MBD5 cDNA ORF clone, Equus caballus(horse)

Gene
Clones
gRNAs

Summary

Gene Symbol	MBD5
Entrez Gene ID	100049894
Full Name	methyl-CpG binding domain protein 5
General protein information	Preferred Names methyl-CpG binding domain protein 5 Names methyl-CpG-binding domain protein 5
Gene Type	protein-coding
Organism	Equus caballus(horse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014732457.1	XP_014587943.1	methyl-CpG-binding domain protein 5 isoform X2
XM_014732456.1	XP_014587942.1	methyl-CpG-binding domain protein 5 isoform X1
XM_023622554.1	XP_023478322.1	methyl-CpG-binding domain protein 5 isoform X1
XM_005601478.2	XP_005601535.1	methyl-CpG-binding domain protein 5 isoform X1
XM_005601478.3	XP_005601535.1	methyl-CpG-binding domain protein 5 isoform X1
XM_014732456.2	XP_014587942.1	methyl-CpG-binding domain protein 5 isoform X1
XM_023622555.1	XP_023478323.1	methyl-CpG-binding domain protein 5 isoform X2

Pathways from BioSystems
Gene Ontology

The following MBD5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MBD5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHb36289	XM_014732457.1 Latest version!	Equus caballus methyl-CpG binding domain protein 5 (MBD5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OHb52493	XM_023622555.1 Latest version!	Equus caballus methyl-CpG binding domain protein 5 (MBD5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHb08212	XM_005601478.3 Latest version!	Equus caballus methyl-CpG binding domain protein 5 (MBD5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OHb08212	XM_014732456.2 Latest version!	Equus caballus methyl-CpG binding domain protein 5 (MBD5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OHb08212	XM_023622554.1 Latest version!	Equus caballus methyl-CpG binding domain protein 5 (MBD5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
OHb08212	XM_005601478.2	Equus caballus methyl-CpG binding domain protein 5 (MBD5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OHb08212	XM_014732456.1	Equus caballus methyl-CpG binding domain protein 5 (MBD5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MBD5 ( XM_014732457.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_014587943.1 Equus caballus methyl-CpG binding domain protein 5 (MBD5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHb36289
Clone ID Related Accession (Same CDS sequence)	XM_014732457.1
Accession Version	XM_014732457.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	methyl-CpG-binding domain protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867384.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTGTACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGACGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATCAT TGCAGTGGCA ACACTTCATA AAAGCATGGA AGCTCCACAT 
CCTTCACTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA 
CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
ATGCCCAGCC CAGCGTCGTC AGGTTCCCAG ATATATGGAG ATGGCTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAGTAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACCAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAAGTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACTTCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACTAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA 
TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG 
GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAATAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA CAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGCGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA 
CAGCCACCTA CCACAGTGTT GAGTTTGCTC AGACAGTCGC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCCGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTGCC 
CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAATA TGAACTCCAG TGTTCTTCAG AATTCACTGA CACAGAACAT ACCTTTAAGA 
GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC 
CCGAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG 
CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA 
ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA 
AGTTCTGAAG CAGGCATTTC AGGACCATCA TCCTCCATAG CCATAGCTGG CACCAACCAC 
CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TTATAGTCAC CACCGCAGCT 
GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGATT TTCCCTTTGT TGGCCAGGAG 
CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC 
CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC 
CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC 
CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC 
ATGGATGGGC AGGTATTGCA ACCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC 
CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT 
TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT 
TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG 
AACCCTTTAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT 
GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC 
TCGGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA 
ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT 
GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT 
ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGGTGC CACAGCTACT TAACCCTCTA 
CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA 
CTGACTCATC TACAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAACAG 
CAGCAACTTC TTCAGGGGTA CCAGAACCTC CAGGCATTTC AAGGACAGTC CGCAATTCCT 
TGCCCAGCTA ACAATAACCC CATGGCTTGT CTGTTTCAGA ACTTTCAGGT GGAGCCGGAG 
AAGTTGAAGA CACTAACAGA AGGTTTAGAA GCCTACAGTC GAGCCCGGAA AAGGAACAGA 
AAAAGTGGAA AGCTAAATAA CCATTTAGAA GCTGCTATTC ATGAGGCCAT GAGTGAACTG 
GACAAAATGT CTGGGACTGT TCACCAAATC CCACAGGGAG ACAGACAAAT GAGACCCCCC 
AAACCCAAGA GGAGGAAGAT CTCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014587943.1
CDS	915..4904
Translation	MNGGKECDGGDKDGGLPAVQVPVGWQRRVDQNGVLYVSPSGSLLSCLEQVKTYLLTDGTCKCGLECPLILPKVFNFDPGAAVKQRTAEDVKADEDVTKLCIHKRKIIAVATLHKSMEAPHPSLVLTSPGGGTNATPVVPSRAATPRSVRNKSHEGITNSVMPECKNPFKLMIGSSNAMGRLYVQELPGSQQQELHPVYPRQRLGSSEHGQKSPFRGSHGGMPSPASSGSQIYGDGSISPRTDPLGSPDVFTRNNPGFHGAPNSSPIHLSRTPLSPPSVMLHGSPVQSSCAMAGRTNIPLSPTLTTKSPVMKKPMCNFSSNMEIPRAMFHHKPPQGPPPPPPPSCALQKKPLTSEKDPLGILDPIPSKPVNQNPVIINPTSFHSNVHSQVPVMNVSMPPAVVPLPSNLPLPTVKPGHMNHGSHVQRVQHSASTSLSPSPVTSPVHMMGTGIGRIEASPQRSRSSSTSSDHGNFMMPPLGPQATCSGIKVPPRSPRSTIGSPRPSMPSSPSTKSDGHHQYKDIPNPLIAGMSNVLNTPSSAAFPTASAGSGSVKSQPGLLGMPLNQILNQHNAASFPASSLLSAAAKAQLANQNKLAGNNNSSSSNSGAVAGSGNTEGHSTLNTMFPPTANMLLPTGEGQSGRAALRDKLMSQQKDSLRKRKQPPTTVLSLLRQSQMDSSAVPKPGPDLLRKQGQGSFPISSMSQLLQSMSCQSSHLSSNSAPGCGGSNTALPCSANQLHFTDPNMNSSVLQNSLTQNIPLRGEAVHCHNANTNFVHSNSPVPNHHLAGLINQIQASGNCGMLSQSGMALGNSLHPNPPQSRISTSSTPVIPNSIVSSYNQTSSEAGISGPSSSIAIAGTNHPAITKTTSVLQDGVIVTTAAGNPLQSQLPIGSDFPFVGQEHALHFPSNSTSNNHLPHPLNPSLLSSLPISLPVNQQHLLNQNLLNILQPSAGEGKSEINLHPLGFLNPNVNAALAFLSGDMDGQVLQPVHFQLLAALLQNQAQAAAMLPLPSFNLTISDLLQQQNTPLPSLTQMTAPPDHLPSNQSENSRAETLLTSPLGNPLPSFAGSDTTFNPLFLPAVTGASGLMALNPQLLGGVLNSASANTANHPEVSIATSSQATTTTTTTSSAVAALTVSTLGGTAVVSMAETLLNISNNAGNTPGPAKLNSNSVVPQLLNPLLGTGLLGDMSSINNTLNNHQLTHLQSLLNNNQMFPPNQQQQQLLQGYQNLQAFQGQSAIPCPANNNPMACLFQNFQVEPEKLKTLTEGLEAYSRARKRNRKSGKLNNHLEAAIHEAMSELDKMSGTVHQIPQGDRQMRPPKPKRRKISR

Target ORF information:

RefSeq Version	XM_014732457.1
Organism	Equus caballus(horse)
Definition	Equus caballus methyl-CpG binding domain protein 5 (MBD5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014732457.1

ORF Insert Sequence:

ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTGTACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGACGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATCAT TGCAGTGGCA ACACTTCATA AAAGCATGGA AGCTCCACAT 
CCTTCACTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA 
CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
ATGCCCAGCC CAGCGTCGTC AGGTTCCCAG ATATATGGAG ATGGCTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAGTAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACCAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAAGTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACTTCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACTAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA 
TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG 
GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAATAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA CAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGCGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA 
CAGCCACCTA CCACAGTGTT GAGTTTGCTC AGACAGTCGC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCCGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTGCC 
CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAATA TGAACTCCAG TGTTCTTCAG AATTCACTGA CACAGAACAT ACCTTTAAGA 
GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC 
CCGAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG 
CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA 
ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA 
AGTTCTGAAG CAGGCATTTC AGGACCATCA TCCTCCATAG CCATAGCTGG CACCAACCAC 
CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TTATAGTCAC CACCGCAGCT 
GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGATT TTCCCTTTGT TGGCCAGGAG 
CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC 
CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC 
CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC 
CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC 
ATGGATGGGC AGGTATTGCA ACCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC 
CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT 
TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT 
TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG 
AACCCTTTAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT 
GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC 
TCGGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA 
ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT 
GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT 
ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGGTGC CACAGCTACT TAACCCTCTA 
CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA 
CTGACTCATC TACAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAACAG 
CAGCAACTTC TTCAGGGGTA CCAGAACCTC CAGGCATTTC AAGGACAGTC CGCAATTCCT 
TGCCCAGCTA ACAATAACCC CATGGCTTGT CTGTTTCAGA ACTTTCAGGT GGAGCCGGAG 
AAGTTGAAGA CACTAACAGA AGGTTTAGAA GCCTACAGTC GAGCCCGGAA AAGGAACAGA 
AAAAGTGGAA AGCTAAATAA CCATTTAGAA GCTGCTATTC ATGAGGCCAT GAGTGAACTG 
GACAAAATGT CTGGGACTGT TCACCAAATC CCACAGGGAG ACAGACAAAT GAGACCCCCC 
AAACCCAAGA GGAGGAAGAT CTCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD5 ( XM_023622555.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023478323.1 Equus caballus methyl-CpG binding domain protein 5 (MBD5), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHb52493
Clone ID Related Accession (Same CDS sequence)	XM_023622555.1
Accession Version	XM_023622555.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	methyl-CpG-binding domain protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTGTACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGACGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATCAT TGCAGTGGCA ACACTTCATA AAAGCATGGA AGCTCCACAT 
CCTTCACTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA 
CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
ATGCCCAGCC CAGCGTCGTC AGGTTCCCAG ATATATGGAG ATGGCTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAGTAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACCAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAAGTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACTTCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACTAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA 
TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG 
GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAATAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA CAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGCGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA 
CAGCCACCTA CCACAGTGTT GAGTTTGCTC AGACAGTCGC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCCGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTGCC 
CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAATA TGAACTCCAG TGTTCTTCAG AATTCACTGA CACAGAACAT ACCTTTAAGA 
GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC 
CCGAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG 
CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA 
ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA 
AGTTCTGAAG CAGGCATTTC AGGACCATCA TCCTCCATAG CCATAGCTGG CACCAACCAC 
CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TTATAGTCAC CACCGCAGCT 
GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGATT TTCCCTTTGT TGGCCAGGAG 
CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC 
CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC 
CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GTGACATGTC CTCAATAAAC 
AATACTTTGA ATAACCATCA ACTGACTCAT CTACAGTCGC TGTTAAACAA CAATCAGATG 
TTTCCTCCAA ATCAGCAACA GCAGCAACTT CTTCAGGGGT ACCAGAACCT CCAGGCATTT 
CAAGGACAGT CCGCAATTCC TTGCCCAGCT AACAATAACC CCATGGCTTG TCTGTTTCAG 
AACTTTCAGG TGAGGATGCA GGAAGATGCA ACTCTCCTAA ACAAAAGAAT AAGCACTCAG 
CCGGGGCTCA CAGCACTTCC TGAGAATCCG AACACTACAC TTCCGCCTTT CCAAGATACA 
TCTTGTGAGT TGCAACCGAG GATTGACCCA TCTCTTGGTC AGCAGGTGAA GGATGGCCTT 
GTTATGGGTG GCCAAGGTGA TGCTTCTGTA GATGCCATTT ACAAGGCAGT TGTTGATGCA 
GCCAGCAAAG GAATGCAGGT TGTCATCACC ACTGCAGTCA ACAGTACAAC TCAGATCAGC 
CCCATTCCAG CTCTGAGTGC CATGAGTGCC TTCACTGCTT CGATTGGTGA CCCGTTAAGT 
CTCCCCAGTG CTGTCAGTGC GGTCATTCAT GGACGAAACA TGGGCAGTGT CGATCATGAT 
GGTAGGCTGA GGAATGCAAG AGGGGCCCGG CTGCCCAGGA ATCTGGACCA TGGGAAAAAC 
TCAAATGAAG GAGATGGGTT TGAATATTTC AAGGCAGCAA GTTGCCACAC ATCCAAAAAA 
CAGTGGGATG GGGAGCAAAG CCCTGGAGGG GAACGAAACA GGTGGAAATG CGAGGAATTT 
TTAGATCATC CAGGCCATAT CCACAGTAGT CCTTGTCATG AAAGGTCCAA TAATGTCTCT 
ACACTGCCAT TTCTGCCTGG GGAACAGCAC CCAATACTGT TACCACCAAG AAACTGTCAA 
GGGGATAAAA TTCTGGAGGA AAATTTCAGG TATAATAACT ACAAAAGAAC TATGATGACT 
TTTAAGGAGA GACTAGAGAA CACTGTGGAA AGATGTGCAC ACATTAATGG GAACAGACCT 
CGACAGAGTC GGGGCTTTGG AGAGCTGCTG AGTACCACAA GGCAAGACCT CGTCCTAGAG 
GAGCAGTCTC CAAGCTCCTC AAATAGTTTG GAAAGTTCTC TGGTCAAAGA CTACATCCAT 
TATAATGGAG ACTTTAATGC CAAAAGCATT AATGGGTGTG TGCCTAGCCC TTCAGACGCT 
AAAAGCATTA GTAGTGAAGA TGACCTAAGG AATCCAGACT CCCCCTCTTC AAATGAGTTG 
ATACATTATA GGCCAAGGAC GTTCAACGTT GGCGACTTGG TCTGGGGCCA AATCAAAGGA 
CTTACTTCCT GGCCTGGAAA ATTAGTAAGA GAAGACGACG TTCACAATTC ATGTCAACAA 
AGCCCCGAGG AAGGGAAGGT GGAGCCGGAG AAGTTGAAGA CACTAACAGA AGGTTTAGAA 
GCCTACAGTC GAGCCCGGAA AAGGAACAGA AAAAGTGGAA AGCTAAATAA CCATTTAGAA 
GCTGCTATTC ATGAGGCCAT GAGTGAACTG GACAAAATGT CTGGGACTGT TCACCAAATC 
CCACAGGGAG ACAGACAAAT GAGACCCCCC AAACCCAAGA GGAGGAAGAT CTCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023478323.1
CDS	1722..6221
Translation	MNGGKECDGGDKDGGLPAVQVPVGWQRRVDQNGVLYVSPSGSLLSCLEQVKTYLLTDGTCKCGLECPLILPKVFNFDPGAAVKQRTAEDVKADEDVTKLCIHKRKIIAVATLHKSMEAPHPSLVLTSPGGGTNATPVVPSRAATPRSVRNKSHEGITNSVMPECKNPFKLMIGSSNAMGRLYVQELPGSQQQELHPVYPRQRLGSSEHGQKSPFRGSHGGMPSPASSGSQIYGDGSISPRTDPLGSPDVFTRNNPGFHGAPNSSPIHLSRTPLSPPSVMLHGSPVQSSCAMAGRTNIPLSPTLTTKSPVMKKPMCNFSSNMEIPRAMFHHKPPQGPPPPPPPSCALQKKPLTSEKDPLGILDPIPSKPVNQNPVIINPTSFHSNVHSQVPVMNVSMPPAVVPLPSNLPLPTVKPGHMNHGSHVQRVQHSASTSLSPSPVTSPVHMMGTGIGRIEASPQRSRSSSTSSDHGNFMMPPLGPQATCSGIKVPPRSPRSTIGSPRPSMPSSPSTKSDGHHQYKDIPNPLIAGMSNVLNTPSSAAFPTASAGSGSVKSQPGLLGMPLNQILNQHNAASFPASSLLSAAAKAQLANQNKLAGNNNSSSSNSGAVAGSGNTEGHSTLNTMFPPTANMLLPTGEGQSGRAALRDKLMSQQKDSLRKRKQPPTTVLSLLRQSQMDSSAVPKPGPDLLRKQGQGSFPISSMSQLLQSMSCQSSHLSSNSAPGCGGSNTALPCSANQLHFTDPNMNSSVLQNSLTQNIPLRGEAVHCHNANTNFVHSNSPVPNHHLAGLINQIQASGNCGMLSQSGMALGNSLHPNPPQSRISTSSTPVIPNSIVSSYNQTSSEAGISGPSSSIAIAGTNHPAITKTTSVLQDGVIVTTAAGNPLQSQLPIGSDFPFVGQEHALHFPSNSTSNNHLPHPLNPSLLSSLPISLPVNQQHLLNQNLLNILQPSAGEGDMSSINNTLNNHQLTHLQSLLNNNQMFPPNQQQQQLLQGYQNLQAFQGQSAIPCPANNNPMACLFQNFQVRMQEDATLLNKRISTQPGLTALPENPNTTLPPFQDTSCELQPRIDPSLGQQVKDGLVMGGQGDASVDAIYKAVVDAASKGMQVVITTAVNSTTQISPIPALSAMSAFTASIGDPLSLPSAVSAVIHGRNMGSVDHDGRLRNARGARLPRNLDHGKNSNEGDGFEYFKAASCHTSKKQWDGEQSPGGERNRWKCEEFLDHPGHIHSSPCHERSNNVSTLPFLPGEQHPILLPPRNCQGDKILEENFRYNNYKRTMMTFKERLENTVERCAHINGNRPRQSRGFGELLSTTRQDLVLEEQSPSSSNSLESSLVKDYIHYNGDFNAKSINGCVPSPSDAKSISSEDDLRNPDSPSSNELIHYRPRTFNVGDLVWGQIKGLTSWPGKLVREDDVHNSCQQSPEEGKVEPEKLKTLTEGLEAYSRARKRNRKSGKLNNHLEAAIHEAMSELDKMSGTVHQIPQGDRQMRPPKPKRRKISR

Target ORF information:

RefSeq Version	XM_023622555.1
Organism	Equus caballus(horse)
Definition	Equus caballus methyl-CpG binding domain protein 5 (MBD5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023622555.1

ORF Insert Sequence:

ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTGTACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGACGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATCAT TGCAGTGGCA ACACTTCATA AAAGCATGGA AGCTCCACAT 
CCTTCACTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA 
CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
ATGCCCAGCC CAGCGTCGTC AGGTTCCCAG ATATATGGAG ATGGCTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAGTAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACCAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAAGTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACTTCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACTAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA 
TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG 
GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAATAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA CAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGCGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA 
CAGCCACCTA CCACAGTGTT GAGTTTGCTC AGACAGTCGC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCCGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTGCC 
CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAATA TGAACTCCAG TGTTCTTCAG AATTCACTGA CACAGAACAT ACCTTTAAGA 
GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC 
CCGAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG 
CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA 
ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA 
AGTTCTGAAG CAGGCATTTC AGGACCATCA TCCTCCATAG CCATAGCTGG CACCAACCAC 
CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TTATAGTCAC CACCGCAGCT 
GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGATT TTCCCTTTGT TGGCCAGGAG 
CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC 
CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC 
CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GTGACATGTC CTCAATAAAC 
AATACTTTGA ATAACCATCA ACTGACTCAT CTACAGTCGC TGTTAAACAA CAATCAGATG 
TTTCCTCCAA ATCAGCAACA GCAGCAACTT CTTCAGGGGT ACCAGAACCT CCAGGCATTT 
CAAGGACAGT CCGCAATTCC TTGCCCAGCT AACAATAACC CCATGGCTTG TCTGTTTCAG 
AACTTTCAGG TGAGGATGCA GGAAGATGCA ACTCTCCTAA ACAAAAGAAT AAGCACTCAG 
CCGGGGCTCA CAGCACTTCC TGAGAATCCG AACACTACAC TTCCGCCTTT CCAAGATACA 
TCTTGTGAGT TGCAACCGAG GATTGACCCA TCTCTTGGTC AGCAGGTGAA GGATGGCCTT 
GTTATGGGTG GCCAAGGTGA TGCTTCTGTA GATGCCATTT ACAAGGCAGT TGTTGATGCA 
GCCAGCAAAG GAATGCAGGT TGTCATCACC ACTGCAGTCA ACAGTACAAC TCAGATCAGC 
CCCATTCCAG CTCTGAGTGC CATGAGTGCC TTCACTGCTT CGATTGGTGA CCCGTTAAGT 
CTCCCCAGTG CTGTCAGTGC GGTCATTCAT GGACGAAACA TGGGCAGTGT CGATCATGAT 
GGTAGGCTGA GGAATGCAAG AGGGGCCCGG CTGCCCAGGA ATCTGGACCA TGGGAAAAAC 
TCAAATGAAG GAGATGGGTT TGAATATTTC AAGGCAGCAA GTTGCCACAC ATCCAAAAAA 
CAGTGGGATG GGGAGCAAAG CCCTGGAGGG GAACGAAACA GGTGGAAATG CGAGGAATTT 
TTAGATCATC CAGGCCATAT CCACAGTAGT CCTTGTCATG AAAGGTCCAA TAATGTCTCT 
ACACTGCCAT TTCTGCCTGG GGAACAGCAC CCAATACTGT TACCACCAAG AAACTGTCAA 
GGGGATAAAA TTCTGGAGGA AAATTTCAGG TATAATAACT ACAAAAGAAC TATGATGACT 
TTTAAGGAGA GACTAGAGAA CACTGTGGAA AGATGTGCAC ACATTAATGG GAACAGACCT 
CGACAGAGTC GGGGCTTTGG AGAGCTGCTG AGTACCACAA GGCAAGACCT CGTCCTAGAG 
GAGCAGTCTC CAAGCTCCTC AAATAGTTTG GAAAGTTCTC TGGTCAAAGA CTACATCCAT 
TATAATGGAG ACTTTAATGC CAAAAGCATT AATGGGTGTG TGCCTAGCCC TTCAGACGCT 
AAAAGCATTA GTAGTGAAGA TGACCTAAGG AATCCAGACT CCCCCTCTTC AAATGAGTTG 
ATACATTATA GGCCAAGGAC GTTCAACGTT GGCGACTTGG TCTGGGGCCA AATCAAAGGA 
CTTACTTCCT GGCCTGGAAA ATTAGTAAGA GAAGACGACG TTCACAATTC ATGTCAACAA 
AGCCCCGAGG AAGGGAAGGT GGAGCCGGAG AAGTTGAAGA CACTAACAGA AGGTTTAGAA 
GCCTACAGTC GAGCCCGGAA AAGGAACAGA AAAAGTGGAA AGCTAAATAA CCATTTAGAA 
GCTGCTATTC ATGAGGCCAT GAGTGAACTG GACAAAATGT CTGGGACTGT TCACCAAATC 
CCACAGGGAG ACAGACAAAT GAGACCCCCC AAACCCAAGA GGAGGAAGAT CTCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD5 ( XM_014732456.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_014587942.1 Equus caballus methyl-CpG binding domain protein 5 (MBD5), transcript variant X2, mRNA.

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CloneID	OHb08212
Clone ID Related Accession (Same CDS sequence)	XM_014732456.1 , XM_023622554.1 , XM_005601478.2 , XM_005601478.3 , XM_014732456.2
Accession Version	XM_014732456.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	methyl-CpG-binding domain protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867384.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTGTACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGACGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATCAT TGCAGTGGCA ACACTTCATA AAAGCATGGA AGCTCCACAT 
CCTTCACTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA 
CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
ATGCCCAGCC CAGCGTCGTC AGGTTCCCAG ATATATGGAG ATGGCTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAGTAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACCAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAAGTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACTTCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACTAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA 
TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG 
GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAATAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA CAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGCGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA 
CAGCCACCTA CCACAGTGTT GAGTTTGCTC AGACAGTCGC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCCGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTGCC 
CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAATA TGAACTCCAG TGTTCTTCAG AATTCACTGA CACAGAACAT ACCTTTAAGA 
GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC 
CCGAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG 
CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA 
ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA 
AGTTCTGAAG CAGGCATTTC AGGACCATCA TCCTCCATAG CCATAGCTGG CACCAACCAC 
CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TTATAGTCAC CACCGCAGCT 
GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGATT TTCCCTTTGT TGGCCAGGAG 
CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC 
CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC 
CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC 
CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC 
ATGGATGGGC AGGTATTGCA ACCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC 
CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT 
TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT 
TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG 
AACCCTTTAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT 
GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC 
TCGGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA 
ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT 
GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT 
ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGGTGC CACAGCTACT TAACCCTCTA 
CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA 
CTGACTCATC TACAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAACAG 
CAGCAACTTC TTCAGGGGTA CCAGAACCTC CAGGCATTTC AAGGACAGTC CGCAATTCCT 
TGCCCAGCTA ACAATAACCC CATGGCTTGT CTGTTTCAGA ACTTTCAGGT GAGGATGCAG 
GAAGATGCAA CTCTCCTAAA CAAAAGAATA AGCACTCAGC CGGGGCTCAC AGCACTTCCT 
GAGAATCCGA ACACTACACT TCCGCCTTTC CAAGATACAT CTTGTGAGTT GCAACCGAGG 
ATTGACCCAT CTCTTGGTCA GCAGGTGAAG GATGGCCTTG TTATGGGTGG CCAAGGTGAT 
GCTTCTGTAG ATGCCATTTA CAAGGCAGTT GTTGATGCAG CCAGCAAAGG AATGCAGGTT 
GTCATCACCA CTGCAGTCAA CAGTACAACT CAGATCAGCC CCATTCCAGC TCTGAGTGCC 
ATGAGTGCCT TCACTGCTTC GATTGGTGAC CCGTTAAGTC TCCCCAGTGC TGTCAGTGCG 
GTCATTCATG GACGAAACAT GGGCAGTGTC GATCATGATG GTAGGCTGAG GAATGCAAGA 
GGGGCCCGGC TGCCCAGGAA TCTGGACCAT GGGAAAAACT CAAATGAAGG AGATGGGTTT 
GAATATTTCA AGGCAGCAAG TTGCCACACA TCCAAAAAAC AGTGGGATGG GGAGCAAAGC 
CCTGGAGGGG AACGAAACAG GTGGAAATGC GAGGAATTTT TAGATCATCC AGGCCATATC 
CACAGTAGTC CTTGTCATGA AAGGTCCAAT AATGTCTCTA CACTGCCATT TCTGCCTGGG 
GAACAGCACC CAATACTGTT ACCACCAAGA AACTGTCAAG GGGATAAAAT TCTGGAGGAA 
AATTTCAGGT ATAATAACTA CAAAAGAACT ATGATGACTT TTAAGGAGAG ACTAGAGAAC 
ACTGTGGAAA GATGTGCACA CATTAATGGG AACAGACCTC GACAGAGTCG GGGCTTTGGA 
GAGCTGCTGA GTACCACAAG GCAAGACCTC GTCCTAGAGG AGCAGTCTCC AAGCTCCTCA 
AATAGTTTGG AAAGTTCTCT GGTCAAAGAC TACATCCATT ATAATGGAGA CTTTAATGCC 
AAAAGCATTA ATGGGTGTGT GCCTAGCCCT TCAGACGCTA AAAGCATTAG TAGTGAAGAT 
GACCTAAGGA ATCCAGACTC CCCCTCTTCA AATGAGTTGA TACATTATAG GCCAAGGACG 
TTCAACGTTG GCGACTTGGT CTGGGGCCAA ATCAAAGGAC TTACTTCCTG GCCTGGAAAA 
TTAGTAAGAG AAGACGACGT TCACAATTCA TGTCAACAAA GCCCCGAGGA AGGGAAGGTG 
GAGCCGGAGA AGTTGAAGAC ACTAACAGAA GGTTTAGAAG CCTACAGTCG AGCCCGGAAA 
AGGAACAGAA AAAGTGGAAA GCTAAATAAC CATTTAGAAG CTGCTATTCA TGAGGCCATG 
AGTGAACTGG ACAAAATGTC TGGGACTGTT CACCAAATCC CACAGGGAGA CAGACAAATG 
AGACCCCCCA AACCCAAGAG GAGGAAGATC TCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014587942.1
CDS	742..5940
Translation	MNGGKECDGGDKDGGLPAVQVPVGWQRRVDQNGVLYVSPSGSLLSCLEQVKTYLLTDGTCKCGLECPLILPKVFNFDPGAAVKQRTAEDVKADEDVTKLCIHKRKIIAVATLHKSMEAPHPSLVLTSPGGGTNATPVVPSRAATPRSVRNKSHEGITNSVMPECKNPFKLMIGSSNAMGRLYVQELPGSQQQELHPVYPRQRLGSSEHGQKSPFRGSHGGMPSPASSGSQIYGDGSISPRTDPLGSPDVFTRNNPGFHGAPNSSPIHLSRTPLSPPSVMLHGSPVQSSCAMAGRTNIPLSPTLTTKSPVMKKPMCNFSSNMEIPRAMFHHKPPQGPPPPPPPSCALQKKPLTSEKDPLGILDPIPSKPVNQNPVIINPTSFHSNVHSQVPVMNVSMPPAVVPLPSNLPLPTVKPGHMNHGSHVQRVQHSASTSLSPSPVTSPVHMMGTGIGRIEASPQRSRSSSTSSDHGNFMMPPLGPQATCSGIKVPPRSPRSTIGSPRPSMPSSPSTKSDGHHQYKDIPNPLIAGMSNVLNTPSSAAFPTASAGSGSVKSQPGLLGMPLNQILNQHNAASFPASSLLSAAAKAQLANQNKLAGNNNSSSSNSGAVAGSGNTEGHSTLNTMFPPTANMLLPTGEGQSGRAALRDKLMSQQKDSLRKRKQPPTTVLSLLRQSQMDSSAVPKPGPDLLRKQGQGSFPISSMSQLLQSMSCQSSHLSSNSAPGCGGSNTALPCSANQLHFTDPNMNSSVLQNSLTQNIPLRGEAVHCHNANTNFVHSNSPVPNHHLAGLINQIQASGNCGMLSQSGMALGNSLHPNPPQSRISTSSTPVIPNSIVSSYNQTSSEAGISGPSSSIAIAGTNHPAITKTTSVLQDGVIVTTAAGNPLQSQLPIGSDFPFVGQEHALHFPSNSTSNNHLPHPLNPSLLSSLPISLPVNQQHLLNQNLLNILQPSAGEGKSEINLHPLGFLNPNVNAALAFLSGDMDGQVLQPVHFQLLAALLQNQAQAAAMLPLPSFNLTISDLLQQQNTPLPSLTQMTAPPDHLPSNQSENSRAETLLTSPLGNPLPSFAGSDTTFNPLFLPAVTGASGLMALNPQLLGGVLNSASANTANHPEVSIATSSQATTTTTTTSSAVAALTVSTLGGTAVVSMAETLLNISNNAGNTPGPAKLNSNSVVPQLLNPLLGTGLLGDMSSINNTLNNHQLTHLQSLLNNNQMFPPNQQQQQLLQGYQNLQAFQGQSAIPCPANNNPMACLFQNFQVRMQEDATLLNKRISTQPGLTALPENPNTTLPPFQDTSCELQPRIDPSLGQQVKDGLVMGGQGDASVDAIYKAVVDAASKGMQVVITTAVNSTTQISPIPALSAMSAFTASIGDPLSLPSAVSAVIHGRNMGSVDHDGRLRNARGARLPRNLDHGKNSNEGDGFEYFKAASCHTSKKQWDGEQSPGGERNRWKCEEFLDHPGHIHSSPCHERSNNVSTLPFLPGEQHPILLPPRNCQGDKILEENFRYNNYKRTMMTFKERLENTVERCAHINGNRPRQSRGFGELLSTTRQDLVLEEQSPSSSNSLESSLVKDYIHYNGDFNAKSINGCVPSPSDAKSISSEDDLRNPDSPSSNELIHYRPRTFNVGDLVWGQIKGLTSWPGKLVREDDVHNSCQQSPEEGKVEPEKLKTLTEGLEAYSRARKRNRKSGKLNNHLEAAIHEAMSELDKMSGTVHQIPQGDRQMRPPKPKRRKISR

Target ORF information:

RefSeq Version	XM_014732456.1
Organism	Equus caballus(horse)
Definition	Equus caballus methyl-CpG binding domain protein 5 (MBD5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014732456.1

ORF Insert Sequence:

ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTGTACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGACGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATCAT TGCAGTGGCA ACACTTCATA AAAGCATGGA AGCTCCACAT 
CCTTCACTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA 
CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
ATGCCCAGCC CAGCGTCGTC AGGTTCCCAG ATATATGGAG ATGGCTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAGTAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACCAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAAGTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACTTCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACTAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA 
TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG 
GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAATAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA CAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGCGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA 
CAGCCACCTA CCACAGTGTT GAGTTTGCTC AGACAGTCGC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCCGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTGCC 
CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAATA TGAACTCCAG TGTTCTTCAG AATTCACTGA CACAGAACAT ACCTTTAAGA 
GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC 
CCGAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG 
CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA 
ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA 
AGTTCTGAAG CAGGCATTTC AGGACCATCA TCCTCCATAG CCATAGCTGG CACCAACCAC 
CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TTATAGTCAC CACCGCAGCT 
GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGATT TTCCCTTTGT TGGCCAGGAG 
CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC 
CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC 
CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC 
CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC 
ATGGATGGGC AGGTATTGCA ACCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC 
CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT 
TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT 
TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG 
AACCCTTTAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT 
GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC 
TCGGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA 
ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT 
GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT 
ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGGTGC CACAGCTACT TAACCCTCTA 
CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA 
CTGACTCATC TACAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAACAG 
CAGCAACTTC TTCAGGGGTA CCAGAACCTC CAGGCATTTC AAGGACAGTC CGCAATTCCT 
TGCCCAGCTA ACAATAACCC CATGGCTTGT CTGTTTCAGA ACTTTCAGGT GAGGATGCAG 
GAAGATGCAA CTCTCCTAAA CAAAAGAATA AGCACTCAGC CGGGGCTCAC AGCACTTCCT 
GAGAATCCGA ACACTACACT TCCGCCTTTC CAAGATACAT CTTGTGAGTT GCAACCGAGG 
ATTGACCCAT CTCTTGGTCA GCAGGTGAAG GATGGCCTTG TTATGGGTGG CCAAGGTGAT 
GCTTCTGTAG ATGCCATTTA CAAGGCAGTT GTTGATGCAG CCAGCAAAGG AATGCAGGTT 
GTCATCACCA CTGCAGTCAA CAGTACAACT CAGATCAGCC CCATTCCAGC TCTGAGTGCC 
ATGAGTGCCT TCACTGCTTC GATTGGTGAC CCGTTAAGTC TCCCCAGTGC TGTCAGTGCG 
GTCATTCATG GACGAAACAT GGGCAGTGTC GATCATGATG GTAGGCTGAG GAATGCAAGA 
GGGGCCCGGC TGCCCAGGAA TCTGGACCAT GGGAAAAACT CAAATGAAGG AGATGGGTTT 
GAATATTTCA AGGCAGCAAG TTGCCACACA TCCAAAAAAC AGTGGGATGG GGAGCAAAGC 
CCTGGAGGGG AACGAAACAG GTGGAAATGC GAGGAATTTT TAGATCATCC AGGCCATATC 
CACAGTAGTC CTTGTCATGA AAGGTCCAAT AATGTCTCTA CACTGCCATT TCTGCCTGGG 
GAACAGCACC CAATACTGTT ACCACCAAGA AACTGTCAAG GGGATAAAAT TCTGGAGGAA 
AATTTCAGGT ATAATAACTA CAAAAGAACT ATGATGACTT TTAAGGAGAG ACTAGAGAAC 
ACTGTGGAAA GATGTGCACA CATTAATGGG AACAGACCTC GACAGAGTCG GGGCTTTGGA 
GAGCTGCTGA GTACCACAAG GCAAGACCTC GTCCTAGAGG AGCAGTCTCC AAGCTCCTCA 
AATAGTTTGG AAAGTTCTCT GGTCAAAGAC TACATCCATT ATAATGGAGA CTTTAATGCC 
AAAAGCATTA ATGGGTGTGT GCCTAGCCCT TCAGACGCTA AAAGCATTAG TAGTGAAGAT 
GACCTAAGGA ATCCAGACTC CCCCTCTTCA AATGAGTTGA TACATTATAG GCCAAGGACG 
TTCAACGTTG GCGACTTGGT CTGGGGCCAA ATCAAAGGAC TTACTTCCTG GCCTGGAAAA 
TTAGTAAGAG AAGACGACGT TCACAATTCA TGTCAACAAA GCCCCGAGGA AGGGAAGGTG 
GAGCCGGAGA AGTTGAAGAC ACTAACAGAA GGTTTAGAAG CCTACAGTCG AGCCCGGAAA 
AGGAACAGAA AAAGTGGAAA GCTAAATAAC CATTTAGAAG CTGCTATTCA TGAGGCCATG 
AGTGAACTGG ACAAAATGTC TGGGACTGTT CACCAAATCC CACAGGGAGA CAGACAAATG 
AGACCCCCCA AACCCAAGAG GAGGAAGATC TCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD5 ( XM_023622554.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023478322.1 Equus caballus methyl-CpG binding domain protein 5 (MBD5), transcript variant X2, mRNA.

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CloneID	OHb08212
Clone ID Related Accession (Same CDS sequence)	XM_014732456.1 , XM_023622554.1 , XM_005601478.2 , XM_005601478.3 , XM_014732456.2
Accession Version	XM_023622554.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	methyl-CpG-binding domain protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTGTACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGACGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATCAT TGCAGTGGCA ACACTTCATA AAAGCATGGA AGCTCCACAT 
CCTTCACTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA 
CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
ATGCCCAGCC CAGCGTCGTC AGGTTCCCAG ATATATGGAG ATGGCTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAGTAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACCAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAAGTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACTTCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACTAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA 
TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG 
GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAATAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA CAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGCGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA 
CAGCCACCTA CCACAGTGTT GAGTTTGCTC AGACAGTCGC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCCGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTGCC 
CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAATA TGAACTCCAG TGTTCTTCAG AATTCACTGA CACAGAACAT ACCTTTAAGA 
GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC 
CCGAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG 
CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA 
ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA 
AGTTCTGAAG CAGGCATTTC AGGACCATCA TCCTCCATAG CCATAGCTGG CACCAACCAC 
CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TTATAGTCAC CACCGCAGCT 
GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGATT TTCCCTTTGT TGGCCAGGAG 
CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC 
CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC 
CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC 
CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC 
ATGGATGGGC AGGTATTGCA ACCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC 
CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT 
TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT 
TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG 
AACCCTTTAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT 
GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC 
TCGGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA 
ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT 
GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT 
ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGGTGC CACAGCTACT TAACCCTCTA 
CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA 
CTGACTCATC TACAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAACAG 
CAGCAACTTC TTCAGGGGTA CCAGAACCTC CAGGCATTTC AAGGACAGTC CGCAATTCCT 
TGCCCAGCTA ACAATAACCC CATGGCTTGT CTGTTTCAGA ACTTTCAGGT GAGGATGCAG 
GAAGATGCAA CTCTCCTAAA CAAAAGAATA AGCACTCAGC CGGGGCTCAC AGCACTTCCT 
GAGAATCCGA ACACTACACT TCCGCCTTTC CAAGATACAT CTTGTGAGTT GCAACCGAGG 
ATTGACCCAT CTCTTGGTCA GCAGGTGAAG GATGGCCTTG TTATGGGTGG CCAAGGTGAT 
GCTTCTGTAG ATGCCATTTA CAAGGCAGTT GTTGATGCAG CCAGCAAAGG AATGCAGGTT 
GTCATCACCA CTGCAGTCAA CAGTACAACT CAGATCAGCC CCATTCCAGC TCTGAGTGCC 
ATGAGTGCCT TCACTGCTTC GATTGGTGAC CCGTTAAGTC TCCCCAGTGC TGTCAGTGCG 
GTCATTCATG GACGAAACAT GGGCAGTGTC GATCATGATG GTAGGCTGAG GAATGCAAGA 
GGGGCCCGGC TGCCCAGGAA TCTGGACCAT GGGAAAAACT CAAATGAAGG AGATGGGTTT 
GAATATTTCA AGGCAGCAAG TTGCCACACA TCCAAAAAAC AGTGGGATGG GGAGCAAAGC 
CCTGGAGGGG AACGAAACAG GTGGAAATGC GAGGAATTTT TAGATCATCC AGGCCATATC 
CACAGTAGTC CTTGTCATGA AAGGTCCAAT AATGTCTCTA CACTGCCATT TCTGCCTGGG 
GAACAGCACC CAATACTGTT ACCACCAAGA AACTGTCAAG GGGATAAAAT TCTGGAGGAA 
AATTTCAGGT ATAATAACTA CAAAAGAACT ATGATGACTT TTAAGGAGAG ACTAGAGAAC 
ACTGTGGAAA GATGTGCACA CATTAATGGG AACAGACCTC GACAGAGTCG GGGCTTTGGA 
GAGCTGCTGA GTACCACAAG GCAAGACCTC GTCCTAGAGG AGCAGTCTCC AAGCTCCTCA 
AATAGTTTGG AAAGTTCTCT GGTCAAAGAC TACATCCATT ATAATGGAGA CTTTAATGCC 
AAAAGCATTA ATGGGTGTGT GCCTAGCCCT TCAGACGCTA AAAGCATTAG TAGTGAAGAT 
GACCTAAGGA ATCCAGACTC CCCCTCTTCA AATGAGTTGA TACATTATAG GCCAAGGACG 
TTCAACGTTG GCGACTTGGT CTGGGGCCAA ATCAAAGGAC TTACTTCCTG GCCTGGAAAA 
TTAGTAAGAG AAGACGACGT TCACAATTCA TGTCAACAAA GCCCCGAGGA AGGGAAGGTG 
GAGCCGGAGA AGTTGAAGAC ACTAACAGAA GGTTTAGAAG CCTACAGTCG AGCCCGGAAA 
AGGAACAGAA AAAGTGGAAA GCTAAATAAC CATTTAGAAG CTGCTATTCA TGAGGCCATG 
AGTGAACTGG ACAAAATGTC TGGGACTGTT CACCAAATCC CACAGGGAGA CAGACAAATG 
AGACCCCCCA AACCCAAGAG GAGGAAGATC TCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023478322.1
CDS	1947..7145
Translation	MNGGKECDGGDKDGGLPAVQVPVGWQRRVDQNGVLYVSPSGSLLSCLEQVKTYLLTDGTCKCGLECPLILPKVFNFDPGAAVKQRTAEDVKADEDVTKLCIHKRKIIAVATLHKSMEAPHPSLVLTSPGGGTNATPVVPSRAATPRSVRNKSHEGITNSVMPECKNPFKLMIGSSNAMGRLYVQELPGSQQQELHPVYPRQRLGSSEHGQKSPFRGSHGGMPSPASSGSQIYGDGSISPRTDPLGSPDVFTRNNPGFHGAPNSSPIHLSRTPLSPPSVMLHGSPVQSSCAMAGRTNIPLSPTLTTKSPVMKKPMCNFSSNMEIPRAMFHHKPPQGPPPPPPPSCALQKKPLTSEKDPLGILDPIPSKPVNQNPVIINPTSFHSNVHSQVPVMNVSMPPAVVPLPSNLPLPTVKPGHMNHGSHVQRVQHSASTSLSPSPVTSPVHMMGTGIGRIEASPQRSRSSSTSSDHGNFMMPPLGPQATCSGIKVPPRSPRSTIGSPRPSMPSSPSTKSDGHHQYKDIPNPLIAGMSNVLNTPSSAAFPTASAGSGSVKSQPGLLGMPLNQILNQHNAASFPASSLLSAAAKAQLANQNKLAGNNNSSSSNSGAVAGSGNTEGHSTLNTMFPPTANMLLPTGEGQSGRAALRDKLMSQQKDSLRKRKQPPTTVLSLLRQSQMDSSAVPKPGPDLLRKQGQGSFPISSMSQLLQSMSCQSSHLSSNSAPGCGGSNTALPCSANQLHFTDPNMNSSVLQNSLTQNIPLRGEAVHCHNANTNFVHSNSPVPNHHLAGLINQIQASGNCGMLSQSGMALGNSLHPNPPQSRISTSSTPVIPNSIVSSYNQTSSEAGISGPSSSIAIAGTNHPAITKTTSVLQDGVIVTTAAGNPLQSQLPIGSDFPFVGQEHALHFPSNSTSNNHLPHPLNPSLLSSLPISLPVNQQHLLNQNLLNILQPSAGEGKSEINLHPLGFLNPNVNAALAFLSGDMDGQVLQPVHFQLLAALLQNQAQAAAMLPLPSFNLTISDLLQQQNTPLPSLTQMTAPPDHLPSNQSENSRAETLLTSPLGNPLPSFAGSDTTFNPLFLPAVTGASGLMALNPQLLGGVLNSASANTANHPEVSIATSSQATTTTTTTSSAVAALTVSTLGGTAVVSMAETLLNISNNAGNTPGPAKLNSNSVVPQLLNPLLGTGLLGDMSSINNTLNNHQLTHLQSLLNNNQMFPPNQQQQQLLQGYQNLQAFQGQSAIPCPANNNPMACLFQNFQVRMQEDATLLNKRISTQPGLTALPENPNTTLPPFQDTSCELQPRIDPSLGQQVKDGLVMGGQGDASVDAIYKAVVDAASKGMQVVITTAVNSTTQISPIPALSAMSAFTASIGDPLSLPSAVSAVIHGRNMGSVDHDGRLRNARGARLPRNLDHGKNSNEGDGFEYFKAASCHTSKKQWDGEQSPGGERNRWKCEEFLDHPGHIHSSPCHERSNNVSTLPFLPGEQHPILLPPRNCQGDKILEENFRYNNYKRTMMTFKERLENTVERCAHINGNRPRQSRGFGELLSTTRQDLVLEEQSPSSSNSLESSLVKDYIHYNGDFNAKSINGCVPSPSDAKSISSEDDLRNPDSPSSNELIHYRPRTFNVGDLVWGQIKGLTSWPGKLVREDDVHNSCQQSPEEGKVEPEKLKTLTEGLEAYSRARKRNRKSGKLNNHLEAAIHEAMSELDKMSGTVHQIPQGDRQMRPPKPKRRKISR

Target ORF information:

RefSeq Version	XM_023622554.1
Organism	Equus caballus(horse)
Definition	Equus caballus methyl-CpG binding domain protein 5 (MBD5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023622554.1

ORF Insert Sequence:

ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTGTACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGACGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATCAT TGCAGTGGCA ACACTTCATA AAAGCATGGA AGCTCCACAT 
CCTTCACTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA 
CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
ATGCCCAGCC CAGCGTCGTC AGGTTCCCAG ATATATGGAG ATGGCTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAGTAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACCAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAAGTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACTTCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACTAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA 
TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG 
GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAATAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA CAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGCGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA 
CAGCCACCTA CCACAGTGTT GAGTTTGCTC AGACAGTCGC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCCGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTGCC 
CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAATA TGAACTCCAG TGTTCTTCAG AATTCACTGA CACAGAACAT ACCTTTAAGA 
GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC 
CCGAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG 
CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA 
ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA 
AGTTCTGAAG CAGGCATTTC AGGACCATCA TCCTCCATAG CCATAGCTGG CACCAACCAC 
CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TTATAGTCAC CACCGCAGCT 
GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGATT TTCCCTTTGT TGGCCAGGAG 
CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC 
CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC 
CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC 
CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC 
ATGGATGGGC AGGTATTGCA ACCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC 
CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT 
TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT 
TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG 
AACCCTTTAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT 
GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC 
TCGGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA 
ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT 
GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT 
ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGGTGC CACAGCTACT TAACCCTCTA 
CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA 
CTGACTCATC TACAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAACAG 
CAGCAACTTC TTCAGGGGTA CCAGAACCTC CAGGCATTTC AAGGACAGTC CGCAATTCCT 
TGCCCAGCTA ACAATAACCC CATGGCTTGT CTGTTTCAGA ACTTTCAGGT GAGGATGCAG 
GAAGATGCAA CTCTCCTAAA CAAAAGAATA AGCACTCAGC CGGGGCTCAC AGCACTTCCT 
GAGAATCCGA ACACTACACT TCCGCCTTTC CAAGATACAT CTTGTGAGTT GCAACCGAGG 
ATTGACCCAT CTCTTGGTCA GCAGGTGAAG GATGGCCTTG TTATGGGTGG CCAAGGTGAT 
GCTTCTGTAG ATGCCATTTA CAAGGCAGTT GTTGATGCAG CCAGCAAAGG AATGCAGGTT 
GTCATCACCA CTGCAGTCAA CAGTACAACT CAGATCAGCC CCATTCCAGC TCTGAGTGCC 
ATGAGTGCCT TCACTGCTTC GATTGGTGAC CCGTTAAGTC TCCCCAGTGC TGTCAGTGCG 
GTCATTCATG GACGAAACAT GGGCAGTGTC GATCATGATG GTAGGCTGAG GAATGCAAGA 
GGGGCCCGGC TGCCCAGGAA TCTGGACCAT GGGAAAAACT CAAATGAAGG AGATGGGTTT 
GAATATTTCA AGGCAGCAAG TTGCCACACA TCCAAAAAAC AGTGGGATGG GGAGCAAAGC 
CCTGGAGGGG AACGAAACAG GTGGAAATGC GAGGAATTTT TAGATCATCC AGGCCATATC 
CACAGTAGTC CTTGTCATGA AAGGTCCAAT AATGTCTCTA CACTGCCATT TCTGCCTGGG 
GAACAGCACC CAATACTGTT ACCACCAAGA AACTGTCAAG GGGATAAAAT TCTGGAGGAA 
AATTTCAGGT ATAATAACTA CAAAAGAACT ATGATGACTT TTAAGGAGAG ACTAGAGAAC 
ACTGTGGAAA GATGTGCACA CATTAATGGG AACAGACCTC GACAGAGTCG GGGCTTTGGA 
GAGCTGCTGA GTACCACAAG GCAAGACCTC GTCCTAGAGG AGCAGTCTCC AAGCTCCTCA 
AATAGTTTGG AAAGTTCTCT GGTCAAAGAC TACATCCATT ATAATGGAGA CTTTAATGCC 
AAAAGCATTA ATGGGTGTGT GCCTAGCCCT TCAGACGCTA AAAGCATTAG TAGTGAAGAT 
GACCTAAGGA ATCCAGACTC CCCCTCTTCA AATGAGTTGA TACATTATAG GCCAAGGACG 
TTCAACGTTG GCGACTTGGT CTGGGGCCAA ATCAAAGGAC TTACTTCCTG GCCTGGAAAA 
TTAGTAAGAG AAGACGACGT TCACAATTCA TGTCAACAAA GCCCCGAGGA AGGGAAGGTG 
GAGCCGGAGA AGTTGAAGAC ACTAACAGAA GGTTTAGAAG CCTACAGTCG AGCCCGGAAA 
AGGAACAGAA AAAGTGGAAA GCTAAATAAC CATTTAGAAG CTGCTATTCA TGAGGCCATG 
AGTGAACTGG ACAAAATGTC TGGGACTGTT CACCAAATCC CACAGGGAGA CAGACAAATG 
AGACCCCCCA AACCCAAGAG GAGGAAGATC TCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD5 ( XM_005601478.2 ) cDNA ORF clone, Equus caballus(horse) -> XP_005601535.1 Equus caballus methyl-CpG binding domain protein 5 (MBD5), transcript variant X1, mRNA.

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CloneID	OHb08212
Clone ID Related Accession (Same CDS sequence)	XM_014732456.1 , XM_023622554.1 , XM_005601478.2 , XM_005601478.3 , XM_014732456.2
Accession Version	XM_005601478.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	methyl-CpG-binding domain protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867384.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 20, 2015 this sequence version replaced XM_005601478.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTGTACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGACGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATCAT TGCAGTGGCA ACACTTCATA AAAGCATGGA AGCTCCACAT 
CCTTCACTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA 
CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
ATGCCCAGCC CAGCGTCGTC AGGTTCCCAG ATATATGGAG ATGGCTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAGTAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACCAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAAGTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACTTCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACTAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA 
TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG 
GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAATAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA CAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGCGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA 
CAGCCACCTA CCACAGTGTT GAGTTTGCTC AGACAGTCGC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCCGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTGCC 
CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAATA TGAACTCCAG TGTTCTTCAG AATTCACTGA CACAGAACAT ACCTTTAAGA 
GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC 
CCGAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG 
CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA 
ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA 
AGTTCTGAAG CAGGCATTTC AGGACCATCA TCCTCCATAG CCATAGCTGG CACCAACCAC 
CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TTATAGTCAC CACCGCAGCT 
GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGATT TTCCCTTTGT TGGCCAGGAG 
CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC 
CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC 
CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC 
CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC 
ATGGATGGGC AGGTATTGCA ACCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC 
CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT 
TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT 
TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG 
AACCCTTTAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT 
GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC 
TCGGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA 
ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT 
GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT 
ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGGTGC CACAGCTACT TAACCCTCTA 
CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA 
CTGACTCATC TACAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAACAG 
CAGCAACTTC TTCAGGGGTA CCAGAACCTC CAGGCATTTC AAGGACAGTC CGCAATTCCT 
TGCCCAGCTA ACAATAACCC CATGGCTTGT CTGTTTCAGA ACTTTCAGGT GAGGATGCAG 
GAAGATGCAA CTCTCCTAAA CAAAAGAATA AGCACTCAGC CGGGGCTCAC AGCACTTCCT 
GAGAATCCGA ACACTACACT TCCGCCTTTC CAAGATACAT CTTGTGAGTT GCAACCGAGG 
ATTGACCCAT CTCTTGGTCA GCAGGTGAAG GATGGCCTTG TTATGGGTGG CCAAGGTGAT 
GCTTCTGTAG ATGCCATTTA CAAGGCAGTT GTTGATGCAG CCAGCAAAGG AATGCAGGTT 
GTCATCACCA CTGCAGTCAA CAGTACAACT CAGATCAGCC CCATTCCAGC TCTGAGTGCC 
ATGAGTGCCT TCACTGCTTC GATTGGTGAC CCGTTAAGTC TCCCCAGTGC TGTCAGTGCG 
GTCATTCATG GACGAAACAT GGGCAGTGTC GATCATGATG GTAGGCTGAG GAATGCAAGA 
GGGGCCCGGC TGCCCAGGAA TCTGGACCAT GGGAAAAACT CAAATGAAGG AGATGGGTTT 
GAATATTTCA AGGCAGCAAG TTGCCACACA TCCAAAAAAC AGTGGGATGG GGAGCAAAGC 
CCTGGAGGGG AACGAAACAG GTGGAAATGC GAGGAATTTT TAGATCATCC AGGCCATATC 
CACAGTAGTC CTTGTCATGA AAGGTCCAAT AATGTCTCTA CACTGCCATT TCTGCCTGGG 
GAACAGCACC CAATACTGTT ACCACCAAGA AACTGTCAAG GGGATAAAAT TCTGGAGGAA 
AATTTCAGGT ATAATAACTA CAAAAGAACT ATGATGACTT TTAAGGAGAG ACTAGAGAAC 
ACTGTGGAAA GATGTGCACA CATTAATGGG AACAGACCTC GACAGAGTCG GGGCTTTGGA 
GAGCTGCTGA GTACCACAAG GCAAGACCTC GTCCTAGAGG AGCAGTCTCC AAGCTCCTCA 
AATAGTTTGG AAAGTTCTCT GGTCAAAGAC TACATCCATT ATAATGGAGA CTTTAATGCC 
AAAAGCATTA ATGGGTGTGT GCCTAGCCCT TCAGACGCTA AAAGCATTAG TAGTGAAGAT 
GACCTAAGGA ATCCAGACTC CCCCTCTTCA AATGAGTTGA TACATTATAG GCCAAGGACG 
TTCAACGTTG GCGACTTGGT CTGGGGCCAA ATCAAAGGAC TTACTTCCTG GCCTGGAAAA 
TTAGTAAGAG AAGACGACGT TCACAATTCA TGTCAACAAA GCCCCGAGGA AGGGAAGGTG 
GAGCCGGAGA AGTTGAAGAC ACTAACAGAA GGTTTAGAAG CCTACAGTCG AGCCCGGAAA 
AGGAACAGAA AAAGTGGAAA GCTAAATAAC CATTTAGAAG CTGCTATTCA TGAGGCCATG 
AGTGAACTGG ACAAAATGTC TGGGACTGTT CACCAAATCC CACAGGGAGA CAGACAAATG 
AGACCCCCCA AACCCAAGAG GAGGAAGATC TCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005601535.1
CDS	916..6114
Translation	MNGGKECDGGDKDGGLPAVQVPVGWQRRVDQNGVLYVSPSGSLLSCLEQVKTYLLTDGTCKCGLECPLILPKVFNFDPGAAVKQRTAEDVKADEDVTKLCIHKRKIIAVATLHKSMEAPHPSLVLTSPGGGTNATPVVPSRAATPRSVRNKSHEGITNSVMPECKNPFKLMIGSSNAMGRLYVQELPGSQQQELHPVYPRQRLGSSEHGQKSPFRGSHGGMPSPASSGSQIYGDGSISPRTDPLGSPDVFTRNNPGFHGAPNSSPIHLSRTPLSPPSVMLHGSPVQSSCAMAGRTNIPLSPTLTTKSPVMKKPMCNFSSNMEIPRAMFHHKPPQGPPPPPPPSCALQKKPLTSEKDPLGILDPIPSKPVNQNPVIINPTSFHSNVHSQVPVMNVSMPPAVVPLPSNLPLPTVKPGHMNHGSHVQRVQHSASTSLSPSPVTSPVHMMGTGIGRIEASPQRSRSSSTSSDHGNFMMPPLGPQATCSGIKVPPRSPRSTIGSPRPSMPSSPSTKSDGHHQYKDIPNPLIAGMSNVLNTPSSAAFPTASAGSGSVKSQPGLLGMPLNQILNQHNAASFPASSLLSAAAKAQLANQNKLAGNNNSSSSNSGAVAGSGNTEGHSTLNTMFPPTANMLLPTGEGQSGRAALRDKLMSQQKDSLRKRKQPPTTVLSLLRQSQMDSSAVPKPGPDLLRKQGQGSFPISSMSQLLQSMSCQSSHLSSNSAPGCGGSNTALPCSANQLHFTDPNMNSSVLQNSLTQNIPLRGEAVHCHNANTNFVHSNSPVPNHHLAGLINQIQASGNCGMLSQSGMALGNSLHPNPPQSRISTSSTPVIPNSIVSSYNQTSSEAGISGPSSSIAIAGTNHPAITKTTSVLQDGVIVTTAAGNPLQSQLPIGSDFPFVGQEHALHFPSNSTSNNHLPHPLNPSLLSSLPISLPVNQQHLLNQNLLNILQPSAGEGKSEINLHPLGFLNPNVNAALAFLSGDMDGQVLQPVHFQLLAALLQNQAQAAAMLPLPSFNLTISDLLQQQNTPLPSLTQMTAPPDHLPSNQSENSRAETLLTSPLGNPLPSFAGSDTTFNPLFLPAVTGASGLMALNPQLLGGVLNSASANTANHPEVSIATSSQATTTTTTTSSAVAALTVSTLGGTAVVSMAETLLNISNNAGNTPGPAKLNSNSVVPQLLNPLLGTGLLGDMSSINNTLNNHQLTHLQSLLNNNQMFPPNQQQQQLLQGYQNLQAFQGQSAIPCPANNNPMACLFQNFQVRMQEDATLLNKRISTQPGLTALPENPNTTLPPFQDTSCELQPRIDPSLGQQVKDGLVMGGQGDASVDAIYKAVVDAASKGMQVVITTAVNSTTQISPIPALSAMSAFTASIGDPLSLPSAVSAVIHGRNMGSVDHDGRLRNARGARLPRNLDHGKNSNEGDGFEYFKAASCHTSKKQWDGEQSPGGERNRWKCEEFLDHPGHIHSSPCHERSNNVSTLPFLPGEQHPILLPPRNCQGDKILEENFRYNNYKRTMMTFKERLENTVERCAHINGNRPRQSRGFGELLSTTRQDLVLEEQSPSSSNSLESSLVKDYIHYNGDFNAKSINGCVPSPSDAKSISSEDDLRNPDSPSSNELIHYRPRTFNVGDLVWGQIKGLTSWPGKLVREDDVHNSCQQSPEEGKVEPEKLKTLTEGLEAYSRARKRNRKSGKLNNHLEAAIHEAMSELDKMSGTVHQIPQGDRQMRPPKPKRRKISR

Target ORF information:

RefSeq Version	XM_005601478.2
Organism	Equus caballus(horse)
Definition	Equus caballus methyl-CpG binding domain protein 5 (MBD5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005601478.2

ORF Insert Sequence:

ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTGTACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGACGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATCAT TGCAGTGGCA ACACTTCATA AAAGCATGGA AGCTCCACAT 
CCTTCACTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA 
CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
ATGCCCAGCC CAGCGTCGTC AGGTTCCCAG ATATATGGAG ATGGCTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAGTAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACCAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAAGTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACTTCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACTAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA 
TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG 
GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAATAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA CAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGCGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA 
CAGCCACCTA CCACAGTGTT GAGTTTGCTC AGACAGTCGC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCCGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTGCC 
CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAATA TGAACTCCAG TGTTCTTCAG AATTCACTGA CACAGAACAT ACCTTTAAGA 
GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC 
CCGAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG 
CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA 
ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA 
AGTTCTGAAG CAGGCATTTC AGGACCATCA TCCTCCATAG CCATAGCTGG CACCAACCAC 
CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TTATAGTCAC CACCGCAGCT 
GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGATT TTCCCTTTGT TGGCCAGGAG 
CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC 
CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC 
CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC 
CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC 
ATGGATGGGC AGGTATTGCA ACCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC 
CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT 
TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT 
TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG 
AACCCTTTAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT 
GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC 
TCGGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA 
ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT 
GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT 
ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGGTGC CACAGCTACT TAACCCTCTA 
CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA 
CTGACTCATC TACAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAACAG 
CAGCAACTTC TTCAGGGGTA CCAGAACCTC CAGGCATTTC AAGGACAGTC CGCAATTCCT 
TGCCCAGCTA ACAATAACCC CATGGCTTGT CTGTTTCAGA ACTTTCAGGT GAGGATGCAG 
GAAGATGCAA CTCTCCTAAA CAAAAGAATA AGCACTCAGC CGGGGCTCAC AGCACTTCCT 
GAGAATCCGA ACACTACACT TCCGCCTTTC CAAGATACAT CTTGTGAGTT GCAACCGAGG 
ATTGACCCAT CTCTTGGTCA GCAGGTGAAG GATGGCCTTG TTATGGGTGG CCAAGGTGAT 
GCTTCTGTAG ATGCCATTTA CAAGGCAGTT GTTGATGCAG CCAGCAAAGG AATGCAGGTT 
GTCATCACCA CTGCAGTCAA CAGTACAACT CAGATCAGCC CCATTCCAGC TCTGAGTGCC 
ATGAGTGCCT TCACTGCTTC GATTGGTGAC CCGTTAAGTC TCCCCAGTGC TGTCAGTGCG 
GTCATTCATG GACGAAACAT GGGCAGTGTC GATCATGATG GTAGGCTGAG GAATGCAAGA 
GGGGCCCGGC TGCCCAGGAA TCTGGACCAT GGGAAAAACT CAAATGAAGG AGATGGGTTT 
GAATATTTCA AGGCAGCAAG TTGCCACACA TCCAAAAAAC AGTGGGATGG GGAGCAAAGC 
CCTGGAGGGG AACGAAACAG GTGGAAATGC GAGGAATTTT TAGATCATCC AGGCCATATC 
CACAGTAGTC CTTGTCATGA AAGGTCCAAT AATGTCTCTA CACTGCCATT TCTGCCTGGG 
GAACAGCACC CAATACTGTT ACCACCAAGA AACTGTCAAG GGGATAAAAT TCTGGAGGAA 
AATTTCAGGT ATAATAACTA CAAAAGAACT ATGATGACTT TTAAGGAGAG ACTAGAGAAC 
ACTGTGGAAA GATGTGCACA CATTAATGGG AACAGACCTC GACAGAGTCG GGGCTTTGGA 
GAGCTGCTGA GTACCACAAG GCAAGACCTC GTCCTAGAGG AGCAGTCTCC AAGCTCCTCA 
AATAGTTTGG AAAGTTCTCT GGTCAAAGAC TACATCCATT ATAATGGAGA CTTTAATGCC 
AAAAGCATTA ATGGGTGTGT GCCTAGCCCT TCAGACGCTA AAAGCATTAG TAGTGAAGAT 
GACCTAAGGA ATCCAGACTC CCCCTCTTCA AATGAGTTGA TACATTATAG GCCAAGGACG 
TTCAACGTTG GCGACTTGGT CTGGGGCCAA ATCAAAGGAC TTACTTCCTG GCCTGGAAAA 
TTAGTAAGAG AAGACGACGT TCACAATTCA TGTCAACAAA GCCCCGAGGA AGGGAAGGTG 
GAGCCGGAGA AGTTGAAGAC ACTAACAGAA GGTTTAGAAG CCTACAGTCG AGCCCGGAAA 
AGGAACAGAA AAAGTGGAAA GCTAAATAAC CATTTAGAAG CTGCTATTCA TGAGGCCATG 
AGTGAACTGG ACAAAATGTC TGGGACTGTT CACCAAATCC CACAGGGAGA CAGACAAATG 
AGACCCCCCA AACCCAAGAG GAGGAAGATC TCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD5 ( XM_005601478.3 ) cDNA ORF clone, Equus caballus(horse) -> XP_005601535.1 Equus caballus methyl-CpG binding domain protein 5 (MBD5), transcript variant X1, mRNA.

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CloneID	OHb08212
Clone ID Related Accession (Same CDS sequence)	XM_014732456.1 , XM_023622554.1 , XM_005601478.2 , XM_005601478.3 , XM_014732456.2
Accession Version	XM_005601478.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	methyl-CpG-binding domain protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_005601478.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTGTACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGACGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATCAT TGCAGTGGCA ACACTTCATA AAAGCATGGA AGCTCCACAT 
CCTTCACTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA 
CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
ATGCCCAGCC CAGCGTCGTC AGGTTCCCAG ATATATGGAG ATGGCTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAGTAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACCAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAAGTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACTTCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACTAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA 
TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG 
GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAATAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA CAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGCGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA 
CAGCCACCTA CCACAGTGTT GAGTTTGCTC AGACAGTCGC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCCGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTGCC 
CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAATA TGAACTCCAG TGTTCTTCAG AATTCACTGA CACAGAACAT ACCTTTAAGA 
GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC 
CCGAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG 
CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA 
ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA 
AGTTCTGAAG CAGGCATTTC AGGACCATCA TCCTCCATAG CCATAGCTGG CACCAACCAC 
CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TTATAGTCAC CACCGCAGCT 
GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGATT TTCCCTTTGT TGGCCAGGAG 
CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC 
CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC 
CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC 
CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC 
ATGGATGGGC AGGTATTGCA ACCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC 
CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT 
TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT 
TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG 
AACCCTTTAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT 
GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC 
TCGGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA 
ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT 
GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT 
ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGGTGC CACAGCTACT TAACCCTCTA 
CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA 
CTGACTCATC TACAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAACAG 
CAGCAACTTC TTCAGGGGTA CCAGAACCTC CAGGCATTTC AAGGACAGTC CGCAATTCCT 
TGCCCAGCTA ACAATAACCC CATGGCTTGT CTGTTTCAGA ACTTTCAGGT GAGGATGCAG 
GAAGATGCAA CTCTCCTAAA CAAAAGAATA AGCACTCAGC CGGGGCTCAC AGCACTTCCT 
GAGAATCCGA ACACTACACT TCCGCCTTTC CAAGATACAT CTTGTGAGTT GCAACCGAGG 
ATTGACCCAT CTCTTGGTCA GCAGGTGAAG GATGGCCTTG TTATGGGTGG CCAAGGTGAT 
GCTTCTGTAG ATGCCATTTA CAAGGCAGTT GTTGATGCAG CCAGCAAAGG AATGCAGGTT 
GTCATCACCA CTGCAGTCAA CAGTACAACT CAGATCAGCC CCATTCCAGC TCTGAGTGCC 
ATGAGTGCCT TCACTGCTTC GATTGGTGAC CCGTTAAGTC TCCCCAGTGC TGTCAGTGCG 
GTCATTCATG GACGAAACAT GGGCAGTGTC GATCATGATG GTAGGCTGAG GAATGCAAGA 
GGGGCCCGGC TGCCCAGGAA TCTGGACCAT GGGAAAAACT CAAATGAAGG AGATGGGTTT 
GAATATTTCA AGGCAGCAAG TTGCCACACA TCCAAAAAAC AGTGGGATGG GGAGCAAAGC 
CCTGGAGGGG AACGAAACAG GTGGAAATGC GAGGAATTTT TAGATCATCC AGGCCATATC 
CACAGTAGTC CTTGTCATGA AAGGTCCAAT AATGTCTCTA CACTGCCATT TCTGCCTGGG 
GAACAGCACC CAATACTGTT ACCACCAAGA AACTGTCAAG GGGATAAAAT TCTGGAGGAA 
AATTTCAGGT ATAATAACTA CAAAAGAACT ATGATGACTT TTAAGGAGAG ACTAGAGAAC 
ACTGTGGAAA GATGTGCACA CATTAATGGG AACAGACCTC GACAGAGTCG GGGCTTTGGA 
GAGCTGCTGA GTACCACAAG GCAAGACCTC GTCCTAGAGG AGCAGTCTCC AAGCTCCTCA 
AATAGTTTGG AAAGTTCTCT GGTCAAAGAC TACATCCATT ATAATGGAGA CTTTAATGCC 
AAAAGCATTA ATGGGTGTGT GCCTAGCCCT TCAGACGCTA AAAGCATTAG TAGTGAAGAT 
GACCTAAGGA ATCCAGACTC CCCCTCTTCA AATGAGTTGA TACATTATAG GCCAAGGACG 
TTCAACGTTG GCGACTTGGT CTGGGGCCAA ATCAAAGGAC TTACTTCCTG GCCTGGAAAA 
TTAGTAAGAG AAGACGACGT TCACAATTCA TGTCAACAAA GCCCCGAGGA AGGGAAGGTG 
GAGCCGGAGA AGTTGAAGAC ACTAACAGAA GGTTTAGAAG CCTACAGTCG AGCCCGGAAA 
AGGAACAGAA AAAGTGGAAA GCTAAATAAC CATTTAGAAG CTGCTATTCA TGAGGCCATG 
AGTGAACTGG ACAAAATGTC TGGGACTGTT CACCAAATCC CACAGGGAGA CAGACAAATG 
AGACCCCCCA AACCCAAGAG GAGGAAGATC TCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005601535.1
CDS	1724..6922
Translation	MNGGKECDGGDKDGGLPAVQVPVGWQRRVDQNGVLYVSPSGSLLSCLEQVKTYLLTDGTCKCGLECPLILPKVFNFDPGAAVKQRTAEDVKADEDVTKLCIHKRKIIAVATLHKSMEAPHPSLVLTSPGGGTNATPVVPSRAATPRSVRNKSHEGITNSVMPECKNPFKLMIGSSNAMGRLYVQELPGSQQQELHPVYPRQRLGSSEHGQKSPFRGSHGGMPSPASSGSQIYGDGSISPRTDPLGSPDVFTRNNPGFHGAPNSSPIHLSRTPLSPPSVMLHGSPVQSSCAMAGRTNIPLSPTLTTKSPVMKKPMCNFSSNMEIPRAMFHHKPPQGPPPPPPPSCALQKKPLTSEKDPLGILDPIPSKPVNQNPVIINPTSFHSNVHSQVPVMNVSMPPAVVPLPSNLPLPTVKPGHMNHGSHVQRVQHSASTSLSPSPVTSPVHMMGTGIGRIEASPQRSRSSSTSSDHGNFMMPPLGPQATCSGIKVPPRSPRSTIGSPRPSMPSSPSTKSDGHHQYKDIPNPLIAGMSNVLNTPSSAAFPTASAGSGSVKSQPGLLGMPLNQILNQHNAASFPASSLLSAAAKAQLANQNKLAGNNNSSSSNSGAVAGSGNTEGHSTLNTMFPPTANMLLPTGEGQSGRAALRDKLMSQQKDSLRKRKQPPTTVLSLLRQSQMDSSAVPKPGPDLLRKQGQGSFPISSMSQLLQSMSCQSSHLSSNSAPGCGGSNTALPCSANQLHFTDPNMNSSVLQNSLTQNIPLRGEAVHCHNANTNFVHSNSPVPNHHLAGLINQIQASGNCGMLSQSGMALGNSLHPNPPQSRISTSSTPVIPNSIVSSYNQTSSEAGISGPSSSIAIAGTNHPAITKTTSVLQDGVIVTTAAGNPLQSQLPIGSDFPFVGQEHALHFPSNSTSNNHLPHPLNPSLLSSLPISLPVNQQHLLNQNLLNILQPSAGEGKSEINLHPLGFLNPNVNAALAFLSGDMDGQVLQPVHFQLLAALLQNQAQAAAMLPLPSFNLTISDLLQQQNTPLPSLTQMTAPPDHLPSNQSENSRAETLLTSPLGNPLPSFAGSDTTFNPLFLPAVTGASGLMALNPQLLGGVLNSASANTANHPEVSIATSSQATTTTTTTSSAVAALTVSTLGGTAVVSMAETLLNISNNAGNTPGPAKLNSNSVVPQLLNPLLGTGLLGDMSSINNTLNNHQLTHLQSLLNNNQMFPPNQQQQQLLQGYQNLQAFQGQSAIPCPANNNPMACLFQNFQVRMQEDATLLNKRISTQPGLTALPENPNTTLPPFQDTSCELQPRIDPSLGQQVKDGLVMGGQGDASVDAIYKAVVDAASKGMQVVITTAVNSTTQISPIPALSAMSAFTASIGDPLSLPSAVSAVIHGRNMGSVDHDGRLRNARGARLPRNLDHGKNSNEGDGFEYFKAASCHTSKKQWDGEQSPGGERNRWKCEEFLDHPGHIHSSPCHERSNNVSTLPFLPGEQHPILLPPRNCQGDKILEENFRYNNYKRTMMTFKERLENTVERCAHINGNRPRQSRGFGELLSTTRQDLVLEEQSPSSSNSLESSLVKDYIHYNGDFNAKSINGCVPSPSDAKSISSEDDLRNPDSPSSNELIHYRPRTFNVGDLVWGQIKGLTSWPGKLVREDDVHNSCQQSPEEGKVEPEKLKTLTEGLEAYSRARKRNRKSGKLNNHLEAAIHEAMSELDKMSGTVHQIPQGDRQMRPPKPKRRKISR

Target ORF information:

RefSeq Version	XM_005601478.3
Organism	Equus caballus(horse)
Definition	Equus caballus methyl-CpG binding domain protein 5 (MBD5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005601478.3

ORF Insert Sequence:

ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTGTACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGACGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATCAT TGCAGTGGCA ACACTTCATA AAAGCATGGA AGCTCCACAT 
CCTTCACTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA 
CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
ATGCCCAGCC CAGCGTCGTC AGGTTCCCAG ATATATGGAG ATGGCTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAGTAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACCAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAAGTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACTTCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACTAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA 
TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG 
GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAATAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA CAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGCGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA 
CAGCCACCTA CCACAGTGTT GAGTTTGCTC AGACAGTCGC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCCGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTGCC 
CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAATA TGAACTCCAG TGTTCTTCAG AATTCACTGA CACAGAACAT ACCTTTAAGA 
GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC 
CCGAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG 
CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA 
ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA 
AGTTCTGAAG CAGGCATTTC AGGACCATCA TCCTCCATAG CCATAGCTGG CACCAACCAC 
CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TTATAGTCAC CACCGCAGCT 
GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGATT TTCCCTTTGT TGGCCAGGAG 
CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC 
CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC 
CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC 
CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC 
ATGGATGGGC AGGTATTGCA ACCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC 
CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT 
TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT 
TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG 
AACCCTTTAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT 
GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC 
TCGGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA 
ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT 
GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT 
ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGGTGC CACAGCTACT TAACCCTCTA 
CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA 
CTGACTCATC TACAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAACAG 
CAGCAACTTC TTCAGGGGTA CCAGAACCTC CAGGCATTTC AAGGACAGTC CGCAATTCCT 
TGCCCAGCTA ACAATAACCC CATGGCTTGT CTGTTTCAGA ACTTTCAGGT GAGGATGCAG 
GAAGATGCAA CTCTCCTAAA CAAAAGAATA AGCACTCAGC CGGGGCTCAC AGCACTTCCT 
GAGAATCCGA ACACTACACT TCCGCCTTTC CAAGATACAT CTTGTGAGTT GCAACCGAGG 
ATTGACCCAT CTCTTGGTCA GCAGGTGAAG GATGGCCTTG TTATGGGTGG CCAAGGTGAT 
GCTTCTGTAG ATGCCATTTA CAAGGCAGTT GTTGATGCAG CCAGCAAAGG AATGCAGGTT 
GTCATCACCA CTGCAGTCAA CAGTACAACT CAGATCAGCC CCATTCCAGC TCTGAGTGCC 
ATGAGTGCCT TCACTGCTTC GATTGGTGAC CCGTTAAGTC TCCCCAGTGC TGTCAGTGCG 
GTCATTCATG GACGAAACAT GGGCAGTGTC GATCATGATG GTAGGCTGAG GAATGCAAGA 
GGGGCCCGGC TGCCCAGGAA TCTGGACCAT GGGAAAAACT CAAATGAAGG AGATGGGTTT 
GAATATTTCA AGGCAGCAAG TTGCCACACA TCCAAAAAAC AGTGGGATGG GGAGCAAAGC 
CCTGGAGGGG AACGAAACAG GTGGAAATGC GAGGAATTTT TAGATCATCC AGGCCATATC 
CACAGTAGTC CTTGTCATGA AAGGTCCAAT AATGTCTCTA CACTGCCATT TCTGCCTGGG 
GAACAGCACC CAATACTGTT ACCACCAAGA AACTGTCAAG GGGATAAAAT TCTGGAGGAA 
AATTTCAGGT ATAATAACTA CAAAAGAACT ATGATGACTT TTAAGGAGAG ACTAGAGAAC 
ACTGTGGAAA GATGTGCACA CATTAATGGG AACAGACCTC GACAGAGTCG GGGCTTTGGA 
GAGCTGCTGA GTACCACAAG GCAAGACCTC GTCCTAGAGG AGCAGTCTCC AAGCTCCTCA 
AATAGTTTGG AAAGTTCTCT GGTCAAAGAC TACATCCATT ATAATGGAGA CTTTAATGCC 
AAAAGCATTA ATGGGTGTGT GCCTAGCCCT TCAGACGCTA AAAGCATTAG TAGTGAAGAT 
GACCTAAGGA ATCCAGACTC CCCCTCTTCA AATGAGTTGA TACATTATAG GCCAAGGACG 
TTCAACGTTG GCGACTTGGT CTGGGGCCAA ATCAAAGGAC TTACTTCCTG GCCTGGAAAA 
TTAGTAAGAG AAGACGACGT TCACAATTCA TGTCAACAAA GCCCCGAGGA AGGGAAGGTG 
GAGCCGGAGA AGTTGAAGAC ACTAACAGAA GGTTTAGAAG CCTACAGTCG AGCCCGGAAA 
AGGAACAGAA AAAGTGGAAA GCTAAATAAC CATTTAGAAG CTGCTATTCA TGAGGCCATG 
AGTGAACTGG ACAAAATGTC TGGGACTGTT CACCAAATCC CACAGGGAGA CAGACAAATG 
AGACCCCCCA AACCCAAGAG GAGGAAGATC TCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD5 ( XM_014732456.2 ) cDNA ORF clone, Equus caballus(horse) -> XP_014587942.1 Equus caballus methyl-CpG binding domain protein 5 (MBD5), transcript variant X3, mRNA.

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CloneID	OHb08212
Clone ID Related Accession (Same CDS sequence)	XM_014732456.1 , XM_023622554.1 , XM_005601478.2 , XM_005601478.3 , XM_014732456.2
Accession Version	XM_014732456.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	methyl-CpG-binding domain protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_014732456.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTGTACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGACGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATCAT TGCAGTGGCA ACACTTCATA AAAGCATGGA AGCTCCACAT 
CCTTCACTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA 
CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
ATGCCCAGCC CAGCGTCGTC AGGTTCCCAG ATATATGGAG ATGGCTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAGTAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACCAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAAGTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACTTCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACTAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA 
TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG 
GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAATAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA CAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGCGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA 
CAGCCACCTA CCACAGTGTT GAGTTTGCTC AGACAGTCGC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCCGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTGCC 
CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAATA TGAACTCCAG TGTTCTTCAG AATTCACTGA CACAGAACAT ACCTTTAAGA 
GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC 
CCGAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG 
CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA 
ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA 
AGTTCTGAAG CAGGCATTTC AGGACCATCA TCCTCCATAG CCATAGCTGG CACCAACCAC 
CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TTATAGTCAC CACCGCAGCT 
GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGATT TTCCCTTTGT TGGCCAGGAG 
CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC 
CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC 
CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC 
CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC 
ATGGATGGGC AGGTATTGCA ACCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC 
CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT 
TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT 
TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG 
AACCCTTTAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT 
GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC 
TCGGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA 
ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT 
GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT 
ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGGTGC CACAGCTACT TAACCCTCTA 
CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA 
CTGACTCATC TACAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAACAG 
CAGCAACTTC TTCAGGGGTA CCAGAACCTC CAGGCATTTC AAGGACAGTC CGCAATTCCT 
TGCCCAGCTA ACAATAACCC CATGGCTTGT CTGTTTCAGA ACTTTCAGGT GAGGATGCAG 
GAAGATGCAA CTCTCCTAAA CAAAAGAATA AGCACTCAGC CGGGGCTCAC AGCACTTCCT 
GAGAATCCGA ACACTACACT TCCGCCTTTC CAAGATACAT CTTGTGAGTT GCAACCGAGG 
ATTGACCCAT CTCTTGGTCA GCAGGTGAAG GATGGCCTTG TTATGGGTGG CCAAGGTGAT 
GCTTCTGTAG ATGCCATTTA CAAGGCAGTT GTTGATGCAG CCAGCAAAGG AATGCAGGTT 
GTCATCACCA CTGCAGTCAA CAGTACAACT CAGATCAGCC CCATTCCAGC TCTGAGTGCC 
ATGAGTGCCT TCACTGCTTC GATTGGTGAC CCGTTAAGTC TCCCCAGTGC TGTCAGTGCG 
GTCATTCATG GACGAAACAT GGGCAGTGTC GATCATGATG GTAGGCTGAG GAATGCAAGA 
GGGGCCCGGC TGCCCAGGAA TCTGGACCAT GGGAAAAACT CAAATGAAGG AGATGGGTTT 
GAATATTTCA AGGCAGCAAG TTGCCACACA TCCAAAAAAC AGTGGGATGG GGAGCAAAGC 
CCTGGAGGGG AACGAAACAG GTGGAAATGC GAGGAATTTT TAGATCATCC AGGCCATATC 
CACAGTAGTC CTTGTCATGA AAGGTCCAAT AATGTCTCTA CACTGCCATT TCTGCCTGGG 
GAACAGCACC CAATACTGTT ACCACCAAGA AACTGTCAAG GGGATAAAAT TCTGGAGGAA 
AATTTCAGGT ATAATAACTA CAAAAGAACT ATGATGACTT TTAAGGAGAG ACTAGAGAAC 
ACTGTGGAAA GATGTGCACA CATTAATGGG AACAGACCTC GACAGAGTCG GGGCTTTGGA 
GAGCTGCTGA GTACCACAAG GCAAGACCTC GTCCTAGAGG AGCAGTCTCC AAGCTCCTCA 
AATAGTTTGG AAAGTTCTCT GGTCAAAGAC TACATCCATT ATAATGGAGA CTTTAATGCC 
AAAAGCATTA ATGGGTGTGT GCCTAGCCCT TCAGACGCTA AAAGCATTAG TAGTGAAGAT 
GACCTAAGGA ATCCAGACTC CCCCTCTTCA AATGAGTTGA TACATTATAG GCCAAGGACG 
TTCAACGTTG GCGACTTGGT CTGGGGCCAA ATCAAAGGAC TTACTTCCTG GCCTGGAAAA 
TTAGTAAGAG AAGACGACGT TCACAATTCA TGTCAACAAA GCCCCGAGGA AGGGAAGGTG 
GAGCCGGAGA AGTTGAAGAC ACTAACAGAA GGTTTAGAAG CCTACAGTCG AGCCCGGAAA 
AGGAACAGAA AAAGTGGAAA GCTAAATAAC CATTTAGAAG CTGCTATTCA TGAGGCCATG 
AGTGAACTGG ACAAAATGTC TGGGACTGTT CACCAAATCC CACAGGGAGA CAGACAAATG 
AGACCCCCCA AACCCAAGAG GAGGAAGATC TCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014587942.1
CDS	1550..6748
Translation	MNGGKECDGGDKDGGLPAVQVPVGWQRRVDQNGVLYVSPSGSLLSCLEQVKTYLLTDGTCKCGLECPLILPKVFNFDPGAAVKQRTAEDVKADEDVTKLCIHKRKIIAVATLHKSMEAPHPSLVLTSPGGGTNATPVVPSRAATPRSVRNKSHEGITNSVMPECKNPFKLMIGSSNAMGRLYVQELPGSQQQELHPVYPRQRLGSSEHGQKSPFRGSHGGMPSPASSGSQIYGDGSISPRTDPLGSPDVFTRNNPGFHGAPNSSPIHLSRTPLSPPSVMLHGSPVQSSCAMAGRTNIPLSPTLTTKSPVMKKPMCNFSSNMEIPRAMFHHKPPQGPPPPPPPSCALQKKPLTSEKDPLGILDPIPSKPVNQNPVIINPTSFHSNVHSQVPVMNVSMPPAVVPLPSNLPLPTVKPGHMNHGSHVQRVQHSASTSLSPSPVTSPVHMMGTGIGRIEASPQRSRSSSTSSDHGNFMMPPLGPQATCSGIKVPPRSPRSTIGSPRPSMPSSPSTKSDGHHQYKDIPNPLIAGMSNVLNTPSSAAFPTASAGSGSVKSQPGLLGMPLNQILNQHNAASFPASSLLSAAAKAQLANQNKLAGNNNSSSSNSGAVAGSGNTEGHSTLNTMFPPTANMLLPTGEGQSGRAALRDKLMSQQKDSLRKRKQPPTTVLSLLRQSQMDSSAVPKPGPDLLRKQGQGSFPISSMSQLLQSMSCQSSHLSSNSAPGCGGSNTALPCSANQLHFTDPNMNSSVLQNSLTQNIPLRGEAVHCHNANTNFVHSNSPVPNHHLAGLINQIQASGNCGMLSQSGMALGNSLHPNPPQSRISTSSTPVIPNSIVSSYNQTSSEAGISGPSSSIAIAGTNHPAITKTTSVLQDGVIVTTAAGNPLQSQLPIGSDFPFVGQEHALHFPSNSTSNNHLPHPLNPSLLSSLPISLPVNQQHLLNQNLLNILQPSAGEGKSEINLHPLGFLNPNVNAALAFLSGDMDGQVLQPVHFQLLAALLQNQAQAAAMLPLPSFNLTISDLLQQQNTPLPSLTQMTAPPDHLPSNQSENSRAETLLTSPLGNPLPSFAGSDTTFNPLFLPAVTGASGLMALNPQLLGGVLNSASANTANHPEVSIATSSQATTTTTTTSSAVAALTVSTLGGTAVVSMAETLLNISNNAGNTPGPAKLNSNSVVPQLLNPLLGTGLLGDMSSINNTLNNHQLTHLQSLLNNNQMFPPNQQQQQLLQGYQNLQAFQGQSAIPCPANNNPMACLFQNFQVRMQEDATLLNKRISTQPGLTALPENPNTTLPPFQDTSCELQPRIDPSLGQQVKDGLVMGGQGDASVDAIYKAVVDAASKGMQVVITTAVNSTTQISPIPALSAMSAFTASIGDPLSLPSAVSAVIHGRNMGSVDHDGRLRNARGARLPRNLDHGKNSNEGDGFEYFKAASCHTSKKQWDGEQSPGGERNRWKCEEFLDHPGHIHSSPCHERSNNVSTLPFLPGEQHPILLPPRNCQGDKILEENFRYNNYKRTMMTFKERLENTVERCAHINGNRPRQSRGFGELLSTTRQDLVLEEQSPSSSNSLESSLVKDYIHYNGDFNAKSINGCVPSPSDAKSISSEDDLRNPDSPSSNELIHYRPRTFNVGDLVWGQIKGLTSWPGKLVREDDVHNSCQQSPEEGKVEPEKLKTLTEGLEAYSRARKRNRKSGKLNNHLEAAIHEAMSELDKMSGTVHQIPQGDRQMRPPKPKRRKISR

Target ORF information:

RefSeq Version	XM_014732456.2
Organism	Equus caballus(horse)
Definition	Equus caballus methyl-CpG binding domain protein 5 (MBD5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014732456.2

ORF Insert Sequence:

ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTGTACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGACGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATCAT TGCAGTGGCA ACACTTCATA AAAGCATGGA AGCTCCACAT 
CCTTCACTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA 
CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
ATGCCCAGCC CAGCGTCGTC AGGTTCCCAG ATATATGGAG ATGGCTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAGTAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACCAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAAGTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACTTCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACTAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA 
TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG 
GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAATAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA CAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGCGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA 
CAGCCACCTA CCACAGTGTT GAGTTTGCTC AGACAGTCGC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCCGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTGCC 
CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAATA TGAACTCCAG TGTTCTTCAG AATTCACTGA CACAGAACAT ACCTTTAAGA 
GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC 
CCGAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG 
CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA 
ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA 
AGTTCTGAAG CAGGCATTTC AGGACCATCA TCCTCCATAG CCATAGCTGG CACCAACCAC 
CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TTATAGTCAC CACCGCAGCT 
GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGATT TTCCCTTTGT TGGCCAGGAG 
CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC 
CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC 
CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC 
CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC 
ATGGATGGGC AGGTATTGCA ACCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC 
CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT 
TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT 
TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG 
AACCCTTTAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT 
GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC 
TCGGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA 
ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT 
GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT 
ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGGTGC CACAGCTACT TAACCCTCTA 
CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA 
CTGACTCATC TACAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAACAG 
CAGCAACTTC TTCAGGGGTA CCAGAACCTC CAGGCATTTC AAGGACAGTC CGCAATTCCT 
TGCCCAGCTA ACAATAACCC CATGGCTTGT CTGTTTCAGA ACTTTCAGGT GAGGATGCAG 
GAAGATGCAA CTCTCCTAAA CAAAAGAATA AGCACTCAGC CGGGGCTCAC AGCACTTCCT 
GAGAATCCGA ACACTACACT TCCGCCTTTC CAAGATACAT CTTGTGAGTT GCAACCGAGG 
ATTGACCCAT CTCTTGGTCA GCAGGTGAAG GATGGCCTTG TTATGGGTGG CCAAGGTGAT 
GCTTCTGTAG ATGCCATTTA CAAGGCAGTT GTTGATGCAG CCAGCAAAGG AATGCAGGTT 
GTCATCACCA CTGCAGTCAA CAGTACAACT CAGATCAGCC CCATTCCAGC TCTGAGTGCC 
ATGAGTGCCT TCACTGCTTC GATTGGTGAC CCGTTAAGTC TCCCCAGTGC TGTCAGTGCG 
GTCATTCATG GACGAAACAT GGGCAGTGTC GATCATGATG GTAGGCTGAG GAATGCAAGA 
GGGGCCCGGC TGCCCAGGAA TCTGGACCAT GGGAAAAACT CAAATGAAGG AGATGGGTTT 
GAATATTTCA AGGCAGCAAG TTGCCACACA TCCAAAAAAC AGTGGGATGG GGAGCAAAGC 
CCTGGAGGGG AACGAAACAG GTGGAAATGC GAGGAATTTT TAGATCATCC AGGCCATATC 
CACAGTAGTC CTTGTCATGA AAGGTCCAAT AATGTCTCTA CACTGCCATT TCTGCCTGGG 
GAACAGCACC CAATACTGTT ACCACCAAGA AACTGTCAAG GGGATAAAAT TCTGGAGGAA 
AATTTCAGGT ATAATAACTA CAAAAGAACT ATGATGACTT TTAAGGAGAG ACTAGAGAAC 
ACTGTGGAAA GATGTGCACA CATTAATGGG AACAGACCTC GACAGAGTCG GGGCTTTGGA 
GAGCTGCTGA GTACCACAAG GCAAGACCTC GTCCTAGAGG AGCAGTCTCC AAGCTCCTCA 
AATAGTTTGG AAAGTTCTCT GGTCAAAGAC TACATCCATT ATAATGGAGA CTTTAATGCC 
AAAAGCATTA ATGGGTGTGT GCCTAGCCCT TCAGACGCTA AAAGCATTAG TAGTGAAGAT 
GACCTAAGGA ATCCAGACTC CCCCTCTTCA AATGAGTTGA TACATTATAG GCCAAGGACG 
TTCAACGTTG GCGACTTGGT CTGGGGCCAA ATCAAAGGAC TTACTTCCTG GCCTGGAAAA 
TTAGTAAGAG AAGACGACGT TCACAATTCA TGTCAACAAA GCCCCGAGGA AGGGAAGGTG 
GAGCCGGAGA AGTTGAAGAC ACTAACAGAA GGTTTAGAAG CCTACAGTCG AGCCCGGAAA 
AGGAACAGAA AAAGTGGAAA GCTAAATAAC CATTTAGAAG CTGCTATTCA TGAGGCCATG 
AGTGAACTGG ACAAAATGTC TGGGACTGTT CACCAAATCC CACAGGGAGA CAGACAAATG 
AGACCCCCCA AACCCAAGAG GAGGAAGATC TCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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MBD5 cDNA ORF clone, Equus caballus(horse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

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