGCTTGCAGA TGGTCTGTCG GGCTCGGCCC CCACTAACAT GGCATCTTCT
    CCGTCCAGTC CTCATGAGGA GGAGTTCATC GATTGGTGGA GCAAATTCTT TGCCTCGATA
    GGGGAGAGGG AAAAGTGCGG GTCCTACTTG GAGAAGGACT TTGACACTCT AAAGGTCTAT
    GACACACAAC TGGAGAATGT GGAGGCCTTT GAGGGCCTGT CTGACTTTTG TAACACCTTC
    AAGCTCTACC GGGGCAAGAC TCAGGAGGAG ATGGAAGACC CATCTGTCGT TGGGGAATTT
    AAGGGCCTCT TCAAAATTTA TCCCCTCCCA GAAGACCCGG CCATCCCCAT GCCCCCAAGA
    CAGTTCCACC AGCTAGCTGC CCAGGGACCC CAGGAGTGCC TGGTCCGCAT CTACATCATC
    CGGGCATTCG GCCTGCAGCC CAAGGACCCC AATGGGAAGT GTGACCCTTA CATCAAGATC
    TCCATAGGGA AGAAATCCGT GAGCGACCAG GATAACTACA TCCCCTGCAC TCTGGAGCCT
    GTGTTTGGAA AGATGTTCGA ACTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTGAAGATC
    ACTCTCTATG ACTATGACCT CCTCTCCAAG GACGAGATGA TCGGGGAGAC GGTCATCGAC
    CTGGAGAATA GGCTGCTGTC CAAGTTTGGA GCTCGCTGCG GACTCCCACA GACCTACTGT
    GTCTCTGGAC CGAACCAGTG GAGGGACCAG CTCCGCCCCT CCCAGCTTCT CCACCTGTTC
    TGCCAGCAGC AGAGAGTCAA GGCCCCCGTG TACCAGACCG ACCGTGTGGT GTTTGGGAAT
    AAAGAATACA CGATTGAAGA AATAGAGGCT GGGAGGGTCC TGAATCCACA CCTGGGCCCA
    GTGGAAGAGC GCCTGGCCTT GCACGTCCTC CAGCAGCAGG GCTTGGTGCC CGAGCATGTG
    GAGTCCCGGC CCCTCTATAG CCCCCTGCAG CCGGACATTG AGCAGGGGAA GCTGCAGATG
    TGGATCGACC TGTTTCCAAA GGCCCTGGGG CGGCCTGGAC CTCCCTTCAA CATCACTCCA
    CGGAGAGCCA GAAGGTTTTT CCTGCGTTGC ATTATCTGGA ACACCAAGGA TGTGATCCTG
    GACGACCTCA GCATCACAGG AGAGAAGATG AGCGACATTT ATGTGAAAGG TTGGATGGTT
    GGCTTTGAAG AACACAAGCA AAAGACAGAT GTGCATTATC GGTCCCTGGG AGGCGAAGGG
    AACTTTAACT GGAGGTTCAT TTTCCCCTTT GACTACCTGC TGGCTGAGCA AGTCTGCACC
    GTCTCCAAGA AGGATGCTTT CTGGAAGCTG GACAAGACCG AGAGCAAAAT CCCAGCACGA
    GTGGTATTCC AGATCTGGGA CAATGACAAG TTCTCCTTTG ATGATTTCCT GGGCTCCTTG
    CAGCTGGATC TCAACCGCAT GCCCAAGCCT GCCAAGACAG CCGAGAAGTG CTCCCTGGAC
    CAGCTGGATG ACACTTTCCA CCCAGAGTGG TTTGTGTCCC TTTTTGAACA GAAAACTGTG
    AAGGGCTGGT GGCCCTGTGT GGCGGAAGAG GGTGAGAAGA AGAAGCTGGC GGGCAAGCTG
    GAGATGACCT TGGAGATTGT GGCGGAGAGT GAGCATGAGG AGCGGCCCGC TGGCCAGGGC
    CGGGATGAGC CCAACATGAA CCCCAAGCTC GAGGACCCAA GGCGCCCTGA CACCTCCTTC
    CTCTGGTTCA CCTCCCCGTA CAAGACTATG AAGTTCATCC TGTGGCGGCG TTTCCGGTGT
    GCCATCATCC TCTTCATCAT CCTCTTCATC CTACTGCTGT TCTTGGGCAT CTTCGTTTAC
    GCCTTCCCGA ACTATGCTGC CATGAAGCTG GTGAAGCCCT TCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023474677.1
    CDS211..6618
    Translation

    Target ORF information:

    RefSeq Version XM_023618909.1
    Organism Equus caballus(horse)
    Definition Equus caballus dysferlin (DYSF), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023618909.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGTGCT GCCTGCTAGT GAAGGCCAGC AACCTTCCCA CTGTGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAA
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG GGTCCCCCTG
    GACCAGAGCT CTGAGCTTCA CGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
    TTCCTGGGAG AAGCCAAGGT CCCACTCCGG GAGGTCCTTG CCACCCCCAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACGCCTCT CCCTGGAGCT GTGCCCCTGT TCCCACCCTC TGTGCCGCTG
    GAACCCTCCC CGACTCTGCC CGATGTGGAT ATGCTGGCTG GTGGGGGACA GAGCCGGGCC
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAATGG
    CAGGCCCCCA CGGATACAGG AGGAGAGGAG GACACCGAGG ACCAGGGGCT CACGGGAGAT
    GAGGCAGAGC CATTCTTGGA TCAGAACGGA GCCCCAGGCC CCGGGGCTCC CACCACCCTG
    AAGAAGCCAC CTTCCCATCC TCCCCCCTAC CATCCTGGGG GGAAAAGGAA GAGAAGCACG
    CCTGCGCCCA GAAAGCTGCT TTCGGATAAA CCACAGGACT TCCAGATCAG GGTCCAGGTG
    ATCGAGGGGC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCGGCC
    AGGCAGACCA AGCGGACTCG GATTCACAGG GGAAACAGCC CGCTCTTCAA CGAGACTCTC
    TTCTTCAACG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGGCCGTCTT TATCACGGTG
    GTAGACTCCT GTTCGCTCCG GACAGATGCC CTCATCGGGG AGTTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCT
    GACGATTTCT CTGCTGGGCC CAAAGGCTAC CTGAAAGCAA GCCTTTGTGT GCTGGGGCCT
    GGAGACGAAG CTCCGCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAGC
    AATCTGCTCA GGCCAACTGG CATGGCCCTT CGAGGAGCGC ACTTCTGTCT GAAGGTCTTC
    AGGGCTGAGG ACTTACCACA GATGGACGAT GCCGTGGTGG ACAGCGTGAA GCAGATCTTC
    GGCTTTGACA GTAACAAGAA GAACCTGGTG GATCCCTTCG TCGAGGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ATGGCCAACC CTCAGTGGAA CCAGAGCATC
    ACGCTGCCTG TCATGTTTCC CTCCATGTCT GAAAAAATGA GGATTCGTGT CATAGACTGG
    GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAATATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGC AGTGGACGAC CACCTGGGCT TCCTCCCCAC CTTCGGGCCC
    TGCTATGTCA ACCTCTACGG CAGCCCCAGG GAGTTCACTG GCTTCCCAGA CCCCTATGCA
    GAGCTCAACA CGGGCAAGGG AGAAGGCGTG GCCTACCGAG GCCGGCTTCT GGTCTCCCTG
    GAGACCAAAC TGGTGGAGCA AGGCGAGCAG AAGGTGGAGG ATCTCGCTGC AGATGACATC
    CTTCGGGTGG AGAAGTACCT CCGGAGGCGC AAGTACTCCC TCTTTGCTGC CTTCTACTCG
    GCCTGCATGC TGCAGGATGT CGACGATGCC ATCCAGTTCG AGGTCAGCAT CGGAAACTAC
    GGGAACAAGT TTGACACGAC CTGCCTGCCG CTGGCCTCCA CCACCCAGTA CAGCCGGGCG
    GTCTTTGACG GGTGCCACTA CTACTACTTA CCCTGGGGCA ACGTGAAGCC TGTGGTGGTG
    CTGTCATCCT ACTGGGAAGA CATCAGCCAC AGAGTCGACA CTCAGAACCA GCTGCTCCGG
    ATCGCCGACC GGCTGGAAGC CGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCGGTGC
    TCCACCGAGG ATGTGGACTC CCTGGTGGCT CAGCTGATGG ATGAGCTCAT TGCAGACTGC
    AGCCAGCCCC TGTGCAACGT CCAAGAGACG CCCTCTGCCA CCCACCTGGA CCAGTACCTG
    TACCGGCTGC GCACCCGTCA CCTGAGCCAG ATCAGTGAGG CGGCCCTGGC CTTGAAGCTC
    GGCCACAGTG AGCTCCCCGC CGCCCTTGAG CAGGCTGAGG ACTGGCTCCT GCGTCTCCGT
    GCCCTGGCGG AGGAGCCCCA GAACAGCCTG CCTGACGTGG TCATCTGGAT GCTGCAGGGA
    GACAAGCGGG TGGCGTACCA GCGGGTGCCC GCCCACGAGG TCCTCTTCTC CCGGCGGGGC
    GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTGCAGACGA TCTTTCTGAA GTATCCAATG
    GAAGGGGTGC CGGGAGCCCG GATGCCGGTG CAGATACGGG TGAAGCTCTG GTTCGGGCTC
    TCTGTGGATG AGAAGGAGTT CAATCAGTTT GCTGAGGGCA AGCTCTCTGT CTTCGCTGAA
    ACCTACGAGA ACCAGACCAA GCTGGCCCTA GTCGGGAACT GGGGCACAAC GGGCCTCACC
    TACCCCAAGT TTTCTGACGT CACAGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC
    TCGGCCGGCT GGGCCTGGGC TGGAGAGTGG TTCGTGTGTC CGGAGAAGAC CCTACTCCAC
    GACACCGATG CGGGCCACCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTG
    CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACAG ATGTGAACGG GGAGAAGGTG
    CTTCCCAAGG ATGACATCGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA AGAATGGTCC
    ACAGACCTCA ATCGGGCCGT TGACGAGCAA GGCTGGGAGT ACAGCATCAC CATCCCTCCG
    GAACGGAAGC CAAGGCACTG GGTCCCTGCC GAGAAGATGT ACTACACACA CCGTCGGCGG
    CGCTGGGTCC GCCTGCGCAG GAGGGACCTC AGCCAGATGG AGGCGCTGAA GAGGCACAGG
    CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCGC TCTTCGGCTG GAAGTTCCAC
    CTGGAGTACC GCAAGACCGA TGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCA
    CTGGAGAAGA CAGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCCCTGGG CGGCGTGGTG
    GACGACAGGA GTGAAGATTC GGTGTCCGTC TCCACCTTGA GCTTCGGCGT GAACAGACCC
    ACGATTTCCT GCATCTTCGA CTACGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG
    GCCCGGGACC TGCCCGCCAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC AATCGTCTCC
    TTCCTGCACC AGAGCCAGAA GACAGTGGTG GTGAAGAACA CCCTGAACCC CACCTGGGAC
    CAGACACTCA TCTTCTATGA GATCGAGATC TTTGGTGAGC CGCCCAGCAT CGCCGAGCAG
    CCGCCCAGCA TCGTGGTGGA GCTATACGAC CATGACACCT ACGGTGCAGA TGAGTTTATG
    GGACGCTGCA TCTGTCAGCC GACTCTGGAA CGGATGCCCC GGCTGACCTG GTTCCCACTG
    ACTAGGGGCA GCCAGCCGGC GGGCGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG
    AAGCCGGCCA TCTACCACAT TCCTGGTTTT GAGGTGCAGG ATACATCAGG GATCCTGGAA
    GAGCTGTGTG CACCTGCCTT CTTCCTCCAG GGCCTCCTCC AAACGTCGGA GGACACAGAC
    CTGCCCTACC CGCCACCCCA GAGAGAGGCC AACATCTACA TGGTTCCCCA GAACATCAAG
    CCTGCTCTCC AGCGGACGGC CATCGAGATC CTGGCATGGG GCCTGCGGAA CATGAAGAGT
    TACCACCTGG CCAGTGTCTC CTCCCCCAGC CTCGTGGTGG AGTGCGGGGG CCAGACGGTG
    CAGTCCTGTG TCATCAGGAA CCTCCAGAAG AACCCCAACT TCGACGTCTG CACCCTCTTC
    ATGGAAGTGA TGCTGCCCAG GGAGGAGCTC TACTGTCCCC CCATCGTGGT CAAGGTCATC
    GATAACTGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCTG CTCCCTGGAG
    AGCTTCCTGT GTGACCCCTA CTCAGAGGAG AGTCCGTCCC CACAGGGCGG CCCAGATGAT
    GTGAGCTTAC TCAGTCCTGG GGAAGATGTG CTCATTGACA TTGATGACAA GGAGCCCCTC
    ATCCCTATGC AGCTTGCAGA TGGTCTGTCG GGCTCGGCCC CCACTAACAT GGCATCTTCT
    CCGTCCAGTC CTCATGAGGA GGAGTTCATC GATTGGTGGA GCAAATTCTT TGCCTCGATA
    GGGGAGAGGG AAAAGTGCGG GTCCTACTTG GAGAAGGACT TTGACACTCT AAAGGTCTAT
    GACACACAAC TGGAGAATGT GGAGGCCTTT GAGGGCCTGT CTGACTTTTG TAACACCTTC
    AAGCTCTACC GGGGCAAGAC TCAGGAGGAG ATGGAAGACC CATCTGTCGT TGGGGAATTT
    AAGGGCCTCT TCAAAATTTA TCCCCTCCCA GAAGACCCGG CCATCCCCAT GCCCCCAAGA
    CAGTTCCACC AGCTAGCTGC CCAGGGACCC CAGGAGTGCC TGGTCCGCAT CTACATCATC
    CGGGCATTCG GCCTGCAGCC CAAGGACCCC AATGGGAAGT GTGACCCTTA CATCAAGATC
    TCCATAGGGA AGAAATCCGT GAGCGACCAG GATAACTACA TCCCCTGCAC TCTGGAGCCT
    GTGTTTGGAA AGATGTTCGA ACTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTGAAGATC
    ACTCTCTATG ACTATGACCT CCTCTCCAAG GACGAGATGA TCGGGGAGAC GGTCATCGAC
    CTGGAGAATA GGCTGCTGTC CAAGTTTGGA GCTCGCTGCG GACTCCCACA GACCTACTGT
    GTCTCTGGAC CGAACCAGTG GAGGGACCAG CTCCGCCCCT CCCAGCTTCT CCACCTGTTC
    TGCCAGCAGC AGAGAGTCAA GGCCCCCGTG TACCAGACCG ACCGTGTGGT GTTTGGGAAT
    AAAGAATACA CGATTGAAGA AATAGAGGCT GGGAGGGTCC TGAATCCACA CCTGGGCCCA
    GTGGAAGAGC GCCTGGCCTT GCACGTCCTC CAGCAGCAGG GCTTGGTGCC CGAGCATGTG
    GAGTCCCGGC CCCTCTATAG CCCCCTGCAG CCGGACATTG AGCAGGGGAA GCTGCAGATG
    TGGATCGACC TGTTTCCAAA GGCCCTGGGG CGGCCTGGAC CTCCCTTCAA CATCACTCCA
    CGGAGAGCCA GAAGGTTTTT CCTGCGTTGC ATTATCTGGA ACACCAAGGA TGTGATCCTG
    GACGACCTCA GCATCACAGG AGAGAAGATG AGCGACATTT ATGTGAAAGG TTGGATGGTT
    GGCTTTGAAG AACACAAGCA AAAGACAGAT GTGCATTATC GGTCCCTGGG AGGCGAAGGG
    AACTTTAACT GGAGGTTCAT TTTCCCCTTT GACTACCTGC TGGCTGAGCA AGTCTGCACC
    GTCTCCAAGA AGGATGCTTT CTGGAAGCTG GACAAGACCG AGAGCAAAAT CCCAGCACGA
    GTGGTATTCC AGATCTGGGA CAATGACAAG TTCTCCTTTG ATGATTTCCT GGGCTCCTTG
    CAGCTGGATC TCAACCGCAT GCCCAAGCCT GCCAAGACAG CCGAGAAGTG CTCCCTGGAC
    CAGCTGGATG ACACTTTCCA CCCAGAGTGG TTTGTGTCCC TTTTTGAACA GAAAACTGTG
    AAGGGCTGGT GGCCCTGTGT GGCGGAAGAG GGTGAGAAGA AGAAGCTGGC GGGCAAGCTG
    GAGATGACCT TGGAGATTGT GGCGGAGAGT GAGCATGAGG AGCGGCCCGC TGGCCAGGGC
    CGGGATGAGC CCAACATGAA CCCCAAGCTC GAGGACCCAA GGCGCCCTGA CACCTCCTTC
    CTCTGGTTCA CCTCCCCGTA CAAGACTATG AAGTTCATCC TGTGGCGGCG TTTCCGGTGT
    GCCATCATCC TCTTCATCAT CCTCTTCATC CTACTGCTGT TCTTGGGCAT CTTCGTTTAC
    GCCTTCCCGA ACTATGCTGC CATGAAGCTG GTGAAGCCCT TCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb49778
    Clone ID Related Accession (Same CDS sequence) XM_023618910.1
    Accession Version XM_023618910.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6387bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product dysferlin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009158.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGTGCT GCCTGCTAGT GAAGGCCAGC AACCTTCCCA CTGTGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAA
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG GGTCCCCCTG
    GACCAGAGCT CTGAGCTTCA CGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
    TTCCTGGGAG AAGCCAAGGT CCCACTCCGG GAGGTCCTTG CCACCCCCAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACGCCTCT CCCTGGAGCT GTGCCCCTGT TCCCACCCTC TGTGCCGCTG
    GAACCCTCCC CGACTCTGCC CGATGTGGAT ATGCTGGCTG GTGGGGGACA GAGCCGGGCC
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAATGG
    CAGGCCCCCA CGGATACAGG AGGAGAGGAG GACACCGAGG ACCAGGGGCT CACGGGAGAT
    GAGGCAGAGC CATTCTTGGA TCAGAACGGA GCCCCAGGCC CCGGGGCTCC CACCACCCTG
    AAGAAGCCAC CTTCCCATCC TCCCCCCTAC CATCCTGGGG GGAAAAGGAA GAGAAGCACG
    CCTGCGCCCA GAAAGCTGCT TTCGGATAAA CCACAGGACT TCCAGATCAG GGTCCAGGTG
    ATCGAGGGGC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCGGCC
    AGGCAGACCA AGCGGACTCG GATTCACAGG GGAAACAGCC CGCTCTTCAA CGAGACTCTC
    TTCTTCAACG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGGCCGTCTT TATCACGGTG
    GTAGACTCCT GTTCGCTCCG GACAGATGCC CTCATCGGGG AGTTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCT
    GACGATTTCT CTGCTGGGCC CAAAGGCTAC CTGAAAGCAA GCCTTTGTGT GCTGGGGCCT
    GGAGACGAAG CTCCGCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAGC
    AATCTGCTCA GGCCAACTGG CATGGCCCTT CGAGGAGCGC ACTTCTGTCT GAAGGTCTTC
    AGGGCTGAGG ACTTACCACA GATGGACGAT GCCGTGGTGG ACAGCGTGAA GCAGATCTTC
    GGCTTTGACA GTAACAAGAA GAACCTGGTG GATCCCTTCG TCGAGGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ATGGCCAACC CTCAGTGGAA CCAGAGCATC
    ACGCTGCCTG TCATGTTTCC CTCCATGTCT GAAAAAATGA GGATTCGTGT CATAGACTGG
    GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAATATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGC AGAGGAGCCT GCAGGTGTGG TCAAGCCTCC TCCAGCCACG
    GACCTGGACG ACCACCTGGG CTTCCTCCCC ACCTTCGGGC CCTGCTATGT CAACCTCTAC
    GGCAGCCCCA GGGAGTTCAC TGGCTTCCCA GACCCCTATG CAGAGCTCAA CACGGGCAAG
    GGAGAAGGCG TGGCCTACCG AGGCCGGCTT CTGGTCTCCC TGGAGACCAA ACTGGTGGAG
    CAAGGCGAGC AGAAGGTGGA GGATCTCGCT GCAGATGACA TCCTTCGGGT GGAGAAGTAC
    CTCCGGAGGC GCAAGTACTC CCTCTTTGCT GCCTTCTACT CGGCCTGCAT GCTGCAGGAT
    GTCGACGATG CCATCCAGTT CGAGGTCAGC ATCGGAAACT ACGGGAACAA GTTTGACACG
    ACCTGCCTGC CGCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA CGGGTGCCAC
    TACTACTACT TACCCTGGGG CAACGTGAAG CCTGTGGTGG TGCTGTCATC CTACTGGGAA
    GACATCAGCC ACAGAGTCGA CACTCAGAAC CAGCTGCTCC GGATCGCCGA CCGGCTGGAA
    GCCGGCCTGG AGCAGGTCCA CCTGGCCCTG AAGGCGCGGT GCTCCACCGA GGATGTGGAC
    TCCCTGGTGG CTCAGCTGAT GGATGAGCTC ATTGCAGACT GCAGCCAGCC CCTGTGCAAC
    GTCCAAGAGA CGCCCTCTGC CACCCACCTG GACCAGTACC TGTACCGGCT GCGCACCCGT
    CACCTGAGCC AGATCAGTGA GGCGGCCCTG GCCTTGAAGC TCGGCCACAG TGAGCTCCCC
    GCCGCCCTTG AGCAGGCTGA GGACTGGCTC CTGCGTCTCC GTGCCCTGGC GGAGGAGCCC
    CAGAACAGCC TGCCTGACGT GGTCATCTGG ATGCTGCAGG GAGACAAGCG GGTGGCGTAC
    CAGCGGGTGC CCGCCCACGA GGTCCTCTTC TCCCGGCGGG GCGCCAACTA CTGTGGCAAG
    AATTGTGGGA AGCTGCAGAC GATCTTTCTG AAGTATCCAA TGGAAGGGGT GCCGGGAGCC
    CGGATGCCGG TGCAGATACG GGTGAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAG
    TTCAATCAGT TTGCTGAGGG CAAGCTCTCT GTCTTCGCTG AAACCTACGA GAACCAGACC
    AAGCTGGCCC TAGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAC
    GTCACAGGCA AGATCAAGCT ACCCAAGGAC AGCTTCCGCC CCTCGGCCGG CTGGGCCTGG
    GCTGGAGAGT GGTTCGTGTG TCCGGAGAAG ACCCTACTCC ACGACACCGA TGCGGGCCAC
    CTGAGCTTTG TGGAGGAGGT GTTTGAGAAC CAGACCCGGC TGCCCGGAGG CCAGTGGATC
    TACATGAGTG ACAACTACAC AGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC
    GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCC
    GTTGACGAGC AAGGCTGGGA GTACAGCATC ACCATCCCTC CGGAACGGAA GCCAAGGCAC
    TGGGTCCCTG CCGAGAAGAT GTACTACACA CACCGTCGGC GGCGCTGGGT CCGCCTGCGC
    AGGAGGGACC TCAGCCAGAT GGAGGCGCTG AAGAGGCACA GGCAGGCGGA GGCGGAGGGC
    GAGGGCTGGG AGTACGCCTC GCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACC
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CACTGGAGAA GACAGGGCCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCGTGG TGGACGACAG GAGTGAAGAT
    TCGGTGTCCG TCTCCACCTT GAGCTTCGGC GTGAACAGAC CCACGATTTC CTGCATCTTC
    GACTACGGGA ACCGCTACCA TCTACGCTGC TACATGTACC AGGCCCGGGA CCTGCCCGCC
    ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCAATCGTCT CCTTCCTGCA CCAGAGCCAG
    AAGACAGTGG TGGTGAAGAA CACCCTGAAC CCCACCTGGG ACCAGACACT CATCTTCTAT
    GAGATCGAGA TCTTTGGTGA GCCGCCCAGC ATCGCCGAGC AGCCGCCCAG CATCGTGGTG
    GAGCTATACG ACCATGACAC CTACGGTGCA GATGAGTTTA TGGGACGCTG CATCTGTCAG
    CCGACTCTGG AACGGATGCC CCGGCTGACC TGGTTCCCAC TGACTAGGGG CAGCCAGCCG
    GCGGGCGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCGGC CATCTACCAC
    ATTCCTGGTT TTGAGGTGCA GGATACATCA GGGATCCTGG AAGAGCTGTG TGCACCTGCC
    TTCTTCCTCC AGGGCCTCCT CCAAACGTCG GAGGACACAG ACCTGCCCTA CCCGCCACCC
    CAGAGAGAGG CCAACATCTA CATGGTTCCC CAGAACATCA AGCCTGCTCT CCAGCGGACG
    GCCATCGAGA TCCTGGCATG GGGCCTGCGG AACATGAAGA GTTACCACCT GGCCAGTGTC
    TCCTCCCCCA GCCTCGTGGT GGAGTGCGGG GGCCAGACGG TGCAGTCCTG TGTCATCAGG
    AACCTCCAGA AGAACCCCAA CTTCGACGTC TGCACCCTCT TCATGGAAGT GATGCTGCCC
    AGGGAGGAGC TCTACTGTCC CCCCATCGTG GTCAAGGTCA TCGATAACTG CCAGTTTGGC
    CGCCGGCCTG TGGTGGGCCA GTGTACCATC TGCTCCCTGG AGAGCTTCCT GTGTGACCCC
    TACTCAGAGG AGAGTCCGTC CCCACAGGGC GGCCCAGATG ATGTGAGCTT ACTCAGTCCT
    GGGGAAGATG TGCTCATTGA CATTGATGAC AAGGAGCCCC TCATCCCTAT GCAGGAGGAG
    GAGTTCATCG ATTGGTGGAG CAAATTCTTT GCCTCGATAG GGGAGAGGGA AAAGTGCGGG
    TCCTACTTGG AGAAGGACTT TGACACTCTA AAGGTCTATG ACACACAACT GGAGAATGTG
    GAGGCCTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTCTACCG GGGCAAGACT
    CAGGAGGAGA TGGAAGACCC ATCTGTCGTT GGGGAATTTA AGGGCCTCTT CAAAATTTAT
    CCCCTCCCAG AAGACCCGGC CATCCCCATG CCCCCAAGAC AGTTCCACCA GCTAGCTGCC
    CAGGGACCCC AGGAGTGCCT GGTCCGCATC TACATCATCC GGGCATTCGG CCTGCAGCCC
    AAGGACCCCA ATGGGAAGTG TGACCCTTAC ATCAAGATCT CCATAGGGAA GAAATCCGTG
    AGCGACCAGG ATAACTACAT CCCCTGCACT CTGGAGCCTG TGTTTGGAAA GATGTTCGAA
    CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGATCA CTCTCTATGA CTATGACCTC
    CTCTCCAAGG ACGAGATGAT CGGGGAGACG GTCATCGACC TGGAGAATAG GCTGCTGTCC
    AAGTTTGGAG CTCGCTGCGG ACTCCCACAG ACCTACTGTG TCTCTGGACC GAACCAGTGG
    AGGGACCAGC TCCGCCCCTC CCAGCTTCTC CACCTGTTCT GCCAGCAGCA GAGAGTCAAG
    GCCCCCGTGT ACCAGACCGA CCGTGTGGTG TTTGGGAATA AAGAATACAC GATTGAAGAA
    ATAGAGGCTG GGAGGGTCCT GAATCCACAC CTGGGCCCAG TGGAAGAGCG CCTGGCCTTG
    CACGTCCTCC AGCAGCAGGG CTTGGTGCCC GAGCATGTGG AGTCCCGGCC CCTCTATAGC
    CCCCTGCAGC CGGACATTGA GCAGGGGAAG CTGCAGATGT GGATCGACCT GTTTCCAAAG
    GCCCTGGGGC GGCCTGGACC TCCCTTCAAC ATCACTCCAC GGAGAGCCAG AAGGTTTTTC
    CTGCGTTGCA TTATCTGGAA CACCAAGGAT GTGATCCTGG ACGACCTCAG CATCACAGGA
    GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGGTTG GCTTTGAAGA ACACAAGCAA
    AAGACAGATG TGCATTATCG GTCCCTGGGA GGCGAAGGGA ACTTTAACTG GAGGTTCATT
    TTCCCCTTTG ACTACCTGCT GGCTGAGCAA GTCTGCACCG TCTCCAAGAA GGATGCTTTC
    TGGAAGCTGG ACAAGACCGA GAGCAAAATC CCAGCACGAG TGGTATTCCA GATCTGGGAC
    AATGACAAGT TCTCCTTTGA TGATTTCCTG GGCTCCTTGC AGCTGGATCT CAACCGCATG
    CCCAAGCCTG CCAAGACAGC CGAGAAGTGC TCCCTGGACC AGCTGGATGA CACTTTCCAC
    CCAGAGTGGT TTGTGTCCCT TTTTGAACAG AAAACTGTGA AGGGCTGGTG GCCCTGTGTG
    GCGGAAGAGG GTGAGAAGAA GAAGCTGGCG GGCAAGCTGG AGATGACCTT GGAGATTGTG
    GCGGAGAGTG AGCATGAGGA GCGGCCCGCT GGCCAGGGCC GGGATGAGCC CAACATGAAC
    CCCAAGCTCG AGGACCCAAG GCGCCCTGAC ACCTCCTTCC TCTGGTTCAC CTCCCCGTAC
    AAGACTATGA AGTTCATCCT GTGGCGGCGT TTCCGGTGTG CCATCATCCT CTTCATCATC
    CTCTTCATCC TACTGCTGTT CTTGGGCATC TTCGTTTACG CCTTCCCGAA CTATGCTGCC
    ATGAAGCTGG TGAAGCCCTT CAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023474678.1
    CDS211..6597
    Translation

    Target ORF information:

    RefSeq Version XM_023618910.1
    Organism Equus caballus(horse)
    Definition Equus caballus dysferlin (DYSF), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023618910.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGTGCT GCCTGCTAGT GAAGGCCAGC AACCTTCCCA CTGTGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAA
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG GGTCCCCCTG
    GACCAGAGCT CTGAGCTTCA CGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
    TTCCTGGGAG AAGCCAAGGT CCCACTCCGG GAGGTCCTTG CCACCCCCAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACGCCTCT CCCTGGAGCT GTGCCCCTGT TCCCACCCTC TGTGCCGCTG
    GAACCCTCCC CGACTCTGCC CGATGTGGAT ATGCTGGCTG GTGGGGGACA GAGCCGGGCC
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAATGG
    CAGGCCCCCA CGGATACAGG AGGAGAGGAG GACACCGAGG ACCAGGGGCT CACGGGAGAT
    GAGGCAGAGC CATTCTTGGA TCAGAACGGA GCCCCAGGCC CCGGGGCTCC CACCACCCTG
    AAGAAGCCAC CTTCCCATCC TCCCCCCTAC CATCCTGGGG GGAAAAGGAA GAGAAGCACG
    CCTGCGCCCA GAAAGCTGCT TTCGGATAAA CCACAGGACT TCCAGATCAG GGTCCAGGTG
    ATCGAGGGGC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCGGCC
    AGGCAGACCA AGCGGACTCG GATTCACAGG GGAAACAGCC CGCTCTTCAA CGAGACTCTC
    TTCTTCAACG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGGCCGTCTT TATCACGGTG
    GTAGACTCCT GTTCGCTCCG GACAGATGCC CTCATCGGGG AGTTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCT
    GACGATTTCT CTGCTGGGCC CAAAGGCTAC CTGAAAGCAA GCCTTTGTGT GCTGGGGCCT
    GGAGACGAAG CTCCGCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAGC
    AATCTGCTCA GGCCAACTGG CATGGCCCTT CGAGGAGCGC ACTTCTGTCT GAAGGTCTTC
    AGGGCTGAGG ACTTACCACA GATGGACGAT GCCGTGGTGG ACAGCGTGAA GCAGATCTTC
    GGCTTTGACA GTAACAAGAA GAACCTGGTG GATCCCTTCG TCGAGGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ATGGCCAACC CTCAGTGGAA CCAGAGCATC
    ACGCTGCCTG TCATGTTTCC CTCCATGTCT GAAAAAATGA GGATTCGTGT CATAGACTGG
    GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAATATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGC AGAGGAGCCT GCAGGTGTGG TCAAGCCTCC TCCAGCCACG
    GACCTGGACG ACCACCTGGG CTTCCTCCCC ACCTTCGGGC CCTGCTATGT CAACCTCTAC
    GGCAGCCCCA GGGAGTTCAC TGGCTTCCCA GACCCCTATG CAGAGCTCAA CACGGGCAAG
    GGAGAAGGCG TGGCCTACCG AGGCCGGCTT CTGGTCTCCC TGGAGACCAA ACTGGTGGAG
    CAAGGCGAGC AGAAGGTGGA GGATCTCGCT GCAGATGACA TCCTTCGGGT GGAGAAGTAC
    CTCCGGAGGC GCAAGTACTC CCTCTTTGCT GCCTTCTACT CGGCCTGCAT GCTGCAGGAT
    GTCGACGATG CCATCCAGTT CGAGGTCAGC ATCGGAAACT ACGGGAACAA GTTTGACACG
    ACCTGCCTGC CGCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA CGGGTGCCAC
    TACTACTACT TACCCTGGGG CAACGTGAAG CCTGTGGTGG TGCTGTCATC CTACTGGGAA
    GACATCAGCC ACAGAGTCGA CACTCAGAAC CAGCTGCTCC GGATCGCCGA CCGGCTGGAA
    GCCGGCCTGG AGCAGGTCCA CCTGGCCCTG AAGGCGCGGT GCTCCACCGA GGATGTGGAC
    TCCCTGGTGG CTCAGCTGAT GGATGAGCTC ATTGCAGACT GCAGCCAGCC CCTGTGCAAC
    GTCCAAGAGA CGCCCTCTGC CACCCACCTG GACCAGTACC TGTACCGGCT GCGCACCCGT
    CACCTGAGCC AGATCAGTGA GGCGGCCCTG GCCTTGAAGC TCGGCCACAG TGAGCTCCCC
    GCCGCCCTTG AGCAGGCTGA GGACTGGCTC CTGCGTCTCC GTGCCCTGGC GGAGGAGCCC
    CAGAACAGCC TGCCTGACGT GGTCATCTGG ATGCTGCAGG GAGACAAGCG GGTGGCGTAC
    CAGCGGGTGC CCGCCCACGA GGTCCTCTTC TCCCGGCGGG GCGCCAACTA CTGTGGCAAG
    AATTGTGGGA AGCTGCAGAC GATCTTTCTG AAGTATCCAA TGGAAGGGGT GCCGGGAGCC
    CGGATGCCGG TGCAGATACG GGTGAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAG
    TTCAATCAGT TTGCTGAGGG CAAGCTCTCT GTCTTCGCTG AAACCTACGA GAACCAGACC
    AAGCTGGCCC TAGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAC
    GTCACAGGCA AGATCAAGCT ACCCAAGGAC AGCTTCCGCC CCTCGGCCGG CTGGGCCTGG
    GCTGGAGAGT GGTTCGTGTG TCCGGAGAAG ACCCTACTCC ACGACACCGA TGCGGGCCAC
    CTGAGCTTTG TGGAGGAGGT GTTTGAGAAC CAGACCCGGC TGCCCGGAGG CCAGTGGATC
    TACATGAGTG ACAACTACAC AGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC
    GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCC
    GTTGACGAGC AAGGCTGGGA GTACAGCATC ACCATCCCTC CGGAACGGAA GCCAAGGCAC
    TGGGTCCCTG CCGAGAAGAT GTACTACACA CACCGTCGGC GGCGCTGGGT CCGCCTGCGC
    AGGAGGGACC TCAGCCAGAT GGAGGCGCTG AAGAGGCACA GGCAGGCGGA GGCGGAGGGC
    GAGGGCTGGG AGTACGCCTC GCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACC
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CACTGGAGAA GACAGGGCCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCGTGG TGGACGACAG GAGTGAAGAT
    TCGGTGTCCG TCTCCACCTT GAGCTTCGGC GTGAACAGAC CCACGATTTC CTGCATCTTC
    GACTACGGGA ACCGCTACCA TCTACGCTGC TACATGTACC AGGCCCGGGA CCTGCCCGCC
    ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCAATCGTCT CCTTCCTGCA CCAGAGCCAG
    AAGACAGTGG TGGTGAAGAA CACCCTGAAC CCCACCTGGG ACCAGACACT CATCTTCTAT
    GAGATCGAGA TCTTTGGTGA GCCGCCCAGC ATCGCCGAGC AGCCGCCCAG CATCGTGGTG
    GAGCTATACG ACCATGACAC CTACGGTGCA GATGAGTTTA TGGGACGCTG CATCTGTCAG
    CCGACTCTGG AACGGATGCC CCGGCTGACC TGGTTCCCAC TGACTAGGGG CAGCCAGCCG
    GCGGGCGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCGGC CATCTACCAC
    ATTCCTGGTT TTGAGGTGCA GGATACATCA GGGATCCTGG AAGAGCTGTG TGCACCTGCC
    TTCTTCCTCC AGGGCCTCCT CCAAACGTCG GAGGACACAG ACCTGCCCTA CCCGCCACCC
    CAGAGAGAGG CCAACATCTA CATGGTTCCC CAGAACATCA AGCCTGCTCT CCAGCGGACG
    GCCATCGAGA TCCTGGCATG GGGCCTGCGG AACATGAAGA GTTACCACCT GGCCAGTGTC
    TCCTCCCCCA GCCTCGTGGT GGAGTGCGGG GGCCAGACGG TGCAGTCCTG TGTCATCAGG
    AACCTCCAGA AGAACCCCAA CTTCGACGTC TGCACCCTCT TCATGGAAGT GATGCTGCCC
    AGGGAGGAGC TCTACTGTCC CCCCATCGTG GTCAAGGTCA TCGATAACTG CCAGTTTGGC
    CGCCGGCCTG TGGTGGGCCA GTGTACCATC TGCTCCCTGG AGAGCTTCCT GTGTGACCCC
    TACTCAGAGG AGAGTCCGTC CCCACAGGGC GGCCCAGATG ATGTGAGCTT ACTCAGTCCT
    GGGGAAGATG TGCTCATTGA CATTGATGAC AAGGAGCCCC TCATCCCTAT GCAGGAGGAG
    GAGTTCATCG ATTGGTGGAG CAAATTCTTT GCCTCGATAG GGGAGAGGGA AAAGTGCGGG
    TCCTACTTGG AGAAGGACTT TGACACTCTA AAGGTCTATG ACACACAACT GGAGAATGTG
    GAGGCCTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTCTACCG GGGCAAGACT
    CAGGAGGAGA TGGAAGACCC ATCTGTCGTT GGGGAATTTA AGGGCCTCTT CAAAATTTAT
    CCCCTCCCAG AAGACCCGGC CATCCCCATG CCCCCAAGAC AGTTCCACCA GCTAGCTGCC
    CAGGGACCCC AGGAGTGCCT GGTCCGCATC TACATCATCC GGGCATTCGG CCTGCAGCCC
    AAGGACCCCA ATGGGAAGTG TGACCCTTAC ATCAAGATCT CCATAGGGAA GAAATCCGTG
    AGCGACCAGG ATAACTACAT CCCCTGCACT CTGGAGCCTG TGTTTGGAAA GATGTTCGAA
    CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGATCA CTCTCTATGA CTATGACCTC
    CTCTCCAAGG ACGAGATGAT CGGGGAGACG GTCATCGACC TGGAGAATAG GCTGCTGTCC
    AAGTTTGGAG CTCGCTGCGG ACTCCCACAG ACCTACTGTG TCTCTGGACC GAACCAGTGG
    AGGGACCAGC TCCGCCCCTC CCAGCTTCTC CACCTGTTCT GCCAGCAGCA GAGAGTCAAG
    GCCCCCGTGT ACCAGACCGA CCGTGTGGTG TTTGGGAATA AAGAATACAC GATTGAAGAA
    ATAGAGGCTG GGAGGGTCCT GAATCCACAC CTGGGCCCAG TGGAAGAGCG CCTGGCCTTG
    CACGTCCTCC AGCAGCAGGG CTTGGTGCCC GAGCATGTGG AGTCCCGGCC CCTCTATAGC
    CCCCTGCAGC CGGACATTGA GCAGGGGAAG CTGCAGATGT GGATCGACCT GTTTCCAAAG
    GCCCTGGGGC GGCCTGGACC TCCCTTCAAC ATCACTCCAC GGAGAGCCAG AAGGTTTTTC
    CTGCGTTGCA TTATCTGGAA CACCAAGGAT GTGATCCTGG ACGACCTCAG CATCACAGGA
    GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGGTTG GCTTTGAAGA ACACAAGCAA
    AAGACAGATG TGCATTATCG GTCCCTGGGA GGCGAAGGGA ACTTTAACTG GAGGTTCATT
    TTCCCCTTTG ACTACCTGCT GGCTGAGCAA GTCTGCACCG TCTCCAAGAA GGATGCTTTC
    TGGAAGCTGG ACAAGACCGA GAGCAAAATC CCAGCACGAG TGGTATTCCA GATCTGGGAC
    AATGACAAGT TCTCCTTTGA TGATTTCCTG GGCTCCTTGC AGCTGGATCT CAACCGCATG
    CCCAAGCCTG CCAAGACAGC CGAGAAGTGC TCCCTGGACC AGCTGGATGA CACTTTCCAC
    CCAGAGTGGT TTGTGTCCCT TTTTGAACAG AAAACTGTGA AGGGCTGGTG GCCCTGTGTG
    GCGGAAGAGG GTGAGAAGAA GAAGCTGGCG GGCAAGCTGG AGATGACCTT GGAGATTGTG
    GCGGAGAGTG AGCATGAGGA GCGGCCCGCT GGCCAGGGCC GGGATGAGCC CAACATGAAC
    CCCAAGCTCG AGGACCCAAG GCGCCCTGAC ACCTCCTTCC TCTGGTTCAC CTCCCCGTAC
    AAGACTATGA AGTTCATCCT GTGGCGGCGT TTCCGGTGTG CCATCATCCT CTTCATCATC
    CTCTTCATCC TACTGCTGTT CTTGGGCATC TTCGTTTACG CCTTCCCGAA CTATGCTGCC
    ATGAAGCTGG TGAAGCCCTT CAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb49779
    Clone ID Related Accession (Same CDS sequence) XM_023618911.1
    Accession Version XM_023618911.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6378bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product dysferlin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009158.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGTGCT GCCTGCTAGT GAAGGCCAGC AACCTTCCCA CTGTGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAA
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG GGTCCCCCTG
    GACCAGAGCT CTGAGCTTCA CGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
    TTCCTGGGAG AAGCCAAGGT CCCACTCCGG GAGGTCCTTG CCACCCCCAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACGCCTCT CCCTGGAGCT GTGCCCCTGT TCCCACCCTC TGTGCCGCTG
    GAACCCTCCC CGACTCTGCC CGATGTGGAT ATGCTGGCTG GTGGGGGACA GAGCCGGGCC
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAATGG
    CAGGCCCCCA CGGATACAGG AGGAGAGGAG GACACCGAGG ACCAGGGGCT CACGGGAGAT
    GAGGCAGAGC CATTCTTGGA TCAGAACGGA GCCCCAGGCC CCGGGGCTCC CACCACCCTG
    AAGAAGCCAC CTTCCCATCC TCCCCCCTAC CATCCTGGGG GGAAAAGGAA GAGAAGCACG
    CCTGCGCCCA GAAAGCTGCT TTCGGATAAA CCACAGGACT TCCAGATCAG GGTCCAGGTG
    ATCGAGGGGC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCGGCC
    AGGCAGACCA AGCGGACTCG GATTCACAGG GGAAACAGCC CGCTCTTCAA CGAGACTCTC
    TTCTTCAACG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGGCCGTCTT TATCACGGTG
    GTAGACTCCT GTTCGCTCCG GACAGATGCC CTCATCGGGG AGTTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCT
    GACGATTTCT CTGCTGGGCC CAAAGGCTAC CTGAAAGCAA GCCTTTGTGT GCTGGGGCCT
    GGAGACGAAG CTCCGCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAGC
    AATCTGCTCA GGCCAACTGG CATGGCCCTT CGAGGAGCGC ACTTCTGTCT GAAGGTCTTC
    AGGGCTGAGG ACTTACCACA GATGGACGAT GCCGTGGTGG ACAGCGTGAA GCAGATCTTC
    GGCTTTGACA GTAACAAGAA GAACCTGGTG GATCCCTTCG TCGAGGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ATGGCCAACC CTCAGTGGAA CCAGAGCATC
    ACGCTGCCTG TCATGTTTCC CTCCATGTCT GAAAAAATGA GGATTCGTGT CATAGACTGG
    GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAATATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGC AGAGGAGCCT GCAGGTGTGG TCAAGCCTCC TCCAGCCACG
    GACCTGGACG ACCACCTGGG CTTCCTCCCC ACCTTCGGGC CCTGCTATGT CAACCTCTAC
    GGCAGCCCCA GGGAGTTCAC TGGCTTCCCA GACCCCTATG CAGAGCTCAA CACGGGCAAG
    GGAGAAGGCG TGGCCTACCG AGGCCGGCTT CTGGTCTCCC TGGAGACCAA ACTGGTGGAG
    CAAGGCGAGC AGAAGGTGGA GGATCTCGCT GCAGATGACA TCCTTCGGGT GGAGAAGTAC
    CTCCGGAGGC GCAAGTACTC CCTCTTTGCT GCCTTCTACT CGGCCTGCAT GCTGCAGGAT
    GTCGACGATG CCATCCAGTT CGAGGTCAGC ATCGGAAACT ACGGGAACAA GTTTGACACG
    ACCTGCCTGC CGCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA CGGGTGCCAC
    TACTACTACT TACCCTGGGG CAACGTGAAG CCTGTGGTGG TGCTGTCATC CTACTGGGAA
    GACATCAGCC ACAGAGTCGA CACTCAGAAC CAGCTGCTCC GGATCGCCGA CCGGCTGGAA
    GCCGGCCTGG AGCAGGTCCA CCTGGCCCTG AAGGCGCGGT GCTCCACCGA GGATGTGGAC
    TCCCTGGTGG CTCAGCTGAT GGATGAGCTC ATTGCAGACT GCAGCCAGCC CCTGTGCAAC
    GTCCAAGAGA CGCCCTCTGC CACCCACCTG GACCAGTACC TGTACCGGCT GCGCACCCGT
    CACCTGAGCC AGATCAGTGA GGCGGCCCTG GCCTTGAAGC TCGGCCACAG TGAGCTCCCC
    GCCGCCCTTG AGCAGGCTGA GGACTGGCTC CTGCGTCTCC GTGCCCTGGC GGAGGAGCCC
    CAGAACAGCC TGCCTGACGT GGTCATCTGG ATGCTGCAGG GAGACAAGCG GGTGGCGTAC
    CAGCGGGTGC CCGCCCACGA GGTCCTCTTC TCCCGGCGGG GCGCCAACTA CTGTGGCAAG
    AATTGTGGGA AGCTGCAGAC GATCTTTCTG AAGTATCCAA TGGAAGGGGT GCCGGGAGCC
    CGGATGCCGG TGCAGATACG GGTGAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAG
    TTCAATCAGT TTGCTGAGGG CAAGCTCTCT GTCTTCGCTG AAACCTACGA GAACCAGACC
    AAGCTGGCCC TAGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAC
    GTCACAGGCA AGATCAAGCT ACCCAAGGAC AGCTTCCGCC CCTCGGCCGG CTGGGCCTGG
    GCTGGAGAGT GGTTCGTGTG TCCGGAGAAG ACCCTACTCC ACGACACCGA TGCGGGCCAC
    CTGAGCTTTG TGGAGGAGGT GTTTGAGAAC CAGACCCGGC TGCCCGGAGG CCAGTGGATC
    TACATGAGTG ACAACTACAC AGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC
    GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCC
    GTTGACGAGC AAGGCTGGGA GTACAGCATC ACCATCCCTC CGGAACGGAA GCCAAGGCAC
    TGGGTCCCTG CCGAGAAGAT GTACTACACA CACCGTCGGC GGCGCTGGGT CCGCCTGCGC
    AGGAGGGACC TCAGCCAGAT GGAGGCGCTG AAGAGGCACA GGCAGGCGGA GGCGGAGGGC
    GAGGGCTGGG AGTACGCCTC GCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACC
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CACTGGAGAA GACAGGGCCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCGTGG TGGACGACAG GAGTGAAGAT
    TCGGTGTCCG TCTCCACCTT GAGCTTCGGC GTGAACAGAC CCACGATTTC CTGCATCTTC
    GACTACGGGA ACCGCTACCA TCTACGCTGC TACATGTACC AGGCCCGGGA CCTGCCCGCC
    ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCAATCGTCT CCTTCCTGCA CCAGAGCCAG
    AAGACAGTGG TGGTGAAGAA CACCCTGAAC CCCACCTGGG ACCAGACACT CATCTTCTAT
    GAGATCGAGA TCTTTGGTGA GCCGCCCAGC ATCGCCGAGC AGCCGCCCAG CATCGTGGTG
    GAGCTATACG ACCATGACAC CTACGGTGCA GATGAGTTTA TGGGACGCTG CATCTGTCAG
    CCGACTCTGG AACGGATGCC CCGGCTGACC TGGTTCCCAC TGACTAGGGG CAGCCAGCCG
    GCGGGCGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCGGC CATCTACCAC
    ATTCCTGGTT TTGAGTCGGA GGACACAGAC CTGCCCTACC CGCCACCCCA GAGAGAGGCC
    AACATCTACA TGGTTCCCCA GAACATCAAG CCTGCTCTCC AGCGGACGGC CATCGAGATC
    CTGGCATGGG GCCTGCGGAA CATGAAGAGT TACCACCTGG CCAGTGTCTC CTCCCCCAGC
    CTCGTGGTGG AGTGCGGGGG CCAGACGGTG CAGTCCTGTG TCATCAGGAA CCTCCAGAAG
    AACCCCAACT TCGACGTCTG CACCCTCTTC ATGGAAGTGA TGCTGCCCAG GGAGGAGCTC
    TACTGTCCCC CCATCGTGGT CAAGGTCATC GATAACTGCC AGTTTGGCCG CCGGCCTGTG
    GTGGGCCAGT GTACCATCTG CTCCCTGGAG AGCTTCCTGT GTGACCCCTA CTCAGAGGAG
    AGTCCGTCCC CACAGGGCGG CCCAGATGAT GTGAGCTTAC TCAGTCCTGG GGAAGATGTG
    CTCATTGACA TTGATGACAA GGAGCCCCTC ATCCCTATGC AGCTTGCAGA TGGTCTGTCG
    GGCTCGGCCC CCACTAACAT GGCATCTTCT CCGTCCAGTC CTCATGAGGA GGAGTTCATC
    GATTGGTGGA GCAAATTCTT TGCCTCGATA GGGGAGAGGG AAAAGTGCGG GTCCTACTTG
    GAGAAGGACT TTGACACTCT AAAGGTCTAT GACACACAAC TGGAGAATGT GGAGGCCTTT
    GAGGGCCTGT CTGACTTTTG TAACACCTTC AAGCTCTACC GGGGCAAGAC TCAGGAGGAG
    ATGGAAGACC CATCTGTCGT TGGGGAATTT AAGGGCCTCT TCAAAATTTA TCCCCTCCCA
    GAAGACCCGG CCATCCCCAT GCCCCCAAGA CAGTTCCACC AGCTAGCTGC CCAGGGACCC
    CAGGAGTGCC TGGTCCGCAT CTACATCATC CGGGCATTCG GCCTGCAGCC CAAGGACCCC
    AATGGGAAGT GTGACCCTTA CATCAAGATC TCCATAGGGA AGAAATCCGT GAGCGACCAG
    GATAACTACA TCCCCTGCAC TCTGGAGCCT GTGTTTGGAA AGATGTTCGA ACTGACCTGC
    ACTCTGCCTC TGGAGAAGGA CCTGAAGATC ACTCTCTATG ACTATGACCT CCTCTCCAAG
    GACGAGATGA TCGGGGAGAC GGTCATCGAC CTGGAGAATA GGCTGCTGTC CAAGTTTGGA
    GCTCGCTGCG GACTCCCACA GACCTACTGT GTCTCTGGAC CGAACCAGTG GAGGGACCAG
    CTCCGCCCCT CCCAGCTTCT CCACCTGTTC TGCCAGCAGC AGAGAGTCAA GGCCCCCGTG
    TACCAGACCG ACCGTGTGGT GTTTGGGAAT AAAGAATACA CGATTGAAGA AATAGAGGCT
    GGGAGGGTCC TGAATCCACA CCTGGGCCCA GTGGAAGAGC GCCTGGCCTT GCACGTCCTC
    CAGCAGCAGG GCTTGGTGCC CGAGCATGTG GAGTCCCGGC CCCTCTATAG CCCCCTGCAG
    CCGGACATTG AGCAGGGGAA GCTGCAGATG TGGATCGACC TGTTTCCAAA GGCCCTGGGG
    CGGCCTGGAC CTCCCTTCAA CATCACTCCA CGGAGAGCCA GAAGGTTTTT CCTGCGTTGC
    ATTATCTGGA ACACCAAGGA TGTGATCCTG GACGACCTCA GCATCACAGG AGAGAAGATG
    AGCGACATTT ATGTGAAAGG TTGGATGGTT GGCTTTGAAG AACACAAGCA AAAGACAGAT
    GTGCATTATC GGTCCCTGGG AGGCGAAGGG AACTTTAACT GGAGGTTCAT TTTCCCCTTT
    GACTACCTGC TGGCTGAGCA AGTCTGCACC GTCTCCAAGA AGGATGCTTT CTGGAAGCTG
    GACAAGACCG AGAGCAAAAT CCCAGCACGA GTGGTATTCC AGATCTGGGA CAATGACAAG
    TTCTCCTTTG ATGATTTCCT GGGCTCCTTG CAGCTGGATC TCAACCGCAT GCCCAAGCCT
    GCCAAGACAG CCGAGAAGTG CTCCCTGGAC CAGCTGGATG ACACTTTCCA CCCAGAGTGG
    TTTGTGTCCC TTTTTGAACA GAAAACTGTG AAGGGCTGGT GGCCCTGTGT GGCGGAAGAG
    GGTGAGAAGA AGAAGCTGGC GGGCAAGCTG GAGATGACCT TGGAGATTGT GGCGGAGAGT
    GAGCATGAGG AGCGGCCCGC TGGCCAGGGC CGGGATGAGC CCAACATGAA CCCCAAGCTC
    GAGGACCCAA GGCGCCCTGA CACCTCCTTC CTCTGGTTCA CCTCCCCGTA CAAGACTATG
    AAGTTCATCC TGTGGCGGCG TTTCCGGTGT GCCATCATCC TCTTCATCAT CCTCTTCATC
    CTACTGCTGT TCTTGGGCAT CTTCGTTTAC GCCTTCCCGA ACTATGCTGC CATGAAGCTG
    GTGAAGCCCT TCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023474679.1
    CDS211..6588
    Translation

    Target ORF information:

    RefSeq Version XM_023618911.1
    Organism Equus caballus(horse)
    Definition Equus caballus dysferlin (DYSF), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023618911.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGTGCT GCCTGCTAGT GAAGGCCAGC AACCTTCCCA CTGTGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAA
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG GGTCCCCCTG
    GACCAGAGCT CTGAGCTTCA CGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
    TTCCTGGGAG AAGCCAAGGT CCCACTCCGG GAGGTCCTTG CCACCCCCAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACGCCTCT CCCTGGAGCT GTGCCCCTGT TCCCACCCTC TGTGCCGCTG
    GAACCCTCCC CGACTCTGCC CGATGTGGAT ATGCTGGCTG GTGGGGGACA GAGCCGGGCC
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAATGG
    CAGGCCCCCA CGGATACAGG AGGAGAGGAG GACACCGAGG ACCAGGGGCT CACGGGAGAT
    GAGGCAGAGC CATTCTTGGA TCAGAACGGA GCCCCAGGCC CCGGGGCTCC CACCACCCTG
    AAGAAGCCAC CTTCCCATCC TCCCCCCTAC CATCCTGGGG GGAAAAGGAA GAGAAGCACG
    CCTGCGCCCA GAAAGCTGCT TTCGGATAAA CCACAGGACT TCCAGATCAG GGTCCAGGTG
    ATCGAGGGGC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCGGCC
    AGGCAGACCA AGCGGACTCG GATTCACAGG GGAAACAGCC CGCTCTTCAA CGAGACTCTC
    TTCTTCAACG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGGCCGTCTT TATCACGGTG
    GTAGACTCCT GTTCGCTCCG GACAGATGCC CTCATCGGGG AGTTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCT
    GACGATTTCT CTGCTGGGCC CAAAGGCTAC CTGAAAGCAA GCCTTTGTGT GCTGGGGCCT
    GGAGACGAAG CTCCGCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAGC
    AATCTGCTCA GGCCAACTGG CATGGCCCTT CGAGGAGCGC ACTTCTGTCT GAAGGTCTTC
    AGGGCTGAGG ACTTACCACA GATGGACGAT GCCGTGGTGG ACAGCGTGAA GCAGATCTTC
    GGCTTTGACA GTAACAAGAA GAACCTGGTG GATCCCTTCG TCGAGGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ATGGCCAACC CTCAGTGGAA CCAGAGCATC
    ACGCTGCCTG TCATGTTTCC CTCCATGTCT GAAAAAATGA GGATTCGTGT CATAGACTGG
    GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAATATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGC AGAGGAGCCT GCAGGTGTGG TCAAGCCTCC TCCAGCCACG
    GACCTGGACG ACCACCTGGG CTTCCTCCCC ACCTTCGGGC CCTGCTATGT CAACCTCTAC
    GGCAGCCCCA GGGAGTTCAC TGGCTTCCCA GACCCCTATG CAGAGCTCAA CACGGGCAAG
    GGAGAAGGCG TGGCCTACCG AGGCCGGCTT CTGGTCTCCC TGGAGACCAA ACTGGTGGAG
    CAAGGCGAGC AGAAGGTGGA GGATCTCGCT GCAGATGACA TCCTTCGGGT GGAGAAGTAC
    CTCCGGAGGC GCAAGTACTC CCTCTTTGCT GCCTTCTACT CGGCCTGCAT GCTGCAGGAT
    GTCGACGATG CCATCCAGTT CGAGGTCAGC ATCGGAAACT ACGGGAACAA GTTTGACACG
    ACCTGCCTGC CGCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA CGGGTGCCAC
    TACTACTACT TACCCTGGGG CAACGTGAAG CCTGTGGTGG TGCTGTCATC CTACTGGGAA
    GACATCAGCC ACAGAGTCGA CACTCAGAAC CAGCTGCTCC GGATCGCCGA CCGGCTGGAA
    GCCGGCCTGG AGCAGGTCCA CCTGGCCCTG AAGGCGCGGT GCTCCACCGA GGATGTGGAC
    TCCCTGGTGG CTCAGCTGAT GGATGAGCTC ATTGCAGACT GCAGCCAGCC CCTGTGCAAC
    GTCCAAGAGA CGCCCTCTGC CACCCACCTG GACCAGTACC TGTACCGGCT GCGCACCCGT
    CACCTGAGCC AGATCAGTGA GGCGGCCCTG GCCTTGAAGC TCGGCCACAG TGAGCTCCCC
    GCCGCCCTTG AGCAGGCTGA GGACTGGCTC CTGCGTCTCC GTGCCCTGGC GGAGGAGCCC
    CAGAACAGCC TGCCTGACGT GGTCATCTGG ATGCTGCAGG GAGACAAGCG GGTGGCGTAC
    CAGCGGGTGC CCGCCCACGA GGTCCTCTTC TCCCGGCGGG GCGCCAACTA CTGTGGCAAG
    AATTGTGGGA AGCTGCAGAC GATCTTTCTG AAGTATCCAA TGGAAGGGGT GCCGGGAGCC
    CGGATGCCGG TGCAGATACG GGTGAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAG
    TTCAATCAGT TTGCTGAGGG CAAGCTCTCT GTCTTCGCTG AAACCTACGA GAACCAGACC
    AAGCTGGCCC TAGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAC
    GTCACAGGCA AGATCAAGCT ACCCAAGGAC AGCTTCCGCC CCTCGGCCGG CTGGGCCTGG
    GCTGGAGAGT GGTTCGTGTG TCCGGAGAAG ACCCTACTCC ACGACACCGA TGCGGGCCAC
    CTGAGCTTTG TGGAGGAGGT GTTTGAGAAC CAGACCCGGC TGCCCGGAGG CCAGTGGATC
    TACATGAGTG ACAACTACAC AGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC
    GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCC
    GTTGACGAGC AAGGCTGGGA GTACAGCATC ACCATCCCTC CGGAACGGAA GCCAAGGCAC
    TGGGTCCCTG CCGAGAAGAT GTACTACACA CACCGTCGGC GGCGCTGGGT CCGCCTGCGC
    AGGAGGGACC TCAGCCAGAT GGAGGCGCTG AAGAGGCACA GGCAGGCGGA GGCGGAGGGC
    GAGGGCTGGG AGTACGCCTC GCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACC
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CACTGGAGAA GACAGGGCCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCGTGG TGGACGACAG GAGTGAAGAT
    TCGGTGTCCG TCTCCACCTT GAGCTTCGGC GTGAACAGAC CCACGATTTC CTGCATCTTC
    GACTACGGGA ACCGCTACCA TCTACGCTGC TACATGTACC AGGCCCGGGA CCTGCCCGCC
    ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCAATCGTCT CCTTCCTGCA CCAGAGCCAG
    AAGACAGTGG TGGTGAAGAA CACCCTGAAC CCCACCTGGG ACCAGACACT CATCTTCTAT
    GAGATCGAGA TCTTTGGTGA GCCGCCCAGC ATCGCCGAGC AGCCGCCCAG CATCGTGGTG
    GAGCTATACG ACCATGACAC CTACGGTGCA GATGAGTTTA TGGGACGCTG CATCTGTCAG
    CCGACTCTGG AACGGATGCC CCGGCTGACC TGGTTCCCAC TGACTAGGGG CAGCCAGCCG
    GCGGGCGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCGGC CATCTACCAC
    ATTCCTGGTT TTGAGTCGGA GGACACAGAC CTGCCCTACC CGCCACCCCA GAGAGAGGCC
    AACATCTACA TGGTTCCCCA GAACATCAAG CCTGCTCTCC AGCGGACGGC CATCGAGATC
    CTGGCATGGG GCCTGCGGAA CATGAAGAGT TACCACCTGG CCAGTGTCTC CTCCCCCAGC
    CTCGTGGTGG AGTGCGGGGG CCAGACGGTG CAGTCCTGTG TCATCAGGAA CCTCCAGAAG
    AACCCCAACT TCGACGTCTG CACCCTCTTC ATGGAAGTGA TGCTGCCCAG GGAGGAGCTC
    TACTGTCCCC CCATCGTGGT CAAGGTCATC GATAACTGCC AGTTTGGCCG CCGGCCTGTG
    GTGGGCCAGT GTACCATCTG CTCCCTGGAG AGCTTCCTGT GTGACCCCTA CTCAGAGGAG
    AGTCCGTCCC CACAGGGCGG CCCAGATGAT GTGAGCTTAC TCAGTCCTGG GGAAGATGTG
    CTCATTGACA TTGATGACAA GGAGCCCCTC ATCCCTATGC AGCTTGCAGA TGGTCTGTCG
    GGCTCGGCCC CCACTAACAT GGCATCTTCT CCGTCCAGTC CTCATGAGGA GGAGTTCATC
    GATTGGTGGA GCAAATTCTT TGCCTCGATA GGGGAGAGGG AAAAGTGCGG GTCCTACTTG
    GAGAAGGACT TTGACACTCT AAAGGTCTAT GACACACAAC TGGAGAATGT GGAGGCCTTT
    GAGGGCCTGT CTGACTTTTG TAACACCTTC AAGCTCTACC GGGGCAAGAC TCAGGAGGAG
    ATGGAAGACC CATCTGTCGT TGGGGAATTT AAGGGCCTCT TCAAAATTTA TCCCCTCCCA
    GAAGACCCGG CCATCCCCAT GCCCCCAAGA CAGTTCCACC AGCTAGCTGC CCAGGGACCC
    CAGGAGTGCC TGGTCCGCAT CTACATCATC CGGGCATTCG GCCTGCAGCC CAAGGACCCC
    AATGGGAAGT GTGACCCTTA CATCAAGATC TCCATAGGGA AGAAATCCGT GAGCGACCAG
    GATAACTACA TCCCCTGCAC TCTGGAGCCT GTGTTTGGAA AGATGTTCGA ACTGACCTGC
    ACTCTGCCTC TGGAGAAGGA CCTGAAGATC ACTCTCTATG ACTATGACCT CCTCTCCAAG
    GACGAGATGA TCGGGGAGAC GGTCATCGAC CTGGAGAATA GGCTGCTGTC CAAGTTTGGA
    GCTCGCTGCG GACTCCCACA GACCTACTGT GTCTCTGGAC CGAACCAGTG GAGGGACCAG
    CTCCGCCCCT CCCAGCTTCT CCACCTGTTC TGCCAGCAGC AGAGAGTCAA GGCCCCCGTG
    TACCAGACCG ACCGTGTGGT GTTTGGGAAT AAAGAATACA CGATTGAAGA AATAGAGGCT
    GGGAGGGTCC TGAATCCACA CCTGGGCCCA GTGGAAGAGC GCCTGGCCTT GCACGTCCTC
    CAGCAGCAGG GCTTGGTGCC CGAGCATGTG GAGTCCCGGC CCCTCTATAG CCCCCTGCAG
    CCGGACATTG AGCAGGGGAA GCTGCAGATG TGGATCGACC TGTTTCCAAA GGCCCTGGGG
    CGGCCTGGAC CTCCCTTCAA CATCACTCCA CGGAGAGCCA GAAGGTTTTT CCTGCGTTGC
    ATTATCTGGA ACACCAAGGA TGTGATCCTG GACGACCTCA GCATCACAGG AGAGAAGATG
    AGCGACATTT ATGTGAAAGG TTGGATGGTT GGCTTTGAAG AACACAAGCA AAAGACAGAT
    GTGCATTATC GGTCCCTGGG AGGCGAAGGG AACTTTAACT GGAGGTTCAT TTTCCCCTTT
    GACTACCTGC TGGCTGAGCA AGTCTGCACC GTCTCCAAGA AGGATGCTTT CTGGAAGCTG
    GACAAGACCG AGAGCAAAAT CCCAGCACGA GTGGTATTCC AGATCTGGGA CAATGACAAG
    TTCTCCTTTG ATGATTTCCT GGGCTCCTTG CAGCTGGATC TCAACCGCAT GCCCAAGCCT
    GCCAAGACAG CCGAGAAGTG CTCCCTGGAC CAGCTGGATG ACACTTTCCA CCCAGAGTGG
    TTTGTGTCCC TTTTTGAACA GAAAACTGTG AAGGGCTGGT GGCCCTGTGT GGCGGAAGAG
    GGTGAGAAGA AGAAGCTGGC GGGCAAGCTG GAGATGACCT TGGAGATTGT GGCGGAGAGT
    GAGCATGAGG AGCGGCCCGC TGGCCAGGGC CGGGATGAGC CCAACATGAA CCCCAAGCTC
    GAGGACCCAA GGCGCCCTGA CACCTCCTTC CTCTGGTTCA CCTCCCCGTA CAAGACTATG
    AAGTTCATCC TGTGGCGGCG TTTCCGGTGT GCCATCATCC TCTTCATCAT CCTCTTCATC
    CTACTGCTGT TCTTGGGCAT CTTCGTTTAC GCCTTCCCGA ACTATGCTGC CATGAAGCTG
    GTGAAGCCCT TCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb49780
    Clone ID Related Accession (Same CDS sequence) XM_023618912.1
    Accession Version XM_023618912.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6366bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product dysferlin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009158.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGTGCT GCCTGCTAGT GAAGGCCAGC AACCTTCCCA CTGTGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAA
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG GGTCCCCCTG
    GACCAGAGCT CTGAGCTTCA CGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
    TTCCTGGGAG AAGCCAAGGT CCCACTCCGG GAGGTCCTTG CCACCCCCAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACGCCTCT CCCTGGAGCT GTGCCCCTGT TCCCACCCTC TGTGCCGCTG
    GAACCCTCCC CGACTCTGCC CGATGTGGAT ATGCTGGCTG GTGGGGGACA GAGCCGGGCC
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAATGG
    CAGGCCCCCA CGGATACAGG AGGAGAGGAG GACACCGAGG ACCAGGGGCT CACGGGAGAT
    GAGGCAGAGC CATTCTTGGA TCAGAACGGA GCCCCAGGCC CCGGGGCTCC CACCACCCTG
    AAGAAGCCAC CTTCCCATCC TCCCCCCTAC CATCCTGGGG GGAAAAGGAA GAGAAGCACG
    CCTGCGCCCA GAAAGCTGCT TTCGGATAAA CCACAGGACT TCCAGATCAG GGTCCAGGTG
    ATCGAGGGGC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCGGCC
    AGGCAGACCA AGCGGACTCG GATTCACAGG GGAAACAGCC CGCTCTTCAA CGAGACTCTC
    TTCTTCAACG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGGCCGTCTT TATCACGGTG
    GTAGACTCCT GTTCGCTCCG GACAGATGCC CTCATCGGGG AGTTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCT
    GACGATTTCT CTGCTGGGCC CAAAGGCTAC CTGAAAGCAA GCCTTTGTGT GCTGGGGCCT
    GGAGACGAAG CTCCGCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAGC
    AATCTGCTCA GGCCAACTGG CATGGCCCTT CGAGGAGCGC ACTTCTGTCT GAAGGTCTTC
    AGGGCTGAGG ACTTACCACA GATGGACGAT GCCGTGGTGG ACAGCGTGAA GCAGATCTTC
    GGCTTTGACA GTAACAAGAA GAACCTGGTG GATCCCTTCG TCGAGGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ATGGCCAACC CTCAGTGGAA CCAGAGCATC
    ACGCTGCCTG TCATGTTTCC CTCCATGTCT GAAAAAATGA GGATTCGTGT CATAGACTGG
    GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAATATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGC AGTGGACGAC CACCTGGGCT TCCTCCCCAC CTTCGGGCCC
    TGCTATGTCA ACCTCTACGG CAGCCCCAGG GAGTTCACTG GCTTCCCAGA CCCCTATGCA
    GAGCTCAACA CGGGCAAGGG AGAAGGCGTG GCCTACCGAG GCCGGCTTCT GGTCTCCCTG
    GAGACCAAAC TGGTGGAGCA AGGCGAGCAG AAGGTGGAGG ATCTCGCTGC AGATGACATC
    CTTCGGGTGG AGAAGTACCT CCGGAGGCGC AAGTACTCCC TCTTTGCTGC CTTCTACTCG
    GCCTGCATGC TGCAGGATGT CGACGATGCC ATCCAGTTCG AGGTCAGCAT CGGAAACTAC
    GGGAACAAGT TTGACACGAC CTGCCTGCCG CTGGCCTCCA CCACCCAGTA CAGCCGGGCG
    GTCTTTGACG GGTGCCACTA CTACTACTTA CCCTGGGGCA ACGTGAAGCC TGTGGTGGTG
    CTGTCATCCT ACTGGGAAGA CATCAGCCAC AGAGTCGACA CTCAGAACCA GCTGCTCCGG
    ATCGCCGACC GGCTGGAAGC CGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCGGTGC
    TCCACCGAGG ATGTGGACTC CCTGGTGGCT CAGCTGATGG ATGAGCTCAT TGCAGACTGC
    AGCCAGCCCC TGTGCAACGT CCAAGAGACG CCCTCTGCCA CCCACCTGGA CCAGTACCTG
    TACCGGCTGC GCACCCGTCA CCTGAGCCAG ATCAGTGAGG CGGCCCTGGC CTTGAAGCTC
    GGCCACAGTG AGCTCCCCGC CGCCCTTGAG CAGGCTGAGG ACTGGCTCCT GCGTCTCCGT
    GCCCTGGCGG AGGAGCCCCA GAACAGCCTG CCTGACGTGG TCATCTGGAT GCTGCAGGGA
    GACAAGCGGG TGGCGTACCA GCGGGTGCCC GCCCACGAGG TCCTCTTCTC CCGGCGGGGC
    GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTGCAGACGA TCTTTCTGAA GTATCCAATG
    GAAGGGGTGC CGGGAGCCCG GATGCCGGTG CAGATACGGG TGAAGCTCTG GTTCGGGCTC
    TCTGTGGATG AGAAGGAGTT CAATCAGTTT GCTGAGGGCA AGCTCTCTGT CTTCGCTGAA
    ACCTACGAGA ACCAGACCAA GCTGGCCCTA GTCGGGAACT GGGGCACAAC GGGCCTCACC
    TACCCCAAGT TTTCTGACGT CACAGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC
    TCGGCCGGCT GGGCCTGGGC TGGAGAGTGG TTCGTGTGTC CGGAGAAGAC CCTACTCCAC
    GACACCGATG CGGGCCACCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTG
    CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACAG ATGTGAACGG GGAGAAGGTG
    CTTCCCAAGG ATGACATCGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA AGAATGGTCC
    ACAGACCTCA ATCGGGCCGT TGACGAGCAA GGCTGGGAGT ACAGCATCAC CATCCCTCCG
    GAACGGAAGC CAAGGCACTG GGTCCCTGCC GAGAAGATGT ACTACACACA CCGTCGGCGG
    CGCTGGGTCC GCCTGCGCAG GAGGGACCTC AGCCAGATGG AGGCGCTGAA GAGGCACAGG
    CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCGC TCTTCGGCTG GAAGTTCCAC
    CTGGAGTACC GCAAGACCGA TGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCA
    CTGGAGAAGA CAGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCCCTGGG CGGCGTGGTG
    GACGACAGGA GTGAAGATTC GGTGTCCGTC TCCACCTTGA GCTTCGGCGT GAACAGACCC
    ACGATTTCCT GCATCTTCGA CTACGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG
    GCCCGGGACC TGCCCGCCAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC AATCGTCTCC
    TTCCTGCACC AGAGCCAGAA GACAGTGGTG GTGAAGAACA CCCTGAACCC CACCTGGGAC
    CAGACACTCA TCTTCTATGA GATCGAGATC TTTGGTGAGC CGCCCAGCAT CGCCGAGCAG
    CCGCCCAGCA TCGTGGTGGA GCTATACGAC CATGACACCT ACGGTGCAGA TGAGTTTATG
    GGACGCTGCA TCTGTCAGCC GACTCTGGAA CGGATGCCCC GGCTGACCTG GTTCCCACTG
    ACTAGGGGCA GCCAGCCGGC GGGCGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG
    AAGCCGGCCA TCTACCACAT TCCTGGTTTT GAGGTGCAGG ATACATCAGG GATCCTGGAA
    GAGTCGGAGG ACACAGACCT GCCCTACCCG CCACCCCAGA GAGAGGCCAA CATCTACATG
    GTTCCCCAGA ACATCAAGCC TGCTCTCCAG CGGACGGCCA TCGAGATCCT GGCATGGGGC
    CTGCGGAACA TGAAGAGTTA CCACCTGGCC AGTGTCTCCT CCCCCAGCCT CGTGGTGGAG
    TGCGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCAGAAGAA CCCCAACTTC
    GACGTCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGTCCCCCC
    ATCGTGGTCA AGGTCATCGA TAACTGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT
    ACCATCTGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CAGAGGAGAG TCCGTCCCCA
    CAGGGCGGCC CAGATGATGT GAGCTTACTC AGTCCTGGGG AAGATGTGCT CATTGACATT
    GATGACAAGG AGCCCCTCAT CCCTATGCAG CTTGCAGATG GTCTGTCGGG CTCGGCCCCC
    ACTAACATGG CATCTTCTCC GTCCAGTCCT CATGAGGAGG AGTTCATCGA TTGGTGGAGC
    AAATTCTTTG CCTCGATAGG GGAGAGGGAA AAGTGCGGGT CCTACTTGGA GAAGGACTTT
    GACACTCTAA AGGTCTATGA CACACAACTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT
    GACTTTTGTA ACACCTTCAA GCTCTACCGG GGCAAGACTC AGGAGGAGAT GGAAGACCCA
    TCTGTCGTTG GGGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCGGCC
    ATCCCCATGC CCCCAAGACA GTTCCACCAG CTAGCTGCCC AGGGACCCCA GGAGTGCCTG
    GTCCGCATCT ACATCATCCG GGCATTCGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT
    GACCCTTACA TCAAGATCTC CATAGGGAAG AAATCCGTGA GCGACCAGGA TAACTACATC
    CCCTGCACTC TGGAGCCTGT GTTTGGAAAG ATGTTCGAAC TGACCTGCAC TCTGCCTCTG
    GAGAAGGACC TGAAGATCAC TCTCTATGAC TATGACCTCC TCTCCAAGGA CGAGATGATC
    GGGGAGACGG TCATCGACCT GGAGAATAGG CTGCTGTCCA AGTTTGGAGC TCGCTGCGGA
    CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGA GGGACCAGCT CCGCCCCTCC
    CAGCTTCTCC ACCTGTTCTG CCAGCAGCAG AGAGTCAAGG CCCCCGTGTA CCAGACCGAC
    CGTGTGGTGT TTGGGAATAA AGAATACACG ATTGAAGAAA TAGAGGCTGG GAGGGTCCTG
    AATCCACACC TGGGCCCAGT GGAAGAGCGC CTGGCCTTGC ACGTCCTCCA GCAGCAGGGC
    TTGGTGCCCG AGCATGTGGA GTCCCGGCCC CTCTATAGCC CCCTGCAGCC GGACATTGAG
    CAGGGGAAGC TGCAGATGTG GATCGACCTG TTTCCAAAGG CCCTGGGGCG GCCTGGACCT
    CCCTTCAACA TCACTCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGCAT TATCTGGAAC
    ACCAAGGATG TGATCCTGGA CGACCTCAGC ATCACAGGAG AGAAGATGAG CGACATTTAT
    GTGAAAGGTT GGATGGTTGG CTTTGAAGAA CACAAGCAAA AGACAGATGT GCATTATCGG
    TCCCTGGGAG GCGAAGGGAA CTTTAACTGG AGGTTCATTT TCCCCTTTGA CTACCTGCTG
    GCTGAGCAAG TCTGCACCGT CTCCAAGAAG GATGCTTTCT GGAAGCTGGA CAAGACCGAG
    AGCAAAATCC CAGCACGAGT GGTATTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT
    GATTTCCTGG GCTCCTTGCA GCTGGATCTC AACCGCATGC CCAAGCCTGC CAAGACAGCC
    GAGAAGTGCT CCCTGGACCA GCTGGATGAC ACTTTCCACC CAGAGTGGTT TGTGTCCCTT
    TTTGAACAGA AAACTGTGAA GGGCTGGTGG CCCTGTGTGG CGGAAGAGGG TGAGAAGAAG
    AAGCTGGCGG GCAAGCTGGA GATGACCTTG GAGATTGTGG CGGAGAGTGA GCATGAGGAG
    CGGCCCGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CCAAGCTCGA GGACCCAAGG
    CGCCCTGACA CCTCCTTCCT CTGGTTCACC TCCCCGTACA AGACTATGAA GTTCATCCTG
    TGGCGGCGTT TCCGGTGTGC CATCATCCTC TTCATCATCC TCTTCATCCT ACTGCTGTTC
    TTGGGCATCT TCGTTTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023474680.1
    CDS211..6576
    Translation

    Target ORF information:

    RefSeq Version XM_023618912.1
    Organism Equus caballus(horse)
    Definition Equus caballus dysferlin (DYSF), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023618912.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGTGCT GCCTGCTAGT GAAGGCCAGC AACCTTCCCA CTGTGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAA
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG GGTCCCCCTG
    GACCAGAGCT CTGAGCTTCA CGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
    TTCCTGGGAG AAGCCAAGGT CCCACTCCGG GAGGTCCTTG CCACCCCCAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACGCCTCT CCCTGGAGCT GTGCCCCTGT TCCCACCCTC TGTGCCGCTG
    GAACCCTCCC CGACTCTGCC CGATGTGGAT ATGCTGGCTG GTGGGGGACA GAGCCGGGCC
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAATGG
    CAGGCCCCCA CGGATACAGG AGGAGAGGAG GACACCGAGG ACCAGGGGCT CACGGGAGAT
    GAGGCAGAGC CATTCTTGGA TCAGAACGGA GCCCCAGGCC CCGGGGCTCC CACCACCCTG
    AAGAAGCCAC CTTCCCATCC TCCCCCCTAC CATCCTGGGG GGAAAAGGAA GAGAAGCACG
    CCTGCGCCCA GAAAGCTGCT TTCGGATAAA CCACAGGACT TCCAGATCAG GGTCCAGGTG
    ATCGAGGGGC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCGGCC
    AGGCAGACCA AGCGGACTCG GATTCACAGG GGAAACAGCC CGCTCTTCAA CGAGACTCTC
    TTCTTCAACG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGGCCGTCTT TATCACGGTG
    GTAGACTCCT GTTCGCTCCG GACAGATGCC CTCATCGGGG AGTTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCT
    GACGATTTCT CTGCTGGGCC CAAAGGCTAC CTGAAAGCAA GCCTTTGTGT GCTGGGGCCT
    GGAGACGAAG CTCCGCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAGC
    AATCTGCTCA GGCCAACTGG CATGGCCCTT CGAGGAGCGC ACTTCTGTCT GAAGGTCTTC
    AGGGCTGAGG ACTTACCACA GATGGACGAT GCCGTGGTGG ACAGCGTGAA GCAGATCTTC
    GGCTTTGACA GTAACAAGAA GAACCTGGTG GATCCCTTCG TCGAGGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ATGGCCAACC CTCAGTGGAA CCAGAGCATC
    ACGCTGCCTG TCATGTTTCC CTCCATGTCT GAAAAAATGA GGATTCGTGT CATAGACTGG
    GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAATATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGC AGTGGACGAC CACCTGGGCT TCCTCCCCAC CTTCGGGCCC
    TGCTATGTCA ACCTCTACGG CAGCCCCAGG GAGTTCACTG GCTTCCCAGA CCCCTATGCA
    GAGCTCAACA CGGGCAAGGG AGAAGGCGTG GCCTACCGAG GCCGGCTTCT GGTCTCCCTG
    GAGACCAAAC TGGTGGAGCA AGGCGAGCAG AAGGTGGAGG ATCTCGCTGC AGATGACATC
    CTTCGGGTGG AGAAGTACCT CCGGAGGCGC AAGTACTCCC TCTTTGCTGC CTTCTACTCG
    GCCTGCATGC TGCAGGATGT CGACGATGCC ATCCAGTTCG AGGTCAGCAT CGGAAACTAC
    GGGAACAAGT TTGACACGAC CTGCCTGCCG CTGGCCTCCA CCACCCAGTA CAGCCGGGCG
    GTCTTTGACG GGTGCCACTA CTACTACTTA CCCTGGGGCA ACGTGAAGCC TGTGGTGGTG
    CTGTCATCCT ACTGGGAAGA CATCAGCCAC AGAGTCGACA CTCAGAACCA GCTGCTCCGG
    ATCGCCGACC GGCTGGAAGC CGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCGGTGC
    TCCACCGAGG ATGTGGACTC CCTGGTGGCT CAGCTGATGG ATGAGCTCAT TGCAGACTGC
    AGCCAGCCCC TGTGCAACGT CCAAGAGACG CCCTCTGCCA CCCACCTGGA CCAGTACCTG
    TACCGGCTGC GCACCCGTCA CCTGAGCCAG ATCAGTGAGG CGGCCCTGGC CTTGAAGCTC
    GGCCACAGTG AGCTCCCCGC CGCCCTTGAG CAGGCTGAGG ACTGGCTCCT GCGTCTCCGT
    GCCCTGGCGG AGGAGCCCCA GAACAGCCTG CCTGACGTGG TCATCTGGAT GCTGCAGGGA
    GACAAGCGGG TGGCGTACCA GCGGGTGCCC GCCCACGAGG TCCTCTTCTC CCGGCGGGGC
    GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTGCAGACGA TCTTTCTGAA GTATCCAATG
    GAAGGGGTGC CGGGAGCCCG GATGCCGGTG CAGATACGGG TGAAGCTCTG GTTCGGGCTC
    TCTGTGGATG AGAAGGAGTT CAATCAGTTT GCTGAGGGCA AGCTCTCTGT CTTCGCTGAA
    ACCTACGAGA ACCAGACCAA GCTGGCCCTA GTCGGGAACT GGGGCACAAC GGGCCTCACC
    TACCCCAAGT TTTCTGACGT CACAGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC
    TCGGCCGGCT GGGCCTGGGC TGGAGAGTGG TTCGTGTGTC CGGAGAAGAC CCTACTCCAC
    GACACCGATG CGGGCCACCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTG
    CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACAG ATGTGAACGG GGAGAAGGTG
    CTTCCCAAGG ATGACATCGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA AGAATGGTCC
    ACAGACCTCA ATCGGGCCGT TGACGAGCAA GGCTGGGAGT ACAGCATCAC CATCCCTCCG
    GAACGGAAGC CAAGGCACTG GGTCCCTGCC GAGAAGATGT ACTACACACA CCGTCGGCGG
    CGCTGGGTCC GCCTGCGCAG GAGGGACCTC AGCCAGATGG AGGCGCTGAA GAGGCACAGG
    CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCGC TCTTCGGCTG GAAGTTCCAC
    CTGGAGTACC GCAAGACCGA TGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCA
    CTGGAGAAGA CAGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCCCTGGG CGGCGTGGTG
    GACGACAGGA GTGAAGATTC GGTGTCCGTC TCCACCTTGA GCTTCGGCGT GAACAGACCC
    ACGATTTCCT GCATCTTCGA CTACGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG
    GCCCGGGACC TGCCCGCCAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC AATCGTCTCC
    TTCCTGCACC AGAGCCAGAA GACAGTGGTG GTGAAGAACA CCCTGAACCC CACCTGGGAC
    CAGACACTCA TCTTCTATGA GATCGAGATC TTTGGTGAGC CGCCCAGCAT CGCCGAGCAG
    CCGCCCAGCA TCGTGGTGGA GCTATACGAC CATGACACCT ACGGTGCAGA TGAGTTTATG
    GGACGCTGCA TCTGTCAGCC GACTCTGGAA CGGATGCCCC GGCTGACCTG GTTCCCACTG
    ACTAGGGGCA GCCAGCCGGC GGGCGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG
    AAGCCGGCCA TCTACCACAT TCCTGGTTTT GAGGTGCAGG ATACATCAGG GATCCTGGAA
    GAGTCGGAGG ACACAGACCT GCCCTACCCG CCACCCCAGA GAGAGGCCAA CATCTACATG
    GTTCCCCAGA ACATCAAGCC TGCTCTCCAG CGGACGGCCA TCGAGATCCT GGCATGGGGC
    CTGCGGAACA TGAAGAGTTA CCACCTGGCC AGTGTCTCCT CCCCCAGCCT CGTGGTGGAG
    TGCGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCAGAAGAA CCCCAACTTC
    GACGTCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGTCCCCCC
    ATCGTGGTCA AGGTCATCGA TAACTGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT
    ACCATCTGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CAGAGGAGAG TCCGTCCCCA
    CAGGGCGGCC CAGATGATGT GAGCTTACTC AGTCCTGGGG AAGATGTGCT CATTGACATT
    GATGACAAGG AGCCCCTCAT CCCTATGCAG CTTGCAGATG GTCTGTCGGG CTCGGCCCCC
    ACTAACATGG CATCTTCTCC GTCCAGTCCT CATGAGGAGG AGTTCATCGA TTGGTGGAGC
    AAATTCTTTG CCTCGATAGG GGAGAGGGAA AAGTGCGGGT CCTACTTGGA GAAGGACTTT
    GACACTCTAA AGGTCTATGA CACACAACTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT
    GACTTTTGTA ACACCTTCAA GCTCTACCGG GGCAAGACTC AGGAGGAGAT GGAAGACCCA
    TCTGTCGTTG GGGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCGGCC
    ATCCCCATGC CCCCAAGACA GTTCCACCAG CTAGCTGCCC AGGGACCCCA GGAGTGCCTG
    GTCCGCATCT ACATCATCCG GGCATTCGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT
    GACCCTTACA TCAAGATCTC CATAGGGAAG AAATCCGTGA GCGACCAGGA TAACTACATC
    CCCTGCACTC TGGAGCCTGT GTTTGGAAAG ATGTTCGAAC TGACCTGCAC TCTGCCTCTG
    GAGAAGGACC TGAAGATCAC TCTCTATGAC TATGACCTCC TCTCCAAGGA CGAGATGATC
    GGGGAGACGG TCATCGACCT GGAGAATAGG CTGCTGTCCA AGTTTGGAGC TCGCTGCGGA
    CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGA GGGACCAGCT CCGCCCCTCC
    CAGCTTCTCC ACCTGTTCTG CCAGCAGCAG AGAGTCAAGG CCCCCGTGTA CCAGACCGAC
    CGTGTGGTGT TTGGGAATAA AGAATACACG ATTGAAGAAA TAGAGGCTGG GAGGGTCCTG
    AATCCACACC TGGGCCCAGT GGAAGAGCGC CTGGCCTTGC ACGTCCTCCA GCAGCAGGGC
    TTGGTGCCCG AGCATGTGGA GTCCCGGCCC CTCTATAGCC CCCTGCAGCC GGACATTGAG
    CAGGGGAAGC TGCAGATGTG GATCGACCTG TTTCCAAAGG CCCTGGGGCG GCCTGGACCT
    CCCTTCAACA TCACTCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGCAT TATCTGGAAC
    ACCAAGGATG TGATCCTGGA CGACCTCAGC ATCACAGGAG AGAAGATGAG CGACATTTAT
    GTGAAAGGTT GGATGGTTGG CTTTGAAGAA CACAAGCAAA AGACAGATGT GCATTATCGG
    TCCCTGGGAG GCGAAGGGAA CTTTAACTGG AGGTTCATTT TCCCCTTTGA CTACCTGCTG
    GCTGAGCAAG TCTGCACCGT CTCCAAGAAG GATGCTTTCT GGAAGCTGGA CAAGACCGAG
    AGCAAAATCC CAGCACGAGT GGTATTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT
    GATTTCCTGG GCTCCTTGCA GCTGGATCTC AACCGCATGC CCAAGCCTGC CAAGACAGCC
    GAGAAGTGCT CCCTGGACCA GCTGGATGAC ACTTTCCACC CAGAGTGGTT TGTGTCCCTT
    TTTGAACAGA AAACTGTGAA GGGCTGGTGG CCCTGTGTGG CGGAAGAGGG TGAGAAGAAG
    AAGCTGGCGG GCAAGCTGGA GATGACCTTG GAGATTGTGG CGGAGAGTGA GCATGAGGAG
    CGGCCCGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CCAAGCTCGA GGACCCAAGG
    CGCCCTGACA CCTCCTTCCT CTGGTTCACC TCCCCGTACA AGACTATGAA GTTCATCCTG
    TGGCGGCGTT TCCGGTGTGC CATCATCCTC TTCATCATCC TCTTCATCCT ACTGCTGTTC
    TTGGGCATCT TCGTTTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb49781
    Clone ID Related Accession (Same CDS sequence) XM_023618913.1
    Accession Version XM_023618913.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6357bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product dysferlin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009158.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGTGCT GCCTGCTAGT GAAGGCCAGC AACCTTCCCA CTGTGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAA
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG GGTCCCCCTG
    GACCAGAGCT CTGAGCTTCA CGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
    TTCCTGGGAG AAGCCAAGGT CCCACTCCGG GAGGTCCTTG CCACCCCCAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACGCCTCT CCCTGGAGCT GTGCCCCTGT TCCCACCCTC TGTGCCGCTG
    GAACCCTCCC CGACTCTGCC CGATGTGGAT ATGCTGGCTG ATACAGGAGG AGAGGAGGAC
    ACCGAGGACC AGGGGCTCAC GGGAGATGAG GCAGAGCCAT TCTTGGATCA GAACGGAGCC
    CCAGGCCCCG GGGCTCCCAC CACCCTGAAG AAGCCACCTT CCCATCCTCC CCCCTACCAT
    CCTGGGGGGA AAAGGAAGAG AAGCACGCCT GCGCCCAGAA AGCTGCTTTC GGATAAACCA
    CAGGACTTCC AGATCAGGGT CCAGGTGATC GAGGGGCGCC AGCTGCCAGG GGTGAACATC
    AAGCCTGTGG TCAAGGTCAC CGCGGCCAGG CAGACCAAGC GGACTCGGAT TCACAGGGGA
    AACAGCCCGC TCTTCAACGA GACTCTCTTC TTCAACGTGT TTGACTCTCC CTCGGAGCTG
    TTTGACGAGG CCGTCTTTAT CACGGTGGTA GACTCCTGTT CGCTCCGGAC AGATGCCCTC
    ATCGGGGAGT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA CGCCTATCTC
    AGGAAGTGGC TGCTGCTCTC GGACCCTGAC GATTTCTCTG CTGGGCCCAA AGGCTACCTG
    AAAGCAAGCC TTTGTGTGCT GGGGCCTGGA GACGAAGCTC CGCTGGAGAG AAAGGACCCC
    TCTGAAGACA AGGAGGACAT TGAAAGCAAT CTGCTCAGGC CAACTGGCAT GGCCCTTCGA
    GGAGCGCACT TCTGTCTGAA GGTCTTCAGG GCTGAGGACT TACCACAGAT GGACGATGCC
    GTGGTGGACA GCGTGAAGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CCTGGTGGAT
    CCCTTCGTCG AGGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGATG
    GCCAACCCTC AGTGGAACCA GAGCATCACG CTGCCTGTCA TGTTTCCCTC CATGTCTGAA
    AAAATGAGGA TTCGTGTCAT AGACTGGGAC CGCCTCACCC ACAATGACAT CGTGGCCACC
    ACCTACCTGA ATATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGCAGA GGAGCCTGCA
    GGTGTGGTCA AGCCTCCTCC AGCCACGGAC CTGGACGACC ACCTGGGCTT CCTCCCCACC
    TTCGGGCCCT GCTATGTCAA CCTCTACGGC AGCCCCAGGG AGTTCACTGG CTTCCCAGAC
    CCCTATGCAG AGCTCAACAC GGGCAAGGGA GAAGGCGTGG CCTACCGAGG CCGGCTTCTG
    GTCTCCCTGG AGACCAAACT GGTGGAGCAA GGCGAGCAGA AGGTGGAGGA TCTCGCTGCA
    GATGACATCC TTCGGGTGGA GAAGTACCTC CGGAGGCGCA AGTACTCCCT CTTTGCTGCC
    TTCTACTCGG CCTGCATGCT GCAGGATGTC GACGATGCCA TCCAGTTCGA GGTCAGCATC
    GGAAACTACG GGAACAAGTT TGACACGACC TGCCTGCCGC TGGCCTCCAC CACCCAGTAC
    AGCCGGGCGG TCTTTGACGG GTGCCACTAC TACTACTTAC CCTGGGGCAA CGTGAAGCCT
    GTGGTGGTGC TGTCATCCTA CTGGGAAGAC ATCAGCCACA GAGTCGACAC TCAGAACCAG
    CTGCTCCGGA TCGCCGACCG GCTGGAAGCC GGCCTGGAGC AGGTCCACCT GGCCCTGAAG
    GCGCGGTGCT CCACCGAGGA TGTGGACTCC CTGGTGGCTC AGCTGATGGA TGAGCTCATT
    GCAGACTGCA GCCAGCCCCT GTGCAACGTC CAAGAGACGC CCTCTGCCAC CCACCTGGAC
    CAGTACCTGT ACCGGCTGCG CACCCGTCAC CTGAGCCAGA TCAGTGAGGC GGCCCTGGCC
    TTGAAGCTCG GCCACAGTGA GCTCCCCGCC GCCCTTGAGC AGGCTGAGGA CTGGCTCCTG
    CGTCTCCGTG CCCTGGCGGA GGAGCCCCAG AACAGCCTGC CTGACGTGGT CATCTGGATG
    CTGCAGGGAG ACAAGCGGGT GGCGTACCAG CGGGTGCCCG CCCACGAGGT CCTCTTCTCC
    CGGCGGGGCG CCAACTACTG TGGCAAGAAT TGTGGGAAGC TGCAGACGAT CTTTCTGAAG
    TATCCAATGG AAGGGGTGCC GGGAGCCCGG ATGCCGGTGC AGATACGGGT GAAGCTCTGG
    TTCGGGCTCT CTGTGGATGA GAAGGAGTTC AATCAGTTTG CTGAGGGCAA GCTCTCTGTC
    TTCGCTGAAA CCTACGAGAA CCAGACCAAG CTGGCCCTAG TCGGGAACTG GGGCACAACG
    GGCCTCACCT ACCCCAAGTT TTCTGACGTC ACAGGCAAGA TCAAGCTACC CAAGGACAGC
    TTCCGCCCCT CGGCCGGCTG GGCCTGGGCT GGAGAGTGGT TCGTGTGTCC GGAGAAGACC
    CTACTCCACG ACACCGATGC GGGCCACCTG AGCTTTGTGG AGGAGGTGTT TGAGAACCAG
    ACCCGGCTGC CCGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACAGA TGTGAACGGG
    GAGAAGGTGC TTCCCAAGGA TGACATCGAG TGCCCACTGG GCTGGAAGTG GGAAGATGAA
    GAATGGTCCA CAGACCTCAA TCGGGCCGTT GACGAGCAAG GCTGGGAGTA CAGCATCACC
    ATCCCTCCGG AACGGAAGCC AAGGCACTGG GTCCCTGCCG AGAAGATGTA CTACACACAC
    CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG AGGGACCTCA GCCAGATGGA GGCGCTGAAG
    AGGCACAGGC AGGCGGAGGC GGAGGGCGAG GGCTGGGAGT ACGCCTCGCT CTTCGGCTGG
    AAGTTCCACC TGGAGTACCG CAAGACCGAT GCCTTCCGCC GCCGCCGCTG GCGCCGCCGC
    ATGGAGCCAC TGGAGAAGAC AGGGCCTGCA GCTGTGTTTG CCCTCGAGGG GGCCCTGGGC
    GGCGTGGTGG ACGACAGGAG TGAAGATTCG GTGTCCGTCT CCACCTTGAG CTTCGGCGTG
    AACAGACCCA CGATTTCCTG CATCTTCGAC TACGGGAACC GCTACCATCT ACGCTGCTAC
    ATGTACCAGG CCCGGGACCT GCCCGCCATG GACAAGGACT CTTTTTCTGA TCCCTACGCA
    ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG TGAAGAACAC CCTGAACCCC
    ACCTGGGACC AGACACTCAT CTTCTATGAG ATCGAGATCT TTGGTGAGCC GCCCAGCATC
    GCCGAGCAGC CGCCCAGCAT CGTGGTGGAG CTATACGACC ATGACACCTA CGGTGCAGAT
    GAGTTTATGG GACGCTGCAT CTGTCAGCCG ACTCTGGAAC GGATGCCCCG GCTGACCTGG
    TTCCCACTGA CTAGGGGCAG CCAGCCGGCG GGCGAGCTGC TGGCCTCTTT TGAGCTCATC
    CAGAGAGAGA AGCCGGCCAT CTACCACATT CCTGGTTTTG AGGTGCAGGA TACATCAGGG
    ATCCTGGAAG AGCTGTGTGC ACCTGCCTTC TTCCTCCAGG GCCTCCTCCA AACGTCGGAG
    GACACAGACC TGCCCTACCC GCCACCCCAG AGAGAGGCCA ACATCTACAT GGTTCCCCAG
    AACATCAAGC CTGCTCTCCA GCGGACGGCC ATCGAGATCC TGGCATGGGG CCTGCGGAAC
    ATGAAGAGTT ACCACCTGGC CAGTGTCTCC TCCCCCAGCC TCGTGGTGGA GTGCGGGGGC
    CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCAGAAGA ACCCCAACTT CGACGTCTGC
    ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGTCCCCC CATCGTGGTC
    AAGGTCATCG ATAACTGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCTGC
    TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCAGAGGAGA GTCCGTCCCC ACAGGGCGGC
    CCAGATGATG TGAGCTTACT CAGTCCTGGG GAAGATGTGC TCATTGACAT TGATGACAAG
    GAGCCCCTCA TCCCTATGCA GCTTGCAGAT GGTCTGTCGG GCTCGGCCCC CACTAACATG
    GCATCTTCTC CGTCCAGTCC TCATGAGGAG GAGTTCATCG ATTGGTGGAG CAAATTCTTT
    GCCTCGATAG GGGAGAGGGA AAAGTGCGGG TCCTACTTGG AGAAGGACTT TGACACTCTA
    AAGGTCTATG ACACACAACT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT
    AACACCTTCA AGCTCTACCG GGGCAAGACT CAGGAGGAGA TGGAAGACCC ATCTGTCGTT
    GGGGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCGGC CATCCCCATG
    CCCCCAAGAC AGTTCCACCA GCTAGCTGCC CAGGGACCCC AGGAGTGCCT GGTCCGCATC
    TACATCATCC GGGCATTCGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGACCCTTAC
    ATCAAGATCT CCATAGGGAA GAAATCCGTG AGCGACCAGG ATAACTACAT CCCCTGCACT
    CTGGAGCCTG TGTTTGGAAA GATGTTCGAA CTGACCTGCA CTCTGCCTCT GGAGAAGGAC
    CTGAAGATCA CTCTCTATGA CTATGACCTC CTCTCCAAGG ACGAGATGAT CGGGGAGACG
    GTCATCGACC TGGAGAATAG GCTGCTGTCC AAGTTTGGAG CTCGCTGCGG ACTCCCACAG
    ACCTACTGTG TCTCTGGACC GAACCAGTGG AGGGACCAGC TCCGCCCCTC CCAGCTTCTC
    CACCTGTTCT GCCAGCAGCA GAGAGTCAAG GCCCCCGTGT ACCAGACCGA CCGTGTGGTG
    TTTGGGAATA AAGAATACAC GATTGAAGAA ATAGAGGCTG GGAGGGTCCT GAATCCACAC
    CTGGGCCCAG TGGAAGAGCG CCTGGCCTTG CACGTCCTCC AGCAGCAGGG CTTGGTGCCC
    GAGCATGTGG AGTCCCGGCC CCTCTATAGC CCCCTGCAGC CGGACATTGA GCAGGGGAAG
    CTGCAGATGT GGATCGACCT GTTTCCAAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC
    ATCACTCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGCA TTATCTGGAA CACCAAGGAT
    GTGATCCTGG ACGACCTCAG CATCACAGGA GAGAAGATGA GCGACATTTA TGTGAAAGGT
    TGGATGGTTG GCTTTGAAGA ACACAAGCAA AAGACAGATG TGCATTATCG GTCCCTGGGA
    GGCGAAGGGA ACTTTAACTG GAGGTTCATT TTCCCCTTTG ACTACCTGCT GGCTGAGCAA
    GTCTGCACCG TCTCCAAGAA GGATGCTTTC TGGAAGCTGG ACAAGACCGA GAGCAAAATC
    CCAGCACGAG TGGTATTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTCCTG
    GGCTCCTTGC AGCTGGATCT CAACCGCATG CCCAAGCCTG CCAAGACAGC CGAGAAGTGC
    TCCCTGGACC AGCTGGATGA CACTTTCCAC CCAGAGTGGT TTGTGTCCCT TTTTGAACAG
    AAAACTGTGA AGGGCTGGTG GCCCTGTGTG GCGGAAGAGG GTGAGAAGAA GAAGCTGGCG
    GGCAAGCTGG AGATGACCTT GGAGATTGTG GCGGAGAGTG AGCATGAGGA GCGGCCCGCT
    GGCCAGGGCC GGGATGAGCC CAACATGAAC CCCAAGCTCG AGGACCCAAG GCGCCCTGAC
    ACCTCCTTCC TCTGGTTCAC CTCCCCGTAC AAGACTATGA AGTTCATCCT GTGGCGGCGT
    TTCCGGTGTG CCATCATCCT CTTCATCATC CTCTTCATCC TACTGCTGTT CTTGGGCATC
    TTCGTTTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023474681.1
    CDS211..6567
    Translation

    Target ORF information:

    RefSeq Version XM_023618913.1
    Organism Equus caballus(horse)
    Definition Equus caballus dysferlin (DYSF), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023618913.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGTGCT GCCTGCTAGT GAAGGCCAGC AACCTTCCCA CTGTGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAA
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG GGTCCCCCTG
    GACCAGAGCT CTGAGCTTCA CGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
    TTCCTGGGAG AAGCCAAGGT CCCACTCCGG GAGGTCCTTG CCACCCCCAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACGCCTCT CCCTGGAGCT GTGCCCCTGT TCCCACCCTC TGTGCCGCTG
    GAACCCTCCC CGACTCTGCC CGATGTGGAT ATGCTGGCTG ATACAGGAGG AGAGGAGGAC
    ACCGAGGACC AGGGGCTCAC GGGAGATGAG GCAGAGCCAT TCTTGGATCA GAACGGAGCC
    CCAGGCCCCG GGGCTCCCAC CACCCTGAAG AAGCCACCTT CCCATCCTCC CCCCTACCAT
    CCTGGGGGGA AAAGGAAGAG AAGCACGCCT GCGCCCAGAA AGCTGCTTTC GGATAAACCA
    CAGGACTTCC AGATCAGGGT CCAGGTGATC GAGGGGCGCC AGCTGCCAGG GGTGAACATC
    AAGCCTGTGG TCAAGGTCAC CGCGGCCAGG CAGACCAAGC GGACTCGGAT TCACAGGGGA
    AACAGCCCGC TCTTCAACGA GACTCTCTTC TTCAACGTGT TTGACTCTCC CTCGGAGCTG
    TTTGACGAGG CCGTCTTTAT CACGGTGGTA GACTCCTGTT CGCTCCGGAC AGATGCCCTC
    ATCGGGGAGT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA CGCCTATCTC
    AGGAAGTGGC TGCTGCTCTC GGACCCTGAC GATTTCTCTG CTGGGCCCAA AGGCTACCTG
    AAAGCAAGCC TTTGTGTGCT GGGGCCTGGA GACGAAGCTC CGCTGGAGAG AAAGGACCCC
    TCTGAAGACA AGGAGGACAT TGAAAGCAAT CTGCTCAGGC CAACTGGCAT GGCCCTTCGA
    GGAGCGCACT TCTGTCTGAA GGTCTTCAGG GCTGAGGACT TACCACAGAT GGACGATGCC
    GTGGTGGACA GCGTGAAGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CCTGGTGGAT
    CCCTTCGTCG AGGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGATG
    GCCAACCCTC AGTGGAACCA GAGCATCACG CTGCCTGTCA TGTTTCCCTC CATGTCTGAA
    AAAATGAGGA TTCGTGTCAT AGACTGGGAC CGCCTCACCC ACAATGACAT CGTGGCCACC
    ACCTACCTGA ATATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGCAGA GGAGCCTGCA
    GGTGTGGTCA AGCCTCCTCC AGCCACGGAC CTGGACGACC ACCTGGGCTT CCTCCCCACC
    TTCGGGCCCT GCTATGTCAA CCTCTACGGC AGCCCCAGGG AGTTCACTGG CTTCCCAGAC
    CCCTATGCAG AGCTCAACAC GGGCAAGGGA GAAGGCGTGG CCTACCGAGG CCGGCTTCTG
    GTCTCCCTGG AGACCAAACT GGTGGAGCAA GGCGAGCAGA AGGTGGAGGA TCTCGCTGCA
    GATGACATCC TTCGGGTGGA GAAGTACCTC CGGAGGCGCA AGTACTCCCT CTTTGCTGCC
    TTCTACTCGG CCTGCATGCT GCAGGATGTC GACGATGCCA TCCAGTTCGA GGTCAGCATC
    GGAAACTACG GGAACAAGTT TGACACGACC TGCCTGCCGC TGGCCTCCAC CACCCAGTAC
    AGCCGGGCGG TCTTTGACGG GTGCCACTAC TACTACTTAC CCTGGGGCAA CGTGAAGCCT
    GTGGTGGTGC TGTCATCCTA CTGGGAAGAC ATCAGCCACA GAGTCGACAC TCAGAACCAG
    CTGCTCCGGA TCGCCGACCG GCTGGAAGCC GGCCTGGAGC AGGTCCACCT GGCCCTGAAG
    GCGCGGTGCT CCACCGAGGA TGTGGACTCC CTGGTGGCTC AGCTGATGGA TGAGCTCATT
    GCAGACTGCA GCCAGCCCCT GTGCAACGTC CAAGAGACGC CCTCTGCCAC CCACCTGGAC
    CAGTACCTGT ACCGGCTGCG CACCCGTCAC CTGAGCCAGA TCAGTGAGGC GGCCCTGGCC
    TTGAAGCTCG GCCACAGTGA GCTCCCCGCC GCCCTTGAGC AGGCTGAGGA CTGGCTCCTG
    CGTCTCCGTG CCCTGGCGGA GGAGCCCCAG AACAGCCTGC CTGACGTGGT CATCTGGATG
    CTGCAGGGAG ACAAGCGGGT GGCGTACCAG CGGGTGCCCG CCCACGAGGT CCTCTTCTCC
    CGGCGGGGCG CCAACTACTG TGGCAAGAAT TGTGGGAAGC TGCAGACGAT CTTTCTGAAG
    TATCCAATGG AAGGGGTGCC GGGAGCCCGG ATGCCGGTGC AGATACGGGT GAAGCTCTGG
    TTCGGGCTCT CTGTGGATGA GAAGGAGTTC AATCAGTTTG CTGAGGGCAA GCTCTCTGTC
    TTCGCTGAAA CCTACGAGAA CCAGACCAAG CTGGCCCTAG TCGGGAACTG GGGCACAACG
    GGCCTCACCT ACCCCAAGTT TTCTGACGTC ACAGGCAAGA TCAAGCTACC CAAGGACAGC
    TTCCGCCCCT CGGCCGGCTG GGCCTGGGCT GGAGAGTGGT TCGTGTGTCC GGAGAAGACC
    CTACTCCACG ACACCGATGC GGGCCACCTG AGCTTTGTGG AGGAGGTGTT TGAGAACCAG
    ACCCGGCTGC CCGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACAGA TGTGAACGGG
    GAGAAGGTGC TTCCCAAGGA TGACATCGAG TGCCCACTGG GCTGGAAGTG GGAAGATGAA
    GAATGGTCCA CAGACCTCAA TCGGGCCGTT GACGAGCAAG GCTGGGAGTA CAGCATCACC
    ATCCCTCCGG AACGGAAGCC AAGGCACTGG GTCCCTGCCG AGAAGATGTA CTACACACAC
    CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG AGGGACCTCA GCCAGATGGA GGCGCTGAAG
    AGGCACAGGC AGGCGGAGGC GGAGGGCGAG GGCTGGGAGT ACGCCTCGCT CTTCGGCTGG
    AAGTTCCACC TGGAGTACCG CAAGACCGAT GCCTTCCGCC GCCGCCGCTG GCGCCGCCGC
    ATGGAGCCAC TGGAGAAGAC AGGGCCTGCA GCTGTGTTTG CCCTCGAGGG GGCCCTGGGC
    GGCGTGGTGG ACGACAGGAG TGAAGATTCG GTGTCCGTCT CCACCTTGAG CTTCGGCGTG
    AACAGACCCA CGATTTCCTG CATCTTCGAC TACGGGAACC GCTACCATCT ACGCTGCTAC
    ATGTACCAGG CCCGGGACCT GCCCGCCATG GACAAGGACT CTTTTTCTGA TCCCTACGCA
    ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG TGAAGAACAC CCTGAACCCC
    ACCTGGGACC AGACACTCAT CTTCTATGAG ATCGAGATCT TTGGTGAGCC GCCCAGCATC
    GCCGAGCAGC CGCCCAGCAT CGTGGTGGAG CTATACGACC ATGACACCTA CGGTGCAGAT
    GAGTTTATGG GACGCTGCAT CTGTCAGCCG ACTCTGGAAC GGATGCCCCG GCTGACCTGG
    TTCCCACTGA CTAGGGGCAG CCAGCCGGCG GGCGAGCTGC TGGCCTCTTT TGAGCTCATC
    CAGAGAGAGA AGCCGGCCAT CTACCACATT CCTGGTTTTG AGGTGCAGGA TACATCAGGG
    ATCCTGGAAG AGCTGTGTGC ACCTGCCTTC TTCCTCCAGG GCCTCCTCCA AACGTCGGAG
    GACACAGACC TGCCCTACCC GCCACCCCAG AGAGAGGCCA ACATCTACAT GGTTCCCCAG
    AACATCAAGC CTGCTCTCCA GCGGACGGCC ATCGAGATCC TGGCATGGGG CCTGCGGAAC
    ATGAAGAGTT ACCACCTGGC CAGTGTCTCC TCCCCCAGCC TCGTGGTGGA GTGCGGGGGC
    CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCAGAAGA ACCCCAACTT CGACGTCTGC
    ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGTCCCCC CATCGTGGTC
    AAGGTCATCG ATAACTGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCTGC
    TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCAGAGGAGA GTCCGTCCCC ACAGGGCGGC
    CCAGATGATG TGAGCTTACT CAGTCCTGGG GAAGATGTGC TCATTGACAT TGATGACAAG
    GAGCCCCTCA TCCCTATGCA GCTTGCAGAT GGTCTGTCGG GCTCGGCCCC CACTAACATG
    GCATCTTCTC CGTCCAGTCC TCATGAGGAG GAGTTCATCG ATTGGTGGAG CAAATTCTTT
    GCCTCGATAG GGGAGAGGGA AAAGTGCGGG TCCTACTTGG AGAAGGACTT TGACACTCTA
    AAGGTCTATG ACACACAACT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT
    AACACCTTCA AGCTCTACCG GGGCAAGACT CAGGAGGAGA TGGAAGACCC ATCTGTCGTT
    GGGGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCGGC CATCCCCATG
    CCCCCAAGAC AGTTCCACCA GCTAGCTGCC CAGGGACCCC AGGAGTGCCT GGTCCGCATC
    TACATCATCC GGGCATTCGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGACCCTTAC
    ATCAAGATCT CCATAGGGAA GAAATCCGTG AGCGACCAGG ATAACTACAT CCCCTGCACT
    CTGGAGCCTG TGTTTGGAAA GATGTTCGAA CTGACCTGCA CTCTGCCTCT GGAGAAGGAC
    CTGAAGATCA CTCTCTATGA CTATGACCTC CTCTCCAAGG ACGAGATGAT CGGGGAGACG
    GTCATCGACC TGGAGAATAG GCTGCTGTCC AAGTTTGGAG CTCGCTGCGG ACTCCCACAG
    ACCTACTGTG TCTCTGGACC GAACCAGTGG AGGGACCAGC TCCGCCCCTC CCAGCTTCTC
    CACCTGTTCT GCCAGCAGCA GAGAGTCAAG GCCCCCGTGT ACCAGACCGA CCGTGTGGTG
    TTTGGGAATA AAGAATACAC GATTGAAGAA ATAGAGGCTG GGAGGGTCCT GAATCCACAC
    CTGGGCCCAG TGGAAGAGCG CCTGGCCTTG CACGTCCTCC AGCAGCAGGG CTTGGTGCCC
    GAGCATGTGG AGTCCCGGCC CCTCTATAGC CCCCTGCAGC CGGACATTGA GCAGGGGAAG
    CTGCAGATGT GGATCGACCT GTTTCCAAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC
    ATCACTCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGCA TTATCTGGAA CACCAAGGAT
    GTGATCCTGG ACGACCTCAG CATCACAGGA GAGAAGATGA GCGACATTTA TGTGAAAGGT
    TGGATGGTTG GCTTTGAAGA ACACAAGCAA AAGACAGATG TGCATTATCG GTCCCTGGGA
    GGCGAAGGGA ACTTTAACTG GAGGTTCATT TTCCCCTTTG ACTACCTGCT GGCTGAGCAA
    GTCTGCACCG TCTCCAAGAA GGATGCTTTC TGGAAGCTGG ACAAGACCGA GAGCAAAATC
    CCAGCACGAG TGGTATTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTCCTG
    GGCTCCTTGC AGCTGGATCT CAACCGCATG CCCAAGCCTG CCAAGACAGC CGAGAAGTGC
    TCCCTGGACC AGCTGGATGA CACTTTCCAC CCAGAGTGGT TTGTGTCCCT TTTTGAACAG
    AAAACTGTGA AGGGCTGGTG GCCCTGTGTG GCGGAAGAGG GTGAGAAGAA GAAGCTGGCG
    GGCAAGCTGG AGATGACCTT GGAGATTGTG GCGGAGAGTG AGCATGAGGA GCGGCCCGCT
    GGCCAGGGCC GGGATGAGCC CAACATGAAC CCCAAGCTCG AGGACCCAAG GCGCCCTGAC
    ACCTCCTTCC TCTGGTTCAC CTCCCCGTAC AAGACTATGA AGTTCATCCT GTGGCGGCGT
    TTCCGGTGTG CCATCATCCT CTTCATCATC CTCTTCATCC TACTGCTGTT CTTGGGCATC
    TTCGTTTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb49782
    Clone ID Related Accession (Same CDS sequence) XM_023618914.1
    Accession Version XM_023618914.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6345bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product dysferlin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009158.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGTGCT GCCTGCTAGT GAAGGCCAGC AACCTTCCCA CTGTGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAA
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG GGTCCCCCTG
    GACCAGAGCT CTGAGCTTCA CGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
    TTCCTGGGAG AAGCCAAGGT CCCACTCCGG GAGGTCCTTG CCACCCCCAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACGCCTCT CCCTGGAGCT GTGCCCCTGT TCCCACCCTC TGTGCCGCTG
    GAACCCTCCC CGACTCTGCC CGATGTGGAT ATGCTGGCTG GTGGGGGACA GAGCCGGGCC
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAATGG
    CAGGCCCCCA CGGATACAGG AGGAGAGGAG GACACCGAGG ACCAGGGGCT CACGGGAGAT
    GAGGCAGAGC CATTCTTGGA TCAGAACGGA GCCCCAGGCC CCGGGGCTCC CACCACCCTG
    AAGAAGCCAC CTTCCCATCC TCCCCCCTAC CATCCTGGGG GGAAAAGGAA GAGAAGCACG
    CCTGCGCCCA GAAAGCTGCT TTCGGATAAA CCACAGGACT TCCAGATCAG GGTCCAGGTG
    ATCGAGGGGC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCGGCC
    AGGCAGACCA AGCGGACTCG GATTCACAGG GGAAACAGCC CGCTCTTCAA CGAGACTCTC
    TTCTTCAACG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGGCCGTCTT TATCACGGTG
    GTAGACTCCT GTTCGCTCCG GACAGATGCC CTCATCGGGG AGTTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCT
    GACGATTTCT CTGCTGGGCC CAAAGGCTAC CTGAAAGCAA GCCTTTGTGT GCTGGGGCCT
    GGAGACGAAG CTCCGCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAGC
    AATCTGCTCA GGCCAACTGG CATGGCCCTT CGAGGAGCGC ACTTCTGTCT GAAGGTCTTC
    AGGGCTGAGG ACTTACCACA GATGGACGAT GCCGTGGTGG ACAGCGTGAA GCAGATCTTC
    GGCTTTGACA GTAACAAGAA GAACCTGGTG GATCCCTTCG TCGAGGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ATGGCCAACC CTCAGTGGAA CCAGAGCATC
    ACGCTGCCTG TCATGTTTCC CTCCATGTCT GAAAAAATGA GGATTCGTGT CATAGACTGG
    GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAATATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGC AGAGGAGCCT GCAGGTGTGG TCAAGCCTCC TCCAGCCACG
    GACCTGGACG ACCACCTGGG CTTCCTCCCC ACCTTCGGGC CCTGCTATGT CAACCTCTAC
    GGCAGCCCCA GGGAGTTCAC TGGCTTCCCA GACCCCTATG CAGAGCTCAA CACGGGCAAG
    GGAGAAGGCG TGGCCTACCG AGGCCGGCTT CTGGTCTCCC TGGAGACCAA ACTGGTGGAG
    CAAGGCGAGC AGAAGGTGGA GGATCTCGCT GCAGATGACA TCCTTCGGGT GGAGAAGTAC
    CTCCGGAGGC GCAAGTACTC CCTCTTTGCT GCCTTCTACT CGGCCTGCAT GCTGCAGGAT
    GTCGACGATG CCATCCAGTT CGAGGTCAGC ATCGGAAACT ACGGGAACAA GTTTGACACG
    ACCTGCCTGC CGCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA CGGGTGCCAC
    TACTACTACT TACCCTGGGG CAACGTGAAG CCTGTGGTGG TGCTGTCATC CTACTGGGAA
    GACATCAGCC ACAGAGTCGA CACTCAGAAC CAGCTGCTCC GGATCGCCGA CCGGCTGGAA
    GCCGGCCTGG AGCAGGTCCA CCTGGCCCTG AAGGCGCGGT GCTCCACCGA GGATGTGGAC
    TCCCTGGTGG CTCAGCTGAT GGATGAGCTC ATTGCAGACT GCAGCCAGCC CCTGTGCAAC
    GTCCAAGAGA CGCCCTCTGC CACCCACCTG GACCAGTACC TGTACCGGCT GCGCACCCGT
    CACCTGAGCC AGATCAGTGA GGCGGCCCTG GCCTTGAAGC TCGGCCACAG TGAGCTCCCC
    GCCGCCCTTG AGCAGGCTGA GGACTGGCTC CTGCGTCTCC GTGCCCTGGC GGAGGAGCCC
    CAGAACAGCC TGCCTGACGT GGTCATCTGG ATGCTGCAGG GAGACAAGCG GGTGGCGTAC
    CAGCGGGTGC CCGCCCACGA GGTCCTCTTC TCCCGGCGGG GCGCCAACTA CTGTGGCAAG
    AATTGTGGGA AGCTGCAGAC GATCTTTCTG AAGTATCCAA TGGAAGGGGT GCCGGGAGCC
    CGGATGCCGG TGCAGATACG GGTGAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAG
    TTCAATCAGT TTGCTGAGGG CAAGCTCTCT GTCTTCGCTG AAACCTACGA GAACCAGACC
    AAGCTGGCCC TAGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAC
    GTCACAGGCA AGATCAAGCT ACCCAAGGAC AGCTTCCGCC CCTCGGCCGG CTGGGCCTGG
    GCTGGAGAGT GGTTCGTGTG TCCGGAGAAG ACCCTACTCC ACGACACCGA TGCGGGCCAC
    CTGAGCTTTG TGGAGGAGGT GTTTGAGAAC CAGACCCGGC TGCCCGGAGG CCAGTGGATC
    TACATGAGTG ACAACTACAC AGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC
    GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCC
    GTTGACGAGC AAGGCTGGGA GTACAGCATC ACCATCCCTC CGGAACGGAA GCCAAGGCAC
    TGGGTCCCTG CCGAGAAGAT GTACTACACA CACCGTCGGC GGCGCTGGGT CCGCCTGCGC
    AGGAGGGACC TCAGCCAGAT GGAGGCGCTG AAGAGGCACA GGCAGGCGGA GGCGGAGGGC
    GAGGGCTGGG AGTACGCCTC GCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACC
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CACTGGAGAA GACAGGGCCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCGTGG TGGACGACAG GAGTGAAGAT
    TCGGTGTCCG TCTCCACCTT GAGCTTCGGC GTGAACAGAC CCACGATTTC CTGCATCTTC
    GACTACGGGA ACCGCTACCA TCTACGCTGC TACATGTACC AGGCCCGGGA CCTGCCCGCC
    ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCAATCGTCT CCTTCCTGCA CCAGAGCCAG
    AAGACAGTGG TGGTGAAGAA CACCCTGAAC CCCACCTGGG ACCAGACACT CATCTTCTAT
    GAGATCGAGA TCTTTGGTGA GCCGCCCAGC ATCGCCGAGC AGCCGCCCAG CATCGTGGTG
    GAGCTATACG ACCATGACAC CTACGGTGCA GATGAGTTTA TGGGACGCTG CATCTGTCAG
    CCGACTCTGG AACGGATGCC CCGGCTGACC TGGTTCCCAC TGACTAGGGG CAGCCAGCCG
    GCGGGCGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCGGC CATCTACCAC
    ATTCCTGGTT TTGAGGTGCA GGATACATCA GGGATCCTGG AAGAGTCGGA GGACACAGAC
    CTGCCCTACC CGCCACCCCA GAGAGAGGCC AACATCTACA TGGTTCCCCA GAACATCAAG
    CCTGCTCTCC AGCGGACGGC CATCGAGATC CTGGCATGGG GCCTGCGGAA CATGAAGAGT
    TACCACCTGG CCAGTGTCTC CTCCCCCAGC CTCGTGGTGG AGTGCGGGGG CCAGACGGTG
    CAGTCCTGTG TCATCAGGAA CCTCCAGAAG AACCCCAACT TCGACGTCTG CACCCTCTTC
    ATGGAAGTGA TGCTGCCCAG GGAGGAGCTC TACTGTCCCC CCATCGTGGT CAAGGTCATC
    GATAACTGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCTG CTCCCTGGAG
    AGCTTCCTGT GTGACCCCTA CTCAGAGGAG AGTCCGTCCC CACAGGGCGG CCCAGATGAT
    GTGAGCTTAC TCAGTCCTGG GGAAGATGTG CTCATTGACA TTGATGACAA GGAGCCCCTC
    ATCCCTATGC AGGAGGAGGA GTTCATCGAT TGGTGGAGCA AATTCTTTGC CTCGATAGGG
    GAGAGGGAAA AGTGCGGGTC CTACTTGGAG AAGGACTTTG ACACTCTAAA GGTCTATGAC
    ACACAACTGG AGAATGTGGA GGCCTTTGAG GGCCTGTCTG ACTTTTGTAA CACCTTCAAG
    CTCTACCGGG GCAAGACTCA GGAGGAGATG GAAGACCCAT CTGTCGTTGG GGAATTTAAG
    GGCCTCTTCA AAATTTATCC CCTCCCAGAA GACCCGGCCA TCCCCATGCC CCCAAGACAG
    TTCCACCAGC TAGCTGCCCA GGGACCCCAG GAGTGCCTGG TCCGCATCTA CATCATCCGG
    GCATTCGGCC TGCAGCCCAA GGACCCCAAT GGGAAGTGTG ACCCTTACAT CAAGATCTCC
    ATAGGGAAGA AATCCGTGAG CGACCAGGAT AACTACATCC CCTGCACTCT GGAGCCTGTG
    TTTGGAAAGA TGTTCGAACT GACCTGCACT CTGCCTCTGG AGAAGGACCT GAAGATCACT
    CTCTATGACT ATGACCTCCT CTCCAAGGAC GAGATGATCG GGGAGACGGT CATCGACCTG
    GAGAATAGGC TGCTGTCCAA GTTTGGAGCT CGCTGCGGAC TCCCACAGAC CTACTGTGTC
    TCTGGACCGA ACCAGTGGAG GGACCAGCTC CGCCCCTCCC AGCTTCTCCA CCTGTTCTGC
    CAGCAGCAGA GAGTCAAGGC CCCCGTGTAC CAGACCGACC GTGTGGTGTT TGGGAATAAA
    GAATACACGA TTGAAGAAAT AGAGGCTGGG AGGGTCCTGA ATCCACACCT GGGCCCAGTG
    GAAGAGCGCC TGGCCTTGCA CGTCCTCCAG CAGCAGGGCT TGGTGCCCGA GCATGTGGAG
    TCCCGGCCCC TCTATAGCCC CCTGCAGCCG GACATTGAGC AGGGGAAGCT GCAGATGTGG
    ATCGACCTGT TTCCAAAGGC CCTGGGGCGG CCTGGACCTC CCTTCAACAT CACTCCACGG
    AGAGCCAGAA GGTTTTTCCT GCGTTGCATT ATCTGGAACA CCAAGGATGT GATCCTGGAC
    GACCTCAGCA TCACAGGAGA GAAGATGAGC GACATTTATG TGAAAGGTTG GATGGTTGGC
    TTTGAAGAAC ACAAGCAAAA GACAGATGTG CATTATCGGT CCCTGGGAGG CGAAGGGAAC
    TTTAACTGGA GGTTCATTTT CCCCTTTGAC TACCTGCTGG CTGAGCAAGT CTGCACCGTC
    TCCAAGAAGG ATGCTTTCTG GAAGCTGGAC AAGACCGAGA GCAAAATCCC AGCACGAGTG
    GTATTCCAGA TCTGGGACAA TGACAAGTTC TCCTTTGATG ATTTCCTGGG CTCCTTGCAG
    CTGGATCTCA ACCGCATGCC CAAGCCTGCC AAGACAGCCG AGAAGTGCTC CCTGGACCAG
    CTGGATGACA CTTTCCACCC AGAGTGGTTT GTGTCCCTTT TTGAACAGAA AACTGTGAAG
    GGCTGGTGGC CCTGTGTGGC GGAAGAGGGT GAGAAGAAGA AGCTGGCGGG CAAGCTGGAG
    ATGACCTTGG AGATTGTGGC GGAGAGTGAG CATGAGGAGC GGCCCGCTGG CCAGGGCCGG
    GATGAGCCCA ACATGAACCC CAAGCTCGAG GACCCAAGGC GCCCTGACAC CTCCTTCCTC
    TGGTTCACCT CCCCGTACAA GACTATGAAG TTCATCCTGT GGCGGCGTTT CCGGTGTGCC
    ATCATCCTCT TCATCATCCT CTTCATCCTA CTGCTGTTCT TGGGCATCTT CGTTTACGCC
    TTCCCGAACT ATGCTGCCAT GAAGCTGGTG AAGCCCTTCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023474682.1
    CDS210..6554
    Translation

    Target ORF information:

    RefSeq Version XM_023618914.1
    Organism Equus caballus(horse)
    Definition Equus caballus dysferlin (DYSF), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023618914.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGTGCT GCCTGCTAGT GAAGGCCAGC AACCTTCCCA CTGTGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAA
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG GGTCCCCCTG
    GACCAGAGCT CTGAGCTTCA CGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
    TTCCTGGGAG AAGCCAAGGT CCCACTCCGG GAGGTCCTTG CCACCCCCAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACGCCTCT CCCTGGAGCT GTGCCCCTGT TCCCACCCTC TGTGCCGCTG
    GAACCCTCCC CGACTCTGCC CGATGTGGAT ATGCTGGCTG GTGGGGGACA GAGCCGGGCC
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAATGG
    CAGGCCCCCA CGGATACAGG AGGAGAGGAG GACACCGAGG ACCAGGGGCT CACGGGAGAT
    GAGGCAGAGC CATTCTTGGA TCAGAACGGA GCCCCAGGCC CCGGGGCTCC CACCACCCTG
    AAGAAGCCAC CTTCCCATCC TCCCCCCTAC CATCCTGGGG GGAAAAGGAA GAGAAGCACG
    CCTGCGCCCA GAAAGCTGCT TTCGGATAAA CCACAGGACT TCCAGATCAG GGTCCAGGTG
    ATCGAGGGGC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCGGCC
    AGGCAGACCA AGCGGACTCG GATTCACAGG GGAAACAGCC CGCTCTTCAA CGAGACTCTC
    TTCTTCAACG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGGCCGTCTT TATCACGGTG
    GTAGACTCCT GTTCGCTCCG GACAGATGCC CTCATCGGGG AGTTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCT
    GACGATTTCT CTGCTGGGCC CAAAGGCTAC CTGAAAGCAA GCCTTTGTGT GCTGGGGCCT
    GGAGACGAAG CTCCGCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAGC
    AATCTGCTCA GGCCAACTGG CATGGCCCTT CGAGGAGCGC ACTTCTGTCT GAAGGTCTTC
    AGGGCTGAGG ACTTACCACA GATGGACGAT GCCGTGGTGG ACAGCGTGAA GCAGATCTTC
    GGCTTTGACA GTAACAAGAA GAACCTGGTG GATCCCTTCG TCGAGGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ATGGCCAACC CTCAGTGGAA CCAGAGCATC
    ACGCTGCCTG TCATGTTTCC CTCCATGTCT GAAAAAATGA GGATTCGTGT CATAGACTGG
    GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAATATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGC AGAGGAGCCT GCAGGTGTGG TCAAGCCTCC TCCAGCCACG
    GACCTGGACG ACCACCTGGG CTTCCTCCCC ACCTTCGGGC CCTGCTATGT CAACCTCTAC
    GGCAGCCCCA GGGAGTTCAC TGGCTTCCCA GACCCCTATG CAGAGCTCAA CACGGGCAAG
    GGAGAAGGCG TGGCCTACCG AGGCCGGCTT CTGGTCTCCC TGGAGACCAA ACTGGTGGAG
    CAAGGCGAGC AGAAGGTGGA GGATCTCGCT GCAGATGACA TCCTTCGGGT GGAGAAGTAC
    CTCCGGAGGC GCAAGTACTC CCTCTTTGCT GCCTTCTACT CGGCCTGCAT GCTGCAGGAT
    GTCGACGATG CCATCCAGTT CGAGGTCAGC ATCGGAAACT ACGGGAACAA GTTTGACACG
    ACCTGCCTGC CGCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA CGGGTGCCAC
    TACTACTACT TACCCTGGGG CAACGTGAAG CCTGTGGTGG TGCTGTCATC CTACTGGGAA
    GACATCAGCC ACAGAGTCGA CACTCAGAAC CAGCTGCTCC GGATCGCCGA CCGGCTGGAA
    GCCGGCCTGG AGCAGGTCCA CCTGGCCCTG AAGGCGCGGT GCTCCACCGA GGATGTGGAC
    TCCCTGGTGG CTCAGCTGAT GGATGAGCTC ATTGCAGACT GCAGCCAGCC CCTGTGCAAC
    GTCCAAGAGA CGCCCTCTGC CACCCACCTG GACCAGTACC TGTACCGGCT GCGCACCCGT
    CACCTGAGCC AGATCAGTGA GGCGGCCCTG GCCTTGAAGC TCGGCCACAG TGAGCTCCCC
    GCCGCCCTTG AGCAGGCTGA GGACTGGCTC CTGCGTCTCC GTGCCCTGGC GGAGGAGCCC
    CAGAACAGCC TGCCTGACGT GGTCATCTGG ATGCTGCAGG GAGACAAGCG GGTGGCGTAC
    CAGCGGGTGC CCGCCCACGA GGTCCTCTTC TCCCGGCGGG GCGCCAACTA CTGTGGCAAG
    AATTGTGGGA AGCTGCAGAC GATCTTTCTG AAGTATCCAA TGGAAGGGGT GCCGGGAGCC
    CGGATGCCGG TGCAGATACG GGTGAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAG
    TTCAATCAGT TTGCTGAGGG CAAGCTCTCT GTCTTCGCTG AAACCTACGA GAACCAGACC
    AAGCTGGCCC TAGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAC
    GTCACAGGCA AGATCAAGCT ACCCAAGGAC AGCTTCCGCC CCTCGGCCGG CTGGGCCTGG
    GCTGGAGAGT GGTTCGTGTG TCCGGAGAAG ACCCTACTCC ACGACACCGA TGCGGGCCAC
    CTGAGCTTTG TGGAGGAGGT GTTTGAGAAC CAGACCCGGC TGCCCGGAGG CCAGTGGATC
    TACATGAGTG ACAACTACAC AGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC
    GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCC
    GTTGACGAGC AAGGCTGGGA GTACAGCATC ACCATCCCTC CGGAACGGAA GCCAAGGCAC
    TGGGTCCCTG CCGAGAAGAT GTACTACACA CACCGTCGGC GGCGCTGGGT CCGCCTGCGC
    AGGAGGGACC TCAGCCAGAT GGAGGCGCTG AAGAGGCACA GGCAGGCGGA GGCGGAGGGC
    GAGGGCTGGG AGTACGCCTC GCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACC
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CACTGGAGAA GACAGGGCCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCGTGG TGGACGACAG GAGTGAAGAT
    TCGGTGTCCG TCTCCACCTT GAGCTTCGGC GTGAACAGAC CCACGATTTC CTGCATCTTC
    GACTACGGGA ACCGCTACCA TCTACGCTGC TACATGTACC AGGCCCGGGA CCTGCCCGCC
    ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCAATCGTCT CCTTCCTGCA CCAGAGCCAG
    AAGACAGTGG TGGTGAAGAA CACCCTGAAC CCCACCTGGG ACCAGACACT CATCTTCTAT
    GAGATCGAGA TCTTTGGTGA GCCGCCCAGC ATCGCCGAGC AGCCGCCCAG CATCGTGGTG
    GAGCTATACG ACCATGACAC CTACGGTGCA GATGAGTTTA TGGGACGCTG CATCTGTCAG
    CCGACTCTGG AACGGATGCC CCGGCTGACC TGGTTCCCAC TGACTAGGGG CAGCCAGCCG
    GCGGGCGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCGGC CATCTACCAC
    ATTCCTGGTT TTGAGGTGCA GGATACATCA GGGATCCTGG AAGAGTCGGA GGACACAGAC
    CTGCCCTACC CGCCACCCCA GAGAGAGGCC AACATCTACA TGGTTCCCCA GAACATCAAG
    CCTGCTCTCC AGCGGACGGC CATCGAGATC CTGGCATGGG GCCTGCGGAA CATGAAGAGT
    TACCACCTGG CCAGTGTCTC CTCCCCCAGC CTCGTGGTGG AGTGCGGGGG CCAGACGGTG
    CAGTCCTGTG TCATCAGGAA CCTCCAGAAG AACCCCAACT TCGACGTCTG CACCCTCTTC
    ATGGAAGTGA TGCTGCCCAG GGAGGAGCTC TACTGTCCCC CCATCGTGGT CAAGGTCATC
    GATAACTGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCTG CTCCCTGGAG
    AGCTTCCTGT GTGACCCCTA CTCAGAGGAG AGTCCGTCCC CACAGGGCGG CCCAGATGAT
    GTGAGCTTAC TCAGTCCTGG GGAAGATGTG CTCATTGACA TTGATGACAA GGAGCCCCTC
    ATCCCTATGC AGGAGGAGGA GTTCATCGAT TGGTGGAGCA AATTCTTTGC CTCGATAGGG
    GAGAGGGAAA AGTGCGGGTC CTACTTGGAG AAGGACTTTG ACACTCTAAA GGTCTATGAC
    ACACAACTGG AGAATGTGGA GGCCTTTGAG GGCCTGTCTG ACTTTTGTAA CACCTTCAAG
    CTCTACCGGG GCAAGACTCA GGAGGAGATG GAAGACCCAT CTGTCGTTGG GGAATTTAAG
    GGCCTCTTCA AAATTTATCC CCTCCCAGAA GACCCGGCCA TCCCCATGCC CCCAAGACAG
    TTCCACCAGC TAGCTGCCCA GGGACCCCAG GAGTGCCTGG TCCGCATCTA CATCATCCGG
    GCATTCGGCC TGCAGCCCAA GGACCCCAAT GGGAAGTGTG ACCCTTACAT CAAGATCTCC
    ATAGGGAAGA AATCCGTGAG CGACCAGGAT AACTACATCC CCTGCACTCT GGAGCCTGTG
    TTTGGAAAGA TGTTCGAACT GACCTGCACT CTGCCTCTGG AGAAGGACCT GAAGATCACT
    CTCTATGACT ATGACCTCCT CTCCAAGGAC GAGATGATCG GGGAGACGGT CATCGACCTG
    GAGAATAGGC TGCTGTCCAA GTTTGGAGCT CGCTGCGGAC TCCCACAGAC CTACTGTGTC
    TCTGGACCGA ACCAGTGGAG GGACCAGCTC CGCCCCTCCC AGCTTCTCCA CCTGTTCTGC
    CAGCAGCAGA GAGTCAAGGC CCCCGTGTAC CAGACCGACC GTGTGGTGTT TGGGAATAAA
    GAATACACGA TTGAAGAAAT AGAGGCTGGG AGGGTCCTGA ATCCACACCT GGGCCCAGTG
    GAAGAGCGCC TGGCCTTGCA CGTCCTCCAG CAGCAGGGCT TGGTGCCCGA GCATGTGGAG
    TCCCGGCCCC TCTATAGCCC CCTGCAGCCG GACATTGAGC AGGGGAAGCT GCAGATGTGG
    ATCGACCTGT TTCCAAAGGC CCTGGGGCGG CCTGGACCTC CCTTCAACAT CACTCCACGG
    AGAGCCAGAA GGTTTTTCCT GCGTTGCATT ATCTGGAACA CCAAGGATGT GATCCTGGAC
    GACCTCAGCA TCACAGGAGA GAAGATGAGC GACATTTATG TGAAAGGTTG GATGGTTGGC
    TTTGAAGAAC ACAAGCAAAA GACAGATGTG CATTATCGGT CCCTGGGAGG CGAAGGGAAC
    TTTAACTGGA GGTTCATTTT CCCCTTTGAC TACCTGCTGG CTGAGCAAGT CTGCACCGTC
    TCCAAGAAGG ATGCTTTCTG GAAGCTGGAC AAGACCGAGA GCAAAATCCC AGCACGAGTG
    GTATTCCAGA TCTGGGACAA TGACAAGTTC TCCTTTGATG ATTTCCTGGG CTCCTTGCAG
    CTGGATCTCA ACCGCATGCC CAAGCCTGCC AAGACAGCCG AGAAGTGCTC CCTGGACCAG
    CTGGATGACA CTTTCCACCC AGAGTGGTTT GTGTCCCTTT TTGAACAGAA AACTGTGAAG
    GGCTGGTGGC CCTGTGTGGC GGAAGAGGGT GAGAAGAAGA AGCTGGCGGG CAAGCTGGAG
    ATGACCTTGG AGATTGTGGC GGAGAGTGAG CATGAGGAGC GGCCCGCTGG CCAGGGCCGG
    GATGAGCCCA ACATGAACCC CAAGCTCGAG GACCCAAGGC GCCCTGACAC CTCCTTCCTC
    TGGTTCACCT CCCCGTACAA GACTATGAAG TTCATCCTGT GGCGGCGTTT CCGGTGTGCC
    ATCATCCTCT TCATCATCCT CTTCATCCTA CTGCTGTTCT TGGGCATCTT CGTTTACGCC
    TTCCCGAACT ATGCTGCCAT GAAGCTGGTG AAGCCCTTCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb49783
    Clone ID Related Accession (Same CDS sequence) XM_023618915.1
    Accession Version XM_023618915.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6315bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product dysferlin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009158.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGTGCT GCCTGCTAGT GAAGGCCAGC AACCTTCCCA CTGTGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAA
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG GGTCCCCCTG
    GACCAGAGCT CTGAGCTTCA CGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
    TTCCTGGGAG AAGCCAAGGT CCCACTCCGG GAGGTCCTTG CCACCCCCAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACGCCTCT CCCTGGAGCT GTGCCCCTGT TCCCACCCTC TGTGCCGCTG
    GAACCCTCCC CGACTCTGCC CGATGTGGAT ATGCTGGCTG ATACAGGAGG AGAGGAGGAC
    ACCGAGGACC AGGGGCTCAC GGGAGATGAG GCAGAGCCAT TCTTGGATCA GAACGGAGCC
    CCAGGCCCCG GGGCTCCCAC CACCCTGAAG AAGCCACCTT CCCATCCTCC CCCCTACCAT
    CCTGGGGGGA AAAGGAAGAG AAGCACGCCT GCGCCCAGAA AGCTGCTTTC GGATAAACCA
    CAGGACTTCC AGATCAGGGT CCAGGTGATC GAGGGGCGCC AGCTGCCAGG GGTGAACATC
    AAGCCTGTGG TCAAGGTCAC CGCGGCCAGG CAGACCAAGC GGACTCGGAT TCACAGGGGA
    AACAGCCCGC TCTTCAACGA GACTCTCTTC TTCAACGTGT TTGACTCTCC CTCGGAGCTG
    TTTGACGAGG CCGTCTTTAT CACGGTGGTA GACTCCTGTT CGCTCCGGAC AGATGCCCTC
    ATCGGGGAGT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA CGCCTATCTC
    AGGAAGTGGC TGCTGCTCTC GGACCCTGAC GATTTCTCTG CTGGGCCCAA AGGCTACCTG
    AAAGCAAGCC TTTGTGTGCT GGGGCCTGGA GACGAAGCTC CGCTGGAGAG AAAGGACCCC
    TCTGAAGACA AGGAGGACAT TGAAAGCAAT CTGCTCAGGC CAACTGGCAT GGCCCTTCGA
    GGAGCGCACT TCTGTCTGAA GGTCTTCAGG GCTGAGGACT TACCACAGAT GGACGATGCC
    GTGGTGGACA GCGTGAAGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CCTGGTGGAT
    CCCTTCGTCG AGGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGATG
    GCCAACCCTC AGTGGAACCA GAGCATCACG CTGCCTGTCA TGTTTCCCTC CATGTCTGAA
    AAAATGAGGA TTCGTGTCAT AGACTGGGAC CGCCTCACCC ACAATGACAT CGTGGCCACC
    ACCTACCTGA ATATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGCAGA GGAGCCTGCA
    GGTGTGGTCA AGCCTCCTCC AGCCACGGAC CTGGACGACC ACCTGGGCTT CCTCCCCACC
    TTCGGGCCCT GCTATGTCAA CCTCTACGGC AGCCCCAGGG AGTTCACTGG CTTCCCAGAC
    CCCTATGCAG AGCTCAACAC GGGCAAGGGA GAAGGCGTGG CCTACCGAGG CCGGCTTCTG
    GTCTCCCTGG AGACCAAACT GGTGGAGCAA GGCGAGCAGA AGGTGGAGGA TCTCGCTGCA
    GATGACATCC TTCGGGTGGA GAAGTACCTC CGGAGGCGCA AGTACTCCCT CTTTGCTGCC
    TTCTACTCGG CCTGCATGCT GCAGGATGTC GACGATGCCA TCCAGTTCGA GGTCAGCATC
    GGAAACTACG GGAACAAGTT TGACACGACC TGCCTGCCGC TGGCCTCCAC CACCCAGTAC
    AGCCGGGCGG TCTTTGACGG GTGCCACTAC TACTACTTAC CCTGGGGCAA CGTGAAGCCT
    GTGGTGGTGC TGTCATCCTA CTGGGAAGAC ATCAGCCACA GAGTCGACAC TCAGAACCAG
    CTGCTCCGGA TCGCCGACCG GCTGGAAGCC GGCCTGGAGC AGGTCCACCT GGCCCTGAAG
    GCGCGGTGCT CCACCGAGGA TGTGGACTCC CTGGTGGCTC AGCTGATGGA TGAGCTCATT
    GCAGACTGCA GCCAGCCCCT GTGCAACGTC CAAGAGACGC CCTCTGCCAC CCACCTGGAC
    CAGTACCTGT ACCGGCTGCG CACCCGTCAC CTGAGCCAGA TCAGTGAGGC GGCCCTGGCC
    TTGAAGCTCG GCCACAGTGA GCTCCCCGCC GCCCTTGAGC AGGCTGAGGA CTGGCTCCTG
    CGTCTCCGTG CCCTGGCGGA GGAGCCCCAG AACAGCCTGC CTGACGTGGT CATCTGGATG
    CTGCAGGGAG ACAAGCGGGT GGCGTACCAG CGGGTGCCCG CCCACGAGGT CCTCTTCTCC
    CGGCGGGGCG CCAACTACTG TGGCAAGAAT TGTGGGAAGC TGCAGACGAT CTTTCTGAAG
    TATCCAATGG AAGGGGTGCC GGGAGCCCGG ATGCCGGTGC AGATACGGGT GAAGCTCTGG
    TTCGGGCTCT CTGTGGATGA GAAGGAGTTC AATCAGTTTG CTGAGGGCAA GCTCTCTGTC
    TTCGCTGAAA CCTACGAGAA CCAGACCAAG CTGGCCCTAG TCGGGAACTG GGGCACAACG
    GGCCTCACCT ACCCCAAGTT TTCTGACGTC ACAGGCAAGA TCAAGCTACC CAAGGACAGC
    TTCCGCCCCT CGGCCGGCTG GGCCTGGGCT GGAGAGTGGT TCGTGTGTCC GGAGAAGACC
    CTACTCCACG ACACCGATGC GGGCCACCTG AGCTTTGTGG AGGAGGTGTT TGAGAACCAG
    ACCCGGCTGC CCGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACAGA TGTGAACGGG
    GAGAAGGTGC TTCCCAAGGA TGACATCGAG TGCCCACTGG GCTGGAAGTG GGAAGATGAA
    GAATGGTCCA CAGACCTCAA TCGGGCCGTT GACGAGCAAG GCTGGGAGTA CAGCATCACC
    ATCCCTCCGG AACGGAAGCC AAGGCACTGG GTCCCTGCCG AGAAGATGTA CTACACACAC
    CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG AGGGACCTCA GCCAGATGGA GGCGCTGAAG
    AGGCACAGGC AGGCGGAGGC GGAGGGCGAG GGCTGGGAGT ACGCCTCGCT CTTCGGCTGG
    AAGTTCCACC TGGAGTACCG CAAGACCGAT GCCTTCCGCC GCCGCCGCTG GCGCCGCCGC
    ATGGAGCCAC TGGAGAAGAC AGGGCCTGCA GCTGTGTTTG CCCTCGAGGG GGCCCTGGGC
    GGCGTGGTGG ACGACAGGAG TGAAGATTCG GTGTCCGTCT CCACCTTGAG CTTCGGCGTG
    AACAGACCCA CGATTTCCTG CATCTTCGAC TACGGGAACC GCTACCATCT ACGCTGCTAC
    ATGTACCAGG CCCGGGACCT GCCCGCCATG GACAAGGACT CTTTTTCTGA TCCCTACGCA
    ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG TGAAGAACAC CCTGAACCCC
    ACCTGGGACC AGACACTCAT CTTCTATGAG ATCGAGATCT TTGGTGAGCC GCCCAGCATC
    GCCGAGCAGC CGCCCAGCAT CGTGGTGGAG CTATACGACC ATGACACCTA CGGTGCAGAT
    GAGTTTATGG GACGCTGCAT CTGTCAGCCG ACTCTGGAAC GGATGCCCCG GCTGACCTGG
    TTCCCACTGA CTAGGGGCAG CCAGCCGGCG GGCGAGCTGC TGGCCTCTTT TGAGCTCATC
    CAGAGAGAGA AGCCGGCCAT CTACCACATT CCTGGTTTTG AGGTGCAGGA TACATCAGGG
    ATCCTGGAAG AGTCGGAGGA CACAGACCTG CCCTACCCGC CACCCCAGAG AGAGGCCAAC
    ATCTACATGG TTCCCCAGAA CATCAAGCCT GCTCTCCAGC GGACGGCCAT CGAGATCCTG
    GCATGGGGCC TGCGGAACAT GAAGAGTTAC CACCTGGCCA GTGTCTCCTC CCCCAGCCTC
    GTGGTGGAGT GCGGGGGCCA GACGGTGCAG TCCTGTGTCA TCAGGAACCT CCAGAAGAAC
    CCCAACTTCG ACGTCTGCAC CCTCTTCATG GAAGTGATGC TGCCCAGGGA GGAGCTCTAC
    TGTCCCCCCA TCGTGGTCAA GGTCATCGAT AACTGCCAGT TTGGCCGCCG GCCTGTGGTG
    GGCCAGTGTA CCATCTGCTC CCTGGAGAGC TTCCTGTGTG ACCCCTACTC AGAGGAGAGT
    CCGTCCCCAC AGGGCGGCCC AGATGATGTG AGCTTACTCA GTCCTGGGGA AGATGTGCTC
    ATTGACATTG ATGACAAGGA GCCCCTCATC CCTATGCAGC TTGCAGATGG TCTGTCGGGC
    TCGGCCCCCA CTAACATGGC ATCTTCTCCG TCCAGTCCTC ATGAGGAGGA GTTCATCGAT
    TGGTGGAGCA AATTCTTTGC CTCGATAGGG GAGAGGGAAA AGTGCGGGTC CTACTTGGAG
    AAGGACTTTG ACACTCTAAA GGTCTATGAC ACACAACTGG AGAATGTGGA GGCCTTTGAG
    GGCCTGTCTG ACTTTTGTAA CACCTTCAAG CTCTACCGGG GCAAGACTCA GGAGGAGATG
    GAAGACCCAT CTGTCGTTGG GGAATTTAAG GGCCTCTTCA AAATTTATCC CCTCCCAGAA
    GACCCGGCCA TCCCCATGCC CCCAAGACAG TTCCACCAGC TAGCTGCCCA GGGACCCCAG
    GAGTGCCTGG TCCGCATCTA CATCATCCGG GCATTCGGCC TGCAGCCCAA GGACCCCAAT
    GGGAAGTGTG ACCCTTACAT CAAGATCTCC ATAGGGAAGA AATCCGTGAG CGACCAGGAT
    AACTACATCC CCTGCACTCT GGAGCCTGTG TTTGGAAAGA TGTTCGAACT GACCTGCACT
    CTGCCTCTGG AGAAGGACCT GAAGATCACT CTCTATGACT ATGACCTCCT CTCCAAGGAC
    GAGATGATCG GGGAGACGGT CATCGACCTG GAGAATAGGC TGCTGTCCAA GTTTGGAGCT
    CGCTGCGGAC TCCCACAGAC CTACTGTGTC TCTGGACCGA ACCAGTGGAG GGACCAGCTC
    CGCCCCTCCC AGCTTCTCCA CCTGTTCTGC CAGCAGCAGA GAGTCAAGGC CCCCGTGTAC
    CAGACCGACC GTGTGGTGTT TGGGAATAAA GAATACACGA TTGAAGAAAT AGAGGCTGGG
    AGGGTCCTGA ATCCACACCT GGGCCCAGTG GAAGAGCGCC TGGCCTTGCA CGTCCTCCAG
    CAGCAGGGCT TGGTGCCCGA GCATGTGGAG TCCCGGCCCC TCTATAGCCC CCTGCAGCCG
    GACATTGAGC AGGGGAAGCT GCAGATGTGG ATCGACCTGT TTCCAAAGGC CCTGGGGCGG
    CCTGGACCTC CCTTCAACAT CACTCCACGG AGAGCCAGAA GGTTTTTCCT GCGTTGCATT
    ATCTGGAACA CCAAGGATGT GATCCTGGAC GACCTCAGCA TCACAGGAGA GAAGATGAGC
    GACATTTATG TGAAAGGTTG GATGGTTGGC TTTGAAGAAC ACAAGCAAAA GACAGATGTG
    CATTATCGGT CCCTGGGAGG CGAAGGGAAC TTTAACTGGA GGTTCATTTT CCCCTTTGAC
    TACCTGCTGG CTGAGCAAGT CTGCACCGTC TCCAAGAAGG ATGCTTTCTG GAAGCTGGAC
    AAGACCGAGA GCAAAATCCC AGCACGAGTG GTATTCCAGA TCTGGGACAA TGACAAGTTC
    TCCTTTGATG ATTTCCTGGG CTCCTTGCAG CTGGATCTCA ACCGCATGCC CAAGCCTGCC
    AAGACAGCCG AGAAGTGCTC CCTGGACCAG CTGGATGACA CTTTCCACCC AGAGTGGTTT
    GTGTCCCTTT TTGAACAGAA AACTGTGAAG GGCTGGTGGC CCTGTGTGGC GGAAGAGGGT
    GAGAAGAAGA AGCTGGCGGG CAAGCTGGAG ATGACCTTGG AGATTGTGGC GGAGAGTGAG
    CATGAGGAGC GGCCCGCTGG CCAGGGCCGG GATGAGCCCA ACATGAACCC CAAGCTCGAG
    GACCCAAGGC GCCCTGACAC CTCCTTCCTC TGGTTCACCT CCCCGTACAA GACTATGAAG
    TTCATCCTGT GGCGGCGTTT CCGGTGTGCC ATCATCCTCT TCATCATCCT CTTCATCCTA
    CTGCTGTTCT TGGGCATCTT CGTTTACGCC TTCCCGAACT ATGCTGCCAT GAAGCTGGTG
    AAGCCCTTCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023474683.1
    CDS210..6524
    Translation

    Target ORF information:

    RefSeq Version XM_023618915.1
    Organism Equus caballus(horse)
    Definition Equus caballus dysferlin (DYSF), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023618915.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGTGCT GCCTGCTAGT GAAGGCCAGC AACCTTCCCA CTGTGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAA
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG GGTCCCCCTG
    GACCAGAGCT CTGAGCTTCA CGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
    TTCCTGGGAG AAGCCAAGGT CCCACTCCGG GAGGTCCTTG CCACCCCCAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACGCCTCT CCCTGGAGCT GTGCCCCTGT TCCCACCCTC TGTGCCGCTG
    GAACCCTCCC CGACTCTGCC CGATGTGGAT ATGCTGGCTG ATACAGGAGG AGAGGAGGAC
    ACCGAGGACC AGGGGCTCAC GGGAGATGAG GCAGAGCCAT TCTTGGATCA GAACGGAGCC
    CCAGGCCCCG GGGCTCCCAC CACCCTGAAG AAGCCACCTT CCCATCCTCC CCCCTACCAT
    CCTGGGGGGA AAAGGAAGAG AAGCACGCCT GCGCCCAGAA AGCTGCTTTC GGATAAACCA
    CAGGACTTCC AGATCAGGGT CCAGGTGATC GAGGGGCGCC AGCTGCCAGG GGTGAACATC
    AAGCCTGTGG TCAAGGTCAC CGCGGCCAGG CAGACCAAGC GGACTCGGAT TCACAGGGGA
    AACAGCCCGC TCTTCAACGA GACTCTCTTC TTCAACGTGT TTGACTCTCC CTCGGAGCTG
    TTTGACGAGG CCGTCTTTAT CACGGTGGTA GACTCCTGTT CGCTCCGGAC AGATGCCCTC
    ATCGGGGAGT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA CGCCTATCTC
    AGGAAGTGGC TGCTGCTCTC GGACCCTGAC GATTTCTCTG CTGGGCCCAA AGGCTACCTG
    AAAGCAAGCC TTTGTGTGCT GGGGCCTGGA GACGAAGCTC CGCTGGAGAG AAAGGACCCC
    TCTGAAGACA AGGAGGACAT TGAAAGCAAT CTGCTCAGGC CAACTGGCAT GGCCCTTCGA
    GGAGCGCACT TCTGTCTGAA GGTCTTCAGG GCTGAGGACT TACCACAGAT GGACGATGCC
    GTGGTGGACA GCGTGAAGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CCTGGTGGAT
    CCCTTCGTCG AGGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGATG
    GCCAACCCTC AGTGGAACCA GAGCATCACG CTGCCTGTCA TGTTTCCCTC CATGTCTGAA
    AAAATGAGGA TTCGTGTCAT AGACTGGGAC CGCCTCACCC ACAATGACAT CGTGGCCACC
    ACCTACCTGA ATATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGCAGA GGAGCCTGCA
    GGTGTGGTCA AGCCTCCTCC AGCCACGGAC CTGGACGACC ACCTGGGCTT CCTCCCCACC
    TTCGGGCCCT GCTATGTCAA CCTCTACGGC AGCCCCAGGG AGTTCACTGG CTTCCCAGAC
    CCCTATGCAG AGCTCAACAC GGGCAAGGGA GAAGGCGTGG CCTACCGAGG CCGGCTTCTG
    GTCTCCCTGG AGACCAAACT GGTGGAGCAA GGCGAGCAGA AGGTGGAGGA TCTCGCTGCA
    GATGACATCC TTCGGGTGGA GAAGTACCTC CGGAGGCGCA AGTACTCCCT CTTTGCTGCC
    TTCTACTCGG CCTGCATGCT GCAGGATGTC GACGATGCCA TCCAGTTCGA GGTCAGCATC
    GGAAACTACG GGAACAAGTT TGACACGACC TGCCTGCCGC TGGCCTCCAC CACCCAGTAC
    AGCCGGGCGG TCTTTGACGG GTGCCACTAC TACTACTTAC CCTGGGGCAA CGTGAAGCCT
    GTGGTGGTGC TGTCATCCTA CTGGGAAGAC ATCAGCCACA GAGTCGACAC TCAGAACCAG
    CTGCTCCGGA TCGCCGACCG GCTGGAAGCC GGCCTGGAGC AGGTCCACCT GGCCCTGAAG
    GCGCGGTGCT CCACCGAGGA TGTGGACTCC CTGGTGGCTC AGCTGATGGA TGAGCTCATT
    GCAGACTGCA GCCAGCCCCT GTGCAACGTC CAAGAGACGC CCTCTGCCAC CCACCTGGAC
    CAGTACCTGT ACCGGCTGCG CACCCGTCAC CTGAGCCAGA TCAGTGAGGC GGCCCTGGCC
    TTGAAGCTCG GCCACAGTGA GCTCCCCGCC GCCCTTGAGC AGGCTGAGGA CTGGCTCCTG
    CGTCTCCGTG CCCTGGCGGA GGAGCCCCAG AACAGCCTGC CTGACGTGGT CATCTGGATG
    CTGCAGGGAG ACAAGCGGGT GGCGTACCAG CGGGTGCCCG CCCACGAGGT CCTCTTCTCC
    CGGCGGGGCG CCAACTACTG TGGCAAGAAT TGTGGGAAGC TGCAGACGAT CTTTCTGAAG
    TATCCAATGG AAGGGGTGCC GGGAGCCCGG ATGCCGGTGC AGATACGGGT GAAGCTCTGG
    TTCGGGCTCT CTGTGGATGA GAAGGAGTTC AATCAGTTTG CTGAGGGCAA GCTCTCTGTC
    TTCGCTGAAA CCTACGAGAA CCAGACCAAG CTGGCCCTAG TCGGGAACTG GGGCACAACG
    GGCCTCACCT ACCCCAAGTT TTCTGACGTC ACAGGCAAGA TCAAGCTACC CAAGGACAGC
    TTCCGCCCCT CGGCCGGCTG GGCCTGGGCT GGAGAGTGGT TCGTGTGTCC GGAGAAGACC
    CTACTCCACG ACACCGATGC GGGCCACCTG AGCTTTGTGG AGGAGGTGTT TGAGAACCAG
    ACCCGGCTGC CCGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACAGA TGTGAACGGG
    GAGAAGGTGC TTCCCAAGGA TGACATCGAG TGCCCACTGG GCTGGAAGTG GGAAGATGAA
    GAATGGTCCA CAGACCTCAA TCGGGCCGTT GACGAGCAAG GCTGGGAGTA CAGCATCACC
    ATCCCTCCGG AACGGAAGCC AAGGCACTGG GTCCCTGCCG AGAAGATGTA CTACACACAC
    CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG AGGGACCTCA GCCAGATGGA GGCGCTGAAG
    AGGCACAGGC AGGCGGAGGC GGAGGGCGAG GGCTGGGAGT ACGCCTCGCT CTTCGGCTGG
    AAGTTCCACC TGGAGTACCG CAAGACCGAT GCCTTCCGCC GCCGCCGCTG GCGCCGCCGC
    ATGGAGCCAC TGGAGAAGAC AGGGCCTGCA GCTGTGTTTG CCCTCGAGGG GGCCCTGGGC
    GGCGTGGTGG ACGACAGGAG TGAAGATTCG GTGTCCGTCT CCACCTTGAG CTTCGGCGTG
    AACAGACCCA CGATTTCCTG CATCTTCGAC TACGGGAACC GCTACCATCT ACGCTGCTAC
    ATGTACCAGG CCCGGGACCT GCCCGCCATG GACAAGGACT CTTTTTCTGA TCCCTACGCA
    ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG TGAAGAACAC CCTGAACCCC
    ACCTGGGACC AGACACTCAT CTTCTATGAG ATCGAGATCT TTGGTGAGCC GCCCAGCATC
    GCCGAGCAGC CGCCCAGCAT CGTGGTGGAG CTATACGACC ATGACACCTA CGGTGCAGAT
    GAGTTTATGG GACGCTGCAT CTGTCAGCCG ACTCTGGAAC GGATGCCCCG GCTGACCTGG
    TTCCCACTGA CTAGGGGCAG CCAGCCGGCG GGCGAGCTGC TGGCCTCTTT TGAGCTCATC
    CAGAGAGAGA AGCCGGCCAT CTACCACATT CCTGGTTTTG AGGTGCAGGA TACATCAGGG
    ATCCTGGAAG AGTCGGAGGA CACAGACCTG CCCTACCCGC CACCCCAGAG AGAGGCCAAC
    ATCTACATGG TTCCCCAGAA CATCAAGCCT GCTCTCCAGC GGACGGCCAT CGAGATCCTG
    GCATGGGGCC TGCGGAACAT GAAGAGTTAC CACCTGGCCA GTGTCTCCTC CCCCAGCCTC
    GTGGTGGAGT GCGGGGGCCA GACGGTGCAG TCCTGTGTCA TCAGGAACCT CCAGAAGAAC
    CCCAACTTCG ACGTCTGCAC CCTCTTCATG GAAGTGATGC TGCCCAGGGA GGAGCTCTAC
    TGTCCCCCCA TCGTGGTCAA GGTCATCGAT AACTGCCAGT TTGGCCGCCG GCCTGTGGTG
    GGCCAGTGTA CCATCTGCTC CCTGGAGAGC TTCCTGTGTG ACCCCTACTC AGAGGAGAGT
    CCGTCCCCAC AGGGCGGCCC AGATGATGTG AGCTTACTCA GTCCTGGGGA AGATGTGCTC
    ATTGACATTG ATGACAAGGA GCCCCTCATC CCTATGCAGC TTGCAGATGG TCTGTCGGGC
    TCGGCCCCCA CTAACATGGC ATCTTCTCCG TCCAGTCCTC ATGAGGAGGA GTTCATCGAT
    TGGTGGAGCA AATTCTTTGC CTCGATAGGG GAGAGGGAAA AGTGCGGGTC CTACTTGGAG
    AAGGACTTTG ACACTCTAAA GGTCTATGAC ACACAACTGG AGAATGTGGA GGCCTTTGAG
    GGCCTGTCTG ACTTTTGTAA CACCTTCAAG CTCTACCGGG GCAAGACTCA GGAGGAGATG
    GAAGACCCAT CTGTCGTTGG GGAATTTAAG GGCCTCTTCA AAATTTATCC CCTCCCAGAA
    GACCCGGCCA TCCCCATGCC CCCAAGACAG TTCCACCAGC TAGCTGCCCA GGGACCCCAG
    GAGTGCCTGG TCCGCATCTA CATCATCCGG GCATTCGGCC TGCAGCCCAA GGACCCCAAT
    GGGAAGTGTG ACCCTTACAT CAAGATCTCC ATAGGGAAGA AATCCGTGAG CGACCAGGAT
    AACTACATCC CCTGCACTCT GGAGCCTGTG TTTGGAAAGA TGTTCGAACT GACCTGCACT
    CTGCCTCTGG AGAAGGACCT GAAGATCACT CTCTATGACT ATGACCTCCT CTCCAAGGAC
    GAGATGATCG GGGAGACGGT CATCGACCTG GAGAATAGGC TGCTGTCCAA GTTTGGAGCT
    CGCTGCGGAC TCCCACAGAC CTACTGTGTC TCTGGACCGA ACCAGTGGAG GGACCAGCTC
    CGCCCCTCCC AGCTTCTCCA CCTGTTCTGC CAGCAGCAGA GAGTCAAGGC CCCCGTGTAC
    CAGACCGACC GTGTGGTGTT TGGGAATAAA GAATACACGA TTGAAGAAAT AGAGGCTGGG
    AGGGTCCTGA ATCCACACCT GGGCCCAGTG GAAGAGCGCC TGGCCTTGCA CGTCCTCCAG
    CAGCAGGGCT TGGTGCCCGA GCATGTGGAG TCCCGGCCCC TCTATAGCCC CCTGCAGCCG
    GACATTGAGC AGGGGAAGCT GCAGATGTGG ATCGACCTGT TTCCAAAGGC CCTGGGGCGG
    CCTGGACCTC CCTTCAACAT CACTCCACGG AGAGCCAGAA GGTTTTTCCT GCGTTGCATT
    ATCTGGAACA CCAAGGATGT GATCCTGGAC GACCTCAGCA TCACAGGAGA GAAGATGAGC
    GACATTTATG TGAAAGGTTG GATGGTTGGC TTTGAAGAAC ACAAGCAAAA GACAGATGTG
    CATTATCGGT CCCTGGGAGG CGAAGGGAAC TTTAACTGGA GGTTCATTTT CCCCTTTGAC
    TACCTGCTGG CTGAGCAAGT CTGCACCGTC TCCAAGAAGG ATGCTTTCTG GAAGCTGGAC
    AAGACCGAGA GCAAAATCCC AGCACGAGTG GTATTCCAGA TCTGGGACAA TGACAAGTTC
    TCCTTTGATG ATTTCCTGGG CTCCTTGCAG CTGGATCTCA ACCGCATGCC CAAGCCTGCC
    AAGACAGCCG AGAAGTGCTC CCTGGACCAG CTGGATGACA CTTTCCACCC AGAGTGGTTT
    GTGTCCCTTT TTGAACAGAA AACTGTGAAG GGCTGGTGGC CCTGTGTGGC GGAAGAGGGT
    GAGAAGAAGA AGCTGGCGGG CAAGCTGGAG ATGACCTTGG AGATTGTGGC GGAGAGTGAG
    CATGAGGAGC GGCCCGCTGG CCAGGGCCGG GATGAGCCCA ACATGAACCC CAAGCTCGAG
    GACCCAAGGC GCCCTGACAC CTCCTTCCTC TGGTTCACCT CCCCGTACAA GACTATGAAG
    TTCATCCTGT GGCGGCGTTT CCGGTGTGCC ATCATCCTCT TCATCATCCT CTTCATCCTA
    CTGCTGTTCT TGGGCATCTT CGTTTACGCC TTCCCGAACT ATGCTGCCAT GAAGCTGGTG
    AAGCCCTTCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb49784
    Clone ID Related Accession (Same CDS sequence) XM_023618916.1
    Accession Version XM_023618916.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6303bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product dysferlin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009158.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGTGCT GCCTGCTAGT GAAGGCCAGC AACCTTCCCA CTGTGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAA
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG GGTCCCCCTG
    GACCAGAGCT CTGAGCTTCA CGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
    TTCCTGGGAG AAGCCAAGGT CCCACTCCGG GAGGTCCTTG CCACCCCCAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACGCCTCT CCCTGGAGCT GTGCCCCTGT TCCCACCCTC TGTGCCGCTG
    GAACCCTCCC CGACTCTGCC CGATGTGGAT ATGCTGGCTG GTGGGGGACA GAGCCGGGCC
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAATGG
    CAGGCCCCCA CGGATACAGG AGGAGAGGAG GACACCGAGG ACCAGGGGCT CACGGGAGAT
    GAGGCAGAGC CATTCTTGGA TCAGAACGGA GCCCCAGGCC CCGGGGCTCC CACCACCCTG
    AAGAAGCCAC CTTCCCATCC TCCCCCCTAC CATCCTGGGG GGAAAAGGAA GAGAAGCACG
    CCTGCGCCCA GAAAGCTGCT TTCGGATAAA CCACAGGACT TCCAGATCAG GGTCCAGGTG
    ATCGAGGGGC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCGGCC
    AGGCAGACCA AGCGGACTCG GATTCACAGG GGAAACAGCC CGCTCTTCAA CGAGACTCTC
    TTCTTCAACG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGGCCGTCTT TATCACGGTG
    GTAGACTCCT GTTCGCTCCG GACAGATGCC CTCATCGGGG AGTTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCT
    GACGATTTCT CTGCTGGGCC CAAAGGCTAC CTGAAAGCAA GCCTTTGTGT GCTGGGGCCT
    GGAGACGAAG CTCCGCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAGC
    AATCTGCTCA GGCCAACTGG CATGGCCCTT CGAGGAGCGC ACTTCTGTCT GAAGGTCTTC
    AGGGCTGAGG ACTTACCACA GATGGACGAT GCCGTGGTGG ACAGCGTGAA GCAGATCTTC
    GGCTTTGACA GTAACAAGAA GAACCTGGTG GATCCCTTCG TCGAGGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ATGGCCAACC CTCAGTGGAA CCAGAGCATC
    ACGCTGCCTG TCATGTTTCC CTCCATGTCT GAAAAAATGA GGATTCGTGT CATAGACTGG
    GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAATATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGC AGTGGACGAC CACCTGGGCT TCCTCCCCAC CTTCGGGCCC
    TGCTATGTCA ACCTCTACGG CAGCCCCAGG GAGTTCACTG GCTTCCCAGA CCCCTATGCA
    GAGCTCAACA CGGGCAAGGG AGAAGGCGTG GCCTACCGAG GCCGGCTTCT GGTCTCCCTG
    GAGACCAAAC TGGTGGAGCA AGGCGAGCAG AAGGTGGAGG ATCTCGCTGC AGATGACATC
    CTTCGGGTGG AGAAGTACCT CCGGAGGCGC AAGTACTCCC TCTTTGCTGC CTTCTACTCG
    GCCTGCATGC TGCAGGATGT CGACGATGCC ATCCAGTTCG AGGTCAGCAT CGGAAACTAC
    GGGAACAAGT TTGACACGAC CTGCCTGCCG CTGGCCTCCA CCACCCAGTA CAGCCGGGCG
    GTCTTTGACG GGTGCCACTA CTACTACTTA CCCTGGGGCA ACGTGAAGCC TGTGGTGGTG
    CTGTCATCCT ACTGGGAAGA CATCAGCCAC AGAGTCGACA CTCAGAACCA GCTGCTCCGG
    ATCGCCGACC GGCTGGAAGC CGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCGGTGC
    TCCACCGAGG ATGTGGACTC CCTGGTGGCT CAGCTGATGG ATGAGCTCAT TGCAGACTGC
    AGCCAGCCCC TGTGCAACGT CCAAGAGACG CCCTCTGCCA CCCACCTGGA CCAGTACCTG
    TACCGGCTGC GCACCCGTCA CCTGAGCCAG ATCAGTGAGG CGGCCCTGGC CTTGAAGCTC
    GGCCACAGTG AGCTCCCCGC CGCCCTTGAG CAGGCTGAGG ACTGGCTCCT GCGTCTCCGT
    GCCCTGGCGG AGGAGCCCCA GAACAGCCTG CCTGACGTGG TCATCTGGAT GCTGCAGGGA
    GACAAGCGGG TGGCGTACCA GCGGGTGCCC GCCCACGAGG TCCTCTTCTC CCGGCGGGGC
    GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTGCAGACGA TCTTTCTGAA GTATCCAATG
    GAAGGGGTGC CGGGAGCCCG GATGCCGGTG CAGATACGGG TGAAGCTCTG GTTCGGGCTC
    TCTGTGGATG AGAAGGAGTT CAATCAGTTT GCTGAGGGCA AGCTCTCTGT CTTCGCTGAA
    ACCTACGAGA ACCAGACCAA GCTGGCCCTA GTCGGGAACT GGGGCACAAC GGGCCTCACC
    TACCCCAAGT TTTCTGACGT CACAGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC
    TCGGCCGGCT GGGCCTGGGC TGGAGAGTGG TTCGTGTGTC CGGAGAAGAC CCTACTCCAC
    GACACCGATG CGGGCCACCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTG
    CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACAG ATGTGAACGG GGAGAAGGTG
    CTTCCCAAGG ATGACATCGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA AGAATGGTCC
    ACAGACCTCA ATCGGGCCGT TGACGAGCAA GGCTGGGAGT ACAGCATCAC CATCCCTCCG
    GAACGGAAGC CAAGGCACTG GGTCCCTGCC GAGAAGATGT ACTACACACA CCGTCGGCGG
    CGCTGGGTCC GCCTGCGCAG GAGGGACCTC AGCCAGATGG AGGCGCTGAA GAGGCACAGG
    CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCGC TCTTCGGCTG GAAGTTCCAC
    CTGGAGTACC GCAAGACCGA TGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCA
    CTGGAGAAGA CAGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCCCTGGG CGGCGTGGTG
    GACGACAGGA GTGAAGATTC GGTGTCCGTC TCCACCTTGA GCTTCGGCGT GAACAGACCC
    ACGATTTCCT GCATCTTCGA CTACGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG
    GCCCGGGACC TGCCCGCCAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC AATCGTCTCC
    TTCCTGCACC AGAGCCAGAA GACAGTGGTG GTGAAGAACA CCCTGAACCC CACCTGGGAC
    CAGACACTCA TCTTCTATGA GATCGAGATC TTTGGTGAGC CGCCCAGCAT CGCCGAGCAG
    CCGCCCAGCA TCGTGGTGGA GCTATACGAC CATGACACCT ACGGTGCAGA TGAGTTTATG
    GGACGCTGCA TCTGTCAGCC GACTCTGGAA CGGATGCCCC GGCTGACCTG GTTCCCACTG
    ACTAGGGGCA GCCAGCCGGC GGGCGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG
    AAGCCGGCCA TCTACCACAT TCCTGGTTTT GAGGTGCAGG ATACATCAGG GATCCTGGAA
    GAGTCGGAGG ACACAGACCT GCCCTACCCG CCACCCCAGA GAGAGGCCAA CATCTACATG
    GTTCCCCAGA ACATCAAGCC TGCTCTCCAG CGGACGGCCA TCGAGATCCT GGCATGGGGC
    CTGCGGAACA TGAAGAGTTA CCACCTGGCC AGTGTCTCCT CCCCCAGCCT CGTGGTGGAG
    TGCGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCAGAAGAA CCCCAACTTC
    GACGTCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGTCCCCCC
    ATCGTGGTCA AGGTCATCGA TAACTGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT
    ACCATCTGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CAGAGGAGAG TCCGTCCCCA
    CAGGGCGGCC CAGATGATGT GAGCTTACTC AGTCCTGGGG AAGATGTGCT CATTGACATT
    GATGACAAGG AGCCCCTCAT CCCTATGCAG GAGGAGGAGT TCATCGATTG GTGGAGCAAA
    TTCTTTGCCT CGATAGGGGA GAGGGAAAAG TGCGGGTCCT ACTTGGAGAA GGACTTTGAC
    ACTCTAAAGG TCTATGACAC ACAACTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC
    TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGATGGA AGACCCATCT
    GTCGTTGGGG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCGGCCATC
    CCCATGCCCC CAAGACAGTT CCACCAGCTA GCTGCCCAGG GACCCCAGGA GTGCCTGGTC
    CGCATCTACA TCATCCGGGC ATTCGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAC
    CCTTACATCA AGATCTCCAT AGGGAAGAAA TCCGTGAGCG ACCAGGATAA CTACATCCCC
    TGCACTCTGG AGCCTGTGTT TGGAAAGATG TTCGAACTGA CCTGCACTCT GCCTCTGGAG
    AAGGACCTGA AGATCACTCT CTATGACTAT GACCTCCTCT CCAAGGACGA GATGATCGGG
    GAGACGGTCA TCGACCTGGA GAATAGGCTG CTGTCCAAGT TTGGAGCTCG CTGCGGACTC
    CCACAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG
    CTTCTCCACC TGTTCTGCCA GCAGCAGAGA GTCAAGGCCC CCGTGTACCA GACCGACCGT
    GTGGTGTTTG GGAATAAAGA ATACACGATT GAAGAAATAG AGGCTGGGAG GGTCCTGAAT
    CCACACCTGG GCCCAGTGGA AGAGCGCCTG GCCTTGCACG TCCTCCAGCA GCAGGGCTTG
    GTGCCCGAGC ATGTGGAGTC CCGGCCCCTC TATAGCCCCC TGCAGCCGGA CATTGAGCAG
    GGGAAGCTGC AGATGTGGAT CGACCTGTTT CCAAAGGCCC TGGGGCGGCC TGGACCTCCC
    TTCAACATCA CTCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGCATTAT CTGGAACACC
    AAGGATGTGA TCCTGGACGA CCTCAGCATC ACAGGAGAGA AGATGAGCGA CATTTATGTG
    AAAGGTTGGA TGGTTGGCTT TGAAGAACAC AAGCAAAAGA CAGATGTGCA TTATCGGTCC
    CTGGGAGGCG AAGGGAACTT TAACTGGAGG TTCATTTTCC CCTTTGACTA CCTGCTGGCT
    GAGCAAGTCT GCACCGTCTC CAAGAAGGAT GCTTTCTGGA AGCTGGACAA GACCGAGAGC
    AAAATCCCAG CACGAGTGGT ATTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT
    TTCCTGGGCT CCTTGCAGCT GGATCTCAAC CGCATGCCCA AGCCTGCCAA GACAGCCGAG
    AAGTGCTCCC TGGACCAGCT GGATGACACT TTCCACCCAG AGTGGTTTGT GTCCCTTTTT
    GAACAGAAAA CTGTGAAGGG CTGGTGGCCC TGTGTGGCGG AAGAGGGTGA GAAGAAGAAG
    CTGGCGGGCA AGCTGGAGAT GACCTTGGAG ATTGTGGCGG AGAGTGAGCA TGAGGAGCGG
    CCCGCTGGCC AGGGCCGGGA TGAGCCCAAC ATGAACCCCA AGCTCGAGGA CCCAAGGCGC
    CCTGACACCT CCTTCCTCTG GTTCACCTCC CCGTACAAGA CTATGAAGTT CATCCTGTGG
    CGGCGTTTCC GGTGTGCCAT CATCCTCTTC ATCATCCTCT TCATCCTACT GCTGTTCTTG
    GGCATCTTCG TTTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023474684.1
    CDS210..6512
    Translation

    Target ORF information:

    RefSeq Version XM_023618916.1
    Organism Equus caballus(horse)
    Definition Equus caballus dysferlin (DYSF), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023618916.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGTGCT GCCTGCTAGT GAAGGCCAGC AACCTTCCCA CTGTGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAA
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG GGTCCCCCTG
    GACCAGAGCT CTGAGCTTCA CGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
    TTCCTGGGAG AAGCCAAGGT CCCACTCCGG GAGGTCCTTG CCACCCCCAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACGCCTCT CCCTGGAGCT GTGCCCCTGT TCCCACCCTC TGTGCCGCTG
    GAACCCTCCC CGACTCTGCC CGATGTGGAT ATGCTGGCTG GTGGGGGACA GAGCCGGGCC
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAATGG
    CAGGCCCCCA CGGATACAGG AGGAGAGGAG GACACCGAGG ACCAGGGGCT CACGGGAGAT
    GAGGCAGAGC CATTCTTGGA TCAGAACGGA GCCCCAGGCC CCGGGGCTCC CACCACCCTG
    AAGAAGCCAC CTTCCCATCC TCCCCCCTAC CATCCTGGGG GGAAAAGGAA GAGAAGCACG
    CCTGCGCCCA GAAAGCTGCT TTCGGATAAA CCACAGGACT TCCAGATCAG GGTCCAGGTG
    ATCGAGGGGC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCGGCC
    AGGCAGACCA AGCGGACTCG GATTCACAGG GGAAACAGCC CGCTCTTCAA CGAGACTCTC
    TTCTTCAACG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGGCCGTCTT TATCACGGTG
    GTAGACTCCT GTTCGCTCCG GACAGATGCC CTCATCGGGG AGTTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCT
    GACGATTTCT CTGCTGGGCC CAAAGGCTAC CTGAAAGCAA GCCTTTGTGT GCTGGGGCCT
    GGAGACGAAG CTCCGCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAGC
    AATCTGCTCA GGCCAACTGG CATGGCCCTT CGAGGAGCGC ACTTCTGTCT GAAGGTCTTC
    AGGGCTGAGG ACTTACCACA GATGGACGAT GCCGTGGTGG ACAGCGTGAA GCAGATCTTC
    GGCTTTGACA GTAACAAGAA GAACCTGGTG GATCCCTTCG TCGAGGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ATGGCCAACC CTCAGTGGAA CCAGAGCATC
    ACGCTGCCTG TCATGTTTCC CTCCATGTCT GAAAAAATGA GGATTCGTGT CATAGACTGG
    GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAATATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGC AGTGGACGAC CACCTGGGCT TCCTCCCCAC CTTCGGGCCC
    TGCTATGTCA ACCTCTACGG CAGCCCCAGG GAGTTCACTG GCTTCCCAGA CCCCTATGCA
    GAGCTCAACA CGGGCAAGGG AGAAGGCGTG GCCTACCGAG GCCGGCTTCT GGTCTCCCTG
    GAGACCAAAC TGGTGGAGCA AGGCGAGCAG AAGGTGGAGG ATCTCGCTGC AGATGACATC
    CTTCGGGTGG AGAAGTACCT CCGGAGGCGC AAGTACTCCC TCTTTGCTGC CTTCTACTCG
    GCCTGCATGC TGCAGGATGT CGACGATGCC ATCCAGTTCG AGGTCAGCAT CGGAAACTAC
    GGGAACAAGT TTGACACGAC CTGCCTGCCG CTGGCCTCCA CCACCCAGTA CAGCCGGGCG
    GTCTTTGACG GGTGCCACTA CTACTACTTA CCCTGGGGCA ACGTGAAGCC TGTGGTGGTG
    CTGTCATCCT ACTGGGAAGA CATCAGCCAC AGAGTCGACA CTCAGAACCA GCTGCTCCGG
    ATCGCCGACC GGCTGGAAGC CGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCGGTGC
    TCCACCGAGG ATGTGGACTC CCTGGTGGCT CAGCTGATGG ATGAGCTCAT TGCAGACTGC
    AGCCAGCCCC TGTGCAACGT CCAAGAGACG CCCTCTGCCA CCCACCTGGA CCAGTACCTG
    TACCGGCTGC GCACCCGTCA CCTGAGCCAG ATCAGTGAGG CGGCCCTGGC CTTGAAGCTC
    GGCCACAGTG AGCTCCCCGC CGCCCTTGAG CAGGCTGAGG ACTGGCTCCT GCGTCTCCGT
    GCCCTGGCGG AGGAGCCCCA GAACAGCCTG CCTGACGTGG TCATCTGGAT GCTGCAGGGA
    GACAAGCGGG TGGCGTACCA GCGGGTGCCC GCCCACGAGG TCCTCTTCTC CCGGCGGGGC
    GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTGCAGACGA TCTTTCTGAA GTATCCAATG
    GAAGGGGTGC CGGGAGCCCG GATGCCGGTG CAGATACGGG TGAAGCTCTG GTTCGGGCTC
    TCTGTGGATG AGAAGGAGTT CAATCAGTTT GCTGAGGGCA AGCTCTCTGT CTTCGCTGAA
    ACCTACGAGA ACCAGACCAA GCTGGCCCTA GTCGGGAACT GGGGCACAAC GGGCCTCACC
    TACCCCAAGT TTTCTGACGT CACAGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC
    TCGGCCGGCT GGGCCTGGGC TGGAGAGTGG TTCGTGTGTC CGGAGAAGAC CCTACTCCAC
    GACACCGATG CGGGCCACCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTG
    CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACAG ATGTGAACGG GGAGAAGGTG
    CTTCCCAAGG ATGACATCGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA AGAATGGTCC
    ACAGACCTCA ATCGGGCCGT TGACGAGCAA GGCTGGGAGT ACAGCATCAC CATCCCTCCG
    GAACGGAAGC CAAGGCACTG GGTCCCTGCC GAGAAGATGT ACTACACACA CCGTCGGCGG
    CGCTGGGTCC GCCTGCGCAG GAGGGACCTC AGCCAGATGG AGGCGCTGAA GAGGCACAGG
    CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCGC TCTTCGGCTG GAAGTTCCAC
    CTGGAGTACC GCAAGACCGA TGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCA
    CTGGAGAAGA CAGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCCCTGGG CGGCGTGGTG
    GACGACAGGA GTGAAGATTC GGTGTCCGTC TCCACCTTGA GCTTCGGCGT GAACAGACCC
    ACGATTTCCT GCATCTTCGA CTACGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG
    GCCCGGGACC TGCCCGCCAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC AATCGTCTCC
    TTCCTGCACC AGAGCCAGAA GACAGTGGTG GTGAAGAACA CCCTGAACCC CACCTGGGAC
    CAGACACTCA TCTTCTATGA GATCGAGATC TTTGGTGAGC CGCCCAGCAT CGCCGAGCAG
    CCGCCCAGCA TCGTGGTGGA GCTATACGAC CATGACACCT ACGGTGCAGA TGAGTTTATG
    GGACGCTGCA TCTGTCAGCC GACTCTGGAA CGGATGCCCC GGCTGACCTG GTTCCCACTG
    ACTAGGGGCA GCCAGCCGGC GGGCGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG
    AAGCCGGCCA TCTACCACAT TCCTGGTTTT GAGGTGCAGG ATACATCAGG GATCCTGGAA
    GAGTCGGAGG ACACAGACCT GCCCTACCCG CCACCCCAGA GAGAGGCCAA CATCTACATG
    GTTCCCCAGA ACATCAAGCC TGCTCTCCAG CGGACGGCCA TCGAGATCCT GGCATGGGGC
    CTGCGGAACA TGAAGAGTTA CCACCTGGCC AGTGTCTCCT CCCCCAGCCT CGTGGTGGAG
    TGCGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCAGAAGAA CCCCAACTTC
    GACGTCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGTCCCCCC
    ATCGTGGTCA AGGTCATCGA TAACTGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT
    ACCATCTGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CAGAGGAGAG TCCGTCCCCA
    CAGGGCGGCC CAGATGATGT GAGCTTACTC AGTCCTGGGG AAGATGTGCT CATTGACATT
    GATGACAAGG AGCCCCTCAT CCCTATGCAG GAGGAGGAGT TCATCGATTG GTGGAGCAAA
    TTCTTTGCCT CGATAGGGGA GAGGGAAAAG TGCGGGTCCT ACTTGGAGAA GGACTTTGAC
    ACTCTAAAGG TCTATGACAC ACAACTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC
    TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGATGGA AGACCCATCT
    GTCGTTGGGG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCGGCCATC
    CCCATGCCCC CAAGACAGTT CCACCAGCTA GCTGCCCAGG GACCCCAGGA GTGCCTGGTC
    CGCATCTACA TCATCCGGGC ATTCGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAC
    CCTTACATCA AGATCTCCAT AGGGAAGAAA TCCGTGAGCG ACCAGGATAA CTACATCCCC
    TGCACTCTGG AGCCTGTGTT TGGAAAGATG TTCGAACTGA CCTGCACTCT GCCTCTGGAG
    AAGGACCTGA AGATCACTCT CTATGACTAT GACCTCCTCT CCAAGGACGA GATGATCGGG
    GAGACGGTCA TCGACCTGGA GAATAGGCTG CTGTCCAAGT TTGGAGCTCG CTGCGGACTC
    CCACAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG
    CTTCTCCACC TGTTCTGCCA GCAGCAGAGA GTCAAGGCCC CCGTGTACCA GACCGACCGT
    GTGGTGTTTG GGAATAAAGA ATACACGATT GAAGAAATAG AGGCTGGGAG GGTCCTGAAT
    CCACACCTGG GCCCAGTGGA AGAGCGCCTG GCCTTGCACG TCCTCCAGCA GCAGGGCTTG
    GTGCCCGAGC ATGTGGAGTC CCGGCCCCTC TATAGCCCCC TGCAGCCGGA CATTGAGCAG
    GGGAAGCTGC AGATGTGGAT CGACCTGTTT CCAAAGGCCC TGGGGCGGCC TGGACCTCCC
    TTCAACATCA CTCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGCATTAT CTGGAACACC
    AAGGATGTGA TCCTGGACGA CCTCAGCATC ACAGGAGAGA AGATGAGCGA CATTTATGTG
    AAAGGTTGGA TGGTTGGCTT TGAAGAACAC AAGCAAAAGA CAGATGTGCA TTATCGGTCC
    CTGGGAGGCG AAGGGAACTT TAACTGGAGG TTCATTTTCC CCTTTGACTA CCTGCTGGCT
    GAGCAAGTCT GCACCGTCTC CAAGAAGGAT GCTTTCTGGA AGCTGGACAA GACCGAGAGC
    AAAATCCCAG CACGAGTGGT ATTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT
    TTCCTGGGCT CCTTGCAGCT GGATCTCAAC CGCATGCCCA AGCCTGCCAA GACAGCCGAG
    AAGTGCTCCC TGGACCAGCT GGATGACACT TTCCACCCAG AGTGGTTTGT GTCCCTTTTT
    GAACAGAAAA CTGTGAAGGG CTGGTGGCCC TGTGTGGCGG AAGAGGGTGA GAAGAAGAAG
    CTGGCGGGCA AGCTGGAGAT GACCTTGGAG ATTGTGGCGG AGAGTGAGCA TGAGGAGCGG
    CCCGCTGGCC AGGGCCGGGA TGAGCCCAAC ATGAACCCCA AGCTCGAGGA CCCAAGGCGC
    CCTGACACCT CCTTCCTCTG GTTCACCTCC CCGTACAAGA CTATGAAGTT CATCCTGTGG
    CGGCGTTTCC GGTGTGCCAT CATCCTCTTC ATCATCCTCT TCATCCTACT GCTGTTCTTG
    GGCATCTTCG TTTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb49785
    Clone ID Related Accession (Same CDS sequence) XM_023618917.1
    Accession Version XM_023618917.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6273bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product dysferlin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009158.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGTGCT GCCTGCTAGT GAAGGCCAGC AACCTTCCCA CTGTGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAA
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG GGTCCCCCTG
    GACCAGAGCT CTGAGCTTCA CGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
    TTCCTGGGAG AAGCCAAGGT CCCACTCCGG GAGGTCCTTG CCACCCCCAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACGCCTCT CCCTGGAGCT GTGCCCCTGT TCCCACCCTC TGTGCCGCTG
    GAACCCTCCC CGACTCTGCC CGATGTGGAT ATGCTGGCTG ATACAGGAGG AGAGGAGGAC
    ACCGAGGACC AGGGGCTCAC GGGAGATGAG GCAGAGCCAT TCTTGGATCA GAACGGAGCC
    CCAGGCCCCG GGGCTCCCAC CACCCTGAAG AAGCCACCTT CCCATCCTCC CCCCTACCAT
    CCTGGGGGGA AAAGGAAGAG AAGCACGCCT GCGCCCAGAA AGCTGCTTTC GGATAAACCA
    CAGGACTTCC AGATCAGGGT CCAGGTGATC GAGGGGCGCC AGCTGCCAGG GGTGAACATC
    AAGCCTGTGG TCAAGGTCAC CGCGGCCAGG CAGACCAAGC GGACTCGGAT TCACAGGGGA
    AACAGCCCGC TCTTCAACGA GACTCTCTTC TTCAACGTGT TTGACTCTCC CTCGGAGCTG
    TTTGACGAGG CCGTCTTTAT CACGGTGGTA GACTCCTGTT CGCTCCGGAC AGATGCCCTC
    ATCGGGGAGT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA CGCCTATCTC
    AGGAAGTGGC TGCTGCTCTC GGACCCTGAC GATTTCTCTG CTGGGCCCAA AGGCTACCTG
    AAAGCAAGCC TTTGTGTGCT GGGGCCTGGA GACGAAGCTC CGCTGGAGAG AAAGGACCCC
    TCTGAAGACA AGGAGGACAT TGAAAGCAAT CTGCTCAGGC CAACTGGCAT GGCCCTTCGA
    GGAGCGCACT TCTGTCTGAA GGTCTTCAGG GCTGAGGACT TACCACAGAT GGACGATGCC
    GTGGTGGACA GCGTGAAGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CCTGGTGGAT
    CCCTTCGTCG AGGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGATG
    GCCAACCCTC AGTGGAACCA GAGCATCACG CTGCCTGTCA TGTTTCCCTC CATGTCTGAA
    AAAATGAGGA TTCGTGTCAT AGACTGGGAC CGCCTCACCC ACAATGACAT CGTGGCCACC
    ACCTACCTGA ATATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGCAGT GGACGACCAC
    CTGGGCTTCC TCCCCACCTT CGGGCCCTGC TATGTCAACC TCTACGGCAG CCCCAGGGAG
    TTCACTGGCT TCCCAGACCC CTATGCAGAG CTCAACACGG GCAAGGGAGA AGGCGTGGCC
    TACCGAGGCC GGCTTCTGGT CTCCCTGGAG ACCAAACTGG TGGAGCAAGG CGAGCAGAAG
    GTGGAGGATC TCGCTGCAGA TGACATCCTT CGGGTGGAGA AGTACCTCCG GAGGCGCAAG
    TACTCCCTCT TTGCTGCCTT CTACTCGGCC TGCATGCTGC AGGATGTCGA CGATGCCATC
    CAGTTCGAGG TCAGCATCGG AAACTACGGG AACAAGTTTG ACACGACCTG CCTGCCGCTG
    GCCTCCACCA CCCAGTACAG CCGGGCGGTC TTTGACGGGT GCCACTACTA CTACTTACCC
    TGGGGCAACG TGAAGCCTGT GGTGGTGCTG TCATCCTACT GGGAAGACAT CAGCCACAGA
    GTCGACACTC AGAACCAGCT GCTCCGGATC GCCGACCGGC TGGAAGCCGG CCTGGAGCAG
    GTCCACCTGG CCCTGAAGGC GCGGTGCTCC ACCGAGGATG TGGACTCCCT GGTGGCTCAG
    CTGATGGATG AGCTCATTGC AGACTGCAGC CAGCCCCTGT GCAACGTCCA AGAGACGCCC
    TCTGCCACCC ACCTGGACCA GTACCTGTAC CGGCTGCGCA CCCGTCACCT GAGCCAGATC
    AGTGAGGCGG CCCTGGCCTT GAAGCTCGGC CACAGTGAGC TCCCCGCCGC CCTTGAGCAG
    GCTGAGGACT GGCTCCTGCG TCTCCGTGCC CTGGCGGAGG AGCCCCAGAA CAGCCTGCCT
    GACGTGGTCA TCTGGATGCT GCAGGGAGAC AAGCGGGTGG CGTACCAGCG GGTGCCCGCC
    CACGAGGTCC TCTTCTCCCG GCGGGGCGCC AACTACTGTG GCAAGAATTG TGGGAAGCTG
    CAGACGATCT TTCTGAAGTA TCCAATGGAA GGGGTGCCGG GAGCCCGGAT GCCGGTGCAG
    ATACGGGTGA AGCTCTGGTT CGGGCTCTCT GTGGATGAGA AGGAGTTCAA TCAGTTTGCT
    GAGGGCAAGC TCTCTGTCTT CGCTGAAACC TACGAGAACC AGACCAAGCT GGCCCTAGTC
    GGGAACTGGG GCACAACGGG CCTCACCTAC CCCAAGTTTT CTGACGTCAC AGGCAAGATC
    AAGCTACCCA AGGACAGCTT CCGCCCCTCG GCCGGCTGGG CCTGGGCTGG AGAGTGGTTC
    GTGTGTCCGG AGAAGACCCT ACTCCACGAC ACCGATGCGG GCCACCTGAG CTTTGTGGAG
    GAGGTGTTTG AGAACCAGAC CCGGCTGCCC GGAGGCCAGT GGATCTACAT GAGTGACAAC
    TACACAGATG TGAACGGGGA GAAGGTGCTT CCCAAGGATG ACATCGAGTG CCCACTGGGC
    TGGAAGTGGG AAGATGAAGA ATGGTCCACA GACCTCAATC GGGCCGTTGA CGAGCAAGGC
    TGGGAGTACA GCATCACCAT CCCTCCGGAA CGGAAGCCAA GGCACTGGGT CCCTGCCGAG
    AAGATGTACT ACACACACCG TCGGCGGCGC TGGGTCCGCC TGCGCAGGAG GGACCTCAGC
    CAGATGGAGG CGCTGAAGAG GCACAGGCAG GCGGAGGCGG AGGGCGAGGG CTGGGAGTAC
    GCCTCGCTCT TCGGCTGGAA GTTCCACCTG GAGTACCGCA AGACCGATGC CTTCCGCCGC
    CGCCGCTGGC GCCGCCGCAT GGAGCCACTG GAGAAGACAG GGCCTGCAGC TGTGTTTGCC
    CTCGAGGGGG CCCTGGGCGG CGTGGTGGAC GACAGGAGTG AAGATTCGGT GTCCGTCTCC
    ACCTTGAGCT TCGGCGTGAA CAGACCCACG ATTTCCTGCA TCTTCGACTA CGGGAACCGC
    TACCATCTAC GCTGCTACAT GTACCAGGCC CGGGACCTGC CCGCCATGGA CAAGGACTCT
    TTTTCTGATC CCTACGCAAT CGTCTCCTTC CTGCACCAGA GCCAGAAGAC AGTGGTGGTG
    AAGAACACCC TGAACCCCAC CTGGGACCAG ACACTCATCT TCTATGAGAT CGAGATCTTT
    GGTGAGCCGC CCAGCATCGC CGAGCAGCCG CCCAGCATCG TGGTGGAGCT ATACGACCAT
    GACACCTACG GTGCAGATGA GTTTATGGGA CGCTGCATCT GTCAGCCGAC TCTGGAACGG
    ATGCCCCGGC TGACCTGGTT CCCACTGACT AGGGGCAGCC AGCCGGCGGG CGAGCTGCTG
    GCCTCTTTTG AGCTCATCCA GAGAGAGAAG CCGGCCATCT ACCACATTCC TGGTTTTGAG
    GTGCAGGATA CATCAGGGAT CCTGGAAGAG TCGGAGGACA CAGACCTGCC CTACCCGCCA
    CCCCAGAGAG AGGCCAACAT CTACATGGTT CCCCAGAACA TCAAGCCTGC TCTCCAGCGG
    ACGGCCATCG AGATCCTGGC ATGGGGCCTG CGGAACATGA AGAGTTACCA CCTGGCCAGT
    GTCTCCTCCC CCAGCCTCGT GGTGGAGTGC GGGGGCCAGA CGGTGCAGTC CTGTGTCATC
    AGGAACCTCC AGAAGAACCC CAACTTCGAC GTCTGCACCC TCTTCATGGA AGTGATGCTG
    CCCAGGGAGG AGCTCTACTG TCCCCCCATC GTGGTCAAGG TCATCGATAA CTGCCAGTTT
    GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCTGCTCCC TGGAGAGCTT CCTGTGTGAC
    CCCTACTCAG AGGAGAGTCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG CTTACTCAGT
    CCTGGGGAAG ATGTGCTCAT TGACATTGAT GACAAGGAGC CCCTCATCCC TATGCAGCTT
    GCAGATGGTC TGTCGGGCTC GGCCCCCACT AACATGGCAT CTTCTCCGTC CAGTCCTCAT
    GAGGAGGAGT TCATCGATTG GTGGAGCAAA TTCTTTGCCT CGATAGGGGA GAGGGAAAAG
    TGCGGGTCCT ACTTGGAGAA GGACTTTGAC ACTCTAAAGG TCTATGACAC ACAACTGGAG
    AATGTGGAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC
    AAGACTCAGG AGGAGATGGA AGACCCATCT GTCGTTGGGG AATTTAAGGG CCTCTTCAAA
    ATTTATCCCC TCCCAGAAGA CCCGGCCATC CCCATGCCCC CAAGACAGTT CCACCAGCTA
    GCTGCCCAGG GACCCCAGGA GTGCCTGGTC CGCATCTACA TCATCCGGGC ATTCGGCCTG
    CAGCCCAAGG ACCCCAATGG GAAGTGTGAC CCTTACATCA AGATCTCCAT AGGGAAGAAA
    TCCGTGAGCG ACCAGGATAA CTACATCCCC TGCACTCTGG AGCCTGTGTT TGGAAAGATG
    TTCGAACTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGATCACTCT CTATGACTAT
    GACCTCCTCT CCAAGGACGA GATGATCGGG GAGACGGTCA TCGACCTGGA GAATAGGCTG
    CTGTCCAAGT TTGGAGCTCG CTGCGGACTC CCACAGACCT ACTGTGTCTC TGGACCGAAC
    CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG CTTCTCCACC TGTTCTGCCA GCAGCAGAGA
    GTCAAGGCCC CCGTGTACCA GACCGACCGT GTGGTGTTTG GGAATAAAGA ATACACGATT
    GAAGAAATAG AGGCTGGGAG GGTCCTGAAT CCACACCTGG GCCCAGTGGA AGAGCGCCTG
    GCCTTGCACG TCCTCCAGCA GCAGGGCTTG GTGCCCGAGC ATGTGGAGTC CCGGCCCCTC
    TATAGCCCCC TGCAGCCGGA CATTGAGCAG GGGAAGCTGC AGATGTGGAT CGACCTGTTT
    CCAAAGGCCC TGGGGCGGCC TGGACCTCCC TTCAACATCA CTCCACGGAG AGCCAGAAGG
    TTTTTCCTGC GTTGCATTAT CTGGAACACC AAGGATGTGA TCCTGGACGA CCTCAGCATC
    ACAGGAGAGA AGATGAGCGA CATTTATGTG AAAGGTTGGA TGGTTGGCTT TGAAGAACAC
    AAGCAAAAGA CAGATGTGCA TTATCGGTCC CTGGGAGGCG AAGGGAACTT TAACTGGAGG
    TTCATTTTCC CCTTTGACTA CCTGCTGGCT GAGCAAGTCT GCACCGTCTC CAAGAAGGAT
    GCTTTCTGGA AGCTGGACAA GACCGAGAGC AAAATCCCAG CACGAGTGGT ATTCCAGATC
    TGGGACAATG ACAAGTTCTC CTTTGATGAT TTCCTGGGCT CCTTGCAGCT GGATCTCAAC
    CGCATGCCCA AGCCTGCCAA GACAGCCGAG AAGTGCTCCC TGGACCAGCT GGATGACACT
    TTCCACCCAG AGTGGTTTGT GTCCCTTTTT GAACAGAAAA CTGTGAAGGG CTGGTGGCCC
    TGTGTGGCGG AAGAGGGTGA GAAGAAGAAG CTGGCGGGCA AGCTGGAGAT GACCTTGGAG
    ATTGTGGCGG AGAGTGAGCA TGAGGAGCGG CCCGCTGGCC AGGGCCGGGA TGAGCCCAAC
    ATGAACCCCA AGCTCGAGGA CCCAAGGCGC CCTGACACCT CCTTCCTCTG GTTCACCTCC
    CCGTACAAGA CTATGAAGTT CATCCTGTGG CGGCGTTTCC GGTGTGCCAT CATCCTCTTC
    ATCATCCTCT TCATCCTACT GCTGTTCTTG GGCATCTTCG TTTACGCCTT CCCGAACTAT
    GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023474685.1
    CDS210..6482
    Translation

    Target ORF information:

    RefSeq Version XM_023618917.1
    Organism Equus caballus(horse)
    Definition Equus caballus dysferlin (DYSF), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023618917.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGTGCT GCCTGCTAGT GAAGGCCAGC AACCTTCCCA CTGTGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAA
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG GGTCCCCCTG
    GACCAGAGCT CTGAGCTTCA CGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
    TTCCTGGGAG AAGCCAAGGT CCCACTCCGG GAGGTCCTTG CCACCCCCAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACGCCTCT CCCTGGAGCT GTGCCCCTGT TCCCACCCTC TGTGCCGCTG
    GAACCCTCCC CGACTCTGCC CGATGTGGAT ATGCTGGCTG ATACAGGAGG AGAGGAGGAC
    ACCGAGGACC AGGGGCTCAC GGGAGATGAG GCAGAGCCAT TCTTGGATCA GAACGGAGCC
    CCAGGCCCCG GGGCTCCCAC CACCCTGAAG AAGCCACCTT CCCATCCTCC CCCCTACCAT
    CCTGGGGGGA AAAGGAAGAG AAGCACGCCT GCGCCCAGAA AGCTGCTTTC GGATAAACCA
    CAGGACTTCC AGATCAGGGT CCAGGTGATC GAGGGGCGCC AGCTGCCAGG GGTGAACATC
    AAGCCTGTGG TCAAGGTCAC CGCGGCCAGG CAGACCAAGC GGACTCGGAT TCACAGGGGA
    AACAGCCCGC TCTTCAACGA GACTCTCTTC TTCAACGTGT TTGACTCTCC CTCGGAGCTG
    TTTGACGAGG CCGTCTTTAT CACGGTGGTA GACTCCTGTT CGCTCCGGAC AGATGCCCTC
    ATCGGGGAGT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA CGCCTATCTC
    AGGAAGTGGC TGCTGCTCTC GGACCCTGAC GATTTCTCTG CTGGGCCCAA AGGCTACCTG
    AAAGCAAGCC TTTGTGTGCT GGGGCCTGGA GACGAAGCTC CGCTGGAGAG AAAGGACCCC
    TCTGAAGACA AGGAGGACAT TGAAAGCAAT CTGCTCAGGC CAACTGGCAT GGCCCTTCGA
    GGAGCGCACT TCTGTCTGAA GGTCTTCAGG GCTGAGGACT TACCACAGAT GGACGATGCC
    GTGGTGGACA GCGTGAAGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CCTGGTGGAT
    CCCTTCGTCG AGGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGATG
    GCCAACCCTC AGTGGAACCA GAGCATCACG CTGCCTGTCA TGTTTCCCTC CATGTCTGAA
    AAAATGAGGA TTCGTGTCAT AGACTGGGAC CGCCTCACCC ACAATGACAT CGTGGCCACC
    ACCTACCTGA ATATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGCAGT GGACGACCAC
    CTGGGCTTCC TCCCCACCTT CGGGCCCTGC TATGTCAACC TCTACGGCAG CCCCAGGGAG
    TTCACTGGCT TCCCAGACCC CTATGCAGAG CTCAACACGG GCAAGGGAGA AGGCGTGGCC
    TACCGAGGCC GGCTTCTGGT CTCCCTGGAG ACCAAACTGG TGGAGCAAGG CGAGCAGAAG
    GTGGAGGATC TCGCTGCAGA TGACATCCTT CGGGTGGAGA AGTACCTCCG GAGGCGCAAG
    TACTCCCTCT TTGCTGCCTT CTACTCGGCC TGCATGCTGC AGGATGTCGA CGATGCCATC
    CAGTTCGAGG TCAGCATCGG AAACTACGGG AACAAGTTTG ACACGACCTG CCTGCCGCTG
    GCCTCCACCA CCCAGTACAG CCGGGCGGTC TTTGACGGGT GCCACTACTA CTACTTACCC
    TGGGGCAACG TGAAGCCTGT GGTGGTGCTG TCATCCTACT GGGAAGACAT CAGCCACAGA
    GTCGACACTC AGAACCAGCT GCTCCGGATC GCCGACCGGC TGGAAGCCGG CCTGGAGCAG
    GTCCACCTGG CCCTGAAGGC GCGGTGCTCC ACCGAGGATG TGGACTCCCT GGTGGCTCAG
    CTGATGGATG AGCTCATTGC AGACTGCAGC CAGCCCCTGT GCAACGTCCA AGAGACGCCC
    TCTGCCACCC ACCTGGACCA GTACCTGTAC CGGCTGCGCA CCCGTCACCT GAGCCAGATC
    AGTGAGGCGG CCCTGGCCTT GAAGCTCGGC CACAGTGAGC TCCCCGCCGC CCTTGAGCAG
    GCTGAGGACT GGCTCCTGCG TCTCCGTGCC CTGGCGGAGG AGCCCCAGAA CAGCCTGCCT
    GACGTGGTCA TCTGGATGCT GCAGGGAGAC AAGCGGGTGG CGTACCAGCG GGTGCCCGCC
    CACGAGGTCC TCTTCTCCCG GCGGGGCGCC AACTACTGTG GCAAGAATTG TGGGAAGCTG
    CAGACGATCT TTCTGAAGTA TCCAATGGAA GGGGTGCCGG GAGCCCGGAT GCCGGTGCAG
    ATACGGGTGA AGCTCTGGTT CGGGCTCTCT GTGGATGAGA AGGAGTTCAA TCAGTTTGCT
    GAGGGCAAGC TCTCTGTCTT CGCTGAAACC TACGAGAACC AGACCAAGCT GGCCCTAGTC
    GGGAACTGGG GCACAACGGG CCTCACCTAC CCCAAGTTTT CTGACGTCAC AGGCAAGATC
    AAGCTACCCA AGGACAGCTT CCGCCCCTCG GCCGGCTGGG CCTGGGCTGG AGAGTGGTTC
    GTGTGTCCGG AGAAGACCCT ACTCCACGAC ACCGATGCGG GCCACCTGAG CTTTGTGGAG
    GAGGTGTTTG AGAACCAGAC CCGGCTGCCC GGAGGCCAGT GGATCTACAT GAGTGACAAC
    TACACAGATG TGAACGGGGA GAAGGTGCTT CCCAAGGATG ACATCGAGTG CCCACTGGGC
    TGGAAGTGGG AAGATGAAGA ATGGTCCACA GACCTCAATC GGGCCGTTGA CGAGCAAGGC
    TGGGAGTACA GCATCACCAT CCCTCCGGAA CGGAAGCCAA GGCACTGGGT CCCTGCCGAG
    AAGATGTACT ACACACACCG TCGGCGGCGC TGGGTCCGCC TGCGCAGGAG GGACCTCAGC
    CAGATGGAGG CGCTGAAGAG GCACAGGCAG GCGGAGGCGG AGGGCGAGGG CTGGGAGTAC
    GCCTCGCTCT TCGGCTGGAA GTTCCACCTG GAGTACCGCA AGACCGATGC CTTCCGCCGC
    CGCCGCTGGC GCCGCCGCAT GGAGCCACTG GAGAAGACAG GGCCTGCAGC TGTGTTTGCC
    CTCGAGGGGG CCCTGGGCGG CGTGGTGGAC GACAGGAGTG AAGATTCGGT GTCCGTCTCC
    ACCTTGAGCT TCGGCGTGAA CAGACCCACG ATTTCCTGCA TCTTCGACTA CGGGAACCGC
    TACCATCTAC GCTGCTACAT GTACCAGGCC CGGGACCTGC CCGCCATGGA CAAGGACTCT
    TTTTCTGATC CCTACGCAAT CGTCTCCTTC CTGCACCAGA GCCAGAAGAC AGTGGTGGTG
    AAGAACACCC TGAACCCCAC CTGGGACCAG ACACTCATCT TCTATGAGAT CGAGATCTTT
    GGTGAGCCGC CCAGCATCGC CGAGCAGCCG CCCAGCATCG TGGTGGAGCT ATACGACCAT
    GACACCTACG GTGCAGATGA GTTTATGGGA CGCTGCATCT GTCAGCCGAC TCTGGAACGG
    ATGCCCCGGC TGACCTGGTT CCCACTGACT AGGGGCAGCC AGCCGGCGGG CGAGCTGCTG
    GCCTCTTTTG AGCTCATCCA GAGAGAGAAG CCGGCCATCT ACCACATTCC TGGTTTTGAG
    GTGCAGGATA CATCAGGGAT CCTGGAAGAG TCGGAGGACA CAGACCTGCC CTACCCGCCA
    CCCCAGAGAG AGGCCAACAT CTACATGGTT CCCCAGAACA TCAAGCCTGC TCTCCAGCGG
    ACGGCCATCG AGATCCTGGC ATGGGGCCTG CGGAACATGA AGAGTTACCA CCTGGCCAGT
    GTCTCCTCCC CCAGCCTCGT GGTGGAGTGC GGGGGCCAGA CGGTGCAGTC CTGTGTCATC
    AGGAACCTCC AGAAGAACCC CAACTTCGAC GTCTGCACCC TCTTCATGGA AGTGATGCTG
    CCCAGGGAGG AGCTCTACTG TCCCCCCATC GTGGTCAAGG TCATCGATAA CTGCCAGTTT
    GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCTGCTCCC TGGAGAGCTT CCTGTGTGAC
    CCCTACTCAG AGGAGAGTCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG CTTACTCAGT
    CCTGGGGAAG ATGTGCTCAT TGACATTGAT GACAAGGAGC CCCTCATCCC TATGCAGCTT
    GCAGATGGTC TGTCGGGCTC GGCCCCCACT AACATGGCAT CTTCTCCGTC CAGTCCTCAT
    GAGGAGGAGT TCATCGATTG GTGGAGCAAA TTCTTTGCCT CGATAGGGGA GAGGGAAAAG
    TGCGGGTCCT ACTTGGAGAA GGACTTTGAC ACTCTAAAGG TCTATGACAC ACAACTGGAG
    AATGTGGAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC
    AAGACTCAGG AGGAGATGGA AGACCCATCT GTCGTTGGGG AATTTAAGGG CCTCTTCAAA
    ATTTATCCCC TCCCAGAAGA CCCGGCCATC CCCATGCCCC CAAGACAGTT CCACCAGCTA
    GCTGCCCAGG GACCCCAGGA GTGCCTGGTC CGCATCTACA TCATCCGGGC ATTCGGCCTG
    CAGCCCAAGG ACCCCAATGG GAAGTGTGAC CCTTACATCA AGATCTCCAT AGGGAAGAAA
    TCCGTGAGCG ACCAGGATAA CTACATCCCC TGCACTCTGG AGCCTGTGTT TGGAAAGATG
    TTCGAACTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGATCACTCT CTATGACTAT
    GACCTCCTCT CCAAGGACGA GATGATCGGG GAGACGGTCA TCGACCTGGA GAATAGGCTG
    CTGTCCAAGT TTGGAGCTCG CTGCGGACTC CCACAGACCT ACTGTGTCTC TGGACCGAAC
    CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG CTTCTCCACC TGTTCTGCCA GCAGCAGAGA
    GTCAAGGCCC CCGTGTACCA GACCGACCGT GTGGTGTTTG GGAATAAAGA ATACACGATT
    GAAGAAATAG AGGCTGGGAG GGTCCTGAAT CCACACCTGG GCCCAGTGGA AGAGCGCCTG
    GCCTTGCACG TCCTCCAGCA GCAGGGCTTG GTGCCCGAGC ATGTGGAGTC CCGGCCCCTC
    TATAGCCCCC TGCAGCCGGA CATTGAGCAG GGGAAGCTGC AGATGTGGAT CGACCTGTTT
    CCAAAGGCCC TGGGGCGGCC TGGACCTCCC TTCAACATCA CTCCACGGAG AGCCAGAAGG
    TTTTTCCTGC GTTGCATTAT CTGGAACACC AAGGATGTGA TCCTGGACGA CCTCAGCATC
    ACAGGAGAGA AGATGAGCGA CATTTATGTG AAAGGTTGGA TGGTTGGCTT TGAAGAACAC
    AAGCAAAAGA CAGATGTGCA TTATCGGTCC CTGGGAGGCG AAGGGAACTT TAACTGGAGG
    TTCATTTTCC CCTTTGACTA CCTGCTGGCT GAGCAAGTCT GCACCGTCTC CAAGAAGGAT
    GCTTTCTGGA AGCTGGACAA GACCGAGAGC AAAATCCCAG CACGAGTGGT ATTCCAGATC
    TGGGACAATG ACAAGTTCTC CTTTGATGAT TTCCTGGGCT CCTTGCAGCT GGATCTCAAC
    CGCATGCCCA AGCCTGCCAA GACAGCCGAG AAGTGCTCCC TGGACCAGCT GGATGACACT
    TTCCACCCAG AGTGGTTTGT GTCCCTTTTT GAACAGAAAA CTGTGAAGGG CTGGTGGCCC
    TGTGTGGCGG AAGAGGGTGA GAAGAAGAAG CTGGCGGGCA AGCTGGAGAT GACCTTGGAG
    ATTGTGGCGG AGAGTGAGCA TGAGGAGCGG CCCGCTGGCC AGGGCCGGGA TGAGCCCAAC
    ATGAACCCCA AGCTCGAGGA CCCAAGGCGC CCTGACACCT CCTTCCTCTG GTTCACCTCC
    CCGTACAAGA CTATGAAGTT CATCCTGTGG CGGCGTTTCC GGTGTGCCAT CATCCTCTTC
    ATCATCCTCT TCATCCTACT GCTGTTCTTG GGCATCTTCG TTTACGCCTT CCCGAACTAT
    GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb49786
    Clone ID Related Accession (Same CDS sequence) XM_023618918.1
    Accession Version XM_023618918.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product dysferlin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009158.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGTGCT GCCTGCTAGT GAAGGCCAGC AACCTTCCCA CTGTGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAA
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG GGTCCCCCTG
    GACCAGAGCT CTGAGCTTCA CGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
    TTCCTGGGAG AAGCCAAGGT CCCACTCCGG GAGGTCCTTG CCACCCCCAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACGCCTCT CCCTGGAGCT GTGCCCCTGT TCCCACCCTC TGTGCCGCTG
    GAACCCTCCC CGACTCTGCC CGATGTGGAT ATGCTGGCTG ATACAGGAGG AGAGGAGGAC
    ACCGAGGACC AGGGGCTCAC GGGAGATGAG GCAGAGCCAT TCTTGGATCA GAACGGAGCC
    CCAGGCCCCG GGGCTCCCAC CACCCTGAAG AAGCCACCTT CCCATCCTCC CCCCTACCAT
    CCTGGGGGGA AAAGGAAGAG AAGCACGCCT GCGCCCAGAA AGCTGCTTTC GGATAAACCA
    CAGGACTTCC AGATCAGGGT CCAGGTGATC GAGGGGCGCC AGCTGCCAGG GGTGAACATC
    AAGCCTGTGG TCAAGGTCAC CGCGGCCAGG CAGACCAAGC GGACTCGGAT TCACAGGGGA
    AACAGCCCGC TCTTCAACGA GACTCTCTTC TTCAACGTGT TTGACTCTCC CTCGGAGCTG
    TTTGACGAGG CCGTCTTTAT CACGGTGGTA GACTCCTGTT CGCTCCGGAC AGATGCCCTC
    ATCGGGGAGT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA CGCCTATCTC
    AGGAAGTGGC TGCTGCTCTC GGACCCTGAC GATTTCTCTG CTGGGCCCAA AGGCTACCTG
    AAAGCAAGCC TTTGTGTGCT GGGGCCTGGA GACGAAGCTC CGCTGGAGAG AAAGGACCCC
    TCTGAAGACA AGGAGGACAT TGAAAGCAAT CTGCTCAGGC CAACTGGCAT GGCCCTTCGA
    GGAGCGCACT TCTGTCTGAA GGTCTTCAGG GCTGAGGACT TACCACAGAT GGACGATGCC
    GTGGTGGACA GCGTGAAGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CCTGGTGGAT
    CCCTTCGTCG AGGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGATG
    GCCAACCCTC AGTGGAACCA GAGCATCACG CTGCCTGTCA TGTTTCCCTC CATGTCTGAA
    AAAATGAGGA TTCGTGTCAT AGACTGGGAC CGCCTCACCC ACAATGACAT CGTGGCCACC
    ACCTACCTGA ATATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGCAGA GGAGCCTGCA
    GGTGTGGTCA AGCCTCCTCC AGCCACGGAC CTGGACGACC ACCTGGGCTT CCTCCCCACC
    TTCGGGCCCT GCTATGTCAA CCTCTACGGC AGCCCCAGGG AGTTCACTGG CTTCCCAGAC
    CCCTATGCAG AGCTCAACAC GGGCAAGGGA GAAGGCGTGG CCTACCGAGG CCGGCTTCTG
    GTCTCCCTGG AGACCAAACT GGTGGAGCAA GGCGAGCAGA AGGTGGAGGA TCTCGCTGCA
    GATGACATCC TTCGGGTGGA GAAGTACCTC CGGAGGCGCA AGTACTCCCT CTTTGCTGCC
    TTCTACTCGG CCTGCATGCT GCAGGATGTC GACGATGCCA TCCAGTTCGA GGTCAGCATC
    GGAAACTACG GGAACAAGTT TGACACGACC TGCCTGCCGC TGGCCTCCAC CACCCAGTAC
    AGCCGGGCGG TCTTTGACGG GTGCCACTAC TACTACTTAC CCTGGGGCAA CGTGAAGCCT
    GTGGTGGTGC TGTCATCCTA CTGGGAAGAC ATCAGCCACA GAGTCGACAC TCAGAACCAG
    CTGCTCCGGA TCGCCGACCG GCTGGAAGCC GGCCTGGAGC AGGTCCACCT GGCCCTGAAG
    GCGCGGTGCT CCACCGAGGA TGTGGACTCC CTGGTGGCTC AGCTGATGGA TGAGCTCATT
    GCAGACTGCA GCCAGCCCCT GTGCAACGTC CAAGAGACGC CCTCTGCCAC CCACCTGGAC
    CAGTACCTGT ACCGGCTGCG CACCCGTCAC CTGAGCCAGA TCAGTGAGGC GGCCCTGGCC
    TTGAAGCTCG GCCACAGTGA GCTCCCCGCC GCCCTTGAGC AGGCTGAGGA CTGGCTCCTG
    CGTCTCCGTG CCCTGGCGGA GGAGCCCCAG AACAGCCTGC CTGACGTGGT CATCTGGATG
    CTGCAGGGAG ACAAGCGGGT GGCGTACCAG CGGGTGCCCG CCCACGAGGT CCTCTTCTCC
    CGGCGGGGCG CCAACTACTG TGGCAAGAAT TGTGGGAAGC TGCAGACGAT CTTTCTGAAG
    TATCCAATGG AAGGGGTGCC GGGAGCCCGG ATGCCGGTGC AGATACGGGT GAAGCTCTGG
    TTCGGGCTCT CTGTGGATGA GAAGGAGTTC AATCAGTTTG CTGAGGGCAA GCTCTCTGTC
    TTCGCTGAAA CCTACGAGAA CCAGACCAAG CTGGCCCTAG TCGGGAACTG GGGCACAACG
    GGCCTCACCT ACCCCAAGTT TTCTGACGTC ACAGGCAAGA TCAAGCTACC CAAGGACAGC
    TTCCGCCCCT CGGCCGGCTG GGCCTGGGCT GGAGAGTGGT TCGTGTGTCC GGAGAAGACC
    CTACTCCACG ACACCGATGC GGGCCACCTG AGCTTTGTGG AGGAGGTGTT TGAGAACCAG
    ACCCGGCTGC CCGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACAGA TGTGAACGGG
    GAGAAGGTGC TTCCCAAGGA TGACATCGAG TGCCCACTGG GCTGGAAGTG GGAAGATGAA
    GAATGGTCCA CAGACCTCAA TCGGGCCGTT GACGAGCAAG GCTGGGAGTA CAGCATCACC
    ATCCCTCCGG AACGGAAGCC AAGGCACTGG GTCCCTGCCG AGAAGATGTA CTACACACAC
    CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG AGGGACCTCA GCCAGATGGA GGCGCTGAAG
    AGGCACAGGC AGGCGGAGGC GGAGGGCGAG GGCTGGGAGT ACGCCTCGCT CTTCGGCTGG
    AAGTTCCACC TGGAGTACCG CAAGACCGAT GCCTTCCGCC GCCGCCGCTG GCGCCGCCGC
    ATGGAGCCAC TGGAGAAGAC AGGGCCTGCA GCTGTGTTTG CCCTCGAGGG GGCCCTGGGC
    GGCGTGGTGG ACGACAGGAG TGAAGATTCG GTGTCCGTCT CCACCTTGAG CTTCGGCGTG
    AACAGACCCA CGATTTCCTG CATCTTCGAC TACGGGAACC GCTACCATCT ACGCTGCTAC
    ATGTACCAGG CCCGGGACCT GCCCGCCATG GACAAGGACT CTTTTTCTGA TCCCTACGCA
    ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG TGAAGAACAC CCTGAACCCC
    ACCTGGGACC AGACACTCAT CTTCTATGAG ATCGAGATCT TTGGTGAGCC GCCCAGCATC
    GCCGAGCAGC CGCCCAGCAT CGTGGTGGAG CTATACGACC ATGACACCTA CGGTGCAGAT
    GAGTTTATGG GACGCTGCAT CTGTCAGCCG ACTCTGGAAC GGATGCCCCG GCTGACCTGG
    TTCCCACTGA CTAGGGGCAG CCAGCCGGCG GGCGAGCTGC TGGCCTCTTT TGAGCTCATC
    CAGAGAGAGA AGCCGGCCAT CTACCACATT CCTGGTTTTG AGGTGCAGGA TACATCAGGG
    ATCCTGGAAG AGTCGGAGGA CACAGACCTG CCCTACCCGC CACCCCAGAG AGAGGCCAAC
    ATCTACATGG TTCCCCAGAA CATCAAGCCT GCTCTCCAGC GGACGGCCAT CGAGATCCTG
    GCATGGGGCC TGCGGAACAT GAAGAGTTAC CACCTGGCCA GTGTCTCCTC CCCCAGCCTC
    GTGGTGGAGT GCGGGGGCCA GACGGTGCAG TCCTGTGTCA TCAGGAACCT CCAGAAGAAC
    CCCAACTTCG ACGTCTGCAC CCTCTTCATG GAAGTGATGC TGCCCAGGGA GGAGCTCTAC
    TGTCCCCCCA TCGTGGTCAA GGTCATCGAT AACTGCCAGT TTGGCCGCCG GCCTGTGGTG
    GGCCAGTGTA CCATCTGCTC CCTGGAGAGC TTCCTGTGTG ACCCCTACTC AGAGGAGAGT
    CCGTCCCCAC AGGGCGGCCC AGATGATGTG AGCTTACTCA GTCCTGGGGA AGATGTGCTC
    ATTGACATTG ATGACAAGGA GCCCCTCATC CCTATGCAGG AGGAGGAGTT CATCGATTGG
    TGGAGCAAAT TCTTTGCCTC GATAGGGGAG AGGGAAAAGT GCGGGTCCTA CTTGGAGAAG
    GACTTTGACA CTCTAAAGGT CTATGACACA CAACTGGAGA ATGTGGAGGC CTTTGAGGGC
    CTGTCTGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA AGACTCAGGA GGAGATGGAA
    GACCCATCTG TCGTTGGGGA ATTTAAGGGC CTCTTCAAAA TTTATCCCCT CCCAGAAGAC
    CCGGCCATCC CCATGCCCCC AAGACAGTTC CACCAGCTAG CTGCCCAGGG ACCCCAGGAG
    TGCCTGGTCC GCATCTACAT CATCCGGGCA TTCGGCCTGC AGCCCAAGGA CCCCAATGGG
    AAGTGTGACC CTTACATCAA GATCTCCATA GGGAAGAAAT CCGTGAGCGA CCAGGATAAC
    TACATCCCCT GCACTCTGGA GCCTGTGTTT GGAAAGATGT TCGAACTGAC CTGCACTCTG
    CCTCTGGAGA AGGACCTGAA GATCACTCTC TATGACTATG ACCTCCTCTC CAAGGACGAG
    ATGATCGGGG AGACGGTCAT CGACCTGGAG AATAGGCTGC TGTCCAAGTT TGGAGCTCGC
    TGCGGACTCC CACAGACCTA CTGTGTCTCT GGACCGAACC AGTGGAGGGA CCAGCTCCGC
    CCCTCCCAGC TTCTCCACCT GTTCTGCCAG CAGCAGAGAG TCAAGGCCCC CGTGTACCAG
    ACCGACCGTG TGGTGTTTGG GAATAAAGAA TACACGATTG AAGAAATAGA GGCTGGGAGG
    GTCCTGAATC CACACCTGGG CCCAGTGGAA GAGCGCCTGG CCTTGCACGT CCTCCAGCAG
    CAGGGCTTGG TGCCCGAGCA TGTGGAGTCC CGGCCCCTCT ATAGCCCCCT GCAGCCGGAC
    ATTGAGCAGG GGAAGCTGCA GATGTGGATC GACCTGTTTC CAAAGGCCCT GGGGCGGCCT
    GGACCTCCCT TCAACATCAC TCCACGGAGA GCCAGAAGGT TTTTCCTGCG TTGCATTATC
    TGGAACACCA AGGATGTGAT CCTGGACGAC CTCAGCATCA CAGGAGAGAA GATGAGCGAC
    ATTTATGTGA AAGGTTGGAT GGTTGGCTTT GAAGAACACA AGCAAAAGAC AGATGTGCAT
    TATCGGTCCC TGGGAGGCGA AGGGAACTTT AACTGGAGGT TCATTTTCCC CTTTGACTAC
    CTGCTGGCTG AGCAAGTCTG CACCGTCTCC AAGAAGGATG CTTTCTGGAA GCTGGACAAG
    ACCGAGAGCA AAATCCCAGC ACGAGTGGTA TTCCAGATCT GGGACAATGA CAAGTTCTCC
    TTTGATGATT TCCTGGGCTC CTTGCAGCTG GATCTCAACC GCATGCCCAA GCCTGCCAAG
    ACAGCCGAGA AGTGCTCCCT GGACCAGCTG GATGACACTT TCCACCCAGA GTGGTTTGTG
    TCCCTTTTTG AACAGAAAAC TGTGAAGGGC TGGTGGCCCT GTGTGGCGGA AGAGGGTGAG
    AAGAAGAAGC TGGCGGGCAA GCTGGAGATG ACCTTGGAGA TTGTGGCGGA GAGTGAGCAT
    GAGGAGCGGC CCGCTGGCCA GGGCCGGGAT GAGCCCAACA TGAACCCCAA GCTCGAGGAC
    CCAAGGCGCC CTGACACCTC CTTCCTCTGG TTCACCTCCC CGTACAAGAC TATGAAGTTC
    ATCCTGTGGC GGCGTTTCCG GTGTGCCATC ATCCTCTTCA TCATCCTCTT CATCCTACTG
    CTGTTCTTGG GCATCTTCGT TTACGCCTTC CCGAACTATG CTGCCATGAA GCTGGTGAAG
    CCCTTCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023474686.1
    CDS210..6461
    Translation

    Target ORF information:

    RefSeq Version XM_023618918.1
    Organism Equus caballus(horse)
    Definition Equus caballus dysferlin (DYSF), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023618918.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGTGCT GCCTGCTAGT GAAGGCCAGC AACCTTCCCA CTGTGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAA
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG GGTCCCCCTG
    GACCAGAGCT CTGAGCTTCA CGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
    TTCCTGGGAG AAGCCAAGGT CCCACTCCGG GAGGTCCTTG CCACCCCCAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACGCCTCT CCCTGGAGCT GTGCCCCTGT TCCCACCCTC TGTGCCGCTG
    GAACCCTCCC CGACTCTGCC CGATGTGGAT ATGCTGGCTG ATACAGGAGG AGAGGAGGAC
    ACCGAGGACC AGGGGCTCAC GGGAGATGAG GCAGAGCCAT TCTTGGATCA GAACGGAGCC
    CCAGGCCCCG GGGCTCCCAC CACCCTGAAG AAGCCACCTT CCCATCCTCC CCCCTACCAT
    CCTGGGGGGA AAAGGAAGAG AAGCACGCCT GCGCCCAGAA AGCTGCTTTC GGATAAACCA
    CAGGACTTCC AGATCAGGGT CCAGGTGATC GAGGGGCGCC AGCTGCCAGG GGTGAACATC
    AAGCCTGTGG TCAAGGTCAC CGCGGCCAGG CAGACCAAGC GGACTCGGAT TCACAGGGGA
    AACAGCCCGC TCTTCAACGA GACTCTCTTC TTCAACGTGT TTGACTCTCC CTCGGAGCTG
    TTTGACGAGG CCGTCTTTAT CACGGTGGTA GACTCCTGTT CGCTCCGGAC AGATGCCCTC
    ATCGGGGAGT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA CGCCTATCTC
    AGGAAGTGGC TGCTGCTCTC GGACCCTGAC GATTTCTCTG CTGGGCCCAA AGGCTACCTG
    AAAGCAAGCC TTTGTGTGCT GGGGCCTGGA GACGAAGCTC CGCTGGAGAG AAAGGACCCC
    TCTGAAGACA AGGAGGACAT TGAAAGCAAT CTGCTCAGGC CAACTGGCAT GGCCCTTCGA
    GGAGCGCACT TCTGTCTGAA GGTCTTCAGG GCTGAGGACT TACCACAGAT GGACGATGCC
    GTGGTGGACA GCGTGAAGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CCTGGTGGAT
    CCCTTCGTCG AGGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGATG
    GCCAACCCTC AGTGGAACCA GAGCATCACG CTGCCTGTCA TGTTTCCCTC CATGTCTGAA
    AAAATGAGGA TTCGTGTCAT AGACTGGGAC CGCCTCACCC ACAATGACAT CGTGGCCACC
    ACCTACCTGA ATATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGCAGA GGAGCCTGCA
    GGTGTGGTCA AGCCTCCTCC AGCCACGGAC CTGGACGACC ACCTGGGCTT CCTCCCCACC
    TTCGGGCCCT GCTATGTCAA CCTCTACGGC AGCCCCAGGG AGTTCACTGG CTTCCCAGAC
    CCCTATGCAG AGCTCAACAC GGGCAAGGGA GAAGGCGTGG CCTACCGAGG CCGGCTTCTG
    GTCTCCCTGG AGACCAAACT GGTGGAGCAA GGCGAGCAGA AGGTGGAGGA TCTCGCTGCA
    GATGACATCC TTCGGGTGGA GAAGTACCTC CGGAGGCGCA AGTACTCCCT CTTTGCTGCC
    TTCTACTCGG CCTGCATGCT GCAGGATGTC GACGATGCCA TCCAGTTCGA GGTCAGCATC
    GGAAACTACG GGAACAAGTT TGACACGACC TGCCTGCCGC TGGCCTCCAC CACCCAGTAC
    AGCCGGGCGG TCTTTGACGG GTGCCACTAC TACTACTTAC CCTGGGGCAA CGTGAAGCCT
    GTGGTGGTGC TGTCATCCTA CTGGGAAGAC ATCAGCCACA GAGTCGACAC TCAGAACCAG
    CTGCTCCGGA TCGCCGACCG GCTGGAAGCC GGCCTGGAGC AGGTCCACCT GGCCCTGAAG
    GCGCGGTGCT CCACCGAGGA TGTGGACTCC CTGGTGGCTC AGCTGATGGA TGAGCTCATT
    GCAGACTGCA GCCAGCCCCT GTGCAACGTC CAAGAGACGC CCTCTGCCAC CCACCTGGAC
    CAGTACCTGT ACCGGCTGCG CACCCGTCAC CTGAGCCAGA TCAGTGAGGC GGCCCTGGCC
    TTGAAGCTCG GCCACAGTGA GCTCCCCGCC GCCCTTGAGC AGGCTGAGGA CTGGCTCCTG
    CGTCTCCGTG CCCTGGCGGA GGAGCCCCAG AACAGCCTGC CTGACGTGGT CATCTGGATG
    CTGCAGGGAG ACAAGCGGGT GGCGTACCAG CGGGTGCCCG CCCACGAGGT CCTCTTCTCC
    CGGCGGGGCG CCAACTACTG TGGCAAGAAT TGTGGGAAGC TGCAGACGAT CTTTCTGAAG
    TATCCAATGG AAGGGGTGCC GGGAGCCCGG ATGCCGGTGC AGATACGGGT GAAGCTCTGG
    TTCGGGCTCT CTGTGGATGA GAAGGAGTTC AATCAGTTTG CTGAGGGCAA GCTCTCTGTC
    TTCGCTGAAA CCTACGAGAA CCAGACCAAG CTGGCCCTAG TCGGGAACTG GGGCACAACG
    GGCCTCACCT ACCCCAAGTT TTCTGACGTC ACAGGCAAGA TCAAGCTACC CAAGGACAGC
    TTCCGCCCCT CGGCCGGCTG GGCCTGGGCT GGAGAGTGGT TCGTGTGTCC GGAGAAGACC
    CTACTCCACG ACACCGATGC GGGCCACCTG AGCTTTGTGG AGGAGGTGTT TGAGAACCAG
    ACCCGGCTGC CCGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACAGA TGTGAACGGG
    GAGAAGGTGC TTCCCAAGGA TGACATCGAG TGCCCACTGG GCTGGAAGTG GGAAGATGAA
    GAATGGTCCA CAGACCTCAA TCGGGCCGTT GACGAGCAAG GCTGGGAGTA CAGCATCACC
    ATCCCTCCGG AACGGAAGCC AAGGCACTGG GTCCCTGCCG AGAAGATGTA CTACACACAC
    CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG AGGGACCTCA GCCAGATGGA GGCGCTGAAG
    AGGCACAGGC AGGCGGAGGC GGAGGGCGAG GGCTGGGAGT ACGCCTCGCT CTTCGGCTGG
    AAGTTCCACC TGGAGTACCG CAAGACCGAT GCCTTCCGCC GCCGCCGCTG GCGCCGCCGC
    ATGGAGCCAC TGGAGAAGAC AGGGCCTGCA GCTGTGTTTG CCCTCGAGGG GGCCCTGGGC
    GGCGTGGTGG ACGACAGGAG TGAAGATTCG GTGTCCGTCT CCACCTTGAG CTTCGGCGTG
    AACAGACCCA CGATTTCCTG CATCTTCGAC TACGGGAACC GCTACCATCT ACGCTGCTAC
    ATGTACCAGG CCCGGGACCT GCCCGCCATG GACAAGGACT CTTTTTCTGA TCCCTACGCA
    ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG TGAAGAACAC CCTGAACCCC
    ACCTGGGACC AGACACTCAT CTTCTATGAG ATCGAGATCT TTGGTGAGCC GCCCAGCATC
    GCCGAGCAGC CGCCCAGCAT CGTGGTGGAG CTATACGACC ATGACACCTA CGGTGCAGAT
    GAGTTTATGG GACGCTGCAT CTGTCAGCCG ACTCTGGAAC GGATGCCCCG GCTGACCTGG
    TTCCCACTGA CTAGGGGCAG CCAGCCGGCG GGCGAGCTGC TGGCCTCTTT TGAGCTCATC
    CAGAGAGAGA AGCCGGCCAT CTACCACATT CCTGGTTTTG AGGTGCAGGA TACATCAGGG
    ATCCTGGAAG AGTCGGAGGA CACAGACCTG CCCTACCCGC CACCCCAGAG AGAGGCCAAC
    ATCTACATGG TTCCCCAGAA CATCAAGCCT GCTCTCCAGC GGACGGCCAT CGAGATCCTG
    GCATGGGGCC TGCGGAACAT GAAGAGTTAC CACCTGGCCA GTGTCTCCTC CCCCAGCCTC
    GTGGTGGAGT GCGGGGGCCA GACGGTGCAG TCCTGTGTCA TCAGGAACCT CCAGAAGAAC
    CCCAACTTCG ACGTCTGCAC CCTCTTCATG GAAGTGATGC TGCCCAGGGA GGAGCTCTAC
    TGTCCCCCCA TCGTGGTCAA GGTCATCGAT AACTGCCAGT TTGGCCGCCG GCCTGTGGTG
    GGCCAGTGTA CCATCTGCTC CCTGGAGAGC TTCCTGTGTG ACCCCTACTC AGAGGAGAGT
    CCGTCCCCAC AGGGCGGCCC AGATGATGTG AGCTTACTCA GTCCTGGGGA AGATGTGCTC
    ATTGACATTG ATGACAAGGA GCCCCTCATC CCTATGCAGG AGGAGGAGTT CATCGATTGG
    TGGAGCAAAT TCTTTGCCTC GATAGGGGAG AGGGAAAAGT GCGGGTCCTA CTTGGAGAAG
    GACTTTGACA CTCTAAAGGT CTATGACACA CAACTGGAGA ATGTGGAGGC CTTTGAGGGC
    CTGTCTGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA AGACTCAGGA GGAGATGGAA
    GACCCATCTG TCGTTGGGGA ATTTAAGGGC CTCTTCAAAA TTTATCCCCT CCCAGAAGAC
    CCGGCCATCC CCATGCCCCC AAGACAGTTC CACCAGCTAG CTGCCCAGGG ACCCCAGGAG
    TGCCTGGTCC GCATCTACAT CATCCGGGCA TTCGGCCTGC AGCCCAAGGA CCCCAATGGG
    AAGTGTGACC CTTACATCAA GATCTCCATA GGGAAGAAAT CCGTGAGCGA CCAGGATAAC
    TACATCCCCT GCACTCTGGA GCCTGTGTTT GGAAAGATGT TCGAACTGAC CTGCACTCTG
    CCTCTGGAGA AGGACCTGAA GATCACTCTC TATGACTATG ACCTCCTCTC CAAGGACGAG
    ATGATCGGGG AGACGGTCAT CGACCTGGAG AATAGGCTGC TGTCCAAGTT TGGAGCTCGC
    TGCGGACTCC CACAGACCTA CTGTGTCTCT GGACCGAACC AGTGGAGGGA CCAGCTCCGC
    CCCTCCCAGC TTCTCCACCT GTTCTGCCAG CAGCAGAGAG TCAAGGCCCC CGTGTACCAG
    ACCGACCGTG TGGTGTTTGG GAATAAAGAA TACACGATTG AAGAAATAGA GGCTGGGAGG
    GTCCTGAATC CACACCTGGG CCCAGTGGAA GAGCGCCTGG CCTTGCACGT CCTCCAGCAG
    CAGGGCTTGG TGCCCGAGCA TGTGGAGTCC CGGCCCCTCT ATAGCCCCCT GCAGCCGGAC
    ATTGAGCAGG GGAAGCTGCA GATGTGGATC GACCTGTTTC CAAAGGCCCT GGGGCGGCCT
    GGACCTCCCT TCAACATCAC TCCACGGAGA GCCAGAAGGT TTTTCCTGCG TTGCATTATC
    TGGAACACCA AGGATGTGAT CCTGGACGAC CTCAGCATCA CAGGAGAGAA GATGAGCGAC
    ATTTATGTGA AAGGTTGGAT GGTTGGCTTT GAAGAACACA AGCAAAAGAC AGATGTGCAT
    TATCGGTCCC TGGGAGGCGA AGGGAACTTT AACTGGAGGT TCATTTTCCC CTTTGACTAC
    CTGCTGGCTG AGCAAGTCTG CACCGTCTCC AAGAAGGATG CTTTCTGGAA GCTGGACAAG
    ACCGAGAGCA AAATCCCAGC ACGAGTGGTA TTCCAGATCT GGGACAATGA CAAGTTCTCC
    TTTGATGATT TCCTGGGCTC CTTGCAGCTG GATCTCAACC GCATGCCCAA GCCTGCCAAG
    ACAGCCGAGA AGTGCTCCCT GGACCAGCTG GATGACACTT TCCACCCAGA GTGGTTTGTG
    TCCCTTTTTG AACAGAAAAC TGTGAAGGGC TGGTGGCCCT GTGTGGCGGA AGAGGGTGAG
    AAGAAGAAGC TGGCGGGCAA GCTGGAGATG ACCTTGGAGA TTGTGGCGGA GAGTGAGCAT
    GAGGAGCGGC CCGCTGGCCA GGGCCGGGAT GAGCCCAACA TGAACCCCAA GCTCGAGGAC
    CCAAGGCGCC CTGACACCTC CTTCCTCTGG TTCACCTCCC CGTACAAGAC TATGAAGTTC
    ATCCTGTGGC GGCGTTTCCG GTGTGCCATC ATCCTCTTCA TCATCCTCTT CATCCTACTG
    CTGTTCTTGG GCATCTTCGT TTACGCCTTC CCGAACTATG CTGCCATGAA GCTGGTGAAG
    CCCTTCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb49787
    Clone ID Related Accession (Same CDS sequence) XM_023618919.1
    Accession Version XM_023618919.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product dysferlin isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009158.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTGTGCT GCCTGCTAGT GAAGGCCAGC AACCTTCCCA CTGTGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAA
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG GGTCCCCCTG
    GACCAGAGCT CTGAGCTTCA CGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
    TTCCTGGGAG AAGCCAAGGT CCCACTCCGG GAGGTCCTTG CCACCCCCAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACGCCTCT CCCTGGAGCT GTGCCCCTGT TCCCACCCTC TGTGCCGCTG
    GAACCCTCCC CGACTCTGCC CGATGTGGAT ATGCTGGCTG ATACAGGAGG AGAGGAGGAC
    ACCGAGGACC AGGGGCTCAC GGGAGATGAG GCAGAGCCAT TCTTGGATCA GAACGGAGCC
    CCAGGCCCCG GGGCTCCCAC CACCCTGAAG AAGCCACCTT CCCATCCTCC CCCCTACCAT
    CCTGGGGGGA AAAGGAAGAG AAGCACGCCT GCGCCCAGAA AGCTGCTTTC GGATAAACCA
    CAGGACTTCC AGATCAGGGT CCAGGTGATC GAGGGGCGCC AGCTGCCAGG GGTGAACATC
    AAGCCTGTGG TCAAGGTCAC CGCGGCCAGG CAGACCAAGC GGACTCGGAT TCACAGGGGA
    AACAGCCCGC TCTTCAACGA GACTCTCTTC TTCAACGTGT TTGACTCTCC CTCGGAGCTG
    TTTGACGAGG CCGTCTTTAT CACGGTGGTA GACTCCTGTT CGCTCCGGAC AGATGCCCTC
    ATCGGGGAGT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA CGCCTATCTC
    AGGAAGTGGC TGCTGCTCTC GGACCCTGAC GATTTCTCTG CTGGGCCCAA AGGCTACCTG
    AAAGCAAGCC TTTGTGTGCT GGGGCCTGGA GACGAAGCTC CGCTGGAGAG AAAGGACCCC
    TCTGAAGACA AGGAGGACAT TGAAAGCAAT CTGCTCAGGC CAACTGGCAT GGCCCTTCGA
    GGAGCGCACT TCTGTCTGAA GGTCTTCAGG GCTGAGGACT TACCACAGAT GGACGATGCC
    GTGGTGGACA GCGTGAAGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CCTGGTGGAT
    CCCTTCGTCG AGGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGATG
    GCCAACCCTC AGTGGAACCA GAGCATCACG CTGCCTGTCA TGTTTCCCTC CATGTCTGAA
    AAAATGAGGA TTCGTGTCAT AGACTGGGAC CGCCTCACCC ACAATGACAT CGTGGCCACC
    ACCTACCTGA ATATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGCAGT GGACGACCAC
    CTGGGCTTCC TCCCCACCTT CGGGCCCTGC TATGTCAACC TCTACGGCAG CCCCAGGGAG
    TTCACTGGCT TCCCAGACCC CTATGCAGAG CTCAACACGG GCAAGGGAGA AGGCGTGGCC
    TACCGAGGCC GGCTTCTGGT CTCCCTGGAG ACCAAACTGG TGGAGCAAGG CGAGCAGAAG
    GTGGAGGATC TCGCTGCAGA TGACATCCTT CGGGTGGAGA AGTACCTCCG GAGGCGCAAG
    TACTCCCTCT TTGCTGCCTT CTACTCGGCC TGCATGCTGC AGGATGTCGA CGATGCCATC
    CAGTTCGAGG TCAGCATCGG AAACTACGGG AACAAGTTTG ACACGACCTG CCTGCCGCTG
    GCCTCCACCA CCCAGTACAG CCGGGCGGTC TTTGACGGGT GCCACTACTA CTACTTACCC
    TGGGGCAACG TGAAGCCTGT GGTGGTGCTG TCATCCTACT GGGAAGACAT CAGCCACAGA
    GTCGACACTC AGAACCAGCT GCTCCGGATC GCCGACCGGC TGGAAGCCGG CCTGGAGCAG
    GTCCACCTGG CCCTGAAGGC GCGGTGCTCC ACCGAGGATG TGGACTCCCT GGTGGCTCAG
    CTGATGGATG AGCTCATTGC AGACTGCAGC CAGCCCCTGT GCAACGTCCA AGAGACGCCC
    TCTGCCACCC ACCTGGACCA GTACCTGTAC CGGCTGCGCA CCCGTCACCT GAGCCAGATC
    AGTGAGGCGG CCCTGGCCTT GAAGCTCGGC CACAGTGAGC TCCCCGCCGC CCTTGAGCAG
    GCTGAGGACT GGCTCCTGCG TCTCCGTGCC CTGGCGGAGG AGCCCCAGAA CAGCCTGCCT
    GACGTGGTCA TCTGGATGCT GCAGGGAGAC AAGCGGGTGG CGTACCAGCG GGTGCCCGCC
    CACGAGGTCC TCTTCTCCCG GCGGGGCGCC AACTACTGTG GCAAGAATTG TGGGAAGCTG
    CAGACGATCT TTCTGAAGTA TCCAATGGAA GGGGTGCCGG GAGCCCGGAT GCCGGTGCAG
    ATACGGGTGA AGCTCTGGTT CGGGCTCTCT GTGGATGAGA AGGAGTTCAA TCAGTTTGCT
    GAGGGCAAGC TCTCTGTCTT CGCTGAAACC TACGAGAACC AGACCAAGCT GGCCCTAGTC
    GGGAACTGGG GCACAACGGG CCTCACCTAC CCCAAGTTTT CTGACGTCAC AGGCAAGATC
    AAGCTACCCA AGGACAGCTT CCGCCCCTCG GCCGGCTGGG CCTGGGCTGG AGAGTGGTTC
    GTGTGTCCGG AGAAGACCCT ACTCCACGAC ACCGATGCGG GCCACCTGAG CTTTGTGGAG
    GAGGTGTTTG AGAACCAGAC CCGGCTGCCC GGAGGCCAGT GGATCTACAT GAGTGACAAC
    TACACAGATG TGAACGGGGA GAAGGTGCTT CCCAAGGATG ACATCGAGTG CCCACTGGGC
    TGGAAGTGGG AAGATGAAGA ATGGTCCACA GACCTCAATC GGGCCGTTGA CGAGCAAGGC
    TGGGAGTACA GCATCACCAT CCCTCCGGAA CGGAAGCCAA GGCACTGGGT CCCTGCCGAG
    AAGATGTACT ACACACACCG TCGGCGGCGC TGGGTCCGCC TGCGCAGGAG GGACCTCAGC
    CAGATGGAGG CGCTGAAGAG GCACAGGCAG GCGGAGGCGG AGGGCGAGGG CTGGGAGTAC
    GCCTCGCTCT TCGGCTGGAA GTTCCACCTG GAGTACCGCA AGACCGATGC CTTCCGCCGC
    CGCCGCTGGC GCCGCCGCAT GGAGCCACTG GAGAAGACAG GGCCTGCAGC TGTGTTTGCC
    CTCGAGGGGG CCCTGGGCGG CGTGGTGGAC GACAGGAGTG AAGATTCGGT GTCCGTCTCC
    ACCTTGAGCT TCGGCGTGAA CAGACCCACG ATTTCCTGCA TCTTCGACTA CGGGAACCGC
    TACCATCTAC GCTGCTACAT GTACCAGGCC CGGGACCTGC CCGCCATGGA CAAGGACTCT
    TTTTCTGATC CCTACGCAAT CGTCTCCTTC CTGCACCAGA GCCAGAAGAC AGTGGTGGTG
    AAGAACACCC TGAACCCCAC CTGGGACCAG ACACTCATCT TCTATGAGAT CGAGATCTTT
    GGTGAGCCGC CCAGCATCGC CGAGCAGCCG CCCAGCATCG TGGTGGAGCT ATACGACCAT
    GACACCTACG GTGCAGATGA GTTTATGGGA CGCTGCATCT GTCAGCCGAC TCTGGAACGG
    ATGCCCCGGC TGACCTGGTT CCCACTGACT AGGGGCAGCC AGCCGGCGGG CGAGCTGCTG
    GCCTCTTTTG AGCTCATCCA GAGAGAGAAG CCGGCCATCT ACCACATTCC TGGTTTTGAG
    GTGCAGGATA CATCAGGGAT CCTGGAAGAG TCGGAGGACA CAGACCTGCC CTACCCGCCA
    CCCCAGAGAG AGGCCAACAT CTACATGGTT CCCCAGAACA TCAAGCCTGC TCTCCAGCGG
    ACGGCCATCG AGATCCTGGC ATGGGGCCTG CGGAACATGA AGAGTTACCA CCTGGCCAGT
    GTCTCCTCCC CCAGCCTCGT GGTGGAGTGC GGGGGCCAGA CGGTGCAGTC CTGTGTCATC
    AGGAACCTCC AGAAGAACCC CAACTTCGAC GTCTGCACCC TCTTCATGGA AGTGATGCTG
    CCCAGGGAGG AGCTCTACTG TCCCCCCATC GTGGTCAAGG TCATCGATAA CTGCCAGTTT
    GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCTGCTCCC TGGAGAGCTT CCTGTGTGAC
    CCCTACTCAG AGGAGAGTCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG CTTACTCAGT
    CCTGGGGAAG ATGTGCTCAT TGACATTGAT GACAAGGAGC CCCTCATCCC TATGCAGGAG
    GAGGAGTTCA TCGATTGGTG GAGCAAATTC TTTGCCTCGA TAGGGGAGAG GGAAAAGTGC
    GGGTCCTACT TGGAGAAGGA CTTTGACACT CTAAAGGTCT ATGACACACA ACTGGAGAAT
    GTGGAGGCCT TTGAGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG
    ACTCAGGAGG AGATGGAAGA CCCATCTGTC GTTGGGGAAT TTAAGGGCCT CTTCAAAATT
    TATCCCCTCC CAGAAGACCC GGCCATCCCC ATGCCCCCAA GACAGTTCCA CCAGCTAGCT
    GCCCAGGGAC CCCAGGAGTG CCTGGTCCGC ATCTACATCA TCCGGGCATT CGGCCTGCAG
    CCCAAGGACC CCAATGGGAA GTGTGACCCT TACATCAAGA TCTCCATAGG GAAGAAATCC
    GTGAGCGACC AGGATAACTA CATCCCCTGC ACTCTGGAGC CTGTGTTTGG AAAGATGTTC
    GAACTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACTCTCTA TGACTATGAC
    CTCCTCTCCA AGGACGAGAT GATCGGGGAG ACGGTCATCG ACCTGGAGAA TAGGCTGCTG
    TCCAAGTTTG GAGCTCGCTG CGGACTCCCA CAGACCTACT GTGTCTCTGG ACCGAACCAG
    TGGAGGGACC AGCTCCGCCC CTCCCAGCTT CTCCACCTGT TCTGCCAGCA GCAGAGAGTC
    AAGGCCCCCG TGTACCAGAC CGACCGTGTG GTGTTTGGGA ATAAAGAATA CACGATTGAA
    GAAATAGAGG CTGGGAGGGT CCTGAATCCA CACCTGGGCC CAGTGGAAGA GCGCCTGGCC
    TTGCACGTCC TCCAGCAGCA GGGCTTGGTG CCCGAGCATG TGGAGTCCCG GCCCCTCTAT
    AGCCCCCTGC AGCCGGACAT TGAGCAGGGG AAGCTGCAGA TGTGGATCGA CCTGTTTCCA
    AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC AACATCACTC CACGGAGAGC CAGAAGGTTT
    TTCCTGCGTT GCATTATCTG GAACACCAAG GATGTGATCC TGGACGACCT CAGCATCACA
    GGAGAGAAGA TGAGCGACAT TTATGTGAAA GGTTGGATGG TTGGCTTTGA AGAACACAAG
    CAAAAGACAG ATGTGCATTA TCGGTCCCTG GGAGGCGAAG GGAACTTTAA CTGGAGGTTC
    ATTTTCCCCT TTGACTACCT GCTGGCTGAG CAAGTCTGCA CCGTCTCCAA GAAGGATGCT
    TTCTGGAAGC TGGACAAGAC CGAGAGCAAA ATCCCAGCAC GAGTGGTATT CCAGATCTGG
    GACAATGACA AGTTCTCCTT TGATGATTTC CTGGGCTCCT TGCAGCTGGA TCTCAACCGC
    ATGCCCAAGC CTGCCAAGAC AGCCGAGAAG TGCTCCCTGG ACCAGCTGGA TGACACTTTC
    CACCCAGAGT GGTTTGTGTC CCTTTTTGAA CAGAAAACTG TGAAGGGCTG GTGGCCCTGT
    GTGGCGGAAG AGGGTGAGAA GAAGAAGCTG GCGGGCAAGC TGGAGATGAC CTTGGAGATT
    GTGGCGGAGA GTGAGCATGA GGAGCGGCCC GCTGGCCAGG GCCGGGATGA GCCCAACATG
    AACCCCAAGC TCGAGGACCC AAGGCGCCCT GACACCTCCT TCCTCTGGTT CACCTCCCCG
    TACAAGACTA TGAAGTTCAT CCTGTGGCGG CGTTTCCGGT GTGCCATCAT CCTCTTCATC
    ATCCTCTTCA TCCTACTGCT GTTCTTGGGC ATCTTCGTTT ACGCCTTCCC GAACTATGCT
    GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023474687.1
    CDS210..6419
    Translation

    Target ORF information:

    RefSeq Version XM_023618919.1
    Organism Equus caballus(horse)
    Definition Equus caballus dysferlin (DYSF), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023618919.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTGTGCT GCCTGCTAGT GAAGGCCAGC AACCTTCCCA CTGTGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAA
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG GGTCCCCCTG
    GACCAGAGCT CTGAGCTTCA CGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
    TTCCTGGGAG AAGCCAAGGT CCCACTCCGG GAGGTCCTTG CCACCCCCAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACGCCTCT CCCTGGAGCT GTGCCCCTGT TCCCACCCTC TGTGCCGCTG
    GAACCCTCCC CGACTCTGCC CGATGTGGAT ATGCTGGCTG ATACAGGAGG AGAGGAGGAC
    ACCGAGGACC AGGGGCTCAC GGGAGATGAG GCAGAGCCAT TCTTGGATCA GAACGGAGCC
    CCAGGCCCCG GGGCTCCCAC CACCCTGAAG AAGCCACCTT CCCATCCTCC CCCCTACCAT
    CCTGGGGGGA AAAGGAAGAG AAGCACGCCT GCGCCCAGAA AGCTGCTTTC GGATAAACCA
    CAGGACTTCC AGATCAGGGT CCAGGTGATC GAGGGGCGCC AGCTGCCAGG GGTGAACATC
    AAGCCTGTGG TCAAGGTCAC CGCGGCCAGG CAGACCAAGC GGACTCGGAT TCACAGGGGA
    AACAGCCCGC TCTTCAACGA GACTCTCTTC TTCAACGTGT TTGACTCTCC CTCGGAGCTG
    TTTGACGAGG CCGTCTTTAT CACGGTGGTA GACTCCTGTT CGCTCCGGAC AGATGCCCTC
    ATCGGGGAGT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA CGCCTATCTC
    AGGAAGTGGC TGCTGCTCTC GGACCCTGAC GATTTCTCTG CTGGGCCCAA AGGCTACCTG
    AAAGCAAGCC TTTGTGTGCT GGGGCCTGGA GACGAAGCTC CGCTGGAGAG AAAGGACCCC
    TCTGAAGACA AGGAGGACAT TGAAAGCAAT CTGCTCAGGC CAACTGGCAT GGCCCTTCGA
    GGAGCGCACT TCTGTCTGAA GGTCTTCAGG GCTGAGGACT TACCACAGAT GGACGATGCC
    GTGGTGGACA GCGTGAAGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CCTGGTGGAT
    CCCTTCGTCG AGGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGATG
    GCCAACCCTC AGTGGAACCA GAGCATCACG CTGCCTGTCA TGTTTCCCTC CATGTCTGAA
    AAAATGAGGA TTCGTGTCAT AGACTGGGAC CGCCTCACCC ACAATGACAT CGTGGCCACC
    ACCTACCTGA ATATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGCAGT GGACGACCAC
    CTGGGCTTCC TCCCCACCTT CGGGCCCTGC TATGTCAACC TCTACGGCAG CCCCAGGGAG
    TTCACTGGCT TCCCAGACCC CTATGCAGAG CTCAACACGG GCAAGGGAGA AGGCGTGGCC
    TACCGAGGCC GGCTTCTGGT CTCCCTGGAG ACCAAACTGG TGGAGCAAGG CGAGCAGAAG
    GTGGAGGATC TCGCTGCAGA TGACATCCTT CGGGTGGAGA AGTACCTCCG GAGGCGCAAG
    TACTCCCTCT TTGCTGCCTT CTACTCGGCC TGCATGCTGC AGGATGTCGA CGATGCCATC
    CAGTTCGAGG TCAGCATCGG AAACTACGGG AACAAGTTTG ACACGACCTG CCTGCCGCTG
    GCCTCCACCA CCCAGTACAG CCGGGCGGTC TTTGACGGGT GCCACTACTA CTACTTACCC
    TGGGGCAACG TGAAGCCTGT GGTGGTGCTG TCATCCTACT GGGAAGACAT CAGCCACAGA
    GTCGACACTC AGAACCAGCT GCTCCGGATC GCCGACCGGC TGGAAGCCGG CCTGGAGCAG
    GTCCACCTGG CCCTGAAGGC GCGGTGCTCC ACCGAGGATG TGGACTCCCT GGTGGCTCAG
    CTGATGGATG AGCTCATTGC AGACTGCAGC CAGCCCCTGT GCAACGTCCA AGAGACGCCC
    TCTGCCACCC ACCTGGACCA GTACCTGTAC CGGCTGCGCA CCCGTCACCT GAGCCAGATC
    AGTGAGGCGG CCCTGGCCTT GAAGCTCGGC CACAGTGAGC TCCCCGCCGC CCTTGAGCAG
    GCTGAGGACT GGCTCCTGCG TCTCCGTGCC CTGGCGGAGG AGCCCCAGAA CAGCCTGCCT
    GACGTGGTCA TCTGGATGCT GCAGGGAGAC AAGCGGGTGG CGTACCAGCG GGTGCCCGCC
    CACGAGGTCC TCTTCTCCCG GCGGGGCGCC AACTACTGTG GCAAGAATTG TGGGAAGCTG
    CAGACGATCT TTCTGAAGTA TCCAATGGAA GGGGTGCCGG GAGCCCGGAT GCCGGTGCAG
    ATACGGGTGA AGCTCTGGTT CGGGCTCTCT GTGGATGAGA AGGAGTTCAA TCAGTTTGCT
    GAGGGCAAGC TCTCTGTCTT CGCTGAAACC TACGAGAACC AGACCAAGCT GGCCCTAGTC
    GGGAACTGGG GCACAACGGG CCTCACCTAC CCCAAGTTTT CTGACGTCAC AGGCAAGATC
    AAGCTACCCA AGGACAGCTT CCGCCCCTCG GCCGGCTGGG CCTGGGCTGG AGAGTGGTTC
    GTGTGTCCGG AGAAGACCCT ACTCCACGAC ACCGATGCGG GCCACCTGAG CTTTGTGGAG
    GAGGTGTTTG AGAACCAGAC CCGGCTGCCC GGAGGCCAGT GGATCTACAT GAGTGACAAC
    TACACAGATG TGAACGGGGA GAAGGTGCTT CCCAAGGATG ACATCGAGTG CCCACTGGGC
    TGGAAGTGGG AAGATGAAGA ATGGTCCACA GACCTCAATC GGGCCGTTGA CGAGCAAGGC
    TGGGAGTACA GCATCACCAT CCCTCCGGAA CGGAAGCCAA GGCACTGGGT CCCTGCCGAG
    AAGATGTACT ACACACACCG TCGGCGGCGC TGGGTCCGCC TGCGCAGGAG GGACCTCAGC
    CAGATGGAGG CGCTGAAGAG GCACAGGCAG GCGGAGGCGG AGGGCGAGGG CTGGGAGTAC
    GCCTCGCTCT TCGGCTGGAA GTTCCACCTG GAGTACCGCA AGACCGATGC CTTCCGCCGC
    CGCCGCTGGC GCCGCCGCAT GGAGCCACTG GAGAAGACAG GGCCTGCAGC TGTGTTTGCC
    CTCGAGGGGG CCCTGGGCGG CGTGGTGGAC GACAGGAGTG AAGATTCGGT GTCCGTCTCC
    ACCTTGAGCT TCGGCGTGAA CAGACCCACG ATTTCCTGCA TCTTCGACTA CGGGAACCGC
    TACCATCTAC GCTGCTACAT GTACCAGGCC CGGGACCTGC CCGCCATGGA CAAGGACTCT
    TTTTCTGATC CCTACGCAAT CGTCTCCTTC CTGCACCAGA GCCAGAAGAC AGTGGTGGTG
    AAGAACACCC TGAACCCCAC CTGGGACCAG ACACTCATCT TCTATGAGAT CGAGATCTTT
    GGTGAGCCGC CCAGCATCGC CGAGCAGCCG CCCAGCATCG TGGTGGAGCT ATACGACCAT
    GACACCTACG GTGCAGATGA GTTTATGGGA CGCTGCATCT GTCAGCCGAC TCTGGAACGG
    ATGCCCCGGC TGACCTGGTT CCCACTGACT AGGGGCAGCC AGCCGGCGGG CGAGCTGCTG
    GCCTCTTTTG AGCTCATCCA GAGAGAGAAG CCGGCCATCT ACCACATTCC TGGTTTTGAG
    GTGCAGGATA CATCAGGGAT CCTGGAAGAG TCGGAGGACA CAGACCTGCC CTACCCGCCA
    CCCCAGAGAG AGGCCAACAT CTACATGGTT CCCCAGAACA TCAAGCCTGC TCTCCAGCGG
    ACGGCCATCG AGATCCTGGC ATGGGGCCTG CGGAACATGA AGAGTTACCA CCTGGCCAGT
    GTCTCCTCCC CCAGCCTCGT GGTGGAGTGC GGGGGCCAGA CGGTGCAGTC CTGTGTCATC
    AGGAACCTCC AGAAGAACCC CAACTTCGAC GTCTGCACCC TCTTCATGGA AGTGATGCTG
    CCCAGGGAGG AGCTCTACTG TCCCCCCATC GTGGTCAAGG TCATCGATAA CTGCCAGTTT
    GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCTGCTCCC TGGAGAGCTT CCTGTGTGAC
    CCCTACTCAG AGGAGAGTCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG CTTACTCAGT
    CCTGGGGAAG ATGTGCTCAT TGACATTGAT GACAAGGAGC CCCTCATCCC TATGCAGGAG
    GAGGAGTTCA TCGATTGGTG GAGCAAATTC TTTGCCTCGA TAGGGGAGAG GGAAAAGTGC
    GGGTCCTACT TGGAGAAGGA CTTTGACACT CTAAAGGTCT ATGACACACA ACTGGAGAAT
    GTGGAGGCCT TTGAGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG
    ACTCAGGAGG AGATGGAAGA CCCATCTGTC GTTGGGGAAT TTAAGGGCCT CTTCAAAATT
    TATCCCCTCC CAGAAGACCC GGCCATCCCC ATGCCCCCAA GACAGTTCCA CCAGCTAGCT
    GCCCAGGGAC CCCAGGAGTG CCTGGTCCGC ATCTACATCA TCCGGGCATT CGGCCTGCAG
    CCCAAGGACC CCAATGGGAA GTGTGACCCT TACATCAAGA TCTCCATAGG GAAGAAATCC
    GTGAGCGACC AGGATAACTA CATCCCCTGC ACTCTGGAGC CTGTGTTTGG AAAGATGTTC
    GAACTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACTCTCTA TGACTATGAC
    CTCCTCTCCA AGGACGAGAT GATCGGGGAG ACGGTCATCG ACCTGGAGAA TAGGCTGCTG
    TCCAAGTTTG GAGCTCGCTG CGGACTCCCA CAGACCTACT GTGTCTCTGG ACCGAACCAG
    TGGAGGGACC AGCTCCGCCC CTCCCAGCTT CTCCACCTGT TCTGCCAGCA GCAGAGAGTC
    AAGGCCCCCG TGTACCAGAC CGACCGTGTG GTGTTTGGGA ATAAAGAATA CACGATTGAA
    GAAATAGAGG CTGGGAGGGT CCTGAATCCA CACCTGGGCC CAGTGGAAGA GCGCCTGGCC
    TTGCACGTCC TCCAGCAGCA GGGCTTGGTG CCCGAGCATG TGGAGTCCCG GCCCCTCTAT
    AGCCCCCTGC AGCCGGACAT TGAGCAGGGG AAGCTGCAGA TGTGGATCGA CCTGTTTCCA
    AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC AACATCACTC CACGGAGAGC CAGAAGGTTT
    TTCCTGCGTT GCATTATCTG GAACACCAAG GATGTGATCC TGGACGACCT CAGCATCACA
    GGAGAGAAGA TGAGCGACAT TTATGTGAAA GGTTGGATGG TTGGCTTTGA AGAACACAAG
    CAAAAGACAG ATGTGCATTA TCGGTCCCTG GGAGGCGAAG GGAACTTTAA CTGGAGGTTC
    ATTTTCCCCT TTGACTACCT GCTGGCTGAG CAAGTCTGCA CCGTCTCCAA GAAGGATGCT
    TTCTGGAAGC TGGACAAGAC CGAGAGCAAA ATCCCAGCAC GAGTGGTATT CCAGATCTGG
    GACAATGACA AGTTCTCCTT TGATGATTTC CTGGGCTCCT TGCAGCTGGA TCTCAACCGC
    ATGCCCAAGC CTGCCAAGAC AGCCGAGAAG TGCTCCCTGG ACCAGCTGGA TGACACTTTC
    CACCCAGAGT GGTTTGTGTC CCTTTTTGAA CAGAAAACTG TGAAGGGCTG GTGGCCCTGT
    GTGGCGGAAG AGGGTGAGAA GAAGAAGCTG GCGGGCAAGC TGGAGATGAC CTTGGAGATT
    GTGGCGGAGA GTGAGCATGA GGAGCGGCCC GCTGGCCAGG GCCGGGATGA GCCCAACATG
    AACCCCAAGC TCGAGGACCC AAGGCGCCCT GACACCTCCT TCCTCTGGTT CACCTCCCCG
    TACAAGACTA TGAAGTTCAT CCTGTGGCGG CGTTTCCGGT GTGCCATCAT CCTCTTCATC
    ATCCTCTTCA TCCTACTGCT GTTCTTGGGC ATCTTCGTTT ACGCCTTCCC GAACTATGCT
    GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb49788
    Clone ID Related Accession (Same CDS sequence) XM_023618920.1
    Accession Version XM_023618920.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6447bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product dysferlin isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009158.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGAGGG TCTTTTTCCT TTACGCCGAG AACGTCCACA CCCCGGACAC CGACATCAGC 
    GATGCCTACT GCTCCGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAAAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGGGT CCCCCTGGAC
    CAGAGCTCTG AGCTTCACGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
    CTGGGAGAAG CCAAGGTCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CGCCTCTCCC TGGAGCTGTG CCCCTGTTCC CACCCTCTGT GCCGCTGGAA
    CCCTCCCCGA CTCTGCCCGA TGTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCCGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAATGGCAG
    GCCCCCACGG ATACAGGAGG AGAGGAGGAC ACCGAGGACC AGGGGCTCAC GGGAGATGAG
    GCAGAGCCAT TCTTGGATCA GAACGGAGCC CCAGGCCCCG GGGCTCCCAC CACCCTGAAG
    AAGCCACCTT CCCATCCTCC CCCCTACCAT CCTGGGGGGA AAAGGAAGAG AAGCACGCCT
    GCGCCCAGAA AGCTGCTTTC GGATAAACCA CAGGACTTCC AGATCAGGGT CCAGGTGATC
    GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC CGCGGCCAGG
    CAGACCAAGC GGACTCGGAT TCACAGGGGA AACAGCCCGC TCTTCAACGA GACTCTCTTC
    TTCAACGTGT TTGACTCTCC CTCGGAGCTG TTTGACGAGG CCGTCTTTAT CACGGTGGTA
    GACTCCTGTT CGCTCCGGAC AGATGCCCTC ATCGGGGAGT TCCGGATGGA TGTGGGTACC
    ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTGCTCTC GGACCCTGAC
    GATTTCTCTG CTGGGCCCAA AGGCTACCTG AAAGCAAGCC TTTGTGTGCT GGGGCCTGGA
    GACGAAGCTC CGCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAGCAAT
    CTGCTCAGGC CAACTGGCAT GGCCCTTCGA GGAGCGCACT TCTGTCTGAA GGTCTTCAGG
    GCTGAGGACT TACCACAGAT GGACGATGCC GTGGTGGACA GCGTGAAGCA GATCTTCGGC
    TTTGACAGTA ACAAGAAGAA CCTGGTGGAT CCCTTCGTCG AGGTCAGCTT TGCGGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGATG GCCAACCCTC AGTGGAACCA GAGCATCACG
    CTGCCTGTCA TGTTTCCCTC CATGTCTGAA AAAATGAGGA TTCGTGTCAT AGACTGGGAC
    CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA ATATGTCGAA AATCTCTGCC
    CCTGGAGGAG AAATAGCAGA GGAGCCTGCA GGTGTGGTCA AGCCTCCTCC AGCCACGGAC
    CTGGACGACC ACCTGGGCTT CCTCCCCACC TTCGGGCCCT GCTATGTCAA CCTCTACGGC
    AGCCCCAGGG AGTTCACTGG CTTCCCAGAC CCCTATGCAG AGCTCAACAC GGGCAAGGGA
    GAAGGCGTGG CCTACCGAGG CCGGCTTCTG GTCTCCCTGG AGACCAAACT GGTGGAGCAA
    GGCGAGCAGA AGGTGGAGGA TCTCGCTGCA GATGACATCC TTCGGGTGGA GAAGTACCTC
    CGGAGGCGCA AGTACTCCCT CTTTGCTGCC TTCTACTCGG CCTGCATGCT GCAGGATGTC
    GACGATGCCA TCCAGTTCGA GGTCAGCATC GGAAACTACG GGAACAAGTT TGACACGACC
    TGCCTGCCGC TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGACGG GTGCCACTAC
    TACTACTTAC CCTGGGGCAA CGTGAAGCCT GTGGTGGTGC TGTCATCCTA CTGGGAAGAC
    ATCAGCCACA GAGTCGACAC TCAGAACCAG CTGCTCCGGA TCGCCGACCG GCTGGAAGCC
    GGCCTGGAGC AGGTCCACCT GGCCCTGAAG GCGCGGTGCT CCACCGAGGA TGTGGACTCC
    CTGGTGGCTC AGCTGATGGA TGAGCTCATT GCAGACTGCA GCCAGCCCCT GTGCAACGTC
    CAAGAGACGC CCTCTGCCAC CCACCTGGAC CAGTACCTGT ACCGGCTGCG CACCCGTCAC
    CTGAGCCAGA TCAGTGAGGC GGCCCTGGCC TTGAAGCTCG GCCACAGTGA GCTCCCCGCC
    GCCCTTGAGC AGGCTGAGGA CTGGCTCCTG CGTCTCCGTG CCCTGGCGGA GGAGCCCCAG
    AACAGCCTGC CTGACGTGGT CATCTGGATG CTGCAGGGAG ACAAGCGGGT GGCGTACCAG
    CGGGTGCCCG CCCACGAGGT CCTCTTCTCC CGGCGGGGCG CCAACTACTG TGGCAAGAAT
    TGTGGGAAGC TGCAGACGAT CTTTCTGAAG TATCCAATGG AAGGGGTGCC GGGAGCCCGG
    ATGCCGGTGC AGATACGGGT GAAGCTCTGG TTCGGGCTCT CTGTGGATGA GAAGGAGTTC
    AATCAGTTTG CTGAGGGCAA GCTCTCTGTC TTCGCTGAAA CCTACGAGAA CCAGACCAAG
    CTGGCCCTAG TCGGGAACTG GGGCACAACG GGCCTCACCT ACCCCAAGTT TTCTGACGTC
    ACAGGCAAGA TCAAGCTACC CAAGGACAGC TTCCGCCCCT CGGCCGGCTG GGCCTGGGCT
    GGAGAGTGGT TCGTGTGTCC GGAGAAGACC CTACTCCACG ACACCGATGC GGGCCACCTG
    AGCTTTGTGG AGGAGGTGTT TGAGAACCAG ACCCGGCTGC CCGGAGGCCA GTGGATCTAC
    ATGAGTGACA ACTACACAGA TGTGAACGGG GAGAAGGTGC TTCCCAAGGA TGACATCGAG
    TGCCCACTGG GCTGGAAGTG GGAAGATGAA GAATGGTCCA CAGACCTCAA TCGGGCCGTT
    GACGAGCAAG GCTGGGAGTA CAGCATCACC ATCCCTCCGG AACGGAAGCC AAGGCACTGG
    GTCCCTGCCG AGAAGATGTA CTACACACAC CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG
    AGGGACCTCA GCCAGATGGA GGCGCTGAAG AGGCACAGGC AGGCGGAGGC GGAGGGCGAG
    GGCTGGGAGT ACGCCTCGCT CTTCGGCTGG AAGTTCCACC TGGAGTACCG CAAGACCGAT
    GCCTTCCGCC GCCGCCGCTG GCGCCGCCGC ATGGAGCCAC TGGAGAAGAC AGGGCCTGCA
    GCTGTGTTTG CCCTCGAGGG GGCCCTGGGC GGCGTGGTGG ACGACAGGAG TGAAGATTCG
    GTGTCCGTCT CCACCTTGAG CTTCGGCGTG AACAGACCCA CGATTTCCTG CATCTTCGAC
    TACGGGAACC GCTACCATCT ACGCTGCTAC ATGTACCAGG CCCGGGACCT GCCCGCCATG
    GACAAGGACT CTTTTTCTGA TCCCTACGCA ATCGTCTCCT TCCTGCACCA GAGCCAGAAG
    ACAGTGGTGG TGAAGAACAC CCTGAACCCC ACCTGGGACC AGACACTCAT CTTCTATGAG
    ATCGAGATCT TTGGTGAGCC GCCCAGCATC GCCGAGCAGC CGCCCAGCAT CGTGGTGGAG
    CTATACGACC ATGACACCTA CGGTGCAGAT GAGTTTATGG GACGCTGCAT CTGTCAGCCG
    ACTCTGGAAC GGATGCCCCG GCTGACCTGG TTCCCACTGA CTAGGGGCAG CCAGCCGGCG
    GGCGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCGGCCAT CTACCACATT
    CCTGGTTTTG AGGTGCAGGA TACATCAGGG ATCCTGGAAG AGCTGTGTGC ACCTGCCTTC
    TTCCTCCAGG GCCTCCTCCA AACGTCGGAG GACACAGACC TGCCCTACCC GCCACCCCAG
    AGAGAGGCCA ACATCTACAT GGTTCCCCAG AACATCAAGC CTGCTCTCCA GCGGACGGCC
    ATCGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCACCTGGC CAGTGTCTCC
    TCCCCCAGCC TCGTGGTGGA GTGCGGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC
    CTCCAGAAGA ACCCCAACTT CGACGTCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG
    GAGGAGCTCT ACTGTCCCCC CATCGTGGTC AAGGTCATCG ATAACTGCCA GTTTGGCCGC
    CGGCCTGTGG TGGGCCAGTG TACCATCTGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC
    TCAGAGGAGA GTCCGTCCCC ACAGGGCGGC CCAGATGATG TGAGCTTACT CAGTCCTGGG
    GAAGATGTGC TCATTGACAT TGATGACAAG GAGCCCCTCA TCCCTATGCA GCTTGCAGAT
    GGTCTGTCGG GCTCGGCCCC CACTAACATG GCATCTTCTC CGTCCAGTCC TCATGAGGAG
    GAGTTCATCG ATTGGTGGAG CAAATTCTTT GCCTCGATAG GGGAGAGGGA AAAGTGCGGG
    TCCTACTTGG AGAAGGACTT TGACACTCTA AAGGTCTATG ACACACAACT GGAGAATGTG
    GAGGCCTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTCTACCG GGGCAAGACT
    CAGGAGGAGA TGGAAGACCC ATCTGTCGTT GGGGAATTTA AGGGCCTCTT CAAAATTTAT
    CCCCTCCCAG AAGACCCGGC CATCCCCATG CCCCCAAGAC AGTTCCACCA GCTAGCTGCC
    CAGGGACCCC AGGAGTGCCT GGTCCGCATC TACATCATCC GGGCATTCGG CCTGCAGCCC
    AAGGACCCCA ATGGGAAGTG TGACCCTTAC ATCAAGATCT CCATAGGGAA GAAATCCGTG
    AGCGACCAGG ATAACTACAT CCCCTGCACT CTGGAGCCTG TGTTTGGAAA GATGTTCGAA
    CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGATCA CTCTCTATGA CTATGACCTC
    CTCTCCAAGG ACGAGATGAT CGGGGAGACG GTCATCGACC TGGAGAATAG GCTGCTGTCC
    AAGTTTGGAG CTCGCTGCGG ACTCCCACAG ACCTACTGTG TCTCTGGACC GAACCAGTGG
    AGGGACCAGC TCCGCCCCTC CCAGCTTCTC CACCTGTTCT GCCAGCAGCA GAGAGTCAAG
    GCCCCCGTGT ACCAGACCGA CCGTGTGGTG TTTGGGAATA AAGAATACAC GATTGAAGAA
    ATAGAGGCTG GGAGGGTCCT GAATCCACAC CTGGGCCCAG TGGAAGAGCG CCTGGCCTTG
    CACGTCCTCC AGCAGCAGGG CTTGGTGCCC GAGCATGTGG AGTCCCGGCC CCTCTATAGC
    CCCCTGCAGC CGGACATTGA GCAGGGGAAG CTGCAGATGT GGATCGACCT GTTTCCAAAG
    GCCCTGGGGC GGCCTGGACC TCCCTTCAAC ATCACTCCAC GGAGAGCCAG AAGGTTTTTC
    CTGCGTTGCA TTATCTGGAA CACCAAGGAT GTGATCCTGG ACGACCTCAG CATCACAGGA
    GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGGTTG GCTTTGAAGA ACACAAGCAA
    AAGACAGATG TGCATTATCG GTCCCTGGGA GGCGAAGGGA ACTTTAACTG GAGGTTCATT
    TTCCCCTTTG ACTACCTGCT GGCTGAGCAA GTCTGCACCG TCTCCAAGAA GGATGCTTTC
    TGGAAGCTGG ACAAGACCGA GAGCAAAATC CCAGCACGAG TGGTATTCCA GATCTGGGAC
    AATGACAAGT TCTCCTTTGA TGATTTCCTG GGCTCCTTGC AGCTGGATCT CAACCGCATG
    CCCAAGCCTG CCAAGACAGC CGAGAAGTGC TCCCTGGACC AGCTGGATGA CACTTTCCAC
    CCAGAGTGGT TTGTGTCCCT TTTTGAACAG AAAACTGTGA AGGGCTGGTG GCCCTGTGTG
    GCGGAAGAGG GTGAGAAGAA GAAGCTGGCG GGCAAGCTGG AGATGACCTT GGAGATTGTG
    GCGGAGAGTG AGCATGAGGA GCGGCCCGCT GGCCAGGGCC GGGATGAGCC CAACATGAAC
    CCCAAGCTCG AGGACCCAAG GCGCCCTGAC ACCTCCTTCC TCTGGTTCAC CTCCCCGTAC
    AAGACTATGA AGTTCATCCT GTGGCGGCGT TTCCGGTGTG CCATCATCCT CTTCATCATC
    CTCTTCATCC TACTGCTGTT CTTGGGCATC TTCGTTTACG CCTTCCCGAA CTATGCTGCC
    ATGAAGCTGG TGAAGCCCTT CAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023474688.1
    CDS525..6971
    Translation

    Target ORF information:

    RefSeq Version XM_023618920.1
    Organism Equus caballus(horse)
    Definition Equus caballus dysferlin (DYSF), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023618920.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGAGGG TCTTTTTCCT TTACGCCGAG AACGTCCACA CCCCGGACAC CGACATCAGC 
    GATGCCTACT GCTCCGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAAAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGGGT CCCCCTGGAC
    CAGAGCTCTG AGCTTCACGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
    CTGGGAGAAG CCAAGGTCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CGCCTCTCCC TGGAGCTGTG CCCCTGTTCC CACCCTCTGT GCCGCTGGAA
    CCCTCCCCGA CTCTGCCCGA TGTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCCGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAATGGCAG
    GCCCCCACGG ATACAGGAGG AGAGGAGGAC ACCGAGGACC AGGGGCTCAC GGGAGATGAG
    GCAGAGCCAT TCTTGGATCA GAACGGAGCC CCAGGCCCCG GGGCTCCCAC CACCCTGAAG
    AAGCCACCTT CCCATCCTCC CCCCTACCAT CCTGGGGGGA AAAGGAAGAG AAGCACGCCT
    GCGCCCAGAA AGCTGCTTTC GGATAAACCA CAGGACTTCC AGATCAGGGT CCAGGTGATC
    GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC CGCGGCCAGG
    CAGACCAAGC GGACTCGGAT TCACAGGGGA AACAGCCCGC TCTTCAACGA GACTCTCTTC
    TTCAACGTGT TTGACTCTCC CTCGGAGCTG TTTGACGAGG CCGTCTTTAT CACGGTGGTA
    GACTCCTGTT CGCTCCGGAC AGATGCCCTC ATCGGGGAGT TCCGGATGGA TGTGGGTACC
    ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTGCTCTC GGACCCTGAC
    GATTTCTCTG CTGGGCCCAA AGGCTACCTG AAAGCAAGCC TTTGTGTGCT GGGGCCTGGA
    GACGAAGCTC CGCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAGCAAT
    CTGCTCAGGC CAACTGGCAT GGCCCTTCGA GGAGCGCACT TCTGTCTGAA GGTCTTCAGG
    GCTGAGGACT TACCACAGAT GGACGATGCC GTGGTGGACA GCGTGAAGCA GATCTTCGGC
    TTTGACAGTA ACAAGAAGAA CCTGGTGGAT CCCTTCGTCG AGGTCAGCTT TGCGGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGATG GCCAACCCTC AGTGGAACCA GAGCATCACG
    CTGCCTGTCA TGTTTCCCTC CATGTCTGAA AAAATGAGGA TTCGTGTCAT AGACTGGGAC
    CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA ATATGTCGAA AATCTCTGCC
    CCTGGAGGAG AAATAGCAGA GGAGCCTGCA GGTGTGGTCA AGCCTCCTCC AGCCACGGAC
    CTGGACGACC ACCTGGGCTT CCTCCCCACC TTCGGGCCCT GCTATGTCAA CCTCTACGGC
    AGCCCCAGGG AGTTCACTGG CTTCCCAGAC CCCTATGCAG AGCTCAACAC GGGCAAGGGA
    GAAGGCGTGG CCTACCGAGG CCGGCTTCTG GTCTCCCTGG AGACCAAACT GGTGGAGCAA
    GGCGAGCAGA AGGTGGAGGA TCTCGCTGCA GATGACATCC TTCGGGTGGA GAAGTACCTC
    CGGAGGCGCA AGTACTCCCT CTTTGCTGCC TTCTACTCGG CCTGCATGCT GCAGGATGTC
    GACGATGCCA TCCAGTTCGA GGTCAGCATC GGAAACTACG GGAACAAGTT TGACACGACC
    TGCCTGCCGC TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGACGG GTGCCACTAC
    TACTACTTAC CCTGGGGCAA CGTGAAGCCT GTGGTGGTGC TGTCATCCTA CTGGGAAGAC
    ATCAGCCACA GAGTCGACAC TCAGAACCAG CTGCTCCGGA TCGCCGACCG GCTGGAAGCC
    GGCCTGGAGC AGGTCCACCT GGCCCTGAAG GCGCGGTGCT CCACCGAGGA TGTGGACTCC
    CTGGTGGCTC AGCTGATGGA TGAGCTCATT GCAGACTGCA GCCAGCCCCT GTGCAACGTC
    CAAGAGACGC CCTCTGCCAC CCACCTGGAC CAGTACCTGT ACCGGCTGCG CACCCGTCAC
    CTGAGCCAGA TCAGTGAGGC GGCCCTGGCC TTGAAGCTCG GCCACAGTGA GCTCCCCGCC
    GCCCTTGAGC AGGCTGAGGA CTGGCTCCTG CGTCTCCGTG CCCTGGCGGA GGAGCCCCAG
    AACAGCCTGC CTGACGTGGT CATCTGGATG CTGCAGGGAG ACAAGCGGGT GGCGTACCAG
    CGGGTGCCCG CCCACGAGGT CCTCTTCTCC CGGCGGGGCG CCAACTACTG TGGCAAGAAT
    TGTGGGAAGC TGCAGACGAT CTTTCTGAAG TATCCAATGG AAGGGGTGCC GGGAGCCCGG
    ATGCCGGTGC AGATACGGGT GAAGCTCTGG TTCGGGCTCT CTGTGGATGA GAAGGAGTTC
    AATCAGTTTG CTGAGGGCAA GCTCTCTGTC TTCGCTGAAA CCTACGAGAA CCAGACCAAG
    CTGGCCCTAG TCGGGAACTG GGGCACAACG GGCCTCACCT ACCCCAAGTT TTCTGACGTC
    ACAGGCAAGA TCAAGCTACC CAAGGACAGC TTCCGCCCCT CGGCCGGCTG GGCCTGGGCT
    GGAGAGTGGT TCGTGTGTCC GGAGAAGACC CTACTCCACG ACACCGATGC GGGCCACCTG
    AGCTTTGTGG AGGAGGTGTT TGAGAACCAG ACCCGGCTGC CCGGAGGCCA GTGGATCTAC
    ATGAGTGACA ACTACACAGA TGTGAACGGG GAGAAGGTGC TTCCCAAGGA TGACATCGAG
    TGCCCACTGG GCTGGAAGTG GGAAGATGAA GAATGGTCCA CAGACCTCAA TCGGGCCGTT
    GACGAGCAAG GCTGGGAGTA CAGCATCACC ATCCCTCCGG AACGGAAGCC AAGGCACTGG
    GTCCCTGCCG AGAAGATGTA CTACACACAC CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG
    AGGGACCTCA GCCAGATGGA GGCGCTGAAG AGGCACAGGC AGGCGGAGGC GGAGGGCGAG
    GGCTGGGAGT ACGCCTCGCT CTTCGGCTGG AAGTTCCACC TGGAGTACCG CAAGACCGAT
    GCCTTCCGCC GCCGCCGCTG GCGCCGCCGC ATGGAGCCAC TGGAGAAGAC AGGGCCTGCA
    GCTGTGTTTG CCCTCGAGGG GGCCCTGGGC GGCGTGGTGG ACGACAGGAG TGAAGATTCG
    GTGTCCGTCT CCACCTTGAG CTTCGGCGTG AACAGACCCA CGATTTCCTG CATCTTCGAC
    TACGGGAACC GCTACCATCT ACGCTGCTAC ATGTACCAGG CCCGGGACCT GCCCGCCATG
    GACAAGGACT CTTTTTCTGA TCCCTACGCA ATCGTCTCCT TCCTGCACCA GAGCCAGAAG
    ACAGTGGTGG TGAAGAACAC CCTGAACCCC ACCTGGGACC AGACACTCAT CTTCTATGAG
    ATCGAGATCT TTGGTGAGCC GCCCAGCATC GCCGAGCAGC CGCCCAGCAT CGTGGTGGAG
    CTATACGACC ATGACACCTA CGGTGCAGAT GAGTTTATGG GACGCTGCAT CTGTCAGCCG
    ACTCTGGAAC GGATGCCCCG GCTGACCTGG TTCCCACTGA CTAGGGGCAG CCAGCCGGCG
    GGCGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCGGCCAT CTACCACATT
    CCTGGTTTTG AGGTGCAGGA TACATCAGGG ATCCTGGAAG AGCTGTGTGC ACCTGCCTTC
    TTCCTCCAGG GCCTCCTCCA AACGTCGGAG GACACAGACC TGCCCTACCC GCCACCCCAG
    AGAGAGGCCA ACATCTACAT GGTTCCCCAG AACATCAAGC CTGCTCTCCA GCGGACGGCC
    ATCGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCACCTGGC CAGTGTCTCC
    TCCCCCAGCC TCGTGGTGGA GTGCGGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC
    CTCCAGAAGA ACCCCAACTT CGACGTCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG
    GAGGAGCTCT ACTGTCCCCC CATCGTGGTC AAGGTCATCG ATAACTGCCA GTTTGGCCGC
    CGGCCTGTGG TGGGCCAGTG TACCATCTGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC
    TCAGAGGAGA GTCCGTCCCC ACAGGGCGGC CCAGATGATG TGAGCTTACT CAGTCCTGGG
    GAAGATGTGC TCATTGACAT TGATGACAAG GAGCCCCTCA TCCCTATGCA GCTTGCAGAT
    GGTCTGTCGG GCTCGGCCCC CACTAACATG GCATCTTCTC CGTCCAGTCC TCATGAGGAG
    GAGTTCATCG ATTGGTGGAG CAAATTCTTT GCCTCGATAG GGGAGAGGGA AAAGTGCGGG
    TCCTACTTGG AGAAGGACTT TGACACTCTA AAGGTCTATG ACACACAACT GGAGAATGTG
    GAGGCCTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTCTACCG GGGCAAGACT
    CAGGAGGAGA TGGAAGACCC ATCTGTCGTT GGGGAATTTA AGGGCCTCTT CAAAATTTAT
    CCCCTCCCAG AAGACCCGGC CATCCCCATG CCCCCAAGAC AGTTCCACCA GCTAGCTGCC
    CAGGGACCCC AGGAGTGCCT GGTCCGCATC TACATCATCC GGGCATTCGG CCTGCAGCCC
    AAGGACCCCA ATGGGAAGTG TGACCCTTAC ATCAAGATCT CCATAGGGAA GAAATCCGTG
    AGCGACCAGG ATAACTACAT CCCCTGCACT CTGGAGCCTG TGTTTGGAAA GATGTTCGAA
    CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGATCA CTCTCTATGA CTATGACCTC
    CTCTCCAAGG ACGAGATGAT CGGGGAGACG GTCATCGACC TGGAGAATAG GCTGCTGTCC
    AAGTTTGGAG CTCGCTGCGG ACTCCCACAG ACCTACTGTG TCTCTGGACC GAACCAGTGG
    AGGGACCAGC TCCGCCCCTC CCAGCTTCTC CACCTGTTCT GCCAGCAGCA GAGAGTCAAG
    GCCCCCGTGT ACCAGACCGA CCGTGTGGTG TTTGGGAATA AAGAATACAC GATTGAAGAA
    ATAGAGGCTG GGAGGGTCCT GAATCCACAC CTGGGCCCAG TGGAAGAGCG CCTGGCCTTG
    CACGTCCTCC AGCAGCAGGG CTTGGTGCCC GAGCATGTGG AGTCCCGGCC CCTCTATAGC
    CCCCTGCAGC CGGACATTGA GCAGGGGAAG CTGCAGATGT GGATCGACCT GTTTCCAAAG
    GCCCTGGGGC GGCCTGGACC TCCCTTCAAC ATCACTCCAC GGAGAGCCAG AAGGTTTTTC
    CTGCGTTGCA TTATCTGGAA CACCAAGGAT GTGATCCTGG ACGACCTCAG CATCACAGGA
    GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGGTTG GCTTTGAAGA ACACAAGCAA
    AAGACAGATG TGCATTATCG GTCCCTGGGA GGCGAAGGGA ACTTTAACTG GAGGTTCATT
    TTCCCCTTTG ACTACCTGCT GGCTGAGCAA GTCTGCACCG TCTCCAAGAA GGATGCTTTC
    TGGAAGCTGG ACAAGACCGA GAGCAAAATC CCAGCACGAG TGGTATTCCA GATCTGGGAC
    AATGACAAGT TCTCCTTTGA TGATTTCCTG GGCTCCTTGC AGCTGGATCT CAACCGCATG
    CCCAAGCCTG CCAAGACAGC CGAGAAGTGC TCCCTGGACC AGCTGGATGA CACTTTCCAC
    CCAGAGTGGT TTGTGTCCCT TTTTGAACAG AAAACTGTGA AGGGCTGGTG GCCCTGTGTG
    GCGGAAGAGG GTGAGAAGAA GAAGCTGGCG GGCAAGCTGG AGATGACCTT GGAGATTGTG
    GCGGAGAGTG AGCATGAGGA GCGGCCCGCT GGCCAGGGCC GGGATGAGCC CAACATGAAC
    CCCAAGCTCG AGGACCCAAG GCGCCCTGAC ACCTCCTTCC TCTGGTTCAC CTCCCCGTAC
    AAGACTATGA AGTTCATCCT GTGGCGGCGT TTCCGGTGTG CCATCATCCT CTTCATCATC
    CTCTTCATCC TACTGCTGTT CTTGGGCATC TTCGTTTACG CCTTCCCGAA CTATGCTGCC
    ATGAAGCTGG TGAAGCCCTT CAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb49789
    Clone ID Related Accession (Same CDS sequence) XM_023618921.1
    Accession Version XM_023618921.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6363bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product dysferlin isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009158.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGAGGG TCTTTTTCCT TTACGCCGAG AACGTCCACA CCCCGGACAC CGACATCAGC 
    GATGCCTACT GCTCCGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAAAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGGGT CCCCCTGGAC
    CAGAGCTCTG AGCTTCACGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
    CTGGGAGAAG CCAAGGTCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CGCCTCTCCC TGGAGCTGTG CCCCTGTTCC CACCCTCTGT GCCGCTGGAA
    CCCTCCCCGA CTCTGCCCGA TGTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCCGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAATGGCAG
    GCCCCCACGG ATACAGGAGG AGAGGAGGAC ACCGAGGACC AGGGGCTCAC GGGAGATGAG
    GCAGAGCCAT TCTTGGATCA GAACGGAGCC CCAGGCCCCG GGGCTCCCAC CACCCTGAAG
    AAGCCACCTT CCCATCCTCC CCCCTACCAT CCTGGGGGGA AAAGGAAGAG AAGCACGCCT
    GCGCCCAGAA AGCTGCTTTC GGATAAACCA CAGGACTTCC AGATCAGGGT CCAGGTGATC
    GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC CGCGGCCAGG
    CAGACCAAGC GGACTCGGAT TCACAGGGGA AACAGCCCGC TCTTCAACGA GACTCTCTTC
    TTCAACGTGT TTGACTCTCC CTCGGAGCTG TTTGACGAGG CCGTCTTTAT CACGGTGGTA
    GACTCCTGTT CGCTCCGGAC AGATGCCCTC ATCGGGGAGT TCCGGATGGA TGTGGGTACC
    ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTGCTCTC GGACCCTGAC
    GATTTCTCTG CTGGGCCCAA AGGCTACCTG AAAGCAAGCC TTTGTGTGCT GGGGCCTGGA
    GACGAAGCTC CGCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAGCAAT
    CTGCTCAGGC CAACTGGCAT GGCCCTTCGA GGAGCGCACT TCTGTCTGAA GGTCTTCAGG
    GCTGAGGACT TACCACAGAT GGACGATGCC GTGGTGGACA GCGTGAAGCA GATCTTCGGC
    TTTGACAGTA ACAAGAAGAA CCTGGTGGAT CCCTTCGTCG AGGTCAGCTT TGCGGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGATG GCCAACCCTC AGTGGAACCA GAGCATCACG
    CTGCCTGTCA TGTTTCCCTC CATGTCTGAA AAAATGAGGA TTCGTGTCAT AGACTGGGAC
    CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA ATATGTCGAA AATCTCTGCC
    CCTGGAGGAG AAATAGCAGT GGACGACCAC CTGGGCTTCC TCCCCACCTT CGGGCCCTGC
    TATGTCAACC TCTACGGCAG CCCCAGGGAG TTCACTGGCT TCCCAGACCC CTATGCAGAG
    CTCAACACGG GCAAGGGAGA AGGCGTGGCC TACCGAGGCC GGCTTCTGGT CTCCCTGGAG
    ACCAAACTGG TGGAGCAAGG CGAGCAGAAG GTGGAGGATC TCGCTGCAGA TGACATCCTT
    CGGGTGGAGA AGTACCTCCG GAGGCGCAAG TACTCCCTCT TTGCTGCCTT CTACTCGGCC
    TGCATGCTGC AGGATGTCGA CGATGCCATC CAGTTCGAGG TCAGCATCGG AAACTACGGG
    AACAAGTTTG ACACGACCTG CCTGCCGCTG GCCTCCACCA CCCAGTACAG CCGGGCGGTC
    TTTGACGGGT GCCACTACTA CTACTTACCC TGGGGCAACG TGAAGCCTGT GGTGGTGCTG
    TCATCCTACT GGGAAGACAT CAGCCACAGA GTCGACACTC AGAACCAGCT GCTCCGGATC
    GCCGACCGGC TGGAAGCCGG CCTGGAGCAG GTCCACCTGG CCCTGAAGGC GCGGTGCTCC
    ACCGAGGATG TGGACTCCCT GGTGGCTCAG CTGATGGATG AGCTCATTGC AGACTGCAGC
    CAGCCCCTGT GCAACGTCCA AGAGACGCCC TCTGCCACCC ACCTGGACCA GTACCTGTAC
    CGGCTGCGCA CCCGTCACCT GAGCCAGATC AGTGAGGCGG CCCTGGCCTT GAAGCTCGGC
    CACAGTGAGC TCCCCGCCGC CCTTGAGCAG GCTGAGGACT GGCTCCTGCG TCTCCGTGCC
    CTGGCGGAGG AGCCCCAGAA CAGCCTGCCT GACGTGGTCA TCTGGATGCT GCAGGGAGAC
    AAGCGGGTGG CGTACCAGCG GGTGCCCGCC CACGAGGTCC TCTTCTCCCG GCGGGGCGCC
    AACTACTGTG GCAAGAATTG TGGGAAGCTG CAGACGATCT TTCTGAAGTA TCCAATGGAA
    GGGGTGCCGG GAGCCCGGAT GCCGGTGCAG ATACGGGTGA AGCTCTGGTT CGGGCTCTCT
    GTGGATGAGA AGGAGTTCAA TCAGTTTGCT GAGGGCAAGC TCTCTGTCTT CGCTGAAACC
    TACGAGAACC AGACCAAGCT GGCCCTAGTC GGGAACTGGG GCACAACGGG CCTCACCTAC
    CCCAAGTTTT CTGACGTCAC AGGCAAGATC AAGCTACCCA AGGACAGCTT CCGCCCCTCG
    GCCGGCTGGG CCTGGGCTGG AGAGTGGTTC GTGTGTCCGG AGAAGACCCT ACTCCACGAC
    ACCGATGCGG GCCACCTGAG CTTTGTGGAG GAGGTGTTTG AGAACCAGAC CCGGCTGCCC
    GGAGGCCAGT GGATCTACAT GAGTGACAAC TACACAGATG TGAACGGGGA GAAGGTGCTT
    CCCAAGGATG ACATCGAGTG CCCACTGGGC TGGAAGTGGG AAGATGAAGA ATGGTCCACA
    GACCTCAATC GGGCCGTTGA CGAGCAAGGC TGGGAGTACA GCATCACCAT CCCTCCGGAA
    CGGAAGCCAA GGCACTGGGT CCCTGCCGAG AAGATGTACT ACACACACCG TCGGCGGCGC
    TGGGTCCGCC TGCGCAGGAG GGACCTCAGC CAGATGGAGG CGCTGAAGAG GCACAGGCAG
    GCGGAGGCGG AGGGCGAGGG CTGGGAGTAC GCCTCGCTCT TCGGCTGGAA GTTCCACCTG
    GAGTACCGCA AGACCGATGC CTTCCGCCGC CGCCGCTGGC GCCGCCGCAT GGAGCCACTG
    GAGAAGACAG GGCCTGCAGC TGTGTTTGCC CTCGAGGGGG CCCTGGGCGG CGTGGTGGAC
    GACAGGAGTG AAGATTCGGT GTCCGTCTCC ACCTTGAGCT TCGGCGTGAA CAGACCCACG
    ATTTCCTGCA TCTTCGACTA CGGGAACCGC TACCATCTAC GCTGCTACAT GTACCAGGCC
    CGGGACCTGC CCGCCATGGA CAAGGACTCT TTTTCTGATC CCTACGCAAT CGTCTCCTTC
    CTGCACCAGA GCCAGAAGAC AGTGGTGGTG AAGAACACCC TGAACCCCAC CTGGGACCAG
    ACACTCATCT TCTATGAGAT CGAGATCTTT GGTGAGCCGC CCAGCATCGC CGAGCAGCCG
    CCCAGCATCG TGGTGGAGCT ATACGACCAT GACACCTACG GTGCAGATGA GTTTATGGGA
    CGCTGCATCT GTCAGCCGAC TCTGGAACGG ATGCCCCGGC TGACCTGGTT CCCACTGACT
    AGGGGCAGCC AGCCGGCGGG CGAGCTGCTG GCCTCTTTTG AGCTCATCCA GAGAGAGAAG
    CCGGCCATCT ACCACATTCC TGGTTTTGAG GTGCAGGATA CATCAGGGAT CCTGGAAGAG
    TCGGAGGACA CAGACCTGCC CTACCCGCCA CCCCAGAGAG AGGCCAACAT CTACATGGTT
    CCCCAGAACA TCAAGCCTGC TCTCCAGCGG ACGGCCATCG AGATCCTGGC ATGGGGCCTG
    CGGAACATGA AGAGTTACCA CCTGGCCAGT GTCTCCTCCC CCAGCCTCGT GGTGGAGTGC
    GGGGGCCAGA CGGTGCAGTC CTGTGTCATC AGGAACCTCC AGAAGAACCC CAACTTCGAC
    GTCTGCACCC TCTTCATGGA AGTGATGCTG CCCAGGGAGG AGCTCTACTG TCCCCCCATC
    GTGGTCAAGG TCATCGATAA CTGCCAGTTT GGCCGCCGGC CTGTGGTGGG CCAGTGTACC
    ATCTGCTCCC TGGAGAGCTT CCTGTGTGAC CCCTACTCAG AGGAGAGTCC GTCCCCACAG
    GGCGGCCCAG ATGATGTGAG CTTACTCAGT CCTGGGGAAG ATGTGCTCAT TGACATTGAT
    GACAAGGAGC CCCTCATCCC TATGCAGCTT GCAGATGGTC TGTCGGGCTC GGCCCCCACT
    AACATGGCAT CTTCTCCGTC CAGTCCTCAT GAGGAGGAGT TCATCGATTG GTGGAGCAAA
    TTCTTTGCCT CGATAGGGGA GAGGGAAAAG TGCGGGTCCT ACTTGGAGAA GGACTTTGAC
    ACTCTAAAGG TCTATGACAC ACAACTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC
    TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGATGGA AGACCCATCT
    GTCGTTGGGG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCGGCCATC
    CCCATGCCCC CAAGACAGTT CCACCAGCTA GCTGCCCAGG GACCCCAGGA GTGCCTGGTC
    CGCATCTACA TCATCCGGGC ATTCGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAC
    CCTTACATCA AGATCTCCAT AGGGAAGAAA TCCGTGAGCG ACCAGGATAA CTACATCCCC
    TGCACTCTGG AGCCTGTGTT TGGAAAGATG TTCGAACTGA CCTGCACTCT GCCTCTGGAG
    AAGGACCTGA AGATCACTCT CTATGACTAT GACCTCCTCT CCAAGGACGA GATGATCGGG
    GAGACGGTCA TCGACCTGGA GAATAGGCTG CTGTCCAAGT TTGGAGCTCG CTGCGGACTC
    CCACAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG
    CTTCTCCACC TGTTCTGCCA GCAGCAGAGA GTCAAGGCCC CCGTGTACCA GACCGACCGT
    GTGGTGTTTG GGAATAAAGA ATACACGATT GAAGAAATAG AGGCTGGGAG GGTCCTGAAT
    CCACACCTGG GCCCAGTGGA AGAGCGCCTG GCCTTGCACG TCCTCCAGCA GCAGGGCTTG
    GTGCCCGAGC ATGTGGAGTC CCGGCCCCTC TATAGCCCCC TGCAGCCGGA CATTGAGCAG
    GGGAAGCTGC AGATGTGGAT CGACCTGTTT CCAAAGGCCC TGGGGCGGCC TGGACCTCCC
    TTCAACATCA CTCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGCATTAT CTGGAACACC
    AAGGATGTGA TCCTGGACGA CCTCAGCATC ACAGGAGAGA AGATGAGCGA CATTTATGTG
    AAAGGTTGGA TGGTTGGCTT TGAAGAACAC AAGCAAAAGA CAGATGTGCA TTATCGGTCC
    CTGGGAGGCG AAGGGAACTT TAACTGGAGG TTCATTTTCC CCTTTGACTA CCTGCTGGCT
    GAGCAAGTCT GCACCGTCTC CAAGAAGGAT GCTTTCTGGA AGCTGGACAA GACCGAGAGC
    AAAATCCCAG CACGAGTGGT ATTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT
    TTCCTGGGCT CCTTGCAGCT GGATCTCAAC CGCATGCCCA AGCCTGCCAA GACAGCCGAG
    AAGTGCTCCC TGGACCAGCT GGATGACACT TTCCACCCAG AGTGGTTTGT GTCCCTTTTT
    GAACAGAAAA CTGTGAAGGG CTGGTGGCCC TGTGTGGCGG AAGAGGGTGA GAAGAAGAAG
    CTGGCGGGCA AGCTGGAGAT GACCTTGGAG ATTGTGGCGG AGAGTGAGCA TGAGGAGCGG
    CCCGCTGGCC AGGGCCGGGA TGAGCCCAAC ATGAACCCCA AGCTCGAGGA CCCAAGGCGC
    CCTGACACCT CCTTCCTCTG GTTCACCTCC CCGTACAAGA CTATGAAGTT CATCCTGTGG
    CGGCGTTTCC GGTGTGCCAT CATCCTCTTC ATCATCCTCT TCATCCTACT GCTGTTCTTG
    GGCATCTTCG TTTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023474689.1
    CDS525..6887
    Translation

    Target ORF information:

    RefSeq Version XM_023618921.1
    Organism Equus caballus(horse)
    Definition Equus caballus dysferlin (DYSF), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023618921.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGAGGG TCTTTTTCCT TTACGCCGAG AACGTCCACA CCCCGGACAC CGACATCAGC 
    GATGCCTACT GCTCCGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAAAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGGGT CCCCCTGGAC
    CAGAGCTCTG AGCTTCACGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
    CTGGGAGAAG CCAAGGTCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CGCCTCTCCC TGGAGCTGTG CCCCTGTTCC CACCCTCTGT GCCGCTGGAA
    CCCTCCCCGA CTCTGCCCGA TGTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCCGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAATGGCAG
    GCCCCCACGG ATACAGGAGG AGAGGAGGAC ACCGAGGACC AGGGGCTCAC GGGAGATGAG
    GCAGAGCCAT TCTTGGATCA GAACGGAGCC CCAGGCCCCG GGGCTCCCAC CACCCTGAAG
    AAGCCACCTT CCCATCCTCC CCCCTACCAT CCTGGGGGGA AAAGGAAGAG AAGCACGCCT
    GCGCCCAGAA AGCTGCTTTC GGATAAACCA CAGGACTTCC AGATCAGGGT CCAGGTGATC
    GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC CGCGGCCAGG
    CAGACCAAGC GGACTCGGAT TCACAGGGGA AACAGCCCGC TCTTCAACGA GACTCTCTTC
    TTCAACGTGT TTGACTCTCC CTCGGAGCTG TTTGACGAGG CCGTCTTTAT CACGGTGGTA
    GACTCCTGTT CGCTCCGGAC AGATGCCCTC ATCGGGGAGT TCCGGATGGA TGTGGGTACC
    ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTGCTCTC GGACCCTGAC
    GATTTCTCTG CTGGGCCCAA AGGCTACCTG AAAGCAAGCC TTTGTGTGCT GGGGCCTGGA
    GACGAAGCTC CGCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAGCAAT
    CTGCTCAGGC CAACTGGCAT GGCCCTTCGA GGAGCGCACT TCTGTCTGAA GGTCTTCAGG
    GCTGAGGACT TACCACAGAT GGACGATGCC GTGGTGGACA GCGTGAAGCA GATCTTCGGC
    TTTGACAGTA ACAAGAAGAA CCTGGTGGAT CCCTTCGTCG AGGTCAGCTT TGCGGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGATG GCCAACCCTC AGTGGAACCA GAGCATCACG
    CTGCCTGTCA TGTTTCCCTC CATGTCTGAA AAAATGAGGA TTCGTGTCAT AGACTGGGAC
    CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA ATATGTCGAA AATCTCTGCC
    CCTGGAGGAG AAATAGCAGT GGACGACCAC CTGGGCTTCC TCCCCACCTT CGGGCCCTGC
    TATGTCAACC TCTACGGCAG CCCCAGGGAG TTCACTGGCT TCCCAGACCC CTATGCAGAG
    CTCAACACGG GCAAGGGAGA AGGCGTGGCC TACCGAGGCC GGCTTCTGGT CTCCCTGGAG
    ACCAAACTGG TGGAGCAAGG CGAGCAGAAG GTGGAGGATC TCGCTGCAGA TGACATCCTT
    CGGGTGGAGA AGTACCTCCG GAGGCGCAAG TACTCCCTCT TTGCTGCCTT CTACTCGGCC
    TGCATGCTGC AGGATGTCGA CGATGCCATC CAGTTCGAGG TCAGCATCGG AAACTACGGG
    AACAAGTTTG ACACGACCTG CCTGCCGCTG GCCTCCACCA CCCAGTACAG CCGGGCGGTC
    TTTGACGGGT GCCACTACTA CTACTTACCC TGGGGCAACG TGAAGCCTGT GGTGGTGCTG
    TCATCCTACT GGGAAGACAT CAGCCACAGA GTCGACACTC AGAACCAGCT GCTCCGGATC
    GCCGACCGGC TGGAAGCCGG CCTGGAGCAG GTCCACCTGG CCCTGAAGGC GCGGTGCTCC
    ACCGAGGATG TGGACTCCCT GGTGGCTCAG CTGATGGATG AGCTCATTGC AGACTGCAGC
    CAGCCCCTGT GCAACGTCCA AGAGACGCCC TCTGCCACCC ACCTGGACCA GTACCTGTAC
    CGGCTGCGCA CCCGTCACCT GAGCCAGATC AGTGAGGCGG CCCTGGCCTT GAAGCTCGGC
    CACAGTGAGC TCCCCGCCGC CCTTGAGCAG GCTGAGGACT GGCTCCTGCG TCTCCGTGCC
    CTGGCGGAGG AGCCCCAGAA CAGCCTGCCT GACGTGGTCA TCTGGATGCT GCAGGGAGAC
    AAGCGGGTGG CGTACCAGCG GGTGCCCGCC CACGAGGTCC TCTTCTCCCG GCGGGGCGCC
    AACTACTGTG GCAAGAATTG TGGGAAGCTG CAGACGATCT TTCTGAAGTA TCCAATGGAA
    GGGGTGCCGG GAGCCCGGAT GCCGGTGCAG ATACGGGTGA AGCTCTGGTT CGGGCTCTCT
    GTGGATGAGA AGGAGTTCAA TCAGTTTGCT GAGGGCAAGC TCTCTGTCTT CGCTGAAACC
    TACGAGAACC AGACCAAGCT GGCCCTAGTC GGGAACTGGG GCACAACGGG CCTCACCTAC
    CCCAAGTTTT CTGACGTCAC AGGCAAGATC AAGCTACCCA AGGACAGCTT CCGCCCCTCG
    GCCGGCTGGG CCTGGGCTGG AGAGTGGTTC GTGTGTCCGG AGAAGACCCT ACTCCACGAC
    ACCGATGCGG GCCACCTGAG CTTTGTGGAG GAGGTGTTTG AGAACCAGAC CCGGCTGCCC
    GGAGGCCAGT GGATCTACAT GAGTGACAAC TACACAGATG TGAACGGGGA GAAGGTGCTT
    CCCAAGGATG ACATCGAGTG CCCACTGGGC TGGAAGTGGG AAGATGAAGA ATGGTCCACA
    GACCTCAATC GGGCCGTTGA CGAGCAAGGC TGGGAGTACA GCATCACCAT CCCTCCGGAA
    CGGAAGCCAA GGCACTGGGT CCCTGCCGAG AAGATGTACT ACACACACCG TCGGCGGCGC
    TGGGTCCGCC TGCGCAGGAG GGACCTCAGC CAGATGGAGG CGCTGAAGAG GCACAGGCAG
    GCGGAGGCGG AGGGCGAGGG CTGGGAGTAC GCCTCGCTCT TCGGCTGGAA GTTCCACCTG
    GAGTACCGCA AGACCGATGC CTTCCGCCGC CGCCGCTGGC GCCGCCGCAT GGAGCCACTG
    GAGAAGACAG GGCCTGCAGC TGTGTTTGCC CTCGAGGGGG CCCTGGGCGG CGTGGTGGAC
    GACAGGAGTG AAGATTCGGT GTCCGTCTCC ACCTTGAGCT TCGGCGTGAA CAGACCCACG
    ATTTCCTGCA TCTTCGACTA CGGGAACCGC TACCATCTAC GCTGCTACAT GTACCAGGCC
    CGGGACCTGC CCGCCATGGA CAAGGACTCT TTTTCTGATC CCTACGCAAT CGTCTCCTTC
    CTGCACCAGA GCCAGAAGAC AGTGGTGGTG AAGAACACCC TGAACCCCAC CTGGGACCAG
    ACACTCATCT TCTATGAGAT CGAGATCTTT GGTGAGCCGC CCAGCATCGC CGAGCAGCCG
    CCCAGCATCG TGGTGGAGCT ATACGACCAT GACACCTACG GTGCAGATGA GTTTATGGGA
    CGCTGCATCT GTCAGCCGAC TCTGGAACGG ATGCCCCGGC TGACCTGGTT CCCACTGACT
    AGGGGCAGCC AGCCGGCGGG CGAGCTGCTG GCCTCTTTTG AGCTCATCCA GAGAGAGAAG
    CCGGCCATCT ACCACATTCC TGGTTTTGAG GTGCAGGATA CATCAGGGAT CCTGGAAGAG
    TCGGAGGACA CAGACCTGCC CTACCCGCCA CCCCAGAGAG AGGCCAACAT CTACATGGTT
    CCCCAGAACA TCAAGCCTGC TCTCCAGCGG ACGGCCATCG AGATCCTGGC ATGGGGCCTG
    CGGAACATGA AGAGTTACCA CCTGGCCAGT GTCTCCTCCC CCAGCCTCGT GGTGGAGTGC
    GGGGGCCAGA CGGTGCAGTC CTGTGTCATC AGGAACCTCC AGAAGAACCC CAACTTCGAC
    GTCTGCACCC TCTTCATGGA AGTGATGCTG CCCAGGGAGG AGCTCTACTG TCCCCCCATC
    GTGGTCAAGG TCATCGATAA CTGCCAGTTT GGCCGCCGGC CTGTGGTGGG CCAGTGTACC
    ATCTGCTCCC TGGAGAGCTT CCTGTGTGAC CCCTACTCAG AGGAGAGTCC GTCCCCACAG
    GGCGGCCCAG ATGATGTGAG CTTACTCAGT CCTGGGGAAG ATGTGCTCAT TGACATTGAT
    GACAAGGAGC CCCTCATCCC TATGCAGCTT GCAGATGGTC TGTCGGGCTC GGCCCCCACT
    AACATGGCAT CTTCTCCGTC CAGTCCTCAT GAGGAGGAGT TCATCGATTG GTGGAGCAAA
    TTCTTTGCCT CGATAGGGGA GAGGGAAAAG TGCGGGTCCT ACTTGGAGAA GGACTTTGAC
    ACTCTAAAGG TCTATGACAC ACAACTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC
    TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGATGGA AGACCCATCT
    GTCGTTGGGG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCGGCCATC
    CCCATGCCCC CAAGACAGTT CCACCAGCTA GCTGCCCAGG GACCCCAGGA GTGCCTGGTC
    CGCATCTACA TCATCCGGGC ATTCGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAC
    CCTTACATCA AGATCTCCAT AGGGAAGAAA TCCGTGAGCG ACCAGGATAA CTACATCCCC
    TGCACTCTGG AGCCTGTGTT TGGAAAGATG TTCGAACTGA CCTGCACTCT GCCTCTGGAG
    AAGGACCTGA AGATCACTCT CTATGACTAT GACCTCCTCT CCAAGGACGA GATGATCGGG
    GAGACGGTCA TCGACCTGGA GAATAGGCTG CTGTCCAAGT TTGGAGCTCG CTGCGGACTC
    CCACAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG
    CTTCTCCACC TGTTCTGCCA GCAGCAGAGA GTCAAGGCCC CCGTGTACCA GACCGACCGT
    GTGGTGTTTG GGAATAAAGA ATACACGATT GAAGAAATAG AGGCTGGGAG GGTCCTGAAT
    CCACACCTGG GCCCAGTGGA AGAGCGCCTG GCCTTGCACG TCCTCCAGCA GCAGGGCTTG
    GTGCCCGAGC ATGTGGAGTC CCGGCCCCTC TATAGCCCCC TGCAGCCGGA CATTGAGCAG
    GGGAAGCTGC AGATGTGGAT CGACCTGTTT CCAAAGGCCC TGGGGCGGCC TGGACCTCCC
    TTCAACATCA CTCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGCATTAT CTGGAACACC
    AAGGATGTGA TCCTGGACGA CCTCAGCATC ACAGGAGAGA AGATGAGCGA CATTTATGTG
    AAAGGTTGGA TGGTTGGCTT TGAAGAACAC AAGCAAAAGA CAGATGTGCA TTATCGGTCC
    CTGGGAGGCG AAGGGAACTT TAACTGGAGG TTCATTTTCC CCTTTGACTA CCTGCTGGCT
    GAGCAAGTCT GCACCGTCTC CAAGAAGGAT GCTTTCTGGA AGCTGGACAA GACCGAGAGC
    AAAATCCCAG CACGAGTGGT ATTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT
    TTCCTGGGCT CCTTGCAGCT GGATCTCAAC CGCATGCCCA AGCCTGCCAA GACAGCCGAG
    AAGTGCTCCC TGGACCAGCT GGATGACACT TTCCACCCAG AGTGGTTTGT GTCCCTTTTT
    GAACAGAAAA CTGTGAAGGG CTGGTGGCCC TGTGTGGCGG AAGAGGGTGA GAAGAAGAAG
    CTGGCGGGCA AGCTGGAGAT GACCTTGGAG ATTGTGGCGG AGAGTGAGCA TGAGGAGCGG
    CCCGCTGGCC AGGGCCGGGA TGAGCCCAAC ATGAACCCCA AGCTCGAGGA CCCAAGGCGC
    CCTGACACCT CCTTCCTCTG GTTCACCTCC CCGTACAAGA CTATGAAGTT CATCCTGTGG
    CGGCGTTTCC GGTGTGCCAT CATCCTCTTC ATCATCCTCT TCATCCTACT GCTGTTCTTG
    GGCATCTTCG TTTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb49790
    Clone ID Related Accession (Same CDS sequence) XM_023618922.1
    Accession Version XM_023618922.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6342bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product dysferlin isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009158.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGAGGG TCTTTTTCCT TTACGCCGAG AACGTCCACA CCCCGGACAC CGACATCAGC 
    GATGCCTACT GCTCCGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAAAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGGGT CCCCCTGGAC
    CAGAGCTCTG AGCTTCACGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
    CTGGGAGAAG CCAAGGTCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CGCCTCTCCC TGGAGCTGTG CCCCTGTTCC CACCCTCTGT GCCGCTGGAA
    CCCTCCCCGA CTCTGCCCGA TGTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCCGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAATGGCAG
    GCCCCCACGG ATACAGGAGG AGAGGAGGAC ACCGAGGACC AGGGGCTCAC GGGAGATGAG
    GCAGAGCCAT TCTTGGATCA GAACGGAGCC CCAGGCCCCG GGGCTCCCAC CACCCTGAAG
    AAGCCACCTT CCCATCCTCC CCCCTACCAT CCTGGGGGGA AAAGGAAGAG AAGCACGCCT
    GCGCCCAGAA AGCTGCTTTC GGATAAACCA CAGGACTTCC AGATCAGGGT CCAGGTGATC
    GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC CGCGGCCAGG
    CAGACCAAGC GGACTCGGAT TCACAGGGGA AACAGCCCGC TCTTCAACGA GACTCTCTTC
    TTCAACGTGT TTGACTCTCC CTCGGAGCTG TTTGACGAGG CCGTCTTTAT CACGGTGGTA
    GACTCCTGTT CGCTCCGGAC AGATGCCCTC ATCGGGGAGT TCCGGATGGA TGTGGGTACC
    ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTGCTCTC GGACCCTGAC
    GATTTCTCTG CTGGGCCCAA AGGCTACCTG AAAGCAAGCC TTTGTGTGCT GGGGCCTGGA
    GACGAAGCTC CGCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAGCAAT
    CTGCTCAGGC CAACTGGCAT GGCCCTTCGA GGAGCGCACT TCTGTCTGAA GGTCTTCAGG
    GCTGAGGACT TACCACAGAT GGACGATGCC GTGGTGGACA GCGTGAAGCA GATCTTCGGC
    TTTGACAGTA ACAAGAAGAA CCTGGTGGAT CCCTTCGTCG AGGTCAGCTT TGCGGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGATG GCCAACCCTC AGTGGAACCA GAGCATCACG
    CTGCCTGTCA TGTTTCCCTC CATGTCTGAA AAAATGAGGA TTCGTGTCAT AGACTGGGAC
    CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA ATATGTCGAA AATCTCTGCC
    CCTGGAGGAG AAATAGCAGA GGAGCCTGCA GGTGTGGTCA AGCCTCCTCC AGCCACGGAC
    CTGGACGACC ACCTGGGCTT CCTCCCCACC TTCGGGCCCT GCTATGTCAA CCTCTACGGC
    AGCCCCAGGG AGTTCACTGG CTTCCCAGAC CCCTATGCAG AGCTCAACAC GGGCAAGGGA
    GAAGGCGTGG CCTACCGAGG CCGGCTTCTG GTCTCCCTGG AGACCAAACT GGTGGAGCAA
    GGCGAGCAGA AGGTGGAGGA TCTCGCTGCA GATGACATCC TTCGGGTGGA GAAGTACCTC
    CGGAGGCGCA AGTACTCCCT CTTTGCTGCC TTCTACTCGG CCTGCATGCT GCAGGATGTC
    GACGATGCCA TCCAGTTCGA GGTCAGCATC GGAAACTACG GGAACAAGTT TGACACGACC
    TGCCTGCCGC TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGACGG GTGCCACTAC
    TACTACTTAC CCTGGGGCAA CGTGAAGCCT GTGGTGGTGC TGTCATCCTA CTGGGAAGAC
    ATCAGCCACA GAGTCGACAC TCAGAACCAG CTGCTCCGGA TCGCCGACCG GCTGGAAGCC
    GGCCTGGAGC AGGTCCACCT GGCCCTGAAG GCGCGGTGCT CCACCGAGGA TGTGGACTCC
    CTGGTGGCTC AGCTGATGGA TGAGCTCATT GCAGACTGCA GCCAGCCCCT GTGCAACGTC
    CAAGAGACGC CCTCTGCCAC CCACCTGGAC CAGTACCTGT ACCGGCTGCG CACCCGTCAC
    CTGAGCCAGA TCAGTGAGGC GGCCCTGGCC TTGAAGCTCG GCCACAGTGA GCTCCCCGCC
    GCCCTTGAGC AGGCTGAGGA CTGGCTCCTG CGTCTCCGTG CCCTGGCGGA GGAGCCCCAG
    AACAGCCTGC CTGACGTGGT CATCTGGATG CTGCAGGGAG ACAAGCGGGT GGCGTACCAG
    CGGGTGCCCG CCCACGAGGT CCTCTTCTCC CGGCGGGGCG CCAACTACTG TGGCAAGAAT
    TGTGGGAAGC TGCAGACGAT CTTTCTGAAG TATCCAATGG AAGGGGTGCC GGGAGCCCGG
    ATGCCGGTGC AGATACGGGT GAAGCTCTGG TTCGGGCTCT CTGTGGATGA GAAGGAGTTC
    AATCAGTTTG CTGAGGGCAA GCTCTCTGTC TTCGCTGAAA CCTACGAGAA CCAGACCAAG
    CTGGCCCTAG TCGGGAACTG GGGCACAACG GGCCTCACCT ACCCCAAGTT TTCTGACGTC
    ACAGGCAAGA TCAAGCTACC CAAGGACAGC TTCCGCCCCT CGGCCGGCTG GGCCTGGGCT
    GGAGAGTGGT TCGTGTGTCC GGAGAAGACC CTACTCCACG ACACCGATGC GGGCCACCTG
    AGCTTTGTGG AGGAGGTGTT TGAGAACCAG ACCCGGCTGC CCGGAGGCCA GTGGATCTAC
    ATGAGTGACA ACTACACAGA TGTGAACGGG GAGAAGGTGC TTCCCAAGGA TGACATCGAG
    TGCCCACTGG GCTGGAAGTG GGAAGATGAA GAATGGTCCA CAGACCTCAA TCGGGCCGTT
    GACGAGCAAG GCTGGGAGTA CAGCATCACC ATCCCTCCGG AACGGAAGCC AAGGCACTGG
    GTCCCTGCCG AGAAGATGTA CTACACACAC CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG
    AGGGACCTCA GCCAGATGGA GGCGCTGAAG AGGCACAGGC AGGCGGAGGC GGAGGGCGAG
    GGCTGGGAGT ACGCCTCGCT CTTCGGCTGG AAGTTCCACC TGGAGTACCG CAAGACCGAT
    GCCTTCCGCC GCCGCCGCTG GCGCCGCCGC ATGGAGCCAC TGGAGAAGAC AGGGCCTGCA
    GCTGTGTTTG CCCTCGAGGG GGCCCTGGGC GGCGTGGTGG ACGACAGGAG TGAAGATTCG
    GTGTCCGTCT CCACCTTGAG CTTCGGCGTG AACAGACCCA CGATTTCCTG CATCTTCGAC
    TACGGGAACC GCTACCATCT ACGCTGCTAC ATGTACCAGG CCCGGGACCT GCCCGCCATG
    GACAAGGACT CTTTTTCTGA TCCCTACGCA ATCGTCTCCT TCCTGCACCA GAGCCAGAAG
    ACAGTGGTGG TGAAGAACAC CCTGAACCCC ACCTGGGACC AGACACTCAT CTTCTATGAG
    ATCGAGATCT TTGGTGAGCC GCCCAGCATC GCCGAGCAGC CGCCCAGCAT CGTGGTGGAG
    CTATACGACC ATGACACCTA CGGTGCAGAT GAGTTTATGG GACGCTGCAT CTGTCAGCCG
    ACTCTGGAAC GGATGCCCCG GCTGACCTGG TTCCCACTGA CTAGGGGCAG CCAGCCGGCG
    GGCGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCGGCCAT CTACCACATT
    CCTGGTTTTG AGGTGCAGGA TACATCAGGG ATCCTGGAAG AGTCGGAGGA CACAGACCTG
    CCCTACCCGC CACCCCAGAG AGAGGCCAAC ATCTACATGG TTCCCCAGAA CATCAAGCCT
    GCTCTCCAGC GGACGGCCAT CGAGATCCTG GCATGGGGCC TGCGGAACAT GAAGAGTTAC
    CACCTGGCCA GTGTCTCCTC CCCCAGCCTC GTGGTGGAGT GCGGGGGCCA GACGGTGCAG
    TCCTGTGTCA TCAGGAACCT CCAGAAGAAC CCCAACTTCG ACGTCTGCAC CCTCTTCATG
    GAAGTGATGC TGCCCAGGGA GGAGCTCTAC TGTCCCCCCA TCGTGGTCAA GGTCATCGAT
    AACTGCCAGT TTGGCCGCCG GCCTGTGGTG GGCCAGTGTA CCATCTGCTC CCTGGAGAGC
    TTCCTGTGTG ACCCCTACTC AGAGGAGAGT CCGTCCCCAC AGGGCGGCCC AGATGATGTG
    AGCTTACTCA GTCCTGGGGA AGATGTGCTC ATTGACATTG ATGACAAGGA GCCCCTCATC
    CCTATGCAGG AGGAGGAGTT CATCGATTGG TGGAGCAAAT TCTTTGCCTC GATAGGGGAG
    AGGGAAAAGT GCGGGTCCTA CTTGGAGAAG GACTTTGACA CTCTAAAGGT CTATGACACA
    CAACTGGAGA ATGTGGAGGC CTTTGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTC
    TACCGGGGCA AGACTCAGGA GGAGATGGAA GACCCATCTG TCGTTGGGGA ATTTAAGGGC
    CTCTTCAAAA TTTATCCCCT CCCAGAAGAC CCGGCCATCC CCATGCCCCC AAGACAGTTC
    CACCAGCTAG CTGCCCAGGG ACCCCAGGAG TGCCTGGTCC GCATCTACAT CATCCGGGCA
    TTCGGCCTGC AGCCCAAGGA CCCCAATGGG AAGTGTGACC CTTACATCAA GATCTCCATA
    GGGAAGAAAT CCGTGAGCGA CCAGGATAAC TACATCCCCT GCACTCTGGA GCCTGTGTTT
    GGAAAGATGT TCGAACTGAC CTGCACTCTG CCTCTGGAGA AGGACCTGAA GATCACTCTC
    TATGACTATG ACCTCCTCTC CAAGGACGAG ATGATCGGGG AGACGGTCAT CGACCTGGAG
    AATAGGCTGC TGTCCAAGTT TGGAGCTCGC TGCGGACTCC CACAGACCTA CTGTGTCTCT
    GGACCGAACC AGTGGAGGGA CCAGCTCCGC CCCTCCCAGC TTCTCCACCT GTTCTGCCAG
    CAGCAGAGAG TCAAGGCCCC CGTGTACCAG ACCGACCGTG TGGTGTTTGG GAATAAAGAA
    TACACGATTG AAGAAATAGA GGCTGGGAGG GTCCTGAATC CACACCTGGG CCCAGTGGAA
    GAGCGCCTGG CCTTGCACGT CCTCCAGCAG CAGGGCTTGG TGCCCGAGCA TGTGGAGTCC
    CGGCCCCTCT ATAGCCCCCT GCAGCCGGAC ATTGAGCAGG GGAAGCTGCA GATGTGGATC
    GACCTGTTTC CAAAGGCCCT GGGGCGGCCT GGACCTCCCT TCAACATCAC TCCACGGAGA
    GCCAGAAGGT TTTTCCTGCG TTGCATTATC TGGAACACCA AGGATGTGAT CCTGGACGAC
    CTCAGCATCA CAGGAGAGAA GATGAGCGAC ATTTATGTGA AAGGTTGGAT GGTTGGCTTT
    GAAGAACACA AGCAAAAGAC AGATGTGCAT TATCGGTCCC TGGGAGGCGA AGGGAACTTT
    AACTGGAGGT TCATTTTCCC CTTTGACTAC CTGCTGGCTG AGCAAGTCTG CACCGTCTCC
    AAGAAGGATG CTTTCTGGAA GCTGGACAAG ACCGAGAGCA AAATCCCAGC ACGAGTGGTA
    TTCCAGATCT GGGACAATGA CAAGTTCTCC TTTGATGATT TCCTGGGCTC CTTGCAGCTG
    GATCTCAACC GCATGCCCAA GCCTGCCAAG ACAGCCGAGA AGTGCTCCCT GGACCAGCTG
    GATGACACTT TCCACCCAGA GTGGTTTGTG TCCCTTTTTG AACAGAAAAC TGTGAAGGGC
    TGGTGGCCCT GTGTGGCGGA AGAGGGTGAG AAGAAGAAGC TGGCGGGCAA GCTGGAGATG
    ACCTTGGAGA TTGTGGCGGA GAGTGAGCAT GAGGAGCGGC CCGCTGGCCA GGGCCGGGAT
    GAGCCCAACA TGAACCCCAA GCTCGAGGAC CCAAGGCGCC CTGACACCTC CTTCCTCTGG
    TTCACCTCCC CGTACAAGAC TATGAAGTTC ATCCTGTGGC GGCGTTTCCG GTGTGCCATC
    ATCCTCTTCA TCATCCTCTT CATCCTACTG CTGTTCTTGG GCATCTTCGT TTACGCCTTC
    CCGAACTATG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023474690.1
    CDS524..6865
    Translation

    Target ORF information:

    RefSeq Version XM_023618922.1
    Organism Equus caballus(horse)
    Definition Equus caballus dysferlin (DYSF), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023618922.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGAGGG TCTTTTTCCT TTACGCCGAG AACGTCCACA CCCCGGACAC CGACATCAGC 
    GATGCCTACT GCTCCGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAAAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGGGT CCCCCTGGAC
    CAGAGCTCTG AGCTTCACGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
    CTGGGAGAAG CCAAGGTCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CGCCTCTCCC TGGAGCTGTG CCCCTGTTCC CACCCTCTGT GCCGCTGGAA
    CCCTCCCCGA CTCTGCCCGA TGTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCCGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAATGGCAG
    GCCCCCACGG ATACAGGAGG AGAGGAGGAC ACCGAGGACC AGGGGCTCAC GGGAGATGAG
    GCAGAGCCAT TCTTGGATCA GAACGGAGCC CCAGGCCCCG GGGCTCCCAC CACCCTGAAG
    AAGCCACCTT CCCATCCTCC CCCCTACCAT CCTGGGGGGA AAAGGAAGAG AAGCACGCCT
    GCGCCCAGAA AGCTGCTTTC GGATAAACCA CAGGACTTCC AGATCAGGGT CCAGGTGATC
    GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC CGCGGCCAGG
    CAGACCAAGC GGACTCGGAT TCACAGGGGA AACAGCCCGC TCTTCAACGA GACTCTCTTC
    TTCAACGTGT TTGACTCTCC CTCGGAGCTG TTTGACGAGG CCGTCTTTAT CACGGTGGTA
    GACTCCTGTT CGCTCCGGAC AGATGCCCTC ATCGGGGAGT TCCGGATGGA TGTGGGTACC
    ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTGCTCTC GGACCCTGAC
    GATTTCTCTG CTGGGCCCAA AGGCTACCTG AAAGCAAGCC TTTGTGTGCT GGGGCCTGGA
    GACGAAGCTC CGCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAGCAAT
    CTGCTCAGGC CAACTGGCAT GGCCCTTCGA GGAGCGCACT TCTGTCTGAA GGTCTTCAGG
    GCTGAGGACT TACCACAGAT GGACGATGCC GTGGTGGACA GCGTGAAGCA GATCTTCGGC
    TTTGACAGTA ACAAGAAGAA CCTGGTGGAT CCCTTCGTCG AGGTCAGCTT TGCGGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGATG GCCAACCCTC AGTGGAACCA GAGCATCACG
    CTGCCTGTCA TGTTTCCCTC CATGTCTGAA AAAATGAGGA TTCGTGTCAT AGACTGGGAC
    CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA ATATGTCGAA AATCTCTGCC
    CCTGGAGGAG AAATAGCAGA GGAGCCTGCA GGTGTGGTCA AGCCTCCTCC AGCCACGGAC
    CTGGACGACC ACCTGGGCTT CCTCCCCACC TTCGGGCCCT GCTATGTCAA CCTCTACGGC
    AGCCCCAGGG AGTTCACTGG CTTCCCAGAC CCCTATGCAG AGCTCAACAC GGGCAAGGGA
    GAAGGCGTGG CCTACCGAGG CCGGCTTCTG GTCTCCCTGG AGACCAAACT GGTGGAGCAA
    GGCGAGCAGA AGGTGGAGGA TCTCGCTGCA GATGACATCC TTCGGGTGGA GAAGTACCTC
    CGGAGGCGCA AGTACTCCCT CTTTGCTGCC TTCTACTCGG CCTGCATGCT GCAGGATGTC
    GACGATGCCA TCCAGTTCGA GGTCAGCATC GGAAACTACG GGAACAAGTT TGACACGACC
    TGCCTGCCGC TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGACGG GTGCCACTAC
    TACTACTTAC CCTGGGGCAA CGTGAAGCCT GTGGTGGTGC TGTCATCCTA CTGGGAAGAC
    ATCAGCCACA GAGTCGACAC TCAGAACCAG CTGCTCCGGA TCGCCGACCG GCTGGAAGCC
    GGCCTGGAGC AGGTCCACCT GGCCCTGAAG GCGCGGTGCT CCACCGAGGA TGTGGACTCC
    CTGGTGGCTC AGCTGATGGA TGAGCTCATT GCAGACTGCA GCCAGCCCCT GTGCAACGTC
    CAAGAGACGC CCTCTGCCAC CCACCTGGAC CAGTACCTGT ACCGGCTGCG CACCCGTCAC
    CTGAGCCAGA TCAGTGAGGC GGCCCTGGCC TTGAAGCTCG GCCACAGTGA GCTCCCCGCC
    GCCCTTGAGC AGGCTGAGGA CTGGCTCCTG CGTCTCCGTG CCCTGGCGGA GGAGCCCCAG
    AACAGCCTGC CTGACGTGGT CATCTGGATG CTGCAGGGAG ACAAGCGGGT GGCGTACCAG
    CGGGTGCCCG CCCACGAGGT CCTCTTCTCC CGGCGGGGCG CCAACTACTG TGGCAAGAAT
    TGTGGGAAGC TGCAGACGAT CTTTCTGAAG TATCCAATGG AAGGGGTGCC GGGAGCCCGG
    ATGCCGGTGC AGATACGGGT GAAGCTCTGG TTCGGGCTCT CTGTGGATGA GAAGGAGTTC
    AATCAGTTTG CTGAGGGCAA GCTCTCTGTC TTCGCTGAAA CCTACGAGAA CCAGACCAAG
    CTGGCCCTAG TCGGGAACTG GGGCACAACG GGCCTCACCT ACCCCAAGTT TTCTGACGTC
    ACAGGCAAGA TCAAGCTACC CAAGGACAGC TTCCGCCCCT CGGCCGGCTG GGCCTGGGCT
    GGAGAGTGGT TCGTGTGTCC GGAGAAGACC CTACTCCACG ACACCGATGC GGGCCACCTG
    AGCTTTGTGG AGGAGGTGTT TGAGAACCAG ACCCGGCTGC CCGGAGGCCA GTGGATCTAC
    ATGAGTGACA ACTACACAGA TGTGAACGGG GAGAAGGTGC TTCCCAAGGA TGACATCGAG
    TGCCCACTGG GCTGGAAGTG GGAAGATGAA GAATGGTCCA CAGACCTCAA TCGGGCCGTT
    GACGAGCAAG GCTGGGAGTA CAGCATCACC ATCCCTCCGG AACGGAAGCC AAGGCACTGG
    GTCCCTGCCG AGAAGATGTA CTACACACAC CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG
    AGGGACCTCA GCCAGATGGA GGCGCTGAAG AGGCACAGGC AGGCGGAGGC GGAGGGCGAG
    GGCTGGGAGT ACGCCTCGCT CTTCGGCTGG AAGTTCCACC TGGAGTACCG CAAGACCGAT
    GCCTTCCGCC GCCGCCGCTG GCGCCGCCGC ATGGAGCCAC TGGAGAAGAC AGGGCCTGCA
    GCTGTGTTTG CCCTCGAGGG GGCCCTGGGC GGCGTGGTGG ACGACAGGAG TGAAGATTCG
    GTGTCCGTCT CCACCTTGAG CTTCGGCGTG AACAGACCCA CGATTTCCTG CATCTTCGAC
    TACGGGAACC GCTACCATCT ACGCTGCTAC ATGTACCAGG CCCGGGACCT GCCCGCCATG
    GACAAGGACT CTTTTTCTGA TCCCTACGCA ATCGTCTCCT TCCTGCACCA GAGCCAGAAG
    ACAGTGGTGG TGAAGAACAC CCTGAACCCC ACCTGGGACC AGACACTCAT CTTCTATGAG
    ATCGAGATCT TTGGTGAGCC GCCCAGCATC GCCGAGCAGC CGCCCAGCAT CGTGGTGGAG
    CTATACGACC ATGACACCTA CGGTGCAGAT GAGTTTATGG GACGCTGCAT CTGTCAGCCG
    ACTCTGGAAC GGATGCCCCG GCTGACCTGG TTCCCACTGA CTAGGGGCAG CCAGCCGGCG
    GGCGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCGGCCAT CTACCACATT
    CCTGGTTTTG AGGTGCAGGA TACATCAGGG ATCCTGGAAG AGTCGGAGGA CACAGACCTG
    CCCTACCCGC CACCCCAGAG AGAGGCCAAC ATCTACATGG TTCCCCAGAA CATCAAGCCT
    GCTCTCCAGC GGACGGCCAT CGAGATCCTG GCATGGGGCC TGCGGAACAT GAAGAGTTAC
    CACCTGGCCA GTGTCTCCTC CCCCAGCCTC GTGGTGGAGT GCGGGGGCCA GACGGTGCAG
    TCCTGTGTCA TCAGGAACCT CCAGAAGAAC CCCAACTTCG ACGTCTGCAC CCTCTTCATG
    GAAGTGATGC TGCCCAGGGA GGAGCTCTAC TGTCCCCCCA TCGTGGTCAA GGTCATCGAT
    AACTGCCAGT TTGGCCGCCG GCCTGTGGTG GGCCAGTGTA CCATCTGCTC CCTGGAGAGC
    TTCCTGTGTG ACCCCTACTC AGAGGAGAGT CCGTCCCCAC AGGGCGGCCC AGATGATGTG
    AGCTTACTCA GTCCTGGGGA AGATGTGCTC ATTGACATTG ATGACAAGGA GCCCCTCATC
    CCTATGCAGG AGGAGGAGTT CATCGATTGG TGGAGCAAAT TCTTTGCCTC GATAGGGGAG
    AGGGAAAAGT GCGGGTCCTA CTTGGAGAAG GACTTTGACA CTCTAAAGGT CTATGACACA
    CAACTGGAGA ATGTGGAGGC CTTTGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTC
    TACCGGGGCA AGACTCAGGA GGAGATGGAA GACCCATCTG TCGTTGGGGA ATTTAAGGGC
    CTCTTCAAAA TTTATCCCCT CCCAGAAGAC CCGGCCATCC CCATGCCCCC AAGACAGTTC
    CACCAGCTAG CTGCCCAGGG ACCCCAGGAG TGCCTGGTCC GCATCTACAT CATCCGGGCA
    TTCGGCCTGC AGCCCAAGGA CCCCAATGGG AAGTGTGACC CTTACATCAA GATCTCCATA
    GGGAAGAAAT CCGTGAGCGA CCAGGATAAC TACATCCCCT GCACTCTGGA GCCTGTGTTT
    GGAAAGATGT TCGAACTGAC CTGCACTCTG CCTCTGGAGA AGGACCTGAA GATCACTCTC
    TATGACTATG ACCTCCTCTC CAAGGACGAG ATGATCGGGG AGACGGTCAT CGACCTGGAG
    AATAGGCTGC TGTCCAAGTT TGGAGCTCGC TGCGGACTCC CACAGACCTA CTGTGTCTCT
    GGACCGAACC AGTGGAGGGA CCAGCTCCGC CCCTCCCAGC TTCTCCACCT GTTCTGCCAG
    CAGCAGAGAG TCAAGGCCCC CGTGTACCAG ACCGACCGTG TGGTGTTTGG GAATAAAGAA
    TACACGATTG AAGAAATAGA GGCTGGGAGG GTCCTGAATC CACACCTGGG CCCAGTGGAA
    GAGCGCCTGG CCTTGCACGT CCTCCAGCAG CAGGGCTTGG TGCCCGAGCA TGTGGAGTCC
    CGGCCCCTCT ATAGCCCCCT GCAGCCGGAC ATTGAGCAGG GGAAGCTGCA GATGTGGATC
    GACCTGTTTC CAAAGGCCCT GGGGCGGCCT GGACCTCCCT TCAACATCAC TCCACGGAGA
    GCCAGAAGGT TTTTCCTGCG TTGCATTATC TGGAACACCA AGGATGTGAT CCTGGACGAC
    CTCAGCATCA CAGGAGAGAA GATGAGCGAC ATTTATGTGA AAGGTTGGAT GGTTGGCTTT
    GAAGAACACA AGCAAAAGAC AGATGTGCAT TATCGGTCCC TGGGAGGCGA AGGGAACTTT
    AACTGGAGGT TCATTTTCCC CTTTGACTAC CTGCTGGCTG AGCAAGTCTG CACCGTCTCC
    AAGAAGGATG CTTTCTGGAA GCTGGACAAG ACCGAGAGCA AAATCCCAGC ACGAGTGGTA
    TTCCAGATCT GGGACAATGA CAAGTTCTCC TTTGATGATT TCCTGGGCTC CTTGCAGCTG
    GATCTCAACC GCATGCCCAA GCCTGCCAAG ACAGCCGAGA AGTGCTCCCT GGACCAGCTG
    GATGACACTT TCCACCCAGA GTGGTTTGTG TCCCTTTTTG AACAGAAAAC TGTGAAGGGC
    TGGTGGCCCT GTGTGGCGGA AGAGGGTGAG AAGAAGAAGC TGGCGGGCAA GCTGGAGATG
    ACCTTGGAGA TTGTGGCGGA GAGTGAGCAT GAGGAGCGGC CCGCTGGCCA GGGCCGGGAT
    GAGCCCAACA TGAACCCCAA GCTCGAGGAC CCAAGGCGCC CTGACACCTC CTTCCTCTGG
    TTCACCTCCC CGTACAAGAC TATGAAGTTC ATCCTGTGGC GGCGTTTCCG GTGTGCCATC
    ATCCTCTTCA TCATCCTCTT CATCCTACTG CTGTTCTTGG GCATCTTCGT TTACGCCTTC
    CCGAACTATG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb49791
    Clone ID Related Accession (Same CDS sequence) XM_023618923.1
    Accession Version XM_023618923.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6312bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product dysferlin isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009158.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGAGGG TCTTTTTCCT TTACGCCGAG AACGTCCACA CCCCGGACAC CGACATCAGC 
    GATGCCTACT GCTCCGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAAAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGGGT CCCCCTGGAC
    CAGAGCTCTG AGCTTCACGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
    CTGGGAGAAG CCAAGGTCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CGCCTCTCCC TGGAGCTGTG CCCCTGTTCC CACCCTCTGT GCCGCTGGAA
    CCCTCCCCGA CTCTGCCCGA TGTGGATATG CTGGCTGATA CAGGAGGAGA GGAGGACACC
    GAGGACCAGG GGCTCACGGG AGATGAGGCA GAGCCATTCT TGGATCAGAA CGGAGCCCCA
    GGCCCCGGGG CTCCCACCAC CCTGAAGAAG CCACCTTCCC ATCCTCCCCC CTACCATCCT
    GGGGGGAAAA GGAAGAGAAG CACGCCTGCG CCCAGAAAGC TGCTTTCGGA TAAACCACAG
    GACTTCCAGA TCAGGGTCCA GGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAACATCAAG
    CCTGTGGTCA AGGTCACCGC GGCCAGGCAG ACCAAGCGGA CTCGGATTCA CAGGGGAAAC
    AGCCCGCTCT TCAACGAGAC TCTCTTCTTC AACGTGTTTG ACTCTCCCTC GGAGCTGTTT
    GACGAGGCCG TCTTTATCAC GGTGGTAGAC TCCTGTTCGC TCCGGACAGA TGCCCTCATC
    GGGGAGTTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTCAGG
    AAGTGGCTGC TGCTCTCGGA CCCTGACGAT TTCTCTGCTG GGCCCAAAGG CTACCTGAAA
    GCAAGCCTTT GTGTGCTGGG GCCTGGAGAC GAAGCTCCGC TGGAGAGAAA GGACCCCTCT
    GAAGACAAGG AGGACATTGA AAGCAATCTG CTCAGGCCAA CTGGCATGGC CCTTCGAGGA
    GCGCACTTCT GTCTGAAGGT CTTCAGGGCT GAGGACTTAC CACAGATGGA CGATGCCGTG
    GTGGACAGCG TGAAGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACCT GGTGGATCCC
    TTCGTCGAGG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGATGGCC
    AACCCTCAGT GGAACCAGAG CATCACGCTG CCTGTCATGT TTCCCTCCAT GTCTGAAAAA
    ATGAGGATTC GTGTCATAGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC
    TACCTGAATA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGCAGAGGA GCCTGCAGGT
    GTGGTCAAGC CTCCTCCAGC CACGGACCTG GACGACCACC TGGGCTTCCT CCCCACCTTC
    GGGCCCTGCT ATGTCAACCT CTACGGCAGC CCCAGGGAGT TCACTGGCTT CCCAGACCCC
    TATGCAGAGC TCAACACGGG CAAGGGAGAA GGCGTGGCCT ACCGAGGCCG GCTTCTGGTC
    TCCCTGGAGA CCAAACTGGT GGAGCAAGGC GAGCAGAAGG TGGAGGATCT CGCTGCAGAT
    GACATCCTTC GGGTGGAGAA GTACCTCCGG AGGCGCAAGT ACTCCCTCTT TGCTGCCTTC
    TACTCGGCCT GCATGCTGCA GGATGTCGAC GATGCCATCC AGTTCGAGGT CAGCATCGGA
    AACTACGGGA ACAAGTTTGA CACGACCTGC CTGCCGCTGG CCTCCACCAC CCAGTACAGC
    CGGGCGGTCT TTGACGGGTG CCACTACTAC TACTTACCCT GGGGCAACGT GAAGCCTGTG
    GTGGTGCTGT CATCCTACTG GGAAGACATC AGCCACAGAG TCGACACTCA GAACCAGCTG
    CTCCGGATCG CCGACCGGCT GGAAGCCGGC CTGGAGCAGG TCCACCTGGC CCTGAAGGCG
    CGGTGCTCCA CCGAGGATGT GGACTCCCTG GTGGCTCAGC TGATGGATGA GCTCATTGCA
    GACTGCAGCC AGCCCCTGTG CAACGTCCAA GAGACGCCCT CTGCCACCCA CCTGGACCAG
    TACCTGTACC GGCTGCGCAC CCGTCACCTG AGCCAGATCA GTGAGGCGGC CCTGGCCTTG
    AAGCTCGGCC ACAGTGAGCT CCCCGCCGCC CTTGAGCAGG CTGAGGACTG GCTCCTGCGT
    CTCCGTGCCC TGGCGGAGGA GCCCCAGAAC AGCCTGCCTG ACGTGGTCAT CTGGATGCTG
    CAGGGAGACA AGCGGGTGGC GTACCAGCGG GTGCCCGCCC ACGAGGTCCT CTTCTCCCGG
    CGGGGCGCCA ACTACTGTGG CAAGAATTGT GGGAAGCTGC AGACGATCTT TCTGAAGTAT
    CCAATGGAAG GGGTGCCGGG AGCCCGGATG CCGGTGCAGA TACGGGTGAA GCTCTGGTTC
    GGGCTCTCTG TGGATGAGAA GGAGTTCAAT CAGTTTGCTG AGGGCAAGCT CTCTGTCTTC
    GCTGAAACCT ACGAGAACCA GACCAAGCTG GCCCTAGTCG GGAACTGGGG CACAACGGGC
    CTCACCTACC CCAAGTTTTC TGACGTCACA GGCAAGATCA AGCTACCCAA GGACAGCTTC
    CGCCCCTCGG CCGGCTGGGC CTGGGCTGGA GAGTGGTTCG TGTGTCCGGA GAAGACCCTA
    CTCCACGACA CCGATGCGGG CCACCTGAGC TTTGTGGAGG AGGTGTTTGA GAACCAGACC
    CGGCTGCCCG GAGGCCAGTG GATCTACATG AGTGACAACT ACACAGATGT GAACGGGGAG
    AAGGTGCTTC CCAAGGATGA CATCGAGTGC CCACTGGGCT GGAAGTGGGA AGATGAAGAA
    TGGTCCACAG ACCTCAATCG GGCCGTTGAC GAGCAAGGCT GGGAGTACAG CATCACCATC
    CCTCCGGAAC GGAAGCCAAG GCACTGGGTC CCTGCCGAGA AGATGTACTA CACACACCGT
    CGGCGGCGCT GGGTCCGCCT GCGCAGGAGG GACCTCAGCC AGATGGAGGC GCTGAAGAGG
    CACAGGCAGG CGGAGGCGGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT CGGCTGGAAG
    TTCCACCTGG AGTACCGCAA GACCGATGCC TTCCGCCGCC GCCGCTGGCG CCGCCGCATG
    GAGCCACTGG AGAAGACAGG GCCTGCAGCT GTGTTTGCCC TCGAGGGGGC CCTGGGCGGC
    GTGGTGGACG ACAGGAGTGA AGATTCGGTG TCCGTCTCCA CCTTGAGCTT CGGCGTGAAC
    AGACCCACGA TTTCCTGCAT CTTCGACTAC GGGAACCGCT ACCATCTACG CTGCTACATG
    TACCAGGCCC GGGACCTGCC CGCCATGGAC AAGGACTCTT TTTCTGATCC CTACGCAATC
    GTCTCCTTCC TGCACCAGAG CCAGAAGACA GTGGTGGTGA AGAACACCCT GAACCCCACC
    TGGGACCAGA CACTCATCTT CTATGAGATC GAGATCTTTG GTGAGCCGCC CAGCATCGCC
    GAGCAGCCGC CCAGCATCGT GGTGGAGCTA TACGACCATG ACACCTACGG TGCAGATGAG
    TTTATGGGAC GCTGCATCTG TCAGCCGACT CTGGAACGGA TGCCCCGGCT GACCTGGTTC
    CCACTGACTA GGGGCAGCCA GCCGGCGGGC GAGCTGCTGG CCTCTTTTGA GCTCATCCAG
    AGAGAGAAGC CGGCCATCTA CCACATTCCT GGTTTTGAGG TGCAGGATAC ATCAGGGATC
    CTGGAAGAGT CGGAGGACAC AGACCTGCCC TACCCGCCAC CCCAGAGAGA GGCCAACATC
    TACATGGTTC CCCAGAACAT CAAGCCTGCT CTCCAGCGGA CGGCCATCGA GATCCTGGCA
    TGGGGCCTGC GGAACATGAA GAGTTACCAC CTGGCCAGTG TCTCCTCCCC CAGCCTCGTG
    GTGGAGTGCG GGGGCCAGAC GGTGCAGTCC TGTGTCATCA GGAACCTCCA GAAGAACCCC
    AACTTCGACG TCTGCACCCT CTTCATGGAA GTGATGCTGC CCAGGGAGGA GCTCTACTGT
    CCCCCCATCG TGGTCAAGGT CATCGATAAC TGCCAGTTTG GCCGCCGGCC TGTGGTGGGC
    CAGTGTACCA TCTGCTCCCT GGAGAGCTTC CTGTGTGACC CCTACTCAGA GGAGAGTCCG
    TCCCCACAGG GCGGCCCAGA TGATGTGAGC TTACTCAGTC CTGGGGAAGA TGTGCTCATT
    GACATTGATG ACAAGGAGCC CCTCATCCCT ATGCAGCTTG CAGATGGTCT GTCGGGCTCG
    GCCCCCACTA ACATGGCATC TTCTCCGTCC AGTCCTCATG AGGAGGAGTT CATCGATTGG
    TGGAGCAAAT TCTTTGCCTC GATAGGGGAG AGGGAAAAGT GCGGGTCCTA CTTGGAGAAG
    GACTTTGACA CTCTAAAGGT CTATGACACA CAACTGGAGA ATGTGGAGGC CTTTGAGGGC
    CTGTCTGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA AGACTCAGGA GGAGATGGAA
    GACCCATCTG TCGTTGGGGA ATTTAAGGGC CTCTTCAAAA TTTATCCCCT CCCAGAAGAC
    CCGGCCATCC CCATGCCCCC AAGACAGTTC CACCAGCTAG CTGCCCAGGG ACCCCAGGAG
    TGCCTGGTCC GCATCTACAT CATCCGGGCA TTCGGCCTGC AGCCCAAGGA CCCCAATGGG
    AAGTGTGACC CTTACATCAA GATCTCCATA GGGAAGAAAT CCGTGAGCGA CCAGGATAAC
    TACATCCCCT GCACTCTGGA GCCTGTGTTT GGAAAGATGT TCGAACTGAC CTGCACTCTG
    CCTCTGGAGA AGGACCTGAA GATCACTCTC TATGACTATG ACCTCCTCTC CAAGGACGAG
    ATGATCGGGG AGACGGTCAT CGACCTGGAG AATAGGCTGC TGTCCAAGTT TGGAGCTCGC
    TGCGGACTCC CACAGACCTA CTGTGTCTCT GGACCGAACC AGTGGAGGGA CCAGCTCCGC
    CCCTCCCAGC TTCTCCACCT GTTCTGCCAG CAGCAGAGAG TCAAGGCCCC CGTGTACCAG
    ACCGACCGTG TGGTGTTTGG GAATAAAGAA TACACGATTG AAGAAATAGA GGCTGGGAGG
    GTCCTGAATC CACACCTGGG CCCAGTGGAA GAGCGCCTGG CCTTGCACGT CCTCCAGCAG
    CAGGGCTTGG TGCCCGAGCA TGTGGAGTCC CGGCCCCTCT ATAGCCCCCT GCAGCCGGAC
    ATTGAGCAGG GGAAGCTGCA GATGTGGATC GACCTGTTTC CAAAGGCCCT GGGGCGGCCT
    GGACCTCCCT TCAACATCAC TCCACGGAGA GCCAGAAGGT TTTTCCTGCG TTGCATTATC
    TGGAACACCA AGGATGTGAT CCTGGACGAC CTCAGCATCA CAGGAGAGAA GATGAGCGAC
    ATTTATGTGA AAGGTTGGAT GGTTGGCTTT GAAGAACACA AGCAAAAGAC AGATGTGCAT
    TATCGGTCCC TGGGAGGCGA AGGGAACTTT AACTGGAGGT TCATTTTCCC CTTTGACTAC
    CTGCTGGCTG AGCAAGTCTG CACCGTCTCC AAGAAGGATG CTTTCTGGAA GCTGGACAAG
    ACCGAGAGCA AAATCCCAGC ACGAGTGGTA TTCCAGATCT GGGACAATGA CAAGTTCTCC
    TTTGATGATT TCCTGGGCTC CTTGCAGCTG GATCTCAACC GCATGCCCAA GCCTGCCAAG
    ACAGCCGAGA AGTGCTCCCT GGACCAGCTG GATGACACTT TCCACCCAGA GTGGTTTGTG
    TCCCTTTTTG AACAGAAAAC TGTGAAGGGC TGGTGGCCCT GTGTGGCGGA AGAGGGTGAG
    AAGAAGAAGC TGGCGGGCAA GCTGGAGATG ACCTTGGAGA TTGTGGCGGA GAGTGAGCAT
    GAGGAGCGGC CCGCTGGCCA GGGCCGGGAT GAGCCCAACA TGAACCCCAA GCTCGAGGAC
    CCAAGGCGCC CTGACACCTC CTTCCTCTGG TTCACCTCCC CGTACAAGAC TATGAAGTTC
    ATCCTGTGGC GGCGTTTCCG GTGTGCCATC ATCCTCTTCA TCATCCTCTT CATCCTACTG
    CTGTTCTTGG GCATCTTCGT TTACGCCTTC CCGAACTATG CTGCCATGAA GCTGGTGAAG
    CCCTTCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023474691.1
    CDS524..6835
    Translation

    Target ORF information:

    RefSeq Version XM_023618923.1
    Organism Equus caballus(horse)
    Definition Equus caballus dysferlin (DYSF), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023618923.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGAGGG TCTTTTTCCT TTACGCCGAG AACGTCCACA CCCCGGACAC CGACATCAGC 
    GATGCCTACT GCTCCGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAAAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGGGT CCCCCTGGAC
    CAGAGCTCTG AGCTTCACGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
    CTGGGAGAAG CCAAGGTCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CGCCTCTCCC TGGAGCTGTG CCCCTGTTCC CACCCTCTGT GCCGCTGGAA
    CCCTCCCCGA CTCTGCCCGA TGTGGATATG CTGGCTGATA CAGGAGGAGA GGAGGACACC
    GAGGACCAGG GGCTCACGGG AGATGAGGCA GAGCCATTCT TGGATCAGAA CGGAGCCCCA
    GGCCCCGGGG CTCCCACCAC CCTGAAGAAG CCACCTTCCC ATCCTCCCCC CTACCATCCT
    GGGGGGAAAA GGAAGAGAAG CACGCCTGCG CCCAGAAAGC TGCTTTCGGA TAAACCACAG
    GACTTCCAGA TCAGGGTCCA GGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAACATCAAG
    CCTGTGGTCA AGGTCACCGC GGCCAGGCAG ACCAAGCGGA CTCGGATTCA CAGGGGAAAC
    AGCCCGCTCT TCAACGAGAC TCTCTTCTTC AACGTGTTTG ACTCTCCCTC GGAGCTGTTT
    GACGAGGCCG TCTTTATCAC GGTGGTAGAC TCCTGTTCGC TCCGGACAGA TGCCCTCATC
    GGGGAGTTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTCAGG
    AAGTGGCTGC TGCTCTCGGA CCCTGACGAT TTCTCTGCTG GGCCCAAAGG CTACCTGAAA
    GCAAGCCTTT GTGTGCTGGG GCCTGGAGAC GAAGCTCCGC TGGAGAGAAA GGACCCCTCT
    GAAGACAAGG AGGACATTGA AAGCAATCTG CTCAGGCCAA CTGGCATGGC CCTTCGAGGA
    GCGCACTTCT GTCTGAAGGT CTTCAGGGCT GAGGACTTAC CACAGATGGA CGATGCCGTG
    GTGGACAGCG TGAAGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACCT GGTGGATCCC
    TTCGTCGAGG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGATGGCC
    AACCCTCAGT GGAACCAGAG CATCACGCTG CCTGTCATGT TTCCCTCCAT GTCTGAAAAA
    ATGAGGATTC GTGTCATAGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC
    TACCTGAATA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGCAGAGGA GCCTGCAGGT
    GTGGTCAAGC CTCCTCCAGC CACGGACCTG GACGACCACC TGGGCTTCCT CCCCACCTTC
    GGGCCCTGCT ATGTCAACCT CTACGGCAGC CCCAGGGAGT TCACTGGCTT CCCAGACCCC
    TATGCAGAGC TCAACACGGG CAAGGGAGAA GGCGTGGCCT ACCGAGGCCG GCTTCTGGTC
    TCCCTGGAGA CCAAACTGGT GGAGCAAGGC GAGCAGAAGG TGGAGGATCT CGCTGCAGAT
    GACATCCTTC GGGTGGAGAA GTACCTCCGG AGGCGCAAGT ACTCCCTCTT TGCTGCCTTC
    TACTCGGCCT GCATGCTGCA GGATGTCGAC GATGCCATCC AGTTCGAGGT CAGCATCGGA
    AACTACGGGA ACAAGTTTGA CACGACCTGC CTGCCGCTGG CCTCCACCAC CCAGTACAGC
    CGGGCGGTCT TTGACGGGTG CCACTACTAC TACTTACCCT GGGGCAACGT GAAGCCTGTG
    GTGGTGCTGT CATCCTACTG GGAAGACATC AGCCACAGAG TCGACACTCA GAACCAGCTG
    CTCCGGATCG CCGACCGGCT GGAAGCCGGC CTGGAGCAGG TCCACCTGGC CCTGAAGGCG
    CGGTGCTCCA CCGAGGATGT GGACTCCCTG GTGGCTCAGC TGATGGATGA GCTCATTGCA
    GACTGCAGCC AGCCCCTGTG CAACGTCCAA GAGACGCCCT CTGCCACCCA CCTGGACCAG
    TACCTGTACC GGCTGCGCAC CCGTCACCTG AGCCAGATCA GTGAGGCGGC CCTGGCCTTG
    AAGCTCGGCC ACAGTGAGCT CCCCGCCGCC CTTGAGCAGG CTGAGGACTG GCTCCTGCGT
    CTCCGTGCCC TGGCGGAGGA GCCCCAGAAC AGCCTGCCTG ACGTGGTCAT CTGGATGCTG
    CAGGGAGACA AGCGGGTGGC GTACCAGCGG GTGCCCGCCC ACGAGGTCCT CTTCTCCCGG
    CGGGGCGCCA ACTACTGTGG CAAGAATTGT GGGAAGCTGC AGACGATCTT TCTGAAGTAT
    CCAATGGAAG GGGTGCCGGG AGCCCGGATG CCGGTGCAGA TACGGGTGAA GCTCTGGTTC
    GGGCTCTCTG TGGATGAGAA GGAGTTCAAT CAGTTTGCTG AGGGCAAGCT CTCTGTCTTC
    GCTGAAACCT ACGAGAACCA GACCAAGCTG GCCCTAGTCG GGAACTGGGG CACAACGGGC
    CTCACCTACC CCAAGTTTTC TGACGTCACA GGCAAGATCA AGCTACCCAA GGACAGCTTC
    CGCCCCTCGG CCGGCTGGGC CTGGGCTGGA GAGTGGTTCG TGTGTCCGGA GAAGACCCTA
    CTCCACGACA CCGATGCGGG CCACCTGAGC TTTGTGGAGG AGGTGTTTGA GAACCAGACC
    CGGCTGCCCG GAGGCCAGTG GATCTACATG AGTGACAACT ACACAGATGT GAACGGGGAG
    AAGGTGCTTC CCAAGGATGA CATCGAGTGC CCACTGGGCT GGAAGTGGGA AGATGAAGAA
    TGGTCCACAG ACCTCAATCG GGCCGTTGAC GAGCAAGGCT GGGAGTACAG CATCACCATC
    CCTCCGGAAC GGAAGCCAAG GCACTGGGTC CCTGCCGAGA AGATGTACTA CACACACCGT
    CGGCGGCGCT GGGTCCGCCT GCGCAGGAGG GACCTCAGCC AGATGGAGGC GCTGAAGAGG
    CACAGGCAGG CGGAGGCGGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT CGGCTGGAAG
    TTCCACCTGG AGTACCGCAA GACCGATGCC TTCCGCCGCC GCCGCTGGCG CCGCCGCATG
    GAGCCACTGG AGAAGACAGG GCCTGCAGCT GTGTTTGCCC TCGAGGGGGC CCTGGGCGGC
    GTGGTGGACG ACAGGAGTGA AGATTCGGTG TCCGTCTCCA CCTTGAGCTT CGGCGTGAAC
    AGACCCACGA TTTCCTGCAT CTTCGACTAC GGGAACCGCT ACCATCTACG CTGCTACATG
    TACCAGGCCC GGGACCTGCC CGCCATGGAC AAGGACTCTT TTTCTGATCC CTACGCAATC
    GTCTCCTTCC TGCACCAGAG CCAGAAGACA GTGGTGGTGA AGAACACCCT GAACCCCACC
    TGGGACCAGA CACTCATCTT CTATGAGATC GAGATCTTTG GTGAGCCGCC CAGCATCGCC
    GAGCAGCCGC CCAGCATCGT GGTGGAGCTA TACGACCATG ACACCTACGG TGCAGATGAG
    TTTATGGGAC GCTGCATCTG TCAGCCGACT CTGGAACGGA TGCCCCGGCT GACCTGGTTC
    CCACTGACTA GGGGCAGCCA GCCGGCGGGC GAGCTGCTGG CCTCTTTTGA GCTCATCCAG
    AGAGAGAAGC CGGCCATCTA CCACATTCCT GGTTTTGAGG TGCAGGATAC ATCAGGGATC
    CTGGAAGAGT CGGAGGACAC AGACCTGCCC TACCCGCCAC CCCAGAGAGA GGCCAACATC
    TACATGGTTC CCCAGAACAT CAAGCCTGCT CTCCAGCGGA CGGCCATCGA GATCCTGGCA
    TGGGGCCTGC GGAACATGAA GAGTTACCAC CTGGCCAGTG TCTCCTCCCC CAGCCTCGTG
    GTGGAGTGCG GGGGCCAGAC GGTGCAGTCC TGTGTCATCA GGAACCTCCA GAAGAACCCC
    AACTTCGACG TCTGCACCCT CTTCATGGAA GTGATGCTGC CCAGGGAGGA GCTCTACTGT
    CCCCCCATCG TGGTCAAGGT CATCGATAAC TGCCAGTTTG GCCGCCGGCC TGTGGTGGGC
    CAGTGTACCA TCTGCTCCCT GGAGAGCTTC CTGTGTGACC CCTACTCAGA GGAGAGTCCG
    TCCCCACAGG GCGGCCCAGA TGATGTGAGC TTACTCAGTC CTGGGGAAGA TGTGCTCATT
    GACATTGATG ACAAGGAGCC CCTCATCCCT ATGCAGCTTG CAGATGGTCT GTCGGGCTCG
    GCCCCCACTA ACATGGCATC TTCTCCGTCC AGTCCTCATG AGGAGGAGTT CATCGATTGG
    TGGAGCAAAT TCTTTGCCTC GATAGGGGAG AGGGAAAAGT GCGGGTCCTA CTTGGAGAAG
    GACTTTGACA CTCTAAAGGT CTATGACACA CAACTGGAGA ATGTGGAGGC CTTTGAGGGC
    CTGTCTGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA AGACTCAGGA GGAGATGGAA
    GACCCATCTG TCGTTGGGGA ATTTAAGGGC CTCTTCAAAA TTTATCCCCT CCCAGAAGAC
    CCGGCCATCC CCATGCCCCC AAGACAGTTC CACCAGCTAG CTGCCCAGGG ACCCCAGGAG
    TGCCTGGTCC GCATCTACAT CATCCGGGCA TTCGGCCTGC AGCCCAAGGA CCCCAATGGG
    AAGTGTGACC CTTACATCAA GATCTCCATA GGGAAGAAAT CCGTGAGCGA CCAGGATAAC
    TACATCCCCT GCACTCTGGA GCCTGTGTTT GGAAAGATGT TCGAACTGAC CTGCACTCTG
    CCTCTGGAGA AGGACCTGAA GATCACTCTC TATGACTATG ACCTCCTCTC CAAGGACGAG
    ATGATCGGGG AGACGGTCAT CGACCTGGAG AATAGGCTGC TGTCCAAGTT TGGAGCTCGC
    TGCGGACTCC CACAGACCTA CTGTGTCTCT GGACCGAACC AGTGGAGGGA CCAGCTCCGC
    CCCTCCCAGC TTCTCCACCT GTTCTGCCAG CAGCAGAGAG TCAAGGCCCC CGTGTACCAG
    ACCGACCGTG TGGTGTTTGG GAATAAAGAA TACACGATTG AAGAAATAGA GGCTGGGAGG
    GTCCTGAATC CACACCTGGG CCCAGTGGAA GAGCGCCTGG CCTTGCACGT CCTCCAGCAG
    CAGGGCTTGG TGCCCGAGCA TGTGGAGTCC CGGCCCCTCT ATAGCCCCCT GCAGCCGGAC
    ATTGAGCAGG GGAAGCTGCA GATGTGGATC GACCTGTTTC CAAAGGCCCT GGGGCGGCCT
    GGACCTCCCT TCAACATCAC TCCACGGAGA GCCAGAAGGT TTTTCCTGCG TTGCATTATC
    TGGAACACCA AGGATGTGAT CCTGGACGAC CTCAGCATCA CAGGAGAGAA GATGAGCGAC
    ATTTATGTGA AAGGTTGGAT GGTTGGCTTT GAAGAACACA AGCAAAAGAC AGATGTGCAT
    TATCGGTCCC TGGGAGGCGA AGGGAACTTT AACTGGAGGT TCATTTTCCC CTTTGACTAC
    CTGCTGGCTG AGCAAGTCTG CACCGTCTCC AAGAAGGATG CTTTCTGGAA GCTGGACAAG
    ACCGAGAGCA AAATCCCAGC ACGAGTGGTA TTCCAGATCT GGGACAATGA CAAGTTCTCC
    TTTGATGATT TCCTGGGCTC CTTGCAGCTG GATCTCAACC GCATGCCCAA GCCTGCCAAG
    ACAGCCGAGA AGTGCTCCCT GGACCAGCTG GATGACACTT TCCACCCAGA GTGGTTTGTG
    TCCCTTTTTG AACAGAAAAC TGTGAAGGGC TGGTGGCCCT GTGTGGCGGA AGAGGGTGAG
    AAGAAGAAGC TGGCGGGCAA GCTGGAGATG ACCTTGGAGA TTGTGGCGGA GAGTGAGCAT
    GAGGAGCGGC CCGCTGGCCA GGGCCGGGAT GAGCCCAACA TGAACCCCAA GCTCGAGGAC
    CCAAGGCGCC CTGACACCTC CTTCCTCTGG TTCACCTCCC CGTACAAGAC TATGAAGTTC
    ATCCTGTGGC GGCGTTTCCG GTGTGCCATC ATCCTCTTCA TCATCCTCTT CATCCTACTG
    CTGTTCTTGG GCATCTTCGT TTACGCCTTC CCGAACTATG CTGCCATGAA GCTGGTGAAG
    CCCTTCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb49792
    Clone ID Related Accession (Same CDS sequence) XM_023618924.1
    Accession Version XM_023618924.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6300bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product dysferlin isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009158.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGAGGG TCTTTTTCCT TTACGCCGAG AACGTCCACA CCCCGGACAC CGACATCAGC 
    GATGCCTACT GCTCCGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAAAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGGGT CCCCCTGGAC
    CAGAGCTCTG AGCTTCACGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
    CTGGGAGAAG CCAAGGTCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CGCCTCTCCC TGGAGCTGTG CCCCTGTTCC CACCCTCTGT GCCGCTGGAA
    CCCTCCCCGA CTCTGCCCGA TGTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCCGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAATGGCAG
    GCCCCCACGG ATACAGGAGG AGAGGAGGAC ACCGAGGACC AGGGGCTCAC GGGAGATGAG
    GCAGAGCCAT TCTTGGATCA GAACGGAGCC CCAGGCCCCG GGGCTCCCAC CACCCTGAAG
    AAGCCACCTT CCCATCCTCC CCCCTACCAT CCTGGGGGGA AAAGGAAGAG AAGCACGCCT
    GCGCCCAGAA AGCTGCTTTC GGATAAACCA CAGGACTTCC AGATCAGGGT CCAGGTGATC
    GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC CGCGGCCAGG
    CAGACCAAGC GGACTCGGAT TCACAGGGGA AACAGCCCGC TCTTCAACGA GACTCTCTTC
    TTCAACGTGT TTGACTCTCC CTCGGAGCTG TTTGACGAGG CCGTCTTTAT CACGGTGGTA
    GACTCCTGTT CGCTCCGGAC AGATGCCCTC ATCGGGGAGT TCCGGATGGA TGTGGGTACC
    ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTGCTCTC GGACCCTGAC
    GATTTCTCTG CTGGGCCCAA AGGCTACCTG AAAGCAAGCC TTTGTGTGCT GGGGCCTGGA
    GACGAAGCTC CGCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAGCAAT
    CTGCTCAGGC CAACTGGCAT GGCCCTTCGA GGAGCGCACT TCTGTCTGAA GGTCTTCAGG
    GCTGAGGACT TACCACAGAT GGACGATGCC GTGGTGGACA GCGTGAAGCA GATCTTCGGC
    TTTGACAGTA ACAAGAAGAA CCTGGTGGAT CCCTTCGTCG AGGTCAGCTT TGCGGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGATG GCCAACCCTC AGTGGAACCA GAGCATCACG
    CTGCCTGTCA TGTTTCCCTC CATGTCTGAA AAAATGAGGA TTCGTGTCAT AGACTGGGAC
    CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA ATATGTCGAA AATCTCTGCC
    CCTGGAGGAG AAATAGCAGT GGACGACCAC CTGGGCTTCC TCCCCACCTT CGGGCCCTGC
    TATGTCAACC TCTACGGCAG CCCCAGGGAG TTCACTGGCT TCCCAGACCC CTATGCAGAG
    CTCAACACGG GCAAGGGAGA AGGCGTGGCC TACCGAGGCC GGCTTCTGGT CTCCCTGGAG
    ACCAAACTGG TGGAGCAAGG CGAGCAGAAG GTGGAGGATC TCGCTGCAGA TGACATCCTT
    CGGGTGGAGA AGTACCTCCG GAGGCGCAAG TACTCCCTCT TTGCTGCCTT CTACTCGGCC
    TGCATGCTGC AGGATGTCGA CGATGCCATC CAGTTCGAGG TCAGCATCGG AAACTACGGG
    AACAAGTTTG ACACGACCTG CCTGCCGCTG GCCTCCACCA CCCAGTACAG CCGGGCGGTC
    TTTGACGGGT GCCACTACTA CTACTTACCC TGGGGCAACG TGAAGCCTGT GGTGGTGCTG
    TCATCCTACT GGGAAGACAT CAGCCACAGA GTCGACACTC AGAACCAGCT GCTCCGGATC
    GCCGACCGGC TGGAAGCCGG CCTGGAGCAG GTCCACCTGG CCCTGAAGGC GCGGTGCTCC
    ACCGAGGATG TGGACTCCCT GGTGGCTCAG CTGATGGATG AGCTCATTGC AGACTGCAGC
    CAGCCCCTGT GCAACGTCCA AGAGACGCCC TCTGCCACCC ACCTGGACCA GTACCTGTAC
    CGGCTGCGCA CCCGTCACCT GAGCCAGATC AGTGAGGCGG CCCTGGCCTT GAAGCTCGGC
    CACAGTGAGC TCCCCGCCGC CCTTGAGCAG GCTGAGGACT GGCTCCTGCG TCTCCGTGCC
    CTGGCGGAGG AGCCCCAGAA CAGCCTGCCT GACGTGGTCA TCTGGATGCT GCAGGGAGAC
    AAGCGGGTGG CGTACCAGCG GGTGCCCGCC CACGAGGTCC TCTTCTCCCG GCGGGGCGCC
    AACTACTGTG GCAAGAATTG TGGGAAGCTG CAGACGATCT TTCTGAAGTA TCCAATGGAA
    GGGGTGCCGG GAGCCCGGAT GCCGGTGCAG ATACGGGTGA AGCTCTGGTT CGGGCTCTCT
    GTGGATGAGA AGGAGTTCAA TCAGTTTGCT GAGGGCAAGC TCTCTGTCTT CGCTGAAACC
    TACGAGAACC AGACCAAGCT GGCCCTAGTC GGGAACTGGG GCACAACGGG CCTCACCTAC
    CCCAAGTTTT CTGACGTCAC AGGCAAGATC AAGCTACCCA AGGACAGCTT CCGCCCCTCG
    GCCGGCTGGG CCTGGGCTGG AGAGTGGTTC GTGTGTCCGG AGAAGACCCT ACTCCACGAC
    ACCGATGCGG GCCACCTGAG CTTTGTGGAG GAGGTGTTTG AGAACCAGAC CCGGCTGCCC
    GGAGGCCAGT GGATCTACAT GAGTGACAAC TACACAGATG TGAACGGGGA GAAGGTGCTT
    CCCAAGGATG ACATCGAGTG CCCACTGGGC TGGAAGTGGG AAGATGAAGA ATGGTCCACA
    GACCTCAATC GGGCCGTTGA CGAGCAAGGC TGGGAGTACA GCATCACCAT CCCTCCGGAA
    CGGAAGCCAA GGCACTGGGT CCCTGCCGAG AAGATGTACT ACACACACCG TCGGCGGCGC
    TGGGTCCGCC TGCGCAGGAG GGACCTCAGC CAGATGGAGG CGCTGAAGAG GCACAGGCAG
    GCGGAGGCGG AGGGCGAGGG CTGGGAGTAC GCCTCGCTCT TCGGCTGGAA GTTCCACCTG
    GAGTACCGCA AGACCGATGC CTTCCGCCGC CGCCGCTGGC GCCGCCGCAT GGAGCCACTG
    GAGAAGACAG GGCCTGCAGC TGTGTTTGCC CTCGAGGGGG CCCTGGGCGG CGTGGTGGAC
    GACAGGAGTG AAGATTCGGT GTCCGTCTCC ACCTTGAGCT TCGGCGTGAA CAGACCCACG
    ATTTCCTGCA TCTTCGACTA CGGGAACCGC TACCATCTAC GCTGCTACAT GTACCAGGCC
    CGGGACCTGC CCGCCATGGA CAAGGACTCT TTTTCTGATC CCTACGCAAT CGTCTCCTTC
    CTGCACCAGA GCCAGAAGAC AGTGGTGGTG AAGAACACCC TGAACCCCAC CTGGGACCAG
    ACACTCATCT TCTATGAGAT CGAGATCTTT GGTGAGCCGC CCAGCATCGC CGAGCAGCCG
    CCCAGCATCG TGGTGGAGCT ATACGACCAT GACACCTACG GTGCAGATGA GTTTATGGGA
    CGCTGCATCT GTCAGCCGAC TCTGGAACGG ATGCCCCGGC TGACCTGGTT CCCACTGACT
    AGGGGCAGCC AGCCGGCGGG CGAGCTGCTG GCCTCTTTTG AGCTCATCCA GAGAGAGAAG
    CCGGCCATCT ACCACATTCC TGGTTTTGAG GTGCAGGATA CATCAGGGAT CCTGGAAGAG
    TCGGAGGACA CAGACCTGCC CTACCCGCCA CCCCAGAGAG AGGCCAACAT CTACATGGTT
    CCCCAGAACA TCAAGCCTGC TCTCCAGCGG ACGGCCATCG AGATCCTGGC ATGGGGCCTG
    CGGAACATGA AGAGTTACCA CCTGGCCAGT GTCTCCTCCC CCAGCCTCGT GGTGGAGTGC
    GGGGGCCAGA CGGTGCAGTC CTGTGTCATC AGGAACCTCC AGAAGAACCC CAACTTCGAC
    GTCTGCACCC TCTTCATGGA AGTGATGCTG CCCAGGGAGG AGCTCTACTG TCCCCCCATC
    GTGGTCAAGG TCATCGATAA CTGCCAGTTT GGCCGCCGGC CTGTGGTGGG CCAGTGTACC
    ATCTGCTCCC TGGAGAGCTT CCTGTGTGAC CCCTACTCAG AGGAGAGTCC GTCCCCACAG
    GGCGGCCCAG ATGATGTGAG CTTACTCAGT CCTGGGGAAG ATGTGCTCAT TGACATTGAT
    GACAAGGAGC CCCTCATCCC TATGCAGGAG GAGGAGTTCA TCGATTGGTG GAGCAAATTC
    TTTGCCTCGA TAGGGGAGAG GGAAAAGTGC GGGTCCTACT TGGAGAAGGA CTTTGACACT
    CTAAAGGTCT ATGACACACA ACTGGAGAAT GTGGAGGCCT TTGAGGGCCT GTCTGACTTT
    TGTAACACCT TCAAGCTCTA CCGGGGCAAG ACTCAGGAGG AGATGGAAGA CCCATCTGTC
    GTTGGGGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC GGCCATCCCC
    ATGCCCCCAA GACAGTTCCA CCAGCTAGCT GCCCAGGGAC CCCAGGAGTG CCTGGTCCGC
    ATCTACATCA TCCGGGCATT CGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGACCCT
    TACATCAAGA TCTCCATAGG GAAGAAATCC GTGAGCGACC AGGATAACTA CATCCCCTGC
    ACTCTGGAGC CTGTGTTTGG AAAGATGTTC GAACTGACCT GCACTCTGCC TCTGGAGAAG
    GACCTGAAGA TCACTCTCTA TGACTATGAC CTCCTCTCCA AGGACGAGAT GATCGGGGAG
    ACGGTCATCG ACCTGGAGAA TAGGCTGCTG TCCAAGTTTG GAGCTCGCTG CGGACTCCCA
    CAGACCTACT GTGTCTCTGG ACCGAACCAG TGGAGGGACC AGCTCCGCCC CTCCCAGCTT
    CTCCACCTGT TCTGCCAGCA GCAGAGAGTC AAGGCCCCCG TGTACCAGAC CGACCGTGTG
    GTGTTTGGGA ATAAAGAATA CACGATTGAA GAAATAGAGG CTGGGAGGGT CCTGAATCCA
    CACCTGGGCC CAGTGGAAGA GCGCCTGGCC TTGCACGTCC TCCAGCAGCA GGGCTTGGTG
    CCCGAGCATG TGGAGTCCCG GCCCCTCTAT AGCCCCCTGC AGCCGGACAT TGAGCAGGGG
    AAGCTGCAGA TGTGGATCGA CCTGTTTCCA AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC
    AACATCACTC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT GCATTATCTG GAACACCAAG
    GATGTGATCC TGGACGACCT CAGCATCACA GGAGAGAAGA TGAGCGACAT TTATGTGAAA
    GGTTGGATGG TTGGCTTTGA AGAACACAAG CAAAAGACAG ATGTGCATTA TCGGTCCCTG
    GGAGGCGAAG GGAACTTTAA CTGGAGGTTC ATTTTCCCCT TTGACTACCT GCTGGCTGAG
    CAAGTCTGCA CCGTCTCCAA GAAGGATGCT TTCTGGAAGC TGGACAAGAC CGAGAGCAAA
    ATCCCAGCAC GAGTGGTATT CCAGATCTGG GACAATGACA AGTTCTCCTT TGATGATTTC
    CTGGGCTCCT TGCAGCTGGA TCTCAACCGC ATGCCCAAGC CTGCCAAGAC AGCCGAGAAG
    TGCTCCCTGG ACCAGCTGGA TGACACTTTC CACCCAGAGT GGTTTGTGTC CCTTTTTGAA
    CAGAAAACTG TGAAGGGCTG GTGGCCCTGT GTGGCGGAAG AGGGTGAGAA GAAGAAGCTG
    GCGGGCAAGC TGGAGATGAC CTTGGAGATT GTGGCGGAGA GTGAGCATGA GGAGCGGCCC
    GCTGGCCAGG GCCGGGATGA GCCCAACATG AACCCCAAGC TCGAGGACCC AAGGCGCCCT
    GACACCTCCT TCCTCTGGTT CACCTCCCCG TACAAGACTA TGAAGTTCAT CCTGTGGCGG
    CGTTTCCGGT GTGCCATCAT CCTCTTCATC ATCCTCTTCA TCCTACTGCT GTTCTTGGGC
    ATCTTCGTTT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023474692.1
    CDS524..6823
    Translation

    Target ORF information:

    RefSeq Version XM_023618924.1
    Organism Equus caballus(horse)
    Definition Equus caballus dysferlin (DYSF), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023618924.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGAGGG TCTTTTTCCT TTACGCCGAG AACGTCCACA CCCCGGACAC CGACATCAGC 
    GATGCCTACT GCTCCGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAAAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGGGT CCCCCTGGAC
    CAGAGCTCTG AGCTTCACGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
    CTGGGAGAAG CCAAGGTCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CGCCTCTCCC TGGAGCTGTG CCCCTGTTCC CACCCTCTGT GCCGCTGGAA
    CCCTCCCCGA CTCTGCCCGA TGTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCCGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAATGGCAG
    GCCCCCACGG ATACAGGAGG AGAGGAGGAC ACCGAGGACC AGGGGCTCAC GGGAGATGAG
    GCAGAGCCAT TCTTGGATCA GAACGGAGCC CCAGGCCCCG GGGCTCCCAC CACCCTGAAG
    AAGCCACCTT CCCATCCTCC CCCCTACCAT CCTGGGGGGA AAAGGAAGAG AAGCACGCCT
    GCGCCCAGAA AGCTGCTTTC GGATAAACCA CAGGACTTCC AGATCAGGGT CCAGGTGATC
    GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC CGCGGCCAGG
    CAGACCAAGC GGACTCGGAT TCACAGGGGA AACAGCCCGC TCTTCAACGA GACTCTCTTC
    TTCAACGTGT TTGACTCTCC CTCGGAGCTG TTTGACGAGG CCGTCTTTAT CACGGTGGTA
    GACTCCTGTT CGCTCCGGAC AGATGCCCTC ATCGGGGAGT TCCGGATGGA TGTGGGTACC
    ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTGCTCTC GGACCCTGAC
    GATTTCTCTG CTGGGCCCAA AGGCTACCTG AAAGCAAGCC TTTGTGTGCT GGGGCCTGGA
    GACGAAGCTC CGCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAGCAAT
    CTGCTCAGGC CAACTGGCAT GGCCCTTCGA GGAGCGCACT TCTGTCTGAA GGTCTTCAGG
    GCTGAGGACT TACCACAGAT GGACGATGCC GTGGTGGACA GCGTGAAGCA GATCTTCGGC
    TTTGACAGTA ACAAGAAGAA CCTGGTGGAT CCCTTCGTCG AGGTCAGCTT TGCGGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGATG GCCAACCCTC AGTGGAACCA GAGCATCACG
    CTGCCTGTCA TGTTTCCCTC CATGTCTGAA AAAATGAGGA TTCGTGTCAT AGACTGGGAC
    CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA ATATGTCGAA AATCTCTGCC
    CCTGGAGGAG AAATAGCAGT GGACGACCAC CTGGGCTTCC TCCCCACCTT CGGGCCCTGC
    TATGTCAACC TCTACGGCAG CCCCAGGGAG TTCACTGGCT TCCCAGACCC CTATGCAGAG
    CTCAACACGG GCAAGGGAGA AGGCGTGGCC TACCGAGGCC GGCTTCTGGT CTCCCTGGAG
    ACCAAACTGG TGGAGCAAGG CGAGCAGAAG GTGGAGGATC TCGCTGCAGA TGACATCCTT
    CGGGTGGAGA AGTACCTCCG GAGGCGCAAG TACTCCCTCT TTGCTGCCTT CTACTCGGCC
    TGCATGCTGC AGGATGTCGA CGATGCCATC CAGTTCGAGG TCAGCATCGG AAACTACGGG
    AACAAGTTTG ACACGACCTG CCTGCCGCTG GCCTCCACCA CCCAGTACAG CCGGGCGGTC
    TTTGACGGGT GCCACTACTA CTACTTACCC TGGGGCAACG TGAAGCCTGT GGTGGTGCTG
    TCATCCTACT GGGAAGACAT CAGCCACAGA GTCGACACTC AGAACCAGCT GCTCCGGATC
    GCCGACCGGC TGGAAGCCGG CCTGGAGCAG GTCCACCTGG CCCTGAAGGC GCGGTGCTCC
    ACCGAGGATG TGGACTCCCT GGTGGCTCAG CTGATGGATG AGCTCATTGC AGACTGCAGC
    CAGCCCCTGT GCAACGTCCA AGAGACGCCC TCTGCCACCC ACCTGGACCA GTACCTGTAC
    CGGCTGCGCA CCCGTCACCT GAGCCAGATC AGTGAGGCGG CCCTGGCCTT GAAGCTCGGC
    CACAGTGAGC TCCCCGCCGC CCTTGAGCAG GCTGAGGACT GGCTCCTGCG TCTCCGTGCC
    CTGGCGGAGG AGCCCCAGAA CAGCCTGCCT GACGTGGTCA TCTGGATGCT GCAGGGAGAC
    AAGCGGGTGG CGTACCAGCG GGTGCCCGCC CACGAGGTCC TCTTCTCCCG GCGGGGCGCC
    AACTACTGTG GCAAGAATTG TGGGAAGCTG CAGACGATCT TTCTGAAGTA TCCAATGGAA
    GGGGTGCCGG GAGCCCGGAT GCCGGTGCAG ATACGGGTGA AGCTCTGGTT CGGGCTCTCT
    GTGGATGAGA AGGAGTTCAA TCAGTTTGCT GAGGGCAAGC TCTCTGTCTT CGCTGAAACC
    TACGAGAACC AGACCAAGCT GGCCCTAGTC GGGAACTGGG GCACAACGGG CCTCACCTAC
    CCCAAGTTTT CTGACGTCAC AGGCAAGATC AAGCTACCCA AGGACAGCTT CCGCCCCTCG
    GCCGGCTGGG CCTGGGCTGG AGAGTGGTTC GTGTGTCCGG AGAAGACCCT ACTCCACGAC
    ACCGATGCGG GCCACCTGAG CTTTGTGGAG GAGGTGTTTG AGAACCAGAC CCGGCTGCCC
    GGAGGCCAGT GGATCTACAT GAGTGACAAC TACACAGATG TGAACGGGGA GAAGGTGCTT
    CCCAAGGATG ACATCGAGTG CCCACTGGGC TGGAAGTGGG AAGATGAAGA ATGGTCCACA
    GACCTCAATC GGGCCGTTGA CGAGCAAGGC TGGGAGTACA GCATCACCAT CCCTCCGGAA
    CGGAAGCCAA GGCACTGGGT CCCTGCCGAG AAGATGTACT ACACACACCG TCGGCGGCGC
    TGGGTCCGCC TGCGCAGGAG GGACCTCAGC CAGATGGAGG CGCTGAAGAG GCACAGGCAG
    GCGGAGGCGG AGGGCGAGGG CTGGGAGTAC GCCTCGCTCT TCGGCTGGAA GTTCCACCTG
    GAGTACCGCA AGACCGATGC CTTCCGCCGC CGCCGCTGGC GCCGCCGCAT GGAGCCACTG
    GAGAAGACAG GGCCTGCAGC TGTGTTTGCC CTCGAGGGGG CCCTGGGCGG CGTGGTGGAC
    GACAGGAGTG AAGATTCGGT GTCCGTCTCC ACCTTGAGCT TCGGCGTGAA CAGACCCACG
    ATTTCCTGCA TCTTCGACTA CGGGAACCGC TACCATCTAC GCTGCTACAT GTACCAGGCC
    CGGGACCTGC CCGCCATGGA CAAGGACTCT TTTTCTGATC CCTACGCAAT CGTCTCCTTC
    CTGCACCAGA GCCAGAAGAC AGTGGTGGTG AAGAACACCC TGAACCCCAC CTGGGACCAG
    ACACTCATCT TCTATGAGAT CGAGATCTTT GGTGAGCCGC CCAGCATCGC CGAGCAGCCG
    CCCAGCATCG TGGTGGAGCT ATACGACCAT GACACCTACG GTGCAGATGA GTTTATGGGA
    CGCTGCATCT GTCAGCCGAC TCTGGAACGG ATGCCCCGGC TGACCTGGTT CCCACTGACT
    AGGGGCAGCC AGCCGGCGGG CGAGCTGCTG GCCTCTTTTG AGCTCATCCA GAGAGAGAAG
    CCGGCCATCT ACCACATTCC TGGTTTTGAG GTGCAGGATA CATCAGGGAT CCTGGAAGAG
    TCGGAGGACA CAGACCTGCC CTACCCGCCA CCCCAGAGAG AGGCCAACAT CTACATGGTT
    CCCCAGAACA TCAAGCCTGC TCTCCAGCGG ACGGCCATCG AGATCCTGGC ATGGGGCCTG
    CGGAACATGA AGAGTTACCA CCTGGCCAGT GTCTCCTCCC CCAGCCTCGT GGTGGAGTGC
    GGGGGCCAGA CGGTGCAGTC CTGTGTCATC AGGAACCTCC AGAAGAACCC CAACTTCGAC
    GTCTGCACCC TCTTCATGGA AGTGATGCTG CCCAGGGAGG AGCTCTACTG TCCCCCCATC
    GTGGTCAAGG TCATCGATAA CTGCCAGTTT GGCCGCCGGC CTGTGGTGGG CCAGTGTACC
    ATCTGCTCCC TGGAGAGCTT CCTGTGTGAC CCCTACTCAG AGGAGAGTCC GTCCCCACAG
    GGCGGCCCAG ATGATGTGAG CTTACTCAGT CCTGGGGAAG ATGTGCTCAT TGACATTGAT
    GACAAGGAGC CCCTCATCCC TATGCAGGAG GAGGAGTTCA TCGATTGGTG GAGCAAATTC
    TTTGCCTCGA TAGGGGAGAG GGAAAAGTGC GGGTCCTACT TGGAGAAGGA CTTTGACACT
    CTAAAGGTCT ATGACACACA ACTGGAGAAT GTGGAGGCCT TTGAGGGCCT GTCTGACTTT
    TGTAACACCT TCAAGCTCTA CCGGGGCAAG ACTCAGGAGG AGATGGAAGA CCCATCTGTC
    GTTGGGGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC GGCCATCCCC
    ATGCCCCCAA GACAGTTCCA CCAGCTAGCT GCCCAGGGAC CCCAGGAGTG CCTGGTCCGC
    ATCTACATCA TCCGGGCATT CGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGACCCT
    TACATCAAGA TCTCCATAGG GAAGAAATCC GTGAGCGACC AGGATAACTA CATCCCCTGC
    ACTCTGGAGC CTGTGTTTGG AAAGATGTTC GAACTGACCT GCACTCTGCC TCTGGAGAAG
    GACCTGAAGA TCACTCTCTA TGACTATGAC CTCCTCTCCA AGGACGAGAT GATCGGGGAG
    ACGGTCATCG ACCTGGAGAA TAGGCTGCTG TCCAAGTTTG GAGCTCGCTG CGGACTCCCA
    CAGACCTACT GTGTCTCTGG ACCGAACCAG TGGAGGGACC AGCTCCGCCC CTCCCAGCTT
    CTCCACCTGT TCTGCCAGCA GCAGAGAGTC AAGGCCCCCG TGTACCAGAC CGACCGTGTG
    GTGTTTGGGA ATAAAGAATA CACGATTGAA GAAATAGAGG CTGGGAGGGT CCTGAATCCA
    CACCTGGGCC CAGTGGAAGA GCGCCTGGCC TTGCACGTCC TCCAGCAGCA GGGCTTGGTG
    CCCGAGCATG TGGAGTCCCG GCCCCTCTAT AGCCCCCTGC AGCCGGACAT TGAGCAGGGG
    AAGCTGCAGA TGTGGATCGA CCTGTTTCCA AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC
    AACATCACTC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT GCATTATCTG GAACACCAAG
    GATGTGATCC TGGACGACCT CAGCATCACA GGAGAGAAGA TGAGCGACAT TTATGTGAAA
    GGTTGGATGG TTGGCTTTGA AGAACACAAG CAAAAGACAG ATGTGCATTA TCGGTCCCTG
    GGAGGCGAAG GGAACTTTAA CTGGAGGTTC ATTTTCCCCT TTGACTACCT GCTGGCTGAG
    CAAGTCTGCA CCGTCTCCAA GAAGGATGCT TTCTGGAAGC TGGACAAGAC CGAGAGCAAA
    ATCCCAGCAC GAGTGGTATT CCAGATCTGG GACAATGACA AGTTCTCCTT TGATGATTTC
    CTGGGCTCCT TGCAGCTGGA TCTCAACCGC ATGCCCAAGC CTGCCAAGAC AGCCGAGAAG
    TGCTCCCTGG ACCAGCTGGA TGACACTTTC CACCCAGAGT GGTTTGTGTC CCTTTTTGAA
    CAGAAAACTG TGAAGGGCTG GTGGCCCTGT GTGGCGGAAG AGGGTGAGAA GAAGAAGCTG
    GCGGGCAAGC TGGAGATGAC CTTGGAGATT GTGGCGGAGA GTGAGCATGA GGAGCGGCCC
    GCTGGCCAGG GCCGGGATGA GCCCAACATG AACCCCAAGC TCGAGGACCC AAGGCGCCCT
    GACACCTCCT TCCTCTGGTT CACCTCCCCG TACAAGACTA TGAAGTTCAT CCTGTGGCGG
    CGTTTCCGGT GTGCCATCAT CCTCTTCATC ATCCTCTTCA TCCTACTGCT GTTCTTGGGC
    ATCTTCGTTT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb49793
    Clone ID Related Accession (Same CDS sequence) XM_023618925.1
    Accession Version XM_023618925.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6270bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product dysferlin isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009158.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGAGGG TCTTTTTCCT TTACGCCGAG AACGTCCACA CCCCGGACAC CGACATCAGC 
    GATGCCTACT GCTCCGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAAAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGGGT CCCCCTGGAC
    CAGAGCTCTG AGCTTCACGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
    CTGGGAGAAG CCAAGGTCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CGCCTCTCCC TGGAGCTGTG CCCCTGTTCC CACCCTCTGT GCCGCTGGAA
    CCCTCCCCGA CTCTGCCCGA TGTGGATATG CTGGCTGATA CAGGAGGAGA GGAGGACACC
    GAGGACCAGG GGCTCACGGG AGATGAGGCA GAGCCATTCT TGGATCAGAA CGGAGCCCCA
    GGCCCCGGGG CTCCCACCAC CCTGAAGAAG CCACCTTCCC ATCCTCCCCC CTACCATCCT
    GGGGGGAAAA GGAAGAGAAG CACGCCTGCG CCCAGAAAGC TGCTTTCGGA TAAACCACAG
    GACTTCCAGA TCAGGGTCCA GGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAACATCAAG
    CCTGTGGTCA AGGTCACCGC GGCCAGGCAG ACCAAGCGGA CTCGGATTCA CAGGGGAAAC
    AGCCCGCTCT TCAACGAGAC TCTCTTCTTC AACGTGTTTG ACTCTCCCTC GGAGCTGTTT
    GACGAGGCCG TCTTTATCAC GGTGGTAGAC TCCTGTTCGC TCCGGACAGA TGCCCTCATC
    GGGGAGTTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTCAGG
    AAGTGGCTGC TGCTCTCGGA CCCTGACGAT TTCTCTGCTG GGCCCAAAGG CTACCTGAAA
    GCAAGCCTTT GTGTGCTGGG GCCTGGAGAC GAAGCTCCGC TGGAGAGAAA GGACCCCTCT
    GAAGACAAGG AGGACATTGA AAGCAATCTG CTCAGGCCAA CTGGCATGGC CCTTCGAGGA
    GCGCACTTCT GTCTGAAGGT CTTCAGGGCT GAGGACTTAC CACAGATGGA CGATGCCGTG
    GTGGACAGCG TGAAGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACCT GGTGGATCCC
    TTCGTCGAGG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGATGGCC
    AACCCTCAGT GGAACCAGAG CATCACGCTG CCTGTCATGT TTCCCTCCAT GTCTGAAAAA
    ATGAGGATTC GTGTCATAGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC
    TACCTGAATA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGCAGTGGA CGACCACCTG
    GGCTTCCTCC CCACCTTCGG GCCCTGCTAT GTCAACCTCT ACGGCAGCCC CAGGGAGTTC
    ACTGGCTTCC CAGACCCCTA TGCAGAGCTC AACACGGGCA AGGGAGAAGG CGTGGCCTAC
    CGAGGCCGGC TTCTGGTCTC CCTGGAGACC AAACTGGTGG AGCAAGGCGA GCAGAAGGTG
    GAGGATCTCG CTGCAGATGA CATCCTTCGG GTGGAGAAGT ACCTCCGGAG GCGCAAGTAC
    TCCCTCTTTG CTGCCTTCTA CTCGGCCTGC ATGCTGCAGG ATGTCGACGA TGCCATCCAG
    TTCGAGGTCA GCATCGGAAA CTACGGGAAC AAGTTTGACA CGACCTGCCT GCCGCTGGCC
    TCCACCACCC AGTACAGCCG GGCGGTCTTT GACGGGTGCC ACTACTACTA CTTACCCTGG
    GGCAACGTGA AGCCTGTGGT GGTGCTGTCA TCCTACTGGG AAGACATCAG CCACAGAGTC
    GACACTCAGA ACCAGCTGCT CCGGATCGCC GACCGGCTGG AAGCCGGCCT GGAGCAGGTC
    CACCTGGCCC TGAAGGCGCG GTGCTCCACC GAGGATGTGG ACTCCCTGGT GGCTCAGCTG
    ATGGATGAGC TCATTGCAGA CTGCAGCCAG CCCCTGTGCA ACGTCCAAGA GACGCCCTCT
    GCCACCCACC TGGACCAGTA CCTGTACCGG CTGCGCACCC GTCACCTGAG CCAGATCAGT
    GAGGCGGCCC TGGCCTTGAA GCTCGGCCAC AGTGAGCTCC CCGCCGCCCT TGAGCAGGCT
    GAGGACTGGC TCCTGCGTCT CCGTGCCCTG GCGGAGGAGC CCCAGAACAG CCTGCCTGAC
    GTGGTCATCT GGATGCTGCA GGGAGACAAG CGGGTGGCGT ACCAGCGGGT GCCCGCCCAC
    GAGGTCCTCT TCTCCCGGCG GGGCGCCAAC TACTGTGGCA AGAATTGTGG GAAGCTGCAG
    ACGATCTTTC TGAAGTATCC AATGGAAGGG GTGCCGGGAG CCCGGATGCC GGTGCAGATA
    CGGGTGAAGC TCTGGTTCGG GCTCTCTGTG GATGAGAAGG AGTTCAATCA GTTTGCTGAG
    GGCAAGCTCT CTGTCTTCGC TGAAACCTAC GAGAACCAGA CCAAGCTGGC CCTAGTCGGG
    AACTGGGGCA CAACGGGCCT CACCTACCCC AAGTTTTCTG ACGTCACAGG CAAGATCAAG
    CTACCCAAGG ACAGCTTCCG CCCCTCGGCC GGCTGGGCCT GGGCTGGAGA GTGGTTCGTG
    TGTCCGGAGA AGACCCTACT CCACGACACC GATGCGGGCC ACCTGAGCTT TGTGGAGGAG
    GTGTTTGAGA ACCAGACCCG GCTGCCCGGA GGCCAGTGGA TCTACATGAG TGACAACTAC
    ACAGATGTGA ACGGGGAGAA GGTGCTTCCC AAGGATGACA TCGAGTGCCC ACTGGGCTGG
    AAGTGGGAAG ATGAAGAATG GTCCACAGAC CTCAATCGGG CCGTTGACGA GCAAGGCTGG
    GAGTACAGCA TCACCATCCC TCCGGAACGG AAGCCAAGGC ACTGGGTCCC TGCCGAGAAG
    ATGTACTACA CACACCGTCG GCGGCGCTGG GTCCGCCTGC GCAGGAGGGA CCTCAGCCAG
    ATGGAGGCGC TGAAGAGGCA CAGGCAGGCG GAGGCGGAGG GCGAGGGCTG GGAGTACGCC
    TCGCTCTTCG GCTGGAAGTT CCACCTGGAG TACCGCAAGA CCGATGCCTT CCGCCGCCGC
    CGCTGGCGCC GCCGCATGGA GCCACTGGAG AAGACAGGGC CTGCAGCTGT GTTTGCCCTC
    GAGGGGGCCC TGGGCGGCGT GGTGGACGAC AGGAGTGAAG ATTCGGTGTC CGTCTCCACC
    TTGAGCTTCG GCGTGAACAG ACCCACGATT TCCTGCATCT TCGACTACGG GAACCGCTAC
    CATCTACGCT GCTACATGTA CCAGGCCCGG GACCTGCCCG CCATGGACAA GGACTCTTTT
    TCTGATCCCT ACGCAATCGT CTCCTTCCTG CACCAGAGCC AGAAGACAGT GGTGGTGAAG
    AACACCCTGA ACCCCACCTG GGACCAGACA CTCATCTTCT ATGAGATCGA GATCTTTGGT
    GAGCCGCCCA GCATCGCCGA GCAGCCGCCC AGCATCGTGG TGGAGCTATA CGACCATGAC
    ACCTACGGTG CAGATGAGTT TATGGGACGC TGCATCTGTC AGCCGACTCT GGAACGGATG
    CCCCGGCTGA CCTGGTTCCC ACTGACTAGG GGCAGCCAGC CGGCGGGCGA GCTGCTGGCC
    TCTTTTGAGC TCATCCAGAG AGAGAAGCCG GCCATCTACC ACATTCCTGG TTTTGAGGTG
    CAGGATACAT CAGGGATCCT GGAAGAGTCG GAGGACACAG ACCTGCCCTA CCCGCCACCC
    CAGAGAGAGG CCAACATCTA CATGGTTCCC CAGAACATCA AGCCTGCTCT CCAGCGGACG
    GCCATCGAGA TCCTGGCATG GGGCCTGCGG AACATGAAGA GTTACCACCT GGCCAGTGTC
    TCCTCCCCCA GCCTCGTGGT GGAGTGCGGG GGCCAGACGG TGCAGTCCTG TGTCATCAGG
    AACCTCCAGA AGAACCCCAA CTTCGACGTC TGCACCCTCT TCATGGAAGT GATGCTGCCC
    AGGGAGGAGC TCTACTGTCC CCCCATCGTG GTCAAGGTCA TCGATAACTG CCAGTTTGGC
    CGCCGGCCTG TGGTGGGCCA GTGTACCATC TGCTCCCTGG AGAGCTTCCT GTGTGACCCC
    TACTCAGAGG AGAGTCCGTC CCCACAGGGC GGCCCAGATG ATGTGAGCTT ACTCAGTCCT
    GGGGAAGATG TGCTCATTGA CATTGATGAC AAGGAGCCCC TCATCCCTAT GCAGCTTGCA
    GATGGTCTGT CGGGCTCGGC CCCCACTAAC ATGGCATCTT CTCCGTCCAG TCCTCATGAG
    GAGGAGTTCA TCGATTGGTG GAGCAAATTC TTTGCCTCGA TAGGGGAGAG GGAAAAGTGC
    GGGTCCTACT TGGAGAAGGA CTTTGACACT CTAAAGGTCT ATGACACACA ACTGGAGAAT
    GTGGAGGCCT TTGAGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG
    ACTCAGGAGG AGATGGAAGA CCCATCTGTC GTTGGGGAAT TTAAGGGCCT CTTCAAAATT
    TATCCCCTCC CAGAAGACCC GGCCATCCCC ATGCCCCCAA GACAGTTCCA CCAGCTAGCT
    GCCCAGGGAC CCCAGGAGTG CCTGGTCCGC ATCTACATCA TCCGGGCATT CGGCCTGCAG
    CCCAAGGACC CCAATGGGAA GTGTGACCCT TACATCAAGA TCTCCATAGG GAAGAAATCC
    GTGAGCGACC AGGATAACTA CATCCCCTGC ACTCTGGAGC CTGTGTTTGG AAAGATGTTC
    GAACTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACTCTCTA TGACTATGAC
    CTCCTCTCCA AGGACGAGAT GATCGGGGAG ACGGTCATCG ACCTGGAGAA TAGGCTGCTG
    TCCAAGTTTG GAGCTCGCTG CGGACTCCCA CAGACCTACT GTGTCTCTGG ACCGAACCAG
    TGGAGGGACC AGCTCCGCCC CTCCCAGCTT CTCCACCTGT TCTGCCAGCA GCAGAGAGTC
    AAGGCCCCCG TGTACCAGAC CGACCGTGTG GTGTTTGGGA ATAAAGAATA CACGATTGAA
    GAAATAGAGG CTGGGAGGGT CCTGAATCCA CACCTGGGCC CAGTGGAAGA GCGCCTGGCC
    TTGCACGTCC TCCAGCAGCA GGGCTTGGTG CCCGAGCATG TGGAGTCCCG GCCCCTCTAT
    AGCCCCCTGC AGCCGGACAT TGAGCAGGGG AAGCTGCAGA TGTGGATCGA CCTGTTTCCA
    AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC AACATCACTC CACGGAGAGC CAGAAGGTTT
    TTCCTGCGTT GCATTATCTG GAACACCAAG GATGTGATCC TGGACGACCT CAGCATCACA
    GGAGAGAAGA TGAGCGACAT TTATGTGAAA GGTTGGATGG TTGGCTTTGA AGAACACAAG
    CAAAAGACAG ATGTGCATTA TCGGTCCCTG GGAGGCGAAG GGAACTTTAA CTGGAGGTTC
    ATTTTCCCCT TTGACTACCT GCTGGCTGAG CAAGTCTGCA CCGTCTCCAA GAAGGATGCT
    TTCTGGAAGC TGGACAAGAC CGAGAGCAAA ATCCCAGCAC GAGTGGTATT CCAGATCTGG
    GACAATGACA AGTTCTCCTT TGATGATTTC CTGGGCTCCT TGCAGCTGGA TCTCAACCGC
    ATGCCCAAGC CTGCCAAGAC AGCCGAGAAG TGCTCCCTGG ACCAGCTGGA TGACACTTTC
    CACCCAGAGT GGTTTGTGTC CCTTTTTGAA CAGAAAACTG TGAAGGGCTG GTGGCCCTGT
    GTGGCGGAAG AGGGTGAGAA GAAGAAGCTG GCGGGCAAGC TGGAGATGAC CTTGGAGATT
    GTGGCGGAGA GTGAGCATGA GGAGCGGCCC GCTGGCCAGG GCCGGGATGA GCCCAACATG
    AACCCCAAGC TCGAGGACCC AAGGCGCCCT GACACCTCCT TCCTCTGGTT CACCTCCCCG
    TACAAGACTA TGAAGTTCAT CCTGTGGCGG CGTTTCCGGT GTGCCATCAT CCTCTTCATC
    ATCCTCTTCA TCCTACTGCT GTTCTTGGGC ATCTTCGTTT ACGCCTTCCC GAACTATGCT
    GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023474693.1
    CDS524..6793
    Translation

    Target ORF information:

    RefSeq Version XM_023618925.1
    Organism Equus caballus(horse)
    Definition Equus caballus dysferlin (DYSF), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023618925.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGAGGG TCTTTTTCCT TTACGCCGAG AACGTCCACA CCCCGGACAC CGACATCAGC 
    GATGCCTACT GCTCCGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAAAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGGGT CCCCCTGGAC
    CAGAGCTCTG AGCTTCACGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
    CTGGGAGAAG CCAAGGTCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CGCCTCTCCC TGGAGCTGTG CCCCTGTTCC CACCCTCTGT GCCGCTGGAA
    CCCTCCCCGA CTCTGCCCGA TGTGGATATG CTGGCTGATA CAGGAGGAGA GGAGGACACC
    GAGGACCAGG GGCTCACGGG AGATGAGGCA GAGCCATTCT TGGATCAGAA CGGAGCCCCA
    GGCCCCGGGG CTCCCACCAC CCTGAAGAAG CCACCTTCCC ATCCTCCCCC CTACCATCCT
    GGGGGGAAAA GGAAGAGAAG CACGCCTGCG CCCAGAAAGC TGCTTTCGGA TAAACCACAG
    GACTTCCAGA TCAGGGTCCA GGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAACATCAAG
    CCTGTGGTCA AGGTCACCGC GGCCAGGCAG ACCAAGCGGA CTCGGATTCA CAGGGGAAAC
    AGCCCGCTCT TCAACGAGAC TCTCTTCTTC AACGTGTTTG ACTCTCCCTC GGAGCTGTTT
    GACGAGGCCG TCTTTATCAC GGTGGTAGAC TCCTGTTCGC TCCGGACAGA TGCCCTCATC
    GGGGAGTTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTCAGG
    AAGTGGCTGC TGCTCTCGGA CCCTGACGAT TTCTCTGCTG GGCCCAAAGG CTACCTGAAA
    GCAAGCCTTT GTGTGCTGGG GCCTGGAGAC GAAGCTCCGC TGGAGAGAAA GGACCCCTCT
    GAAGACAAGG AGGACATTGA AAGCAATCTG CTCAGGCCAA CTGGCATGGC CCTTCGAGGA
    GCGCACTTCT GTCTGAAGGT CTTCAGGGCT GAGGACTTAC CACAGATGGA CGATGCCGTG
    GTGGACAGCG TGAAGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACCT GGTGGATCCC
    TTCGTCGAGG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGATGGCC
    AACCCTCAGT GGAACCAGAG CATCACGCTG CCTGTCATGT TTCCCTCCAT GTCTGAAAAA
    ATGAGGATTC GTGTCATAGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC
    TACCTGAATA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGCAGTGGA CGACCACCTG
    GGCTTCCTCC CCACCTTCGG GCCCTGCTAT GTCAACCTCT ACGGCAGCCC CAGGGAGTTC
    ACTGGCTTCC CAGACCCCTA TGCAGAGCTC AACACGGGCA AGGGAGAAGG CGTGGCCTAC
    CGAGGCCGGC TTCTGGTCTC CCTGGAGACC AAACTGGTGG AGCAAGGCGA GCAGAAGGTG
    GAGGATCTCG CTGCAGATGA CATCCTTCGG GTGGAGAAGT ACCTCCGGAG GCGCAAGTAC
    TCCCTCTTTG CTGCCTTCTA CTCGGCCTGC ATGCTGCAGG ATGTCGACGA TGCCATCCAG
    TTCGAGGTCA GCATCGGAAA CTACGGGAAC AAGTTTGACA CGACCTGCCT GCCGCTGGCC
    TCCACCACCC AGTACAGCCG GGCGGTCTTT GACGGGTGCC ACTACTACTA CTTACCCTGG
    GGCAACGTGA AGCCTGTGGT GGTGCTGTCA TCCTACTGGG AAGACATCAG CCACAGAGTC
    GACACTCAGA ACCAGCTGCT CCGGATCGCC GACCGGCTGG AAGCCGGCCT GGAGCAGGTC
    CACCTGGCCC TGAAGGCGCG GTGCTCCACC GAGGATGTGG ACTCCCTGGT GGCTCAGCTG
    ATGGATGAGC TCATTGCAGA CTGCAGCCAG CCCCTGTGCA ACGTCCAAGA GACGCCCTCT
    GCCACCCACC TGGACCAGTA CCTGTACCGG CTGCGCACCC GTCACCTGAG CCAGATCAGT
    GAGGCGGCCC TGGCCTTGAA GCTCGGCCAC AGTGAGCTCC CCGCCGCCCT TGAGCAGGCT
    GAGGACTGGC TCCTGCGTCT CCGTGCCCTG GCGGAGGAGC CCCAGAACAG CCTGCCTGAC
    GTGGTCATCT GGATGCTGCA GGGAGACAAG CGGGTGGCGT ACCAGCGGGT GCCCGCCCAC
    GAGGTCCTCT TCTCCCGGCG GGGCGCCAAC TACTGTGGCA AGAATTGTGG GAAGCTGCAG
    ACGATCTTTC TGAAGTATCC AATGGAAGGG GTGCCGGGAG CCCGGATGCC GGTGCAGATA
    CGGGTGAAGC TCTGGTTCGG GCTCTCTGTG GATGAGAAGG AGTTCAATCA GTTTGCTGAG
    GGCAAGCTCT CTGTCTTCGC TGAAACCTAC GAGAACCAGA CCAAGCTGGC CCTAGTCGGG
    AACTGGGGCA CAACGGGCCT CACCTACCCC AAGTTTTCTG ACGTCACAGG CAAGATCAAG
    CTACCCAAGG ACAGCTTCCG CCCCTCGGCC GGCTGGGCCT GGGCTGGAGA GTGGTTCGTG
    TGTCCGGAGA AGACCCTACT CCACGACACC GATGCGGGCC ACCTGAGCTT TGTGGAGGAG
    GTGTTTGAGA ACCAGACCCG GCTGCCCGGA GGCCAGTGGA TCTACATGAG TGACAACTAC
    ACAGATGTGA ACGGGGAGAA GGTGCTTCCC AAGGATGACA TCGAGTGCCC ACTGGGCTGG
    AAGTGGGAAG ATGAAGAATG GTCCACAGAC CTCAATCGGG CCGTTGACGA GCAAGGCTGG
    GAGTACAGCA TCACCATCCC TCCGGAACGG AAGCCAAGGC ACTGGGTCCC TGCCGAGAAG
    ATGTACTACA CACACCGTCG GCGGCGCTGG GTCCGCCTGC GCAGGAGGGA CCTCAGCCAG
    ATGGAGGCGC TGAAGAGGCA CAGGCAGGCG GAGGCGGAGG GCGAGGGCTG GGAGTACGCC
    TCGCTCTTCG GCTGGAAGTT CCACCTGGAG TACCGCAAGA CCGATGCCTT CCGCCGCCGC
    CGCTGGCGCC GCCGCATGGA GCCACTGGAG AAGACAGGGC CTGCAGCTGT GTTTGCCCTC
    GAGGGGGCCC TGGGCGGCGT GGTGGACGAC AGGAGTGAAG ATTCGGTGTC CGTCTCCACC
    TTGAGCTTCG GCGTGAACAG ACCCACGATT TCCTGCATCT TCGACTACGG GAACCGCTAC
    CATCTACGCT GCTACATGTA CCAGGCCCGG GACCTGCCCG CCATGGACAA GGACTCTTTT
    TCTGATCCCT ACGCAATCGT CTCCTTCCTG CACCAGAGCC AGAAGACAGT GGTGGTGAAG
    AACACCCTGA ACCCCACCTG GGACCAGACA CTCATCTTCT ATGAGATCGA GATCTTTGGT
    GAGCCGCCCA GCATCGCCGA GCAGCCGCCC AGCATCGTGG TGGAGCTATA CGACCATGAC
    ACCTACGGTG CAGATGAGTT TATGGGACGC TGCATCTGTC AGCCGACTCT GGAACGGATG
    CCCCGGCTGA CCTGGTTCCC ACTGACTAGG GGCAGCCAGC CGGCGGGCGA GCTGCTGGCC
    TCTTTTGAGC TCATCCAGAG AGAGAAGCCG GCCATCTACC ACATTCCTGG TTTTGAGGTG
    CAGGATACAT CAGGGATCCT GGAAGAGTCG GAGGACACAG ACCTGCCCTA CCCGCCACCC
    CAGAGAGAGG CCAACATCTA CATGGTTCCC CAGAACATCA AGCCTGCTCT CCAGCGGACG
    GCCATCGAGA TCCTGGCATG GGGCCTGCGG AACATGAAGA GTTACCACCT GGCCAGTGTC
    TCCTCCCCCA GCCTCGTGGT GGAGTGCGGG GGCCAGACGG TGCAGTCCTG TGTCATCAGG
    AACCTCCAGA AGAACCCCAA CTTCGACGTC TGCACCCTCT TCATGGAAGT GATGCTGCCC
    AGGGAGGAGC TCTACTGTCC CCCCATCGTG GTCAAGGTCA TCGATAACTG CCAGTTTGGC
    CGCCGGCCTG TGGTGGGCCA GTGTACCATC TGCTCCCTGG AGAGCTTCCT GTGTGACCCC
    TACTCAGAGG AGAGTCCGTC CCCACAGGGC GGCCCAGATG ATGTGAGCTT ACTCAGTCCT
    GGGGAAGATG TGCTCATTGA CATTGATGAC AAGGAGCCCC TCATCCCTAT GCAGCTTGCA
    GATGGTCTGT CGGGCTCGGC CCCCACTAAC ATGGCATCTT CTCCGTCCAG TCCTCATGAG
    GAGGAGTTCA TCGATTGGTG GAGCAAATTC TTTGCCTCGA TAGGGGAGAG GGAAAAGTGC
    GGGTCCTACT TGGAGAAGGA CTTTGACACT CTAAAGGTCT ATGACACACA ACTGGAGAAT
    GTGGAGGCCT TTGAGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG
    ACTCAGGAGG AGATGGAAGA CCCATCTGTC GTTGGGGAAT TTAAGGGCCT CTTCAAAATT
    TATCCCCTCC CAGAAGACCC GGCCATCCCC ATGCCCCCAA GACAGTTCCA CCAGCTAGCT
    GCCCAGGGAC CCCAGGAGTG CCTGGTCCGC ATCTACATCA TCCGGGCATT CGGCCTGCAG
    CCCAAGGACC CCAATGGGAA GTGTGACCCT TACATCAAGA TCTCCATAGG GAAGAAATCC
    GTGAGCGACC AGGATAACTA CATCCCCTGC ACTCTGGAGC CTGTGTTTGG AAAGATGTTC
    GAACTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACTCTCTA TGACTATGAC
    CTCCTCTCCA AGGACGAGAT GATCGGGGAG ACGGTCATCG ACCTGGAGAA TAGGCTGCTG
    TCCAAGTTTG GAGCTCGCTG CGGACTCCCA CAGACCTACT GTGTCTCTGG ACCGAACCAG
    TGGAGGGACC AGCTCCGCCC CTCCCAGCTT CTCCACCTGT TCTGCCAGCA GCAGAGAGTC
    AAGGCCCCCG TGTACCAGAC CGACCGTGTG GTGTTTGGGA ATAAAGAATA CACGATTGAA
    GAAATAGAGG CTGGGAGGGT CCTGAATCCA CACCTGGGCC CAGTGGAAGA GCGCCTGGCC
    TTGCACGTCC TCCAGCAGCA GGGCTTGGTG CCCGAGCATG TGGAGTCCCG GCCCCTCTAT
    AGCCCCCTGC AGCCGGACAT TGAGCAGGGG AAGCTGCAGA TGTGGATCGA CCTGTTTCCA
    AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC AACATCACTC CACGGAGAGC CAGAAGGTTT
    TTCCTGCGTT GCATTATCTG GAACACCAAG GATGTGATCC TGGACGACCT CAGCATCACA
    GGAGAGAAGA TGAGCGACAT TTATGTGAAA GGTTGGATGG TTGGCTTTGA AGAACACAAG
    CAAAAGACAG ATGTGCATTA TCGGTCCCTG GGAGGCGAAG GGAACTTTAA CTGGAGGTTC
    ATTTTCCCCT TTGACTACCT GCTGGCTGAG CAAGTCTGCA CCGTCTCCAA GAAGGATGCT
    TTCTGGAAGC TGGACAAGAC CGAGAGCAAA ATCCCAGCAC GAGTGGTATT CCAGATCTGG
    GACAATGACA AGTTCTCCTT TGATGATTTC CTGGGCTCCT TGCAGCTGGA TCTCAACCGC
    ATGCCCAAGC CTGCCAAGAC AGCCGAGAAG TGCTCCCTGG ACCAGCTGGA TGACACTTTC
    CACCCAGAGT GGTTTGTGTC CCTTTTTGAA CAGAAAACTG TGAAGGGCTG GTGGCCCTGT
    GTGGCGGAAG AGGGTGAGAA GAAGAAGCTG GCGGGCAAGC TGGAGATGAC CTTGGAGATT
    GTGGCGGAGA GTGAGCATGA GGAGCGGCCC GCTGGCCAGG GCCGGGATGA GCCCAACATG
    AACCCCAAGC TCGAGGACCC AAGGCGCCCT GACACCTCCT TCCTCTGGTT CACCTCCCCG
    TACAAGACTA TGAAGTTCAT CCTGTGGCGG CGTTTCCGGT GTGCCATCAT CCTCTTCATC
    ATCCTCTTCA TCCTACTGCT GTTCTTGGGC ATCTTCGTTT ACGCCTTCCC GAACTATGCT
    GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb49794
    Clone ID Related Accession (Same CDS sequence) XM_023618926.1
    Accession Version XM_023618926.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product dysferlin isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009158.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGAGGG TCTTTTTCCT TTACGCCGAG AACGTCCACA CCCCGGACAC CGACATCAGC 
    GATGCCTACT GCTCCGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAAAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGGGT CCCCCTGGAC
    CAGAGCTCTG AGCTTCACGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
    CTGGGAGAAG CCAAGGTCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CGCCTCTCCC TGGAGCTGTG CCCCTGTTCC CACCCTCTGT GCCGCTGGAA
    CCCTCCCCGA CTCTGCCCGA TGTGGATATG CTGGCTGATA CAGGAGGAGA GGAGGACACC
    GAGGACCAGG GGCTCACGGG AGATGAGGCA GAGCCATTCT TGGATCAGAA CGGAGCCCCA
    GGCCCCGGGG CTCCCACCAC CCTGAAGAAG CCACCTTCCC ATCCTCCCCC CTACCATCCT
    GGGGGGAAAA GGAAGAGAAG CACGCCTGCG CCCAGAAAGC TGCTTTCGGA TAAACCACAG
    GACTTCCAGA TCAGGGTCCA GGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAACATCAAG
    CCTGTGGTCA AGGTCACCGC GGCCAGGCAG ACCAAGCGGA CTCGGATTCA CAGGGGAAAC
    AGCCCGCTCT TCAACGAGAC TCTCTTCTTC AACGTGTTTG ACTCTCCCTC GGAGCTGTTT
    GACGAGGCCG TCTTTATCAC GGTGGTAGAC TCCTGTTCGC TCCGGACAGA TGCCCTCATC
    GGGGAGTTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTCAGG
    AAGTGGCTGC TGCTCTCGGA CCCTGACGAT TTCTCTGCTG GGCCCAAAGG CTACCTGAAA
    GCAAGCCTTT GTGTGCTGGG GCCTGGAGAC GAAGCTCCGC TGGAGAGAAA GGACCCCTCT
    GAAGACAAGG AGGACATTGA AAGCAATCTG CTCAGGCCAA CTGGCATGGC CCTTCGAGGA
    GCGCACTTCT GTCTGAAGGT CTTCAGGGCT GAGGACTTAC CACAGATGGA CGATGCCGTG
    GTGGACAGCG TGAAGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACCT GGTGGATCCC
    TTCGTCGAGG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGATGGCC
    AACCCTCAGT GGAACCAGAG CATCACGCTG CCTGTCATGT TTCCCTCCAT GTCTGAAAAA
    ATGAGGATTC GTGTCATAGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC
    TACCTGAATA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGCAGAGGA GCCTGCAGGT
    GTGGTCAAGC CTCCTCCAGC CACGGACCTG GACGACCACC TGGGCTTCCT CCCCACCTTC
    GGGCCCTGCT ATGTCAACCT CTACGGCAGC CCCAGGGAGT TCACTGGCTT CCCAGACCCC
    TATGCAGAGC TCAACACGGG CAAGGGAGAA GGCGTGGCCT ACCGAGGCCG GCTTCTGGTC
    TCCCTGGAGA CCAAACTGGT GGAGCAAGGC GAGCAGAAGG TGGAGGATCT CGCTGCAGAT
    GACATCCTTC GGGTGGAGAA GTACCTCCGG AGGCGCAAGT ACTCCCTCTT TGCTGCCTTC
    TACTCGGCCT GCATGCTGCA GGATGTCGAC GATGCCATCC AGTTCGAGGT CAGCATCGGA
    AACTACGGGA ACAAGTTTGA CACGACCTGC CTGCCGCTGG CCTCCACCAC CCAGTACAGC
    CGGGCGGTCT TTGACGGGTG CCACTACTAC TACTTACCCT GGGGCAACGT GAAGCCTGTG
    GTGGTGCTGT CATCCTACTG GGAAGACATC AGCCACAGAG TCGACACTCA GAACCAGCTG
    CTCCGGATCG CCGACCGGCT GGAAGCCGGC CTGGAGCAGG TCCACCTGGC CCTGAAGGCG
    CGGTGCTCCA CCGAGGATGT GGACTCCCTG GTGGCTCAGC TGATGGATGA GCTCATTGCA
    GACTGCAGCC AGCCCCTGTG CAACGTCCAA GAGACGCCCT CTGCCACCCA CCTGGACCAG
    TACCTGTACC GGCTGCGCAC CCGTCACCTG AGCCAGATCA GTGAGGCGGC CCTGGCCTTG
    AAGCTCGGCC ACAGTGAGCT CCCCGCCGCC CTTGAGCAGG CTGAGGACTG GCTCCTGCGT
    CTCCGTGCCC TGGCGGAGGA GCCCCAGAAC AGCCTGCCTG ACGTGGTCAT CTGGATGCTG
    CAGGGAGACA AGCGGGTGGC GTACCAGCGG GTGCCCGCCC ACGAGGTCCT CTTCTCCCGG
    CGGGGCGCCA ACTACTGTGG CAAGAATTGT GGGAAGCTGC AGACGATCTT TCTGAAGTAT
    CCAATGGAAG GGGTGCCGGG AGCCCGGATG CCGGTGCAGA TACGGGTGAA GCTCTGGTTC
    GGGCTCTCTG TGGATGAGAA GGAGTTCAAT CAGTTTGCTG AGGGCAAGCT CTCTGTCTTC
    GCTGAAACCT ACGAGAACCA GACCAAGCTG GCCCTAGTCG GGAACTGGGG CACAACGGGC
    CTCACCTACC CCAAGTTTTC TGACGTCACA GGCAAGATCA AGCTACCCAA GGACAGCTTC
    CGCCCCTCGG CCGGCTGGGC CTGGGCTGGA GAGTGGTTCG TGTGTCCGGA GAAGACCCTA
    CTCCACGACA CCGATGCGGG CCACCTGAGC TTTGTGGAGG AGGTGTTTGA GAACCAGACC
    CGGCTGCCCG GAGGCCAGTG GATCTACATG AGTGACAACT ACACAGATGT GAACGGGGAG
    AAGGTGCTTC CCAAGGATGA CATCGAGTGC CCACTGGGCT GGAAGTGGGA AGATGAAGAA
    TGGTCCACAG ACCTCAATCG GGCCGTTGAC GAGCAAGGCT GGGAGTACAG CATCACCATC
    CCTCCGGAAC GGAAGCCAAG GCACTGGGTC CCTGCCGAGA AGATGTACTA CACACACCGT
    CGGCGGCGCT GGGTCCGCCT GCGCAGGAGG GACCTCAGCC AGATGGAGGC GCTGAAGAGG
    CACAGGCAGG CGGAGGCGGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT CGGCTGGAAG
    TTCCACCTGG AGTACCGCAA GACCGATGCC TTCCGCCGCC GCCGCTGGCG CCGCCGCATG
    GAGCCACTGG AGAAGACAGG GCCTGCAGCT GTGTTTGCCC TCGAGGGGGC CCTGGGCGGC
    GTGGTGGACG ACAGGAGTGA AGATTCGGTG TCCGTCTCCA CCTTGAGCTT CGGCGTGAAC
    AGACCCACGA TTTCCTGCAT CTTCGACTAC GGGAACCGCT ACCATCTACG CTGCTACATG
    TACCAGGCCC GGGACCTGCC CGCCATGGAC AAGGACTCTT TTTCTGATCC CTACGCAATC
    GTCTCCTTCC TGCACCAGAG CCAGAAGACA GTGGTGGTGA AGAACACCCT GAACCCCACC
    TGGGACCAGA CACTCATCTT CTATGAGATC GAGATCTTTG GTGAGCCGCC CAGCATCGCC
    GAGCAGCCGC CCAGCATCGT GGTGGAGCTA TACGACCATG ACACCTACGG TGCAGATGAG
    TTTATGGGAC GCTGCATCTG TCAGCCGACT CTGGAACGGA TGCCCCGGCT GACCTGGTTC
    CCACTGACTA GGGGCAGCCA GCCGGCGGGC GAGCTGCTGG CCTCTTTTGA GCTCATCCAG
    AGAGAGAAGC CGGCCATCTA CCACATTCCT GGTTTTGAGG TGCAGGATAC ATCAGGGATC
    CTGGAAGAGT CGGAGGACAC AGACCTGCCC TACCCGCCAC CCCAGAGAGA GGCCAACATC
    TACATGGTTC CCCAGAACAT CAAGCCTGCT CTCCAGCGGA CGGCCATCGA GATCCTGGCA
    TGGGGCCTGC GGAACATGAA GAGTTACCAC CTGGCCAGTG TCTCCTCCCC CAGCCTCGTG
    GTGGAGTGCG GGGGCCAGAC GGTGCAGTCC TGTGTCATCA GGAACCTCCA GAAGAACCCC
    AACTTCGACG TCTGCACCCT CTTCATGGAA GTGATGCTGC CCAGGGAGGA GCTCTACTGT
    CCCCCCATCG TGGTCAAGGT CATCGATAAC TGCCAGTTTG GCCGCCGGCC TGTGGTGGGC
    CAGTGTACCA TCTGCTCCCT GGAGAGCTTC CTGTGTGACC CCTACTCAGA GGAGAGTCCG
    TCCCCACAGG GCGGCCCAGA TGATGTGAGC TTACTCAGTC CTGGGGAAGA TGTGCTCATT
    GACATTGATG ACAAGGAGCC CCTCATCCCT ATGCAGGAGG AGGAGTTCAT CGATTGGTGG
    AGCAAATTCT TTGCCTCGAT AGGGGAGAGG GAAAAGTGCG GGTCCTACTT GGAGAAGGAC
    TTTGACACTC TAAAGGTCTA TGACACACAA CTGGAGAATG TGGAGGCCTT TGAGGGCCTG
    TCTGACTTTT GTAACACCTT CAAGCTCTAC CGGGGCAAGA CTCAGGAGGA GATGGAAGAC
    CCATCTGTCG TTGGGGAATT TAAGGGCCTC TTCAAAATTT ATCCCCTCCC AGAAGACCCG
    GCCATCCCCA TGCCCCCAAG ACAGTTCCAC CAGCTAGCTG CCCAGGGACC CCAGGAGTGC
    CTGGTCCGCA TCTACATCAT CCGGGCATTC GGCCTGCAGC CCAAGGACCC CAATGGGAAG
    TGTGACCCTT ACATCAAGAT CTCCATAGGG AAGAAATCCG TGAGCGACCA GGATAACTAC
    ATCCCCTGCA CTCTGGAGCC TGTGTTTGGA AAGATGTTCG AACTGACCTG CACTCTGCCT
    CTGGAGAAGG ACCTGAAGAT CACTCTCTAT GACTATGACC TCCTCTCCAA GGACGAGATG
    ATCGGGGAGA CGGTCATCGA CCTGGAGAAT AGGCTGCTGT CCAAGTTTGG AGCTCGCTGC
    GGACTCCCAC AGACCTACTG TGTCTCTGGA CCGAACCAGT GGAGGGACCA GCTCCGCCCC
    TCCCAGCTTC TCCACCTGTT CTGCCAGCAG CAGAGAGTCA AGGCCCCCGT GTACCAGACC
    GACCGTGTGG TGTTTGGGAA TAAAGAATAC ACGATTGAAG AAATAGAGGC TGGGAGGGTC
    CTGAATCCAC ACCTGGGCCC AGTGGAAGAG CGCCTGGCCT TGCACGTCCT CCAGCAGCAG
    GGCTTGGTGC CCGAGCATGT GGAGTCCCGG CCCCTCTATA GCCCCCTGCA GCCGGACATT
    GAGCAGGGGA AGCTGCAGAT GTGGATCGAC CTGTTTCCAA AGGCCCTGGG GCGGCCTGGA
    CCTCCCTTCA ACATCACTCC ACGGAGAGCC AGAAGGTTTT TCCTGCGTTG CATTATCTGG
    AACACCAAGG ATGTGATCCT GGACGACCTC AGCATCACAG GAGAGAAGAT GAGCGACATT
    TATGTGAAAG GTTGGATGGT TGGCTTTGAA GAACACAAGC AAAAGACAGA TGTGCATTAT
    CGGTCCCTGG GAGGCGAAGG GAACTTTAAC TGGAGGTTCA TTTTCCCCTT TGACTACCTG
    CTGGCTGAGC AAGTCTGCAC CGTCTCCAAG AAGGATGCTT TCTGGAAGCT GGACAAGACC
    GAGAGCAAAA TCCCAGCACG AGTGGTATTC CAGATCTGGG ACAATGACAA GTTCTCCTTT
    GATGATTTCC TGGGCTCCTT GCAGCTGGAT CTCAACCGCA TGCCCAAGCC TGCCAAGACA
    GCCGAGAAGT GCTCCCTGGA CCAGCTGGAT GACACTTTCC ACCCAGAGTG GTTTGTGTCC
    CTTTTTGAAC AGAAAACTGT GAAGGGCTGG TGGCCCTGTG TGGCGGAAGA GGGTGAGAAG
    AAGAAGCTGG CGGGCAAGCT GGAGATGACC TTGGAGATTG TGGCGGAGAG TGAGCATGAG
    GAGCGGCCCG CTGGCCAGGG CCGGGATGAG CCCAACATGA ACCCCAAGCT CGAGGACCCA
    AGGCGCCCTG ACACCTCCTT CCTCTGGTTC ACCTCCCCGT ACAAGACTAT GAAGTTCATC
    CTGTGGCGGC GTTTCCGGTG TGCCATCATC CTCTTCATCA TCCTCTTCAT CCTACTGCTG
    TTCTTGGGCA TCTTCGTTTA CGCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC
    TTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023474694.1
    CDS523..6771
    Translation

    Target ORF information:

    RefSeq Version XM_023618926.1
    Organism Equus caballus(horse)
    Definition Equus caballus dysferlin (DYSF), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023618926.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGAGGG TCTTTTTCCT TTACGCCGAG AACGTCCACA CCCCGGACAC CGACATCAGC 
    GATGCCTACT GCTCCGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAAAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGGGT CCCCCTGGAC
    CAGAGCTCTG AGCTTCACGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
    CTGGGAGAAG CCAAGGTCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CGCCTCTCCC TGGAGCTGTG CCCCTGTTCC CACCCTCTGT GCCGCTGGAA
    CCCTCCCCGA CTCTGCCCGA TGTGGATATG CTGGCTGATA CAGGAGGAGA GGAGGACACC
    GAGGACCAGG GGCTCACGGG AGATGAGGCA GAGCCATTCT TGGATCAGAA CGGAGCCCCA
    GGCCCCGGGG CTCCCACCAC CCTGAAGAAG CCACCTTCCC ATCCTCCCCC CTACCATCCT
    GGGGGGAAAA GGAAGAGAAG CACGCCTGCG CCCAGAAAGC TGCTTTCGGA TAAACCACAG
    GACTTCCAGA TCAGGGTCCA GGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAACATCAAG
    CCTGTGGTCA AGGTCACCGC GGCCAGGCAG ACCAAGCGGA CTCGGATTCA CAGGGGAAAC
    AGCCCGCTCT TCAACGAGAC TCTCTTCTTC AACGTGTTTG ACTCTCCCTC GGAGCTGTTT
    GACGAGGCCG TCTTTATCAC GGTGGTAGAC TCCTGTTCGC TCCGGACAGA TGCCCTCATC
    GGGGAGTTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTCAGG
    AAGTGGCTGC TGCTCTCGGA CCCTGACGAT TTCTCTGCTG GGCCCAAAGG CTACCTGAAA
    GCAAGCCTTT GTGTGCTGGG GCCTGGAGAC GAAGCTCCGC TGGAGAGAAA GGACCCCTCT
    GAAGACAAGG AGGACATTGA AAGCAATCTG CTCAGGCCAA CTGGCATGGC CCTTCGAGGA
    GCGCACTTCT GTCTGAAGGT CTTCAGGGCT GAGGACTTAC CACAGATGGA CGATGCCGTG
    GTGGACAGCG TGAAGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACCT GGTGGATCCC
    TTCGTCGAGG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGATGGCC
    AACCCTCAGT GGAACCAGAG CATCACGCTG CCTGTCATGT TTCCCTCCAT GTCTGAAAAA
    ATGAGGATTC GTGTCATAGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC
    TACCTGAATA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGCAGAGGA GCCTGCAGGT
    GTGGTCAAGC CTCCTCCAGC CACGGACCTG GACGACCACC TGGGCTTCCT CCCCACCTTC
    GGGCCCTGCT ATGTCAACCT CTACGGCAGC CCCAGGGAGT TCACTGGCTT CCCAGACCCC
    TATGCAGAGC TCAACACGGG CAAGGGAGAA GGCGTGGCCT ACCGAGGCCG GCTTCTGGTC
    TCCCTGGAGA CCAAACTGGT GGAGCAAGGC GAGCAGAAGG TGGAGGATCT CGCTGCAGAT
    GACATCCTTC GGGTGGAGAA GTACCTCCGG AGGCGCAAGT ACTCCCTCTT TGCTGCCTTC
    TACTCGGCCT GCATGCTGCA GGATGTCGAC GATGCCATCC AGTTCGAGGT CAGCATCGGA
    AACTACGGGA ACAAGTTTGA CACGACCTGC CTGCCGCTGG CCTCCACCAC CCAGTACAGC
    CGGGCGGTCT TTGACGGGTG CCACTACTAC TACTTACCCT GGGGCAACGT GAAGCCTGTG
    GTGGTGCTGT CATCCTACTG GGAAGACATC AGCCACAGAG TCGACACTCA GAACCAGCTG
    CTCCGGATCG CCGACCGGCT GGAAGCCGGC CTGGAGCAGG TCCACCTGGC CCTGAAGGCG
    CGGTGCTCCA CCGAGGATGT GGACTCCCTG GTGGCTCAGC TGATGGATGA GCTCATTGCA
    GACTGCAGCC AGCCCCTGTG CAACGTCCAA GAGACGCCCT CTGCCACCCA CCTGGACCAG
    TACCTGTACC GGCTGCGCAC CCGTCACCTG AGCCAGATCA GTGAGGCGGC CCTGGCCTTG
    AAGCTCGGCC ACAGTGAGCT CCCCGCCGCC CTTGAGCAGG CTGAGGACTG GCTCCTGCGT
    CTCCGTGCCC TGGCGGAGGA GCCCCAGAAC AGCCTGCCTG ACGTGGTCAT CTGGATGCTG
    CAGGGAGACA AGCGGGTGGC GTACCAGCGG GTGCCCGCCC ACGAGGTCCT CTTCTCCCGG
    CGGGGCGCCA ACTACTGTGG CAAGAATTGT GGGAAGCTGC AGACGATCTT TCTGAAGTAT
    CCAATGGAAG GGGTGCCGGG AGCCCGGATG CCGGTGCAGA TACGGGTGAA GCTCTGGTTC
    GGGCTCTCTG TGGATGAGAA GGAGTTCAAT CAGTTTGCTG AGGGCAAGCT CTCTGTCTTC
    GCTGAAACCT ACGAGAACCA GACCAAGCTG GCCCTAGTCG GGAACTGGGG CACAACGGGC
    CTCACCTACC CCAAGTTTTC TGACGTCACA GGCAAGATCA AGCTACCCAA GGACAGCTTC
    CGCCCCTCGG CCGGCTGGGC CTGGGCTGGA GAGTGGTTCG TGTGTCCGGA GAAGACCCTA
    CTCCACGACA CCGATGCGGG CCACCTGAGC TTTGTGGAGG AGGTGTTTGA GAACCAGACC
    CGGCTGCCCG GAGGCCAGTG GATCTACATG AGTGACAACT ACACAGATGT GAACGGGGAG
    AAGGTGCTTC CCAAGGATGA CATCGAGTGC CCACTGGGCT GGAAGTGGGA AGATGAAGAA
    TGGTCCACAG ACCTCAATCG GGCCGTTGAC GAGCAAGGCT GGGAGTACAG CATCACCATC
    CCTCCGGAAC GGAAGCCAAG GCACTGGGTC CCTGCCGAGA AGATGTACTA CACACACCGT
    CGGCGGCGCT GGGTCCGCCT GCGCAGGAGG GACCTCAGCC AGATGGAGGC GCTGAAGAGG
    CACAGGCAGG CGGAGGCGGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT CGGCTGGAAG
    TTCCACCTGG AGTACCGCAA GACCGATGCC TTCCGCCGCC GCCGCTGGCG CCGCCGCATG
    GAGCCACTGG AGAAGACAGG GCCTGCAGCT GTGTTTGCCC TCGAGGGGGC CCTGGGCGGC
    GTGGTGGACG ACAGGAGTGA AGATTCGGTG TCCGTCTCCA CCTTGAGCTT CGGCGTGAAC
    AGACCCACGA TTTCCTGCAT CTTCGACTAC GGGAACCGCT ACCATCTACG CTGCTACATG
    TACCAGGCCC GGGACCTGCC CGCCATGGAC AAGGACTCTT TTTCTGATCC CTACGCAATC
    GTCTCCTTCC TGCACCAGAG CCAGAAGACA GTGGTGGTGA AGAACACCCT GAACCCCACC
    TGGGACCAGA CACTCATCTT CTATGAGATC GAGATCTTTG GTGAGCCGCC CAGCATCGCC
    GAGCAGCCGC CCAGCATCGT GGTGGAGCTA TACGACCATG ACACCTACGG TGCAGATGAG
    TTTATGGGAC GCTGCATCTG TCAGCCGACT CTGGAACGGA TGCCCCGGCT GACCTGGTTC
    CCACTGACTA GGGGCAGCCA GCCGGCGGGC GAGCTGCTGG CCTCTTTTGA GCTCATCCAG
    AGAGAGAAGC CGGCCATCTA CCACATTCCT GGTTTTGAGG TGCAGGATAC ATCAGGGATC
    CTGGAAGAGT CGGAGGACAC AGACCTGCCC TACCCGCCAC CCCAGAGAGA GGCCAACATC
    TACATGGTTC CCCAGAACAT CAAGCCTGCT CTCCAGCGGA CGGCCATCGA GATCCTGGCA
    TGGGGCCTGC GGAACATGAA GAGTTACCAC CTGGCCAGTG TCTCCTCCCC CAGCCTCGTG
    GTGGAGTGCG GGGGCCAGAC GGTGCAGTCC TGTGTCATCA GGAACCTCCA GAAGAACCCC
    AACTTCGACG TCTGCACCCT CTTCATGGAA GTGATGCTGC CCAGGGAGGA GCTCTACTGT
    CCCCCCATCG TGGTCAAGGT CATCGATAAC TGCCAGTTTG GCCGCCGGCC TGTGGTGGGC
    CAGTGTACCA TCTGCTCCCT GGAGAGCTTC CTGTGTGACC CCTACTCAGA GGAGAGTCCG
    TCCCCACAGG GCGGCCCAGA TGATGTGAGC TTACTCAGTC CTGGGGAAGA TGTGCTCATT
    GACATTGATG ACAAGGAGCC CCTCATCCCT ATGCAGGAGG AGGAGTTCAT CGATTGGTGG
    AGCAAATTCT TTGCCTCGAT AGGGGAGAGG GAAAAGTGCG GGTCCTACTT GGAGAAGGAC
    TTTGACACTC TAAAGGTCTA TGACACACAA CTGGAGAATG TGGAGGCCTT TGAGGGCCTG
    TCTGACTTTT GTAACACCTT CAAGCTCTAC CGGGGCAAGA CTCAGGAGGA GATGGAAGAC
    CCATCTGTCG TTGGGGAATT TAAGGGCCTC TTCAAAATTT ATCCCCTCCC AGAAGACCCG
    GCCATCCCCA TGCCCCCAAG ACAGTTCCAC CAGCTAGCTG CCCAGGGACC CCAGGAGTGC
    CTGGTCCGCA TCTACATCAT CCGGGCATTC GGCCTGCAGC CCAAGGACCC CAATGGGAAG
    TGTGACCCTT ACATCAAGAT CTCCATAGGG AAGAAATCCG TGAGCGACCA GGATAACTAC
    ATCCCCTGCA CTCTGGAGCC TGTGTTTGGA AAGATGTTCG AACTGACCTG CACTCTGCCT
    CTGGAGAAGG ACCTGAAGAT CACTCTCTAT GACTATGACC TCCTCTCCAA GGACGAGATG
    ATCGGGGAGA CGGTCATCGA CCTGGAGAAT AGGCTGCTGT CCAAGTTTGG AGCTCGCTGC
    GGACTCCCAC AGACCTACTG TGTCTCTGGA CCGAACCAGT GGAGGGACCA GCTCCGCCCC
    TCCCAGCTTC TCCACCTGTT CTGCCAGCAG CAGAGAGTCA AGGCCCCCGT GTACCAGACC
    GACCGTGTGG TGTTTGGGAA TAAAGAATAC ACGATTGAAG AAATAGAGGC TGGGAGGGTC
    CTGAATCCAC ACCTGGGCCC AGTGGAAGAG CGCCTGGCCT TGCACGTCCT CCAGCAGCAG
    GGCTTGGTGC CCGAGCATGT GGAGTCCCGG CCCCTCTATA GCCCCCTGCA GCCGGACATT
    GAGCAGGGGA AGCTGCAGAT GTGGATCGAC CTGTTTCCAA AGGCCCTGGG GCGGCCTGGA
    CCTCCCTTCA ACATCACTCC ACGGAGAGCC AGAAGGTTTT TCCTGCGTTG CATTATCTGG
    AACACCAAGG ATGTGATCCT GGACGACCTC AGCATCACAG GAGAGAAGAT GAGCGACATT
    TATGTGAAAG GTTGGATGGT TGGCTTTGAA GAACACAAGC AAAAGACAGA TGTGCATTAT
    CGGTCCCTGG GAGGCGAAGG GAACTTTAAC TGGAGGTTCA TTTTCCCCTT TGACTACCTG
    CTGGCTGAGC AAGTCTGCAC CGTCTCCAAG AAGGATGCTT TCTGGAAGCT GGACAAGACC
    GAGAGCAAAA TCCCAGCACG AGTGGTATTC CAGATCTGGG ACAATGACAA GTTCTCCTTT
    GATGATTTCC TGGGCTCCTT GCAGCTGGAT CTCAACCGCA TGCCCAAGCC TGCCAAGACA
    GCCGAGAAGT GCTCCCTGGA CCAGCTGGAT GACACTTTCC ACCCAGAGTG GTTTGTGTCC
    CTTTTTGAAC AGAAAACTGT GAAGGGCTGG TGGCCCTGTG TGGCGGAAGA GGGTGAGAAG
    AAGAAGCTGG CGGGCAAGCT GGAGATGACC TTGGAGATTG TGGCGGAGAG TGAGCATGAG
    GAGCGGCCCG CTGGCCAGGG CCGGGATGAG CCCAACATGA ACCCCAAGCT CGAGGACCCA
    AGGCGCCCTG ACACCTCCTT CCTCTGGTTC ACCTCCCCGT ACAAGACTAT GAAGTTCATC
    CTGTGGCGGC GTTTCCGGTG TGCCATCATC CTCTTCATCA TCCTCTTCAT CCTACTGCTG
    TTCTTGGGCA TCTTCGTTTA CGCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC
    TTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb49795
    Clone ID Related Accession (Same CDS sequence) XM_023618927.1
    Accession Version XM_023618927.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product dysferlin isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009158.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTGAGGG TCTTTTTCCT TTACGCCGAG AACGTCCACA CCCCGGACAC CGACATCAGC 
    GATGCCTACT GCTCCGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAAAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGGGT CCCCCTGGAC
    CAGAGCTCTG AGCTTCACGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
    CTGGGAGAAG CCAAGGTCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CGCCTCTCCC TGGAGCTGTG CCCCTGTTCC CACCCTCTGT GCCGCTGGAA
    CCCTCCCCGA CTCTGCCCGA TGTGGATATG CTGGCTGATA CAGGAGGAGA GGAGGACACC
    GAGGACCAGG GGCTCACGGG AGATGAGGCA GAGCCATTCT TGGATCAGAA CGGAGCCCCA
    GGCCCCGGGG CTCCCACCAC CCTGAAGAAG CCACCTTCCC ATCCTCCCCC CTACCATCCT
    GGGGGGAAAA GGAAGAGAAG CACGCCTGCG CCCAGAAAGC TGCTTTCGGA TAAACCACAG
    GACTTCCAGA TCAGGGTCCA GGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAACATCAAG
    CCTGTGGTCA AGGTCACCGC GGCCAGGCAG ACCAAGCGGA CTCGGATTCA CAGGGGAAAC
    AGCCCGCTCT TCAACGAGAC TCTCTTCTTC AACGTGTTTG ACTCTCCCTC GGAGCTGTTT
    GACGAGGCCG TCTTTATCAC GGTGGTAGAC TCCTGTTCGC TCCGGACAGA TGCCCTCATC
    GGGGAGTTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTCAGG
    AAGTGGCTGC TGCTCTCGGA CCCTGACGAT TTCTCTGCTG GGCCCAAAGG CTACCTGAAA
    GCAAGCCTTT GTGTGCTGGG GCCTGGAGAC GAAGCTCCGC TGGAGAGAAA GGACCCCTCT
    GAAGACAAGG AGGACATTGA AAGCAATCTG CTCAGGCCAA CTGGCATGGC CCTTCGAGGA
    GCGCACTTCT GTCTGAAGGT CTTCAGGGCT GAGGACTTAC CACAGATGGA CGATGCCGTG
    GTGGACAGCG TGAAGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACCT GGTGGATCCC
    TTCGTCGAGG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGATGGCC
    AACCCTCAGT GGAACCAGAG CATCACGCTG CCTGTCATGT TTCCCTCCAT GTCTGAAAAA
    ATGAGGATTC GTGTCATAGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC
    TACCTGAATA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGCAGTGGA CGACCACCTG
    GGCTTCCTCC CCACCTTCGG GCCCTGCTAT GTCAACCTCT ACGGCAGCCC CAGGGAGTTC
    ACTGGCTTCC CAGACCCCTA TGCAGAGCTC AACACGGGCA AGGGAGAAGG CGTGGCCTAC
    CGAGGCCGGC TTCTGGTCTC CCTGGAGACC AAACTGGTGG AGCAAGGCGA GCAGAAGGTG
    GAGGATCTCG CTGCAGATGA CATCCTTCGG GTGGAGAAGT ACCTCCGGAG GCGCAAGTAC
    TCCCTCTTTG CTGCCTTCTA CTCGGCCTGC ATGCTGCAGG ATGTCGACGA TGCCATCCAG
    TTCGAGGTCA GCATCGGAAA CTACGGGAAC AAGTTTGACA CGACCTGCCT GCCGCTGGCC
    TCCACCACCC AGTACAGCCG GGCGGTCTTT GACGGGTGCC ACTACTACTA CTTACCCTGG
    GGCAACGTGA AGCCTGTGGT GGTGCTGTCA TCCTACTGGG AAGACATCAG CCACAGAGTC
    GACACTCAGA ACCAGCTGCT CCGGATCGCC GACCGGCTGG AAGCCGGCCT GGAGCAGGTC
    CACCTGGCCC TGAAGGCGCG GTGCTCCACC GAGGATGTGG ACTCCCTGGT GGCTCAGCTG
    ATGGATGAGC TCATTGCAGA CTGCAGCCAG CCCCTGTGCA ACGTCCAAGA GACGCCCTCT
    GCCACCCACC TGGACCAGTA CCTGTACCGG CTGCGCACCC GTCACCTGAG CCAGATCAGT
    GAGGCGGCCC TGGCCTTGAA GCTCGGCCAC AGTGAGCTCC CCGCCGCCCT TGAGCAGGCT
    GAGGACTGGC TCCTGCGTCT CCGTGCCCTG GCGGAGGAGC CCCAGAACAG CCTGCCTGAC
    GTGGTCATCT GGATGCTGCA GGGAGACAAG CGGGTGGCGT ACCAGCGGGT GCCCGCCCAC
    GAGGTCCTCT TCTCCCGGCG GGGCGCCAAC TACTGTGGCA AGAATTGTGG GAAGCTGCAG
    ACGATCTTTC TGAAGTATCC AATGGAAGGG GTGCCGGGAG CCCGGATGCC GGTGCAGATA
    CGGGTGAAGC TCTGGTTCGG GCTCTCTGTG GATGAGAAGG AGTTCAATCA GTTTGCTGAG
    GGCAAGCTCT CTGTCTTCGC TGAAACCTAC GAGAACCAGA CCAAGCTGGC CCTAGTCGGG
    AACTGGGGCA CAACGGGCCT CACCTACCCC AAGTTTTCTG ACGTCACAGG CAAGATCAAG
    CTACCCAAGG ACAGCTTCCG CCCCTCGGCC GGCTGGGCCT GGGCTGGAGA GTGGTTCGTG
    TGTCCGGAGA AGACCCTACT CCACGACACC GATGCGGGCC ACCTGAGCTT TGTGGAGGAG
    GTGTTTGAGA ACCAGACCCG GCTGCCCGGA GGCCAGTGGA TCTACATGAG TGACAACTAC
    ACAGATGTGA ACGGGGAGAA GGTGCTTCCC AAGGATGACA TCGAGTGCCC ACTGGGCTGG
    AAGTGGGAAG ATGAAGAATG GTCCACAGAC CTCAATCGGG CCGTTGACGA GCAAGGCTGG
    GAGTACAGCA TCACCATCCC TCCGGAACGG AAGCCAAGGC ACTGGGTCCC TGCCGAGAAG
    ATGTACTACA CACACCGTCG GCGGCGCTGG GTCCGCCTGC GCAGGAGGGA CCTCAGCCAG
    ATGGAGGCGC TGAAGAGGCA CAGGCAGGCG GAGGCGGAGG GCGAGGGCTG GGAGTACGCC
    TCGCTCTTCG GCTGGAAGTT CCACCTGGAG TACCGCAAGA CCGATGCCTT CCGCCGCCGC
    CGCTGGCGCC GCCGCATGGA GCCACTGGAG AAGACAGGGC CTGCAGCTGT GTTTGCCCTC
    GAGGGGGCCC TGGGCGGCGT GGTGGACGAC AGGAGTGAAG ATTCGGTGTC CGTCTCCACC
    TTGAGCTTCG GCGTGAACAG ACCCACGATT TCCTGCATCT TCGACTACGG GAACCGCTAC
    CATCTACGCT GCTACATGTA CCAGGCCCGG GACCTGCCCG CCATGGACAA GGACTCTTTT
    TCTGATCCCT ACGCAATCGT CTCCTTCCTG CACCAGAGCC AGAAGACAGT GGTGGTGAAG
    AACACCCTGA ACCCCACCTG GGACCAGACA CTCATCTTCT ATGAGATCGA GATCTTTGGT
    GAGCCGCCCA GCATCGCCGA GCAGCCGCCC AGCATCGTGG TGGAGCTATA CGACCATGAC
    ACCTACGGTG CAGATGAGTT TATGGGACGC TGCATCTGTC AGCCGACTCT GGAACGGATG
    CCCCGGCTGA CCTGGTTCCC ACTGACTAGG GGCAGCCAGC CGGCGGGCGA GCTGCTGGCC
    TCTTTTGAGC TCATCCAGAG AGAGAAGCCG GCCATCTACC ACATTCCTGG TTTTGAGGTG
    CAGGATACAT CAGGGATCCT GGAAGAGTCG GAGGACACAG ACCTGCCCTA CCCGCCACCC
    CAGAGAGAGG CCAACATCTA CATGGTTCCC CAGAACATCA AGCCTGCTCT CCAGCGGACG
    GCCATCGAGA TCCTGGCATG GGGCCTGCGG AACATGAAGA GTTACCACCT GGCCAGTGTC
    TCCTCCCCCA GCCTCGTGGT GGAGTGCGGG GGCCAGACGG TGCAGTCCTG TGTCATCAGG
    AACCTCCAGA AGAACCCCAA CTTCGACGTC TGCACCCTCT TCATGGAAGT GATGCTGCCC
    AGGGAGGAGC TCTACTGTCC CCCCATCGTG GTCAAGGTCA TCGATAACTG CCAGTTTGGC
    CGCCGGCCTG TGGTGGGCCA GTGTACCATC TGCTCCCTGG AGAGCTTCCT GTGTGACCCC
    TACTCAGAGG AGAGTCCGTC CCCACAGGGC GGCCCAGATG ATGTGAGCTT ACTCAGTCCT
    GGGGAAGATG TGCTCATTGA CATTGATGAC AAGGAGCCCC TCATCCCTAT GCAGGAGGAG
    GAGTTCATCG ATTGGTGGAG CAAATTCTTT GCCTCGATAG GGGAGAGGGA AAAGTGCGGG
    TCCTACTTGG AGAAGGACTT TGACACTCTA AAGGTCTATG ACACACAACT GGAGAATGTG
    GAGGCCTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTCTACCG GGGCAAGACT
    CAGGAGGAGA TGGAAGACCC ATCTGTCGTT GGGGAATTTA AGGGCCTCTT CAAAATTTAT
    CCCCTCCCAG AAGACCCGGC CATCCCCATG CCCCCAAGAC AGTTCCACCA GCTAGCTGCC
    CAGGGACCCC AGGAGTGCCT GGTCCGCATC TACATCATCC GGGCATTCGG CCTGCAGCCC
    AAGGACCCCA ATGGGAAGTG TGACCCTTAC ATCAAGATCT CCATAGGGAA GAAATCCGTG
    AGCGACCAGG ATAACTACAT CCCCTGCACT CTGGAGCCTG TGTTTGGAAA GATGTTCGAA
    CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGATCA CTCTCTATGA CTATGACCTC
    CTCTCCAAGG ACGAGATGAT CGGGGAGACG GTCATCGACC TGGAGAATAG GCTGCTGTCC
    AAGTTTGGAG CTCGCTGCGG ACTCCCACAG ACCTACTGTG TCTCTGGACC GAACCAGTGG
    AGGGACCAGC TCCGCCCCTC CCAGCTTCTC CACCTGTTCT GCCAGCAGCA GAGAGTCAAG
    GCCCCCGTGT ACCAGACCGA CCGTGTGGTG TTTGGGAATA AAGAATACAC GATTGAAGAA
    ATAGAGGCTG GGAGGGTCCT GAATCCACAC CTGGGCCCAG TGGAAGAGCG CCTGGCCTTG
    CACGTCCTCC AGCAGCAGGG CTTGGTGCCC GAGCATGTGG AGTCCCGGCC CCTCTATAGC
    CCCCTGCAGC CGGACATTGA GCAGGGGAAG CTGCAGATGT GGATCGACCT GTTTCCAAAG
    GCCCTGGGGC GGCCTGGACC TCCCTTCAAC ATCACTCCAC GGAGAGCCAG AAGGTTTTTC
    CTGCGTTGCA TTATCTGGAA CACCAAGGAT GTGATCCTGG ACGACCTCAG CATCACAGGA
    GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGGTTG GCTTTGAAGA ACACAAGCAA
    AAGACAGATG TGCATTATCG GTCCCTGGGA GGCGAAGGGA ACTTTAACTG GAGGTTCATT
    TTCCCCTTTG ACTACCTGCT GGCTGAGCAA GTCTGCACCG TCTCCAAGAA GGATGCTTTC
    TGGAAGCTGG ACAAGACCGA GAGCAAAATC CCAGCACGAG TGGTATTCCA GATCTGGGAC
    AATGACAAGT TCTCCTTTGA TGATTTCCTG GGCTCCTTGC AGCTGGATCT CAACCGCATG
    CCCAAGCCTG CCAAGACAGC CGAGAAGTGC TCCCTGGACC AGCTGGATGA CACTTTCCAC
    CCAGAGTGGT TTGTGTCCCT TTTTGAACAG AAAACTGTGA AGGGCTGGTG GCCCTGTGTG
    GCGGAAGAGG GTGAGAAGAA GAAGCTGGCG GGCAAGCTGG AGATGACCTT GGAGATTGTG
    GCGGAGAGTG AGCATGAGGA GCGGCCCGCT GGCCAGGGCC GGGATGAGCC CAACATGAAC
    CCCAAGCTCG AGGACCCAAG GCGCCCTGAC ACCTCCTTCC TCTGGTTCAC CTCCCCGTAC
    AAGACTATGA AGTTCATCCT GTGGCGGCGT TTCCGGTGTG CCATCATCCT CTTCATCATC
    CTCTTCATCC TACTGCTGTT CTTGGGCATC TTCGTTTACG CCTTCCCGAA CTATGCTGCC
    ATGAAGCTGG TGAAGCCCTT CAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023474695.1
    CDS523..6729
    Translation

    Target ORF information:

    RefSeq Version XM_023618927.1
    Organism Equus caballus(horse)
    Definition Equus caballus dysferlin (DYSF), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023618927.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTGAGGG TCTTTTTCCT TTACGCCGAG AACGTCCACA CCCCGGACAC CGACATCAGC 
    GATGCCTACT GCTCCGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAAAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGGGT CCCCCTGGAC
    CAGAGCTCTG AGCTTCACGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
    CTGGGAGAAG CCAAGGTCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CGCCTCTCCC TGGAGCTGTG CCCCTGTTCC CACCCTCTGT GCCGCTGGAA
    CCCTCCCCGA CTCTGCCCGA TGTGGATATG CTGGCTGATA CAGGAGGAGA GGAGGACACC
    GAGGACCAGG GGCTCACGGG AGATGAGGCA GAGCCATTCT TGGATCAGAA CGGAGCCCCA
    GGCCCCGGGG CTCCCACCAC CCTGAAGAAG CCACCTTCCC ATCCTCCCCC CTACCATCCT
    GGGGGGAAAA GGAAGAGAAG CACGCCTGCG CCCAGAAAGC TGCTTTCGGA TAAACCACAG
    GACTTCCAGA TCAGGGTCCA GGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAACATCAAG
    CCTGTGGTCA AGGTCACCGC GGCCAGGCAG ACCAAGCGGA CTCGGATTCA CAGGGGAAAC
    AGCCCGCTCT TCAACGAGAC TCTCTTCTTC AACGTGTTTG ACTCTCCCTC GGAGCTGTTT
    GACGAGGCCG TCTTTATCAC GGTGGTAGAC TCCTGTTCGC TCCGGACAGA TGCCCTCATC
    GGGGAGTTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTCAGG
    AAGTGGCTGC TGCTCTCGGA CCCTGACGAT TTCTCTGCTG GGCCCAAAGG CTACCTGAAA
    GCAAGCCTTT GTGTGCTGGG GCCTGGAGAC GAAGCTCCGC TGGAGAGAAA GGACCCCTCT
    GAAGACAAGG AGGACATTGA AAGCAATCTG CTCAGGCCAA CTGGCATGGC CCTTCGAGGA
    GCGCACTTCT GTCTGAAGGT CTTCAGGGCT GAGGACTTAC CACAGATGGA CGATGCCGTG
    GTGGACAGCG TGAAGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACCT GGTGGATCCC
    TTCGTCGAGG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGATGGCC
    AACCCTCAGT GGAACCAGAG CATCACGCTG CCTGTCATGT TTCCCTCCAT GTCTGAAAAA
    ATGAGGATTC GTGTCATAGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC
    TACCTGAATA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGCAGTGGA CGACCACCTG
    GGCTTCCTCC CCACCTTCGG GCCCTGCTAT GTCAACCTCT ACGGCAGCCC CAGGGAGTTC
    ACTGGCTTCC CAGACCCCTA TGCAGAGCTC AACACGGGCA AGGGAGAAGG CGTGGCCTAC
    CGAGGCCGGC TTCTGGTCTC CCTGGAGACC AAACTGGTGG AGCAAGGCGA GCAGAAGGTG
    GAGGATCTCG CTGCAGATGA CATCCTTCGG GTGGAGAAGT ACCTCCGGAG GCGCAAGTAC
    TCCCTCTTTG CTGCCTTCTA CTCGGCCTGC ATGCTGCAGG ATGTCGACGA TGCCATCCAG
    TTCGAGGTCA GCATCGGAAA CTACGGGAAC AAGTTTGACA CGACCTGCCT GCCGCTGGCC
    TCCACCACCC AGTACAGCCG GGCGGTCTTT GACGGGTGCC ACTACTACTA CTTACCCTGG
    GGCAACGTGA AGCCTGTGGT GGTGCTGTCA TCCTACTGGG AAGACATCAG CCACAGAGTC
    GACACTCAGA ACCAGCTGCT CCGGATCGCC GACCGGCTGG AAGCCGGCCT GGAGCAGGTC
    CACCTGGCCC TGAAGGCGCG GTGCTCCACC GAGGATGTGG ACTCCCTGGT GGCTCAGCTG
    ATGGATGAGC TCATTGCAGA CTGCAGCCAG CCCCTGTGCA ACGTCCAAGA GACGCCCTCT
    GCCACCCACC TGGACCAGTA CCTGTACCGG CTGCGCACCC GTCACCTGAG CCAGATCAGT
    GAGGCGGCCC TGGCCTTGAA GCTCGGCCAC AGTGAGCTCC CCGCCGCCCT TGAGCAGGCT
    GAGGACTGGC TCCTGCGTCT CCGTGCCCTG GCGGAGGAGC CCCAGAACAG CCTGCCTGAC
    GTGGTCATCT GGATGCTGCA GGGAGACAAG CGGGTGGCGT ACCAGCGGGT GCCCGCCCAC
    GAGGTCCTCT TCTCCCGGCG GGGCGCCAAC TACTGTGGCA AGAATTGTGG GAAGCTGCAG
    ACGATCTTTC TGAAGTATCC AATGGAAGGG GTGCCGGGAG CCCGGATGCC GGTGCAGATA
    CGGGTGAAGC TCTGGTTCGG GCTCTCTGTG GATGAGAAGG AGTTCAATCA GTTTGCTGAG
    GGCAAGCTCT CTGTCTTCGC TGAAACCTAC GAGAACCAGA CCAAGCTGGC CCTAGTCGGG
    AACTGGGGCA CAACGGGCCT CACCTACCCC AAGTTTTCTG ACGTCACAGG CAAGATCAAG
    CTACCCAAGG ACAGCTTCCG CCCCTCGGCC GGCTGGGCCT GGGCTGGAGA GTGGTTCGTG
    TGTCCGGAGA AGACCCTACT CCACGACACC GATGCGGGCC ACCTGAGCTT TGTGGAGGAG
    GTGTTTGAGA ACCAGACCCG GCTGCCCGGA GGCCAGTGGA TCTACATGAG TGACAACTAC
    ACAGATGTGA ACGGGGAGAA GGTGCTTCCC AAGGATGACA TCGAGTGCCC ACTGGGCTGG
    AAGTGGGAAG ATGAAGAATG GTCCACAGAC CTCAATCGGG CCGTTGACGA GCAAGGCTGG
    GAGTACAGCA TCACCATCCC TCCGGAACGG AAGCCAAGGC ACTGGGTCCC TGCCGAGAAG
    ATGTACTACA CACACCGTCG GCGGCGCTGG GTCCGCCTGC GCAGGAGGGA CCTCAGCCAG
    ATGGAGGCGC TGAAGAGGCA CAGGCAGGCG GAGGCGGAGG GCGAGGGCTG GGAGTACGCC
    TCGCTCTTCG GCTGGAAGTT CCACCTGGAG TACCGCAAGA CCGATGCCTT CCGCCGCCGC
    CGCTGGCGCC GCCGCATGGA GCCACTGGAG AAGACAGGGC CTGCAGCTGT GTTTGCCCTC
    GAGGGGGCCC TGGGCGGCGT GGTGGACGAC AGGAGTGAAG ATTCGGTGTC CGTCTCCACC
    TTGAGCTTCG GCGTGAACAG ACCCACGATT TCCTGCATCT TCGACTACGG GAACCGCTAC
    CATCTACGCT GCTACATGTA CCAGGCCCGG GACCTGCCCG CCATGGACAA GGACTCTTTT
    TCTGATCCCT ACGCAATCGT CTCCTTCCTG CACCAGAGCC AGAAGACAGT GGTGGTGAAG
    AACACCCTGA ACCCCACCTG GGACCAGACA CTCATCTTCT ATGAGATCGA GATCTTTGGT
    GAGCCGCCCA GCATCGCCGA GCAGCCGCCC AGCATCGTGG TGGAGCTATA CGACCATGAC
    ACCTACGGTG CAGATGAGTT TATGGGACGC TGCATCTGTC AGCCGACTCT GGAACGGATG
    CCCCGGCTGA CCTGGTTCCC ACTGACTAGG GGCAGCCAGC CGGCGGGCGA GCTGCTGGCC
    TCTTTTGAGC TCATCCAGAG AGAGAAGCCG GCCATCTACC ACATTCCTGG TTTTGAGGTG
    CAGGATACAT CAGGGATCCT GGAAGAGTCG GAGGACACAG ACCTGCCCTA CCCGCCACCC
    CAGAGAGAGG CCAACATCTA CATGGTTCCC CAGAACATCA AGCCTGCTCT CCAGCGGACG
    GCCATCGAGA TCCTGGCATG GGGCCTGCGG AACATGAAGA GTTACCACCT GGCCAGTGTC
    TCCTCCCCCA GCCTCGTGGT GGAGTGCGGG GGCCAGACGG TGCAGTCCTG TGTCATCAGG
    AACCTCCAGA AGAACCCCAA CTTCGACGTC TGCACCCTCT TCATGGAAGT GATGCTGCCC
    AGGGAGGAGC TCTACTGTCC CCCCATCGTG GTCAAGGTCA TCGATAACTG CCAGTTTGGC
    CGCCGGCCTG TGGTGGGCCA GTGTACCATC TGCTCCCTGG AGAGCTTCCT GTGTGACCCC
    TACTCAGAGG AGAGTCCGTC CCCACAGGGC GGCCCAGATG ATGTGAGCTT ACTCAGTCCT
    GGGGAAGATG TGCTCATTGA CATTGATGAC AAGGAGCCCC TCATCCCTAT GCAGGAGGAG
    GAGTTCATCG ATTGGTGGAG CAAATTCTTT GCCTCGATAG GGGAGAGGGA AAAGTGCGGG
    TCCTACTTGG AGAAGGACTT TGACACTCTA AAGGTCTATG ACACACAACT GGAGAATGTG
    GAGGCCTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTCTACCG GGGCAAGACT
    CAGGAGGAGA TGGAAGACCC ATCTGTCGTT GGGGAATTTA AGGGCCTCTT CAAAATTTAT
    CCCCTCCCAG AAGACCCGGC CATCCCCATG CCCCCAAGAC AGTTCCACCA GCTAGCTGCC
    CAGGGACCCC AGGAGTGCCT GGTCCGCATC TACATCATCC GGGCATTCGG CCTGCAGCCC
    AAGGACCCCA ATGGGAAGTG TGACCCTTAC ATCAAGATCT CCATAGGGAA GAAATCCGTG
    AGCGACCAGG ATAACTACAT CCCCTGCACT CTGGAGCCTG TGTTTGGAAA GATGTTCGAA
    CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGATCA CTCTCTATGA CTATGACCTC
    CTCTCCAAGG ACGAGATGAT CGGGGAGACG GTCATCGACC TGGAGAATAG GCTGCTGTCC
    AAGTTTGGAG CTCGCTGCGG ACTCCCACAG ACCTACTGTG TCTCTGGACC GAACCAGTGG
    AGGGACCAGC TCCGCCCCTC CCAGCTTCTC CACCTGTTCT GCCAGCAGCA GAGAGTCAAG
    GCCCCCGTGT ACCAGACCGA CCGTGTGGTG TTTGGGAATA AAGAATACAC GATTGAAGAA
    ATAGAGGCTG GGAGGGTCCT GAATCCACAC CTGGGCCCAG TGGAAGAGCG CCTGGCCTTG
    CACGTCCTCC AGCAGCAGGG CTTGGTGCCC GAGCATGTGG AGTCCCGGCC CCTCTATAGC
    CCCCTGCAGC CGGACATTGA GCAGGGGAAG CTGCAGATGT GGATCGACCT GTTTCCAAAG
    GCCCTGGGGC GGCCTGGACC TCCCTTCAAC ATCACTCCAC GGAGAGCCAG AAGGTTTTTC
    CTGCGTTGCA TTATCTGGAA CACCAAGGAT GTGATCCTGG ACGACCTCAG CATCACAGGA
    GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGGTTG GCTTTGAAGA ACACAAGCAA
    AAGACAGATG TGCATTATCG GTCCCTGGGA GGCGAAGGGA ACTTTAACTG GAGGTTCATT
    TTCCCCTTTG ACTACCTGCT GGCTGAGCAA GTCTGCACCG TCTCCAAGAA GGATGCTTTC
    TGGAAGCTGG ACAAGACCGA GAGCAAAATC CCAGCACGAG TGGTATTCCA GATCTGGGAC
    AATGACAAGT TCTCCTTTGA TGATTTCCTG GGCTCCTTGC AGCTGGATCT CAACCGCATG
    CCCAAGCCTG CCAAGACAGC CGAGAAGTGC TCCCTGGACC AGCTGGATGA CACTTTCCAC
    CCAGAGTGGT TTGTGTCCCT TTTTGAACAG AAAACTGTGA AGGGCTGGTG GCCCTGTGTG
    GCGGAAGAGG GTGAGAAGAA GAAGCTGGCG GGCAAGCTGG AGATGACCTT GGAGATTGTG
    GCGGAGAGTG AGCATGAGGA GCGGCCCGCT GGCCAGGGCC GGGATGAGCC CAACATGAAC
    CCCAAGCTCG AGGACCCAAG GCGCCCTGAC ACCTCCTTCC TCTGGTTCAC CTCCCCGTAC
    AAGACTATGA AGTTCATCCT GTGGCGGCGT TTCCGGTGTG CCATCATCCT CTTCATCATC
    CTCTTCATCC TACTGCTGTT CTTGGGCATC TTCGTTTACG CCTTCCCGAA CTATGCTGCC
    ATGAAGCTGG TGAAGCCCTT CAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.